Nucleolin Is a Protein Kinase C-ζ Substrate

We have previously shown that protein kinase C (PKC)-ζ is activated and required for nerve growth factor (NGF)-induced differentiation of rat pheochromocytoma PC12 cells (Wooten, M. W., Zhou, G., Seibenhener, M. L., and Coleman, E. S. (1994) Cell Growth & Diff. 5, 395–403; Coleman, E. S., and Wooten, M. W. (1994) J. Mol. Neurosci.5, 39–57). Here we report the characterization and identification of a 106-kDa nuclear protein as a specific substrate of PKC-ζ. NGF treatment of PC12 cells resulted in translocation of PKC-ζ and coincident phosphorylation of a protein that was localized within the nucleoplasm of nuclei isolated from PC12 cells. Addition of PKC-ζ pseudosubstrate peptide in vitro or myristoylated peptidein vivo diminished phosphorylation of pp106 in a dose-dependent fashion. Likewise, addition of purified PKC-ζ, but neither PKC-α nor δ, to nuclear extracts resulted in an incremental increase in the phosphorylation of pp106. Expression of dominant-negative PKC-ζ inhibited NGF-induced phosphorylation of pp106, by comparison overexpression of PKC-ζ enhanced basal phosphorylation without a noticeable effect upon NGF-induced effects. Amino acid sequence analysis of four peptides derived from purified pp106 revealed that this protein was homologous to nucleolin. Using anin vitro reconstitution system, purified nucleolin was likewise shown to be phosphorylated by purified PKC-ζ. The staining intensity of both enzyme and substrate in the nucleus increased upon treatment with NGF. In vivo labeling with32Pi and stimulation of PC12 cells with NGF followed by immunoprecipitation with anti-nucleolin antibody corroborated the in vitro approach documenting enhanced phosphorylation of nucleolin by NGF treatment. Taken together, the findings presented herein document that nucleolin is a target of PKC-ζ that serves to relay NGF signals from cell surface to nucleus in PC12 cells.

able membranes, and differentiation into a sympathetic neuronal phenotype (2). Several signaling molecules and second messenger systems have been identified that participate in relaying signals from the NGF receptor to the nucleus, one of which is protein kinase C (PKC).
PKC is a ubiquitously expressed serine/threonine kinase which has been implicated in a wide variety of cellular processes (3)(4)(5). PKC is a multigene family consisting of 12 structurally related isoforms which have different tissue distribution, as well as, cofactor and substrate specificities (4,5). Based upon structural features the isoforms of the PKC family can be grouped into three related groups: classical/conventional, cP-KCs (␣, ␤ I,II , and ␥) that are sensitive to calcium/diacylglycerol and tumor promoting phorbol esters; novel/atypical (␦, ⑀, , and ) that are sensitive to diacylglycerol and phorbol esters but insensitive to calcium; and atypical aPKCs ( and /) that are insensitive to all three regulators, diacylglyerol, calcium, and phorbol esters (5). The precise role and placement of PKC within the NGF signaling cascade has been unclear and controversial. Upon treatment of PC12 cells with NGF, diacylglycerol, an endogenous PKC activator, is generated (6) followed by PKC activation (7,8). PKC activators, such as phorbol esters, mimic certain biological activities of NGF in PC12 cells (9,10). In addition, NGF has been shown to stimulate translocation of PKC activity and activation of specific isoforms (11,12). Likewise, certain NGF-specific transcripts are induced in response to PKC (13). A requirement for PKC as part of the induction pathway leading to NGF-stimulated neurite out growth has also been documented (14,15). The PKC inhibitor sphingosine blocks NGF-induced neurite outgrowth in PC12 cells (14) and microinjection of PKC antibodies inhibits NGF-induced neurite outgrowth and c-fos expression (15). In contrast, however, down-regulation or removal of cellular PKC pools by chronic treatment with phorbol esters has no effect on neurite outgrowth (16) or NGF-induced early and secondary responsive gene expression (17,18). Collectively these findings document that NGF-dependent responses in PC12 cells occur through a pathway that is sphingosine-sensitive and phorbol ester-insensitive. These observations prompted us to characterize the expression of PKC isoforms in PC12 cells (12) and to further investigate the activation of these isoforms in response to NGF (19). We have shown that NGF leads to changes in all PKC isoforms (19,20). To begin to unravel the role of this multigene family in neuronal differentiation and to provide clear insight into the role of the isoforms in neurite outgrowth, we have employed a reductionist view to their study. Since removal of both cPKC and nPKC does not abrogate NGF responses, we postulated that the aPKC pathway was dominant to those regulated by either the cPKC or nPKC isoforms for differentiation. This would be consistent with the inability of phorbol esters to inhibit NGF-induced neurite outgrowth. Thus, we elected to focus on whether NGF activated the phorbol ester insensitive/atypical PKCisoform. We documented activation of atypical/PKC-in response to NGF and parallel inhibition of this isoform by sphingosine (19). Whereas, removal of PKCattenuates NGF responsiveness (20). Taken together, these findings reveal that NGF-dependent responses leading to neurite outgrowth in PC12 cells are characterized by a pathway that is sphingosine sensitive, phorbol ester-insensitive involving atypical PKC (14, 16, 19 -21).
