Oxidation inhibits substrate proteolysis by calpain I but not autolysis.

In this study, the effects of oxidation on calpain I autolysis and calpain-mediated proteolysis were examined. Calpain I was incubated with increasing concentrations of free calcium in the presence or absence of oxidant, and autolytic conversion of both the 80- and 30-kDa subunits was measured by immunoblotting utilizing monoclonal antibodies which recognize both autolyzed and non-autolyzed forms of each subunit, respectively. Autolytic conversion of the 80-kDa subunit of calpain I was not detected until free calcium concentration was greater than 40 μM, whereas autolysis of the 30-kDa subunit did not occur until the free calcium concentration was greater than 100 μM. In addition, autolytic conversion of either the 80- or 30-kDa subunit was not inhibited by the presence of oxidant. Calpain I activity was measured using the fluorescent peptide N-succinyl-L-leucyl-L-leucyl-L-valyl-L-tyrosine-7-amido-4-methylcoumarin or the microtubule-associated protein tau as substrate. Calpain I was found to have proteolytic activity at free calcium concentrations below that required for autolysis. Calpain I activity was strongly inhibited by oxidant at all calcium concentrations studied, suggesting that proteolytic activity of both the non-autolyzed 80-kDa and autolyzed 76-kDa forms was susceptible to oxidation. Interestingly, whereas oxidation did not inhibit autolytic conversion, the presence of high substrate concentrations did result in a significant reduction of autolysis without altering calpain proteolytic activity. Calpain I activity that had been inhibited by the presence of oxidant was recovered immediately by addition of the reducing agent dithiothreitol.

In this study, the effects of oxidation on calpain I autolysis and calpain-mediated proteolysis were examined. Calpain I was incubated with increasing concentrations of free calcium in the presence or absence of oxidant, and autolytic conversion of both the 80-and 30-kDa subunits was measured by immunoblotting utilizing monoclonal antibodies which recognize both autolyzed and non-autolyzed forms of each subunit, respectively. Autolytic conversion of the 80-kDa subunit of calpain I was not detected until free calcium concentration was greater than 40 M, whereas autolysis of the 30-kDa subunit did not occur until the free calcium concentration was greater than 100 M. In addition, autolytic conversion of either the 80-or 30-kDa subunit was not inhibited by the presence of oxidant. Calpain I activity was measured using the fluorescent peptide N-succinyl-L-leucyl-L-leucyl-L-valyl-L-tyrosine-7-amido-4-methylcoumarin or the microtubule-associated protein tau as substrate. Calpain I was found to have proteolytic activity at free calcium concentrations below that required for autolysis. Calpain I activity was strongly inhibited by oxidant at all calcium concentrations studied, suggesting that proteolytic activity of both the non-autolyzed 80-kDa and autolyzed 76-kDa forms was susceptible to oxidation. Interestingly, whereas oxidation did not inhibit autolytic conversion, the presence of high substrate concentrations did result in a significant reduction of autolysis without altering calpain proteolytic activity. Calpain I activity that had been inhibited by the presence of oxidant was recovered immediately by addition of the reducing agent dithiothreitol.
Calpains are a family of calcium-dependent thiol proteases that require both calcium and a reduced environment for activity. Calpains are present in virtually all vertebrate cells and have been postulated to play a role in many physiological processes (1)(2)(3)(4). Calpains I and II are ubiquitously expressed, whereas the remaining isoforms are tissue-specific and are found predominantly in muscle (5,6). Although homologous, calpains I and II require different concentrations of calcium for activity in vitro. Calpain II requires 200-1000 M calcium (7), and calpain I requires 3-50 M calcium (7) for half-maximal activity, a level which has been shown to be reached in the presynaptic terminals of neurons (8) and under pathological conditions (9). The focus of this study was calpain I because it is present in neurons and has been postulated to play a role in neuronal death associated with ischemia (10,11) and certain neurodegenerative disorders (12)(13)(14)(15).
