ubiI, a New Gene in Escherichia coli Coenzyme Q Biosynthesis, Is Involved in Aerobic C5-hydroxylation*

Background: The C5-hydroxylation reaction of coenzyme Q biosynthesis in Escherichia coli is catalyzed by an unknown enzyme. Results: The UbiI protein is responsible for the C5-hydroxylation reaction. Conclusion: The three monooxygenases involved in aerobic Q biosynthesis are now identified. Significance: We report the characterization of a gene of unknown function and the first crystal structure of a monooxygenase involved in Q biosynthesis. Coenzyme Q (ubiquinone or Q) is a redox-active lipid found in organisms ranging from bacteria to mammals in which it plays a crucial role in energy-generating processes. Q biosynthesis is a complex pathway that involves multiple proteins. In this work, we show that the uncharacterized conserved visC gene is involved in Q biosynthesis in Escherichia coli, and we have renamed it ubiI. Based on genetic and biochemical experiments, we establish that the UbiI protein functions in the C5-hydroxylation reaction. A strain deficient in ubiI has a low level of Q and accumulates a compound derived from the Q biosynthetic pathway, which we purified and characterized. We also demonstrate that UbiI is only implicated in aerobic Q biosynthesis and that an alternative enzyme catalyzes the C5-hydroxylation reaction in the absence of oxygen. We have solved the crystal structure of a truncated form of UbiI. This structure shares many features with the canonical FAD-dependent para-hydroxybenzoate hydroxylase and represents the first structural characterization of a monooxygenase involved in Q biosynthesis. Site-directed mutagenesis confirms that residues of the flavin binding pocket of UbiI are important for activity. With our identification of UbiI, the three monooxygenases necessary for aerobic Q biosynthesis in E. coli are known.


Coenzyme Q (ubiquinone or Q) is a redox-active lipid found in organisms ranging from bacteria to mammals in which it plays a crucial role in energy-generating processes. Q biosynthesis is a complex pathway that involves multiple proteins.
In this work, we show that the uncharacterized conserved visC gene is involved in Q biosynthesis in Escherichia coli, and we have renamed it ubiI. Based on genetic and biochemical experiments, we establish that the UbiI protein functions in the C5-hydroxylation reaction. A strain deficient in ubiI has a low level of Q and accumulates a compound derived from the Q biosynthetic pathway, which we purified and characterized. We also demonstrate that UbiI is only implicated in aerobic Q biosynthesis and that an alternative enzyme catalyzes the C5-hydroxylation reaction in the absence of oxygen. We have solved the crystal structure of a truncated form of UbiI. This structure shares many features with the canonical FAD-dependent para-hydroxybenzoate hydroxylase and represents the first structural characterization of a monooxygenase involved in Q biosynthesis. Site-directed mutagenesis confirms that residues of the flavin binding pocket of UbiI are important for activity. With our identification of UbiI, the three monooxygenases necessary for aerobic Q biosynthesis in E. coli are known.
Coenzyme Q (ubiquinone or Q) 3 is a widespread redox-active lipid essential for electron and proton transport in the respiratory chains from bacteria to mammals (1,2). Q is composed of a fully substituted benzoquinone ring to which is attached a polyisoprenyl tail with a number of isoprenyl units varying from one species to another (6 in Saccharomyces cerevisiae, 10 in humans, and 8 in Escherichia coli). Biosynthesis of Q is a highly conserved pathway that involves a large number of genes that have been identified mostly from seminal genetic studies using S. cerevisiae and E. coli (3,4). However, our genetic understanding of Q biosynthesis is still incomplete, and very few Q biosynthetic proteins have been isolated and characterized biochemically.
