Nitric Oxide Modulates Sodium Vitamin C Transporter 2 (SVCT-2) Protein Expression via Protein Kinase G (PKG) and Nuclear Factor-κB (NF-κB)*

Background: Ascorbate is an important antioxidant that is carried into and out of cells by its high-affinity transporter sodium vitamin C transporter-2 (SVCT-2). Results: Nitric oxide increases SVCT-2 protein levels and ascorbate uptake. Conclusion: Nitric oxide exerts a fine-tuned control of ascorbate availability to retinal cells. Significance: Neuroprotective role of nitric oxide may be achieved by regulating SVCT-2 expression. Ascorbate is an important antioxidant, which also displays important functions in neuronal tissues, including the retina. The retina is responsible for the initial steps of visual processing, which is further refined in cerebral high-order centers. The retina is also a prototypical model for studying physiologic aspects of cells that comprise the nervous system. Of major importance also is the cellular messenger nitric oxide (NO). Previous studies have demonstrated the significance of NO for both survival and proliferation of cultured embryonic retinal cells. Cultured retinal cells express a high-affinity ascorbate transporter, and the release of ascorbate is delicately regulated by ionotropic glutamate receptors. Therefore, we proposed whether there is interplay between the ascorbate transport system and NO signaling pathway in retinal cells. Here we show compelling evidence that ascorbate uptake is tightly controlled by NO and its downstream signaling pathway in culture. NO also modulates the expression of SVCT-2, an effect mediated by cGMP and PKG. Kinetic studies suggest that NO increases the transport capacity for ascorbate, but not the affinity of SVCT-2 for its substrate. Interestingly, NO utilizes the NF-κB pathway, in a PKG-dependent manner, to modulate both SVCT-2 expression and ascorbate uptake. These results demonstrate that NO exerts a fine-tuned control of the availability of ascorbate to cultured retinal cells and strongly reinforces ascorbate as an important bioactive molecule in neuronal tissues.

Ascorbate is an important antioxidant, which also displays important functions in neuronal tissues, including the retina. The retina is responsible for the initial steps of visual processing, which is further refined in cerebral high-order centers. The retina is also a prototypical model for studying physiologic aspects of cells that comprise the nervous system. Of major importance also is the cellular messenger nitric oxide (NO). Previous studies have demonstrated the significance of NO for both survival and proliferation of cultured embryonic retinal cells. Cultured retinal cells express a high-affinity ascorbate transporter, and the release of ascorbate is delicately regulated by ionotropic glutamate receptors. Therefore, we proposed whether there is interplay between the ascorbate transport system and NO signaling pathway in retinal cells. Here we show compelling evidence that ascorbate uptake is tightly controlled by NO and its downstream signaling pathway in culture. NO also modulates the expression of SVCT-2, an effect mediated by cGMP and PKG. Kinetic studies suggest that NO increases the transport capacity for ascorbate, but not the affinity of SVCT-2 for its substrate. Interestingly, NO utilizes the NF-B pathway, in a PKG-dependent manner, to modulate both SVCT-2 expression and ascorbate uptake. These results demonstrate that NO exerts a fine-tuned control of the availability of ascorbate to cultured retinal cells and strongly reinforces ascorbate as an important bioactive molecule in neuronal tissues.
