Interferon-inducible Transmembrane Protein 3 (IFITM3) Restricts Reovirus Cell Entry*

Background: The interferon-stimulated gene (ISG) IFITM3 restricts endosomal entry of enveloped viruses. Results: IFITM3 also restricts entry of reovirus, a nonenveloped virus. Conclusion: IFITM3 alters endosomal function, either by delaying acidification or modulating proteolytic activity. Significance: IFITM3 may restrict other clinically relevant nonenveloped viruses that require endosomes for entry. Reoviruses are double-stranded RNA viruses that infect the mammalian respiratory and gastrointestinal tract. Reovirus infection elicits production of type I interferons (IFNs), which trigger antiviral pathways through the induction of interferon-stimulated genes (ISGs). Although hundreds of ISGs have been identified, the functions of many of these genes are unknown. The interferon-inducible transmembrane (IFITM) proteins are one class of ISGs that restrict the cell entry of some enveloped viruses, including influenza A virus. One family member, IFITM3, localizes to late endosomes, where reoviruses undergo proteolytic disassembly; therefore, we sought to determine whether IFITM3 also restricts reovirus entry. IFITM3-expressing cell lines were less susceptible to infection by reovirus, as they exhibited significantly lower percentages of infected cells in comparison to control cells. Reovirus replication was also significantly reduced in IFITM3-expressing cells. Additionally, cells expressing an shRNA targeting IFITM3 exhibited a smaller decrease in infection after IFN treatment than the control cells, indicating that endogenous IFITM3 restricts reovirus infection. However, IFITM3 did not restrict entry of reovirus infectious subvirion particles (ISVPs), which do not require endosomal proteolysis, indicating that restriction occurs in the endocytic pathway. Proteolysis of outer capsid protein μ1 was delayed in IFITM3-expressing cells in comparison to control cells, suggesting that IFITM3 modulates the function of late endosomal compartments either by reducing the activity of endosomal proteases or delaying the proteolytic processing of virions. These data provide the first evidence that IFITM3 restricts infection by a nonenveloped virus and suggest that IFITM3 targets an increasing number of viruses through a shared requirement for endosomes during cell entry.

ratory syndrome coronavirus, and filoviruses (11)(12)(13). These viruses are enveloped and enter cells via membrane fusion in endosomal compartments; indeed, the IFITM proteins restrict viruses independent of receptor usage but are dependent upon processes that occur in late endosomes (12,14). IFITM3 is the most potent IFITM family member in restricting influenza A replication in cell culture (11), and recently it has been shown to reduce the pathogenesis of influenza in mice (15,16) and in infected humans (16). Our recent data suggest a model where IFITM3 prevents influenza A virus fusion, thereby trapping virions within the endocytic pathway ultimately leading to their destruction in lysosomes and autolysosomes (14). Whether IFITM3 restricts non-enveloped viruses that also require endosomal access for cell entry is not known.
Mammalian orthoreoviruses (reoviruses) are non-enveloped viruses containing a segmented genome composed of dsRNA (17). Reoviruses utilize a multistep entry process, first binding to cell surface carbohydrates before engagement with the proteinaceous receptor junction adhesion molecule-A (JAM-A) (18). Subsequent interactions with ␤1-integrins target virions to late endosomal compartments (19), where the actions of acid-dependent cathepsin proteases are required for uncoating (20). These proteases cleave outer capsid protein 3, revealing the membrane penetration protein 1 (21)(22)(23)(24)(25). Cleavages of 1 into the particle-associated fragments ␦ and and the dissociated fragment 1N allow for conformational changes and subsequent membrane association of the viral capsid that mediates penetration of the viral core into the cytoplasm (23,26). Although reovirus particles traverse a number of endocytic compartments, correct targeting to late endosomes containing Rab7 and Rab9 is crucial for establishing a productive infection (27). Because IFITM3 also localizes to late endosomes, we sought to test whether IFITM3 restricts reovirus infection.
