Interactions of the Human LIP5 Regulatory Protein with Endosomal Sorting Complexes Required for Transport*♦

Background: LIP5 helps regulate the membrane fission and recycling activities of the ESCRT pathway. Results: Biochemical and structural studies reveal how LIP5 binds different ESCRT-III proteins. Conclusion: ESCRT-III proteins bind independently to different LIP5 sites, and the LIP5-CHMP5 structure reveals a novel interaction mode. Significance: The results help explain how LIP5 can activate VPS4 ATPases to act on ESCRT-III filaments. The endosomal sorting complex required for transport (ESCRT) pathway remodels membranes during multivesicular body biogenesis, the abscission stage of cytokinesis, and enveloped virus budding. The ESCRT-III and VPS4 ATPase complexes catalyze the membrane fission events associated with these processes, and the LIP5 protein helps regulate their interactions by binding directly to a subset of ESCRT-III proteins and to VPS4. We have investigated the biochemical and structural basis for different LIP5-ligand interactions and show that the first microtubule-interacting and trafficking (MIT) module of the tandem LIP5 MIT domain binds CHMP1B (and other ESCRT-III proteins) through canonical type 1 MIT-interacting motif (MIM1) interactions. In contrast, the second LIP5 MIT module binds with unusually high affinity to a novel MIM element within the ESCRT-III protein CHMP5. A solution structure of the relevant LIP5-CHMP5 complex reveals that CHMP5 helices 5 and 6 and adjacent linkers form an amphipathic “leucine collar” that wraps almost completely around the second LIP5 MIT module but makes only limited contacts with the first MIT module. LIP5 binds MIM1-containing ESCRT-III proteins and CHMP5 and VPS4 ligands independently in vitro, but these interactions are coupled within cells because formation of stable VPS4 complexes with both LIP5 and CHMP5 requires LIP5 to bind both a MIM1-containing ESCRT-III protein and CHMP5. Our studies thus reveal how the tandem MIT domain of LIP5 binds different types of ESCRT-III proteins, promoting assembly of active VPS4 enzymes on the polymeric ESCRT-III substrate.

