Interaction of WW Domains with Hematopoietic Transcription Factor p45/NF-E2 and RNA Polymerase II*
- From the ‡Section of Molecular and Cellular Biology, University of California, Davis, California 95616, the§Department of Biochemistry, Mount Sinai School of Medicine, New York, New York 10029, and the ¶Institute of Molecular Biology, Academia Sinica, Taipei 11529, Republic of China
Abstract
NF-E2 is an erythroid-specific transcription factor required for expression of several erythroid-specific genes. By Far-Western blotting and yeast two-hybrid assay, we demonstrate that p45, the large subunit of NF-E2, is capable of binding to a specific set of WW domain-containing proteins, including the ubiquitin ligase hRPF1. This binding is mediated through the interaction between the WW domains and a PY motif located within the amino-terminal region of p45. Interestingly, the carboxyl-terminal domain of mammalian RNA polymerase II binds a similar set of WW domains to which p45 interacts with. We discuss the data in terms of possible new pathways through which the processes of transcriptional regulation by NF-E2 could be regulated in erythroid and megakaryote cells.
Footnotes
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↵* This work was supported by the United States Public Health Service, National Institutes of Health Grants DK29800 (to C.-K. J. S) and CA45757 and CA01605 (to M. S.), the National Science Council, and the National Health Research Institute, Taipei, Republic of China.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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↵‖ To whom correspondence should be addressed. Tel.: 916-752-8860; Fax: 916-752-3085; E-mail:ckshen{at}ccvax.sinica.edu.tw.
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↵1 The abbreviations used are: YAP, Yes kinase-associated protein; NEDD4, NPC-expressed, developmentally down-regulated 4; hRPF1, human receptor potentiation factor 1; GST, glutathione S-transferase; WBP1, WW domain-binding protein 1; GBD, Gal4 DNA binding domain; GAD, Gal4 activation domain; NIPP1, NF-E2-interacting polypeptide 1; CTD, COOH-terminal domain; pol, polymerase; RT-PCR, reverse transcription-polymerase chain reaction.
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↵2 N. R. Gavva, data not shown.
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↵3 N. R. Gavva and C.-K. J. Shen, unpublished data.
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↵4 X. Chen and C.-K. J. Shen, unpublished data.
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- Received July 8, 1997.