Ascorbate is a six carbon-composed sugar that displays many functions in neuronal cells, such as maturation of the glutama-tergic system in cultured hippocampal cells (1) and modulation of NMDA receptor function (2). Vitamin C is a widespread term that defines its oxidized (dehydroascorbate) and its reduced forms (ascorbate). Ascorbate is transported by a highaffinity protein, named sodium vitamin C transporter (SVCT) 3 (3)(4)(5), which transports ascorbate in a sodium-dependent manner, using two Na ϩ ions for each ascorbate molecule transported (3,5). There are two human cloned isoforms of SVCT (SVCT-1 and -2), which are encoded by the genes SLC23A1 and SLC23A2, respectively (6,7). The two isoforms display a high percentage homology in their primary transcripts and serve different functions among distinct tissues (6). Consensus motifs for phosphorylation by PKA and PKC have also been described within the SVCT (3)(4)(5). In the nervous system, ascorbate contributes to the formation of the myelin shaft (8,9) and negatively modulates Na ϩ /K ϩ -ATPase activity (10,11). Ascorbate has also been reported to be present in chicken embryo eyes since early stages of development (12). Previous studies have demonstrated the presence of SVCT-2 transcripts in the inner nuclear layer of the rat retina (3). Moreover, presence of the SVCT-2 protein has been described in the inner nuclear and ganglion cell layers of the chicken retina (13). In cultured chicken retinal cells it has been demonstrated that ascorbate release is modulated by ionotropic glutamate receptors and proposed that ion flux through AMPA/kainate receptors promotes SVCT-2 reversal (13).

* This work was supported in part by grants from Conselho Nacional de
Desenvolvimento Científico e Tecnoló gico, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Fundação de Amparo à Pesquisa de Estado do Rio de Janeiro, Programa de Apoio de Nú cleos de Excelê ncia/ Ministé rio da Ciê ncia e Tecnologia, and Projeto Estraté gico C/SAU/UI3282/ 2011 (FCT, Portugal, and FEDER-COMPETE-POFC). 1 Recipients of student fellowships from CAPES and CNPq. 2  The gaseous signaling molecule NO is produced from the amino acid L-arginine in a reaction catalyzed by nitric-oxide synthase (NOS) enzymes (14). The reaction also requires molecular oxygen with the concomitant production of NO and L-citrulline (15). NO can mediate its effects by two mechanisms: first, the classical activation of soluble guanylyl cyclase (sGC, also defined as a NO-receptor) with production of cGMP and activation of PKG, known as the sGC/cGMP/PKG pathway (16 -19); second, NO can covalently attach to cysteine residues within the amino acid sequence of a specific sets of proteins, a mechanism known as S-nitrosylation (19,20). Hitherto, the involvement of NO and its related downstream signaling pathways in several developmental aspects of the CNS has been demonstrated, for instance, neuronal proliferation, survival, and differentiation (21)(22)(23)(24)(25). Previous studies have demonstrated the anti-proliferative role of NO in the developing chick retina (25) and its neuroprotective role upon cultured retinal cells in relationship to culture-refeeding stress (21). Moreover, a high-affinity transport system for L-arginine as well as the neuronal NOS have been shown to be expressed in cultured retinal neurons (26). NO has also been shown to modulate the phosphorylation of ERK MAP kinase and cAMP responsive element-binding protein (CREB) transcription factor in cultured retinal cells (27).
The NF-B is a dimeric transcription factor involved in cell survival and differentiation (28). It modulates a large number of genes in response to infection, inflammation, and other stressful situations that require rapid reprogramming of gene expression (29). When NF-B is attached to IB (NF-B inhibitor), it remains in its inactive state at the cytoplasm (28). The IB kinase (IKK) is a regulatory complex composed of two catalytic subunits (IKK␣/1 and IKK␤/2) and a structural subunit known as NEMO/IKK␥ (30). When active, this complex phosphorylates IB, which is degraded by the ubiquitin system (29,31,32). As a result, free NF-B can be translocated to the nucleus and modulates gene transcription. NF-B is also a redox-sensitive transcription factor and has already been shown to modulate SVCT2 mRNA expression in response to redox-state unsteadiness in C2C12 myotubes (7). Furthermore, it has already been suggested that LPS-modulated iNOS activity may regulate NF-B activation in non-neuronal cells (33). Moreover, the control of NO production in inflammatory processes, such as LPS-induced macrophage activation, is achieved by transcriptional regulation of the iNOS gene by the NF-B pathway (34).
As ascorbate and NO seem to play critical roles in neuronal cells, we are interested in studying the effects of NO and its related signaling pathways on the ascorbate uptake in cultured retinal cells. Here we show for the first time that NO strictly modulates SVCT-2 expression and ascorbate uptake in retinal cells in culture, an effect mediated by the sGC/cGMP/PKG signaling pathway and NF-B system.