The endosomal sorting complex required for transport (ESCRT) pathway remodels membranes during multivesicular body biogenesis, the abscission stage of cytokinesis, and enveloped virus budding. The ESCRT-III and VPS4 ATPase complexes catalyze the membrane fission events associated with these processes, and the LIP5 protein helps regulate their interactions by binding directly to a subset of ESCRT-III proteins and to VPS4. We have investigated the biochemical and structural basis for different LIP5-ligand interactions and show that the first microtubule-interacting and trafficking (MIT) module of the tandem LIP5 MIT domain binds CHMP1B (and other ESCRT-III proteins) through canonical type 1 MIT-interacting motif (MIM1) interactions. In contrast, the second LIP5 MIT module binds with unusually high affinity to a novel MIM element within the ESCRT-III protein CHMP5. A solution structure of the relevant LIP5-CHMP5 complex reveals that CHMP5 helices 5 and 6 and adjacent linkers form an amphipathic "leucine collar" that wraps almost completely around the second LIP5 MIT module but makes only limited contacts with the first MIT module. LIP5 binds MIM1-containing ESCRT-III proteins and CHMP5 and VPS4 ligands independently in vitro, but these interactions are coupled within cells because formation of stable VPS4 complexes with both LIP5 and CHMP5 requires LIP5 to bind both a MIM1-containing ESCRT-III protein and CHMP5. Our studies thus reveal how the tandem MIT domain of LIP5 binds different types of ESCRT-III proteins, promoting assembly of active VPS4 enzymes on the polymeric ESCRT-III substrate.
The endosomal sorting complex required for transport (ESCRT) 3 pathway mediates membrane remodeling and fission events required for intraluminal vesicle formation at multivesicular bodies, the abscission stage of cytokinesis, and the budding of many enveloped viruses, including HIV-1 (reviewed in Refs. [1][2][3][4][5][6][7][8][9]. ESCRT factors are recruited to their different sites of action by sequential protein-protein and protein-membrane interactions that are initiated by membrane-specific adaptors such as the HRS-STAM complex (multivesicular body vesicle formation), CEP55 (cytokinesis), and retroviral Gag proteins (virus budding). These adaptors then bind and recruit earlyacting ESCRT factors such as ESCRT-I, ESCRT-II, and ALIX, which in turn recruit downstream ESCRT-III and VPS4 ATPase complexes.
The actual membrane fission events appear to be catalyzed by the concerted activities of the ESCRT-III and VPS4 complexes. Humans express 12 related ESCRT-III-like proteins, which can be subdivided into eight different subfamilies, termed CHMP1-7 (charged multivesicular body proteins 1-7) and IST1. The CHMP1, CHMP2, and CHMP4 subfamilies each have multiple related members, which carry letter designations (CHMP1A and CHMP1B, etc.). The different ESCRT-III subunits share a common architecture, comprising a conserved core domain that mediates membrane binding and filament formation and a C-terminal tail that can either fold back on the core to autoinhibit polymerization or open to expose proteinbinding sites (reviewed in Ref. 10). The core domain is a fourhelix bundle consisting of a long helical hairpin and two smaller helices that pack against the open end of the hairpin (10 -13). The C-terminal tails can vary considerably in sequence and length but commonly contain sequence elements termed microtubule-interacting and trafficking (MIT)-interacting motifs (MIMs) that can bind the MIT domains found in VPS4 and many other ESCRT-III-associated proteins (reviewed in Ref. 14). Although the mechanistic details remain to be determined, current models generally posit that ESCRT-III proteins form membrane-bound filaments that recruit other cofactors and draw opposing membranes together to promote fission (15)(16)(17).
The energy for ESCRT-mediated vesicle formation is ultimately provided by the VPS4 ATPase, which is expressed as a single protein in yeast (Vps4p) and two related VPS4 proteins in humans and other mammals (VPS4A and VPS4B/SKD1) (reviewed in Ref. 18). VPS4 ATPases function late in the ESCRT pathway to release assembled ESCRT-III subunits from the membrane (19 -22). VPS4 enzymes arrive immediately prior to the membrane fission step of virus budding and abscission (8,(23)(24)(25)(26) and may play an active role in the fission process, possibly by remodeling or reorganizing the membrane-bound ESCRT-III filaments. Like other AAA (ATPases associated with diverse cellular activites) ATPases, VPS4 proteins can form hexameric rings, and the active enzyme appears to be a singlering hexamer, 4 although double-ring dodecamers and other higher order oligomeric assemblies can also form in vitro (19,(27)(28)(29)(30)(31). Each VPS4 subunit comprises an N-terminal MIT domain, an ATPase cassette that contains canonical large and small AAA-ATPase domains, and a ␤-domain that is inserted within the small ATPase domain. The MIT domain binds MIM elements within different ESCRT-III substrates, the AAA-ATPase cassette mediates nucleotide hydrolysis and ring formation, and the three-stranded sheet of the ␤-domain interacts with the activator protein LIP5 (yeast Vta1p) (18).
Comprehensive genetic and biochemical studies in yeast have provided a model for Vps4p cofactor recruitment in which the protein is initially present in the cytoplasm as a lower order multimer (either a monomer or dimer) that is recruited to core ESCRT-III polymers with the help of the Did2p-Ist1p complex. This assembly then recruits Vta1p, which helps to promote assembly of the active Vps4p enzyme (33). It is not yet clear precisely how Vps60p/CHMP5 fits into this scheme, but a leading model is that Vps60p acts through Vta1p very late in the process to help stimulate Vps4p to recycle ESCRT components back into the cytoplasm (34). Consistent with this idea, recom-binant Vps60p can stimulate Vps4p ATPase activity in a Vta1pdependent fashion. Vta1p is required for Vps60p endosomal localization, but the converse is not true, and indeed, Vps60p antagonizes Vta1p endosomal localization (34). In mammalian cells, however, CHMP5 and LIP5 form stable complexes in the cytoplasm, and the two proteins are therefore likely to be recruited together in this case (53). CHMP5 activities appear to be more important for some ESCRT-dependent processes than others because siRNA depletion of CHMP5 inhibits multivesicular body sorting, but does not inhibit HIV-1 budding (44).
A remarkably complex network of different MIM-MIT interactions plays a critical role in recruiting different effector proteins to the surface of polymerized ESCRT-III filaments. The 12 different human ESCRT-III subunits contain distinct classes of MIMs that can be displayed singly (as in CHMP5) (34,50) or in tandem (as in IST1) (42) and can even bind differently to different MIT domains (as in CHMP1B) (36,54). Moreover, humans express more than a dozen different MIT domain-containing proteins (14,(55)(56)(57), whose MIT domains can be arrayed either singly (as in VPS4 enzymes) or in tandem (as in LIP5) (58). Although MIT domains are simple three-helix bundles (59,60), they can engage their MIM ligands in a variety of different ways. To date, four different classes of MIM elements have been characterized structurally: MIM1 motifs form short amphipathic helices that bind in the groove between MIT helices 2 and 3 (35,36), MIM2 motifs are extended strands that bind in the groove between MIT helices 1 and 3 (37,61), MIM3 motifs are longer amphipathic helices that bind in the same groove (54), and MIM4 motifs are related to MIM1 motifs but form a more extended helix and make more polar interactions (62). In all of these cases, the MIM ligand binds along a MIT groove in an orientation that is parallel to the first MIT helix. Despite these advances in our understanding of the molecular basis for specific MIM-MIT interactions, we do not yet have a complete inventory of all possible MIM-MIT interactions or a full understanding of the "code" that dictates how different MIMs select their specific MIT domain-binding partners.
LIP5 performs its central regulatory function within the ESCRT pathway by binding ESCRT-III proteins through its N-terminal tandem MIT domain and by binding VPS4 ATPases through its C-terminal VSL (Vta1/SBP1/LIP5) domain (see Fig. 1A) (45,48). These interactions allow LIP5 to coordinate ESCRT-III and VPS4 activities both during normal cellular homeostasis and also apparently in response to interferon signaling during innate immune responses. In the latter case, Leis and co-workers (63,64) have reported that covalent modification of CHMP5 and other ESCRT-III proteins with the ubiquitin-like protein ISG15 blocks the LIP5-CHMP5 and LIP5-VPS4 interactions and thereby inhibits enveloped viruses from using the ESCRT pathway to bud from cells. To define better how LIP5 can mediate its different regulatory functions, we have characterized how LIP5 interacts with CHMP5 and other ESCRT-III proteins and determined the interdependence of different LIP5-ligand interactions both in vitro and in cells.

Expression Constructs and Plasmids
Bacterial and mammalian expression constructs and antibodies are provided in supplemental Table 1.