In this study we found that IFITM3 expression significantly restricts infection and replication by reovirus. IFITM3-mediated restriction occurs at the level of endosomal penetration, as neither receptor binding nor RNA synthesis was affected. Targeting of reovirus particles to Rab7-containing late endosomes occurred with similar kinetics in the presence or absence of IFITM3. However, the kinetics of acidification and subsequent proteolysis of the reovirus capsid were delayed in IFITM3-expressing cells. Thus, IFITM3 likely alters the dynamics of endosomal uncoating, either leading to inefficient membrane penetration or lysosomal degradation of viral particles. This represents the first evidence that IFITM3 can restrict the infection of non-enveloped viruses that utilize endosome-dependent cell entry mechanisms and provides further mechanistic evidence for how IFITM3 restricts an increasing number of viruses by targeting a shared requirement for endosomes for cell entry.
Reovirus strains type 1 Lang (T1L) and type 3 Dearing (T3D) are laboratory stock. Reovirus strain rsT3D-1T249I was recovered by plasmid rescue (28). rsT3D-1T249I is isogenic to T3D with the exception of a single amino acid substitution in the 1 protein rendering it insensitive to protease cleavage and, therefore, capable of generating infectious subvirion particles (ISVPs) with infectivity equivalent to that of T3D virions (29).
Purified reovirus virions were generated using second or third passage L-cell lysates of twice-plaque-purified reovirus as described (30). Viral particles were Freon-extracted from infected cell lysates, layered onto 1.2-1.4-g/cm 3 CsCl gradients, and centrifuged at 62,000 ϫ g for 18 h. Bands corresponding to virions (1.36 g/cm 3 ) were collected and dialyzed in virion storage buffer (150 mM NaCl, 15 mM MgCl 2 , 10 mM Tris-HCl, pH 7.4). Concentrations of reovirus virions in purified preparations were determined from an equivalence of 1 absorbance unit at 260 nm equals 2.1 ϫ 10 12 virions (31). Viral titer was determined by a plaque assay using murine L929 cells, and all indications of m.o.i. are based on L929 cell titer (32). ISVPs were generated as described (33) and confirmed by SDS-PAGE and Coomassie Brilliant Blue staining. Guinea pig anti-reovirus NS and rabbit anti-T3D antisera were generously provided by Dr. Terence S. Dermody (Vanderbilt University Medical Center). Plasmids encoding Rab7-EGFP and Rab-interacting lysosomal protein (RILP)-EGFP (27) and guinea pig anti-reovirus NS antiserum were obtained from Dr. Terence S. Dermody (Vanderbilt University Medical Center).
Fluorescent Focus Assay-Cells (4 ϫ 10 4 ) were grown in 24-well tissue culture plates and adsorbed with reovirus strains at various m.o.i. for 1 h at 4°C. After adsorption, 0.5 ml of fresh medium was added, and the cells were incubated at 37°C for 18 h. Cells were fixed with 2% paraformaldehyde for 30 min, washed twice with PBS, and permeabilized and blocked in PBS ϩ 2% bovine serum albumin ϩ 0.1% Triton-X100 (PBS-T) for at least 1 h at 4°C. Cells were then incubated with guinea pig anti-reovirus NS antiserum (1:1000) in PBS-T for at least 1 h at 4°C, washed 3ϫ with PBS-T, and incubated with an antiguinea pig Alexa 568-conjugated antibody (1:2000) in PBS-T for at least 1 h at 4°C. Cells were washed 3ϫ with PBS and visualized using fluorescence microscopy. Reovirus antigenpositive cells were quantified by counting fluorescent cells in at least two random fields of view in triplicate wells at a magnification of 113ϫ. Total cell number was quantified using background fluorescence.
Assessment of Virus Replication by Plaque Assay-Cells (4 ϫ 10 4 ) grown in 24-well tissue culture plates were adsorbed with reovirus strain T3D at an m.o.i. of 1 or 100 pfu/cell for 1 h at 4°C. After adsorption, cells were washed 2ϫ with PBS and incubated in 0.5 ml of fresh medium at 37°C. After different time intervals, cells were freeze-thawed twice, and viral titer was determined by plaque assay. The viral yields were calculated by dividing viral titers at the indicated times by the viral titer at 0 h.
