Comparison of the A2 Gene Locus in Leishmania donovani and Leishmania major and Its Control over Cutaneous Infection*

In Old World Leishmania infections, Leishmania donovani is responsible for fatal visceral leishmaniasis, and L. major is responsible for non-fatal cutaneous leishmaniasis in humans. The genetic differences between these species which govern the pathology or site of infection are not known. We have therefore carried out detailed analysis of the A2 loci in L. major and L. donovani because A2 is expressed in L. donovani but not L. major , and A2 is required for survival in visceral organs by L. donovani . We demonstrate that although L. major contains A2 gene regulatory sequences, the multiple repeats that exist in L. donovani A2 protein coding regions are absent in L. major , and the remaining corresponding A2 sequences appear to represent non-ex-pressed pseudogenes. It was possible to restore amastig-ote-specific A2 expression to L. major, confirming that A2 regulatory sequences remain functional in L. major . Although L. major is a cutaneous parasite in rodents and humans, restoring A2 expression to L. All plasmid constructs used in this study have been described previously (7, 10). The procedure for transfection was as described (13). The transfected L. major promastigotes were selected and maintained in medium containing 50 (cid:1) g/ml of G418. The pooled transfectants were used for all subsequent studies (Northern blot, Western blot, and mice infection experiments). Southern, Northern, and Western Blot Analysis— Southern, Northern, and Western blot analysis were performed as described previously (7). The DNA probes were as follows: A2, and EcoR I fragment from L. donovani A2 (cid:2) untranslated region (UTR) 1 A2rel, HI and EcoR fragment the A2rel-specific probe, 946-bp fragment donovani A2rel gene and A2rel-specific 1274-bp EcoR V fragment donovani (cid:2) A2rel A2 protein analysis, anti-A2 monoclonal antibody C9 used as described previously Infection in Mice— For cutaneous female BALB/c mice (Charles River Breeding Laboratories) weighing 18–20 g were infected subcutaneously with 5 (cid:3) 10 6 stationary-phase promastigotes in their hind footpads. Disease progression was monitored by weekly caliper measurement of footpad swelling. Some infected footpads were removed and homogenized. The recovered amastigotes were cultured in promastigote culture medium, and the Leishmania parasite burdens were determined by limiting dilution. Some transformed promastigotes were then shifted to amastigote culture medium overnight and subjected to Western blot analysis.

Leishmania protozoa are the causative agents of human leishmaniasis, which is a serious infectious disease throughout the developing world (1). Leishmaniasis includes a wide range of pathologies ranging from severe lethal visceral infections to self-healing cutaneous lesions, and the specific pathologies are closely related to the species of Leishmania (2). For example, L. donovani causes visceral leishmaniasis, known as Kala Azar in India and the Sudan, which is fatal if not successfully treated and is characterized by irregular bouts of fever, substantial weight loss, swelling of the spleen and liver, and anemia (1,2). In comparison, L. major infections, common throughout the Middle East, result in cutaneous lesions that usually heal spontaneously (1,2). The molecular differences between the different species of Leishmania that govern the outcome of infection and pathogenesis in the human host are unknown.
Leishmania protozoa are transmitted to humans through the bite of an infected sandfly. In the sandfly vector, Leishmania exists as flagellated promastigotes, and once they enter the mammalian host after a blood meal, they differentiate into the non-flagellated amastigotes, where they multiply exclusively in the phagolysosomal compartment of macrophages (3,4). Differentiation from promastigotes to amastigotes is a critical step for the establishment of infection in macrophages, and therefore genes that are specifically expressed in the amastigote stage are likely to be essential for survival in the mammalian host.
