Molecular Cloning of a New Interferon-induced PML Nuclear Body-associated Protein*
- Celine Gongora‡,
- Gregory David§,
- Lionel Pintard‡,
- Catherine Tissot‡,
- The Duc Hua‡,
- Anne Dejean§ and
- Nadir Mechti‡¶
- From the ‡Institut de Genetique Moleculaire de Montpellier-UMR 9942, CNRS, 34033 Montpellier Cedex 1 and the§Unité de Recombinaison et Expression Génétique, INSERM U163, Institut Pasteur, 75724 Paris Cedex 15, France
Abstract
Transcriptional induction of genes is an essential part of the cellular response to interferons. We have established a cDNA library from human lymphoblastoid Daudi cells treated for 16 h with human α/β-interferon (IFN) and made use of differential screening to search for as yet unidentified IFN-regulated genes. In the course of this study, we have isolated a human cDNA that codes for a 20-kDa protein sharing striking homology with the product of the Xenopus laevis XPMC2 gene. This new gene is induced by both type I and II IFNs in various cell lines and will be referred to as ISG20 for interferon-stimulated gene product of 20 kDa. Confocal immunofluorescence analysis of the subcellular localization of ISG20 protein reveals that it is closely associated with PML and SP100 gene products within the large nuclear matrix-associated multiprotein complexes termed the PML nuclear bodies.
Footnotes
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↵* This work was supported by grants from the Association pour la Recherche contre le Cancer, INSERM, CNRS, the Association Nationale pour la Recherche sur le Sida, and the Federation des Centers de Lutte contre le Cancer.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBank™/EMBL Data Bank with accession number(s) .
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↵¶ To whom correspondence should be addressed: Inst. de Genetique Moleculaire de Montpellier-UMR 9942, CNRS, BP 5051, 1919 Route de Mende, 34033 Montpellier Cedex 1, France. Tel.: 4-67-61-36-61; Fax: 4-67-04-02-45.
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↵1 The abbreviations used are: IFNs, interferons; HuIFN, human interferon; PML NBs, PML nuclear bodies; HA, hemagglutinin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ORF, open reading frame.
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- Received April 1, 1997.
- Revision received May 8, 1997.