Analysis of Interferon Sensitivity-Cells (4 ϫ 10 4 ) grown in 24-well tissue culture plates were either mock-treated or treated with IFN-␣ (100 IU/ml) for 6 h before inoculation with either T1L or T3D at an m.o.i. of 10 pfu/cell for 1 h at 4°C. After inoculation, 0.5 ml of fresh medium was added, and the cells were incubated at 37°C for 18 h before performing a fluorescent focus assay. Alternatively, viral titers at various times were determined by plaque assay.
Endosomal Acidification Inhibition-Cells (4 ϫ 10 4 ) grown in 24-well tissue culture plates were adsorbed with either rsT3D-1T249I virions or ISVPs at the indicated m.o.i. for 45 min at 4°C. After adsorption, cells were either mock-treated or treated with 10 mM ammonium chloride and incubated at 37°C for 18 h and analyzed by fluorescent focus assay.
Kinetic Ammonium Chloride Protection Assay-Cells (4 ϫ 10 4 ) grown in 24-well tissue culture plates were inoculated with rsT3D at an m.o.i. of 25 pfu/cell for 1 h at 4°C. The inoculum was removed, and the cells were washed 2ϫ with PBS and incubated in fresh prewarmed media at 37°C. At various times after adsorption, ammonium chloride was added to the medium for a final concentration of 25 mM. Cells were then incubated for 18 h post-adsorption, fixed with 2% paraformaldehyde, and stained for a fluorescent focus assay.
Analysis of Viral Disassembly-Cells (5 ϫ 10 5 ) grown in 60-mm dishes were inoculated with T3D at an m.o.i. of 100 pfu/cell for 1 h at 4°C. The inoculum was removed, and the cells were washed 2ϫ with PBS and incubated in fresh prewarmed media at 37°C. At various times after adsorption, cells were scraped into 1 ml of ice-cold PBS and pelleted at 3000 ϫ g for 5 min at 4°C. The supernatant was aspirated, and the pellet was resuspended in 100 l of ice-cold modified radioimmunoprecipitation assay buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% sodium deoxycholate, 1% IGEPAL CA-630, 1 mM PMSF) supplemented with protease inhibitor mixture (Roche Applied Science). The lysate was clarified by centrifugation at 13,000 ϫ g for 10 min at 4°C, and the supernatant was removed to a fresh tube and frozen at Ϫ20°C. Extracts (10 g of total protein) were resolved by electrophoresis in 4 -12% Bis-Tris gels and transferred to PVDF membranes. The membranes were blocked overnight at room temperature in PBS ϩ 1% Tween 20 (PBS-T) containing 5% milk and incubated with a rabbit anti-T3D antiserum (1:500) in PBS-T plus milk at room temperature for 3 h. The membranes were washed 3ϫ for 10 min with PBS-T and incubated with an alkaline phosphataseconjugated goat anti-rabbit antibody (Bio-Rad) diluted 1:2000 for 3 h. After 3 washes with PBS-T, the membranes were incubated for 5 min with chemiluminescent alkaline phosphate substrate (Bio-Rad) and visualized using a ChemiDock XRSϩ molecular imager (Bio-Rad). Band densities were analyzed using Image J software.
Quantitative Reverse Transcriptase Polymerase Chain Reaction (qPCR)-Cells (5 ϫ 10 5 ) grown in 60-mm dishes were adsorbed with T3D in PBS at an m.o.i. of 100 pfu/cell at 4°C for 1 h. Cells were incubated in medium at 37°C for various intervals, removed from plates with a scraper, washed once with PBS, and centrifuged at 500 ϫ g for 5 min. The supernatant was removed, and the cell pellet was frozen at Ϫ20°C. RNA was extracted by using an RNeasy Plus RNA extraction minikit (Qiagen) according to the manufacturer's instructions. RNA was converted to cDNA by using an Omniscript RT cDNA synthesis kit (Qiagen) with an oligo(dT) primer according to the manufacturer's instructions. qPCR was performed using the Express SYBR Green ER system (Invitrogen). Primers specific for human GAPDH (forward primer 5Ј-GATCATCAGCAAT-GCCTCCT-3Ј and reverse primer 5Ј-TGTGGTCATGAGTCC-TTCCA-3Ј) or human IFITM3 (forward primer 5Ј-ATGTCGTC-TGGTCCCTGTTC-3Ј and reverse primer 5Ј-GTCATGAGGA-TGCCCAGAAT-3Ј) were used at a final concentration of 0.2 M. Quantification and melt curve analyses were performed according to the manufacturer's protocol. For each sample, the CT (threshold cycle) for the RNA of interest was normalized to that for GAPDH. -Fold induction was calculated by comparing normalized CT values (⌬⌬CT) of duplicate cDNA synthesis reactions to those of samples taken at the time of infection (T ϭ 0) for two independent experiments.
