Site-specific Androgen Receptor Serine Phosphorylation Linked to Epidermal Growth Factor-dependent Growth of Castration-recurrent Prostate Cancer*

The androgen receptor (AR) is required for prostate cancer development and contributes to tumor progression after remission in response to androgen deprivation therapy. Epidermal growth factor (EGF) increases AR transcriptional activity at low levels of androgen in the CWR-R1 prostate cancer cell line derived from the castration-recurrent CWR22 prostate cancer xenograft. Here we report that knockdown of AR decreases EGF stimulation of prostate cancer cell growth and demonstrate a mechanistic link between EGF and AR signaling. The EGF-induced increase in AR transcriptional activity is dependent on phosphorylation at mitogen-activated protein kinase consensus site Ser-515 in the AR NH2-terminal region and at protein kinase C consensus site Ser-578 in the AR DNA binding domain. Phosphorylation at these sites alters the nuclear-cytoplasmic shuttling of AR and AR interaction with the Ku-70/80 regulatory subunits of DNA-dependent protein kinase. Abolishing AR Ser-578 phosphorylation by introducing an S578A mutation eliminates the AR transcriptional response to EGF and increases both AR binding of Ku-70/80 and nuclear retention of AR in association with hyperphosphorylation of AR Ser-515. The results support a model in which AR transcriptional activity increases castration-recurrent prostate cancer cell growth in response to EGF by site-specific serine phosphorylation that regulates nuclear-cytoplasmic shuttling through interactions with the Ku-70/80 regulatory complex.

The androgen receptor (AR) 3 is required for normal prostate development and the onset and progression of prostate cancer.
AR has a modular structure characteristic of steroid hormone receptors, with an NH 2 -terminal transcriptional activation domain, DNA binding domain, hinge region, and carboxyl-terminal ligand binding domain (1,2). AR mediates the biological effects of androgens by binding testosterone and dihydrotestosterone (DHT) with high affinity (3). Androgen binding in the ligand binding domain stabilizes AR through the NH 2 -and carboxyl-terminal N/C interaction that increases AR transcriptional activity (4). Androgen deprivation by surgical or chemical castration to treat advanced prostate cancer reduces AR transcriptional activity and promotes tumor regression.
Several mechanisms have been proposed to explain the emergence of castration-recurrent prostate cancer during androgen deprivation therapy (for review, see Ref. 5). AR transcriptional activity and CWR-R1 human prostate cancer cell proliferation are hypersensitive to DHT (6). AR localizes in the nuclei of prostate cancer cells despite low levels of circulating androgen and appears to mediate recurrent growth after androgen deprivation (7). This could be explained by the presence of sufficient testosterone or DHT to activate AR in the microenvironment of castration-recurrent prostate cancer tissue (8,9). On the other hand, cell culture studies suggest that AR transcriptional activity involves growth factor signaling under conditions of androgen deprivation.
HER2/neu, keratinocyte growth factor, insulin-like growth factor-1, and interleukin-6 have been reported to activate AR in the absence of androgen (10 -12). Epidermal growth factor (EGF)-dependent phosphorylation of transcriptional intermediary factor 2 and heregulin signaling through the HER2 and HER3 tyrosine kinases increase AR transactivation and alter the growth of CWR-R1 prostate cancer cells in response to low levels of androgen (13). HER2 and HER3 activation, possibly through autocrine signaling, contributes to cell proliferation during prostate cancer recurrence. Growth factor signaling contributes to the onset of castration-recurrent prostate cancer through cross-talk between AR and autocrine loops that drive prostate cancer growth in the androgen-deprived patient.
Despite evidence for AR transcriptional activity in castration-recurrent prostate cancer, there is little consensus regarding the mechanisms involved in growth factor-mediated AR phosphorylation (14). The present study determined the effects of EGF on AR phosphorylation, nuclear localization, gene transactivation, and prostate cancer cell proliferation. We demonstrate that AR is required for CWR-R1 prostate cancer cell growth in response to androgen or EGF and that DHT and EGF act synergistically to increase cell growth. EGF-dependent phosphorylation at MAP kinase consensus site Ser-515 in the AR NH 2 -terminal domain and protein kinase C consensus site Ser-578 in the AR DNA binding domain regulate AR nuclearcytoplasmic shuttling through interactions with the Ku-70/80 regulatory subunits of DNA-dependent protein kinase (DNA-PK). The studies suggest that AR phosphorylation in the P-box of the DNA-binding-domain first zinc module regulates AR transactivation in response to EGF signaling.

EXPERIMENTAL PROCEDURES
RNA Interference and Transcription Assays-Duplex AR-siRNA-3 was ucaaggaacucgaucguauuu (sense) and auacgaucgaguuccuugauu (antisense) sequences, and AR-siRNA-4 was gaaaugauugcacuauugauu (sense) and ucaauagugcaaucauuucuu (antisense) sequences (NCBI M20132) (SMART selection designed siRNAs, Dharmacon Inc., Lafayette, CO). The control was siCONTROL nontargeting siRNA pool (Dharmacon Inc.). CWR-R1 prostate cancer cells derived from the CWR22 castration-recurrent prostate cancer xenograft (6,15) were transfected using Effectene (Qiagen, Valencia, CA) with 10 nM AR siRNA or control siRNA duplex and 0.1 g of mouse mammary tumor virus luciferase reporter (MMTV-Luc). CWR-R1 cells were plated (1.6 ϫ 10 5 cells/well) in 12-well plates using Richter's improved minimal essential prostate growth medium (Irvine Scientific, Santa Ana, CA) or Dulbecco's modified Eagle's medium (DMEM, Invitrogen), each supplemented with 10 nM nicotinamide, 5 g/ml insulin, 5 g/ml transferrin, 5 ng/ml selenium, 100 units/ml penicillin, 100 g/ml streptomycin, and 2% fetal bovine serum (FBS). DMEM growth medium was further supplemented with 15 mM HEPES, pH 7.2. DNA and siRNA (220 l) containing per well 45 l of EC buffer (Qiagen), 1 l of enhancer, 1 l of Effectene reagent (Qiagen), and 200 l of 2% serum-containing medium was added to cell cultures containing 0.8 ml of fresh medium. The next day the medium was replaced with phenol red-free, serum-free Improved Minimal Essential Zinc Option medium (Invitrogen) with and without DHT, and EGF and incubations were continued for 24 h. For protein kinase C (PKC) inhibition studies cells were treated with and without calphostin (Calbiochem), a PKC inhibitor, 1 h before transfection. Cells were treated again the next day in serum-free medium with and without calphostin in the absence and presence of 10 ng/ml EGF. Human endometrial Ishikawa cells were transfected using FuGENE-6 (Roche Applied Science) as previously described (16) with 0.025 g of pCMV-AR and 0.1 l of PSA-Enh-Luc. Cells were harvested in 0.25 ml of lysis buffer containing 1% Triton X-100, 2 mM EDTA, and 25 mM Tris phosphate, pH 7.8 (17). Luciferase activity was measured using an automated LumiStar Galaxy multiwell plate reader luminometer (BMG Labtechnologies, Durham, NC). Transfection data are representative of at least three independent experiments.