LIP5 and CHMP5 Expression
LIP5 and CHMP5 proteins with N-terminal GST or His 10 (hereafter referred to as His) affinity tags (supplemental Table  1) were expressed in BL21-CodonPlus(DE3)-RIPL Escherichia coli (Agilent). Cells were grown in ZYP-5052 autoinduction medium (65) at 37°C for 5 h, transferred to 19°C for 16 -24 h, harvested by centrifugation at 4000 ϫ g for 10 min, and stored frozen at Ϫ80°C. Immobilized GST fusion proteins used in biosensor binding studies were captured from crude supernatants onto the biosensor chips using immobilized anti-GST antibodies, whereas all other GST and all His fusion proteins were purified to homogeneity as described below.
For GST fusion proteins, 10 g of cell paste was thawed, resuspended in 50 ml of GST lysis buffer (50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mg/ml lysozyme, 0.125% deoxycholate, 1 mM DTT, and protease inhibitors (aprotinin, leupeptin, pepstatin, and PMSF)), lysed by sonication, and clarified by centrifugation at 15,000 rpm (Beckman JA-20 rotor) for 45 min. Supernatants were filtered through 0.45-m syringe filters (Corning) and loaded onto 5-ml glutathione-Sepharose columns (GE Healthcare Life Sciences). The matrices were washed with 20 column volumes of GST wash buffer (50 mM Tris-HCl (pH 8.0), 150 mM NaCl, and 1 mM DTT), 20 column volumes of high salt GST wash buffer (GST wash buffer plus 500 mM NaCl), and 20 column volumes of GST wash buffer. Bound proteins were eluted with 60 ml of GST wash buffer plus 20 mM reduced L-glutathione (Sigma). For His fusion proteins, cell pellets were thawed, resuspended, and lysed as described above but in His lysis buffer (50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mg/ml lysozyme, 0.125% deoxycholate, 25 mM imidazole, and protease inhibitors). Clarified lysates were filtered and loaded onto a 5-ml nickel-nitrilotriacetic acid-agarose affinity column (Qiagen). Matrices were washed with 20 column volumes of His buffer (50 mM Tris-HCl (pH 8.0), 150 mM NaCl, and 25 mM imidazole), 20 column volumes of high salt His buffer (His buffer plus 500 mM NaCl), and 20 column volumes of His buffer. Bound proteins were eluted with 60 ml of His buffer plus 750 mM imidazole.
Eluted GST and His fusion proteins were processed to remove the affinity tags. Fusion proteins were dialyzed against 2 liters of proteolysis buffer I (50 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 5 mM EDTA) for 4 h, tobacco etch virus or Pre-Scission protease was added at a 1:150 enzyme:substrate ratio, and the reactions were dialyzed for an additional 12-15 h against 2 liters of proteolysis buffer II (50 mM Tris-HCl (pH 8), 100 mM NaCl, 0.5 mM EDTA, and 1 mM DTT) at 4°C (PreScission protease) or room temperature (tobacco etch virus protease). Proteolytic processing was generally quantitative and left either two or three non-native residues at the N terminus (Gly-His (referred to hereafter as GH), tobacco etch virus; or Gly-Pro-His (referred to hereafter as GPH), PreScission protease), which are not included in our numbering schemes.
Processed LIP5 proteins were purified by ion exchange chromatography (LIP5(1-183) using Q-Sepharose and all other LIP5 proteins using SP-Sepharose; GE Healthcare Life Sciences) with elution gradients from 50 to 500 mM NaCl in 50 mM Tris-HCl (pH 8) and 1 mM DTT. Fractions containing LIP5 proteins were concentrated and purified to homogeneity by gel filtration using Superdex 75 columns (GE Healthcare Life Sciences). The buffer used for gel filtration was either 25 mM Tris-HCl (pH 8), 150 mM NaCl, and 1 mM DTT (proteins used for binding studies) or 20 mM sodium phosphate (pH 6.3), 100 mM NaCl, 0.1 mM EDTA, and 0.5 mM DTT (proteins used for NMR spectroscopy). These procedures yielded ϳ1 mol/liter of culture. Expected protein masses were confirmed by electrospray ionization mass spectrometry: GPH-LIP5(  20 mM imidazole) and lysed by sonication, and the lysate was clarified by centrifugation at 15,000 rpm (Beckman JA-20 rotor) for 45 min. Supernatants were filtered through 0.45-m syringe filters and loaded onto a 5-ml equilibrated nickel-nitrilotriacetic acid-agarose affinity column. The matrix was washed with 20 column volumes of denaturing lysis buffer, followed by 20 column volumes of denaturing lysis buffer without urea. His fusion proteins were eluted with 60 ml of 50 mM Tris-HCl (pH 8), 500 mM NaCl, and 750 mM imidazole and processed using tobacco etch virus protease as described for LIP5. Peptides were concentrated, dried, resuspended in 2 ml of aqueous solution containing 5% acetic acid and 30% acetonitrile, and purified by reverse-phase C 18

Biosensor Binding Assays
Biosensor binding experiments were performed as described previously (36). Briefly, GST-LIP5 and GST-CHMP5 proteins were expressed as described above. Cells from 10-ml cultures were resuspended in 4 ml of lysis buffer (50 mM Tris-HCl (pH 7.0), 300 mM NaCl, 5% (w/v) glycerol, 0.125% sodium deoxycholate, 5 mM 2-mercaptoethanol, 1 mg/ml lysozyme, and protease inhibitors) and sonicated, and the lysates were clarified by centrifugation for 30 min at 13,000 rpm in a microcentrifuge. GST-fused proteins were captured directly onto anti-GST antibody-derivatized CM5 sensor chips. Pure LIP5 proteins or CHMP1B, CHMP2A, CHMP3, CHMP4B, CHMP5, and CHMP6 or IST1 peptides were diluted to the designated concentrations in binding buffer (25 mM Tris-HCl (pH 7.2), 150 mM NaCl, 1 mM DTT, 0.1 mg/ml BSA, and 0.01% Tween 20) and injected in triplicate (50 l/min, 25°C), and binding data were collected. Binding constants were obtained by fitting the equilibrium responses to 1:1 binding models (see Fig. 6, B-E). Each binding isotherm was measured one to five times, and mean K D values Ϯ S.D. are reported as described in the legend to Fig. 6.

Fluorescence Binding Assays
Fluorescence anisotropy binding measurements were performed in Corning 384-well black plates on a Tecan Infinite 200 plate reader with excitation at 485 nm and detection at 535 nm. 60-l binding reactions (30 min, 25°C) contained 2-fold dilutions of LIP5 proteins and 250 pM dye-labeled CHMP5(139 -191)(A191C) peptide in binding buffer. K D values were obtained by fitting the increase in fluorescence anisotropy (⌬FA) with the 1: [LIP5]) 1/2 using KaleidaGraph (Synergy Software). Each binding isotherm was measured at least four times, and mean K D values Ϯ S.D. are reported.

Analytical Ultracentrifugation
Analytical ultracentrifugation data were collected at 4°C using a Beckman Optima XL-A centrifuge at a rotor speed of 11,000 rpm for 5.6, 11.2, and 22.4 M LIP5 in 25 mM Tris-HCl (pH 8.0), 150 mM NaCl, and 1 mM tris(2-carboxyethyl)phosphine. Data were globally fit to ideal single-species models with fixed or floating molecular masses (data not shown) using the nonlinear least squares algorithm in the HETEROANALYSIS software. Protein partial specific volumes and solvent densities were calculated with the program SEDNTERP (version 1.09).