As an extension of our previous studies, we sought to identify a substrate of PKC-that was integral to NGF responses of PC12 cells, this led to the identification of a nuclear phosphoprotein, pp106. In this report, we characterize pp106 as a specific substrate for PKC-. Purification and amino acid sequence analysis reveal that pp106 is the nuclear protein, nucleolin. These findings provide new clues for the role(s) PKC-plays in NGF signaling and further support a growing body of evidence documenting a role for this kinase in RNA processing.

EXPERIMENTAL PROCEDURES
Reagents-PC12 cells were obtained from the American Type Culture Collection (Rockville, MD). Sf9 cells and recombinant baculovirus containing the coding regions of PKC-␣, -␦, -were from Dr. G. Koch (University of Freiburg, Germany). Plasmids pRcCMVzeta and pRcC-MVzeta mut were gifts from Dr. J. Moscat (Consejo Superior de Investigaciones Cientificas-Universidad Autonoma de Madrid, Spain). Polyclonal antinucleolin antibody was a gift from Dr. Renato J. Aguilera (University of California at Los Angeles, Department of Biology).
Transfection-PC12 cells were transfected with pRcCMV-(PC12 PKC-(ϩ) ) and pRcCMV-mut (PC12 PKC-(Ϫ) ). pRcCMV-mut contains a mutant PKC-gene (this construct is the original Xenopus laevis clone) in which a point mutation in codon 275 resulting in the substitution of lysine by tryptophan renders the enzyme inactive and confers a dominant-negative phenotype (22). 50% confluent PC12 cells were washed with serum-free RPMI medium without antibiotics. 1-2 g of the plasmids and 2-20 l of Lipofectin reagent was diluted in 100 l of serum-free medium, respectively. Lipofectin reagent was a 1:1 (w/w) liposome formulation of the cationic lipid N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimmethylammonium chloride, and dioleoyl phosphatidylethanolamine in distilled water. 0.8 ml of serum-free RPMI medium was added into the Lipofectin reagent-DNA complex. The complex was mixed gently and overlaid onto the cells. After 24 h incubation at 37°C, the DNA-containing medium was replaced by normal medium containing serum and the cells were incubated for an additional 24 h at 37°C. Cells expressing transfected genes were selected in the presence of 800 g/ml neomycin (G418) as described previously (22).
Isolation of Nuclei-PC12 cells were collected with centrifugation at 1,000 ϫ g for 2 min and resuspended in 1 ml of PKC sonication buffer (20 mM Tris, pH 7.6, 50 mM 2-mercaptoethanol, 0.1 mM EDTA, 1 mM phenylmethylsulfonyl fluoride, 1 M leupeptin, 10 mM aprotinin, 100 M sodium fluoride) containing 2 mM MgCl 2 . Nuclei were isolated following a previously established procedure (21,23). In brief, cells were incubated at room temperature for 2 min and cooled in ice water for 5 min. Nonidet P-40 was added to a final concentration of 1%. After one dispersion through a 20-gauge needle, the concentration of MgCl 2 was adjusted to 5 mM. The samples were centrifuged at 600 ϫ g for 5 min. The supernatants were taken to be a mixture of cytoplasm and plasma membrane. The nuclear pellet was washed once more with PKC sonication buffer containing 5 mM MgCl 2 , resuspended in 300 l of PKC sonication buffer containing 0.1% Triton X-100, and sonicated for 10 s. Concentrations of proteins in different fractions were determined by the Bio-Rad dye binding method using bovine serum albumin as a standard. Nuclei obtained in this manner possess intact nuclear membranes and were free from significant cytoplasmic contamination (21).
