Distinct Functions of the Mitogen-activated Protein Kinase-activated Protein (MAPKAP) Kinases MK2 and MK3

In LPS-treated macrophages, activation of STAT3 is considered to be crucial for terminating the production of inflammatory cytokines. By analyzing the role of MAPK-activated protein kinase (MK) 2 and MK3 for LPS-induced STAT3 activation in macrophages, the present study provides evidence that MK2 is crucial for STAT3 activation in response to LPS because it prevents MK3 from impeding IFNβ gene expression. Accordingly, LPS-induced IFNβ gene expression is down-regulated in MK2-deficient macrophages and can be reconstituted by additional ablation of the MK3 gene in MK2/3−/− macrophages. This is in contrast to LPS-induced IL-10 expression, which essentially requires the presence of MK2. Further analysis of downstream signaling events involved in the transcriptional regulation of IFNβ gene expression suggests that, in the absence of MK2, MK3 impairs interferon regulatory factor 3 protein expression and activation and inhibits nuclear translocation of p65. This inhibition of p65 nuclear translocation coincides with enhanced expression and delayed degradation of IκBβ, whereas expression of IκBα mRNA and protein is impaired in the absence of MK2. The observation that siRNA directed against IκBβ is able to reconstitute IκBα expression in MK2−/− macrophages suggests that enhanced expression and delayed degradation of IκBβ and impaired NFκB-dependent IκBα expression are functionally linked. In summary, evidence is provided that MK2 regulates LPS-induced IFNβ expression and downstream STAT3 activation as it restrains MK3 from mediating negative regulatory effects on NFκB- and interferon regulatory factor 3-dependent LPS signaling.

In LPS-treated macrophages, activation of STAT3 is considered to be crucial for terminating the production of inflammatory cytokines. By analyzing the role of MAPK-activated protein kinase (MK) 2 and MK3 for LPS-induced STAT3 activation in macrophages, the present study provides evidence that MK2 is crucial for STAT3 activation in response to LPS because it prevents MK3 from impeding IFN␤ gene expression. Accordingly, LPS-induced IFN␤ gene expression is down-regulated in MK2deficient macrophages and can be reconstituted by additional ablation of the MK3 gene in MK2/3 ؊/؊ macrophages. This is in contrast to LPS-induced IL-10 expression, which essentially requires the presence of MK2. Further analysis of downstream signaling events involved in the transcriptional regulation of IFN␤ gene expression suggests that, in the absence of MK2, MK3 impairs interferon regulatory factor 3 protein expression and activation and inhibits nuclear translocation of p65. This inhibition of p65 nuclear translocation coincides with enhanced expression and delayed degradation of IB␤, whereas expression of IB␣ mRNA and protein is impaired in the absence of MK2. The observation that siRNA directed against IB␤ is able to reconstitute IB␣ expression in MK2 ؊/؊ macrophages suggests that enhanced expression and delayed degradation of IB␤ and impaired NFB-dependent IB␣ expression are functionally linked. In summary, evidence is provided that MK2 regulates LPS-induced IFN␤ expression and downstream STAT3 activation as it restrains MK3 from mediating negative regulatory effects on NFB-and interferon regulatory factor 3-dependent LPS signaling.
Recognition of pathogen invasion by macrophages is mediated via activation of receptor complexes specialized to detect pathogen-derived molecular patterns. These receptors repre-sent a front line recognition system required to initiate innate immunity.
In macrophages, LPS induces an inflammatory response via binding to the TLR4-MD2 receptor complex (1). Subsequently, the activated TLR4 receptor complex elicits its biological actions via activation of an intracellular signaling network that involves activation of the IKK/IB␣/NFB cascade, of IRF3 2 or of members of the MAPK family such as p38 MAPK (2). These different pathways are interlinked in the control of the expression of inflammatory gene products at different levels including the level of transcription, mRNA stability, translation, posttranslational modification, and protein stability. In macrophages, the activation of STAT3 is crucial for terminating the production of inflammatory cytokines (3,4), in particular because of its role as the key mediator that transmits the antiinflammatory effects of IL-10 (5, 6).
Type I interferons (IFN␣/␤) have been identified as crucial mediators of the LPS-induced production of IL-10 (7), indicating that they are crucial for launching anti-inflammatory effects on macrophages. Consistently, IFN␤-deficient macrophages show an enhanced inflammatory response toward LPS (8).
p38 MAPK -mediated activation of MK2 and MK3 is essential for the production of several LPS-induced inflammatory cytokines such as TNF␣ (9,10). In addition, upon activation by LPS or TNF␣, the p38 MAPK /MK2 cascade also suppresses STAT3 activation by cytokines such as IL-6 (11) via induction of SOCS3 (11)(12)(13), warranting that sustained STAT3 activation occurs only in the presence of IL-10, which, in contrast to IL-6, is insensitive toward the inhibitory effects of LPS (14). Congruently, in SOCS3-deficient macrophages IL-6 indeed mediates IL-10-like anti-inflammatory effects (15). This suggests that in macrophages the p38 MAPK /MK2/3 pathway holds a key position in propagating the inflammatory response, because it mediates inflammatory cytokine expression and prevents cytokines such as IL-6 from untimely activating anti-inflammatory signals such as sustained activation of STAT3.