Confocal Microscopy of Reovirus Internalization-Cells were plated on glass coverslips (#1.5; Thermo Scientific) in 24-well plates at 37°C overnight. Cells were transfected with plasmids using Lipofectamine 2000 (Life Sciences) according to the manufacturer's instructions. After incubation at 37°C for 18 h, the cells were chilled at 4°C for 1 h. Cells were then adsorbed with 10,000 Alexa546-labeled reovirus particles/cell at 4°C for 1 h. The inoculum was removed, and the cells were washed 3 times with PBS and either fixed with 2% paraformaldehyde or supplemented with complete medium and incubated at 37°C for various intervals. Cells were washed once with PBS and fixed for 20 min with 2% paraformaldehyde, quenched with 0.1 M glycine, and washed 3 times with PBS. Coverslips were removed from wells and placed on slides using ProLong Gold mounting medium (Life Sciences). Images were captured using a Zeiss LSM710 laser-scanning confocal microscope using a 63ϫ Plan-Apochromat objective lens. Images were thresholded for pixel intensity, and the pinhole size used was identical for all fluorophores. All images represent single sections and were adjusted for brightness and contrast to the same extent. Colocalization analysis was performed using the colocalization function of Zeiss LSM image software (Zeiss) taking into consideration endosomal vesicle size to try to isolate individual endosomes. Virions within the boundary of single cells were quantified.
Assessment of pHrodo Fluorescence Intensity by Flow Cytometry-The kinetics of reovirus access to acidic compartments was performed as described previously (27). HeLa cells were chilled at 4°C for 1 h and adsorbed with pHrodo-labeled reovirus (1 ϫ 10 4 particles/cell) at 4°C for 1 h. The inoculum was removed, and the cells were washed 3 times with cold PBS to remove unbound virus and supplemented with warm Opti-MEM-I (Invitrogen) for various intervals. The cells were washed once with PBS, detached with Cellstripper (Corning CellGro), washed once with PBS, and analyzed with a BD Accuri C6 flow cytometer. Cell staining was quantified using BD Accuri C6 software.

RESULTS
IFITM3 restricts entry of enveloped viruses that require acid pH or endosomal components for membrane fusion (12,14). As the mechanism for this restriction is not defined, we tested whether IFITM3 also restricts entry of a non-enveloped virus with similar membrane penetration requirements, mammalian orthoreovirus. We utilized previously developed HeLa and U2OS cell lines that were engineered to stably express IFITM3 (11). Empty vector-transduced cell lines served as controls. Expression levels of IFITM3 in mock-and IFN-␣-treated cells were determined by qPCR and by immunoblotting ( Fig. 1 and data not shown). As previously observed, control HeLa cells express basal levels of IFITM3 in the absence of IFN treatment FIGURE 1. IFITM3 expression in HeLa cell lines. Vector control, IFITM-3-expressing, and shIFITM3-expressing HeLa cell lines were mock-treated or treated with 100 IU/ml IFN-␣ for 6 h. Cultures were scraped into PBS and pelleted. RNA was extracted from cultures, converted into cDNA, and used for qPCR analysis using primers specific for IFITM3 (top panel). The level of IFITM3 RNA was normalized to that of GAPDH in each culture. Alternatively, cultures were lysed with radioimmunoprecipitation buffer and subjected to SDS-PAGE electrophoresis followed by immunoblotting using antisera against IFITM3 (bottom panel).   (11,16). IFITM3-expressing cells produced ϳ4-fold more IFITM3 than mock-treated cells and ϳ2-fold more IFITM3 than IFN-␣-treated cells. IFITM3-expressing and control cell lines were adsorbed with reovirus strains T1L and T3D at various m.o.i. (as determined using L929 cells), and the percentage of infected cells was determined at 18 h post-infection by immunostaining using an anti-reovirus NS antiserum. Reovirus infection was significantly reduced in cells expressing IFITM3 (Fig. 2). The reduction in infection was greater at lower m.o.i., suggesting that the restriction mediated by IFITM3 could be saturated at high m.o.i. Similar results were obtained using an A549 cell line engineered to express IFITM3 (data not shown).