Western Blotting-Western blot analysis of PKCwas carried out using 50 g of nuclear protein denatured in SDS sample buffer (125 mM Tris, pH 6.8, 20% glycerol, 1.5 M 2-mercaptoethanol, 15 mM SDS, 0.2 mg/ml bromphenol blue) and separated using a 10% SDS-polyacrylamide gel (19). The separated proteins were transferred to nitrocellulose and processed for immunoblotting with isoform-specific antisera as described previously (19,20). The relative changes in intensities were determined by densitometry (Molecular Dynamics Personal Densitometer SI, Sunnyvale, CA).
In Vitro Protein Phosphorylation-To a reaction (100 l, total volume), 25 g of nuclear protein was mixed with 65 l of pre-mix containing 17.75 mM PIPES, pH 6.5, 10 mM MgCl 2 , and 20 g/ml phosphatidylserine. In the presence or absence of 150 M PKCpseudosubstrate peptide, the assay was initiated by adding 5 l of [␥-32 P]ATP (a 1:4 mixture of 200 Ci of [␥-32 P]ATP and 750 M ATP) for 10 min at 30°C (24). Essentially the same method was used to examine phosphorylation of purified nucleolin by purified PKC-. The reaction was terminated by addition of 100 l of SDS sample buffer. Thereafter, the sample was boiled for 5 min and separated on 7.5% SDS-polyacrylamide gel electrophoresis. The gel was then stained, destained, dried, and exposed to x-ray film at Ϫ80°C. Changes in the phosphorylation state of pp106/nucleolin were determined by densitometry.
Expression and Purification of PKC-␣, -␦, --Spodoptera frugiperda (Sf9) cells (5 ϫ 10 6 ) were seeded onto 100-mm dishes and incubated in IPL-41 insect medium for 1 h at 27°C. After removing the medium, recombinant baculoviruses containing coding regions of PKC-␣, -␦, and -were added at a multiplicity of infection ϭ 10 plaque forming units/ cell and incubated with the cells for 1 h. Afterward, the inoculum was removed and 10 ml of fresh medium added. After a 4-day incubation, the cells were harvested, lysed at 4°C in PKC buffer containing (20 mM Tris, pH 7.5, 50 mM 2-mercaptoethanol, 2 mM EDTA, 100 M phentlmethylsulfonyl fluoride, 1% Nonidet P-40) followed by centrifugation at 1000 ϫ g for 15 min. The supernatant was collected and used to purify individually expressed PKC isoforms as described previously (25). The homogeneity and identity of purified PKC-␣, -␦, and -were confirmed by gel staining and Western blotting analysis using PKC isoformspecific antisera (20).