The present study analyzes the role of MK2 and MK3 for LPS-induced STAT3 activation in macrophages revealing unexpected and novel details on the function of MK3 for the regulation of IFN␤ and IB␣ expression. The present manuscript for the first time demonstrates that MK2 and MK3, which so far appeared as closely related, cooperatively acting isoenzymes (10,16), also have different regulatory roles, with MK2 regulating gene expression by preventing MK3-mediated negative regulatory effects on signaling.
Immortalized Macrophage Cell Lines-Immortalized murine macrophages were established from bone marrow-derived macrophages (BMDM) that were isolated from mice deficient for MK2 (MK2 Ϫ/Ϫ M⌽), for both MK2 and MK3 (MK2/3 Ϫ/Ϫ M⌽), or from wild type animals (WT M⌽) by retroviral infection with a virus encoding both the v-Raf and the v-Myc oncogene as described recently (16). The resulting immortalized macrophage populations were 91% (Ϯ 2.7) positive for the macrophage-specific marker F4/80 and for CD11b as assessed by FACS analysis (data not shown) without significant differences between macrophages derived from wild type animals and those derived from MK2 Ϫ/Ϫ or MK2/3 Ϫ/Ϫ animals. The cells were cultivated in DMEM including 1000 mg/liter glucose with glutamax supplemented with 10% heat-inactivated FCS and incubated in a thermoincubator (37°C and 5% CO 2 ), and medium was changed every day. For the experiments, the cells were seeded on 60-or 100-mm л dishes with a final confluence of ϳ50%.
Preparation and Cultivation of Primary Murine Bone Marrow-derived Macrophages-Mice deficient for MK2, MK3, MK2/MK3, or IFNAR; their genotyping; and maintenance of the colony have been described elsewhere (9, 16 -18). To generate WT BMDM, IFNAR-deficient (IFNAR Ϫ/Ϫ BMDM), MK2-deficient (MK2 Ϫ/Ϫ BMDM), MK3-deficient (MK3 Ϫ/Ϫ BMDM), and BMDM deficient for both MK2 and MK3 (MK2/ 3 Ϫ/Ϫ BMDM), bone marrow cells (ϳ2 to 3 ϫ 10 7 ) were flushed from the femurs of mice. The cells were cultured in 75-cm 2 culture flasks in DMEM including 1000 mg/liter glucose supplemented with 10% heat-inactivated fetal calf serum, glutamax, 100 units/ml penicillin G, 100 mg/ml streptomycin, and 10 ng/ml recombinant murine M-CSF. The cells were incubated in an incubator at humidified atmosphere (37°C, 5% CO 2 ). Culture medium (10 ml/culture flask) was replaced after 3 days to remove nonadherent cells and cell debris, and at day 6, 30% of the initial volume was added as fresh medium containing 10 ng/ml M-CSF. To achieve a homogenous distribution of the macrophages on the culture dish, the cells were detached after 7 days of culture using trypsin/EDTA solution (incubation for 30 min at 37°C). Thereafter, the cells were seeded on 60-mm л dishes at a density of ϳ1 ϫ 10 6 cells/dish, and culture was continued for another 2 days. In the end, this procedure resulted in a yield of ϳ7-9 ϫ 10 6 cells at maximum that were to 99% (Ϯ0.7) F4/80-positive, CD11b-positive, and CD11c-negative macrophages as assessed by FACS analysis (data not shown). The medium was changed to M-CSF-free culture medium 16 h before experiments were performed. The cells were used after a total differentiation period of 9 days.
Preparation and Cultivation of Human Peripheral Blood-derived Macrophages-Human peripheral blood mononuclear cells (PBMCs) were isolated from freshly collected leukocyterich buffy coats obtained from healthy blood donors (University of Düsseldorf, Blood Transfusion Service) by a density gradient centrifugation over Ficoll-Paque gradient (GE Healthcare) and 12 ϫ 10 6 cells/dish (100-mm л dish) were seeded. Monocytes were allowed to adhere to the plastic bottom of the dish for 2 h at 37°C in RPMI 1640 medium supplemented with antibiotics and 10% fetal calf serum. After this incubation period, the nonadherent cells were removed, and the wells were washed with culture medium. The cells were incubated in a thermoincubator (37°C and 5% CO 2 ). Monocytes were differentiated into macrophages in RPMI 1640 medium supplemented with antibiotics, 10% fetal calf serum, and recombinant human M-CSF (10 ng/ml). Culture medium (6 ml/dish) was replaced after 3 days, and total medium volume was increased by 30% after further 3 days. The cells were used after 9 days of cultivation and differentiation. The medium was changed to M-CSF-free culture medium 16 h before the experiments were performed.
Retroviral Gene Transfer-Retroviral gene transfer into MK2/3 Ϫ/Ϫ M⌽ was performed as outlined recently (16). Briefly, full-length murine MK2 and MK3 were subcloned into the pMMP-IRES-GFP (kind gift of C. Klein, Hannover Medical School, Hannover, Germany) bicistronic retroviral vector upstream of the IRES. To obtain MK2/3 Ϫ/Ϫ macrophage cell lines stably reconstituted with either MK2 or MK3, retroviral supernatants were generated by transient transfection of the BD EcoPack 2-293 packaging cell line with bicistronic vectors encoding the gene of interest and GFP as marker and were used for infection. The cells were cultivated as described above.
Transfection of siRNA-For targeted gene knockdown, siRNA specific for murine IB␤ (NFKBIB) or nontargeting control pool 2 were purchased from Dharmacon Inc. (Lafayette, CO). 25 nM siRNA were transfected using DharmaFECT 4 with 2 l/ml volume according to the manufacturer's instructions. After an incubation period of 16 h at 37°C in a thermoincubator, the cells were washed and incubated for 48 h in total.