We next tested whether the decrease in the percentage of infected cells mediated by IFITM3 would result in decreased viral replication. IFITM3-expressing and control cell lines were infected with T3D at an m.o.i. of 1 pfu/cell, which allows for the initially infected cells to produce type I IFNs and prime uninfected cells for increased IFITM3 expression. Viral yield was then determined at various times post-infection. IFITM3 expression in HeLa and U2OS cell lines resulted in a 5-to-10fold decrease (p Ͻ 0.05) in viral yield in comparison to vector control cells at all times examined (Fig. 3, A and C). Decreased yields were also observed in IFITM3-expressing HeLa cells infected at an m.o.i. of 100 pfu/cell, which would not allow for IFN-mediated priming, indicating that basal levels of IFITM3 can still restrict infection at high infectious doses of reovirus (Fig. 3B). These data suggest that IFITM3 overexpression restricts, but does completely inhibit, reovirus infection.
To determine whether IFITM3 is induced by reovirus infection, we first measured IFITM3 RNA levels in HeLa cells after infection. Cells were mock-infected or infected with T3D at an m.o.i. of 100 pfu/cell, and RNA was extracted from cells at various times post-infection. Levels of IFITM3 mRNA were quantified by using qPCR. We observed a continuous, time-dependent increase in IFITM3 expression after reovirus infection, with a maximum of a ϳ5-fold increase over base-line expression after 24 h (Fig. 4A). Increased IFITM3 expression was first observed at 12 h post-infection, a result that is consistent with its known dependence on IFN for expression. Similar results were observed at the level of protein expression, as determined by immunoblot (Fig. 4B). We then utilized HeLa cells constitutively expressing a short hairpin RNA targeting IFITM3 (shIFITM3) to determine if IFITM3 expression could restrict reovirus infection in IFN-treated cells. HeLa cells expressing shIFITM3 exhibited reduced IFITM3 expression by ϳ4 -5-fold at both the RNA and protein level (Fig. 1). Control and shIFITM3-expressing HeLa cells were treated with 100 IU of IFN-␣ for 6 h before infection with T1L or T3D. This level of interferon is similar to that produced by reovirus infection of cultured fibroblasts (35,36). The percentage of infected cells was determined by immunostaining at 18 h post-infection. Cells expressing shIFITM3 were significantly less sensitive to the antiviral effects of IFN in comparison to control cells (Fig. 3,  C and D), as IFN treatment reduced the percent of infected cells by 60 -80% in control cells and by 10 -20% in shIFITM3-expressing cells (p Ͻ 0.05). In productively infected cells, however, no differences were observed in the number or size of NS-containing inclusions (data not shown). No significant difference was observed in the percentage of infected controland shIFITM3-expressing cells in the absence of IFN (p ϭ 0.12). We also examined reovirus replication in mock-and IFN-␣treated control cells and shIFITM3-expressing cells. IFN-␣ treatment significantly decreased reovirus replication in control cells (Fig. 4E, p Ͻ 0.05) but had no effect on replication in shIFITM3-expressing cells, suggesting that IFITM3 expression can restrict reovirus replication in cells responding to type I IFN.