NMR Data Collection and Resonance Assignment
NMR data for resonance assignments, residual dipolar couplings, and titrations were recorded on a Varian INOVA 600- DECEMBER 21, 2012 • VOLUME 287 • NUMBER 52

Structure Calculations
NMR structures were determined from NMR-derived distance constraints, and torsion angle constraints, and residual dipolar couplings. Interproton NOE intensities and coordinates were obtained from three-dimensional 1H-(13C,15N)-1H NOESY (60-ms mixing time) using SPARKY 3. A half-filtered three-dimensional 1H-(13C,15N)-1H NOESY (60-ms mixing time) was recorded on sample 3 and used to confirm intermolecular NOEs at the complex interface (see Fig. 8). Main chain phi and psi torsion angle ranges were estimated from 13 C␣ chemical shifts using TALOSϩ (69,70). Chemical shift analyses indicated that all prolines were in trans-conformations. Amide NH residual dipolar couplings ( 1 D NH ) were obtained from twodimensional 1H-15N HSQC, TROSY-HSQC, and in-phase anti-phase (IPAP) HSQC spectra in isotropic (samples 5 and 7) and aligned (samples 6 and 8) solutions. Dipolar couplings for residues in regular ␣-helical regions were included in calculations. NOEs were assigned using the automated NOE assignment module implemented in CYANA 3.0 (71,72). Table 1 summarizes the NMR-derived constraints and structure statistics. Structures were initially calculated using CYANA and refined using XPLOR-NIH 2.29. Distance, / torsion angle, and dipolar coupling constraint files were converted to XPLOR format using the software PDBSTAT (73). Stereochemical assignments from CYANA were retained, and the upper bounds of NOE distance constraints were increased by 10%. An improved simulated annealing protocol in XPLOR was implemented, with updated features (74), including a backbone hydrogen bond potential term (HBDB) and a torsion angle potential term (RAMA). Models were visualized and figures were prepared in PyMOL (DeLano Scientific). NMR constraints were deposited in the Biological Magnetic Resonance Bank (LIP5(MIT)2:18681 and LIP5-CHMP5:18682). 15 N-Labeled His-VPS4B(SAB) (small ATPase and beta; residues 300 -423) was expressed in M9 medium and affinity-purified, and the His affinity tag was removed using PreScission protease as described above for His-LIP5 proteins. The processed protein was dialyzed against 20 mM sodium phosphate (pH 6.3), 100 mM NaCl, 0.5 mM DTT, and 0.1 mM EDTA and further purified in the same buffer by gel filtration on a Superdex 75 column. Unlabeled His-LIP5(VSL) (residues 260 -307) was expressed in autoinduction medium and purified as described for VPS4B(SAB). Expected masses were confirmed by electrospray ionization mass spectrometry: 15  Amide chemical shifts were analyzed using NMRViewJ (One Moon Scientific, Inc.), and shift changes were calculated using ⌬HN ϭ (⌬H 2 ϩ (⌬N/6.5) 2 ) 1/2 . Equilibrium dissociation constants (K D ) were obtained by fitting to the 1:1 binding equa-

LIP5 Residues 1-163 Comprise a Tandem MIT Domain-We
used NMR spectroscopy to determine the solution structure of the N-terminal half of human LIP5 (residues 1-183) ( Fig. 1 and Table 1). The structure revealed that residues 1-163 comprise a globular domain that contains two MIT modules, as well as an ordered strand at the N terminus and a type I ␤-turn at the C terminus (Fig. 1B). This entire domain is hereafter generically referred to as LIP5(MIT) 2 , except in cases in which it is important to denote the precise position of the C terminus (see below). LIP5 residues 164 -170 form a poorly ordered mobile strand that extends beyond the final turn, and residues beyond 170 lack persistent structure.
Each MIT module within LIP5(MIT) 2 is a three-helix bundle, and the bundles are connected by a nine-residue linker that includes a short helix (H4, residues 77-80). The two MIT modules are oriented at approximately right angles and interact through an extensive interdomain interface formed by the first two helices of each module. As expected, LIP5(MIT) 2 resembles the analogous tandem MIT domain of the S. cerevisiae homolog Vta1p, and the six equivalent MIT helices overlay with a backbone atom (N, C␣, and C) root mean square deviation of 2.2 Å (Fig. 1C) (58). However, Vta1p(MIT) 2 lacks the LIP5(MIT) 2 N-terminal strand (residues 1-13) and the C-terminal turn (residues 156 -159). The N-terminal strand of LIP5(MIT) 2 folds back and packs into the groove between MIT1 helices 1 and 3, with key interactions made by Leu-4, Leu-7, Pro-8, Leu-10, and Pro-11. These interactions are reminiscent of an intermolecular crystal packing interaction seen in the crystal structure of the MIT domain from yeast Vps4p binding nonspecifically to a proline-rich sequence within Vps2p (35). Sequence alignments indicate that the N-terminal strand of human LIP5(MIT) 2 is well conserved in mammalian LIP5 proteins but can vary in sequence and length in other vertebrate and metazoan species (Fig. 2A).
MIM1 Elements from CHMP1B and Other ESCRT-III Proteins Bind LIP5 MIT1-Mutational, spectroscopic, and binding analyses were performed to determine how different ESCRT-III proteins can bind LIP5(MIT) 2 . Biosensor analyses were initially used to survey the binding of immobilized GST-LIP5(MIT) 2 to constructs that spanned MIMs from six different ESCRT-III proteins ( Fig. 3A and Table 2). The tightest binder was a construct that spanned the C-terminal region of CHMP1B (positions 169 -199; K D ϭ 2.9 Ϯ 0.4 M) (Fig. 3A, closed squares). This longer CHMP1B construct was initially tested because the C terminus of CHMP1B can bind different MIT domains either by forming a MIM1 motif, in which the C-terminal half of the final CHMP1B helix (residues ϳ180 -196) binds in the helix 2/3 groove (36), or by forming a MIM3 motif, in which a more extended helix (residues ϳ174 -196) binds in the helix 1/3 groove (54). As shown in Fig. 3B, a minimal CHMP1B construct that spanned just the MIM1 helix (CHMP1B(181-199)) bound LIP5(MIT) 2 with full affinity (K D ϭ 3.3 Ϯ 0.1 M) (Fig. 3B, compare open and closed diamonds). This dissociation constant is 10-fold tighter than the MIM1-type interaction made by CHMP1B with the VPS4A MIT domain (36) and ϳ4-fold tighter than the MIM3-type interaction made with the spastin MIT domain (54). Hence, the C-terminal CHMP1B MIM1 helix is an unusually good binding partner for LIP5(MIT) 2 .
Terminal  Table 2). Thus, LIP5(MIT) 2 can bind MIM1 elements from multiple ESCRT-III proteins but apparently cannot bind MIM2 elements. Our data generally agree well with a previous study (50), except that the equilibrium dissociation constant we measured for the CHMP1B constructs (ϳ3 M) was higher than that in the previous report (EC 50 ϭ 25 nM). In that case, however, the tighter binding measurement may have been enhanced by avidity effects because the experiment was configured so that dimeric LIP5 was binding to immobilized CHMP1B.
Mutational and spectroscopic analyses were performed to map the CHMP1B MIM1-binding site on LIP5(MIT) 2 . As noted above, MIMs can bind MIT domains in the grooves between helices 2 and 3 (the binding site for MIM1 and MIM4 motifs) or between helices 1 and 3 (the binding site for MIM2 and MIM3 motifs). LIP5(MIT) 2 therefore presents four candidate MIM-binding grooves (although binding in the helix 1/3 groove of the first LIP5 MIT module would require displacement of the N-terminal strand). The CHMP1B MIM1-binding site was mapped by testing binding to four different LIP5(MIT) 2 constructs, each of which carried point mutations in a different MIT binding groove. As shown in Fig. 3B, CHMP1B(169 -199) bound with similar affinities to wild-type LIP5(MIT) 2 and three mutant constructs. However, the LIP5(MIT) 2 mutation M64A within the helix 2/3 groove of MIT1 weakened CHMP1B(169 -199) binding by Ͼ50-fold.  (58)). DECEMBER 21, 2012 • VOLUME 287 • NUMBER 52