Mixed Primary Culture-Eight-day-old chicken embryo retinas (E8) were dissected from other ocular tissues (including from the retinal pigmented epithelium) and digested with 0.1% trypsin (w/v) in Ca 2ϩ and Mg 2ϩ -free Hanks' balanced salt solution (HBSS) for 12 min at 37°C. Then, cells were suspended in minimum essential medium, supplemented with 3% FBS (v/v), 100 units/ml of penicillin, 100 g/ml of streptomycin and glutamine (2 mM), dissociated using a Pasteur pipette, and seeded in 40-mm or 24-well culture plastic dishes in a density of 2 ϫ 10 7 cells/mm 2 . Cells were maintained at 37°C in a humidified incubator with 5% CO 2 , 95% air (v/v) for 3 to 4 days. The presence of neurons and glial cells in these cultures has been previously characterized (35).
Purified Neurons Primary Culture-Purified neurons were cultured as previously described (36). Briefly, retinas from 8-day-old chick embryos were dissected free from other ocular tissues (including the retinal pigmented epithelium), incubated with 0.1% trypsin (w/v) for 8 min, and then dissociated using a fire-polished Pasteur pipette. The cells were plated on poly-Lornithine-coated dishes at a density of 10 4 cells/mm 2 in medium 199 containing 10% FBS (v/v), 100 units/ml of penicillin, 100 g/ml of streptomycin and glutamine (2 mM). Cultures were kept in a humidified atmosphere containing 5% CO 2 , 95% air (v/v) at 37°C for 3 days.
[ 14 C]Vitamin C Uptake-E8C3 cultures were rinsed twice with HBSS, containing NaCl 140 mM; KCl 5 mM; Hepes 20 mM; glucose 4 mM; MgCl 2 1 mM; and CaCl 2 2 mM, pH 7.4. For uptake experiments, cultured cells were preincubated for 10 min at 37°C with HBSS in the absence or presence of different drugs, incubated with [ 14 C]ascorbate (0.1 Ci/ml) during the indicated periods, and lysed with 5% TCA (w/v) for determination of intracellular radioactivity.
Kinetic Analysis-Mixed cultured primary cells were washed twice with HBSS and preincubated or not with the indicated stimuli (100 M SNAP or 2 mM L-arginine) for 10 min. Cultures were then incubated with increasing concentrations (0, 1, 2, 5, 10, 15, 25, 50, 100, 200, and 500 M) of ascorbate, [ 14 C]ascorbate (0.1 Ci/ml), and in the presence or absence of stimuli for 5 min. The reaction was stopped by 5% TCA (w/v), the radioactivity was analyzed by liquid scintillation and the total amount of protein was determined by the Lowry method. Ascorbate content in each time point was calculated using its specific activity per protein content per minute. Data were first set up for a two-site (specific biding) nonlinear regression according to the equation: Highand low-affinity components were plotted using the Eadie-Hofstee transformation. Data were analyzed using GraphPad Prism version 5.00 for Windows (GraphPad Software, San Diego, CA).
Nuclear Fraction Preparations-E8C3 mixed cultures were washed twice with HBSS and treated as specified under "Results." Next, saline was removed and cells were lysed with lysis buffer (50 mM Tris-HCl, 5 mM EDTA, 100 mM NaCl, 1 mM DTT, 100 M PMSF, 200 M sodium orthovanadate, 1% Triton X-100, 4 g/ml of leupeptin). The material was scrapped off the dishes and transferred to tubes, which were kept in constant motion at 4°C for 10 min. Then, the tubes were centrifuged (16,000 ϫ g for 10 min at 4°C), the supernatant (cytoplasmic fraction) was collected, and the pellet (nuclear fraction) was re-suspended. Both fractions were next subjected to electrophoresis and Western blotting. To confirm the efficacy of the separation assay we used fractional markers: cytochrome c (cytoplasm fraction) and histone H 2AX (nuclear fraction).