We next sought to determine at which stage of the reovirus replication cycle IFITM3 mediates its effects. Because IFITM3 localizes to endosomal compartments, we hypothesized that it would act at the virion disassembly stage in late endosomes and not at the level of receptor engagement and target cell binding. To test this, we utilized ISVPs, which bypass the requirement for endosomal proteases for cell entry (21). Therefore, ISVPs, unlike virions, are not sensitive to the weak base ammonium chloride, which prevents endosomal acidification. Control-or IFITM3-expressing cells were adsorbed with rsT3D-1T249I virions or ISVPs and then incubated in the presence or absence of ammonium chloride for 18 h, at which time the percentage of infected cells was determined by immunostaining. Ammonium chloride treatment reduced the infectivity of virions by ϳ80%, with IFITM3-expressing cells exhibiting lower levels of infection than control cells (Fig. 5). In contrast, ammonium chloride had no effect on the entry of ISVPs, and no differences were observed in the percentage of infected cells in the presence or absence of IFITM3. Similar results were observed in controland IFITM3-expressing U2OS cell lines (data not shown). Additionally, no differences were observed in the initial binding of virions to controlversus IFITM3-expressing cells as determined by flow cytometry using fluorescently tagged virions (not shown). Together, these data indicate that IFITM3 acts at a stage subsequent to initial receptor binding on target cells, likely during endosomal trafficking.
IFITM3 may function in endosomes to restrict reovirus entry via several possible mechanisms. One possibility may be that IFITM3 directs internalized virions to cellular compartments that are non-productive for the uncoating process, as incorrectly targeted virions are incapable of mediating infection (37). Productive reovirus entry occurs in late endosomes that colocalize with specific Rab GTPases such as Rab7 and RILP (27). We examined whether IFITM3 altered reovirus targeting to late endosomes by utilizing GFP-tagged endosomal markers and fluorescently tagged virions. Control-or IFITM3-expressing HeLa cells were transfected with Rab7-EGFP or RILP-EGFP, which localize to late endosomes (38,39). At 18 h posttransfection, cells were chilled at 4°C for 1 h, adsorbed with A546-labeled reovirus particles at 4°C for 1 h, and incubated at 37°C for 0, 60, or 120 min. At these times, cells were fixed with paraformaldehyde and visualized by confocal microscopy (Fig.  6A). Quantification of colocalization between reovirus particles and either Rab7-EGFP or RILP-EGFP showed no significant differences between control-and IFITM3-expressing cells (Fig.  6, B and C). These data suggest that IFITM3 does not alter virion targeting to late endosomal compartments.
Because endosomal targeting is not affected, we hypothesized that IFITM3 alters the efficiency and/or timing of virion disassembly. To better gauge the efficiency of entry events, we took several approaches. First, we determined whether IFITM3 alters the kinetics with which reovirus particles escape host cell endosomes using an ammonium chloride bypass assay. This assay is predicated upon the observation that reovirus disassembly is prevented by inhibiting the pH decrease required for efficient cathepsin cleavage, whereas intact particles are still resident in endosomes (21,40). We adsorbed monolayers of control-and IFITM3-expressing HeLa cells with rsT3D at 4°C for 1 h, washed the cells with PBS, and added prewarmed DMEM. Cells were incubated at 37°C and ammonium chloride was added at various intervals to prevent endosome acidification. Cells were incubated overnight and scored for the percentage of infected cells by indirect immunofluorescence (Fig.  7). rsT3D escaped ammonium chloride blockade around 30 min earlier (p Ͻ 0.05) in control cells than in IFITM3-expressing cells, which indicates that IFITM3 delays the kinetics of endosomal escape.
Next, we determined the kinetics of reovirus particles entering into acidic endosomal compartments. Virus particles were labeled with pHrodo, a fluorophore that fluoresces at a pH less than 5. Control-or IFITM3-expressing HeLa cells were chilled for 1 h at 4°C, adsorbed with pHrodo-labeled particles for 1 h at 4°C, incubated in prewarmed media at 37°C for various times, and then assayed for fluorescence intensity by flow cytometry. Fluorescence intensity increased in both control-and IFITM3expressing cells over time; however, the kinetics of increase in fluorescence intensity was slower in the presence of IFITM3 (p Ͻ 0.05; Fig. 8). Because colocalization between virus particles and late endosomes occurred with similar kinetics in controland IFITM3-expressing cells, these data suggest that IFITM3 may function to alter the rate of acidification of late endosomal compartments.