The A2 gene family was first identified in L. donovani by virtue of its expression, which is specific to the amastigote stage of the life cycle (5), and it has been established that the A2 protein is among the few widely accepted amastigote-specific molecular markers identified to date (reviewed in Ref. 6). The A2 protein represents a virulence factor because L. donovani amastigotes deficient in A2 protein through the use of antisense RNA (7) or gene knock-outs (8) are attenuated with respect to survival in visceral organs of infected mice. Moreover, intravenous injection of L. major genetically engineered to express A2 produced higher infection levels in the spleen than control L. major, further supporting the argument that A2 enhances survival in resident macrophages of visceral organs (8).
Multiple copies of the A2 gene are clustered on the 850-kb chromosome of L. donovani, which encode a family of A2 proteins, each containing a secretory leader sequence followed by a 10-amino acid sequence that is repeated from 40 to Ͼ90 times, depending on the individual A2 gene (5,9,10). Consistent with the A2 gene family structure and expression, a family of A2 proteins ranging from 42 to 100 kDa is specifically expressed in amastigotes but not in promastigotes (7,10). Anti-A2 antibodies have been detected in Ͼ90% of the sera samples from visceral leishmaniasis patients, confirming that the A2 proteins are expressed in the human host (11). Preliminary karyotype analysis by Southern blotting revealed that L. dono-vani A2 protein coding sequences are not present in the genome of all Leishmania species and, notably, appeared to be absent in L. major and L. tropica (11).
Few Leishmania species-specific genes have been identified, and it was therefore possible in the present study to define the genetic basis for the difference in A2 gene structure between L. donovani and L. major. Interestingly, L. major does contain A2 genes, but they are not expressed and lack the multiple repeats in the protein coding regions and therefore represent nonexpressed pseudogenes. Because L. major causes cutaneous infections, it was therefore possible to examine the phenotype associated with restored A2 expression on cutaneous infection and the host cellular immune response to A2-expressing L. major. The results of this study suggest that the inability of L. major to express A2 has contributed to its tropism for cutaneous infections.

EXPERIMENTAL PROCEDURES
Mice-BALB/c mice and C57BL/6 (B/6) were purchased from Charles River Breeding Laboratories or from the Division of Cancer Treatment, National Cancer Institute (Frederick, MD). All mice were maintained in the animal care facility under pathogen-free conditions.
Leishmania Cells and Culture Conditions-For cutaneous infection in BALB/c mice and Northern blot analysis, L. major Friedlin V9 strain and L. donovani 1S/Cl2D strains were used. L. major and L. donovani promastigotes were routinely cultured at 27°C in M199 medium (pH 7.4) supplemented with 10% heat-inactivated fetal bovine serum. The L. donovani 1S/Cl2D strain can replicate continuously as axenic amastigotes in vitro (12). L. donovani differentiation into amastigotes was performed by shifting the promastigotes to amastigote culture conditions overnight (37°C in RPMI 1640, pH 5.5), which mimics the host macrophage phagolysosome compartment as described previously (12). Likewise, L. major promastigote cultures were resuspended in the amastigote culture conditions overnight, and this resulted in viable amastigote-like cells, which were unable to proliferate.
Plasmid Construction and Transfections-All plasmid constructs used in this study have been described previously (7,10). The procedure for transfection was as described (13). The transfected L. major promastigotes were selected and maintained in medium containing 50 g/ml of G418. The pooled transfectants were used for all subsequent studies (Northern blot, Western blot, and mice infection experiments).
Infection in Mice-For cutaneous infection, female BALB/c mice (Charles River Breeding Laboratories) weighing 18 -20 g were infected subcutaneously with 5 ϫ 10 6 stationary-phase promastigotes in their hind footpads. Disease progression was monitored by weekly caliper measurement of footpad swelling. Some infected footpads were removed and homogenized. The recovered amastigotes were cultured in promastigote culture medium, and the Leishmania parasite burdens were determined by limiting dilution. Some transformed promastigotes were then shifted to amastigote culture medium overnight and subjected to Western blot analysis.
