The Basolateral Localization Signal of the Follicle-stimulating Hormone Receptor*
- Isabelle Beau‡,
- Marie-Thérèse Groyer-Picard‡,
- André Le Bivic§,
- Brigitte Vannier‡,
- Hugues Loosfelt‡,
- Edwin Milgrom‡ and
- Micheline Misrahi‡¶
- From ‡INSERM Unité 135, Hormones Gènes et Reproduction, Institut Fédératif de Recherche 21, Hôpital Bicêtre, Assistance Publique Hopitaux de Paris, 94275 Le Kremlin Bicêtre, France and §Laboratoire de Génétique et Physiologie du Développement, Unité Mixte de Recherche 9943, Faculté des Sciences Luminy, 13288 Marseille, France
Abstract
The follicle-stimulating hormone receptor (FSHR) is physiologically localized in the basolateral compartment of the membrane of Sertoli cells. This localization is also observed when the receptor is experimentally expressed in Madin-Darby canine kidney cells. We thus used in vitro mutagenesis and transfection into these polarized cells to delineate the basolateral localization signal of the receptor.
The signal was localized in the C-terminal tail of the intracellular domain (amino acids 678–691) at a marked distance of the membrane. Mutation of individual amino acids highlighted the importance of Tyr684 and Leu689. The 14-amino acid sequence was grafted onto the p75 neurotrophin receptor and redirected this apical protein to the basolateral cell membrane compartment. Deletion of amino acids 677–695 did not modify the internalization of the FSHR, showing that the basolateral localization signal of the FSHR is not colinear with its internalization signal.
Footnotes
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↵* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
This work was supported by INSERM, the Faculté de Médecine Paris Sud, the Assistance Publique-Hôpitaux de Paris, the Association pour la Recherche sur le Cancer, and the Association de Recherches Scientifiques Paul Neumann.
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↵¶ To whom correspondence should be addressed: Hôpital de Bicêtre, INSERM U.135, 3ème niveau, 78 Rue du Général Leclerc, 94275 Le Kremlin Bicêtre, France. Tel.: 33-1-45-21-33-29; Fax: 33-1-45-21-38-22.
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↵1 The abbreviations used are: FSH, follicle-stimulating hormone; LH, luteinizing hormone; TSH, thyrotrophin; FSHR, follicle-stimulating hormone receptor; MDCK, Madin-Darby canine kidney; PBS, phosphate-buffered saline; BSA, bovine serum albumin; NTR, neurotrophin receptor; NTRt, truncated neurotrophin receptor; DMEM, Dulbecco’s modified Eagle’s medium.
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↵2 C. Baratti-Elbaz, N. Ghinea, H. Loosfelt, C. Pichon, and E. Milgrom, unpublished results.
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- Received April 9, 1998.
- The American Society for Biochemistry and Molecular Biology, Inc.