Calpain I is a heterodimer composed of a unique 80-kDa catalytic subunit and a 30-kDa regulatory subunit which is identical to the 30-kDa subunit of calpain II (16). How these subunits interact and what regulatory mechanisms are involved in the process of calpain activation remain unresolved. Initially it was proposed that the 80-kDa form of calpain I was an inactive pro-enzyme that must undergo calcium-dependent conformational changes exposing the catalytic, thiol-protease domain, resulting in autolytic conversion to an intermediate 78-kDa form, then to a 76-kDa form in order to be an active protease (17). However, there is now substantial evidence to indicate that calpain I is active in its non-autolyzed 80-kDa form (7,18,19). The disparate findings concerning the proteolytic activity of the native 80-kDa form of calpain I may be due to the process of autolysis being modulated by more than calcium concentration alone (e.g. the presence of phospholipids, calpastatin, or other proteins) (20). It is also unclear as to the specific role of the 30-kDa noncatalytic subunit in modulating calpain autolysis and proteolytic activity. Several studies have shown that the calcium-dependent conversion of the 30-kDa to the 18-kDa form occurs subsequent to both autolysis of the 80-kDa subunit and proteolytic activity, suggesting conversion of the small subunit is unlikely to be required for calpain activation (21,22). However, it has also been demonstrated that the presence of the 30-kDa subunit is required to reconstitute a proteolytically active, bacterially expressed, 80-kDa calpain II subunit (23). Although this study suggests that the catalytic subunit is essential to "enhance" the activity of the catalytic subunit, other studies indicate that the role of the smaller subunit may be as a chaperone and aid in folding of the 80-kDa inactive conformation (19). It has also been hypothesized that the 30-kDa is an inhibitor of the 80-kDa subunit and that dissociation of the two subunits is the crucial activation event (19,24).
An interesting feature of calpain I-mediated proteolysis is that processing of most substrates is limited, resulting in the production of a few large polypeptide fragments. Generally, cleavage of enzyme substrates by calpain I results in a modification of activity rather than inactivation or complete digestion. For example, protein kinase C remains fully active after calpain cleavage but no longer requires calcium and phospholipid for activity (25). Likewise, calpain cleavage of calcineurin results in a phosphatase that is active but no longer requires calcium and calmodulin (26). Several structural proteins have also been identified as calpain substrates, including neurofilaments (27), spectrin (28,29), and microtubule-associated pro-teins (30,31), including tau (32), suggesting that calpain-mediated proteolysis may play a role in modulating cytoskeletal architecture.
The proteolysis of substrates by calpain I is likely to be affected by the redox state of the local environment. The mechanisms of calpain I-mediated hydrolysis of a peptide bond include the transfer of electrons between specific cysteine and histidine residues within the active site (33). For this transfer to occur these particular residues must be maintained in a properly charged state, which is affected by their microenvironment. Of the two residues, it is the cysteine residue that is more susceptible to oxidative inactivation (34) and therefore is likely to play a significant role in decreasing calpain I activity by an oxidizing environment. The effects of an oxidizing environment on calpain I activity are important to understand because oxidative stress has been linked to several pathological states in which calpain I has been suggested to play a role, including Alzheimer's disease (35,36) and ischemia (37,38).
In the present study, both calpain I autolysis and proteolytic activity were examined in the presence of oxidant at various calcium concentrations. These results suggest that calpain I is active in its native 80-kDa form, although the activity levels are lower than that observed for the 76-kDa form. Furthermore, calpain I-mediated proteolysis by either the 80-or 76-kDa form was inhibited by the presence of oxidant. However, oxidant had no effect on the autolytic conversion of either the 80-or 30-kDa calpain I subunits. Additionally, the rate of autolytic conversion of the 80-kDa to the 76-kDa subunit was reduced in the presence of high substrate concentrations. Finally, the oxidation-induced inhibition of calpain I proteolytic activity was rapidly reversed by the addition of the reducing agent dithiothreitol (DTT). 1
Free Calcium Determination-Free calcium concentrations greater than 100 M were determined using a calcium-sensitive ion selective probe (Orion Research model EA940) after calibration per manufacturer's instructions. Free calcium concentrations below 100 M were determined in all reaction mixtures using the calcium indicator dye, fura-2. Fura-2 was excited at alternating wavelengths of 340-and 380-nm using a 75-watt xenon light source, monochromators, and a chopper (cuvette-based PTI Deltascan System). Emitted wavelengths passed through a monochromator set at 510 nm before detection by a photometer. Data were stored and processed using PTI software. Calibration of fura-2 fluorescence was performed per PTI instructions where the ratio (R) of emitted signals at 340-and 380-nm excitation wavelengths provided an index of calcium concentration, which was estimated according to the equation (40) where K d is the effective dissociation constant of fura-2, R min and R max are the 340-to 380-nm ratios, and S f and S b are fluorescence values in the absence and presence of saturating calcium, respectively. The value of K d was calculated to be 210 nM (40).