Purification of pp106 -1.3 ϫ 10 8 PC12 cells suspended in 60 ml of PBS, pH 7.3, containing 1% glucose and 1% bovine serum albumin, were treated with 100 ng/ml NGF for 5 min. Thereafter, the nuclei were isolated and used a protein for an endogenous protein phosphorylation assay. In this manner the 106-kDa protein was tagged to aid in its purification and identification during subsequent steps. The phosphorylation reaction/nuclear protein preparation was loaded on to a DEAE-Sephacel column previously equilibrated with DEAE column buffer (20 mM Tris, pH 7.5, 50 mM 2-mercaptoethanol, 2 mM EGTA, 2 mM EDTA, 100 M phenylmethylsulfonyl fluoride, 1 g/ml aprotinin, 10 g/ml leupeptin, 2.5 g/ml p-nitrophenyl phosphate, and 100 M sodium fluoride). The column was washed with DEAE column buffer containing 75 mM NaCl. The protein was eluted with DEAE column buffer using a linear gradient from 200 to 600 mM NaCl and fractions collected, an aliquot of each fraction was spotted onto filter paper and counted by Cerenkov. In addition, a separate aliquot (30 l) was analyzed by electrophoresis on a 7.5% SDS-polyacrylamide electrophoresis gel followed by staining, drying, and exposure to x-ray film. The degree of enrichment of phosphorylated pp106 and the total number of protein bands in each fraction, in comparison to the "starter" endogenous assay sample, was used to further estimate recovery and subsequent purification of pp106. The fractions containing pp106 were pooled and the conductivity adjusted to that of heparin-agarose column buffer (20 mM Tris, pH 7.6, 0.5 mM EGTA, 0.5 mM EDTA, 100 M phenylmethylsulfonyl fluoride, 1 g/ml aprotinin, 10 g/ml leupeptin, 2.5 g/ml p-nitrophenyl phosphate, and 100 M sodium fluoride). The pool was then loaded onto a heparin-agarose column, washed with column buffer containing 200 mM NaCl. Bound proteins were eluted with column buffer containing 600 mM NaCl. Fractions were collected and an aliquot was examined by SDS-polyacrylamide gel electrophoresis/autoradiography. The fractions that contained pp106 were pooled and glycerol was added to a final concentration of 1%. The pooled fractions were loaded onto a Sephacyl S-200-HR and eluded with S-200 column buffer (20 mM Tris, pH 7.5, 2 mM EGTA, 2 mM EDTA, 100 M phenylmethylsulfonyl fluoride, 1 g/ml aprotinin, 10 g/ml leupeptin, 2.5 g/ml p-nitrophenyl phosphate, and 100 M sodium fluoride). The column was developed with S-200 column buffer. The homogeneity of the purified 106-kDa protein was confirmed by the presence of a single radioactive 106-kDa protein band upon long-term exposure of the autoradiogram and a single protein band upon staining of the gel.
Microsequencing of pp106 Peptides-Purified 106-kDa nuclear protein was isolated, separated by electrophoresis on a 7.5% SDS gel, and transferred to polyvinylidene difluoride. The 106-kDa protein band was identified by staining with Ponceau-S and excised, subjected to reduction, alkylation, and Lys-C digestion. The peptides were separated by reverse phase HPLC and sequenced by automatic Edman degradation. A Swiss-Prot data base search was conducted for similarity between the derived sequences of the isolated peptides and rat nucleolin.
Immunofluorescence Microscopy-PC12 cells were treated with NGF (50 ng/ml) for 0 -30 min (acute). Thereafter, the cells were placed on ice and washed with cold PBS, followed by isolation of nuclei as described previously (20,23). The isolated nuclei were resuspended in PBS and allowed to sediment onto polylysine-coated glass slides for 15 min and fixed in 4% (v/v) paraformaldehyde for 20 min. The nuclei were permeabilized by incubation in 80% methanol in PBS for 60 min at Ϫ20°C. Alternatively, localization was monitored in cells that were plated directly onto coverslips that had been coated with a mixture of collagen/ polylysine (4:1, v/v) and treated with NGF (50 ng/ml for 3 days). The coverslips were rinsed with PBS and incubated for 3 min in 2% (v/v) paraformaldehyde in PBS and then incubated for another 3 min in 4% (v/v) paraformaldehyde in PBS. Fixed nuclei or cells were blocked in PBS containing 1% bovine serum albumin and 0.1% (v/v) saponin for 2 h at 27°C. Thereafter, polyclonal primary antibody either anti-PKC-or anti-nucleolin (1:250) was added in blocking buffer overnight and incubated at 4°C. The coverslips were rinsed three times, 5 min each followed by addition of goat anti-rabbit IgG-fluorescein isothiocyanateconjugated antibody (12 g/ml) in blocking buffer for 2 h in the dark at 27°C. Thereafter, the coverslips were mounted in glycerol/PBS and observed using a Nikon Optiphot epifluorescence microscope. As control, samples were processed without primary antibodies, or in the case of PKC with antibody that had been previously preincubated with peptide antigen. In either case, no background fluorescence could be detected.