Immunoprecipitation-Cytosolic fractions of macrophages were prepared as described above and were incubated with a polyclonal antibody specific for IB␤ or with an IgG control antibody as well as with a biotinylated anti-rabbit antibody for 120 min at 4°C and subsequently incubated with Dynabeads M-280 streptavidin (Invitrogen) for an additional 120 min at 4°C. The immune complexes were precipitated with the magnetic particle concentrator Dynal MPC-S (Invitrogen) and washed extensively with wash buffer (0,1% Triton, 20 mM Tris/ HCl, pH 7.4, 136 mM NaCl, 2 mM EDTA, 50 mM ␤-glycerophosphate, 20 mM sodium pyrophosphate, 1 mM Na 3 VO 4 , 4 mM benzamidine, 0.2 mM Pefabloc, 5 g/ml aprotinin, 5 g/ml leupeptin, and 10% glycerol). Finally, the samples were incubated at 95°C for 30 min and separated by SDS-PAGE as described above. After blotting, the blots were analyzed using antibodies specific for p65 and IB␤.
Immunoblotting and Immunodetection-For immunoblot analysis of total protein extracts, the cells were grown in a 60-mm ဧ dish and stimulated with LPS or with the respective cytokine at the concentrations indicated. For the analysis of cytosolic lysates and nuclear extracts, the cells were grown in a 100-mm ဧ dish using two dishes for one sample. The respective amounts of protein indicated in the figure legends were subjected to SDS gel electrophoresis (8 and 10% polyacrylamide). The electrophoretically separated proteins were transferred onto PVDF membranes by the semidry Western blotting method. Nonspecific binding was blocked with 5% BSA in TBS-T (20 mM Tris/HCl, pH 7.4, 137 mM NaCl, and 0.1% Tween) for 60 min. The blots were incubated overnight at 4°C with primary antibodies diluted in TBS-T (1:2000). After extensive washing with TBS-T, the blots were incubated with goat anti-rabbit IgG or rabbit anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase and diluted in TBS-T (1:5000) for 1 h at room temperature. After further rinsing in TBS-T, the immunoblots were developed with the ECL system following the manufacturer's instructions (PerkinElmer Life Sciences).
RNA Isolation and Real Time PCR-Total cellular RNA was isolated by using the RNeasy miniprep kit (Qiagen) as described in the manufacturer's instructions. 1 g of total RNA was reverse transcribed with a Quantitect reverse transcription kit (Qiagen) using oligo(dT), which included DNase I digestion. cDNA was diluted 1:5, and 1.2 l of the diluted cDNA was added as template to a final volume of 25 l including 1ϫ SYBR Green PCR Master Mix according to the manufacturer's instructions (Applied Biosystems). The following primers were used for real time PCR: mouse IB␣ (sense, 5Ј-ACG AGC AAA TGG TGA AGG AG-3Ј; antisense, 5Ј-CCA AGT GCA GGA ACG AGT CT-3Ј), mouse IL-10 (sense, 5Ј-CCA AGC CTT ATC GGA AAT GA-3Ј; antisense, 5Ј-TCT CAC CCA GGG AAT TCA AA-3Ј), mouse IFN␤1 (sense, 5Ј-CCC TAT GGA GAT GAC GGA GA-3Ј; antisense, 5Ј-ACC CAG TGC TGG AGA AAT TG-3Ј), and the Fp subunit of the murine succinate dehydrogenase (SDHA) (sense, 5Ј-TGG GGA GTG CCG TGG TGT CA-3Ј; antisense, 5Ј-GTG CCG TCC CCT GTG CTG GT-3Ј). All of the primers were purchased from Eurofins MWG Operon (Ebersberg, Germany). Specificity of RT-PCR was controlled by no template and no reverse-transcriptase controls. Semiquantitative PCR results were obtained using the ⌬C T method. As control gene SDHA was used. Threshold values were normalized to SDHA. Data from at least three independent experiments are presented as means plus standard error of means (S.E.).
Statistical Evaluation-Statistics were calculated using the Prism 5.0 software from GraphPad. Each of the experiments was repeated at least three times. The significance was calculated using a one-or two-way analysis of variance test together with a Bonferroni test. p Ͻ 0.05 was considered to be significant.

MK2 Is Crucial for LPS-mediated STAT3
Activation-In macrophages, LPS induces an activation of STAT3 (Fig. 1, A  and B), which was delayed compared with the immediate STAT3 activation observed in response to IL-6 ( Fig. 1C). As demonstrated in Fig. 1, in human and mouse macrophages, STAT3 activation becomes detectable 80 -120 min after the addition of LPS to the culture medium at the earliest and lasts for at least 6 -7 h.