JOURNAL OF BIOLOGICAL CHEMISTRY 43915
This mutation had a similar inhibitory effect on the binding of CHMP1B(181-199) ( Table 2). Thus, the CHMP1B MIM1 motif appears to bind in the helix 2/3 groove of LIP5 MIT1. The LIP5(MIT) 2 groove mutant M64A also uniquely reduced binding of all of the other ESCRT-III MIM1 elements tested, indicating that all of these MIM1 elements also bind in the helix 2/3 groove of LIP5 MIT1 (Table 2).
In a complementary mapping approach, we used 1 H-15 N HSQC-NMR spectroscopy to identify which 15 N-labeled LIP5(MIT) 2 backbone amide resonances were perturbed by CHMP1B(181-199) binding (Fig. 4, A-C). LIP5(MIT) 2 backbone amide resonances that shifted upon addition of CHMP1B(181-199) (Fig. 4D, red) mapped almost exclusively to the surface presented by helices 2 and 3 of the first LIP5(MIT) 2 MIT domain (green). Thus, the two mapping approaches agreed well and are consistent with a structurebased model for MIM1-like binding of CHMP1B(181-199) (Fig. 4D, left, gray helix). We therefore conclude that CHMP1B binds the helix 2/3 surface of the first MIT module of LIP5, likely via a MIM1-MIT interaction.
Novel CHMP5 Element Binds the Second LIP5 MIT Module-A previous study showed that LIP5 binds CHMP5 with unusually high affinity (50), suggesting that the interaction with this ESCRT-III family member may be unique. The minimal LIP5binding site was mapped to CHMP5 residues 149 -173 (50), and we therefore initially mapped and quantified LIP5 binding using a CHMP5 fragment that spanned this region (CHMP5 (139 -195)). These studies employed a fluorescence polarization assay in which CHMP5(139 -191) was covalently linked to an Oregon Green 488 dye, and LIP5 binding was quantified by monitoring increases in fluorescence polarization. Normalized binding isotherms for the full-length LIP5 protein (residues 1-307) and a series of C-terminal truncation mutants are shown in Fig. 5. In excellent agreement with a previous report (50)  Biosensor binding analyses were used to quantify LIP5(MIT) 2 binding to a series of different immobilized GST-CHMP5 constructs. In good agreement with the fluorescence polarization data, LIP5(MIT) 2 bound tightly to full-length GST-CHMP5, with an estimated dissociation constant of 100 Ϯ 2 pM in this assay (Fig. 6, A and B). All other CHMP5 constructs that contained residues 150 -195 bound with similar affinities (e.g. Fig. 6C and summarized in Fig. 6A),  whereas the control N-terminal CHMP5(1-120) fragment did not bind. These data indicate that the LIP5(MIT) 2 -binding site spans predicted CHMP5 helices H5 and H6 and that LIP5 binding is not autoinhibited by the upstream core domain of CHMP5, which again agrees well with a previous study (50).
Structure of the LIP5(MIT)  Complex-To define the structural basis for the unusual LIP5-CHMP5 interaction, we determined the solution structure of the LIP5(MIT) 2 -CHMP5(139 -195) complex ( Fig. 7 and Table 1). As expected from the tight-binding affinity, the complex was in slow exchange on the NMR time scale, and the spectra were of high quality, particularly for a complex of 26 kDa (Fig. 8 and Table 1). As illustrated in Fig. 7A, the LIP5(MIT) 2 backbone structure did not change significantly upon CHMP5 binding. The LIP5(MIT) 2 -binding site comprises CHMP5 helices 5 and 6 and the linkers that flank either side of helix 5 (residues 155-189), which bind perpendicular to the helical bundle and wrap around three sides of LIP5 MIT2 (Fig. 7, B and C). These elements all make extensive contacts with LIP5 MIT2 and are well defined in the structure (Fig. 8). The N-terminal end of CHMP5 helix 5 crosses the MIT2 helix 6/7 groove, which would occlude binding of other ligands in this groove (e.g. MIM1 and MIM4 elements). The C-terminal end of CHMP5 helix 5 and the helix 5/6 linker sit in the groove between the two LIP5 MIT domains and make limited contacts with MIT1 helix 1 (Fig. 7C). CHMP5 helix 4 was also present and adopted a helical conformation but did not contact the MIT domain and appears to adopt many different orientations in solution ( Fig. 8 and legend).
The amphipathic CHMP5 helices 5 and 6 and linkers create a hydrophobic binding collar that includes a series of six leucine residues, and we therefore term this motif the "leucine collar" (Fig. 7B). All six leucine residues are invariant across mammals, and conservative substitutions occur only in the first leucine in other metazoan species (Fig. 2B) Fig. 7 for a list of intermolecular salt bridges and hydrogen bonds). Thus, the unusually high affinity of the LIP5-CHMP5 interaction appears to result from a combination of extensive hydrophobic interactions made by the extended leucine collar, together with charge complementarity between the basic LIP5 MIT2 module and the highly acidic CHMP5 binding site. The interaction buries a total surface area of 2540 Å 2 , which is substantially greater than any previously characterized MIM-MIT interaction and which is consistent with the unusually high binding affinity.
As noted above, LIP5 constructs that terminated in the vicinity of residue 170 exhibited somewhat tighter CHMP5 binding, and we therefore considered the possibility that the LIP5(1-168)-CHMP5(139 -195) complex might have an unusual or aberrant structure. To test this possibility, we compared the 1 H-15 N HSQC-NMR spectrum of the higher affinity 15 N-labeled LIP5(1-168)-CHMP5(139 -195) complex with that of the lower affinity 15 N-labeled LIP5(1-183)-CHMP5(139 -195) complex. The two spectra were superimposable, with no significant backbone amide chemical shift changes except at the extreme termini, where the two LIP5(MIT) 2 constructs differ in length and sequence (data not shown). We therefore concluded that all of the different LIP5(MIT) 2 -CHMP5 complexes under study likely have very similar structures. We do not have a definitive explanation for the underlying cause of the higher binding affinities seen for LIP5 constructs that terminate near residue 170, but we speculate that it may reflect a favorable charge interaction between the (non-native) LIP5 C termini and the neighboring CHMP5 Arg-151 residue.

