Building Protein-Protein Interaction Networks with Proteomics and Informatics Tools*
- From the ‡Stowers Institute for Medical Research, Kansas City, Missouri 64110 and
- the §Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas 66160
- ↵1 To whom correspondence should be addressed. E-mail: mpw{at}stowers.org.
Abstract
The systematic characterization of the whole interactomes of different model organisms has revealed that the eukaryotic proteome is highly interconnected. Therefore, biological research is progressively shifting away from classical approaches that focus only on a few proteins toward whole protein interaction networks to describe the relationship of proteins in biological processes. In this minireview, we survey the most common methods for the systematic identification of protein interactions and exemplify different strategies for the generation of protein interaction networks. In particular, we will focus on the recent development of protein interaction networks derived from quantitative proteomics data sets.
- Computation
- Mass Spectrometry (MS)
- Protein-Protein Interactions
- Proteomics
- Yeast
- Networks
- Quantitative
Footnotes
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↵* This work was supported by the Stowers Institute for Medical Research. This is the fourth article in the Thematic Minireview Series on Computational Systems Biology. This minireview will be reprinted in the 2011 Minireview Compendium, which will be available in January, 2012
- © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.