Previous data indicated that MK2, which is rapidly activated in response to LPS, is important for induction of SOCS3 by TNF␣ (19) and LPS 3 and thus mediates negative regulatory sig-nals on STAT3-dependent cytokine signaling. We used MK2deficient macrophages to address, whether MK2 is also involved in the control of delayed STAT3 activation after LPS treatment. As demonstrated in Fig. 2 (A and B), LPS-mediated activation of STAT3 was strongly affected in MK2-deficient macrophages but not in macrophages that lack MK3, a member of the MK family that is closely related to MK2 (Fig. 2C). This role of MK2 in mediating STAT3 activation is specific for LPS, because activation of STAT3 in response to cytokines such as IL-6 or IFN␤ (Fig. 2, D and E) is not significantly affected upon ablation of the MK2 gene. At a first glance, these data suggest that MK2 but not MK3 is of key importance for mediating  After the respective time periods, the total protein extracts were prepared, and 80 g of protein was subjected to immunoblot analysis. Protein phosphorylation and/or expression was assessed as indicated using antibodies specifically recognizing STAT3-Tyr 705 , p38-Thr 180 /Tyr 182 , and antibodies that recognize total protein of STAT3, MK2, MK3, p38 MAPK , SOCS3, and GAPDH. delayed and sustained STAT3 activation in response to LPS. Because MK3 has been reported to cooperate with MK2 in regulation of TNF␣ expression (16), LPS-induced STAT3 activation was further assessed in macrophages depleted of both MK2 and MK3 (MK2/3 Ϫ/Ϫ ). Unexpectedly, and in contrast to the single ablation of the MK2 gene, combined ablation of MK2 and MK3 (Fig. 2B) resulted in the recovery of LPS-induced STAT3 activation almost back to wild type levels. This observation suggests a yet unknown interrelationship between MK2 and MK3, in which knock-out of MK2 results in an imbalance of the system permitting otherwise unapparent negative regulatory effects of MK3.
LPS-inducible Expression of IL-10 and IFN␤ Is Differentially Regulated by MK2 and MK3-Recently it has been demonstrated that activation of the IFNAR is crucial for LPS-induced IL-10 expression and subsequent STAT3 activation (7). In line with this, IFNAR-deficient macrophages do not exhibit LPSinduced STAT3 activation (Fig. 3). We next addressed the question whether type I interferon expression is regulated by MK2-MK3 interaction. As demonstrated in Fig. 4A, LPS-in-FIGURE 3. LPS-induced STAT3 activation requires IFNAR. BMDM were prepared from wild type or IFNAR-deficient mice and treated for 120 min with LPS (100 ng/ml). Thereafter, total protein extracts were prepared, and 80 g of protein were subjected to immunoblot analysis using antibodies specifically recognizing STAT3-Tyr 705 and total STAT3. ␤-Actin was detected for loading control.  A and B) and IL-10 mRNA expression (C and D). Semiquantitative PCR results were obtained using the ⌬C T method. For A and C, cytokine mRNA was normalized to SDHA, and relative mRNA levels were expressed as fractions of the normalized value of the control, which was set to 100%, whereas for the decay experiments, relative IFN␤ mRNA and IL-10 mRNA levels are expressed as fractions of the normalized value at 0-min time point actinomycin D, which was set to 100%. The data are presented as the means plus S. E. (n ϭ 3). The statistics were calculated as outlined under "Experimental Procedures," and a p value smaller than 0.05 was considered significant. JULY 8, 2011 • VOLUME 286 • NUMBER 27 duced IFN␤ expression was substantially reduced in MK2 Ϫ/Ϫ macrophages in comparison with wild type macrophages. This down-regulation of LPS-mediated IFN␤ expression again was dependent on the presence of MK3 (Fig. 4A).

Role of MK2 and MK3 for STAT3 Activation
Because the turnover of the IFN␤ transcript is strictly regulated (20,21) and MK2 is known to be important for regulation of transcript stability, the influence of MK2 on IFN␤ transcript stability was assessed. The data depicted in Fig. 4B suggest that in macrophages neither MK2 nor MK3 significantly influences IFN␤ transcript stability induced by LPS treatment.
Corroborating the above conclusions, combined inactivation of MK2 and MK3 by pretreatment of human or mouse macro-phages (Fig. 5, A and B) with the p38 MAPK inhibitor SB203580 strongly inhibits LPS-induced IL-10 expression, whereas the induction of IFN␤ expression is not affected (Fig. 5, C and D). Moreover, in line with the findings in MK2/3 Ϫ/Ϫ macrophages (Fig. 2B), LPS-inducible STAT3 activation is not significantly affected in SB203580-treated macrophages (Fig. 5, A and B), although IL-10 expression is inhibited.

IRF3 Protein Expression and IRF3 Activation in Response to LPS Is Impaired in MK2-deficient but Not in MK2/3-deficient
Macrophages-The observations described above suggest that MK2/3-mediated regulation of LPS-induced IFN␤ gene expression occurs on the level of transcription. To further understand the underlying molecular mechanisms, we asked whether negative regulation of IFN␤ expression in MK2-deficient macrophages is due to impairment of signaling events, which are essential for the transcriptional regulation of IFN␤ gene expression. We focused on the two components IRF3 and NFB, which are essential parts of the transcriptional complex that regulates IFN␤ gene transcription (22)(23)(24). Fig. 6 (A and B) demonstrates that the activating phosphorylation of IRF3 at serine residue 396 is strongly impaired in MK2 Ϫ/Ϫ macrophages. To some extent, this down-regulation of IRF3 phos-

FIGURE 5. Combined inactivation of MK2 and MK3 by blocking p38 MAPK activity results in inhibition of LPS-induced IL-10 expression, whereas expression of IFN␤, as well as STAT3 activation, is not affected.