Biosynthesis of Q 8 in E. coli requires 9 Ubi proteins (UbiA-H and UbiX); most of them are involved in reactions that modify the aromatic ring of the 4-hydroxybenzoic acid (4-HB) universal precursor (Fig. 1). After prenylation of 4-HB catalyzed by UbiA, UbiX and UbiD are proposed to be involved in the decarboxylation (5). Three methylation (reactions d, f, and h, Fig. 1) and three hydroxylation reactions (on C5, C1, and C6, reactions c, e, and g, respectively) follow to yield Q 8 . UbiG catalyzes both O5-and O6-methylation (6) whereas UbiE is the C2-methyltransferase (7). The genes encoding the enzymes responsible for the aerobic C1-and C6-hydroxylation reactions have been identified as ubiH and ubiF, respectively (8,9) (Fig. 1 UbiH share 32% of sequence identity and are defined as members of class A flavoprotein monooxygenases from sequence analysis (10), even though they have not been isolated in pure form and characterized in vitro. In contrast, the gene responsible for the C5-hydroxylation reaction in E. coli (reaction c on Fig. 1) remains unknown. UbiB has been proposed to be involved in this reaction based on the accumulation of OPP (Fig. 1) in a ubiB Ϫ strain (11,12). However, accumulation of OPP does not necessarily reflect a defect in C5-hydroxylation because a strain deficient in the methyltransferase UbiG also accumulates OPP (13). Moreover, the primary sequence of UbiB does not contain any motif characteristic for hydroxylases but instead displays conserved motifs found in a superfamily of ancestral kinases (14). Searching the gene responsible for the C5-hydroxylation, we reasoned that visC, a gene of unknown function, could be a good candidate to investigate because it locates immediately downstream of ubiH within the same operon (9). Furthermore, visC is predicted to encode a protein with the typical domains shared by flavoprotein monooxygenases and with 30 and 39% sequence identity to UbiH and UbiF, respectively (supplemental Fig. S1) (10). In the present study, we report genetic and biochemical experiments that unambiguously establish that VisC participates in coenzyme Q 8 biosynthesis by catalyzing the aerobic C5-hydroxylation reaction. We therefore propose to change the name of VisC into UbiI. We succeeded in purifying the protein and crystallizing a truncated form of UbiI. The crystal structure shows a high degree of similarity with that of para-hydroxybenzoate hydroxylase (PHBH) a well characterized FAD-dependent monooxygenase (15). Albeit incomplete, our structure of UbiI is the first crystal structure of a monooxygenase associated with ubiquinone biosynthesis.

EXPERIMENTAL PROCEDURES
Strains and Growth Conditions-E. coli strains were grown in Luria-Bertani (LB)-rich medium at 37°C. Anerobic cultures were performed in sealed 15-ml Falcon tubes filled to the top with culture medium. Strains used in this study are listed in supplemental Table S1. Ampicillin (50 g/ml) and kanamycin (25 g/ml) were added when necessary. All of the strains are E. coli K-12 derivatives. Strains JW2874, JW2875, JW0659, and JW5581 from the Keio collection (16) were kindly provided by P. Moreau (LCB, Marseille) and were the donor of the ⌬ubiI::kan, ⌬ubiH::kan, ⌬ubiF::kan, and ⌬ubiE::kan mutations. The ⌬ubiI strain was cured to yield ⌬ubiIc which was then used to construct the ⌬ubiF⌬ubiI and ⌬ubiE⌬ubiI strains. Each mutation was transduced via P1 phage into MG1655. Muta-tions were confirmed using colony PCR with primers flanking the mutation.
S. cerevisiae was transformed using lithium acetate and was grown in YNB without pABA and folate (MP Biomedicals) with the indicated carbon sources (17).
Plasmid Construction-Plasmids are listed in supplemental Table S1, and their construction is detailed in the supplemental Materials and Methods.
Quinone Extraction and Quantification by HPLC-Electrochemical Detection (ECD) Analysis-5 ml of cells in exponential phase were centrifuged, and the pellet mass was determined. Glass beads (100 l), 50 l of 0.15 M KCl, and a volume of a Q solution (used as an internal standard) proportional to the wet weight were added to cell pellet. Q 10 was used for E. coli samples and Q 4 for S. cerevisiae samples. Lipids extraction was performed by adding 0.6 ml of methanol, vortexing for 10 min, then adding 0.4 ml of petroleum ether (boiling range 40 -60°C) and vortexing for 3 min. The phases were separated by centrifugation 1 min, 5000 rpm. The upper petroleum ether layer was transferred to a fresh tube. Petroleum ether (0.4 ml) was added to the glass beads and methanol-containing tube, and the extraction was repeated. The petroleum ether layers were combined and dried under nitrogen. The dried samples were stored at Ϫ20°C and were resuspended in 100 l of 98% ethanol, 20 mM lithium perchlorate. Aliquots were analyzed by reversedphase HPLC with a C18 column (Betabasic-18, 5 mm, 4.6 ϫ 150 mm; Thermo Scientific) at a flow rate of 1 ml/min using 40% ethanol, 40% acetonitrile, and 20% of a mix of 90% isopropyl alcohol and 10% lithium perchlorate (1 M) as a mobile phase. The mobile phase used to analyze yeast extracts was 5% isopropyl alcohol, 20% acetonitrile, and 75% of a mix of 98% methanol and 2% lithium perchlorate (1 M). Quinones were quantified with an ESA Coulochem III electrochemical detector and a 5011A analytical cell (E1, Ϫ650 mV; E2, 650 mV). Hydroquinones present in samples were oxidized with a precolumn 5020 guard cell set in oxidizing mode (E, ϩ650 mV). Different volumes of the standard Q solutions were injected in the same conditions to correct for sample loss during the organic extraction on the basis of the recovery of the Q internal standard. Standard curves for Q 4 and Q 10 were used for Q 6 and Q 8 quantification, respectively.
