Identification of a Nuclear Respiratory Factor-1 Binding Site within the Core Promoter of the human polio virus receptor/CD155 Gene*
- From the ‡Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, New York 11794 and¶Max-Delbrück-Centrum für Molekulare Medizin, Robert-Rössle-Straβe 10, 13092 Berlin, Germany
Abstract
In this report we describe acis-acting element within the core promoter of theCD155 gene specifying the polio virus receptor that is bound by the nuclear respiratory factor-1 (NRF-1) transcription factor. DNase I footprint analysis identified a nuclear protein binding site from −282 to −264 nucleotides upstream of the translation initiation codon of the CD155 gene, which we have called foot print IV (FPIV). Linker scanning mutagenesis revealed that a tandem repeat motif, GCGCAGGCGCAG, located within FPIV was essential for the basal activity of the CD155 core promoter. The results of the electrophoretic mobility shift assay experiments suggested that identical FPIV binding activities were present in a variety of nuclear extracts and that the tandem repeat was essential for binding. A one-hybrid screen was then carried out using FPIV as bait to clone the cDNA of the FPIV binding factor. The sequences of the cDNAs that were cloned from the screen were identical to NRF-1, a result that was confirmed by further electrophoretic mobility shift assay experiments. Overexpression of full-length NRF-1 and a dominant-negative form of NRF-1 modulated reporter gene expression driven by the core promoter. Remarkably, CD155 is the first gene shown to be regulated by NRF-1 that possesses an expression profile during embryogenesis correlating with this factor's proposed role in the development of the vertebrate optic system. We propose that NRF-1, which has been shown by others to be expressed during embryogenesis in animal systems, may be involved in regulating the expression of CD155 at specific stages of central nervous system development.
Footnotes
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↵* This work was supported in part by National Institutes of Health Grant AI39485.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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↵§ Member of the graduate program in Molecular and Cellular Biology, SUNY at Stony Brook and recipient a grant from the Deutscher Akademischer Austauschdienst. To whom correspondence should be addressed: Laboratory of Developmental Neurobiology, The Rockefeller University, New York, NY 10021. Tel.: 212-327-7211; Fax: 212-327-7140; E-mail:soleckd@rockvax.rockefeller.edu.
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↵‖ Supported by Grant BE1886/1-2 from the Deutsche Forschungsgemeinschaft.
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↵1 M. Gromeier, D. Solecki, and E. Wimmer, submitted for publication.
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↵2 M. Gromeier and E. Wimmer, unpublished results.
- Abbreviations:
- CNS
-
central nervous system
- bp
-
base pair(s)
- AP
-
activator protein
- NRF
-
nuclear respiratory factor
- EMSA
-
electrophoretic mobility shift assay
- PCR
-
polymerase chain reaction
- DTT
-
dithiothreitol
- RT
-
reverse transcriptase
- E
-
embryonic day
- FPIV
-
foot print IV
- TBE
-
Tris/borate/EDTA
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- Received September 28, 1999.
- Revision received December 29, 1999.
- The American Society for Biochemistry and Molecular Biology, Inc.
