PARP1-dependent Kinetics of Recruitment of MRE11 and NBS1 Proteins to Multiple DNA Damage Sites

FRAP analyses of PARP1-GFP in undamaged and microirradiated nuclei. a, the upper panel shows the time-lapse image collection of PARP1-GFP recovery after photobleaching in undamaged HeLa cells. The bottom panel shows FRAP images of PARP1-GFP inside the microirradiated regions. The undamaged nucleoplasm and microirradiated region where FRAP was performed are indicated by a white box. Bar, 5 μm. b, the plot shows normalized mean values of fluorescence intensity versus time in seconds after photobleaching.