Total RNA Extraction, cDNA Synthesis, and Quantitative Real Time PCR-Harvested cells from mixed retinal cultures were resuspended in 1 ml of TRIzol reagent. Total RNA was extracted according to the manufacturer's instructions, and its quality and concentration were determined using a Nano-Drop ND-1000 Spectrophotometer (Thermo Scientific, Wilmington, DE). First strand synthesis was performed using 2 g of total RNA (DNase I treated), random primers, and the SuperScript First-Strand Synthesis System for RT-PCR kit. qRT-PCR was carried out using iTaq SYBR Green Mastermix with ROX (Bio-Rad) on an iQ TM 5 multicolor realtime PCR detection system (Bio-Rad). The final reaction volume was 20 l. Reaction conditions were: denaturation at 95°C for 3 min and 48 cycles of 95°C for 15 s and annealing temperature (T m ) for 30 s. Melt curve analysis was performed for quality control at the end of each reaction. Primers were designed using Primer3plus: Slc23a2 forward, TC-TTCCAGCCCTAACATTGG and reverse, ACTGAACTT-GCCAACCATCC; Gapdh forward, GACCCCAGCAACAT-CAAATG and reverse, TTAGCACCACCCTTCAGATG and rpL27 forward, CATTCCCCTGGACAAAACTG and reverse, ATCGCAGCTTCTGGAAGAAC. Primer concentration, T m , and reaction efficiency (E) were optimized for each primer set (Slc23a2, 200 nM, 58°C, 1.94; Gapdh, 100 nM, 58°C, 1.91; rpL27, 300 nM, 60°C, 2.00). The efficiency was analyzed using a log-based standard curve (with cDNA pool from all samples) for each gene, according to the equation: E ϭ 10 (Ϫ1/slope) . Relative quantities of Slc23a2 products in control versus SNAP-treated cultures were calculated by the method described by Pfaffl (37) using GAPDH and L27 as reference genes.
Intact Retina Experiments-E8 embryos had their retina dissected at 4°C and were equilibrated in HBSS for 20 min at 37°C. Next, uptake experiments were carried out as described above. The assay was stopped by adding 5% TCA (w/v) and the material was frozen overnight. The material was centrifuged at 20,627 ϫ g for 15 min at 4°C. The supernatant was collected and the radioactivity measured by liquid scintillation. The pellet was re-suspended in 1 M NaOH and the protein content in each sample was determined by the Lowry method.

SVCT-2 Regulation by Nitric Oxide
Statistical Analysis-The data were evaluated by one-way analysis of variance followed by the Bonferroni post-test and unpaired Student t test, using GraphPad Prism software.

RESULTS
Model Characterization-With the aim of better characterizing our model system, we performed immunocytochemistry in combination with confocal microscopy to define the neuron/ glia proportion in these cultures. We observed that 80.7% of labeled cells were TUJ ϩ and 19.3% were 2M6 ϩ cells (Fig. 1A). Moreover, in accordance with our goal, we evaluated the expression of NOS isoforms in our model system. The presence of nNOS in neurons has already been described, but not in glial cells, in this culture paradigm (26); so we analyzed the presence of the two other NOS isoforms. As seen in Fig. 1B, iNOS immunostaining in control cells is completely absent. On the other hand, eNOS positive cells were present in these cultures (Fig.  1B). To determine in which cell type eNOS is expressed, we performed double-labeling experiments with TUJ and 2M6, specific markers for neuronal and glial cell populations, respectively. Fig. 1C demonstrates that eNOS is co-localized with both TUJ and 2M6 positive cells.
NO Enhances Ascorbate Uptake in a sGC/cGMP/PKG-dependent Manner-Previous works have clearly suggested that NO is an important signaling molecule in retinal cell cultures. Therefore, we tested whether NO modulates ascorbate uptake in cultured retinal cells. SNAP, a NO donor, was capable of increasing ascorbate uptake in a dose-dependent manner, with low doses (e.g. 1 and 10 M) displaying significant effects in the uptake of ascorbate ( Fig. 2A). As SNAP is a nitrosothiol compound, we further confirmed whether its effect was mediated by releasing NO. As can been seen in Fig. 2B, SNAP-induced ascorbate uptake was mediated by the release of NO because it was abolished by cPTIO (100 M), a NO scavenger (from 62.6 Ϯ 5.6% to Ϫ4 Ϯ 15.3%).
