The Integrin α1 A-domain Is a Ligand Binding Site for Collagens and Laminin*
- From the Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, University of Manchester, Manchester, M13 9PT, United Kingdom, the §Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, and the ¶Max-Planck-Institut für Biochemie, 82152 Martinsreid, Germany
Abstract
The integrin α1β1 is a cell surface receptor for collagens and laminin. The α1 subunit contains an A-domain, and the A-domains of other integrins are known to mediate ligand binding. To determine the role of the α1 A-domain in ligand binding and the extent to which it reproduced the ligand binding activity and specificity of the parent molecule, we produced recombinant α1 A-domain and tested its ability to bind collagens and laminin. In solid phase assays, the A-domain from α1 was found to bind to collagen I, collagen IV, and laminin in a largely cation-dependent manner. The α2 A-domain, from the α2β1 integrin, also bound to these ligands, but the binding hierarchy differed from that seen for α1. This is the first demonstration of laminin binding by A-domains. Specificity of A-domain-ligand binding was further investigated using the triple-helical proteolytic fragment of collagen IV, CB3, and its subfragments, F1 and F4. α1 A-domain bound to all three fragments, while the α2 A-domain bound CB3 less well and exhibited little binding to F1 and no binding to F4. These differences mirror previous reports of distinct integrin binding sites in collagen IV and for the first time identify a limited proteolytic fragment of a ligand that contains integrin A-domain binding activity. To gain insight into the contribution that the A-domain makes to ligand binding within the whole integrin heterodimer, we measured binding constants for A-domain-collagen interactions using surface plasmon resonance biosensor technology. The values obtained were similar to those reported for intact integrin binding, suggesting that the A-domain is the major collagen binding site in the α1β1 and α2β1 integrins.
Footnotes
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↵* This work was supported by grants from the Wellcome Trust.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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↵‡ Present address: Dept. Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037.
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↵‖ To whom correspondence should be addressed: Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, University of Manchester, 2.205 Stopford Bldg., Oxford Rd., Manchester, M13 9PT, UK. Tel.: 44-161-275-5071; Fax: 44-161-275-5082.
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↵1 The abbreviations used are: mAb, monoclonal antibody; RT-PCR, reverse transcriptase-polymerase chain reaction; GST, glutathione S-transferase; TBS, tris-buffered saline; ELISA, enzyme-linked immunosorbent assay; BSA, bovine serum albumin; PAGE, polyacrylamide gel electrophoresis; SPR, surface plasmon resonance; ABTS, 2′2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid).
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↵2 D. A. Calderwood, unpublished observation.
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↵3 J. Eble, unpublished data.
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- Received January 6, 1997.
- Revision received February 24, 1997.