Construction of Self-complementary Adeno-associated Virus (scAAV)-AR-siRNA and scAAV-control (CTR)-siRNA-pSilencer 1.0-U6 siRNA containing an RNA polymerase III promoter (18,19) (Ambion Inc., Austin, TX) and the Insert Design Tool for the pSilencer Vectors (Ambion) were used to generate hairpin siRNA encoding DNA oligonucleotide sequences based on the siRNA-3 sequence. A central loop sequence ttcaagaga and single overhang strand were added for cloning. Oligonucleotides that target AR were aaggaactcgatcgtatcattcaagagatgatacgatcgagttccttgatttttt (sense, 55 nucleotides) and aattaaaaaatcaaggaactcgatcgtatcatctcttgaatgatacgatcgagttccttggcc (antisense, 63 nucleotides) with 5Ј-EcoRI and 3Ј-ApaI terminal restriction sites. Control scAAV duplex oligonucleotides were ttctccgaacgtgtcacgtttcaagagaacgtgacacgttcggagaatttttt (sense, 53 nucleotides) and aattaaaaaattctccgaacgtgtcacgttctcttgaaacgtgacacgttcgagaaggcc (antisense, 61 nucleotides). Oligonucleotides were annealed by incubating at 90°C for 3 min in the presence of 46 l of annealing buffer containing 0.1 M potassium acetate, 2 mM sodium acetate, and 0.03 M HEPES-KOH, pH 7.4, followed by 1 h of incubation at 37°C. pSilencer 1.0-U6 was linearized with ApaI and EcoRI and ligated overnight at 25°C to the siRNA insert. Type II scAAV expression vectors ptrs-U1a-RFP-U6 and ptrs-U1a-green fluorescent protein and helper plasmids pXX6 (adenoviral helper genes) and pXX2 (AAV helper genes) were generously provided by Douglas M. McCarthy and Jude R. Samulski (Gene Therapy Center, University of North Carolina at Chapel Hill). p-trs-U1a-RFP-U6 contains a small nuclear RNA U1a promoter to drive expression of red fluorescent protein derived from pDSRED2-C1 (Clontech, Palo Alto, CA). The U6 promoter and duplex sequences were excised from pSilencer 1.0-U6 and cloned into ptrs-U1a-RFP-U6 linearized with NotI and KpnI. In-frame ligation was confirmed by sequencing. scAAV vectors produced in human embryonic kidney 293 cells using three-plasmid transfection were purified as described (20).
Human embryonic kidney 293 cells (2 ϫ 10 7 /dish) in DMEM containing 10% FBS were plated in twenty 15-cm dishes for 70 -80% confluency after 24 h. Each plate was passaged 1:4 in 15-cm dishes, and 16 h later the medium was replaced with complete Iscove's modified Dulbecco's medium (Invitrogen) containing 10% FBS and incubated for 3 h before transfection. pXX6 helper plasmid (90 g) was combined with 30 g of control or AR-siRNA-scAAV vector plasmid, 30 g of pXX2 helper plasmid, 0.25 M CaCl 2 , 0.25 M NaCl, 1.5 mM sodium phosphate, and 0.05 M Hepes, pH 7.2. Cells were incubated for 24 h, and medium was replaced with DMEM containing 2% FBS. Virus was collected 48 h post-transfection by lysing cells using 3 freeze-thaw cycles. scAAV vectors were purified by isopycnic centrifugation in CsCl ( ϭ 1.4 g/ml) using an SW41 rotor (Beckman Instruments) at 40,000 rpm for 48 h at 10°C. Fractions were collected and semiquantitated by slot-blot, and peak fractions were pooled and dialyzed in phosphate-buffered saline (PBS) overnight at 4°C.
To test transduction efficiency of the scAAV serotype II in CWR-R1 cells, cells were infected with scAAV type II ptrs-U1agreen fluorescent protein virus in medium containing 2% FBS. Cells were harvested 1 and 9 days post-infection. Green fluorescent protein expression in transduced cells was analyzed by fluorescence-activated cell sorting using a FACScan1 cytometer (BD Biosciences). Forward and side scatter parameters were set according to cell size, and the setting for fluorochrome detection was adjusted so that fluorescence intensity of uninfected negative control cells was within the first decade of the four-scale log plot.
Cell Proliferation Assays-CWR-R1 prostate cancer cells (1.6 ϫ 10 5 /well) in 12-well plates were allowed to grow in prostate growth medium for 24 h. Cells were infected with 10 3 viral particles/cell in medium containing 2% FBS by rocking for 30 min at room temperature and incubated overnight at 37°C in serumcontaining medium. Cells were transferred to serum-free, phenolred-free medium in the absence and presence of DHT and EGF. Duplicate wells were treated and assayed on days 1, 3, and 5 after infection. One-tenth volume of 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfo-phenyl)-2H-tetrazolium monosodium salt (WST-8, Dojindo Molecular Technologies, Gaithersburg, MD) was added to the wells and incubated for 2.5 h at 37°C, and absorbance was determined at 450 nm using a plate reader. The effect of inhibiting PKC on cell proliferation was assayed using cells plated in medium containing 2% FBS. The next day cells were treated with calphostin (Calbiochem), a PKC inhibitor, in the absence and presence of EGF. Proliferation assays were performed 1, 3, 5, and 7 days after treatment.