Calpain Proteolysis and Autolysis Assays-All calpain assays were carried out at 25°C with 0.48 units/ml calpain I in a buffer containing 50 mM Hepes, pH 7.5, and substrate concentrations of 0.1 mg/ml (2 M) for T4L and 0.117 mg/ml (170 M) for Suc-Leu-Leu-Val-Tyr-AMC. To determine the appropriate concentration for Suc-Leu-Leu-Val-Tyr-AMC in these experiments, the K m value for Suc-Leu-Leu-Val-Tyr-AMC was calculated by Hanes-Woolf analysis to be 110 M. All reactions were initiated by the addition of calcium at the concentrations indicated. Oxidation studies included the addition of 100 M hydrogen peroxide or 100 M sodium hypochlorite immediately prior, unless otherwise stated, to addition of calcium. Measurement of calpain activity for the fluorescent peptide was performed as described previously (41) using the PTI Deltascan System set at an excitation wavelength of 380 nm and emission wavelength of 460 nm. For tau proteolysis and calpain autolysis, aliquots were removed at the designated times, added to a sodium dodecyl sulfate (SDS) Laemmli stop solution (42) composed of 500 mM Tris, pH 6.8, 10 mM EDTA, 10 mM EGTA, 4% SDS, 20% DTT, and 20% glycerol, incubated for 5 min in a boiling water bath, and stored at Ϫ20°C until use. Aliquots were separated on either 7.5% (tau and 80-kDa calpain I subunit) or 12.5% (30-kDa calpain I subunit) SDS-polyacrylamide gels, transferred to nitrocellulose (43), and immunoblotted with a monoclonal antibody to tau, Tau1 (44), or a monoclonal antibody to calpain I's 80-kDa (45) or 30-kDa (Chemicon) subunit. A bacterially expressed protein (21-kDa) corresponding to the natural autolytic product of the 30-kDa subunit (23) was utilized as a positive control for 30-kDa immunoreactivity. After incubation with the primary antibody, the blots were incubated with the appropriate horseradish peroxidase-conjugated secondary antibody and developed in 3,3Ј-diaminobenzidine in the presence of hydrogen peroxide or with enhanced chemiluminescence (Amersham Corp.) per manufacturer's instructions. The resulting immunoblots were quantitated using a Bio-Rad imaging densitometer (model GS-670). Data were evaluated using analysis of variance, and values were considered significantly different when p Ͻ 0.05.
Papain and Trypsin Proteolysis-Papain proteolysis of the fluorescent peptide was carried out exactly as described for calpain except a concentration of 9.0 units/ml was used. Trypsin hydrolysis of the fluorescent peptide at 30 units/ml was carried out similarly to calpain except the pH was adjusted to 7.0.

Calpain I Proteolysis at Various Calcium Concentrations-
Two previously identified calpain I substrates, the fluorescent peptide Suc-Leu-Leu-Val-Tyr-AMC (41) and the microtubuleassociated protein tau (46), were utilized to determine calpain I activity at various calcium concentrations from 0 to 2 mM. In the case of the fluorescent peptide the K m was determined to be 110 M. A concentration slightly greater than the K m value was chosen (170 M) to determine the effects of calcium concentration on proteolysis. Fig. 1A shows the change in the initial rate of calpain I proteolysis of the fluorescent peptide with increasing calcium concentration. No proteolytic activity was observed in the absence of calcium. Calpain I hydrolysis of the fluorescent peptide was observed at a concentration of calcium as low as 5 M, although the rate was very low. The initial rate of calpain I activity dramatically increased between 5 and 100 M calcium and approached maximal activity between 100 and 500 M calcium. Half-maximal activity of calpain I was calculated by Hanes-Woolf analysis to occur at 86 M calcium.