In Vivo Labeling and Immunoprecipitation of Nucleolin/pp106 -In brief, PC12 cells were labeled overnight in growth media which had been diluted by half with serum-free/phosphate-free RPMI 1640 containing 100 Ci/ml 32 P. The cells were simulated with NGF and washed in ice-cold PBS, lysed in 1 ml of immunoprecipitation buffer (10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 1% Triton X-100, 1.5 mM MgCl 2 , 1 mM EGTA, 1 mM NaVO 3 , 10 mM sodium fluoride, 1 mM phenylmethylsulfonyl fluoride, 1 mM aprotinin, 30 M microcysin, and 1 M leupeptin). The extracts were treated with DNase and RNase, followed by immunoprecipitation as described previously using a 1:500 dilution of anti-nucleolin antisera (26). The protein A-Sepharose beads were washed in immunoprecipitation buffer containing 0.05% deoxycholate. The beads were resuspended in Laemmli sample buffer and electrophoresed on a 7.5% SDS-polyacrylamide gel. The gel was stained, dried, and exposed to x-ray film for 1-3 days at Ϫ80°C with intensifying screens.

NGF-Induced Phosphorylation of a 106-kDa Nuclear Protein
and Translocation of PKC--We initiated our search for substrates of PKC-that might play a role in NGF signaling by examining nuclear lysates prepared from PC12 cells for endogenously phosphorylated proteins. Under conditions which favored PKC-activation (24), we observed that NGF treatment of PC12 cells resulted in enhanced phosphorylation of a 106-kDa nuclear protein, reaching a maximum at 5 min post-treatment with NGF (Fig. 1A). Phosphorylation of pp106 was NGF dose-dependent in the range of 0 -200 ng/ml, with 100 ng/ml being optimal. We observed that phosphorylation of pp106 was enhanced when cells were cultured at low cell density, and to lesser extent, in subconfluent cultures and almost absent in cells cultured at high cell density. In parallel, samples were Western blotted with PKC-antibodies. An NGF-dependent increase in immunoreactivity of PKC- (Fig. 1B) was likewise observed in nuclei. Activity changes in nuclear PKC-were monitored by phosphorylation of ⑀-peptide, ERMRPRKRQGS-VRRRV, a synthetic peptide corresponding to amino acids 149 -164 of PKC-⑀ pseudosubstrate motif substituting Ser for Ala 159 (19,27). NGF treatment resulted in a 37% increase in nuclear PKC-activity, as well as, enzyme translocation from the cytoplasm as well as transport of enzyme into the nucleus as observed by immunoelectron microscopy (12). To examine whether NGF could directly mediate phosphorylation of pp106 at the nuclear level, isolated nuclei were directly stimulated with NGF: no change in the phosphorylation of the 106-kDa protein was observed (data not shown). To examine the localization of pp106 and PKC-within the nucleus, nuclei were fractionated into envelope and nucleoplasmic fractions. Endogenous phosphorylation analysis revealed that phosphorylated

pp106/Nucleolin Is a PKC-Substrate
pp106 was restricted to nucleus and localized within the nucleoplasm ( Fig. 2A). PKC-itself was likewise enriched in the nucleoplasm post-NGF treatment (Fig. 2B). Thus, we hypothesized that pp106 might be a direct substrate of PKC-, since it was phosphorylated in a manner that was concomitant with the translocation kinetics exhibited by PKC- (Fig. 1, A and B) and hence was chosen as a candidate protein for further study.