Finally, we examined the kinetics of outer capsid proteolysis in control-and IFITM3-expressing HeLa cells. Cells were adsorbed with virions for 1 h at 4°C and then incubated at 37°C for 0, 60, 90, or 120 min. At these time points, cells were lysed in radioimmunoprecipitation buffer, and the lysates were analyzed by SDS-PAGE electrophoresis followed by immunoblotting using a T3D-specific antiserum. Cells expressing IFITM3 exhibited delayed cleavage of 1 into 1C and ␦ in comparison to control cells (Fig. 9), with initial cleavage products detected in control cells by 60 min post-infection and in IFITM3-expressing cells by 90 min post-infection. Together with data regarding endosome acidification and the kinetics of ammonium chloride bypass, these data indicate that IFITM3 restricts reovirus entry by altering the efficiency of endosomal acidification and subsequent protease activity, which serves to alter the kinetics of outer capsid proteolysis and membrane penetration.

DISCUSSION
The type I IFN system works to restrict viral infection through many mechanisms. Requirements shared by many viruses in their replication cycle provide advantageous targets for cellular antiviral mechanisms, as a single protein or family of proteins may prove efficacious against a large number of potential pathogens. Cell entry represents a very attractive target, as all viruses require some mechanism to penetrate into a host cell either at the cell surface, via endosomes, or via other cell fusion or cell transport events. However, because viruses use a wide variety of different receptors, targeting receptor binding or adhesion may not be effective against a broad range of pathogens. In contrast, many viruses, both enveloped and non-enveloped, require the use of endosomal compartments either for proteolytic cleavage or pH-dependent conformational changes necessary for membrane fusion or penetration (41,42). Hence, evolving the capacity to prevent viruses from penetrating into the cytoplasm from endosomes represents a key antiviral response. The IFITM proteins, and in particular IFITM3, fulfill this function by restricting endosomal viral entry mechanisms.
IFITM3 is a 133-amino acid membrane-associated protein, and its expression is increased 4 -40-fold by type I IFN (Refs. 11, 43, and 44; Fig. 1). The topology of IFITM3 in membranes is not fully understood. It was originally characterized as having two transmembrane domains, with the N and C termini facing the lumen of the ER and endosomes (10,11,46). However, recent evidence suggests that it may be an intramembrane protein, with the protein termini oriented toward the cytoplasm (47). IFITM3 is S-palmitoylated (47,48), and palmitoylation enhances the antiviral activity of the protein. Recent mechanistic data indicate that IFITM3 may localize to endosomes con-   A, HeLa vector control-or IFITM3-expressing cells were adsorbed with T3D at an m.o.i. of 100 pfu/cell for 1 h at 4°C. Cells were washed 2 times with PBS and incubated with prewarmed media at 37°C. At the times shown after adsorption, cells were scraped into PBS, pelleted at 3000 ϫ g, and resuspended in radioimmunoprecipitation buffer. Lysates were clarified by centrifugation at 13,000 ϫ g, resolved in 4 -12% polyacrylamide gels, and transferred to PVDF membranes. The membranes were probed with a rabbit polyclonal anti-T3D antiserum and an anti-rabbit AP-conjugated secondary antibody and visualized using chemiluminescence. B, bands corresponding to 1C and ␦ from three individual experiments were quantified using ImageJ software and normalized using nonspecific background bands. *, p Ͻ 0.05 by Student's t test in comparison with vector control cells.

IFITM3 Restricts Reovirus Entry
taining the vacuolar ATPase proton pump ATP6v0b, leading to alterations in the endosomal compartments including increased association with clathrin (49).
The precise mechanism of action for IFITM3 is not fully understood. Several possible mechanisms have been proposed previously. IFN treatment or IFITM3 expression expands late endosomal and autolysosomal compartments (14,49), indicative of changes in the activity of these structures. These changes could alter the rate of acidification of endosomes before delivery of the components to lysosomes, influence cathepsin activity (either due to changes in the pH or by directly affecting catalytic activity), or modify endosomal membrane characteristics, such as elasticity or curvature, to prevent membrane fusion. The latter hypothesis was strengthened by recent evidence that IFITM3 alters membrane fluidity to prevent hemifusion mediated by viral fusion proteins (50).