To correlate endogenous levels of NO with ascorbate uptake, a dose-response curve of L-arginine (the endogenous substrate of NOS) was carried out and a dose-dependent increase in ascorbate uptake was observed (Fig. 2C). Moreover, the L-arginine effect was completely abolished when cultures were treated with 100 M 7-NI (from 59 Ϯ 9.3% to Ϫ11 Ϯ 0.3%; Fig.  2D) or 100 M L-NAME (from 59 Ϯ 9.3% to 2.9 Ϯ 0.2%; Fig. 2D), both selective inhibitors of NOS.

SVCT-2 Regulation by Nitric Oxide
NO Modulates the Kinetics of Ascorbate Uptake-As stated above, we have observed the involvement of the classical NO signaling pathway in ascorbate uptake; therefore, we performed kinetic assays in the presence of either L-arginine or SNAP to clarify the mechanism involved in the NO-induced increase of ascorbate uptake (Fig. 3). The Eadie-Hofstee plots (Fig. 3, B-D) demonstrated that the concentration curve of ascorbate uptake displayed two components: one with high-affinity and another with low-affinity. Interestingly, both L-arginine (Fig. 3C) and SNAP (Fig. 3D) treatment increased the V max of both the lowand high-affinity components, respectively, without modifying their K m . Therefore, we suggest that NO modulates the transport capacity of SVCT-2.
NO Increases SVCT-2 Expression and Modulates Ascorbate Uptake through Its Canonical Pathway-As observed, NO increases the transport capacity for ascorbate in cultured retinal cells. We then tested whether this effect could be promoted by increased SVCT-2 expression by evaluating the effect of NO on the expression levels of SVCT-2. As observed in Fig. 4, A and  B, NO was capable of increasing SVCT-2 protein levels in a time-dependent manner in cultured retinal cells. Moreover, immunocytochemistry labeling corroborates Western blotting data showing that SNAP strongly enhances expression of the SVCT-2 protein in cultured retinal cells (Fig. 4, C and D). To extend these data, actinomycin D (1 g/ml), an inhibitor of transcription, completely abolished the SNAP-induced increase in SVCT-2 expression (Ϫ8.8 Ϯ 24.1%, n ϭ 5; Fig. 5A). To validate that SNAP indeed regulated SVCT-2 mRNA transcription, qRT-PCR was performed and SNAP promoted an ϳ1.8-fold increase in the slc23a2 gene product (Fig. 5B), demonstrating that NO regulates SVCT-2 expression at the transcriptional level. To ratify that NO modulated SVCT-2 also at the translational level, anisomycin, a protein synthesis inhibitor, completely blocked the NO-induced SVCT-2 protein increase (Ϫ19.7 Ϯ 33.4, n ϭ 3; Fig.  5C). In addition, CHX, another inhibitor of protein synthesis, also blocked the SNAP effect (data not shown). To confirm that NO was modulating ascorbate uptake due to boosting SVCT-2 expression levels, we used actinomycin D, CHX, and anisomycin in ascorbate uptake assays (Fig. 5, D and E). SNAP-induced increase in ascorbate uptake was completely blocked by actinomycin D (Ϫ11.7 Ϯ 13.6%, n ϭ 3; Fig. 5D), CHX (Ϫ3.8 Ϯ 1.1%, n ϭ 3; Fig. 5E), or anisomycin (1.3 Ϯ 7.5%, n ϭ 3; Fig. 5E). Therefore, it seems that NO increases SVCT-2 protein expression and as a result modulates ascorbate uptake.