Parasite Quantitation-Parasite loads in the ears were determined as described previously (16). Briefly, the ventral and dorsal sheets of the infected ears were separated, deposited dermal side down in Dulbecco's modified Eagle's medium containing 100 units/ml of penicillin, 100 g/ml of streptomycin, liberase CI enzyme blend (Roche Molecular Biochemicals, 50 g/ml), and incubated for 1 h at 37°C. The sheets were cut into small pieces and homogenized using a Teflon-coated microtissue grinder in a microcentrifuge tube containing 100 l of M199/S. The tissue homogenates were filtered using a 70-m cell strainer (Falcon Products, Inc., St. Louis, MO) and serially diluted in a 96-well flat bottom microtiter plate containing biphasic medium, prepared using 50 l of NNN medium containing 30% of defibrinated rabbit blood and overlaid with 50 l of M199/S. The number of viable parasites in each ear was determined from the highest dilution at which promastigotes could be grown out after 7 days of incubation at 26°C. The number of parasites was also determined in the local draining lymph nodes (retromaxilar). The lymph nodes were recovered and mechanically dissociated using a pellet pestle and then serially diluted as above.
For cytokine measurement in culture supernatants, pooled cells from draining lymph nodes were resuspended in RPMI 1640 containing fetal bovine serum/penicillin/streptomycin at 6 ϫ 10 6 cells/ml, and 0.1 ml was plated in U-bottomed, 96-well plates. Cells were incubated at 37°C, 5% CO 2 with uninfected or L. major-infected bone marrow-derived dendritic cells. Interferon (IFN)-␥ and interleukin (IL)-10 production in 48-h culture supernatants were quantitated by enzyme-linked immunosorbent assay according to the manufacturer's instructions (Endogen).
Macrophage and Dendritic Cell Infections-For the migration experiments, bone marrow-derived dendritic cells and macrophages were obtained. Briefly, bone marrow cells were cultured in the presence of 20 ng/ml of granulocyte/macrophage-colony stimulating factor (to generate dendritic cells) or RPMI 1640 supplemented with 30% L929 conditioned medium (for macrophages). After 6 days, cells were collected, spun at 1500 rpm for 10 min, and seeded onto 6-well non-tissue culture-treated plates at a concentration of 3 ϫ 10 6 cells/well. Metacyclic promastigotes from the different strains were selected, and cells were infected at a ratio of 10 parasites:1 cell. Twenty-four h later, cells were detached from the wells using a syringe pestle, washed, and dyed with chloromethylbenzoylaminotetramethyl rhodamine, Molecular Probes, Eugene, OR). Briefly, cells were resuspended in PBS and dyed for 15 min at room temperature with 5 g/ml of the red fluorescent dye, washed three times with PBS/2% bovine serum albumin, cytospun, and stained with Diff Quick (Dade Behring, Dudinger, Switzerland) to determine the percentage of infection. In every case the percentage of infected cells was Ͼ80%, and it was comparable among the groups. Cells were resuspended at a final concentration of 10 7 infected macrophages/dendritic cells/ml.
Adoptive Transfer of Infected Cells-C57Bl/6 mice were anesthetized with 50 mg/kg of ketamine (Wyeth Fort Dodge, Iowa) solution. Infected or uninfected cells were inoculated in the ear dermis in a volume of 10 l, and 24 h later ears and draining lymph nodes from injected mice were collected. Ear dorsal and ventral sheets were separated with forceps and deposited dermal side down in RPMI 1640 supplemented with 10% fetal calf serum and antibiotics. Cells were allowed to migrate out of the ear for 16 h. The remaining ear tissue was incubated with Liberase Cl, cut in small pieces, and filtered through a 70-m nylon cell strainer (Becton-Dickinson Mountain View, CA) before being washed twice in RPMI 1640, NaHCO 3 , penicillin/streptomycin/gentamycin, 10% fetal calf serum, and 0.05% DNase (Sigma). Cells from draining lymph nodes were mechanically dissociated to single-cell suspensions, and the three cell compartments (cells migrating out of the ear dermis, ear tissue, and lymph node cells) were fixed in 4% paraformaldehyde. For the analysis of the presence of labeled cells, data were collected and analyzed using CELLQuest software and a FACScalibur flow cytometer (Becton Dickinson) for the presence of positive cells on the FL-2 channel. The number of cells that spontaneously migrated out of the ear was determined as the number of labeled cells tracked in the media divided by the total number of cells tracked in every compartment (ears, medium, and draining nodes).

