Zinc Dyshomeostasis Is Linked with the Loss of Mucolipidosis IV-associated TRPML1 Ion Channel*

Chelatable zinc is important in brain function, and its homeostasis is maintained to prevent cytotoxic overload. However, certain pathologic events result in intracellular zinc accumulation in lysosomes and mitochondria. Abnormal lysosomes and mitochondria are common features of the human lysosomal storage disorder known as mucolipidosis IV (MLIV). MLIV is caused by the loss of TRPML1 ion channel function. MLIV cells develop large hyperacidic lysosomes, membranous vacuoles, mitochondrial fragmentation, and autophagic dysfunction. Here, we observed that RNA interference of mucolipin-1 gene (TRPML1) in HEK-293 cells mimics the MLIV cell phenotype consisting of large lysosomes and membranous vacuoles that accumulate chelatable zinc. To show that abnormal chelatable zinc levels are indeed correlated with MLIV pathology, we quantified its concentration in cultured MLIV patient fibroblast and control cells with a spectrofluorometer using N-(6-methoxy-8-quinolyl)-p-toluene sulfonamide fluorochrome. We found a significant increase of chelatable zinc levels in MLIV cells but not in control cells. Furthermore, we quantified various metal isotopes in whole brain tissue of TRPML1−/− null mice and wild-type littermates using inductively coupled plasma mass spectrometry and observed that the zinc-66 isotope is markedly elevated in the brain of TRPML1−/− mice when compared with controls. In conclusion, we show for the first time that the loss of TRPML1 function results in intracellular chelatable zinc dyshomeostasis. We propose that chelatable zinc accumulation in large lysosomes and membranous vacuoles may contribute to the pathogenesis of the disease and progressive cell degeneration in MLIV patients.

Zinc (Zn 2ϩ ) is a redox-inert yet crucial trace element for virtually all organisms. Zn 2ϩ is second to iron in biological trace metal abundance and is known to play an important role in human brain function (1,2). Some enzymes require Zn 2ϩ as co-factors, but many proteins use it for structural stability and thus are tightly bound (non-chelatable form) (3). A chelatable pool of Zn 2ϩ is present in cells, most notably in glutamatergic vesicles, and gets released at the neuronal synapse during normal and pathological states (4,5). Because high levels of chelatable Zn 2ϩ or any trace metals produce cellular and mitochondrial toxicity (1,6,7), it is imperative that Zn 2ϩ homeostasis is actively maintained inside and outside of the cells. Certain pathological events such as epilepsy, cerebral stroke, and traumatic brain injury result in uncontrolled release and accumulation of chelatable Zn 2ϩ in neurons (1,8) via voltage-gated or calcium-permeable ion channels (9). Furthermore, endosomes, autophagosomes, and lysosomes, which are critical organelles involved in the recycling and degradation of many proteins, are known compartments where chelatable Zn 2ϩ accumulates upon cellular perturbation (10 -12). Interestingly, chelatable Zn 2ϩ appears to mediate vacuolar formation in primary retinal cells exposed to ethambutol (an antituberculosis drug known for its ocular toxicity) (13,14) and autophagic vacuole formation in astrocytes upon exposure to hydrogen peroxide (12). Consequently, high levels of chelatable Zn 2ϩ accrue in ethambutol-induced vacuoles, which induce lysosomal membrane permeabilization and retinal cell death (13). The association of chelatable Zn 2ϩ in neurodegeneration led us to study its potential role in mucolipidosis IV (MLIV), 3 a developmental disorder that results in large hyperacidic lysosomes, membranous vacuole formation, autophagic dysfunction, and mitochondrial fragmentation (15)(16)(17)(18). MLIV is an autosomal, recessive disease that generally results in brain and cognitive dysfunction, corneal clouding, and retinal cell degeneration leading to blindness, anemia, and achlorhydria (lack of gastric acid production in the stomach) (19). It is caused by the loss of transient receptor potential mucolipin-1 (TRPML1) ion channel function. TRPML1 is widely expressed in tissues and organs. It is a lysosomal membrane protein believed to play a role in endosomallysosomal