To determine the effects of calcium concentration on calpain I proteolysis of a potential physiological substrate, calpain I activity was measured using tau as a substrate. In these studies recombinant tau representing the longest human isoform (T4L) was used. Fig. 1B shows calpain I-mediated proteolysis of tau at various calcium concentrations after 30 s of incubation. Calpain I-induced degradation of tau was not detectable at 30 s until the calcium concentration was greater than 20 M. However, a profile of hydrolytic activity similar to that obtained with the fluorescent peptide was observed at calcium concentrations greater than 40 M.
Calpain I Autolysis at Various Calcium Concentrations-Because autolysis of calpain I has been correlated with activation, samples were analyzed to determine the autolytic state of calpain I at various calcium concentrations. Fig. 2 shows representative immunoblots of calpain I autolysis during calpain I-mediated proteolysis of tau at the calcium concentrations indicated. No autolysis of calpain I was detected until the calcium concentration was greater than 40 M, as determined by quantitation of the change in the ratio of the 78-and 76-kDa autolyzed forms to the intact 80-kDa form. However, proteolysis of tau, as well as the fluorescent peptide, was observed at 40 M calcium (see Fig. 1, A and B, 40 M calcium). Calpain I activity was also indicated by the degradation of calpain I fragments that are present at zero time (see Fig. 6A, 40 M. Compare at arrowhead between Ϫ and ϩ H 2 O 2 at 5 min). Rapid autolysis was observed at calcium concentrations greater than 80 M. Due to the presence of an immunoreactive band migrating at 76 kDa, which can be observed at zero time, separate experiments were carried out to determine whether this amount of 76-kDa calpain I could be responsible for proteolytic activity observed at low calcium concentrations. The amount of 76-kDa calpain I present at zero time was determined by comparing the immunoreactivity of the 76-kDa to the 80-kDa form using imaging densitometry (approximately 12% of total calpain I at zero time was 76 kDa). The calculated amount was then added as intact 80 kDa to a mixture containing the fluorescent compound, Suc-Leu-Leu-Val-Tyr-AMC, and activity was stimulated as described (see "Experimental Procedures") at 2 mM free calcium to facilitate rapid autolytic conversion to the 76-kDa form and initiate maximal proteolytic activity. However, no measurable proteolytic activity was observed at this concentration of 76-kDa calpain I. The rate of autolytic conversion of the 30-kDa subunit of calpain I was also examined at various calcium concentrations. In good agreement with previous studies (21,22), conversion of the 30-kDa subunit to 18 kDa was observed at a slower rate and at a higher minimal calcium concentration than observed for the 80-kDa subunit (data not shown).
Calpain I Autolysis at Various Substrate Concentrations-To determine whether calpain I autolysis was affected by substrate concentration, calpain I was incubated in the presence of various concentrations of the fluorescent peptide for up to 5 min at 2 mM calcium. At high substrate concentrations an inhibition of calpain I autolysis was observed (Fig. 3A). This was especially apparent at 150 M Suc-Leu-Leu-Val-Tyr-AMC, where very little calpain I autolysis occurred compared with the autolysis that occurred in the absence of substrate. In contrast, there was no apparent substrate inhibition on calpain I activity. Proteolytic activity increased up to 420 M fluorescent peptide, and activity data exhibited classical Michaelis-Menten kinetics (see Fig. 3B).
Effects of Oxidation on Calpain I Activity-To determine the effects of oxidation on calpain I-mediated proteolysis, both the fluorescent peptide and tau were proteolyzed by calpain I in the presence of 100 M hydrogen peroxide. Calpain I proteolysis of the fluorescent peptide was significantly decreased in the presence of 100 M peroxide (Fig. 4A). Although the initial rates of proteolysis of the substrate by peroxide-treated and control were not significantly different, the extent of proteolysis was significantly decreased by the presence of oxidant. Under control conditions, calpain I continued to cleave the substrate over the time course studied, whereas in the presence of peroxide the rate of substrate proteolysis by calpain I rapidly decreased and was completely inhibited after 3 min. The results were similar for all calcium concentrations between 40 M and 2 mM. In addition, the oxidant-induced inhibition of calpain I activity was immediately recoverable by addition of the reducing agent DTT (Fig. 4B). Similar experiments were carried out with tau as the substrate. Fig. 5 shows the effects of oxidation on calpain I-mediated proteolysis of tau at 40 M calcium. The presence of peroxide significantly inhibited calpain I-mediated proteolysis of tau. Results with another oxidant, 100 M sodium hypochlorite, were similar to those observed with peroxide (data not shown).
