Identification and Characterization of Oxalate Oxidoreductase, a Novel Thiamine Pyrophosphate-dependent 2-Oxoacid Oxidoreductase That Enables Anaerobic Growth on Oxalate*

Moorella thermoacetica is an anaerobic acetogen, a class of bacteria that is found in the soil, the animal gastrointestinal tract, and the rumen. This organism engages the Wood-Ljungdahl pathway of anaerobic CO2 fixation for heterotrophic or autotrophic growth. This paper describes a novel enzyme, oxalate oxidoreductase (OOR), that enables M. thermoacetica to grow on oxalate, which is produced in soil and is a common component of kidney stones. Exposure to oxalate leads to the induction of three proteins that are subunits of OOR, which oxidizes oxalate coupled to the production of two electrons and CO2 or bicarbonate. Like other members of the 2-oxoacid:ferredoxin oxidoreductase family, OOR contains thiamine pyrophosphate and three [Fe4S4] clusters. However, unlike previously characterized members of this family, OOR does not use coenzyme A as a substrate. Oxalate is oxidized with a kcat of 0.09 s−1 and a Km of 58 μm at pH 8. OOR also oxidizes a few other 2-oxoacids (which do not induce OOR) also without any requirement for CoA. The enzyme transfers its reducing equivalents to a broad range of electron acceptors, including ferredoxin and the nickel-dependent carbon monoxide dehydrogenase. In conjunction with the well characterized Wood-Ljungdahl pathway, OOR should be sufficient for oxalate metabolism by M. thermoacetica, and it constitutes a novel pathway for oxalate metabolism.

Moorella thermoacetica is a strictly anaerobic Gram-positive acetogenic bacterium. Acetogens are commonly found in the soil, animal gastrointestinal tract, and the rumen and grow heterotrophically or autotrophically on many different electron donors. Electrons from these substrates are used to reduce CO 2 to acetate by the Wood-Ljungdahl pathway. During growth by this pathway, acetate and cell mass are the only growth products, and electron-rich growth substrates like glucose are converted stoichiometrically to acetate; therefore, M. thermoacetica is called a homoacetogen. M. thermoacetica can use other electron acceptors (e.g. nitrate, nitrite, thiosulfate, and dimethyl sulfoxide). Under most conditions, nitrate reduction occurs preferentially to CO 2 reduction, and nitrate has been shown to repress autotrophic growth (1,2). However, oxalate is unique in its properties as an electron donor by M. thermoacetica for acetogenic growth. When both nitrate and CO 2 are provided to cells growing on oxalate, CO 2 is reduced to acetate during exponential growth phase, and nitrate is only used as the electron acceptor during stationary phase (3). Oxalate is the only substrate with which M. thermoacetica has been shown to reduce CO 2 instead of nitrate when both are present.
Oxalate (C 2 O 4 2Ϫ ) is the most oxidized two-carbon compound. It is made in high concentrations by some plants and fungi and can reach high micromolar concentrations in soil (4). Oxalate is toxic to mammals but is metabolized by many bacteria and plants by various pathways. In Oxalobacter formigenes, oxalate is first activated to oxalyl-CoA and then decarboxylated, giving formyl-CoA and CO 2 . Formyl-CoA transferase then exchanges the formyl group for an oxalyl-group on CoA, thus producing formate and regenerating oxalyl-CoA. Energy for growth on oxalate in O. formigenes results from a formate-oxalate antiporter, which generates an electrochemical transmembrane gradient for ATP synthesis (5,6), so most formate produced is excreted rather than oxidized (7). Other organisms, such as Cupriavidus oxalaticus, also use oxalyl-CoA decarboxylase to metabolize oxalate but use formate dehydrogenase to generate NADH with the electrons derived from oxalate (8,9). Oxalyl-CoA decarboxylase is a TPP-dependent enzyme with distant homology to yeast pyruvate decarboxylases (10). The M. thermoacetica genome has sequences with low homology to oxalyl-CoA decarboxylase but no homolog of formyl-CoA transferase. In fungi and in some bacteria, including Bacillus subtilis, oxalate can also be metabolized by oxalate decarboxylase, a manganese-containing cupin family protein, to generate formate and CO 2 (11). This reaction requires O 2 . Another Mn 2ϩ -cupin called germin that is found in plants catalyzes the oxidative decarboxylation of oxalate in the reaction C 2 O 4 2Ϫ ϩ 2H ϩ ϩ O 2 3 2CO 2 ϩ H 2 O 2 (12). No enzyme that catalyzes anaerobic oxalate oxidation has been reported previously.

C 2 O 4
Ϫ 2 ϩ 5 H 2 O 3 CH 3 COO Ϫ ϩ 6 HCO 3 Ϫ ϩ OH Ϫ ͑⌬G ϭ Ϫ 41.4 kJ/mol oxalate͒ REACTION 1 Two proteins (33 and 42 kDa) were identified as being induced when M. thermoacetica was exposed to oxalate (14). Similarly, Daniel et al. (14) found that oxalate-grown cells metabolize oxalate much more quickly than cells that had been grown on glucose, which is consistent with an oxalate induction mechanism (15). They also showed that cell extracts from oxalate-grown cells could catalyze oxalate-dependent benzyl viologen reduction, that this activity was only slightly stimulated by coenzyme A, and that the electron acceptor specificity of oxalate oxidation was different than that of formate oxidation. They concluded that M. thermoacetica catabolizes oxalate by a CoA-independent mechanism that does not use formate as an intermediate. This paper describes the characterization of the oxalateinduced enzyme, oxalate oxidoreductase (OOR) 2 and demonstrates that it, unlike all previously characterized members of the thiamine pyrophosphate (TPP)-dependent 2-oxoacid: ferredoxin oxidoreductase family, does not require coenzyme A. Coupling OOR to the Wood-Ljungdahl pathway allows anaerobic bacteria to generate energy by converting oxalate to acetyl-CoA.