Immunoblotting was performed using monkey kidney COS-1 cells maintained in DMEM containing 2 mM L-glutamine, 10% bovine calf serum, penicillin, streptomycin, and 20 mM Hepes, pH 7.2. COS cells (2.5 ϫ 10 6 /10-cm dish) were transfected with 2 g of DNA using DEAE-dextran (25). Cells were treated 24 h after transfection with and without 10 ng/ml EGF for 5 h. For MAP kinase inhibition studies, cells were treated for 1 h before transfection with and without U0126 (Promega). The next day cells were serum-starved in the absence and presence of U0126, and media were replaced 24 h later with and without 10 ng/ml EGF for 5 h. Cells were rinsed with PBS, scraped into 1.5 ml of cold PBS, and centrifuged at 12,000 ϫ g for 2 min. The buffer was aspirated, and cells were resuspended and vortexed for 10 s in 50 -100-l buffer containing 1% Nonidet P-40, 0.15 M NaCl, 1% sodium deoxycholate, 0.1% SDS, 0.5 mM EDTA, 50 mM Tris-HCl, pH 7.5, 0.02 mg/ml pancreas extract, 1 mM phenylmethylsulfonyl fluoride, 0.005 mg/ml Pronase, 0.0005 mg/ml thermolysin, 0.003 mg/ml chymotrypsin, and 0.33 mg/ml papain (Roche Applied Science). After a 15-min incubation on ice, lysates were centrifuged for 15 min at 20,000 ϫ g, and protein concentrations were determined using the Bio-Rad assay. Protein (25 g) was incubated with and without 2.5 units -phosphatase (Sigma) in phosphatase buffer (Sigma) containing 2 mM MnCl 2 . To inhibit -phosphatase activity, lysates were incubated with 2.5 mM sodium vanadate, 10 mM sodium fluoride, and phosphatase Mixture Inhibitors 1/2 (Sigma) for 30 min at 25°C.
To express FLAG-tagged constructs in CWR-R1 cells, 3 g of DNA/5 ϫ 10 6 cells/10-cm dish was transfected using Effectene (Qiagen). The next day CWR-R1 cells were treated with and without 10 ng/ml EGF for 5 h, harvested in cold PBS from 5 pooled dishes for each treatment group, and lysed in immunoprecipitation buffer containing 0.5% Nonidet P-40, 10% glycerol, 0.05 M sodium fluoride, 0.15 M NaCl, 50 mM Tris-HCl, pH 7.6, and phosphatase and protease inhibitors. For immunoprecipitation of full-length AR expressed in COS cells, 0.5 M DHT was added to the lysis buffer. Approximately 1 mg of protein from two 10-cm COS cell dishes was precleared using Sepharose CL-4B (Sigma) and immunoprecipitated using anti-FLAG M2 affinity resin (Sigma). The final pellets were washed 3 times with immunoprecipitation buffer with and without 0.5 M DHT and resuspended in 60 l of 2ϫ sample buffer containing 3.3% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, and 20 mM Tris-HCl, pH 6.8, and analyzed by immunoblot.
Reactivity of AR phospho-Ser-578 antisera was determined by immunoblot of bovine serum albumin (BSA), BSA-coupled AR-(572-586) non-phosphorylated peptide and AR-(572-586) phospho-Ser-578 peptides 1 and 2 immunogens, and wild-type FLAG-AR-(507-660) and the S578A mutant. COS cells (2.5 ϫ 10 6 /10-cm dish) were transfected with 2 g of wild-type FLAG-AR-(507-660) and the S578A mutant, and 24 h later cells were placed in serum-free, phenol-red free media for 24 h and treated with and without 10 ng/ml EGF for 5 h. Cells were lysed in immunoprecipitation buffer containing phosphatase and protease inhibitors, immunoprecipitated using anti-FLAG M2 affinity resin (Sigma), and resolved on a 12% acrylamide gel containing SDS. Transfer blots were probed with AR phospho-Ser-578 antisera (1:100 dilution) and 2.5 g/ml AR52 antibody at room temperature for 2 h and with secondary antibody as described above. After chemiluminescence detection, membranes were rinsed with distilled water and incubated for 10 min at room temperature with 0.2% Ponceau S in 0.1% glacial acetic acid.
Immunocytochemistry was performed in COS cells (1 ϫ 10 5 cells/well) in 12-well plates with a coverglass (26). Cells were transfected using Effectene with 0.2 g of wild-type FLAG-AR-(507-660) or the S578A mutant and serum-starved 24 h. Cells were fixed for 10 min at room temperature with 4% paraformaldehyde in PBS, permeabilized for 5 min at 4°C with 0.2% Triton-X-100 in PBS, blocked for 1 h at room temperature with 0.5% bovine serum albumin in PBS (13), and incubated for 1 h in 0.5% bovine serum albumin containing AR52 antibody (2.5 g/ml) and for 30 min at room temperature with fluorescein isothiocyanate-conjugated anti-rabbit secondary antibody (1:75 dilution, Jackson ImmunoResearch Laboratories, Inc.). Slides were viewed using an Olympus BX60 microscope with original magnification of 40ϫ.
In Vitro Kinase Assays-GST-AR-(1-660) and GST-AR-(1-660)-S578A were expressed in BL21 Escherichia coli cells treated with 1 mM isopropyl-␤-thiogalactoside for 24 h at 16°C during log phase growth. Glutathione-agarose beads (Amersham Biosciences) were incubated for 1 h at 4°C with sonicated bacterial supernatants containing GST-AR fusion proteins. Beads were washed 3 times with PBS containing 1% Triton-X-100 followed by 3 washes with kinase buffer containing 10 mM EGTA, 0.1 M MgCl 2 , and 0.4 M Mes, pH 6.0. Part of the sample eluted with sample buffer was analyzed on 8 -12% acrylamide gradient gels containing SDS. A serial dilution of bovine serum albumin was analyzed in parallel to estimate protein recovery. Bound protein (10 -15 g) was assayed for PKC phosphorylation. GST beads were resuspended in 30 l of kinase buffer containing 10 or 100 Ci of [␥-32 P]adenosine triphosphate (3000 Ci/mmol) and 20 ng of purified PKC (catalytic subunit, rat brain, Calbiochem) in the absence and presence of 1 M unlabeled adenosine triphosphate and either 2.5 g of calf thymus histone H1 (Calbiochem) or 2.5 g of wild-type or mutant GST-AR-(1-660) fusion protein. After 10 min at 30°C, reaction products were resolved on 8 -12% acrylamide gradient gels containing SDS, which were dried and analyzed by autoradiography. To verify equal loading, gels were rehydrated in PBS and stained with Coomassie Blue, or half of the input resin was resuspended in 2ϫ SDS sample buffer and analyzed by immunoblotting using AR52 antibody. Autoradiographs were quantitated by densitometric scanning using Image-Pro Analyzer Software (Media Cybernetics, Inc., Bethesda, MD).