We also evaluated the involvement of the canonical NO signaling pathway in modulating SVCT-2 expression. As occurs
NO-stimulated Increase in SVCT-2 Expression Is Mediated by NF-B-It has been shown that NF-B can regulate SVCT-2 expression (7) and we decided to investigate whether SNAP-induced modulation in SVCT-2 expression could be mediated by NF-B. First, we evaluated, in our model, whether NO was capable of modulating NF-B expression. We observed that NO increased NF-B expression (Fig. 7, A-C). Moreover, using cellular fraction-ation assays we showed that, when cultured retinal cells were treated with SNAP or L-arginine, there was an increase in both IKK and IB phosphorylation at the cytoplasm compartment (Fig. 7D). Additionally, these treatments also induced the translocation of NF-B from the cytoplasm to the nuclear compartment (Fig. 7E). Therefore, it seems that NO regulates NF-B activation in cultured retinal cells.
Next, we evaluated the relationship between NF-B and the modulatory effect played by NO in both SVCT-2 expression and ascorbate uptake. As observed in Fig. 8, A-C, both L-arginine and a SNAP-induced increase in SVCT-2 expression are regulated by NF-B, because it was blocked by the inhibitors of the NF-B pathway, PDTC (100 M) or sulfasalazine (100 M). Moreover, either L-arginine or SNAPinduced ascorbate uptake was abolished by PDTC or sul-  FEBRUARY 3, 2012 • VOLUME 287 • NUMBER 6 fasalazine (Fig. 8, D and E), indicating that the NF-B signaling pathway controls the expression of SVCT-2 and, as a result, the uptake of ascorbate in cultured retinal cells.

SVCT-2 Regulation by Nitric Oxide
NO Modulates Ascorbate Uptake and SVCT-2 Expression in the Intact Retina-To evaluate the role played by NO in a more physiological fashion we have made use of the intact retinal tissue. This paradigm is interesting because both the cytoarchitecture of retinal layers and the cellular interactions between retinal cell types are preserved within the developing retina, unlike the employed retinal cell culture system. Interestingly, L-arginine and SNAP increased both SVCT-2 expression (Fig.  9A) and ascorbate uptake (Fig. 9B) in the intact retina, suggesting that the interplay between NO and the ascorbate system may contribute to neurochemical aspects in retinal physiology.

DISCUSSION
In this work we have shown that NO increased ascorbate uptake and SVCT-2 expression through its canonical signaling pathway in cultured retinal cells. NO also regulated the NF-B transcription factor activation in this paradigm (measured by enhanced pIB/pIKK phosphorylation and consequent NF-B translocation to the cell nucleus). This process is ultimately related to the NO-modulated ascorbate uptake (Fig. 8). Moreover, NO also promoted the increase of ascorbate uptake and SVCT-2 expression in a more physiological and reliable system, the intact retinal tissue.
Cross-talk between NO and Ascorbate-We have demonstrated here that NO induced an increase of SVCT-2 protein levels. When each step of the canonical NO signaling pathway is blocked by a selective inhibitor, a SNAP-induced SVCT-2 increase is completely abolished; still, NO modulation of SVCT-2 expression is tightly associated with the NO-induced increase in SVCT-2 mRNA transcription because it is abolished by a transcriptional blocker and SNAP also increased the transcription of slc23a2 in cultured cells.
NO production and nNOS activity is tightly regulated by excitatory neurotransmission in cerebellar (38), retinal (27), and cortical neurons (39). Additionally, retinal excitatory neurotransmission has already been demonstrated to play a pivotal role in regulating ascorbate release (13). Therefore, the NO pathway is possibly recruited by diverse signaling systems to differentially regulate ascorbate availability at specific neuronal microenvironments such as the hippocampus, cerebellum, cortex, or retina. In chicken retinal mixed cultures, nNOS is exclusively expressed in neuronal cells (26). Interestingly, herein we demonstrated that iNOS expression in these cultures was completely absent in nonstimulated cells. On the other hand, eNOS is present in both neurons and glial cells in these cultures, but almost solely restricted to the nuclei of these cells. In line with this, it has been demonstrated that nuclear-localized NOS displays a very low NO generation capacity in COS-7 cells (40). For this, we advocate that the main source of NO in cultured chicken retinal cells is likely to be from nNOS-containing neurons.