Our data indicate that IFITM3 acts in late endosomes to restrict reovirus cell entry. Although differences in m.o.i. and detection mechanisms make direct comparisons difficult, the level of restriction mediated by IFITM3 against reovirus is similar to that observed for enveloped viruses, including influenza (11), HIV-1 (51), and West Nile Virus (13). Reovirus particles colocalize with late endosomal markers with the same kinetics in the presence or absence of IFITM3, indicating that IFITM3 does not alter entry into endosomal compartments. Rather, particles access an acidic environment later in IFITM3-expressing cells, suggesting that IFITM3 alters the kinetics of endosomal acidification. This is consistent with the observation that ISVPs are not sensitive to IFITM3; ISVPs require endosomes for entry but do not require endosomal acidification (21,52). Thus, uncoating is delayed, and virions may be delivered to lysosomes or other degradative compartments before they can complete membrane penetration. Accordingly, a previous study indicated that particles that do not efficiently penetrate endosomes localize to lysosomes (23). We cannot exclude the possibility that IFITM3 also alters the properties of endosomal membranes to prevent membrane penetration. Although reovirus does not possess a fusion protein like enveloped viruses, it does form pores in membranes to facilitate entry, and alterations to membrane curvature, elasticity, or bending modulus may alter the dynamics of pore formation and prevent entry.
We observed that IFITM3 expression accounts for between 60 and 80% of the decrease in the percentage of reovirus-infected cells mediated by IFN-␣ treatment. The fluorescent focus assay used for this experiment primarily assesses how many cells become productively infected, and not other aspects of the viral replication cycle, such as levels of RNA or protein synthesis. Therefore, these data suggest that other ISGs, including possibly other members of the IFITM protein family, may restrict reovirus entry. Other ISGs, including MxA (53) and PKR (protein kinase R) (54,55) can restrict reovirus infection but primarily act on post-entry events such as protein synthesis. The relative efficacy of each individual ISG in restricting reovirus replication remains to be determined.
Inhibiting expression of IFITM3 after IFN treatment had a relatively greater effect in allowing T3D infection in comparison to T1L. Therefore, IFITM3 could at least partially account for the known differences in IFN sensitivity between T3D and T1L (56,57). T3D ISVPs are less stable than T1L ISVPs (58) and, therefore, may be more sensitive to changes in the endosomal compartment during uncoating. This phenotype is linked to the M2 gene, particularly a Ala/Val change at position 305 in the T3D 1 protein (59,60). This residue is located in the ␦ region of 1, which forms the central region of the protein and undergoes conformational changes during uncoating (23). Whether alterations in this region of the protein also affect sensitivity to IFITM3 is unknown.
Perhaps the most intriguing implication of our investigation is that restriction by IFITM3 is not limited to enveloped viruses that penetrate via endosomes but may include nonenveloped viruses with similar requirements for entry as well. Many nonenveloped viruses require access to endosomal compartments for membrane penetration, including parvoviruses (61,62), adenoviruses (63,64), and some rotaviruses (65). However, the precise endosomal dynamics required for these viruses to penetrate membranes differ. Rhesus rotavirus only requires early endosomes for cell entry (66), whereas adenovirus and canine parvovirus require acid-dependent activities in late endosomes (45,62,67,68). Thus, determining the full range of viruses that are sensitive to IFITM3 expression may provide further insight into its precise mechanism and location of action and provide useful information in developing broad-spectrum antiviral therapies.
The IFITM protein family, and IFITM3 in particular, represent an important interferon-inducible antiviral response. Recent reports demonstrating that IFITM3 limits the pathogenesis of influenza infection in mice (15,16) and in humans (16) highlight the relevance of this pathway for innate immunity. Our data suggest that IFITM3 may play a significant role in modulating the pathogenesis of nonenveloped viruses as well, which could inform novel therapies for an expanded range of clinically relevant pathogens.