NGF-induced Phosphorylation of pp106 Is Mediated by PKC--We evaluated the specificity of pp106 to serve as a substrate of PKC-by examining the effect which inclusion of PKCpseudosubstrate peptide had upon on NGF-induced phosphorylation of pp106 both in vivo and in vitro. PKC-pseudosubstrate peptide is a synthetic peptide corresponding to amino acids 113-129 of PKC-regulatory subunit (SIYRRGAR-RWRKLYRAN), which is homologous between all members of the aytpical PKC family. The pseudosubstrate motif suppresses PKC activity by interacting with the substrate-binding pocket in the catalytic domain (28) and can be used as a specific PKC inhibitor (8). Addition of PKC-pseudosubstrate peptide into the endogenous protein phosphorylation assay diminished phosphorylation of pp106 in a dose-dependent manner in the range of 5-200 M (Fig. 3A). By comparison, addition of pseudosubstrate peptide 19 -36, which inhibits classical PKC isoforms (␣, ␤, ␥) did not significantly diminish NGF-stimulated phosphorylation of pp106. Likewise, pretreatment of the cells with myristoylated PKC-pseudosubstrate peptide, which can be efficiently transported across the cell membrane (29), inhibited phosphorylation of pp106. In contrast, cPKC 19 -36 myristoylated pseudosubstrate peptide, corresponding to the conserved pseudosubstrate motif of PKC-␣, -␤, or -␥ had no inhibitory effect on phosphorylation of pp106 either in vitro or in vivo (data not shown). We next examined whether addition of purified PKC-directly to nuclear extracts would support phosphorylation of pp106. Addition of PKC-likewise resulted in a dose-dependent increase in the phosphorylation of pp106 (Fig. 3B), by comparison no significant change in the phosphorylation state of pp106 was observed following addition of ei-ther purified PKC-␣ or PKC-␦ isoforms. Thus, the 106-kDa nuclear protein appears to be a preferred substrate of atypical PKC-compared with either classical or nonclassical PKC.
Overexpression of a mutant PKC-, which rendered the dominant-negative phenotype, was used as another approach to confirm the functional involvement of PKC-in NGF-induced pp106 phosphorylation. pRcCMV mut construct contains a point mutation in codon 275 resulting in substitution of lysine by tryptophan rendering the enzyme inactive (22). By competing with native PKC-, dominant-negative PKC-inhibited NGF-induced phosphorylation of the 106-kDa nuclear protein.
By comparison, overexpression of PKC-significantly enhanced basal phosphorylation of pp106 which was further enhanced upon addition of NGF (Fig. 4). Similar patterns of phosphorylation were observed independent of atypical PKC gene constructs employed (X. laevis-PKC-, mouse/rat PKC-, or human PKC-). 2 Taken together, these experiments establish pp106 as a likely nuclear substrate of atypical PKC.
pp106 Is Homologous with Nucleolin-A wide spectrum of intracellular proteins have been shown to undergo phosphorylation and dephosphorylation following NGF treatment in PC12 cells (30 -32). Several proteins with an approximate mass of 100 kDa likewise exhibit altered phosphorylation states in response to NGF. Proteins such as Nsp 100 (33), calmodulinbinding protein (CaM-BP 100 ) (34), and two other phosphatidylinositol 3-kinase-associated proteins, 100 and 110 kDa (35), are examples. However, phosphorylation of Nsp 100 is inhibited by NGF (33) which is in contrast to our observations regarding pp106. NGF-induced phosphorylation of our 106-kDa protein was independent of Ca 2ϩ /calmodulin (data not shown), whereas CaM-BP 100 underwent phosphorylation in a Ca 2ϩ / calmodulin-dependent fashion (34). Phosphatidylinositol 3-kinase-associated 100-and 110-kDa proteins are reported to be tyrosine phosphorylated (35), however, we excluded these proteins since pp106 was found to be phosphorylated on serine following NGF treatment (data not shown). We also determined that pp106 was neither PKC-(105 kDa), topoisomerase I (100 kDa), retinoblastoma gene product (107 kDa), 110 kDa units of phosphatidylinositol 3-kinase, nor PLC ␤I (100 kDa), since pp106 was not immunoprecipitated by antibodies against any of these proteins (data not shown). Thus, the 106-kDa nuclear protein appeared to be both a novel NGF-regulated protein, as well as, a newly described PKC isoform-specific substrate.
Based on apparent molecular weight, nuclear localization, and phosphorylation state, we purified pp106 to homogeneity. Interestingly, the purification of pp106 was coincident with the purification scheme employed for PKC- (27). The purified protein was blotted to polyvinyldifluoride membrane, followed by Lys-C digestion. Four peptides obtained from reverse phase HPLC separation of the digested products were sequenced by automatic Edman degradation (Fig. 5). A Swiss-Prot data base search for similarity revealed the amino acid sequence of each peptide displayed complete homology with that of rat nucleolin, a major nuclear phosphoprotein with apparent mass of 105-110 kDa.