RESULTS
Comparison of the A2 Gene Structure in L. donovani and L. major-Few genetic differences have been described between L. donovani and L. major, and therefore a comparison of the A2 gene loci provided an opportunity to perform a detailed comparison of a virulent gene family that differs between these Old World Leishmania species. Because A2rel genes neighbor the A2 genes and are conserved between L. donovani and L. major (11), an L. donovani A2rel DNA probe was used to identify A2rel DNA-containing L. major cosmids. Three nonoverlapping cosmids termed L5114, L4134, and L610 derived from chromosome 22 were isolated, and their orientation is shown in Fig. 1A. Cosmid L4134 was chosen for DNA sequencing. As also shown in Fig. 1A for comparison, the A2 gene family (white boxes) in L. donovani are clustered in tandem array alternating with the neighboring A2rel gene family (black boxes), and there is more than one cluster per chromo-some as described previously for L. donovani (8). However, in comparison with L. major, the sequence data revealed that the overall organization of the A2 (white boxes) and A2rel (black boxes) genes differed between L. donovani and L. major. It was, however, of interest that L. major did contain A2 gene sequences and that the flanking regions (shown as A2rel 5Ј and A2rel 3Ј) of the A2-A2rel gene clusters were very well conserved between L. donovani and L. major (86% identity). Moreover, as with L. donovani, there is more than one cluster of A2 genecontaining sequence per chromosome in L. major. Nevertheless, there are many more copies of the A2 gene sequences (white boxes) in L. donovani than in L. major. The most remarkable difference was that nearly the entire A2 protein coding sequences in the open reading frame (ORF) were absent in the A2 genes in L. major. As a result, the 10-amino acid encoding repeat units of the ORF that are present in all of the L. donovani A2 genes were absent in L. major. Only one 10amino acid unit remains in the L. major A2 genes preceded by the leader sequence (Fig. 1, B and C). As a result, the predicted corresponding L. major A2 protein would only be 53 amino acids, of which the first 32 N-terminal amino acids are a functional leader sequence. Remarkably, although most of the A2 protein coding sequence was absent in the A2 genes in L. major, the 5Ј UTR and the 3Ј UTR of the RNA non-coding region were Ͼ90% identical between L. donovani and L. major. This is unusual because protein coding sequences generally remain more conserved than the non-coding sequences, but the opposite is evident when comparing L. major and L. donovani A2 genes.
Southern blot and PCR analysis of L. major genomic DNA and the other A2 cosmids derived from L. major confirmed that L. major has only the truncated version of the A2 genes shown in Fig. 1, B and C, and does not have the multiple copies of the alternating A2-A2rel genes as in L. donovani (data not shown). Taken together, these data revealed significant and unpredicted differences in the A2-A2rel gene clusters in L. major and L. donovani, where L. major has much fewer copies of the A2 genes, and more remarkably, the majority of the protein coding sequences within the remaining A2 genes are absent.
Comparison of A2 Gene Expression in L. donovani and L. major-Northern blot analysis was performed to compare the expression of the A2 gene family in L. donovani and L. major using probes specific to the conserved 3Ј UTR. As reported previously (5,8), the A2 genes were abundantly expressed in L. donovani amastigotes and are expressed at much lower levels in promastigotes. As demonstrated in Fig. 2, it was not possible to detect any A2 gene transcripts in L. major by this overexposed Northern blot analysis using a conserved 3Ј UTR probe, demonstrating these truncated L. major A2 genes are not transcribed or their transcripts are not stable. Taken together, the truncated A2 genes in L. major are not expressed, and this together with the data presented in Fig. 1 strongly suggests that the remaining truncated A2 genes in L major represent pseudogenes, one of the few such examples in Leishmania.