Because of the lack of phosphospecific antibodies specifically recognizing MK3-Thr 200 phosphorylated at threonine, activation of MK3 was analyzed in human macrophages because the antibody used to detect MK2-Thr 222 also recognizes the human but not mouse MK3 in its phosphorylated state. A and B, human PBMC (A) and murine bone marrow-derived macrophages (B) were generated and pretreated for 16 h with 1 M SB203580 and subsequently stimulated with 100 ng/ml LPS for the times indicated. Total protein lysates were prepared and separated on SDS page. The blots were analyzed using antibodies specifically recognizing STAT3-Tyr 705 , MK2-Thr 222 , or antibodies recognizing total protein of STAT3, MK2, MK3, and GAPDH. C and D, bone marrow-derived macrophages generated from wild type mice were pretreated with 1 M SB203580 for 16 h. Thereafter, the cells were stimulated with 100 ng/ml LPS for the times indicated. Total RNA extracts were prepared and subjected to RT-PCR for IFN␤ mRNA (C) and IL-10 mRNA expression (D). Comparable results were obtained from the analysis of LPS-induced IFN␤ and IL-10 mRNA expression in SB203580-pretreated human PBMC-derived macrophages (data not shown). Semiquantitative PCR results were obtained using the ⌬C T method. Cytokine mRNA was normalized to SDHA, and relative mRNA levels were expressed as fractions of the normalized value of the control, which was set to 100%. The data are presented as the means plus S.E. (n ϭ 3). p values smaller than 0.05 were considered significant. phorylation in MK2 Ϫ/Ϫ macrophages is due to reduced IRF3 protein expression (Fig. 6, A and B). In contrast, ablation of MK3 had almost no effect on IRF3 expression and/or its activation (Fig. 6C). In turn, ablation of both MK2 and MK3 was able to override the effect of the single MK2 deficiency and resulted in IRF3 protein levels and IRF3 phosphorylation that were almost comparable with wild type macrophages, as illustrated in Fig. 6 (A and B). These data suggest that in the absence of MK2, the sole presence of MK3 negatively influences IRF3 protein expression and its activation and that under normal conditions MK2 precludes MK3 from mediating these negative regulatory effects. To prove that MK3 indeed mediates negative regulatory effects on IRF3 function in the absence of MK2, MK3 was stably reconstituted in MK2/3 Ϫ/Ϫ macrophages by retroviral gene transfer. In support of the proposed inhibitory activity of MK3, its reconstitution in MK2/3 Ϫ/Ϫ macrophages results in the restoration of the MK2 Ϫ/Ϫ phenotype with reduced IRF3 protein levels and decreased phosphorylation of IRF3 (Fig. 6, D and E). In contrast to the effect of the reconstituted MK3 expression, the stable reconstitution of MK2 expression in MK2/3 Ϫ/Ϫ macrophages does not significantly affect IRF3 protein levels and even increases phosphorylation or IRF3 (Fig. 6E). This indicates that these two kinases exert different roles on the regulation of IRF3 function.
MK2 Prevents MK3 from Impeding Nuclear Translocation of NFB p65-Apart from the involvement of IRF3, transcriptional regulation of the IFN␤ gene also requires activation and nuclear translocation of NFB. To assess whether MK2 and MK3 regulate NFB activation, we analyzed p65 nuclear translocation in wild type, MK2 Ϫ/Ϫ , and MK2/3 Ϫ/Ϫ macrophages. Notably, in MK2-deficient macrophages, p65 nuclear translocation is reduced and occurs with substantial delay when compared with wild type controls (Fig. 7, A and B). This alteration leads to a desynchronization of p65-mediated signals in comparison with the different other signaling events elicited by LPS, such as the activation of IRF3, and therefore might be responsible for disturbed transcriptional regulation of gene expression. In this context, it is interesting to note that not only the strength but also the kinetics of NFB activation has been suggested to be critical for transcriptional regulation of gene expression (25,26). Consistently, p65 is almost undetectable in nuclear extracts isolated from MK2 Ϫ/Ϫ macrophages when analyzed after 15 (Fig. 7, A and B) or 20 min (Fig. 7C). As shown in Fig. 7 (A and C), the delay in nuclear translocation of p65 was almost completely reversed upon additional ablation of the MK3 gene in MK2/3 Ϫ/Ϫ macrophages. These data suggest that in macrophages, MK3 counteracts nuclear translocation of p65 in response to LPS in the absence of MK2. Consistent with this, reintroduced MK3, but not MK2, in MK2/3 Ϫ/Ϫ , macrophages restores the phenotype of MK2 Ϫ/Ϫ macrophages with impaired nuclear translocation of p65 (Fig. 7D).
In MK2-deficient Macrophages, Delayed Degradation of IB␤ Is Responsible for Impaired Expression of IB␣-Nuclear NFB regulates the expression of a multitude of different genes, either as the leading regulatory factor or as a part of a transcriptional complex that essentially requires additional factors to gain optimal functionality. One of the target genes that is primarily regulated by NFB is its own inhibitor IB␣, which is induced as part of an autoregulatory feedback loop (27,28). Consequentially, as a result of impaired NFB activation, expression of IB␣ is strongly decreased in nonstimulated MK2 Ϫ/Ϫ macrophages when compared with wild type (Figs. 7A and 8, A and C) and also in MK3-reconstituted MK2/3 Ϫ/Ϫ macrophages when compared with GFP control cells (Fig. 8B). The observation that reintroduction of MK3 into MK2/3 Ϫ/Ϫ macrophages also results in a down-regulation of IB␣ expression again emphasizes that MK3 exerts negative regulatory effects on NFB-dependent regulation of gene expression in the absence of MK2.