Nuclear Extraction-FLAG-AR fusion vectors were transfected into COS cells (2.5 ϫ 10 6 /10-cm dish) using DEAE dex-tran. The next day cells were serum-starved and 24 h later treated with 10 ng/ml EGF for 5 h. Nuclear extracts were prepared using the Nuclear Extract kit (Active Motif, Carlsbad, CA). Cells were washed with ice-cold PBS containing phosphatase inhibitors, scraped, and pelleted for 5 min at 2600 ϫ g, resuspended in 550 l of hypotonic buffer, and incubated for 15 min on ice. Detergent (25 l) was added, and samples were vortexed for 10 s at the highest setting. Cell suspensions were centrifuged for 30 s at 14,000 ϫ g, and cytoplasmic extracts were stored at Ϫ80°C. Nuclear cell pellets were resuspended in 50 l of complete lysis buffer containing 1 mM dithiothreitol and protease inhibitor mixture. Lysates were vortexed for 10 s, and suspensions were incubated for 30 min on ice on a rocking platform at 4°C. Extracts were vortexed 30 s and centrifuged 10 min at 14,000 ϫ g, and nuclear fractions were transferred to pre-chilled microcentrifuge tubes. Protein concentration was determined, and nuclear and cytoplasmic extracts were analyzed by immunoblotting using 2.5 g/ml AR52, tubulin-␣ (2 g/ml, Thermo Fisher Scientific, Fremont, CA) and Laminin B1 antibodies (2 g/ml, Active Motif).

AR-dependent CWR-R1 Prostate Cancer Cell Proliferation-
We investigated the requirement for AR in castration-recurrent prostate cancer cell growth using the CWR-R1 cell line that was derived from the castration-recurrent CWR22 xenograft of human prostate cancer (6). Growth studies were performed using CWR-R1 cells infected with control or ARtargeted siRNA-scAAV in the absence and presence of DHT and/or EGF. AR-siRNA-scAAV-infected cells grew more slowly than control cells in the absence and presence of 0.1 nM DHT (Fig. 1A). The Ͼ90% reduction in AR levels in CWR-R1 cells treated with and without DHT, assayed by immunoblot 2 and 5 days after AR-siRNA-scAAV infection (Fig. 1B), suggested that CWR-R1 cell growth was stimulated by AR both in the absence and presence of androgen.
EGF also stimulated control CWR-R1 cells to grow faster than cells infected with AR-siRNA-scAAV ( Fig. 2A, left panel). The reduction in AR levels determined by immunoblot of EGFtreated CWR-R1 cells 2 and 5 days after AR-siRNA-scAAV infection ( Fig. 2A, right panels) provided evidence that EGF stimulation of CWR-R1 cell growth was mediated in part by AR. However, the attenuated but significant growth response of AR-siRNA-scAAV-infected cells to EGF suggested that EGF also stimulated cell proliferation through signaling mechanisms that were independent of AR. The stimulatory effect of EGF together with DHT on growth of control and AR-siRNA-scAAV-infected cells was greater than DHT or EGF alone and approached maximal levels within 3 days (Fig. 2B, left panel). Growth of AR-siRNA-scAAV-infected cells treated with DHT and EGF was less attenuated compared with control cells even though AR levels were reduced as shown by immunoblot (Fig.  2B, right panels). The results indicate that AR increases CWR-R1 prostate cancer cell growth in response to DHT or EGF and that EGF and DHT act synergistically through AR.
Androgen-independent AR Transactivation-To pursue evidence that AR functions in castration-recurrent prostate cancer in the absence of androgen, we tested whether EGF can increase endogenous AR transcriptional activity in CWR-R1 cells using an MMTV-Luc reporter vector in the absence and presence of DHT. As expected, DHT increased AR transcriptional activity ϳ100-fold, with a further 3-fold increase after the addition of EGF (Fig. 3A). A similar effect of DHT with and without EGF on AR transcriptional activity was seen after transfection of a control siRNA oligonucleotide that did not reduce AR levels (Figs. 3, A and B, lane 5). However, AR-targeted siRNA oligonucleotide-3 that reduced AR levels (Fig. 3B, lane 3) greatly reduced AR transactivation, with only a 2-fold increase in activity remaining in the presence of DHT and an ϳ12-fold increase in response to DHT and EGF (Fig. 3A). In response to EGF alone, the ϳ3-fold increase in AR transcriptional activity in the presence of control siRNA was abrogated by AR duplex siRNA oligonucleotide-3 (Fig. 3C).
The results indicate that EGF can activate AR in the CWR-R1 prostate cancer cell line in the absence of androgen and that EGF and DHT act synergistically to increase AR transcriptional activity. These data together with the cell growth studies pre-FIGURE 2. Inhibition of EGF and EGF plus DHT-stimulated CWR-R1 cell growth using AR-siRNA-scAAV. A, CWR-R1 cells were infected with control or AR targeted siRNA-scAAV and cultured with or without 10 ng/ml EGF. Cell proliferation was assayed in the absence or presence of 10 ng/ml EGF as described in Fig. 1 (left panel). AR expression was determined by immunoblotting CWR-R1 cell lysates 2 and 5 days after infecting cells with control siRNA-scAAV or AR-siRNA-scAAV in the presence of EGF (right panels). B, CWR-R1 cells were infected with control or AR targeted siRNA-scAAV and treated with and without 10 ng/ml EGF and 0.1 nM DHT (left panel). Immunoblots of CWR-R1 cell AR were performed 4 days after infecting cells with control siRNA-scAAV or AR-siRNA-scAAV in the presence of 10 ng/ml EGF and 0.1 nM DHT (right panel). sented above support the hypothesis that AR activation by EGF is sufficient to drive prostate cancer cell growth.
EGF-dependent AR Phosphorylation-Sequence analysis using NetPhos 2.0 (27) indicated 15 consensus serine, threonine, or tyrosine phosphorylation sites between AR residues 507 and 660 that comprise part of the AR NH 2 -terminal region, the DNA binding domain, and hinge region (Fig. 4A). Immunoblots of wild-type FLAG-AR-(507-660) expressed in COS cells revealed a 21-kDa protein and, after treatment with EGF, an additional slower migrating 23-kDa form (Fig. 4B, lanes 1-3). The EGF-dependent slower migrating form was eliminated by treatment with -phosphatase in the absence but not in the presence of phosphatase inhibitors (Fig. 4B, lanes 3-8). The slower migrating 23-kDa band was also observed in response to EGF with FLAG-AR-(507-660)-C576A, which has a cysteine mutation in the first zinc module that eliminates DNA binding (data not shown). The appearance of an EGF-dependent and phosphatase-sensitive slower migrating form of FLAG-AR-(507-660) indicated that EGF induces phosphorylation at one or more sites between AR residues 507 and 660 independent of AR binding to DNA.