Conserved A2 3Ј UTR Function in L. major and the Effect of A2 on L. major Proliferation in Culture and in Macrophages-As detailed above, there are significant differences in the A2 gene structure and expression between L. donovani and L. major. However, it was also evident that the A2 3ЈUTR regulatory sequence was conserved in L. donovani and L. major. It has been demonstrated previously that the 3Ј UTR sequence was responsible for mediating amastigote-specific expression in L. donovani (9), and that it was possible to express A2 in L. major (8). It was therefore necessary to determine whether the A2 3Ј UTR sequence retained the ability to mediate amastigote-specific expression in L. major. The tandemly repeated A2/A2rel genes are not present in the L. major cosmids L5114, L4134, and L610, but a truncated version of the A2 genes was identified in these L. major cosmids. L. donovani and L. major also contain the same conserved 5Ј flanking sequences (5Ј A2rel, wavy/black box) and 3Ј flanking sequences (3Ј A2rel, black/striped box) genes, which flank the tandemly repeated A2/A2rel genes in L. donovani. B, comparison of the A2 genes in L. donovani and L. major. The L. donovani A2 protein encoding ORFs are of various lengths ranging from 1.2 to 2.6 kb, depending on the member of the A2 gene family. In L. major, the A2 ORF is only 160 bp. Both L. donovani and L. major do, however, contain the same conserved A2 transcribed 5Ј UTR and 3Ј amastigote-specific regulatory UTR sequences. C, comparison of the L. donovani and L. major A2 protein coding ORF sequences. In L. donovani, the A2 gene ORFs encode a leader sequence (black boxes) followed by multiple 10 amino acids encoding repeat units (white boxes), followed by a short 7-amino acid, C-terminal sequence (gray box), yielding proteins that range from 42 to 100 kDa depending on the number of repeats, as detailed previously (5,10). In comparison, the L. major A2 gene ORF encodes the same leader sequence (black boxes) and the C-terminal sequence (gray box) as in L. donovani but only one unit of the 10-amino acid repeat (white box).
L. major promastigotes were transfected with the A2-expressing plasmid pKsneoA2, which contained a complete copy of the L. donovani A2 gene including the 5Ј UTR, A2 protein coding region, and the regulatory 3Ј UTR as shown in Fig. 3A. Promastigotes were also transfected with a control pKsneo plasmid with no A2 coding sequences. Western blot analysis was carried out to determine whether A2 expression could be induced under amastigote culture conditions in the transfected L. major culture. As shown in Fig. 3B, it was possible to induce A2 expression by shifting the culture to amastigote-like culture conditions overnight (pH 5.5, 37°C). As described above, the sequence of L. major A2 3Ј UTR has Ͼ90% identity to A2 3Ј UTR in L. donovani, suggesting that the L. major A2 3Ј UTR and associated regulatory factors remained functional in L. major with respect to mediating amastigote-specific expression.
Although it has been shown that ectopic expression of A2 enhanced the ability of L. major to survive in the spleen of BALB/c mice after intravenous infection (8), it was necessary to study other relevant phenotypes of A2-expressing L. major. It was determined whether A2 expression altered the growth properties of L. major in axenic culture or in in vitro cultured macrophages. As shown in Fig. 3C, L. major transfected with the pKsneoA2 plasmids grew equally well as the control transfected L. major under axenic culture conditions. Likewise, A2expressing and the control L. major infected and replicated equally in bone marrow-derived macrophages after infection with metacyclic parasite cultures (Fig. 3, D and E). Taken together, these data demonstrate that it was possible to reconstitute amastigote-specific gene expression of A2 in L. major, and this did not alter the ability to replicate in axenic culture or replicate in in vitro cultured macrophages.
