Integrin-associated Protein Is a Ligand for the P84 Neural Adhesion Molecule*
- From the ‡Department of Neurobiology and the§Department of Pediatrics, University of Pittsburgh School of Medicine and ¶The Children’s Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213
Abstract
P84 (also known as SHPS-1, BIT, and SIRP) is a heterophilic adhesive membrane protein involved in receptor tyrosine kinase signaling that is found at synapses in the mammalian central nervous system and in non-neural tissues. We have identified a binding partner for P84 using an expression cloning strategy. Here we report that integrin-associated protein (IAP/CD47) is a predominant binding partner of P84. Immunohistochemistry reveals a virtually identical distribution of P84 and IAP in a variety of adult brain regions. Because IAP has been implicated in cell signaling in cells of the immune system, P84 and IAP represent a heterophilic binding pair that is likely to be involved in bi-directional signaling at the synapse and in other tissues.
Footnotes
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↵* This work was supported by a grant from the National Institutes of Health (to C. F. L. and V. N.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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↵‖ To whom correspondence should be addressed: Rm. 7151 Rangos Bldg., Children’s Hospital of Pittsburgh, 3705 Fifth Ave. at DeSoto St., Pittsburgh, PA 15213. Tel.: 412-692-6078; Fax: 412-692-7824; E-mail: nara+{at}pitt.edu.
- Abbreviations:
- CNS
-
central nervous system
- IAP
-
integrin-associated protein (CD47)
- BCIP
-
5-bromo-4-chloro-3-indolylphosphate p-toluidine salt
- NBT
-
nitro blue tetrazolium chloride
- AP
-
alkaline phosphatase
- RAP
-
receptor alkaline phosphatase
- PBS
-
phosphate-buffered saline
- BSA
-
bovine serum albumin
- FITC
-
fluorescein isothiocyanate
- N-CAM
-
neural cell adhesion molecule.
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- Received September 25, 1998.
- Revision received November 3, 1998.
- The American Society for Biochemistry and Molecular Biology, Inc.