In contrast to IB␣, IB␤ expression is enhanced in MK2 Ϫ/Ϫ (Figs. 7A and 8, C and D) and MK2/3 Ϫ/Ϫ macrophages (Figs. 7A and 8D) when compared with wild type macrophages. Moreover, LPS-inducible degradation of IB␤ is substantially delayed in MK2 Ϫ/Ϫ macrophages when compared with WT and MK2/3 Ϫ/Ϫ macrophages (Figs. 7A and 8D). Thereby, in MK2-deficient macrophages, the time course of IB␤ degradation bridges the time period when IB␣ is absent in the cytoplasm and matches to the delayed appearance of p65 within the nucleus in MK2 Ϫ/Ϫ macrophages (Fig. 7A). According to this, co-immunoprecipitation experiments using cytoplasmic lysates indicate that in contrast to wild type and MK2/3 Ϫ/Ϫ FIGURE 7. MK3 counteracts p65 NF-B nuclear translocation in the absence of MK2. A and C, WT M⌽, MK2 Ϫ/Ϫ M⌽, and MK2/3 Ϫ/Ϫ M⌽ were used. D, MK2/3 Ϫ/Ϫ M⌽ were stably transfected with a vector co-expressing MK2 or MK3 and GFP via an IRES or with the same vector expressing GFP for control. The cells were treated with LPS (100 ng/ml) for the time periods indicated, and nuclear extracts (NE) and/or cytosolic lysates (CL) were prepared as outlined under "Experimental Procedures." 40 g of protein were analyzed using antibodies specifically recognizing the p65 subunit of the NFB complex, IB␣, and IB␤ as indicated. For fractionation control of nuclear extracts, expression of lamin A/C was analyzed. GAPDH expression was determined for fractionation control of cytosolic lysates. B, p65 nuclear translocation was quantified by densitometric evaluation of the blots using the TotalLab TL1000 software (version 2006) from Nonlinear Dynamics Ltd. and normalized to the levels of lamin A/C. The data are presented as the means plus S.E. (n ϭ 3). macrophages, the binding of p65 to IB␤ and the LPS-induced degradation of IB␤ is prolonged in MK2 Ϫ/Ϫ macrophages (Fig. 8D). Hence, the data suggest that impaired early nuclear translocation of p65 in MK2-deficient macrophages is due to delayed degradation of IB␤, which interacts with the same spectrum of Rel proteins as IB␣ (29). This assumption is further substantiated by the observation that in MK2 Ϫ/Ϫ macrophages, expression of the NFB target gene IB␣ is almost completely restored back to those observed in wild type macrophages if enhanced IB␤ expression (Fig. 8C, third and  fourth lanes) is knocked down back to wild type protein levels using IB␤-specific siRNA (Fig. 8C, compare first and second  lanes with fifth and sixth lanes).

DISCUSSION
In macrophages, STAT3 is considered to be the major mediator of anti-inflammatory effects (3)(4)(5). Its sustained activation in response to LPS is the result of a complex and yet only partially understood feedback loop (3-6) involving activation of IFNAR and IFNAR-dependent release of IL-10 (7, 30). By analyzing the role of MK2 and MK3 for LPS-inducible STAT3 activation in macrophages, the present study reveals unexpected and novel details on the function of MK3, which so far appeared as closely related, cooperatively acting isoenzyme of MK2 with an identical recognition sequence (10,16). The present manuscript indicates for the first time that MK2 and MK3 also have different regulatory roles, with MK2 regulating gene expression by preventing MK3-mediated negative regulatory effects on LPS-induced signaling (for a schematic summary see Fig. 9).
The data suggest that in macrophages MK2 is important for delayed STAT3 activation in response to LPS (Figs. 2, A and B, and A) because it controls IFN␤ expression (Fig. 4A), which in turn is required for IFNAR-dependent activation of STAT3 (Fig. 3). Thereby, MK2 does not directly control IFN␤ gene expression (Fig. 4, A and B) but rather prevents MK3 from inhibiting signaling events (Figs. 6 and 7) essential for induction of IFN␤ gene expression (22)(23)(24). Thus, in the absence of MK2, MK3 is demonstrated to be responsible for impaired IRF3 function (Fig. 6) and inhibition of nuclear translocation of the p65 subunit of NFB (Fig. 7), both of which are reported to be crucial for transcriptional control of IFN␤ gene expression (22)(23)(24).