Subcutaneous Infection of A2-expressing L. major in Genetically Susceptible BALB/c Mice-L. major infections cause cutaneous pathology, and the preceding gene structure and experimental data revealed that L. major lacks the ability to express the A2 gene. This therefore provided an excellent opportunity to determine whether ectopic A2 expression would influence the development of cutaneous leishmaniasis by L. major in relevant murine infection models. The A2-transfected and control L. major promastigotes described above were initially introduced subcutaneously into the footpad of BALB/c mice, and the infection was monitored by measuring the foot-pad thickness for several weeks. Up to the first 8 weeks, the A2-expressing L. major did induce swelling of the footpad at levels equal to the control infected mice (Fig. 4A). However, after this initial period, the footpad swelling in the A2 group was significantly reduced in size. Moreover, footpad ulceration typically developed in the mice infected with the control L. major on average at 12 weeks after infection, but no ulceration of lesions developed in mice infected with the A2-expressing L. major. Isolation of parasites from the infected footpads at 10 weeks after infection revealed that the mice infected with control L. major contained six to eight times more parasites than did footpads infected with the A2-expressing L. major. These observations argued that expression of A2 protein impaired the ability of L. major amastigotes to establish cutaneous infections.
The preceding experiments were carried with L. major expressing a single copy of the A2 gene. However, there are at least 11 copies of A2 genes in L. donovani-encoding proteins ranging from 45 to 100 kDa, depending on the number of repeat sequences within each gene (8,10). It was therefore necessary

FIG. 3. Conserved A2 3 UTR function in L. major with respect to mediating amastigote-specific expression.
A, schematic representations of the relevant regions of the A2 expression construct (pKs-neoA2) and the control plasmid (pKsneo). Construct pKsneoA2 contains the A2 protein coding sequence and the A2 5Ј and 3Ј UTR sequences required for proper transcript processing and amastigote-specific expression in L. donovani. B, Western blot analysis of A2 protein in wild-type (WT) and transfected L. major cells using an A2-specific monoclonal antibody. A, amastigote-like cells; P, promastigotes. Note: the A2 protein was detectable in the pKsneoA2-transfected cells only after they were shifted to amastigote culture conditions, confirming that the A2 3Ј UTR amastigote-specific regulatory sequence is functional in L. major. C, growth curves of pKsneo-and pKsneoA2-transfected L. major promastigotes in vitro. Cell growth was monitored daily by measuring A 600 . D, percentage of bone marrow-derived macrophages from BALB/c mice infected with pKsneo-and pKsneoA2-transfected L. major over time. E, proliferation of pKsneo-and pKsneoA2-transfected L. major metacyclic promastigotes in bone marrow-derived macrophages. The data are the means Ϯ S.D. of three replicates; the experiments have been repeated two times with similar results. to confirm the above observation using cosmid-transfected L. major capable of expressing three copies of the A2 genes. As shown in Fig. 4B, Western blot analysis of L. major transfected with the A2 locus containing cosmid revealed the presence of three A2 proteins of 44, 50, and 90 kDa, which were expressed specifically under amastigote-like culture conditions. This further confirmed the preceding data arguing that the A2 3Ј UTR is functional in L. major with respect to mediating amastigotespecific expression. Although the A2 cosmid-containing and control L. major were both less virulent in bone marrow-derived macrophages than the above-described plasmid-transfected cultures, they did however have similar in vitro infection kinetics in macrophages and proliferated equally well in culture (data not shown). We therefore proceeded to infect BALB/c mice subcutaneously with control and A2 cosmid-transfected L. major, and the footpad thickness was followed as described above. As shown in Fig. 4C, the A2 cosmid-transfected L. major failed to induce any footpad swelling and resulted in no detectable footpad infection compared with the control cosmid containing L. major, which was fully virulent with respect to causing cutaneous leishmaniasis. The relative inhibitory effect on cutaneous infection was significantly more dramatic with the A2 cosmid-transfected L. major than with the A2 plasmidtransfected L. major, suggesting that cosmid-derived multiple copies of A2 protein impaired the development of cutaneous infection more effectively than expression of a plasmid-derived single copy of the A2 protein. It is noteworthy that we cannot rule out the possibility that other genes on this cosmid may also contribute to the phenotype. We have, however, determined that the A2rel genes are tandemly repeated with the A2 genes as outlined in Fig. 1, and are also present on this cosmid, could not enhance the A2 phenotype when cotransfecting A2 and A2rel-expressing plasmids into L. major (data not shown).
