Pombe Cdc5-related Protein
A PUTATIVE HUMAN TRANSCRIPTION FACTOR IMPLICATED IN MITOGEN-ACTIVATED SIGNALING*
- From the ‡ Cardiovascular Research Institute,
- Departments of § Pediatrics and
- ∥ Medicine, University of California, San Francisco, California 94143
- ** To whom correspondence should be addressed: UCSF, Box 0130, San Francisco, CA 94143-0130. E-mail: shaun_coughlin.src{at}quickmail.ucsf.edu
Abstract
The Schizosaccharomyces pombe cdc5 gene product is a cell cycle regulator that exerts its effects at the G2/M transition in fission yeast. We describe the cloning of a putative human transcription factor, pombe Cdc5-related protein (PCDC5RP), which bears significant homology to S. pombe Cdc5 and to expressed sequences in mouse, nematode, and budding yeast. PCDC5RP is expressed widely in normal adult human tissues and thus may have an important general function that has been preserved evolutionarily. PCDC5RP contains two tandem repeats of a helix-turn-helix DNA binding motif, four consensus nuclear localization signals, and a hydrophilic, proline-rich central region similar to the transcriptional activating domain in Myb family members. Remarkably, PCDC5RP moved rapidly from cytoplasm to nucleus upon serum stimulation of cultured cells. This movement correlated temporally with an increase in PCDC5RP phosphorylation. Thus, PCDC5RP is a presumed transcription factor that appears to transduce cytoplasmic signals to the nucleus upon serum stimulation.
Footnotes
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↵¶ Supported by Clinical Investigator Development Award HL03228 from the National Institutes of Health.
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↵* This work was supported in part by National Institutes of Health Grant HL44907. The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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↵1 The abbreviations used are:
- PCDC5RP
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pombe Cdc5-related protein
- kb
-
kilobase(s)
- DMEM
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Dulbecco's modified Eagle's medium
- MAP
-
mitogen-activated protein.
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- Received October 18, 1996.
- Revision received December 10, 1996.
- © 1997 by The American Society for Biochemistry and Molecular Biology, Inc.