LIP5-Ligand Interactions
quadruple mutation reduced CHMP5(139 -195) binding to undetectable levels (Fig. 6, A and E). The LIP5(MIT) 2 -CHMP5(139 -195) structure also suggested that Trp-147 was likely to play a key role on the LIP5 side of the interface. We found that a W147D mutation in LIP5(MIT) 2 reduced CHMP5(139 -195) binding by 3000-fold in the biosensor binding assay (Fig. 6, A and D), and the detrimental effect was even greater in the fluorescence polarization binding assay (data not shown). Thus, our mutational analyses supported the validity of the LIP5(MIT) 2 -CHMP5(139 -195) structure and identified non-binding mutants that could be used in cell-based studies.
Lack of Cooperativity or Autoinhibition in LIP5-Ligand Interactions-Although many components of the ESCRT pathway engage in autoinhibitory and/or cooperative binding interactions, the structure of the LIP5(MIT) 2 -CHMP5 complex did not reveal any conformational changes that appeared likely to influence LIP5 MIT1 binding to ESCRT-III MIM1 motifs. This lack of binding cooperativity was confirmed in biosensor binding experiments that showed that saturation of the MIT2 binding site with CHMP5(133-195) did not alter the binding affinity of CHMP1B(169 -199) for LIP5(MIT) 2 (Fig. 3B, compare closed diamonds and open squares; and Table 2). Thus, the two different types of ESCRT-III ligands bind the LIP5 MIT modules without positive or negative cooperativity.
It has been suggested that the terminal domains of Vta1p may interact and mutually autoinhibit their different ligand interactions (58), so we also tested for autoinhibition within LIP5 by comparing the binding affinities of different ligands for full-length LIP5 versus the isolated LIP5(MIT) 2 and LIP5(VSL) domains. Our binding assays were configured to measure differences in intrinsic ligand binding affinities rather than avidity effects. As discussed above, CHMP5(139 -195) bound equally tightly to full-length LIP5 and to a minimal LIP5(MIT) 2 construct (Figs. 5 and 6), implying that downstream LIP5 sequences did not autoinhibit CHMP5 binding to the LIP5 MIT2 module. Similarly, biosensor binding analyses showed