In line with the data reported herein, impaired p65 nuclear translocation and reduced p65 NF-B DNA binding capacity as a result of MK2 depletion has recently also been demonstrated in human umbilical endothelial cells (31). However, the underlying molecular mechanisms identified to be responsible for impaired nuclear translocation of NFB differ substantially between human umbilical endothelial cells and macrophages; thus, in human umbilical endothelial cells, impaired nuclear translocation of p65 has been reported to be due to an accelerated rescue of IB␣ protein expression that results in an enhanced and premature removal of p65 from the nucleus (31). FIGURE 8. In MK2 ؊/؊ macrophages, delayed IB␤ degradation is responsible for impaired IB␣ expression. A, total RNA extracts were prepared from untreated WT M⌽, MK2 Ϫ/Ϫ M⌽, and MK2/3 Ϫ/Ϫ M⌽ and subjected to RT-PCR for IB␣ mRNA expression. Semiquantitative PCR results were obtained using the ⌬C T method. IB␣ mRNA was normalized to SDHA, and relative mRNA levels were expressed as fractions of the normalized value of the control, which was set to 100%. The data are presented as the means plus S.E. (n ϭ 3). The statistics were calculated as outlined under "Experimental Procedures." p values smaller than 0.05 were considered significant. B, MK2/3 Ϫ/Ϫ M⌽ were stably transfected with a vector co-expressing MK2 or MK3 and GFP via an IRES or with the same vector expressing GFP for control and treated with LPS (100 ng/ml) as indicated. Thereafter, cytoplasmic extracts were prepared, and 40 g of protein were analyzed by immunoblot using an antibody specifically recognizing IB␣. For loading control, expression of GAPDH was determined. C, MK2 Ϫ/Ϫ macrophages were transfected with IB␤-specific siRNA (IB␤ siRNA) or scrambled siRNA for control. 48 h after transfection macrophages were stimulated for 15 min with 100 ng/ml LPS or left untreated for control, and cytosolic lysates (CL) were prepared and analyzed for IB␣, IB␤ MK2, and GAPDH for loading control by Western blot analysis. D, WT M⌽, MK2 Ϫ/Ϫ M⌽, and MK2/3 Ϫ/Ϫ M⌽ were treated with 100 ng/ml LPS as indicated. After preparation, cytoplasmic extracts were incubated with IB␤ antibody, and co-immunoprecipitation (IP) was performed as outlined under "Experimental Procedures." Precipitates were separated on 8% SDS page and analyzed after blotting using antibodies specifically recognizing p65 or IB␤.
Contrariwise, in macrophages ablation of the MK2 gene unmasks negative regulatory properties of MK3 (Fig. 7), resulting in delayed and impaired nuclear translocation of p65 and a significant reduction of IB␣ transcript and protein (Figs. 7, A  and B, and 8, A and B) levels. Thereby, it is likely that downregulation of IB␣ expression reflects impaired NFB activation, because it is known to be NFB-dependently induced as a part of an autoregulatory feedback loop (27).
Of note, in MK2 Ϫ/Ϫ macrophages, delayed nuclear translocation of p65 and impaired expression of p65 target genes such as IB␣ is accompanied by an enhanced expression and a decel-erated LPS-induced degradation of IB␤ (Fig. 7A). IB␤ is reported to interact with the same spectrum of Rel proteins as IB␣ (28,29) and is able to retain p65 in the cytoplasm of unstimulated cells (32) as also demonstrated herein (Fig. 8D). Depletion of IB␤ results in increased basal NFB DNA binding activity, and its expression inhibits NFB activation (32). Moreover, studies on cell lines deficient for IB␣ and IB⑀ with IB␤ as the most prominent member of the IB family, suggest that in contrast to IB␣, IB␤ mediates delayed but sustained activation of NFB (33). Therefore, one may conclude that in MK2-deficient macrophages, IB␤ is responsible for impaired FIGURE 9. Schematic summary of the regulation of LPS-induced STAT3 activation by MK2 and MK3. The present work suggests that in macrophages, MK2 regulates LPS-induced expression of IFN␤ by preventing MK3 from exerting negative regulatory effects on NFB-and IRF3-dependent signaling. Both IRF3 and the p65 subunit of NFB are essential components of the transcriptional complex that mediates enhanced transcription of the IFN␤ gene in response to LPS (22)(23)(24). As indicated by the data, MK2 maintains IRF3-dependent LPS signaling because it deters MK3 from impeding IRF3 protein expression and its activation in response to LPS (step 1 in the scheme). Furthermore, MK2 warrants timely and unimpaired nuclear translocation of p65 by antagonizing inhibitory effects of MK3 (step 2), which causes a delayed and diminished LPS-induced nuclear translocation of p65 in the absence of MK2. Thereby MK2 assures accurate NFB nuclear translocation by controlling basal protein levels of IB␤ and by preventing MK3 from impeding its degradation (step 2). Hence, MK2 deficiency results in a MK3-dependent desynchronization of the LPS-induced activation of important components of the transcriptional complex that enhances transcription of the IFN␤ gene (step 3) and therefore inhibits IFN␤ expression. Apart from IFN␤, p65 also controls the expression of other NFB target genes such as IB␣ (step 4), which is part of an autoregulatory feedback loop. As a consequence of the hampered NFB activation in MK2 Ϫ/Ϫ macrophages, IB␣ gene expression is likewise impaired. Previous reports (7) and the data provided herein indicate that LPS-induced IL-10 expression and IL-10-induced activation of STAT3 requires activation of the IFNAR (step 5), which is also capable of directly mediating IFN␤-induced STAT3 activation (step 6). Hence, MK2 also controls LPS-mediated activation of STAT3 in an indirect way as it ensures IFN␤ expression. In addition, the data indicate that MK2 (independent from MK3) is required for the transcript stabilization of IL-10 (step 7) but not of IFN␤. This may further explain the observation that additional deletion of the MK3 gene in MK2/3 Ϫ/Ϫ macrophages reconstitutes LPS-induced expression of IFN␤ but not of IL-10. Interestingly, in MK2/3 Ϫ/Ϫ macrophages rescued IFN␤ expression, and subsequent IFNAR activation is sufficient to mediate LPS-induced activation of STAT3 independent from IL-10, which is not restored, indicating that under defined conditions IFNAR activation alone is sufficient to mediate STAT3 activation in response to LPS. Corresponding to this, combined inhibition of MK2 and MK3 activation by blocking their common upstream activator p38 MAPK using the compound SB203580, supposed to specifically inhibit catalytic activity of p38 MAPK , does not inhibit LPS-induced IFN␤ expression and subsequent STAT3 activation, although it inhibits IL-10 expression. and delayed activation of p65 and down-regulation of IB␣ expression. This assumption is supported by the observation that knockdown of IB␤ expression in MK2 Ϫ/Ϫ macrophages by specific siRNA (Fig. 8C) restores expression of IB␣ back to protein levels observed in wild type macrophages. However, in contrast to IB␣, this restoration of NFB-dependent gene expression by suppressing IB␤ expression was not sufficient to rescue IFN␤ expression in MK2 Ϫ/Ϫ macrophages (data not shown). This indicates that re-establishment of NFB-mediated signaling alone would not be sufficient to reconstitute LPS-inducible IFN␤ expression in MK2 Ϫ/Ϫ macrophages because this requires the activation of additional transcription factors, such as IRF3 (24).