EXPERIMENTAL PROCEDURES
Culture Media and Growth Conditions-M. thermoacetica ATCC 39073 was grown at 55°C in stoppered, crimp-sealed 120-ml serum bottles pressurized with pure CO 2 and containing 100 ml of medium, or in a 10-liter fermentor that was continually bubbled with CO 2 during growth. The medium was modified from the "undefined medium" of Lundie and Drake (16) (16), and 10 ml/liter reducing solution (36 g/liter each of Na 2 S⅐9H 2 O and cysteine-HCl). Cells were harvested during exponential growth by centrifugation under CO 2 or N 2 and were stored at Ϫ80°C until use.
Two-dimensional Gel Electrophoresis-For two-dimensional gel electrophoresis, proteins were extracted with phenol as described (17), except that instead of tissue grinding, sonication was used to break the cells and, after precipitation of proteins in methanol, the protein pellet was washed twice with ice-cold acetone (80% in water) and once with ice-cold ethanol (70% in water). After washing, the protein pellets were suspended in 8 M urea, 2 M thiourea, 2% (w/v) CHAPS, 2% (w/v) Triton X-100, and 50 mM dithiothreitol (DTT) to ϳ1 g/l protein. Samples (150 l) were supplemented with Bio-Lyte ampholytes (Bio-Rad) and loaded on 7-cm Bio-Rad isoelectric focusing gel strips (gradient from pH 5 to 8). The gels were subjected to a 12-h rehydration at 50 V, followed by focusing at 250 V for 15 min and then at 4000 V for 35,000 V-h. After isoelectric focusing, the isoelectric focusing gel strips were soaked in 6 M urea, 2% (w/v) SDS, 0.375 M Tris-HCl, pH 8.8, 20% (w/v) glycerol, and 20 mg/ml DTT for 15 min, followed by incubation for 15 min in the same buffer but with 25 mg/ml iodoacetamide instead of DTT. Strips were rinsed in running buffer before loading them at the top of 12.5% acrylamide gels for SDS-PAGE. After SDS-PAGE, proteins were stained with Coomassie Blue. Spots were cut from the gels, and proteins were identified by mass spectrometry at the University of Michigan Protein Structure Facility. A blank spot of each gel was also taken and processed. The samples were subjected to in-gel trypsin digestion. As a control, BSA was run on a separate gel and subjected to the same digestion and mass spectrometry procedure. LC-MS/MS was performed on a Q-TOF Premier mass spectrometer. Protein Lynx Global Server and Mascot search engines were used to search the SwissProt and NCBI databases.
Purification of OOR-Protein was purified from M. thermoacetica cells grown in the fermentor on glucose and oxalate. All purification steps and subsequent enzymatic manipulations were done in a Vacuum Atmospheres (Hawthorne, CA) anaerobic chamber maintained at Յ4 ppm O 2 . Cells were suspended in Buffer A (50 mM Tris-HCl, 2 mM MgCl 2 , 2 mM DTT, 1 mM TPP, pH 7.9) with 0.25 mg/ml lysozyme and 0.2 mM phenylmethanesulfonyl fluoride, sonicated, and centrifuged at 4°C at 100,000 ϫ g for 1 h. The supernatant was loaded on a 5 ϫ 25-cm DEAE-cellulose column, and proteins were eluted with a gradient from 0.1 to 0.5 M in Buffer A. The 0.3 M fraction was diluted to 0.075 M NaCl with Buffer A and loaded on a 2.5 ϫ 25-cm red agarose (Sigma-Aldrich) column. OOR did not bind to this column, so 0.85 M ammonium sulfate was added to the flow-through and wash fractions, and these were loaded on a 2.5 ϫ 25-cm fast flow high substitution phenyl-Sepharose (GE Healthcare) column and eluted with a reverse linear gradient from 0.85 to 0 M ammonium sulfate and 10% glycerol in Buffer A. Oxalate oxidation activity eluted at around 0.45 M ammonium sulfate. Fractions containing OOR activity were pooled, concentrated, and exchanged into Buffer A using 30-kDa molecular mass cut-off centrifuge concentrators. The protein was loaded on a 2.5 ϫ 25-cm high performance Q-Sepharose column (Sigma-Aldrich) and eluted with a linear gradient from 0 to 0.7 M NaCl in Buffer A. OOR activity eluted at 0.47 M NaCl. Fractions containing OOR activity were pooled and exchanged into 50 mM Tris-HCl, pH 7.9, and 2 mM DTT. OOR was stored in 50 mM Tris-HCl, pH 7.9, and 2 mM DTT at a concentration of 210 M, and dilutions of this protein stock were 2  used for all subsequent experiments unless described otherwise. Enzyme Assays-OOR activity was measured in 50 mM Tris-HCl, 2 mM DTT, pH 7.9. Assays at 25°C were done in a Vacuum Atmospheres anaerobic chamber maintained at Յ4 ppm O 2 , using a UV-visible spectrophotometer from Ocean Optics (Dunedin, FL). Assays at 40 or 55°C were performed in stoppered cuvettes flushed with N 2 gas, in an OLIS (Bogart, GA)-modified Cary-14 spectrophotometer. For steady-state assays performed with saturating substrate concentrations, 1 mM sodium oxalate and 10 mM methyl viologen were used, and the reduction of methyl viologen was followed at 578 nm (⑀ 578 ϭ 9.7 mM Ϫ1 cm Ϫ1 ). In assays using ferredoxin (⌬⑀ 420 ϭ 7.5 mM Ϫ1 cm Ϫ1 ), horse heart cytochrome c (⌬⑀ 553 ϭ 19.4 mM Ϫ1 cm Ϫ1 ), NAD ϩ or NADP ϩ (⑀ 340 ϭ 6.22 mM Ϫ1 cm Ϫ1 ), FMN (⑀ 450 ϭ 12.2 mM Ϫ1 cm Ϫ1 ), FAD (⑀ 450 ϭ 11.3 mM Ϫ1 cm Ϫ1 ), or CO dehydrogenase (CODH), methyl viologen was omitted. The extinction coefficients for FMN and FAD are for two-electron reductions. The ⌬⑀ 320 for metronidazole was determined by titrating 100 M metronidazole with 20 M aliquots of oxalate, in the presence of 1.8 M OOR (⌬⑀ 320 ϭ 3.2 (mM reducing equivalent) Ϫ1 cm Ϫ1 ) at 320 nm. In all assays, calculations were based on the assumption that oxidation of 1 mol of oxalate produces 2 mol of electrons. CODH as an electron acceptor for OOR was assayed in 50 mM sodium phosphate, pH 7.0, with 1 mM oxalate, 20 M CODH/ acetyl-CoA synthase (ACS) from M. thermoacetica, and 50 M myoglobin in stoppered cuvettes that were flushed with 20% CO 2 , 80% N 2 (6.8 mM CO 2 in solution). The stock solution of myoglobin was prereduced by adding a stoichiometric amount of sodium dithionite. Formation of CO from CO 2 was measured as myoglobin-bound CO. At pH 7, the specific activity of CO 2 reduction by CODH using dithionite (instead of OOR) as a reductant was 4.7 mol min Ϫ1 mol CODH Ϫ1 . Formation of myoglobin-CO was monitored at 423 nm. The extinction coefficient, ⌬⑀ 423 ϭ 129 mM Ϫ1 cm Ϫ1 , was determined from the difference between the spectra of myoglobin-CO and dithionite-reduced myoglobin. The concentration of myoglobin used for these spectra was determined from extinction coefficients of 121 mM Ϫ1 cm Ϫ1 at 435 nm for ferrous myoglobin and 207 mM Ϫ1 cm Ϫ1 at 423 nm for myoglobin-CO (18).
For whole cell assays, cells were harvested from growing cultures, washed twice with 15 mM NaCl in 50 mM potassium phosphate, pH 7.0, and resuspended in stoppered serum bottles in fresh growth medium with 10 mM oxalate and 1 atmosphere of CO 2 . Aliquots were removed with a syringe and quenched in 1 M HCl, and oxalate concentrations were measured by HPLC on a 300 ϫ 7.8 mm Bio-Rad Aminex HPX-87H column with a mobile phase of 0.008 N H 2 SO 4 in a Beckman Coulter (Brea, CA) System Gold HPLC with diode array UV-visible detector. Oxalate was detected by its absorbance at 210 nm. Concentrations were determined by comparison with oxalate standards prepared in the medium used for the assays. Oxalate concentrations between 0.25 and 10 mM could be reliably determined by this assay. HPLC-based assays with purified protein or cell extracts were performed in the same way, except that 50 mM Tris-HCl, pH 8.0, with 10 mM oxalate and 10 mM methyl viologen (under an N 2 atmosphere) was used instead of growth medium, and oxalate standards were prepared in the assay buffer.
The pH dependence of OOR activity was determined by measuring activity with 1 mM oxalate and 10 mM methyl viologen in 50 mM buffer containing 2 mM DTT. Buffers were made by mixing 50 mM solutions of conjugate acid and base in different proportions, and the pH of each buffer and DTT solution was measured before starting each assay. The buffers used were MES (pK a ϭ 6.02, used from pH 5.0 to 7.1), sodium phosphate (pK a ϭ 6.82, used from pH 5.9 to 7.8), borate (pK a ϭ 8.94, used from pH 8.1 to 9.1), and N-cyclohexyl-3amino-propanesulfonic acid (pK a ϭ 9.42, used from pH 8.4 to 10.2).
UV-visible Spectroscopy-OOR was diluted to 4.1 M in 50 mM Tris-HCl, pH 7.9, and 2 mM DTT to measure the UVvisible spectrum of the as-isolated protein. The enzyme was reduced at 25°C by adding 100 M sodium oxalate. To measure the spectrum of the oxidized protein, 4.1 M OOR was mixed with 50 nM M. thermoacetica CODH/ACS in 50 mM potassium phosphate, pH 7.0. The cuvette containing this mixture was stoppered, and the headspace was flushed with 20% CO 2 , 80% N 2 . After 5 min of CO 2 exposure, the spectrum was recorded. In this reaction, electrons from OOR are transferred to CODH, which reduces the CO 2 to CO. Once OOR was fully oxidized, the spectrum did not change during another 1 h of CO 2 exposure.
EPR Spectroscopy-OOR was concentrated and diluted in 50 mM Tris-HCl, pH 8.0 (without DTT), to a concentration of 49 M. Comparison of the UV-visible spectrum of this protein with dithionite-reduced OOR showed that the protein was two-thirds reduced. Sodium oxalate (50 M, final) was added to completely reduce the protein. After reduction, different amounts of horse heart cytochrome c and 50 mM Tris-HCl, pH 8.0, were added to generate samples that were 39 M OOR and between 20 and 160 M cytochrome c. EPR spectra were collected at 9 K, and the parameters were as follows: receiver gain, 2 ϫ 10 2 ; modulation frequency, 100 kHz; modulation amplitude, 10 G; center field, 3450 or 3500 G; sweep width, 700 or 2000 G; microwave power, 0.129 milliwatt. The double integrals of the EPR signals were compared with that of a 1 mM copper(II) perchlorate standard to determine the number of spins per monomeric unit.