To identify the EGF-dependent AR phosphorylation site(s), single serine or threonine to alanine and tyrosine to phenylalanine mutations were introduced into FLAG-AR-(507-660) at the consensus phosphorylation sites highlighted in Fig. 4A. Immunoblots of cell extracts before and after treatment with EGF indicated that only the AR NH 2 -terminal MAP kinase consensus S515A mutation eliminated the slower migrating 23-kDa form of FLAG-AR-(507-660) when assayed in COS and CWR-R1 cells (Fig. 4C, lanes 1-4). This result provided evidence that AR is phosphorylated at Ser-515 in response to EGF. In addition, the PKC consensus site mutation S578A in the AR DNA binding domain increased the relative proportion of the slower migrating 23-kDa phospho-Ser-515 form in the presence of EGF (Fig. 4C, lanes 5 and 6). MAP kinase-dependent phosphorylation at AR Ser-515 was supported by the decrease in intensity of the slower migrating 23-kDa band after treatment with both EGF and increasing concentrations of the MAP kinase inhibitor, U0126 (Fig. 4D).
AR phosphorylation at Ser-578 was indicated by the reactivity of an AR phospho-Ser-578-specific antibody that recognized two AR-(572-586) phospho-Ser-578-conjugated peptides used as immunogens but not unphosphorylated AR-(572-586) (Fig. 5A,  lanes 3-5). The AR phospho-Ser-578-specific antibody also recognized the faster migrating 21-kDa form of wild-type AR-(507-919) but not the S578A mutant (Fig. 5B, upper panel, lanes [2][3][4][5], whereas both forms were detected using the AR52 antibody (Fig. 5B, lower panel). Treatment with -phosphatase reduced the reactivity of the phospho-Ser-578 antibody reactivity with wild-type AR-(507-919) (data not shown). The results suggest that the EGF-dependent increase in AR transcriptional activity and CWR-R1 cell growth are associated with MAP kinase-dependent phosphorylation at AR Ser-515 in the NH 2 -terminal region and modulation by phosphorylation at Ser-578 in the DNA binding domain.
AR Phosphorylation Alters Transcriptional Activity-We investigated further the link between EGF-dependent AR phosphorylation and increased AR transcriptional activity using full-length wild-type AR and serine to alanine mutants expressed with a PSA-Enh-Luc reporter in CWR-R1 cells. AR transcriptional activity increased ϳ3-fold in response to EGF in the absence and presence of DHT (Fig. 6A, upper panel). A similar response was seen with AR-S650A, which has a mutation in the previously reported Ser-650 phosphorylation site in the hinge region of AR (28,29). AR-S515A transcriptional activity increased in response to EGF and DHT, but overall  1-4) and presence of phosphatase inhibitors (lanes 5-8). C, COS cells (upper panel) and CWR-R1 cells (lower panel) were transfected with 2 g of wild-type (wt) FLAG-AR-(507-660) or the S515A and S578A mutants. Cells were serum-depleted for 24 h, treated with and without 10 ng/ml EGF for 5 h, collected, and lysed in the presence of phosphatase inhibitors for immunoprecipitation using FLAG affinity resin. AR52 antibody was used to detect wt and mutant FLAG-AR-(507-660) and an associated slower migrating band indicative of phosphorylation. Also indicated is the nonspecific IgG band. D, reduced AR Ser-515 phosphorylation by mitogen-activated protein kinase inhibitor, U0126. COS cells were treated in the absence (lanes 1-2) and presence of increasing concentrations of U0126 (lanes 3-8) for 1 h before transfection with 2 g of FLAG-AR-(507-660). The next day cells were serum-starved for 24 h in the absence and presence of U0126. Cells were treated again for 5 h with and without U0126 in the absence (lanes 1, 3, 5, and 7) and presence of 10 ng/ml EGF (lanes 2, 4, 6, and 8). FLAG-AR-(507-660) was detected using AR52 antibody, and ␤-actin served as the loading control. activity was less than wild type. In contrast, EGF did not increase transcriptional activity of AR-S578A in the absence or presence of DHT or when the S578A mutation was combined with the S515A or S650A mutation. When assayed by immunoblot (Fig. 6A, lower panel), expression of AR-S578A and AR-S515A was similar to wild-type AR, as was androgen-dependent AR stabilization that results from the AR N/C interaction (4,23).
To further establish a requirement for Ser-578 in the EGFdependent increase in AR transcriptional activity, we performed transcription assays in human endometrial cancer Ishikawa cells using the PSA-Enh-Luc reporter. In the presence of   4 and 5). The next day cells were transferred to serum-free medium and 24 h later treated for 5 h in the absence and presence of 10 ng/ml EGF as indicated. Cell extracts were immunoprecipitated using FLAG-M2 affinity resin and separated on a 12% acrylamide gel containing SDS, and transfer blots were incubated with AR anti-phospho-Ser-578 antisera (1:100 dilution, upper panel) and 2.5 g/ml AR52 antibody (lower panel) as described under "Experimental Procedures".
increasing concentrations of DHT, AR-S578A transcriptional activity was similar to wild-type AR (Fig. 6C). This differed from CWR-R1 cells where AR-S578A transcriptional activity was less than wild-type AR (Fig. 6A). However, in agreement with results using the CWR-R1 cell line, the EGF-dependent increase in wild-type AR transcriptional activity in the presence of DHT was diminished by the AR S578A mutation in Ishikawa cells (Fig. 6C). The results suggest that phosphorylation at AR Ser-578 is required for the AR transcriptional response to EGF.
Functional Effects of AR Phosphorylation by PKC-Ser-578 is a predicted consensus phosphorylation site for PKC, a kinase that acts downstream of EGF signaling (30). We performed in vitro kinase assays using the PKC catalytic subunit with wildtype GST-AR-(1-660) and the S578A mutant. PKC-dependent phosphorylation of GST-AR-(1-660) was reduced 30 -35% by the S578A mutation when equivalent amounts of protein were assayed by immunoblot (Fig. 7A,  lanes 1 and 2), where histone H1 served as a PKC substrate control (Fig. 7A, lane 3). When averaged over multiple experiments, the S578A mutation decreased GST-AR-(1-660) phosphorylation by ϳ50% (Fig. 7B). The EGF-dependent and independent increase in CWR-R1 cell growth was reduced by the PKC-specific inhibitor calphostin (Fig. 7C). The results sug-gestthatEGFsignalingthroughPKCdependent phosphorylation of AR Ser-578 increases AR transcriptional activity and AR-mediated CWR-R1 cell growth.