Studies using IB␤ knock-out animals indicated that IB␤ has a dual function in regulation of inflammatory gene expression. Thus, apart from cytoplasmic sequestration of NFB in unstimulated cells and NFB release during the early stages of stimulation, IB␤ also plays a crucial role as a transcriptional regulator of inflammatory cytokine expression in vivo (32,34). In this context, the previously described hypo-or unphosphorylated form of IB␤ seems to be particularly important. Upon stimulation, this form is newly synthesized (35) and forms a transcriptional active DNA-binding complex with p65 and c-Rel (32). This complex has been suggested to be required for expression of inflammatory cytokines, such as TNF␣, IL-1␤, IL-6, and IL-12 (32,34). Whether IB␤ also plays a role for the transcriptional regulation of type I interferon expression is unknown. However, in MK2-deficient macrophages basal IB␤ expression is up-regulated (Figs. 7A and 8C), but not diminished, and therefore rather represents a situation of basal IB␤ overexpression accompanied by impaired NFB activation (32) and not an IB␤ knock-out situation with basal NFB activation being up-regulated.
Apart from p65 activation, the activation of IRF3 is also impaired in MK2 Ϫ/Ϫ macrophages (Fig. 6). In analogy to p65, impaired function of IRF3 in the absence of MK2 is demonstrated to be also due to negative regulatory effects of MK3. Accordingly, impaired IRF3 function can be almost completely rescued by additional ablation of the MK3 gene in MK2/3 Ϫ/Ϫ macrophages (Fig. 6). Thereby, it is likely that reduced activation of IRF3 in MK2 Ϫ/Ϫ macrophages is to a major degree due to down-regulation of IRF3 protein levels. However, despite substantial efforts, we were not able to clarify the molecular mechanisms responsible for down-regulation of IRF3 protein levels in MK2 Ϫ/Ϫ macrophages. Our own data demonstrate that reduction of IRF3 protein expression observed in MK2 Ϫ/Ϫ macrophages is due neither to impaired transcription nor to reduced IRF3 transcript stability and does not involve enhanced MG132-sensitive proteasomal degradation, caspasemediated protein degradation or bafilomycin-sensitive proteolysis (data not shown). Furthermore, pull-down assays and co-immunoprecipitation studies indicate that, in contrast to p38 MAPK , which has been demonstrated to be stabilized by its interaction with MK2 (36), IRF3 interacts with neither MK2 nor MK3 (data not shown). Hence, it is also unlikely that regulation of IRF3 protein stability based on direct protein/protein interaction with either MK2 or MK3 plays a role. Protein kinase assays, using recombinant IRF3 as a substrate, further suggest that LPS-inducible phosphorylation of IRF3 is TBK1-dependent but occurs independently of MK2 (data not shown).
As schematically summarized in Fig. 9, the data provide strong evidence that MK2 controls LPS-inducible IFN␤ gene expression and subsequent IFNAR-mediated activation of STAT3 by neutralizing negative regulatory effects of MK3 on LPS-induced p65 and IRF3-mediated signaling. The data further indicate that in MK2/3 Ϫ/Ϫ macrophages, IFNAR-dependent STAT3 activation occurs independently from IL-10, because in contrast to IFN␤, impaired IL-10 expression is not restored upon additional deletion of MK3 in MK2/3 Ϫ/Ϫ macrophages. These differences between IFN␤ and IL-10 are suggested to be due to the fact that the IL-10 transcript requires the presence of MK2 for stabilization (Fig. 4D), which is in line with the regulation previously reported for other transcripts such as IL-6 (37). In contrast to IL-10, MK2 deficiency has no effect on the stability of the IFN␤ transcript (Fig. 4B), suggesting that regulation of IFN␤ by MK2 and MK3 does not occur on the level of transcript stability. Because in MK2/3 Ϫ/Ϫ macrophages IFN␤ expression, but not IL-10 expression, is restored, the data further indicate that in MK2/3 Ϫ/Ϫ macrophages, LPS-inducible activation of STAT3 occurs independently from IL-10. The fact that LPS indeed is able to activate STAT3 IL-10-independently in the absence of MK2 and MK3 activity is further substantiated by the finding that combined inactivation of MK2 and MK3 by blocking p38 MAPK activity does not affect STAT3 activation (Fig. 5, A and B), although LPS-induced expression of IL-10 ( Fig. 5D) but not of IFN␤ (Fig. 5C) is strongly affected. Thereby, it is likely that in MK2/3 Ϫ/Ϫ macrophages, activation of IFNAR by IFN␤ is sufficient to mediate LPS-induced STAT3 activation IL-10-independently, because IFN␤-induced STAT3 activation is not impaired in MK2 Ϫ/Ϫ or MK2/3 Ϫ/Ϫ macrophages (Fig. 2E).