We next set up an in vitro reconstitution assay to examine whether purified nucleolin could be phosphorylated directly by purified PKC-. Increasing concentrations of PKC-lead to increased phosphorylation of nucleolin (Fig. 6A). Phosphorylation of nucleolin was not observed with preparations of either PKC-␣ or PKC-␦ isoforms (data not shown), which was consistent with previous findings (Fig. 3). Phosphoamino acid analysis revealed Ser phosphorylation of either pp106 or nucleolin by PKC- (Fig. 6B). Immunofluorescence was used to examine the localization of both kinase and substrate. To enhance detection, we examined the translocation utilizing intact nuclei rather than whole cells. Nuclei isolated employing this method are pure retaining an intact nuclear membrane (21,23). Temporal kinetics at early time points (0 -30 min) revealed an increase in PKC-immunofluorescence within the nucleus (Fig. 7A-D), in parallel, increases in nucleolin staining were likewise observed (Fig. 7E-H). By 30 min both nucleolin and PKC-staining were concentrated in the nuclear membrane. During longer term treatment (3 days) both nucleolin and PKC-colocalized to the perinuclear region of PC12 cells (Fig. 8, A and B).
To provide further support that nucleolin/pp106 was a PKCsubstrate whose phosphorylation is mediated by NGF, the findings were corroborated by an alternate set of experiments. PC12 cells were labeled overnight with orthophosphate followed by NGF stimulation and immunoprecipitation with antinucleolin antibody (26). We observed enhanced phosphorylation of pp106/nucleolin in vivo reaching a maximum at 5 min post-NGF treatment (Fig. 9A). The phosphorylation of nucleolin was likewise dependent upon the dose of NGF (Fig. 9B). In summary, we documented the following: 1) nuclear translocation of PKC-concomitant with phosphorylation of the 106-kDa nuclear protein; 2) direct phosphorylation of pp106 by PKC-and not other PKC isoforms; 3) inhibition of pp106 phosphorylation by atypical pseudosubstrate both in vitro and in vivo; 4) enhanced basal phosphorylation of pp106 by overexpression of PKC-; 5) phosphorylation of purified nucleolin/ pp106 by purified PKC-; 6) copurification, as well as, localization of both nucleolin and PKC-to similar sites within the cell; and last, 7) NGF-induced phosphorylation of pp106 in vivo by immunoprecipitation with anti-nucleolin antibody paralleled the findings in vitro. Collectively, these findings demonstrate that pp106 is the nuclear protein nucleolin which serves as a substrate for PKC-and connects NGF cell surface signaling with the nucleus.

DISCUSSION
Atypical PKC-has been implicated in a variety of cellular functions such as maturation of X. laevis oocytes (36), proliferation of mouse fibroblasts (37), maintenance of long term potentiation (38), brain development (39), insulin-induced glucose transport (40), platelet-derived growth factor-stimulated stromelysin gene expression (41) pp106/Nucleolin Is a PKC-Substrate lation of COX transcription (44), NGF responses (21), and more recently PKC-has been shown to play a role in cell death (45).