Ascorbate is widely known by its intracellular antioxidant role (41). Conversely, extracellular ascorbate may induce oxidative stress, due to increased conversion to dehydroascorbate, and consequently promotes neuronal damage. In line with this,

SVCT-2 Regulation by Nitric Oxide
it has been shown that ascorbate induces apoptotic-like cell death in several cancerous lineages such as the human squamous carcinoma (SCaBER), the transitional cell papilloma (T24) (42), the T98G glioblastoma and TC-1 human renal carcinoma cell lines (43).
However, ascorbate within neuronal cells avoids ␣-tocopherol oxidation; increases the viability of SH-SY5Y cells when these cells are challenged with peroxide (44) or ␤-amyloid (45); inhibits MPP(ϩ)-induced cerebellar granule cells death (46); decreases ferricyanide-induced lipid peroxidation (47); and decreases H 2 O 2 -induced cell swelling in rat brain slices (48). Moreover, here we show a NO-induced regulation of ascorbate content to retinal cells and, specifically in the chicken retina, NO has been demonstrated to display a protective role against medium refeeding-induced neuronal cell death (21). Overall, the data presented herein raise the possibility that NO signals to increase intracellular ascorbate content in retinal neurons and this may protect retinal cells against oxidative stress-induced neuronal loss; furthermore, it also spans the possible intracellular mechanisms by which NO exerts its neuroprotective functions in the retina. Nevertheless, the linkage between the ascorbate transport system and NO signaling pathway in the intact retinal tissue may highlight the physiological importance of ascorbate as a signaling molecule in the nervous system and may integrate ascorbate to long-term responses in neuronal cells.
NO Signaling to Transcriptional Control-It has already been demonstrated that NO directly controls chromatin remodeling in cultured embryonic cortical neurons. This modulation is achieved by S-nitrosylation of histone deacetylase 2, which unbind the DNA and transcription may be turned on (49). Moreover, NO by means of S-nitrosylation regulates CREB/ DNA binding and consequently CREB-dependent transcription in cortical neurons (50). In addition, NO has also been reported to directly regulate CREB phosphorylation through the sGC/cGMP/PKG pathway in embryonic chick retinal neurons (27). Compelling evidence in the literature show that NO tightly regulates the NF-B signaling pathway in several experimental paradigms (51,52). For instance, NO S-nitrosylates the p50 subunit of the NF-B heterodimer decreasing its transcriptional activity, directly sensitizing prostate carcinoma cell lines to apoptosis (53). Moreover, NO S-nitrosylates IKK␤ at Cys-179 inhibiting it, which renders NF-B inactive in the cytoplasm (54). S-Nitrosylation also regulates p65/DNA binding activity and consequently NF-B-dependent gene regulation in inflammation (55).
Motionless, herein we presented strong evidence that SVCT-2 expression is under control of the NO/cGMP/PKG/ NF-B signaling pathway in cultured chicken retinal neurons. Moreover, NO induced both IB phosphorylation and NF-B translocation to the cell nucleus; suggesting that NO activated NF-B-dependent transcription to ultimately regulate SVCT-2 expression in cultured retinal cells. Therefore, we argue that NO modulates the activity of different sets of signaling molecules in our current model system to achieve altered signaling  FEBRUARY 3, 2012 • VOLUME 287 • NUMBER 6 outcomes, e.g. control of cell survival (21), cell proliferation (25), and availability of neuromodulators (data herein).

SVCT-2 Regulation by Nitric Oxide
NO and Control of SVCT-2 Synthesis: Role of NF-B Signaling-In the SVCT-2 gene there are consensus motifs for both NF-B and AP-1 transcription factors (7). Interestingly there are many reports in the literature showing that several signaling systems are related to NF-B activation; for instance, A2a adenosine receptors (56), TGF␤ receptors (57), NMDA receptors (58), and muscarinic receptors (59). Not surprisingly, all of these systems have already been correlated in some way with NO production (38, 60 -62). It has also been demonstrated that NO/cGMP/PKG is capable of modulating NF-B activation in cerebellar granule cells (52).