Because calpain I contains several cysteine residues throughout its structure (47), experiments were carried out in an attempt to elucidate whether the observed oxidative inhibition of calpain I activity was due to oxidation of the cysteine group of the active site or simply a generalized oxidative event. First, calpain I was preincubated with 40 or 100 M calcium, which should result in conformational changes that expose the catalytic, thiol-protease domain to the environment, in the presence or absence of 100 M peroxide for 10 min, and then added to a reaction mixture containing 0.1 mg/ml tau, resulting in a final concentration of 10 M peroxide. The reaction was stopped at time points 30 s and 50 min by addition of SDS stop, immunoblotted for tau, and the amount of tau proteolysis quantitated. Pretreatment with oxidant significantly reduced the rate of calpain I-mediated proteolysis at both calcium concentrations compared with controls (data not shown). Second, the same experiment was done except calpain I was preincubated with no calcium in the presence or absence of 100 M peroxide for 10 min. When calpain I was added to the reaction mixture containing tau as above, and then activated by calcium addition, no difference in the rate of calpain I-mediated proteolysis was observed between peroxide-treated and control samples (data not shown).
Effects of Oxidation on Calpain I Autolysis-To determine whether the reduction in activity of calpain I by oxidation was due to inhibition of autolysis of the 80-kDa to the 76-kDa form, No proteolysis was observed in the absence of calcium, and peroxide alone (no calcium present) had no effect on tau degradation (data not shown). Peroxide-treated calpain I showed a significant reduction in tau proteolysis at 40 M calcium with nearly 40% T4L remaining after 5 min compared with only 5% for control. Mean Ϯ S.E., n ϭ 3 separate experiments (*** ϭ p Ͻ 0.05). samples from fluorescent peptide and tau proteolytic experiments were analyzed for the state of calpain I autolysis. The presence of oxidant had no effect on the rate or extent of calpain I autolysis of either the 80-or 30-kDa subunits regardless of the calcium concentration (Fig. 6A). However, in the presence of oxidant, the proteolytic degradation of the 76-kDa form of calpain I was apparently inhibited (Fig. 6B).
Effects of Oxidation on Other Proteases-To determine whether the effects of oxidation on calpain I-mediated proteolysis were specific for thiol proteases, another thiol protease, papain, and a serine protease, trypsin, were examined using the fluorescent peptide as substrate. In the case of papain, the effects of peroxide were similar to those observed for calpain I, with substantial inhibition of papain activity after 2-3 min (Fig. 7A). Trypsin proteolysis, however, was unaffected by the presence of oxidant (Fig. 7B), and proteolysis of substrate continued throughout the time course studied. DISCUSSION Calpain I has been suggested to play a role in several pathogenic conditions including Alzheimer's disease (12)(13)(14)(15) and stroke (10,11). In addition, oxidative stress has been postulated to be a significant factor in these same disease states (35,38). It is important, therefore, to understand the mechanism(s) involved in calpain I activation as well as factors that modulate activity. In this study, several features of calpain I activation/ activity were defined, in vitro, including the novel finding that oxidation of calpain I results in inhibition of activity without altering autolytic conversion of either the 80-or 30-kDa subunits.