interaction, lysosomal pH stability, lysosome maturation, lipid metabolism, and autophagy (16 -18, 20, 21). TRPML1 is an inwardly rectifying, non-selective cation channel that is permeable to calcium (Ca 2ϩ ) and other divalent trace metals such as Zn 2ϩ , iron (Fe 2ϩ ), and manganese (Mn 2ϩ ) (22)(23)(24). It is noteworthy that histochemical Prussian blue staining shows that chelatable Fe 2ϩ levels in the cytosol of MLIV fibroblasts appear to be reduced, whereas levels in the lysosomes appear elevated when compared with control cells (22). These findings suggest that an impairment in chelatable Fe 2ϩ permeability may be causal to MLIV disease (22). Notwithstanding, we hypothesize that chelatable Zn 2ϩ , and not Fe 2ϩ , could be the * This work was supported, in whole or in part, by National Institutes of Health mitigating factor in the cellular pathology and progressive cell degeneration in MLIV disease. In this report, we investigated whether chelatable Zn 2ϩ invariably accumulates within lysosomes of cells following RNA interference of TRPML1 and whether its concentrations are significantly perturbed upon the loss of TRPML1 ion channel function in MLIV patient fibroblast cells, as well as in the brain tissue of TRPML1 Ϫ/Ϫ knockout mice.
RNA Interference (RNAi)-We validated the effect of a short hairpin RNA vector targeting human mucolipin-1 gene (TRPML1 shRNA-1208) as described previously (28,29) and human mucolipin-2 (TRPML2) gene (supplemental Fig. S1). A scrambled shRNA vector control (OriGene Technologies) was also tested and validated (supplemental Fig. S1). Human embryonic kidney (HEK)-293 cells were transfected with TRPML1 and -2 and scrambled shRNA vectors (2 g) and processed for confocal or electron microscopy (EM) 48 h after transfection. Standard EM technique was performed on TRPML1 RNAitreated and untreated cells as described (30). For confocal microscopy, the cells were washed with phosphate-buffered saline (PBS), incubated for 30 -60 min with either FluoZin-3 or Newport Green DCF (1 M; Invitrogen) to detect chelatable Zn 2ϩ , and incubated with either LysoTracker TM Red DND-99 or Blue DND-22 (0.5 nM; Invitrogen) to detect lysosomes or acidic compartments. LysoTracker Blue DND-22 was used in experiments using TRPML2 and scrambled control shRNA because both plasmids were tagged with a red fluorescent protein marker to monitor expression. The cells were then washed twice with PBS, mounted on slides with ProLong TM Gold antifade reagent (Invitrogen), and imaged without fixation. The red channel was used to show the LysoTracker Blue DND-22 micrographs of TRPML2 RNAi and scrambled RNAi. In other experiments, DAPI was added to stain the cell nucleus.
Primary Cultures of MLIV and Control Fibroblasts-Two MLIV patient skin fibroblast cells (GM 02048 and GM 02527) and two control skin fibroblast cells (GM 00408 and GM 03440) were purchased from Coriell Cell Repositories (Camden, NJ) and cultured in triplicates according to their recommendations. The cells were washed twice with PBS, incubated with TSQ (1 mM) for 30 -60 min, washed twice with PBS, and assayed using a BioTek Synergy 2 spectrofluorometer. The relative fluorescence units obtained from Zn 2ϩ -TSQ chelates were normalized by total cell numbers per trial using the Cell TiterGlo viability luminescence kit (Promega) to control for any assay variations. Brain Metal Isotope Analysis-We obtained brain tissues (1 gm) from TRPML1 Ϫ/Ϫ knock-out (KO) mice (n ϭ 4), and wildtype (WT) littermate controls (n ϭ 3). The frozen brain samples were sent to the California State University Long Beach, Institute for Integrated Research, Materials, Environments, and Society (IIRMES) Core facility for inductively coupled plasma mass spectrometry (ICP-MS) analysis of various metal isotopes such as sodium-23, calcium-43, calcium-44, magnesium-24, aluminum-27, chromium-52, manganese-55, iron-54, iron-57, copper-63, copper-65, and zinc-66. The samples were normalized by total protein content prior to ICP-MS analysis to control for any handling errors introduced during ICP-MS analysis. Data are represented as means Ϯ S.E. and analyzed for statistical significance using Student's t test (two-tailed, p Ͻ 0.05).

