Identification of a 190-kDa Vascular Endothelial Growth Factor 165 Cell Surface Binding Protein on a Human Glioma Cell Line*
Abstract
Vascular endothelial growth factor (VEGF) is an angiogenesis factor for which two signaling protein tyrosine kinase receptors, Flt1 and KDR, have been identified. We describe here a 190-kDa component present on a human glioma cell line that binds VEGF165 with high affinity. In contrast, VEGF121 is bound only with low affinity, suggesting that the C-terminal part of VEGF165 is important for interaction with the 190-kDa component. No internalization or stimulation of tyrosine phosphorylation was recorded after ligand binding to the 190-kDa component, suggesting that it may not be directly involved in signaling; its function may be to present ligand or stabilize ligand binding to signaling receptors.
Footnotes
-
↵* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
-
↵‡ These authors were partly supported by a scholarship from Uehara Memorial Foundation.
-
↵§ To whom correspondence should be addressed. Tel.: 46-18-174146; Fax: 46-18-506867; E-mail: C-H.Heldin{at}LICR.uu.se.
-
↵1 The abbreviations used are: VEGF, vascular endothelial growth factor/vascular permeability factor; PAE, porcine aortic endothelial.
-
↵2 T. Omura, K. Miyazawa, A. Östman, and C.-H. Heldin, unpublished observations.
-
- Received May 15, 1997.