Nucleolin is a major constituent of nucleoli in exponentially growing cells (46) and functions in the organization of nucleolar chromatin (47), packaging of pre-rRNA (48), rDNA transcription (49), and ribosome assembly by shuttling between the nucleus and the cytoplasm (50). In addition, nucleolin has been reported to serve as substrate for casein kinase II during interphase of the cell cycle (51,52) and for the cell cycle-regulatory cdc2 kinase during mitosis (53,54). Nucleolin shuttles from nucleus to cytoplasm, however, we observed that only the protein localized within the nucleus is phosphorylated by PKC-. It is possible that the protein assumes an alternate conformation upon exit from the nucleus which masks the phosphorylation site. Interestingly, phosphorylation of nucleolin has been shown to regulate its helicase activity (55) and thus, phosphorylation by PKC-, as well as other kinases may likely regulate its functional abilities in chromatin organization, rRNA packaging, rDNA transcription, or ribosome assembly. The translocation of PKC-itself may be directed by a bipartite nuclear targeting motif within the enzyme (20) or proteins which facilitate import into the nucleus. Although, fibroblast growth factor-2 has been shown to directly stimulate phosphorylation of nucleolin through a casein kinase II-mediated pathway directly at the nuclear membrane (56), we failed to observe enhanced phosphorylation of nucleolin by NGF under similar conditions. Thus, this observation further underscores the differences in the two growth factors and suggests that NGF mediates movement of PKC-into the nucleus by a pathway that originates at the plasma membrane. Alternatively, the local concentration of second messenger within the vicinity of the nucleus may specifically activate PKC-within that particular microenvironment. In this regard, PKC-is activated by phosphatidic acid, which is generated by the activation of phospholipase D that is also localized to the nuclear membrane (57,58) and may play a role as a second messenger.
Interestingly, overexpression of X. laevis PKC-resulted in a somewhat unexpected finding. Although enhanced phosphorylation of pp106 was observed by introduction of PKC-construct, overexpression itself did not result in a dramatic increase in NGF-stimulated phosphorylation levels. To rule out any possible differences in gene constructs we have employed a critical study of various genes of the atypical family. 3 The X. laevis gene originally cloned by Moscat and colleagues (36) displays 90% homology with the human PKC-cloned by Selbie et al. (59). PKC-is likewise homologous to the mouse PKC-. Using various constructs of atypical PKCs (X. laevis PKC- (36), mouse PKC-(60), and human PKC-(59)) we have found no differences in NGF-mediated responses in the phosphorylation pattern to nucleolin. 3 Both genes, /, and , are highly homologous encoding proteins with similar functional properties. A growing body of data suggests that signaling pathways likely maintain their differential specificity depending upon the cellular background in which they are expressed. Thus, it is possible in cells other than PC12 that atypical PKCs-/ andmay possess differential modes of regulation or that nucleolin may be phosphorylated in a differential manner. Our overexpression studies therefore suggest that atypical PKC may interact with other upstream signaling pathways since the basal phosphorylation state of the downstream target, nucleolin, was enhanced. The findings presented herein, along with other studies (12,(61)(62)(63), further implicates a role for this PKC-in mediating nuclear responses. Interestingly, PKC-accumu-lates within the perinuclear membrane during longer term treatment with NGF and thus, may play a role in signals necessary for longer term morphological differentiation.
Nucleolin has been shown to preferentially interact with some specific regions of DNA (64,65) with the nonphosphorylated form serving as a negative regulator of transcription (46,48), perhaps by altering the topography of DNA (55). Nucleolin also binds to the 3Ј-untranslated region of amyloid protein precursor (APP) mRNA (66). Thus, a possible role for PKC-is to modulate nucleolin-RNA interactions. In this regard, hnRNP A1 has been shown to serve as a substrate of PKC-(67); phosphorylation impairs hnRNPA1 RNA binding and its ability to promote strand annealing. Both hnRNP A1 and -C share similar properties in their ability to bind reiterated AUUUA sequences (68). We speculate that PKC-may regulate the formation of a complex formation between hnRNP A1 and nucleolin and thus affects it ability to bind certain mRNAs, such as APP. Additionally, the APP gene itself may be directly regulated by PKC-. Previous findings have shown that ␤ promoter activation to be dependent upon PKC- (42). APP has both a ␤ regulatory site (69) and is also regulated by NGF (70). Thus, APP may serve as an example of a gene whose expression is regulated by the "double-control" model (67) subject to coordinate regulation both at the transcriptional and translational levels by PKC-.
Numerous questions remain to be answered such as the signaling route taken to mediate this response; the physiological second messenger signal cascade that drives this response; the relationship of NGF-receptor components in this process; the effect of PKC-mediated phosphorylation on shuttling of nucleolin; and effects of differential phosphorylation by cdc2, casein kinase II, and PKC-on RNA binding and helicase activity. Obviously there is a great deal more work that is needed to address these questions.