These findings are in high accordance with the data presented here showing that NO increases NF-B transcriptional activity through a cGMP-and PKG-dependent manner in cultured retinal cells. From our knowledge, it is the first time that a positive regulation is demonstrated by NO of NF-B activa-tion in retinal cells as well as a suggested role of PKG/NF-B in ascorbate transport. Moreover, this integrated signaling system may control Slc23a2 transcription and consequently SVCT-2 production. We suggest that the SVCT-2 protein is incorporated into the cellular membrane from its constitutive turnover, i.e. production (transcription and translation), transport (allocation and insertion), and operation in the plasmatic membrane (uptake); obviously with other levels of regulation in these processes. This suggestion is corroborated by our kinetics studies demonstrating that both SNAP and L-arginine increased the transport capacity for ascorbate (V max ) but not the transport affinity (K m ) of SVCT-2 for ascorbate. Furthermore, it is unlikely that NO promoted the insertion of preexisting transporters (allocated in cytoplasmic vesicles) into the plasmatic membrane, because SNAP-induced ascorbate uptake was completely abolished by inhibiting both SVCT-2 transcription with actinomycin D and translation with either anisomycin or CHX; in addition, qRT-PCR data strongly Next, samples were prepared for Western blotting. Results represent the mean Ϯ S.E.M. of independent experiments, all carried out in duplicates. In B: control (n ϭ 9), SNAP (n ϭ 9), SNAP ϩ Sulfa (n ϭ 3), Sulfa (n ϭ 3), SNAP ϩ PDTC (n ϭ 3), PDTC (n ϭ 3). In C, control (n ϭ 3), L-arginine (n ϭ 3), L-Arg ϩ Sulfa (n ϭ 3), Sulfa (n ϭ 3), L-Arg ϩ PDTC (n ϭ 3), PDTC (n ϭ 3). Cultures were also preincubated with PDTC (100 M) or sulfasalazine (100 M) for 10 min. Then, cells were incubated with SNAP (100 M) (D) or L-arginine (2 mM) (E) either in presence or absence of the mentioned compounds for an additional 10 min. Next, cells were pulsed with [ 14 C]ascorbate (0.1 Ci/ml) for 40 min, lysed with 5% TCA (w/v), and the radioactivity was measured. Results represent the mean Ϯ S.E.M. of different and independent experiments, all carried out in triplicates. In D, SNAP (n ϭ 17), SNAP ϩ PDTC (n ϭ 3), PDTC (n ϭ 3), SNAP ϩ Sulfa (n ϭ 3), Sulfa (n ϭ 3). In E, L-arginine (n ϭ 7), L-Arg ϩ PDTC (n ϭ 3), PDTC (n ϭ 6), L-Arg ϩ Sulfa (n ϭ 3), Sulfa (n ϭ 3). ***, p Ͻ 0.001 (relative to control); **, p Ͻ 0.01 (relative to control). CT, control.
suggest that SNAP increases mRNA content for SVCT-2 in our culture system. For these reasons, we suggest that NO promotes a de novo synthesis of SVCT-2 using PKG and NF-B as transducing systems to increase ascorbate uptake in cultured retinal cells. However, we cannot completely rule out the possibility that NO-activated PKG modulates either the traffic or the activity of SVCT-2 by phosphorylation. Consensus motifs for PKC phosphorylation in the SVCT-2 protein have been described (3, 4); so whether PKG or even NO itself (by means of S-nitrosylation) exert some sort of post-translational control in SVCT-2 activity remains to be established and requires further investigation.
Overall, we have demonstrated that the NO signaling pathway is directly related with SVCT-2 transporter expression and, consequently, with ascorbate uptake in retinal cells (Fig. 10). Given that SVCT-2 promoter activity is under the control of NF-B (7), in such a way, our data suggest that NO-regulated SVCT-2 expression and ascorbate uptake are achieved by a cGMP/PKG-dependent regulation of NF-B transcriptional function.