LIP5-Ligand Interactions
that CHMP1B(181-199) bound with the same affinity to LIP5 constructs that spanned the full-length protein (residues 1-307), the MIT2 domain, and the downstream linker (residues 1-259) or the MIT2 domain alone (residues 1-168) (Fig. 9A). These experiments indicate that MIM1 binding to the LIP5 MIT1 module is not autoinhibited by downstream LIP5 sequences.  Finally, NMR chemical shift titration analyses were used to quantify binding of the LIP5 constructs to VPS4B (Fig. 9, B-D). These binding studies utilized a construct that comprised the small ATPase and inserted ␤-domains of VPS4B (termed SAB for small ATPase and beta, residues 300 -423). As shown in Fig.  9 (C and D), binding of either full-length LIP5 or the LIP5(VSL) domain alone (residues 260 -307) induced very similar backbone amide chemical shift changes in the same subset of residues within 15 N-labeled VPS4B(SAB), indicating that both LIP5 constructs bound in a very similar fashion. Chemical shift titration data for three shifted residues (designated A, B, and C) were independently fit to single-site binding models in each complex, yielding similar equilibrium dissociation constants for VPS4B(SAB) binding to either full-length LIP5 (K D ϭ 170 Ϯ 30 M) or the VSL domain alone (K D ϭ 233 Ϯ 1 M) (Fig. 9B). Thus, our data indicate that external LIP5 elements do not autoinhibit ligand binding to any of the three known protein interaction sites within the LIP5(MIT) 2 and LIP5(VSL) domains.
Interdependence of LIP5-Ligand Binding in Vivo-Co-immunoprecipitation experiments were used to test the relevance of The left view shows that CHMP5 residues 155-189 (red) are well ordered, and the right view shows that LIP5 residues 1-163 are well ordered. Disordered residues at the protein termini (gray) were omitted from the structures shown in Fig. 7 and from the statistics reported in Table 1. We concluded that CHMP5 helix 4 is not involved in LIP5(MIT) 2 binding because 1) there were no intermolecular NOEs between CHMP5 helix 4 and LIP5(MIT) 2 ; 2) residual dipolar coupling values for CHMP5 helix 4 were small, indicating that this helix might not have a unique orientation (unlike helices 5 and 6); 3) resonances within this helix were sharper and relaxed more slowly than those in the rest of the protein; and 4) deleting this helix did not affect LIP5 binding affinity (Fig. 6A,   . Shifted resonances are labeled as described for C. Note that the chemical shifts induced by binding of full-length LIP5 and LIP5(VSL) alone are very similar. Our estimated dissociation constant for the human LIP5(VSL)-VPS4B(SAB) interaction (ϳ200 M) is considerably weaker than a previous estimate of the K D for full-length LIP5 binding to immobilized VPS4B/SKD1 (53 nM), but that measurement was made under conditions in which avidity effects could have contributed to the observed K D because the dimeric LIP5 protein was binding immobilized VPS4B/SKD1 (46). Our dissociation constant is ϳ3-fold weaker than that reported previously for the yeast Vta1p(VSL)-Vps4p(SAB) interaction (48), and this agreement seems reasonable given that the proteins are from different species and that the previous assay was also configured so that avidity effects could have contributed to the measured binding affinity. ligand interactions was confirmed and extended by experiments that demonstrated the following. 1) Endogenous CHMP5 and VPS4A proteins co-immunoprecipitated with Myc-LIP5 (Fig. 10B, first lane), and both of these interactions were inhibited by a LIP5 point mutation that inhibited CHMP5 binding (W147D mutation in LIP5 MIT2) (second lane). 2) VPS4A and endogenous LIP5 proteins both co-immunoprecipitated with CHMP5-Myc (Fig. 10C, first lane), and both of these interactions were inhibited by a CHMP5 mutation that blocked LIP5 binding (L163D,L167D,L170D,L174D mutation (denoted L4D) in the CHMP5 binding motif) (compare the second lane). 3) siRNA depletion of CHMP5 inhibited VPS4 binding to LIP5 (Fig. 10D, compare the first and second lanes). 4) Point mutations on either side of the LIP5-CHMP5 interface inhibited VPS4A-EGFP co-immunoprecipitation of both CHMP5-Myc and Myc-LIP5 (Fig. 10E, compare the second and third lanes with the first lane). Taken together, these experiments demonstrated that VPS4A forms stable precipitable complexes with both LIP5 and CHMP5 in cells and that these complexes form only if LIP5 can interact with both CHMP5 and CHMP1B (and/or other MIM1-containing ESCRT-III proteins).

DISCUSSION
Like many other cellular machines that must function transiently, the ESCRT pathway is tightly regulated to ensure that the machinery localizes to correct sites of action, forms properly, and assembles and disassembles with "all or none" behavior. ESCRT regulation occurs at a number of different steps and via a number of different mechanisms, including the use of membrane-specific adaptor proteins, ordered protein-protein and protein-membrane interactions, post-translational modifications such as ubiquitylation and phosphorylation, autoinhibition, allostery, and avidity (1)(2)(3)(4)(5)(6)(7)(8)(9). VPS4 activation is one of the highly regulated steps in the ESCRT pathway and is governed primarily by the actions of the activator protein LIP5. LIP5 comprises two well ordered domains: a tandem MIT domain at the N terminus and a VSL dimerization domain at the C terminus, separated by a disordered linker region of ϳ100 residues ( Fig. 1) (58). Our analyses have helped to reveal how LIP5 connects VPS4 to the ESCRT-III lattice by demonstrating that the tandem LIP5 MIT domain must make two different types of interactions with CHMP5 and CHMP1B (and/or other ESCRT-III proteins) in order for LIP5 and CHMP5 to form precipitable cellular complexes with VPS4. We did not find any evidence that the VSL and MIT domains of LIP5 communicate directly (e.g. Figs. 5 and 9). Rather, our data support a model in which multiple weak ligand interactions, together with avidity effects resulting from LIP5 dimerization, cooperate to recruit LIP5 to oligomeric ESCRT-III assemblies and activate VPS4 at the proper time and place.
CHMP1B Interactions with LIP5(MIT) 2 -Our analyses agree well with previous studies that mapped LIP5/Vta1p binding to the C terminus of CHMP1B/Did2p (Fig. 3) (34, 50). However, a previous study mapped the interaction of yeast Did2p to the second MIT module of Vta1p (58), whereas we found that CHMP1B binds the first MIT module of human LIP5. Our studies therefore provide functions for both LIP5 MIT modules because CHMP1B and other ESCRT-III MIM1 elements bind the first MIT module and CHMP5 binds the second MIT module. Our mutational and NMR spectroscopic data are consistent with a model in which the terminal helix of CHMP1B makes a MIM1-like interaction with the helix 2/3 groove of the first LIP5 MIT module. CHMP1B/Did2p is an unusual ESCRT-III protein in that it binds well to the MIT domains from both VPS4 and LIP5 (33,34,39,40,50,58). This is apparently possible because the helix 2/3 grooves of the MIT domains from VPS4 and LIP5 are similar in character. In particular, LIP5 MIT1 residues that comprise the predicted MIM1-binding groove (Leu-40, Met-43, Met-47, Leu-60, Met-64, and Leu-67) retain the hydrophobic character and are generally similar to the corresponding residues within the analogous VPS4A MIT groove (Gln-32, Val-36, Leu-40, Cys-60, Leu-64, and Arg-67). Our structure-based alignments also predict that an important intermolecular salt bridge formed in the VPS4A-CHMP1A complex (VPS4A MIT Asp-65/Glu-68 -CHMP1A Arg-121) (35,36) is conserved in the LIP5-CHMP1B complex (as LIP5 Asp-65/Glu-68 -CHMP1A Arg-121). Conversely, the chemical character of the MIM1-binding site on the second LIP5 MIT module (the helix 6/7 groove) is significantly more polar in character, with charged residues at Asp-126, Arg-142, and Arg-146, and this likely explains why CHMP1B MIM1 does not bind in this groove.  DECEMBER 21, 2012 • VOLUME 287 • NUMBER 52 JOURNAL OF BIOLOGICAL CHEMISTRY 43923 LIP5(MIT) 2 failed to bind MIM2 elements from CHMP4B, CHMP6, or IST1 or to bind the MIM3 element from CHMP1B, all of which should bind in MIT helix 1/3 grooves. The helix 1/3 groove of the first MIT domain of LIP5(MIT) 2 is filled by the N-terminal strand of LIP5, which will occlude MIM2 and MIM3 binding unless this strand is displaced. It is less clear why MIM2 or MIM3 elements did not bind the equivalent helix 5/7 groove of the second LIP5 MIT domain, but there are several substitutions versus the VPS4A MIT helix 1/3 groove that could reduce MIM2 binding affinities. Alternatively, the terminal type I turn of LIP5(MIT) 2 orients the mobile LIP5 residues 164 -170 along this groove, and this may sterically inhibit ligand binding. In summary, although the tandem MIT domain of LIP5 has four different grooves that could, in principle, engage up to four different MIM elements, we found evidence only for MIM1 element binding in the helix 2/3 groove of the first MIT module.