Size Exclusion Chromatography-A 90 ϫ 1.6-cm column of Superdex 200 resin (GE Healthcare) was equilibrated with an anaerobically prepared solution of 50 mM Tris-HCl, pH 8.1, with 2 mM DTT, 0.2 mM Na 2 S 2 O 4 , and 0.5 mM sodium oxalate. The buffer was added to the column through a continuous closed system of tubing, and the bottle containing the buffer was closed by a rubber stopper and pressurized with N 2 gas during use to keep O 2 from entering the buffer and column. Proteins were anaerobically dissolved in the same buffer, removed from the anaerobic chamber in stoppered serum vials, and added to the column sample loop with a Hamilton gas-tight syringe. Blue dextran; a standard protein mixture consisting of carbonic anhydrase (29 kDa), bovine serum albumin (66 kDa), alcohol dehydrogenase (150 kDa), ␤-amylase (200 kDa), apoferritin (443 kDa), and thyroglobulin (669 kDa); OOR; and M. thermoacetica pyruvate ferredoxin oxidoreductase were run separately over the column. Blue dextran and standard proteins were purchased from Sigma-Aldrich.
Sedimentation Equilibrium-The oligomeric state of OOR was determined at 20°C by sedimentation equilibrium under anaerobic conditions using an Optima XL-I analytical ultracentrifuge (Beckman Coulter, Inc.) equipped with an eighthole An50 Ti rotor. OOR was dialyzed against 50 mM Trissulfate buffer (pH 8.0) containing 2 mM DTT and 100 mM NaCl before centrifugation. Sample and reference cells were incubated overnight in an anaerobic glove box (nitrogen atmosphere; Belle Technology) prior to loading with reference buffer and OOR samples. Three concentrations (8.3, 21, and 32 M) of OOR were loaded (110 l) in the sample cells, and the reference cells were filled with the dialysate buffer (125 l). Radial scans were collected at 430, 460, and 470 nm after 22 and 24 h using a rotor speed of 6000 rpm. The scans are an average of 10 measurements at each radial position with a spacing of 0.001 cm. Data were fit by global analysis to an equation describing a single-species model using Origin 6.0. The partial specific volume of OOR used for best fit analysis was 0.7396, which is the average of the three OOR polypeptides calculated by SedTerp. The solvent density of the buffer was calculated to be 1.0038 g/ml.
Miscellaneous Methods-The stoichiometry of the three OOR peptides was estimated from a Coomassie-stained SDSpolyacrylamide gel of the purified protein. To find the relative amounts of the three bands, the gel was scanned and digitized using UN-SCAN-IT gel Version 6.1 software from Silk Scientific (Orem, UT). Blue native polyacrylamide gel electrophoresis was done with the Invitrogen NativePAGE Novex BisTris gel system, using 4 -16% acrylamide gradient gels, and NativeMark protein standards. OOR and PFOR proteins were mixed with 0.5% Coomassie G-250 before loading gels. Protein concentrations were determined by the rose bengal method (19), using a lysozyme standard. The concentration of TPP bound to OOR was determined by a fluorescent thiochrome assay (20). Pure TPP was used as a standard. Metal concentrations were determined by ICP-OES at the Chemical Analysis Laboratory at the University of Georgia. For metal analysis, 1.1 ml of 20 M OOR was dialyzed against two changes of 500 ml of 100 mM Tris-HCl, 2 mM DTT, pH 7.9. Metal concentrations in the protein sample were calculated after subtracting the concentrations in a sample of the dialysis buffer treated exactly as was the protein. (14) showed by SDS-PAGE that 33-and 42-kDa proteins are induced during growth of M. thermoacetica on oxalate. Cell extracts from cells grown with different substrates, SDS-PAGE (supplemental Fig. S1, lanes 2 and 3), and two-dimensional electrophoresis (supplemental Fig. S2) experiments reveal three proteins (with molecular masses of 42, 39, and 35 kDa) that are more strongly expressed in cells grown in stoppered culture flasks on oxalate or on oxalate and glucose than those grown on glucose (in the absence of oxalate). These three proteins were identified by mass spectrometry as the subunits of an annotated 2-oxoacid:ferredoxin oxidoreductase, with NCBI accession numbers of YP_430440, YP_430441, and YP_430442 and Joint Genome Institute locus tag classifications Moth_1593, Moth_1592, and Moth_1591, respectively (21) (supplemental Table S1). Directly upstream from Moth_1593 are two genes that are transcribed in the same direction, which encode a transcriptional regulator (Moth_1595) and an AAA family ATPase in the CDC48 subfamily (Moth_1594). The N-terminal domain of the predicted transcriptional regulator is annotated as a GntR family helix-turn-helix pfam domain, and the C-terminal domain is annotated as an FCD ligand-binding domain. Moth_1590, downstream from Moth_1591, encodes a major facilitator superfamily protein with 35% sequence identity to the oxalate-formate antiporter of O. formigenes (22) (supplemental Fig. S3).

Identification of Proteins Induced during Growth of M. thermoacetica on Oxalate-Daniel and Drake
Purification of Oxalate-degrading Activity from M. thermoacetica-As shown earlier (15), we found that cell extracts containing soluble protein from M. thermoacetica grown on oxalate and glucose catalyzed oxalate-dependent reduction of methyl viologen. Based on an HPLC-based assay that measures the decrease in oxalate concentration, the OOR specific activity was 0.4 mol min Ϫ1 mg protein Ϫ1 at 55°C, whereas that of extracts from cells grown on glucose without oxalate was less than 0.03 mol min Ϫ1 mg protein Ϫ1 at 55°C. We also used the HPLC-based assay to measure oxalate degradation by whole cells of M. thermoacetica grown on oxalate and glucose or on glucose alone. In these assays, which were followed for several h, oxalate was degraded 30 times more quickly by cells that had been previously exposed to oxalate than by cells grown on glucose without oxalate.