Over the past several years it has become increasingly apparent that proteolytic processing of various substrates by calpain I most likely plays a crucial role in both physiological (4) and pathological conditions (12,15,48). Because of this, it is important to understand the mechanisms of autolysis and selfproteolysis and how exogenous regulators, e.g. calcium and phospholipids, modulate calpain I activity. It was originally proposed that calpain I activity is regulated through calciummediated autolysis, a proteolytic event that cleaves the N terminus of the 80-kDa protein resulting in either the 78-or 76-kDa form (49). Studies have indicated that autolysis of calpain I to the 76-kDa form apparently lowers the calcium concentration required for activity as well as increasing specific activity (50). However, interpretation of these data has recently been questioned (19,24) as it has been suggested that it is dissociation of the heterodimer that is responsible for activation of the calpains. Regardless of these findings it is clear that autolysis to the 76-kDa form is not a prerequisite for activity because in this study and others (7,18) calcium-dependent proteolytic activity was observed in the absence of autolysis. Conflicting results regarding the necessity of calpain I autolysis for proteolytic processing are most likely due to different assay conditions. For example, in this study calpain autolysis  (top panel, arrowhead). B, immunoblots of an extended time course of calpain I autolysis at 2 mM calcium in the absence (Ϫ) or presence (ϩ) of 100 M hydrogen peroxide for the times indicated, representative of the results from three separate experiments. In the absence of peroxide calpain undergoes rapid autolysis to the 76-kDa form and is then degraded over the next several minutes and is completely proteolyzed after 30 min of incubation. In the presence of peroxide, calpain I still undergoes rapid autolysis to the 76-kDa form, but "self-proteolysis" is inhibited as the polypeptide is still present after 30 min of incubation. was significantly inhibited by high concentrations of fluorescent substrate, although proteolytic activity was unaltered. It is likely that the presence of numerous factors such as calcium, calpastatin, phospholipids, and substrates modulate both autolytic and proteolytic activity of calpain I (20). Additionally, calpain I regulation occurs through inactivation by "self-proteolysis" into inactive polypeptides. Therefore, as previously demonstrated (50,51), an initial increase in calcium concentration leads to autolysis of calpain I and the formation of an active protease, which is followed by enzyme inactivation through "self-proteolysis" which further complicates the issue.
Another important factor in calpain activation is the role of the noncatalytic 30-kDa subunit. It has been postulated that auto-conversion of the 30-kDa subunit to an 18-kDa polypeptide is involved in the activation of calpain I by lowering the calcium requirement for activity (52,53). However, more studies are required since this study and others (7,18) have shown that degradation of the 30-kDa subunit occurs subsequent to autolysis of the 80-kDa subunit, and/or at higher calcium concentrations. Recently, it has been suggested that the 30-kDa subunit acts as an inhibitor of the 80-kDa subunit and that dissociation of the heterodimer is required for calpain activation (19,24). Furthermore, it has been speculated that although autolysis is not required for activity, autolysis of the calpain subunits accelerates dissociation and through this process facilitates activation (24). Although this is an intriguing hypothesis it requires further examination, and the current study does not address this issue.
There have been numerous studies examining the autolytic and activity state of calpain I in a variety of conditions (10,12,54,55). The mechanism(s) involved in calpain activation and activity are complex and definitive interpretations of these findings are sometimes difficult. For example, to evaluate the role of calpain I in Alzheimer's disease, calpain I was assayed by homogenization of tissue followed by incubation with substrate such as casein (14). In this case, no significant difference in the activity of calpain I was found between the pathological tissue compared with control. However, because these assays were done under reduced conditions the true oxidative state of the tissue was negated, which is relevant since oxidative stress has been demonstrated in Alzheimer's disease (35). Based on the present study, oxidative state may be very important since calpain I activity was shown to be substantially decreased under oxidizing conditions. Furthermore, the present study also demonstrated that calpain I activity was recovered with addition of the reducing agent DTT, suggesting that assay of calpain I activity in the presence of a reducing agent may lead to an overestimation of in vivo calpain I activity.
Another method used to determine calpain I activity in pathological tissues is the ratio of the 76-kDa/78-kDa and 80-kDa forms of calpain I using immunoblot analysis (10,12). This type of measurement is based on the hypothesis that the 80-kDa is "pro-calpain" while the 76-kDa form is the activated enzyme. Therefore, conversion of the 80-kDa to the 76-kDa changes their ratio and is an indication of activity. Because an increase in the ratio of the 76-to 80-kDa form was found in certain pathological states compared with controls, it was hypothesized that calpain I was overactivated in the diseased tissue (12). However, based on this study, these results may need to be re-evaluated since increased levels of the 76-kDa form may not necessarily translate into increased calpain I activity under conditions of oxidative stress. Indeed, since the 76-kDa calpain I form is susceptible to "self-proteolysis" and autolysis is not inhibited by an oxidizing environment but proteolytic activity is markedly reduced, the presence of increased levels of the 76-kDa form could indicate a condition of heightened oxidative stress.