AR compartmentalization was also investigated by comparing nuclear and cytoplasmic extracts of cells expressing FLAG-AR-(507-660) and the S578A mutant before and after treatment with EGF. In agreement with the immunostaining results, wild-type FLAG-AR-(507-660) was distributed in both the nuclear and cytoplasmic fractions (Fig. 8B, lanes 2, 3, 7, and 8). The slower migrating 23-kDa phospho-Ser-515 form was more prominent in the nuclear fraction in response to EGF. FLAG-AR-(507-660)-S515A lacked the slower migrating 23-kDa form (Figs. 4 and 5) and distributed in both nuclear and cytoplasmic extracts similar to wild type (data not shown). However, in agreement with the immunostaining results, FLAG-AR-(507-660)-S578A was almost entirely nuclear, and the proportion of the phospho-Ser-515 form was increased (Fig.  8B, lanes 4, 5, 9, and 10). Parallel immunoblotting of nuclear laminin-B1 and cytoplasmic ␣-tubulin substantiated the subcellular fractionation results. Cell extracts contained similar amounts of wild-type and mutant FLAG-AR-(507-660) (data not shown), suggesting that the smaller amount of the S578A mutant in the cytoplasmic fraction did not result from degradation. The results suggest that phosphorylation at AR Ser-578 limits nuclear phosphorylation at Ser-515 and modulates AR nuclear-cytoplasmic shuttling.
Phosphorylation at AR Ser-578 Modulates AR Interaction with Ku-70/80-The Ku-70/80 regulatory subunits of DNA-PK were shown to interact with the progesterone receptor DNA binding domain (31), which shares sequence similarity with the AR DNA binding domain (32). Ku-70/80 subunits were also implicated in AR transcriptional recycling (33). To address the  5 g, lane 3). Assays were performed as described under "Experimental Procedures" using the PKC catalytic subunit in the presence of 10 Ci of [␥-32 P]adenosine triphosphate (upper panel). Parallel immunoblots were probed with AR52 antibody (lower panel). Samples were analyzed by autoradiography and band intensities measured by densitometry. NS designates a nonspecific phosphorylated band. B, data from four independent experiments described in A were averaged. C, inhibition of CWR-R1 cell proliferation by calphostin. CWR-R1 cells were plated and serum-starved the next day for 24 h and treated as described under "Experimental Procedures" in serum-free media with and without 10 ng/ml EGF alone or with 50 nM calphostin, a PKC inhibitor (day 0). Media and additives were replenished every other day over 7 days. Cell proliferation indexes were measured using WST-8 reagent on days 3, 5, and 7 after seeding. FIGURE 8. Increased nuclear localization of the AR S578A mutant. A, COS cells were transfected with 0.2 g of wild-type (wt) FLAG-AR-(507-660) or the S578A mutant and serum-starved the next day for 24 h. FLAG-AR-(507-660) and the S578A mutant are represented by green fluorescence detected using AR52 antibody and fluorescein isothiocyanate-conjugated secondary anti-rabbit antibody. Original magnification 40ϫ. B, nuclear and cytoplasmic fractions were prepared as described under "Experimental Procedures" and analyzed by immunoblotting using AR52 antibody that recognizes FLAG-AR-(507-660) wt and the S578A mutant. Laminin-B1 and ␣-tubulin served as nuclear and cytoplasmic extract controls, respectively.
influence of Ser-578 phosphorylation on AR interaction with Ku-70/80, we performed coimmunoprecipitation studies in COS cells using full-length wild-type FLAG-AR, the S578A mutant, and endogenous Ku-70/80. We found that Ku-70 and Ku-80 coimmunoprecipitated with FLAG-AR-S578A but only weakly with wild-type AR (Fig. 9A, upper two panels).
In similar experiments using FLAG-AR-(507-660), we found that the S578A mutant interacted with endogenous Ku-70 and Ku-80 to a greater extent than wild-type FLAG-AR-(507-660) in both COS (Fig. 9B) and CWR-R1 cells (Fig. 9C). Also, as seen with full-length FLAG-tagged AR, the interaction between wild-type FLAG-AR-(507-660) and Ku-70/80 increased in the response to EGF in both cell lines. A DNA binding mutant, FLAG-AR-(507-919)-C576A, interacted with Ku-70/80 to a similar extent as wild type (data not shown). The results suggest that EGF-dependent phosphorylation at AR Ser-578 modulates phosphorylation at Ser-515 and regulates AR interaction with Ku-70/80.

DISCUSSION
EGF Regulation of AR Transcriptional Activity-EGF signaling has been indirectly linked to increased AR transcriptional activity through the post-translational modification of AR coregulatory proteins. EGF increases phosphorylation of transcriptional intermediary factor 2, an SRC/p160 coactivator (17), and phosphorylation and multiple monoubiquitinylation of MAGE-11 of the melanoma antigen gene family (16). These changes stabilize the coregulator interaction with AR to increase AR transcriptional activity. In this report we provide evidence that EGF increases AR transcriptional activity through the coordinate phosphorylation of serine residues in the AR NH 2 -terminal and DNA binding domains.
We have shown that EGF increases CWR-R1 prostate cancer cell growth in an AR-dependent manner in the absence and presence of androgen. EGF acts synergistically with DHT to stimulate AR transcriptional activity and cell growth. EGF-dependent CWR-R1 prostate cancer cell proliferation was greatest in the presence of AR and DHT and was reduced by an inhibitor of PKC. One interpretation suggests that AR signaling in the absence and presence of DHT establishes a basal proliferation rate that is enhanced by EGF through mechanisms that include AR but are also independent of AR. EGF-dependent AR activation in the absence and presence of androgen is mediated by PKC-dependent phosphorylation at Ser-578 in the AR DNA binding domain and by MAP kinase-dependent phosphorylation at Ser-515 in the AR NH 2 -terminal region. The downstream functional effects of AR phosphorylation at these sites alter AR nuclear-cytoplasmic shuttling through interactions with Ku-70/80.
EGF-dependent AR Phosphorylation-Earlier mutagenesis studies demonstrated AR phosphorylation at Ser-81 and Ser-94 in the NH 2 -terminal region and at Ser-650 in the hinge region between the DNA and ligand binding domains (28). AR Ser-81 was suggested to be a substrate for the HER2-regulated kinase pathway (34), and AR phosphorylation at Ser-650 was linked to stress kinase modulation of AR transcriptional activity (29). However, mutations at these sites have relatively little effect on AR transcriptional activity (28,35). Mass spectrometry confirmed AR phosphorylation at Ser-81, -94, and -650 and identified Ser-16, -256, -308, and -424 as phosphorylation sites in the AR NH 2 -terminal region (14). AR NH 2 -terminal Ser-213 was phosphorylated in nonproliferating prostate epithelial cells (36), and Akt-mediated phosphorylation at AR Ser-213 and Ser-791 was linked to anti-apoptotic and proliferative effects in prostate cancer cells (37,38). However, the synergistic effects of Akt and AR on neoplastic proliferation of murine prostatic epithelium were independent of phosphorylation at these sites (39). More recently, prostate cancer growth was linked to Src and Ack1-mediated phosphorylation of AR tyrosine residues 267 and 363 in the AR NH 2 -terminal region (40).