To determine if the 2-oxoacid:ferredoxin oxidoreductase homolog that is overexpressed during growth on oxalate is the only protein needed for the oxalate-dependent methyl viologen reducing activity seen in cell extracts, we purified OOR from M. thermoacetica cells to greater than 95% purity, as shown by SDS-PAGE ( Fig. 1 and Table 1). After 22-fold purification, the active protein consisted of three peptides in 1:0.8:1 stoichiometry, with estimated sizes of 36, 43, and 32 kDa, which corresponds well to the predicted masses of 34.2, 43.7, and 33.9 kDa for YP_430440, YP_430441, and YP_430442. The purified OOR preparation retained 0.015 unit/mg of CODH activity, indicating that there is a 0.03% contamination of OOR with CODH/ACS, which apparently was not completely separated from the OOR during the purification procedure.
When run on blue native polyacrylamide gel electrophoresis, most of the protein was found in a major band with an estimated molecular mass of 243 kDa and another species with a mass of 445 kDa (supplemental Fig. S1). Molecular exclusion chromatographic analysis of OOR reveals predominantly a 236-kDa species and a 117-kDa species, which accounts for 10 -20% of the total protein (supplemental Fig. S4). In a parallel gel filtration experiment, the M. thermoacetica PFOR was shown to elute with a similar profile. The quaternary structure of OOR was also characterized by analytical ultracentrifugation. Fig. 2 shows the results from sedimenta-tion equilibrium analysis of OOR at three different concentrations. A best fit value of 226,890 Da was estimated for the molecular mass of OOR which is within 1.5% of the molecular mass predicted for a dimeric species (223,700 Da). These combined results indicate that, like PFOR and other OFORs, OOR forms a dimeric structure consisting of two heterotrimeric units.
Sequence Analysis of OOR-2-oxoacid:ferredoxin oxidoreductases are made up of a minimum of three conserved domains, ␣, ␤, and ␥, with an additional ␦ domain in most OFORs. The genomic arrangement and fusion of these domains varies. The residues involved in binding TPP and one [Fe 4 S 4 ] 2ϩ/1ϩ cluster are in the ␤ subunit (23). Enzymes that include the ␦ subunit have two additional [Fe 4 S 4 ] 2ϩ/1ϩ clusters that are involved in electron transfer from the TPP active site to ferredoxin (24).
The OOR protein is made up of separate ␣ and ␤ subunits and a third subunit that is a fusion of ␥ and ␦ domains (Fig. 3).
We performed separate ClustalW alignments of the sequences of the three OOR subunits with those of corresponding subunits of biochemically characterized pyruvate, 2-oxoisovalerate, 2-oxoglutarate, indole pyruvate, and broad specificity oxidoreductases (see legend to supplemental Fig.  S4). The chosen sequences included the Desulfovibrio africanus PFOR, which has been crystallized in complex with pyruvate (25), allowing us to determine whether or not oxoacid binding residues are conserved in OOR. In the ␤ subunit, residues that coordinate TPP, the adjacent Mg 2ϩ ion of the [Fe 4 S 4 ] cluster that is nearest TPP, are conserved. The eight cysteine residues that bind the other two [Fe 4 S 4 ] clusters are conserved in the OOR ␦ subunit.
In the ␣ and ␤ subunits, two of five substrate-binding residues from D. africanus PFOR are conserved in OOR. Arg 109 corresponds to Arg 114 in D. africanus PFOR, which binds the carboxyl group of pyruvate. Three other residues, Ile 123 , Ile 843 , and Asn 996 , are less strongly conserved among the other OFORS. In the D. africanus PFOR structure, Asn 996 forms a hydrogen bond to the carbonyl group, and Ile 843 and Ile 123 interact with the methyl group of pyruvate. The corresponding residues are Asn 143 and Val 55 in the ␤ subunit and Phe 117 in the ␣ subunit of OOR. The fifth residue, Thr 31 in PFOR, is part of a strongly conserved YPITP motif that is present in OFORs using many different substrates (26) and is within hydrogen bonding distance of the ␣-keto oxygen of pyruvate in the D. africanus PFOR crystal structure. This Thr residue is replaced by Arg in the OOR sequence (supplemental Fig. S5), thus forming a YPIRP motif. A BLAST search of the non-redundant protein data base at NCBI shows that this substitiution is rare; eight of the 11 sequences most closely related to OOR have Thr 31 replaced with arginine, but no other sequence in the first 500 BLAST hits has arginine in this position.
Cofactor Binding-OOR was purified in buffer containing 1 mM TPP and 2 mM MgCl 2 , and, during the purification, the   Oxalate Oxidoreductase DECEMBER 24, 2010 • VOLUME 285 • NUMBER 52 protein was assayed in the same buffer. After purification, the protein was exchanged into 50 mM Tris-HCl with 2 mM DTT but without TPP or MgCl 2 . The activity of the protein did not change after buffer exchange, and we found that it was not necessary to add TPP to assays to see full activity. Using a fluorescent assay to quantify thiochrome bound to OOR, 1.0 mol of TPP were found per mol of protein.
