Expression of the H Type 1 Blood Group Antigen during Enterocytic Differentiation of Caco-2 Cells*
- From the Division of Biochemistry and Nutrition, Research Institute, International Medical Center of Japan, Tokyo 162-8655, Japan
Abstract
We made a comparative study of the structures of the oligosaccharides on the glycoproteins from Caco-2 human colonic adenocarcinoma cells, before and after differentiation. Enterocytic differentiated Caco-2 cells highly express H type 1 blood group antigen on the cell surface as well as activities of brush border membrane hydrolases, such as dipeptidyl peptidase IV and alkaline phosphatase. A strong correlation was observed between the amounts of H type 1 blood group antigen and the degrees of differentiation. Structural analysis with use of lectin affinity high performance liquid chromatography revealed that typical mucin-type sugar chains of the glycoproteins from undifferentiated cells have H type 2 group, linear polylactosamines, and core 1 structure. On the other hand, differentiated cells newly contain H type 1 and Leb groups and core 2 structure. Mucins with H type 1 group make contact with brush border membrane enzymes on differentiated cells. Furthermore benzyl 2-acetamide-2-deoxy-α-d-galactopyranoside inhibited both expression of H type 1 group on the cell surface and enhancement of brush border membrane enzyme activities even in the presence of a differentiating inducer. These results suggest that the mucin-type sugar chains with H type 1 group have important functions regarding differentiation of Caco-2 cells.
Footnotes
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↵* This work was supported by a Sasakawa scientific research grant from the Japan Science Society.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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↵‡ To whom correspondence should be addressed. Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Tel.: 81-3-3812-2111; Fax: 81-3-5841-4879.
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↵2 J. Amano, H. Morita, and M. Oshima, manuscript in preparation.
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↵3 S. Takizawa, J. Amano, and M. Oshima, unpublished data.
- Abbreviations:
- HPLC
-
high performance liquid chromatography
- AAL
-
A. aurentia lectin
- PVL
-
P. velutina lectin
- GalNAc-α-O-benzyl
-
benzyl 2-acetamide-2-deoxy-α-d-galactopyranoside
- RCA120
-
R. communis agglutinin 120
- UEA-I
-
U. europaeus lectin-I
- FITC
-
fluorescein isothiocyanate
- R-PE
-
R-phycoerythrin
- ABEE
-
aminobenzoyl ethyl ester
- DPP-IV
-
dipeptidyl peptidase-IV
- BSA
-
bovine serum albumin
- PBS
-
phosphate-buffered saline
-
- Received December 11, 1998.
- Revision received April 27, 1999.
- The American Society for Biochemistry and Molecular Biology, Inc.