Our current studies focused on the AR DNA binding domain and flanking NH 2 -terminal and hinge regions and confirmed that a mutation at Ser-650 has relatively little effect on AR transcriptional activity (28,35). We also found that mutations at other previously reported phosphorylation sites in the region, including a recently reported Src-dependent tyrosine phosphorylation site Tyr-534 in the AR NH 2 -terminal region close to the DNA binding domain (41), did not diminish AR transcriptional activity in CWR-R1 cells in response to DHT with or without EGF. The relative lack of functional effects of mutations at most of the previously reported AR phosphorylation sites left unanswered the question of the significance of AR phosphorylation, particularly in prostate cancer cells where growth factor and mitogen signaling are increased.
Our studies indicate direct effects of EGF signaling on AR transcriptional activity through Ser-515 in the NH 2 -terminal region and Ser-578 in the DNA binding domain. MAP kinase phosphorylation site Ser-515 is positioned near the DNA binding domain (Fig. 10A) carboxyl-terminal to activation function-1 transactivation domain residues 142-337 required for androgen-dependent AR transcriptional activity (1,42). AR Ser-515 is closer to tau-5 residues 360 -485 (43), a transcriptional activation region whose activity is apparent in the AR NH 2 -terminal and DNA binding domain fragment that lacks the ligand binding domain (1,42).
MAP kinase signaling was previously implicated in androgendependent AR transcriptional activity (13,17,44). In vitro MAP kinase assays suggested phosphorylation of AR-36 -643, an AR NH 2 -terminal and DNA binding domain fragment, in response to HER2/neu signaling (11). Although an AR S515A mutation reduced the signaling effects of HER2/neu, additional evidence for phosphorylation at this site was not reported. Here we show that EGF signaling slowed the gel electrophoretic migration of an AR NH 2 -terminal-DNA binding domain-hinge region fragment, an effect that was eliminated by treatment with -phosphatase. The EGF-dependent increase in AR transcriptional activity was linked to phosphorylation at Ser-515 and Ser-578.
MAP kinase consensus site mutation S515A reduced AR transcriptional activity slightly but did not eliminate the AR transcriptional response to EGF in the absence and presence of androgen. Inhibition of Ser-578 phosphorylation by an S578A mutation increased phosphorylation at Ser-515, which provided evidence that AR phosphorylation at Ser-515 is linked to phosphorylation at Ser-578.
Indeed, EGF-dependent phosphorylation at AR Ser-578 appears to be required for EGF stimulation of AR transcriptional activity. Ser-578 is positioned in the first zinc module of the AR DNA binding domain (Fig. 10B) within the P-box. Response element DNA binding specificity distinguished by the glucocorticoid receptor (GR) and estrogen receptor is associated with three P-box residues, GSXXV (45,46). Human AR P-box 577 GSCKV 581 contains Ser-578 and is common to GR and the progesterone and mineralocorticoid receptors, each of which recognizes the GGTACANNNTGTTCT (N is any nucleoside) consensus response element (47)(48)(49). P-box sequence within this subfamily distinguishes TGTTCT halfsite sequence from TGACCT estrogen response element consensus sequence (46). Within the AR receptor subgroup, DNA response element binding specificity was attributed to residues in the second zinc module and the carboxyl-terminal extension in the hinge region (50,51).
Crystal structures of the AR DNA binding domain-androgen response element DNA complex indicate that human AR Ser-578 lies within the first ␣-helix that directly contacts the major groove of the DNA (Fig. 10C) (52). Human AR Ser-578 corresponds to human GR Ser-440. When GR Ser-440 was changed to glycine to mimic Gly-204 in human ER or to alanine, GR DNA binding specificity was reduced. Based on these studies, the AR Ser-578 hydroxyl group is predicted to modulate DNA binding specificity through a hydrogen bond network extending to Arg-608 in the second zinc module loop containing AR D-box 596 ASRND 600 (Fig. 10B) (53). Unlike the common P-box sequence shared by AR, GR, progesterone, and mineralocorticoid receptors, the AR D-box, thought to be involved in receptor dimerization and half site spacing recognition, differs from the AGRND D-box common to other members of this subgroup of steroid receptors (45,54). AR Arg-608 corresponds to Arg-470 in human GR, for which a lysine substitution was predicted to alter DNA binding specificity by changing the hydrogen bonding pattern (53). The importance of AR Arg-608, which contacts the DNA phosphate backbone and is conserved in the steroid receptor family, is supported by the naturally occurring R608K mutation that causes partial androgen insensitivity.
A naturally occurring AR S578T mutation also causes grade 5 partial androgen insensitivity, where complete androgen insensitivity (grade 6/7) is associated with an external female phenotype in a genetic male (55,56). The S578T mutation demonstrates the in vivo importance of Ser-578 in AR function. The partial response of recombinant AR-S578T to elevated androgen levels in a COS cell transcriptional assay (55) indicates some retention of DNA binding activity. The extent of phosphorylation at residue 578 may be diminished by threonine at this position and phospho-Ser-578 or phospho-Thr-578 might alter AR interaction with DNA (Fig. 10C) or associated proteins such as Ku-70/80. Rapid release and rebinding of steroid receptors to DNA in a dynamic hit-and-run model has revealed the transient nature of steroid receptor DNA binding required for activation of transcription (57,58). Similar transient interactions were reported for AR binding to androgen response element DNA (59). EGF-dependent phosphorylation at AR Ser-578 may regulate AR association and dissociation from DNA and the magnitude of the transcriptional response.