RESULTS
To model MLIV in vitro, we used RNAi to knock down endogenous TRPML1 protein levels in HEK-293 cells. Electron microscopy showed that TRPML1 RNAi exhibited electron-dense, large membranous vacuoles that were characteristic of MLIV cell phenotype (Fig. 1A). RNAi knockdown of cells that normally express TRPML1 has been reported to produce such cell phenotype by 48 -72 h (21,31). Interestingly, phagophore-like membrane structures were also apparent near dense vacuolar structures (horseshoe or half-circle shapes, Fig. 1A, arrows); phagophores are double membrane structures that initiate autophagy. Fluorescence microscopy showed that lysosomes in these TRPML1 RNAi-treated cells were more pronounced and much larger in comparison with those of untreated control cells (Fig.  1B). Indeed, both non-RNAitreated control and scrambled RNAi-treated control cells had mostly smaller lysosomes with sporadic FluoZin-3 positive Zn 2ϩ fluorescence (Fig. 2, A and B), or Newport Green DCF (supplemental Fig.  S2). The TRPML2 RNAi-treated cells occasionally had large Lyso-Tracker-positive lysosomes that cofluoresced with FluoZin-3 (Fig. 2C). However, the size of the lysosomes in these TRPML2 RNAi-treated cells appeared similar to untreated and scrambled RNAi control cells, whereas in TRPML1 RNAi-treated cells, both lysosomes and vacuolar structures that were positive for Lyso-Tracker were brightly fluorescent for chelatable Zn 2ϩ as evidenced by either FluoZin-3 (Fig. 2, D and E) or Newport Green DCF stain (supplemental Fig. S2). No fluorescence was observed using the non-permeable FluoZin-3 control dye (not shown). Noteworthy is a phagophore-like structure that can be seen from TRPML1 RNAi-treated cells co-fluorescing with LysoTracker and FluoZin-3 (Fig. 2E).
To determine whether chelatable Zn 2ϩ levels were abnormal in TRPML1 RNAi-treated HEK-293 cells when compared with controls, we performed TSQ spectrofluorometric analysis of the samples. We observed an increase of relative Zn 2ϩ -TSQ fluorescence in TRPML1 RNAi-treated cells but not with untreated, scrambled RNAi, and TRPML2 RNAi controls (not shown). To verify whether this observation was relevant within the context of MLIV pathology, we quantified chelatable Zn 2ϩ concentrations in primary cultured MLIV skin fibroblast and non-patient control cells. Spectrofluorometric quantification revealed a significant 2-3-fold increase in relative Zn 2ϩ -TSQ fluorescence in fibroblasts of MLIV patients (GM 02048 and GM 02527) when compared with control fibroblast cells (GM 03440 and GM 00408) (Fig. 3). Because neurons are affected by MLIV, our in vitro findings prompted us to further study Zn 2ϩ levels using whole brain tissues of TRPML1 Ϫ/Ϫ KO mice and WT littermates. Indeed, ICP-MS analysis showed a small but significant increase in the concentration of zinc isotope-66 ( 66 Zn) from TRPML1 Ϫ/Ϫ KO mouse brain tissues (11.16 Ϯ 0.5 g/g; p Ͻ 0.05) but not from WT littermate controls (9.34 Ϯ 0.4 g/g). No other metal isotopes analyzed were found to be significantly different in these samples (not shown).

