Interaction of Drosophila Inhibitors of Apoptosis with Thick Veins, a Type I Serine/Threonine Kinase Receptor for Decapentaplegic*
- From the ‡Department of Biochemistry, The Cancer Institute, Tokyo, Japanese Foundation for Cancer Research and Research for the Future Program, Japan Society for the Promotion of Science, 1-37-1 Kami-Ikebukuro, Toshima-ku, Tokyo 170-8455, Japan and the§Third Department of Internal Medicine, Yamaguchi University School of Medicine, 1144 Kogushi, Ube, Yamaguchi 755-8505,Japan
Abstract
Decapentaplegic (Dpp) is a Drosophilamember of bone morphogenetic proteins, which belong to the transforming growth factor-β superfamily. Members of this family regulate a variety of biological processes such as cell proliferation, morphogenesis, immune response, and apoptosis. Dpp plays a critical role in many aspects of Drosophila development. Members of the transforming growth factor-β superfamily bind to two different types of serine/threonine kinase receptors, termed type I and type II. Type I receptors act as downstream components of type II receptors in the receptor complexes. Therefore, intracellular proteins that interact with the type I receptors are likely to play important roles in signaling. Several proteins have been identified through protein-protein interaction screenings. We identifiedDrosophila inhibitor of apoptosis (DIAP) 1 as an interacting protein of a Dpp type I receptor, Thick veins (Tkv). DIAP1 associates with Tkv in vivo. The binding region in DIAP1 is mapped to its C-terminal RING finger region. DIAP2, anotherDrosophila member of the inhibitor of apoptosis protein family, also interacts with Tkv in vivo. These data suggest that DIAP1 and DIAP2 may be involved, possibly as negative regulators, in the Dpp signaling pathway, which leads to cell apoptosis.
Footnotes
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↵* This work was supported by grants-in-aid for scientific research from the Ministry of Education, Science and Culture of Japan and special coordination funds for promoting science and technology from the Science and Technology Agency.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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↵¶ Supported by the Toray Scientific Foundation and Uehara Memorial Foundation.
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↵‖ To whom correspondence should be addressed. Tel.: 81-3-5394-3866; Fax: 81-3-3918-0342; E-mail:mkawabat-ind{at}umin.u-tokyo.ac.jp.
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↵1 The abbreviations used are: TGF, transforming growth factor; BMP, bone morphogenetic protein; TβR, TGF-β receptor; IAP, inhibitor of apoptosis; DIAP, Drosophila IAP; BIR, baculovirus IAP repeat; ICE, interleukin-1β converting enzyme; PCR, polymerase chain reaction; PAGE, polyacrylamide gel electrophoresis; HA, hemagglutinin.
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- Received January 21, 1998.
- Revision received February 24, 1998.
- The American Society for Biochemistry and Molecular Biology, Inc.