Cutaneous Infection of A2-expressing L. major in Genetically Resistant C57BL/6 Mice-The above experiments were carried out on the footpads of genetically susceptible BALB/c mice and suggested that restoring A2 expression to L. major resulted in suppression of cutaneous pathology. It was, however, necessary to further investigate this phenotype in an infection model that more closely mimics the pathogenesis of human cutaneous leishmaniasis (16). We therefore examined the effect of A2 expression on the cutaneous pathogenesis by L. major using low-dose infection with 1000 purified metacyclic L. major promastigotes after intradermal inoculation in both ears in genetically resistant C57BL/6 mice. Infection was subsequently monitored by measuring the diameter of the ear lesion over the course of the infection. As shown in Fig. 5A, ears of C57BL/6 mice infected with the control cosmid-transfected L. major developed lesions as early as 2 weeks after infection, peaked at 7 weeks after infection, and healed spontaneously 17-20 weeks after infection, this being the typical L. major infection course in resistant C57BL/6 mice (16). However, consistent with what was observed in the footpad infections in susceptible BALB/c mice, the A2 cosmid-transfected L. major failed to induce any lesion in C57BL/6 mice ears. Moreover, parasite loads in ears and local draining lymph nodes were much lower in A2-expressing L. major infected mice than in control L. major-infected mice (Fig. 5, B and C). The amount of parasites (1258 parasites Ϯ 630.9 parasites/ear, Fig. 5B) isolated from mice infected with A2-expressing L. major 6 weeks after infection was similar to that inoculated during original challenge infection, indicating the lack of growth of the A2-expressing L. major in ears of infected C57BL/6 mice. These data confirmed that A2 expression in L. major suppresses cutaneous leishmaniasis.
It was necessary to examine the immune response toward the A2-expressing L. major to determine whether the suppression of survival in the skin was attributable to an enhanced acquired immune response against A2-expressing L. major or attributable to an innate effect of A2 on L. major biology in the skin epithelium. Cells from draining lymph nodes of C57BL/6

FIG. 4. A2 expression in L. major impairs the development of cutaneous infection in the footpad of susceptible BALB/c mice.
A, footpad swelling measurements in mice infected with A2-expressing L. major and control L. major. 5 ϫ 10 6 stationary-phase promastigotes were subcutaneously inoculated into the hind footpads (four mice/ group), and the thickness of footpads was measured weekly for 17 weeks. Data are the mean Ϯ S.D. for the four mice in each group. This experiment was repeated four times with similar results. B, detection of A2 protein with anti-A2 monoclonal antibody C9 in the A2 cosmidtransfected and control cosmid-transfected (CosTL) L. major cells cultured overnight at 37°C, pH 5.5. The sizes of the expressed A2 proteins are as indicated. C, footpad thickness of mice infected with CosA2-or CosTL-transfected L. major promastigotes. Data are the mean Ϯ S.D. for four mice/group. This experiment was repeated three times with similar results. mice infected for 6 weeks with control or A2 L. major were pooled and incubated with uninfected or L. major-infected bone marrow-derived dendritic cells. The culture supernatants were collected after 48 h and measured for IFN-␥ and IL-10 production. As shown in Fig. 6, the control L. major infection in C57BL/6 mice induced high expression of IFN-␥ and moderate expression of IL-10, indicating a Th1-dominated immune response. However, neither IFN-␥ nor IL-10 cytokines were detected in the draining lymph nodes of mice infected with A2-expressing L. major, suggesting that the host immune response was not actively involved in suppressing the infection with A2-expressing L. major. These data argue that the suppression of cutaneous infection was attributable to an innate effect of A2 on L. major survival in the skin.
