Dose-dependent recruitment of PARP1 to locally induced DNA damage. a, linear region across the entire nucleus were microirradiated with varying laser power. The energy output of the laser is indicated for each individual cell. Bar, 5 μm. The insets show three-dimensional images of the redistribution of PARP1-GFP in the microirradiated region. b, human cells were microirradiated with increasing laser power. The cells were fixed readily after induction of DSBs by laser microirradiation and processed for immunodetection of γ-H2AX and PAR synthesis. c, the graph summarizes quantification of fluorescence intensities associated to γ-H2AX and PAR as a function of laser power. Data are presented as mean ± S.E. from at least 20 cells for each treatment.