As-isolated OOR is brown; metal analysis by ICP showed 14 mol of iron and 0.8 mol of Mg 2ϩ per mol of protein. After preparing the protein for metal analysis by dialysis into metalfree buffer, the protein retained activity with 0.7 mol of TPP/ mol of protein. Mg 2ϩ is likely to help with TPP binding as it does in other TPP-dependent enzymes. Calcium, sodium, and potassium were also present in the sample in greater than stoichiometric amounts, but their concentrations varied widely between two metal analysis samples, so it is possible that they bind nonspecifically to the protein ( Table 2).
Catalytic Properties of OOR-The purified heterotrimeric protein catalyzed the oxidation of oxalate using methyl viologen as an electron acceptor with a specific activity of 0.05 mol min Ϫ1 mg Ϫ1 (k cat ϭ 0.095 s Ϫ1 at pH 7.9) and a K m for oxalate of 58 Ϯ 6 M (Fig. 4A). The addition of CoA from 1 M to 1 mM had no effect on the OOR reaction rate (Fig. 4A). In addition, the addition of 1 mM ATP and 1 mM CoA together to the assay mixture did not affect the rate of the reaction. The pH dependence of OOR activity was measured at pH values between 5.0 and 10.2 using saturating concentrations of oxalate (1 mM). The maximum activity (0.11 unit/mg, 0.21 s Ϫ1 ) was observed at pH 8.7 (Fig. 4B). Other reported optimal pH values for activity of 2-oxoacid:Fd oxidoreductases are between pH 7.5 and 9.0 (27)(28)(29)(30)(31)(32)(33). Because the pK a values for oxalate are 1. 23 and 4.19 (34), the fully deprotonated form would be the substrate at the optimal pH for OOR.
OOR showed slow activity with all 2-oxoacids tested ( Table  3). The OOR preparation had no formate dehydrogenase activity, which indicates that the decarboxylase activity of OOR, like other OFORs, is strictly coupled to electron carrier reduc-tion, unlike the decarboxylases, where the electron pair is retained in the other product (e.g. pyruvate decarboxylase generates CO 2 and acetaldehyde). These results are consistent with earlier assays of cell lysates (15), in which it was shown that the oxalate-catabolizing system in M. thermoacetica had different electron acceptor specificity than formate dehydrogenase. OOR was active with a broad range of electron acceptors (Table 4). OOR used CODH as an electron acceptor, catalyzing the oxalate-dependent reduction of CO 2 to CO.
Spectral Characterization of OOR-The UV-visible spectrum of as-isolated OOR had a broad absorbance shoulder between 300 and 500 nm (Fig. 5). The absorbance in this re-   gion increased when the protein was exposed to air or treated with ferricyanide. Treating the protein with sodium dithionite decreased the absorbance of the band between 300 and 500 nm, which is consistent with the bleaching seen upon reduction of [Fe 4 S 4 ] 2ϩ/1ϩ clusters in other proteins (e.g. PFOR) (24). OOR is expected to bind three [Fe 4 S 4 ] clusters, based on the sequence alignments with PFOR and on the metal analyses described above. The maximum difference in absorbance between the oxidized and reduced protein was at 420 nm with a difference extinction coefficient (⌬⑀) of 24.9 mM Ϫ1 cm Ϫ1 , or 8.3 per mM [Fe 4 S 4 ], which is similar to the extinction coefficients of 7-7.5 per mM cluster that have been reported for PFOR (24,35). When OOR was incubated with oxalate, an identical difference spectrum was observed, and after oxalate reduction, OOR was oxidized by the addition of a catalytic amount of CODH/ACS and excess CO 2 . Because [Fe 4 S 4 ] clusters are diamagnetic in the oxidized (2ϩ) state and paramagnetic in the reduced (1ϩ) state, OOR was studied by EPR to follow reduction of the protein by oxalate. The UV-visible spectrum of the protein used in this experiment was used to calculate that the as-isolated protein had 1.7 clusters reduced. As-isolated OOR exhibited an EPR spectrum (Fig. 6) with g values of 2.039, 1.955, 1.927, 1.887, and 1.865 (1.2 spins/mol). When a stoichiometric amount of oxalate was added to the protein, the signal intensity increased to 3.4 spins/mol. The 1.955 and 1.865 features became broader, and an additional feature at g ϭ 2.007 was seen in samples of fully reduced OOR prepared with oxalate or FIGURE 5. UV-visible spectra of OOR. Solid line, as-isolated protein; dashes and dots, oxidized protein; short dashes, oxalate-reduced protein; dots, dithionite-reduced protein. Spectra were measured anaerobically. 4.1 M protein was prepared in 50 mM sodium phosphate, pH 7.0. Reduced protein was prepared by adding 10 M sodium oxalate or 15 M sodium dithionite to the as-isolated protein sample, and the spectra shown were recorded after 20 min. Oxidized protein was prepared by incubation in 6.8 mM CO 2 with 80 nM CODH/ACS from M. thermoacetica.    Oxalate Oxidoreductase DECEMBER 24, 2010 • VOLUME 285 • NUMBER 52 JOURNAL OF BIOLOGICAL CHEMISTRY 40521 dithionite (3.2 spins/mol). This EPR spectrum was saturated above 0.129 milliwatt at 9 K. The relative intensities of the 2.039 and 1.927 features decreased as the power was increased, although they were still present at 129 milliwatts. When oxalate-reduced OOR was oxidized by the additions of oxidized cytochrome c, there was no change in the overall shape of the EPR spectrum, even in the almost completely oxidized protein (0.07 spins/mol, Fig. 6F), indicating that the potentials of the iron-sulfur clusters are similar. The g values of the OOR spectra are typical of [Fe 4 S 4 ] 2ϩ/1ϩ clusters, such as those in eight iron (2-[Fe 4 S 4 ]) ferredoxins (36,37) and other OFOR enzymes (35,38). As eight iron ferredoxins go from partially to fully reduced, an increase in complexity of the EPR spectrum is seen, which results from spin-spin interactions between two reduced [Fe 4 S 4 ] clusters. Similar coupling of clusters has been seen in PFOR (35), as well as spin-spin interaction between an [Fe 4 S 4 ] cluster and a substrate-derived radical in the active site (38). We did not see an increase in complexity with increasing reduction of OOR, nor did we see a substrate-derived radical when OOR was frozen ϳ30 s after mixing with oxalate.

