Transcriptional Factor Mutations Reveal Regulatory Complexities of Heat Shock and Newly Identified Stress Genes in Saccharomyces cerevisiae*
Abstract
A computer-aided pattern search of the entire yeast genome was designed and used to identify 186 putative stress response element-regulated genes in Saccharomyces cerevisiae. Transcript levels of eight of these candidate genes were examined, and three (37%) were shown to be heat shock- and DNA damage-inducible and to require the Msn2p and Msn4p transcriptional activators for stress regulation. Significantly, several heat shock protein (HSP) genes were identified in this computer search. Using a series of single and multiple regulatory mutants, we demonstrate unexpected regulatory complexities among the HSP genes from S. cerevisiae following heat shock.
Footnotes
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↵* This work was supported by Research Grant GM38456 from the National Institutes of Health (to K. M.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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↵‡ Present address: Dept. of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208.
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↵§ Present address: Dept. of Medicine, Division of Hematology, University of Utah Health Sciences Center, Salt Lake City, Utah 84312
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↵¶ To whom correspondence should be addressed. Tel.: 310-825-5251; Fax: 310-206-5272; E-mail:mcentee{at}mednet.ucla.edu.
- STRE
- stress response element
- HS
- heat shock
- HSE
- heat shock element
- HSF
- heat shock transcription
- HSP
- heat shock protein
- MMS
- methyl methanesulfonate
- PDS
- post-diauxic shift
- kb
- kilobase pair(s)
- bp
- base pair(s).
- Received February 23, 1998.
- Revision received August 6, 1998.
- The American Society for Biochemistry and Molecular Biology, Inc.
