Biochemical and functional characterization of DNA complexes capable of targeting genes to hepatocytes via the asialoglycoprotein receptor.

Page 7404, Fig. 6: Panels Cand D of the original Fig. 6 were incorrect in that the reported DNA complexes were prepared from a plasmid containing a chimeric gene composed of the P-enolpyruvate carboxykinase promoter (−460 to +73) linked to the cDNA for human factor IX instead of the SV40-luciferase gene. Here we provide a revised Fig. 6 that includes an entirely new set of panels using SV40-luciferase DNA throughout.

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Figure 6

A, uncomplexed DNA; B, DNA condensed by the method of direct mixing resulting in ψ-form DNA;C, DNA complex prepared at a suboptimal concentration of NaCl (aggregated DNA complex); D, DNA complex prepared at an optimal concentration of NaCl (DNA complex condensed); E, DNA complex prepared at an above optimal concentration of NaCl (DNA complex relaxed). The bar equals 166.6 nm. See “Experimental Procedures” for further details.

This change does not alter any of the scientific conclusions of the paper.

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