LIP5-Ligand Interactions
CHMP5 Binding to LIP5(MIT) 2 -Our biochemical and structural studies demonstrated that CHMP5 helices 5 and 6 and adjacent linkers form an amphipathic leucine collar that wraps almost completely around the second MIT module of LIP5(MIT) 2 . This is the first example in which an ESCRT-III MIM element binds perpendicular to and outside of the MIT grooves (designated a MIM5-MIT interaction) (Fig. 11). The interaction is also the most extensive MIM-MIT interaction seen to date in terms of both buried surface area and binding affinity (Figs. 6 and 7). Interactions with the first LIP5 MIT module are minimal, however, and CHMP5 binding does not alter the affinity of CHMP1B binding to MIT1 (Fig. 3B). The binding of CHMP5 would clearly occlude the binding of other MIM elements in the helix 6/7 groove but does not obviously alter the accessibility of the helix 5/7 groove, which could therefore, in principle, bind an additional unidentified ligand(s).
A previous study mapped the yeast Vps60p/CHMP5 interaction to the second MIT module of Vta1p/LIP5 (58). Nevertheless, there again appear to be significant differences between the analogous metazoan and yeast interactions because the primary Vta1p interaction site was mapped to Vps60p helix 4 (34), whereas the LIP5 interaction site spans CHMP5 helices 5 and 6, and CHMP5 helix 4 does not contact LIP5(MIT) 2 (Figs. 6 -8) (53). In most ESCRT-III proteins, the fifth helix and surrounding regions can fold back onto the core domain and make autoinhibitory interactions (12,53,75,76). We found, however, that LIP5(MIT) 2 bound with the same affinity to full-length CHMP5 as to the minimal CHMP5 binding site (Fig. 6A), implying either that CHMP5 does not exhibit autoinhibition or that the LIP5binding site on CHMP5 is exposed in the "closed" CHMP5 conformation (53).
LIP5-Ligand Interactions in Cells-We also performed a series of co-immunoprecipitation experiments to examine the interdependence of different LIP5-ligand interactions within cells. Our data support the relevance of our LIP5-CHMP5 structural studies because structure-based mutations on either side of the LIP5-CHMP5 interface inhibited complex formation both in vitro (Fig. 6) and in cells (Fig. 10). Furthermore, our data indicate that both of the LIP5(MIT) 2 interactions that we characterized are required to make stable precipitable cellular VPS4 complexes with both LIP5 and CHMP5. Specifically, mutations or siRNA depletions that disrupted the LIP5-CHMP5 interaction blocked VPS4A co-immunoprecipitation with both proteins. Similarly, a mutation in the first MIT domain of LIP5 that inhibited the binding of CHMP1B (and other MIM1-containing ESCRT-III proteins) also inhibited coprecipitation of VPS4A with LIP5 (Fig. 10A). In the absence of detailed localization and functional studies, we can only speculate about the nature of these VPS4 complexes and where in the cell they form. Nevertheless, our data are consistent with published models in which LIP5 and CHMP5 are initially tightly associated in the cytoplasm (53). Formation of stable ESCRT-III-associated LIP5-VPS4 complexes might then require both a direct LIP5(VSL)-VPS4(SAB) interaction and a supporting interaction between the CHMP1B MIM1 element and the first LIP5 MIT domain (33,45). It is not yet clear why CHMP5 is also required to form this complex, but it was recently shown that CHMP5 can bind the ALIX paralog BROX at a site located just downstream of the LIP5-binding site (77). BROX and ALIX both contain Bro1 domains, and the two proteins can function interchangeably in initiating some ESCRT assembly reactions (78). We therefore speculate that the BROX-CHMP5 interaction might also contribute to targeting the LIP5-CHMP5 complex to membrane-associated ESCRT-III assemblies (although it is alternatively possible that CHMP5 helps target BROX to these sites). Once recruited, LIP5 presumably promotes VPS4 assembly and activates the enzyme to remodel the ESCRT-III polymer and release ESCRT factors back into the cytoplasm.