DISCUSSION
Oxalate is an important metabolite that is produced in the soil and in the animal gastrointestinal tract, with elevated levels causing kidney stones. Many bacteria that use oxalate as an energy source also use oxalate as a carbon source by reduction to glyoxylate, which is incorporated into the central metabolite 3-phosphoglycerate (39,40), but enzymes involved in glyoxylate incorporation have not been detected in oxalategrown cultures of M. thermoacetica (3,13). We have shown that M. thermoacetica metabolizes oxalate very simply by a novel CoA-independent OFOR that catalyzes the oxidative decarboxylation of oxalate to 2 mol of CO 2 , coupled to the reduction of ferredoxin (or other electron acceptors). The apparent reaction is C 2 O 4 2Ϫ ϩ Fd ox 7 2 CO 2 ϩ Fd red i , 3 although we have not yet shown whether CO 2 or bicarbonate is the product that is released from OOR. Our results are consistent with previous studies, which indicate that incorporation of oxalate into cell material requires conversion to CO 2 (3,14). For example, M. thermoacetica can grow on oxalate even in CO 2 -free medium; however, when cells are grown on oxalate and CO 2 , very little radioactivity from 14 C-oxalate is incorporated into biomass (14). Thus, OOR enables growth on oxalate by linking the production of CO 2 and reducing equivalents to the Wood-Ljungdahl pathway of autotrophic anaerobic acetyl-CoA formation (3,14).
Oxalate induces the expression of three proteins (36,43,and 32 kDa) in M. thermoacetica that can be resolved by twodimensional PAGE. Earlier studies described the induction of two protein bands (one of the bands apparently was not resolved by one-dimensional SDS-PAGE) when M. thermoacetica was grown on oxalate, relative to growth on CO, formate, or glyoxylate (14). Genome-enabled mass spectrometric results reveal that the oxalate-induced proteins belong to the OFOR family and are encoded within an operon that includes a homolog of O. formigenes oxalate-formate antiporter, a permease and a gene encoding a transcriptional regulatory protein, for which we propose the name OorR. OorR contains an N-terminal helix-turn-helix DNA binding domain and a Cterminal ligand-binding domain and is likely to regulate expression of OOR and coordinate its expression with expression of the enzymes of the Wood-Ljungdahl pathway. Oxalate is a unique substrate for M. thermoacetica, which uses nitrate instead of CO 2 as an electron acceptor when grown on other multiple-carbon growth substrates in the presence of nitrate and CO 2 . However, CO 2 is used preferentially over nitrate when it is growing on oxalate (3). Nitrate appears to inhibit autotrophic growth by repressing the Wood-Ljungdahl pathway at the transcriptional level (1,2). We suggest that interactions between oxalate, OorR, and the promoters of the oor and acs operons are able to induce the oor operon and prevent the nitrate-dependent repression of the Wood-Ljungdahl pathway. M. thermoacetica does not produce formate from oxalate, but acetate, the end product of the Wood-Ljungdahl pathway, could be exchanged with oxalate by the antiporter.
OOR is the first protein shown to catalyze anaerobic oxalate oxidation, unlike previously described anaerobic enzymes that produce CO 2 and formyl-CoA (6,9). Although several features (e.g. Fe 4 S 4 clusters and TPP content) of OOR are similar to other members of the OFOR family, OOR is unique among family members in its lack of requirement for CoA. Fig. 7 shows a proposed mechanism of OOR, based on the results described here and on analogy with conserved features of other OFORs, such as PFOR, whose mechanism has been extensively studied (24,35,42) and whose crystal structure is known (25).
Steps 1 and 2: Binding of TPP, Mg 2ϩ , and Oxalate-The early steps of the reaction would resemble those of all TPPdependent enzymes, reviewed recently (43). After binding oxalate (step 1), step 2 of the proposed OOR mechanism in-FIGURE 7. Proposed OOR mechanism. Oxalate binds to OOR (step 1) and undergoes nucleophilic attack by the TPP ylide (step 2), generating oxalyl-TPP, which could be stabilized by protonation from a general base (step 3). Decarboxylation (step 4) leaves an anionic intermediate that would release two electrons to the [Fe 4 S 4 ] clusters (step 5). The carboxyl-TPP that remains could be decarboxylated to release a second CO 2 molecule (step 6) or hydrolyzed to release bicarbonate (not shown), regenerating the starting form of the enzyme.