The role of calpain I during oxidative stress is important to understand for several reasons. From the present study, it is apparent that oxidative stress could result in a net decrease in calpain-mediated proteolysis, resulting in the accumulation of the autolyzed form of calpain I as well as calpain I substrates.
Because calpain I has been proposed to be involved in many physiological functions (1-4), accumulation of autolyzed calpain I may be of concern in pathological states which result in transient changes in intracellular calcium and/or redox potential such as ischemia where overactivation may lead to cell injury or cell death. The accumulation of substrate proteins may play a role in several disease states, such as Alzheimer's disease, in which the pathological condition is defined in part by the formation of paired helical filaments which are aggregates of the tau protein (57,58). The results of the present study suggest decreased activity of calpain I due to an oxidizing environment could contribute to the abnormal accumulation of tau in Alzheimer's disease. In addition, oxidizing conditions have been shown to potentiate tau self-association (59) and the formation of paired helical filament-like structures from tau constructs of just the microtubule-binding domains (60). Other substrates that could be affected by an oxidation-induced decreased calpain I activity include protein kinase C (17), calcineurin (26), and glutathione peroxidase (61), all of which can dramatically alter the ability of the cell to function properly. Fig. 8 is a diagrammatic representation summarizing the potential pathways involved in the activation and activity of calpain I as suggested by the present study. Calpain I is active only in the presence of calcium as neither proteolytic nor autolytic activity has been observed in a calcium-free environment. Increasing calcium to levels below that required for autolysis activates the 80-kDa form resulting in proteolytic activity. Activity of the 80-kDa form was also suggested by Molinari et al. (18), who demonstrated that unautolyzed calpain I in erythrocytes localized to the plasma membrane was capable of proteolyzing calcium-ATPase. If calcium concentrations rise above the level required for autolysis, which may depend on the presence and type of substrate as well as other factors (20), calpain I would convert to a 78-kDa and then to the 76-kDa form. This conversion may be inhibited by the presence of high substrate concentrations. Because proteolytic activity was unaffected by increasing substrate concentrations, a variable that inhibited calpain I autolysis, it can be suggested that, in this case, it is the extent of calcium binding which controls the activation level of calpain I, not calpain I autolysis. However, it is clear that calpain I autolysis is rapid when substrate concentration is not excessive. In cases where calpain I is activated and capable of proteolytic activity, whether autolyzed or not, the presence of an oxidant, such as hydrogen peroxide, blocks the hydrolysis of substrate. The presence of oxidant had no effect on the rate or extent of calpain I autolysis of either the 80-or 30-kDa subunits. These data indicate that it is the cysteine residue within the active site of calpain I active site that is susceptible to oxidation, since inhibition only occurred when calpain was exposed to an oxidant in the presence of calcium.
Although these data clearly demonstrate that autolysis of calpain I is not a prerequisite for proteolytic activity, the role of autolysis in calpain function remains unknown. In addition, it is also clear that oxidation can reversibly inhibit calpain proteolytic activity with no effect on autolytic conversion. Clearly, much remains to be learned in order to fully understand the mechanism(s) involved in the process of calpain I-mediated proteolysis and its role in physiological and pathological conditions. FIG. 8. Diagrammatic representation summarizing the proposed autolytic and non-autolytic calpain I proteolytic pathways as suggested by the findings of this study. The native 80-kDa form in the absence of calcium remains inactive. Upon increased calcium concentrations, calcium binds to calpain I resulting in the autolytic conversion to the 78-and 76-kDa forms, both of which have proteolytic activity. Modest calcium increases lead to calcium binding of fewer sites, resulting in a less active, but fully intact 80-kDa calpain I molecule. In cases of high calcium concentration and autolytic inhibition (e.g. in the presence of high substrate concentrations), calpain I becomes a fully active enzyme in its 80-kDa form. Although autolytic conversion is unaffected, in all cases of calpain I activity, substrate proteolysis is blocked by the presence of oxidant.