Structure and Expression of Human Fibroblast Growth Factor-10*
- Hisayo Emoto‡,
- Shuzo Tagashira§,
- Marie-Geneviève Mattei¶,
- Masahiro Yamasaki‡,
- Gakuji Hashimoto§,
- Takashi Katsumata§,
- Takaharu Negoro§,
- Masashi Nakatsuka§,
- Daniel Birnbaum‖,
- François Coulier‖ and
- Nobuyuki Itoh‡**
- From the ‡Department of Genetic Biochemistry, Kyoto University Faculty of Pharmaceutical Sciences, Kyoto 606-01, Japan, the§Sumitomo Pharmaceutical Research Center, Osaka 554, Japan, the ¶U406 INSERM, 13385 Marseille, France, and the ‖U119 INSERM, 13009 Marseille, France
Abstract
We isolated the cDNA encoding a novel member of the human fibroblast growth factor (FGF) family from the lung. The cDNA encodes a protein of 208 amino acids with high sequence homology (95.6%) to rat FGF-10, indicating that the protein is human FGF-10. Human FGF-10 as well as rat FGF-10 has a hydrophobic amino terminus (∼40 amino acids), which may serve as a signal sequence. The apparent evolutionary relationships of human FGFs indicate that FGF-10 is closest to FGF-7. Chromosomal localization of the humanFGF-10 gene was examined by in situhybridization. The gene was found to map to the 5p12-p13 region. Human FGF-10 (amino acids 40 to 208 with a methionine residue at the amino terminus) was produced in Escherichia coli and purified from the cell lysate. Recombinant human FGF-10 (∼19 kDa) showed mitogenic activity for fetal rat keratinizing epidermal cells, but essentially no activity for NIH/3T3 cells, fibroblasts. The specificity of mitogenic activity of FGF-10 is similar to that of FGF-7 but distinct from that of bFGF. In structure and biological activity, FGF-10 is similar to FGF-7.
Footnotes
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↵* This work was supported in part by the Yamanouchi Foundation for Research on Metabolic Disorders, the Foundation of Growth Science in Japan, and grants-in-aid for scientific research from the Ministry of Education, Science and Culture of Japan.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBank™/EMBL Data Bank with accession number(s) AB002097.
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↵** To whom correspondence should be addressed: Dept. of Genetic Biochemistry, Kyoto University Faculty of Pharmaceutical Sciences, Yoshida-Shimoadachi, Sakyo, Kyoto 606-01, Japan. Fax: 81-75-753-4600; E-mail: itohnobu{at}pharm.kyoto-u.ac.jp.
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↵1 The abbreviations used are: FGF, fibroblast growth factor; PCR, polymerase chain reaction; FRSK, fetal rat keratinizing epidermal.
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- Received April 11, 1997.
- Revision received July 2, 1997.