volves nucleophilic attack of the anionic ylide of OOR-bound TPP on oxalate to generate an oxalyl-TPP adduct. The purified OOR contains Mg 2ϩ and TPP, which were added during purification because in some OFORs, like PFOR from M. thermoacetica, TPP dissociates rather easily, resulting in loss of activity in buffer lacking TPP. However, TPP remains tightly bound to OOR even after extensive buffer exchange and over several months' storage. Based on sequence homology between OOR and the D. africanus PFOR, whose structure is known (25), we find that most of the key residues in PFOR that are involved in binding and activating TPP to generate the deprotonated ylide are conserved in OOR. These include ␤ subunit residues Cys 130 and Asn 143 (Cys 840 and Asn 996 in D. africanus PFOR), which interact with the pyrophosphate moiety of TPP. Conserved residues that interact with the Mg 2ϩ ion include a G 109 DGX 32 N motif (G 962 DGX 32 N in PFOR). Another key conserved residue is Asp 59 (Asp 64 in PFOR), which interacts with the N1Ј pyrimidine group of TPP and plays a key role in deprotonation of the C-2 of the thiazole ring to generate the active ylide. Conserved residue Asn 996 in PFOR (Asn 143 ) forms a hydrogen bonding interaction with the thiazolium sulfur of TPP, and a large hydrophobic residue, Phe 869 of PFOR (Ile 159 in the ␤ subunit of OOR), interacts with TPP to promote formation of a V-conformation between the pyrimidine and thiazolium ring, which is conserved in all TPP-dependent enzymes so far studied. Different residues can stabilize the V-conformation in TPP-dependent enzymes; Ile is also found in this position in pyruvate decarboxylase (44), Met and Leu are found in pyruvate oxidase and transketolase, and other OFOR enzymes have His and Tyr.
Of the 2-oxoacid substrates we tested, oxalate was oxidized most quickly. Furthermore, the low K m for oxalate (ϳ60 M) and the fact that the enzyme is induced in the presence of oxalate are consistent with oxalate being the physiological substrate for the enzyme. Although OOR can oxidize glyoxylate, the enzyme is not induced in cells grown on glyoxylate (14). Thus, we think it unlikely that OOR would be involved in glyoxylate metabolism in M. thermoacetica. OOR contains conserved residues that interact with the carboxyl and carbonyl groups of pyruvate in PFOR but contains substitutions that we propose are important for binding the carboxyl group of oxalate that replaces the acetyl group of pyruvate.
Several residues involved in pyruvate binding in PFOR are conserved in OOR, including Arg 109 (Arg 114 in PFOR, which binds the carboxyl group of pyruvate) and Asn 143 (Asn 996 in PFOR forms hydrogen bonds to the carbonyl group of pyruvate). Substitution of the Arg in Sulfolobus tokodaii that corresponds to Arg 114 abolished activity (45). As expected, Ile 123 and Ile 843 , which interact with the methyl group of PFOR, are not conserved in OOR. Thr 31 in PFOR is a highly conserved residue among the OFORs and is part of a YPITP motif that is important for catalysis. Substitutions of the end residues of this motif in an Aeropyrum pernix OFOR destroy activity, and separate mutations of the three middle residues have varying effects that are in general larger for k cat than for K m , which suggests that the motif is important during reaction turnover (26). However, Thr 31 is replaced with Arg 31 in the OOR. In-clusion of a second Arg in the active site may help to stabilize the additional negative charge on oxalate. Because Arg in this position is found in only those few sequences in the NCBI protein data base that have high identity to that of OOR, we propose that a YPIRP motif replaces the usual conserved YPITP motif to promote interactions that may be characteristic of OORs.
Decarboxylation and Electron Transfer-In analogy to the mechanism of PFOR (24), a general base would be expected to stabilize the negative charge on the oxalyl-TPP intermediate in step 3, followed by decarboxylation of the oxalyl-TPP adduct in step 4 to yield a reactive anionic carboxy-TPP intermediate that, in step 5, would release two electrons into the internal electron transfer pathway consisting of three [Fe 4 S 4 ] 2ϩ/1ϩ clusters. OOR contains sufficient iron to accommodate these three clusters. In addition, all 12 Cys residues that bind the three clusters of PFOR are conserved in the sequence of OOR. Furthermore, EPR and UV-visible experiments clearly showed that OOR contains three [Fe 4 S 4 ] 2ϩ/1ϩ and that oxalate can reduce all of these clusters. Because oxalate is a two-electron donor, full reduction of all of the clusters would require 2 mol of oxalate.
Because [Fe 4 S 4 ] clusters accept one electron at a time, there is likely to be a transient intermediate in which one of the clusters is reduced by the anionic carboxy-TPP anion to generate a carboxy-TPP radical (not shown); transient kinetic experiments are under way to test this hypothesis.
In this step of the PFOR reaction, binding of CoA accelerates the electron transfer reaction by 10 5 -fold. However, OOR is a unique member of this family in having no requirement for CoA; therefore, step 6 would involve a second decarboxylation to regenerate the active ylide for the next round of catalysis. Although it seems less likely, it is possible that this carboxy-TPP adduct undergoes hydrolysis to release bicarbonate instead of CO 2 . One of the proposed roles of CoA in PFOR and other OFORs is to generate a highly reducing anionic intermediate that could transfer electrons to the clusters (24,46), and the same role has been proposed for phosphate in Lactobacillus plantarum pyruvate oxidase, which forms acetyl-phosphate and CO 2 from pyruvate (47). Perhaps the negative charge on the OOR carboxy-TPP adduct is sufficiently reducing to make CoA unnecessary.
In the final steps of the reaction, the two-electron-reduced state of OOR transfers its reducing equivalents from the internal electron transfer wire of [Fe 4 S 4 ] clusters to an external electron acceptor. Like PFOR, OOR can use a wide range of acceptors and is unable to transfer electrons to pyridine nucleotides. Ferredoxin is one of these and is a likely physiological electron acceptor that could carry electrons to enzymes of the Wood-Ljungdahl pathway. The rates were similar among all electron acceptors that were found to work. This suggests that the rate of catalysis is limited by some step other than electron transfer from the enzyme to the acceptor. Interestingly, like PFOR, OOR can transfer electrons directly to CODH to generate CO. This could be a physiologically relevant reaction in vivo because electrons from oxalate are used in the synthesis of acetyl-CoA (3) and because M. thermoacetica can be cultured on oxalate even in CO 2 -free medium (14).