DISCUSSION
Our study shows that the loss of TRPML1 function leads to significant accumulation of chelatable Zn 2ϩ in large lysosomes and vacuolar structures. Chelatable Zn 2ϩ fluorescence is observed within lysosomal lumen and vacuolar lumen. These results intriguingly resemble what has been reported as a Zn 2ϩ buildup in ethambutol-induced vacuoles in retinal cells (13,14) and hydrogen peroxide-induced autophagic vacuoles in astrocytes (12). Chelatable Zn 2ϩ appears to mediate autophagic and lysosomal vacuole formation in these aforementioned cells because Zn 2ϩ chelation reduces such an effect, but whether it could also be involved in vacuolar formation upon the loss of TRPML1 function remains to be determined. Nevertheless, significant chelatable Zn 2ϩ elevation was evident in MLIV patient fibroblast cells, as well as in the whole brain tissue of TRPML1 Ϫ/Ϫ KO mice. It would have been ideal to study the levels of zinc using human postmortem MLIV brain tissue in addition to the mouse model, but unfortunately, such precious tissue was not available for our investigation.
We found a 2-3-fold increase in chelatable Zn 2ϩ levels using spectrofluorometry, whereas our ICP-MS data showed a modest increase in 66 Zn isotope levels. The difference in magnitude can be explained by methodological differences; the former measured chelatable levels, whereas the latter measured total tissue zinc isotope contents. Moreover, diet and drinking water in rodents have been shown to influence brain zinc levels (32,33), making whole brain tissue analysis of trace metals from rodents more prone to variability in comparison with cultured cells where known amounts of trace metals are found in media or buffers. Nevertheless, we clearly established that abnormal Zn 2ϩ metabolism is present in MLIV cells. Specifically, the loss of TRPML1 appears to increase the amount of chelatable Zn 2ϩ in the lysosomes but not in untreated control, scrambled RNAitreated, and TRPML2 RNAi-treated cells. Although RNAi-mediated TRPML2 knockdown has been previously shown to exhibit similar EM-dense membranous structure in HeLa cells (31), our results showed only a few large lysosomes that also co-localized with chelatable Zn 2ϩ fluorescence despite the fact that the TRPML2 shRNA vector efficiently knocked down heterologously overexpressed TRPML2 protein. This observation suggests that the loss or disruption of TRPML1 protein function makes cells highly vulnerable to intracellular chelatable Zn 2ϩ dyshomeostasis.
Recently, histochemical evidence using Prussian blue stain showed that chelatable Fe 2ϩ levels in the cytosol were reduced, whereas its levels in lysosomes were elevated in MLIV fibroblast cells with distinct mucolipin-1 gene mutation (22). However, we did not observe a significant change in iron isotope levels from TRPML1 Ϫ/Ϫ KO mouse brain samples, but rather found that 66 Zn levels were significantly different. Furthermore, colorimetric measurement of total Fe 2ϩ levels in TRPML1 RNAitreated HEK-293 cells did not show significant changes when compared with untreated control. 4 It is possible that differences in the techniques may explain such discrepancies; the previous study looked at chelatable Fe 2ϩ concentrations in MLIV fibroblast using Prussian blue stain (22), whereas our collaborators 4 quantified total Fe 2ϩ concentrations of TRPML1 RNAi-treated cultured cells using the wet-ash method, and we quantified levels of iron isotopes from TRPML1 Ϫ/Ϫ KO mice using ICP-MS.
The known cellular and mitochondrial toxicities of chelatable Zn 2ϩ (1,6,7) suggest that our findings could be relevant to cell death and progressive degeneration in MLIV. Likewise, high levels of dietary zinc have been shown to induce iron deficiency and anemia in rodents (34). Because anemia is a clinical feature of MLIV, it would be interesting to know whether 4 C. Morrison and M. Linder, personal communication. changes in systemic and/or tissue zinc levels of MLIV patients correlate with iron deficiency and anemia.
In conclusion, we showed for the first time that Zn 2ϩ metabolism is impaired in cells lacking a functional TRPML1 ion channel. These findings support our hypothesis that TRPML1 dysfunction leads to changes in concentrations of chelatable Zn 2ϩ pool, but not Fe 2ϩ , as evidenced by both cellular and in vivo models of MLIV disease. Future research should investigate whether chelatable Zn 2ϩ elevation contributes to cell death and progressive degeneration of neuronal and retinal cells using the mouse model for MLIV and whether the use of zinc-specific chelator could reverse such negative effects.