We have provided evidence for PKC phosphorylation at AR Ser-578. Our results support previous evidence that phosphorylation by PKC in the DNA binding domain modulates nuclear receptor localization. PKC was shown to phosphorylate highly conserved Ser-78 between the two zinc modules in the DNA binding domain of hepatocyte nuclear factor-4␣ and at a corresponding position in retinoic acid receptor-␣, retinoid X receptor-␣, and thyroid hormone receptor-␤ (60). Hepatocyte nuclear factor-4␣ Ser-78 is conserved through most of the nuclear receptor superfamily, including Ser-212 in human estrogen receptor-␣. However, the corresponding residue in the AR subgroup of steroid receptors is alanine and is Ala-586 in human AR. PKC phosphorylation at Ser-78 in the DNA binding domain of hepatocyte nuclear factor-4␣ and other nuclear receptors regulates nuclear localization (60). Hepatocyte nuclear factor-4␣-S78A bound DNA, but the S78D phosphomimetic did not. The AR phosphomimetic S578D retained greater transcriptional activity than AR-S578A but less than wild-type AR (data not shown), and like wild-type AR, the S578D mutant was distributed in both the cytoplasm and nucleus. AR-S578D might be expected to bind less Ku-70/80, which could interfere with the dynamic model of steroid receptor binding to DNA.
The results raise the possibility that phosphorylation by PKC at conserved serine residues in the first zinc module of the DNA binding domain regulates DNA binding for the entire nuclear receptor family. Phosphorylation within the DNA binding domain may be a common regulatory mechanism that controls nuclear-cytoplasmic shuttling and DNA binding required for differential gene regulation.
AR Nuclear-Cytoplasmic Shuttling and DNA Binding Specificity-EGF-dependent AR transcriptional activity mediated by PKC-dependent phosphorylation at AR Ser-578 is linked to AR nuclear-cytoplasmic shuttling. Based on results with an AR NH 2 -terminal, DNA binding, and hinge region fragment, where the S578A mutant was exclusively nuclear, EGF-dependent AR phosphorylation at Ser-515 and Ser-578 modulates AR nuclear retention. In addition, AR coimmunoprecipitates with Ku-70/80, a DNA binding protein complex that regulates DNA-PK activity and other transcription factors (61). The greater nuclear retention of AR-(507-660)-S578A compared with wild type was associated with increased interaction with Ku-70/80. PKC-mediated AR phosphorylation at Ser-578 appears to regulate the AR interaction with Ku-70/80 and modulate AR nuclear-cytoplasmic shuttling and DNA binding. Our studies are in agreement with previous evidence that steroid receptor phosphorylation is linked to nuclear-cytoplasmic shuttling (62).
In agreement with evidence that AR-S578A interacts to a greater extent with Ku-70/80, full-length AR-S578A did not activate MMTV-Luc in Ishikawa cells (data not shown) even though AR-S578A transcriptional activity was similar to wild type in Ishikawa cells using the PSA-Enh-Luc reporter, which lacks a negative response element (NRE) sequence present in the MMTV promoter-enhancer. Ku-80 represses GR transactivation through high affinity, sequence-specific binding to double-stranded negative response element 1 at Ϫ394 to Ϫ381 in the MMTV long terminal repeat (63,64). Repression of GR transactivation of the MMTV promoter correlated with recruitment of Ku-70/80 to negative response element 1 (64,65,66).
Loss of AR-S578A transactivation of MMTV-Luc did not result from inhibition of AR binding to DNA. In the context of the AR NH 2 -terminal and DNA binding domain constitutively active fragment, AR-S578A retains wild-type activity with MMTV-Luc (data not shown). This is consistent with a previous study suggesting that Ku-70/80 interacts preferentially with the AR ligand binding domain (33) and provides further evidence that the effect of the S578A mutation is to increase AR interaction with Ku-70/80. Our studies suggest that a stronger interaction between AR and Ku-70/80 is associated with loss of AR activation of MMTV. In addition, the results support the concept that EGF-dependent phosphorylation at Ser-578 modulates AR transactivation through its interaction with Ku-70/80. EGF-dependent phosphorylation sites Ser-515 and Ser-578 in AR differ from the GR DNA-PK phosphorylation site that was linked to GR association with Ku-70/80. Human GR hinge region DNA-PK phosphorylation site Ser-508 implicated in GR nuclear retention (63,66,67) is positioned close to human GR nuclear targeting residues 479 -498 and is displaced in position by two residues to the corresponding human AR hinge residue Ser-650 near AR nuclear targeting residues 617-633 (22,68). We and others have shown that a mutation at Ser-650 has relatively little effect on AR transcriptional activity (28,35). However, human AR has a DNA-PK consensus phosphorylation site 656 TQ 657 in the hinge region, and inhibition of DNA-PK was reported to reduce AR phosphorylation and nuclear export (69). Given that AR interacts with Ku-70/80, an interaction that is enhanced by the S578A mutation, and that Ku-70/80 has additional functions independent of DNA-PK (65), Ku-70/80 may regulate AR independent of DNA-PK. Ku-70/80 is reported to have DNA helicase activity (70), which may more directly influence AR transcriptional activity.
AR Function in Castration-recurrent Prostate Cancer-AR is an important transcriptional activator in castration-recurrent prostate cancer growth that follows a period of remission in response to androgen deprivation therapy. The AR gene is amplified in approximately one-third of prostate cancers (71). AR somatic gene mutations in prostate cancer can increase AR transactivation by androgens and other ligands. However, although AR mutations are relatively common in established prostate cancer cell lines, they are infrequent in prostate cancer tissue specimens and cannot account for the high incidence of castration-recurrent prostate cancer growth after androgen deprivation therapy. AR somatic mutations in prostate cancer, such as AR-H874Y in the CWR22 human prostate xenograft and the derived CWR-R1 prostate cancer cell line used in the present study (6,15), tend to increase AR sensitivity to liganddependent transactivation through more efficient recruitment of SRC/p160 coactivators by activation domain-2 in the ligand binding domain (72). In contrast, ligand-independent AR activation may be independent of activation domain-2 in the ligand binding domain (73), mediated instead by growth factor and mitogen downstream signaling mechanisms (74). This is supported by the inability of flutamide to inhibit castration-recurrent prostate cancer cell growth (75,76).
Chromatin studies support AR function in cell proliferation in castration-recurrent prostate cancer in the absence of androgen (77)(78)(79)(80)(81). Adeno-associated viral siRNA knockdown of AR diminished both DHT and EGF-stimulated CWR-R1 prostate cancer cell growth. However, residual levels of AR in the AR-siRNA-scAAV-treated cells appear to be sufficient to mediate prostate cancer cell growth in response to the synergistic effects of DHT and EGF, a finding relevant to clinical prostate cancer since androgens are present in castration-recurrent prostate cancer tissue (8). In addition, EGF was shown to induce sitespecific phosphorylation and monoubiquitinylation of AR coregulator MAGE-11 required to interact with AR and increase transcriptional activity in the absence and presence of androgen (16). Thus, growth factor and mitogen signaling through multiple mechanisms that involve AR may account for the relapse of prostate cancer growth.