Macrophages Infected with A2-expressing L. major Migrated Out of the Ear Dermis Better Than Control L. major-infected Macrophages-The inability of A2-expressing L. major to thrive in cutaneous or subcutaneous inoculation sites of mice is characteristic of L. donovani. It was therefore of interest to determine whether A2 expression in L. major induced other phenotypes characteristic of L. donovani. We have recently developed a system to compare the ability of macrophages infected with different species of Leishmania to migrate out of the dermal matrix. Murine macrophages labeled with a fluorescent dye and infected with different strains of L. major or L. donovani are injected in the ears of C57BL/6 mice, and 24 h later, ears are recovered, and the cells sedimenting from the dermis during 16 h are collected and analyzed by fluorescenceactivated cell sorter. After these analyses, we found that macrophages infected with visceralizing species migrated out of the dermis in higher levels than macrophages infected with cutaneous species (data not shown). It was therefore possible to determine whether macrophage infected with A2-expressing L. major would behave like L. donovani-infected macrophages The murine macrophages or dendritic cells were infected with metacyclic promastigotes of wild-type L. major, wild-type L. donovani, and L. major containing A2 cosmid or control cosmid, labeled with a fluorescent dye and injected in the ears of C57BL/6 mice. Twenty-four h later, the ears and draining lymph nodes were recovered, and the cells sedimenting from the dermis during 16 h were collected and analyzed by fluorescence-activated cell sorter. The percentage of cells that migrated out of the ear was determined as the number of labeled cells tracked in the media divided by the total number of cells tracked in the ears, medium, and draining nodes. The data shown are the mean Ϯ S.D. of three separate experiments.
including L. donovani, L. chagasi, and L. infantum but not in cutaneous leishmaniasis species such as L. major, L. tropica, and L. braziliensis (11), and this fits with the overall hypothesis that A2 plays a role in visceral leishmaniasis. However, the A2 coding sequences were also detected by karyotype analyses in L. mexicana complex including L. amazonenesis and L. mexicana (11), and the A2 gene products have been detected by Western blot analysis as three larger proteins of Ͼ200 kDa in L. mexicana. 2 Species from the New World L. mexicana complex are associated with diffuse cutaneous infections, and they are phylogenetically farther from the Old World species L. donovani and L. major (17). There may therefore be significant differences in the rest of the genome that allow L. mexicana to survive in the skin despite the presence of the A2 protein. Divergence in gene function between Old World and New World Leishmania species would not be without precedent because although lipophosphoglycan is clearly a virulence factor for L. major (20), it appears not to be a virulence factor for L. mexicana (21). Indeed, such phenotypic differences may also be related to the diversity in the host response to different species of Leishmania as discussed previously (22). The potential different roles of A2 proteins may also be associated with the different sizes and abundance of these proteins because A2 proteins are much less abundant in L. mexicana than in L. donovani. 2 It is also noteworthy that there has been reports of visceral Leishmania infections caused by L. tropica, a cutaneous Leishmania species (23,24). Although the host physical and immune status can also play important roles in determining the outcome of Leishmania infection (24), it is not known what caused the pathogenic change of these L. tropica parasites. These observations support the argument that A2 is not the only factor required for visceral infection; however, in the case of L. donovani, it does appear to be necessary for visceral infection (7,8). As the Leishmania genome project progresses (25,26), it will be of great interest to identify additional genomic differences between Leishmania species that may provide a better understanding of the tropism and pathogenesis of these pathogens.