4-HP 8 Purification and Characterization-12 liters of LB culture of ⌬ubiI cells in stationary phase were centrifuged (4000 rpm, 15 min), and cell pellet mass was determined (30 g). Lipid extraction was performed for at least ten cycles at 50°C with 150 ml of acetone using a Soxhlet extractor. The extract was concentrated with a rotary evaporator, resuspended in chloro- form and applied on a silica column (M60; Macherey Nagel). Elution of molecules was realized with 100% of chloroform. Compounds of interest were collected in glass tubes, and 4-HP 8 was further purified by HPLC with 40% acetonitrile, 40% ethanol, and 20% of a mix of 90% isopropyl alcohol and 10% of water. Oxidized and reduced forms of 4-HP 8 were purified based on their characteristic UV spectra (see supplemental Fig. S3D). NMR spectra were recorded on a Bruker EMX 300 MHz spectrometer with D 6 -ethanol as solvent. Mass spectrometry measurements were carried out as described in the supplemental Materials and Methods.
Expression and Purification-Overexpression of UbiI was achieved by using BL21 (DE3) E. coli strain (Novagen) transformed with pET-ubiI-His 6 . The cultures were grown at 37°C until they reached A 600 ϭ 0.4 and then shifted to 20°C for induction. Isopropyl 1-thio-␤-D-galactopyranoside was added to 0.1 mM, and growth continued overnight. All subsequent operations were carried out at 4°C. Cells were harvested by centrifugation. The cell pellet was resuspended in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM DTT, 30% glycerol (v/v), and 1 mM Pefabloc (VWR). After sonication, the lysate was centrifuged at 180,000 ϫ g for 90 min. The resulting supernatant was loaded onto nickel-nitrilotriacetic acid Superflow resin (Qiagen) equilibrated with buffer 50 mM Tris-HCl, pH 7.5, 25 mM imidazole, 150 mM NaCl, 5 mM DTT, and 30% glycerol (v/v). UbiI was eluted with 250 mM imidazole, and after imidazole removal by ultrafiltration with Amicon Ultra 30K columns (Millipore), the purified protein was found to be Ͼ95% pure as judged by SDS-PAGE. The purified protein was aliquoted and stored at Ϫ80°C in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM DTT, and 30% glycerol (v/v). Selenomethionylated UbiI was overexpressed according to the indicated protocol (18) and purified with the same protocol as the native UbiI.
Limited Trypsin Proteolysis-To optimize the conditions of limited proteolysis, experiments were first conducted on an analytical scale (500 g of UbiI) where ratios of enzyme (trypsin) over substrate (UbiI) ranged from 1:15 to 1:1000 (w/w) in a final volume of 200 l. Digestion time was then optimized for a fixed enzyme over substrate ratio. Tryptic digestion analysis was performed by SDS-PAGE. Large scale preparation of cleaved UbiI was then carried out in 50 mM Tris-HCl, pH 7.5, 30% glycerol (v/v) at a protein concentration of 3 mg/ml. After 2 h of incubation of UbiI (21 mg) with trypsin (42 g) at 37°C, the reaction was stopped by the addition of Pefabloc (1 mM), followed by analytical SDS-PAGE. The N-terminal 40-kDa domain, which lacks the C-terminal His 6 tag, was purified on a nickel-nitrilotriacetic acid Superflow resin by collecting the flow-through and concentrated to 6 -8 mg/ml in 25 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10% (v/v) glycerol.
Protein Analysis-Mass spectrometry analysis (MALDI-TOF) was performed to delineate the C-terminal boundary of the N-terminal 40-kDa domain of UbiI, with the MASCOT software and Findpept tool (SiCaPS platform of IMAGIF (Gif-sur-Yvette, France). SEC-MALLS experiments were performed to determine the oligomeric state of the protein, in 25 mM Tris-HCl, pH 7.3, 150 mM NaCl, 10% (v/v) glycerol (IMAGIF Structural and Proteomic Biology Unit, Gif-sur-Yvette, France).
Data collection and structure determination were carried out as described in the supplemental Materials and Methods.
Docking Methods-A potential binding site of FAD in UbiI tr was explored using docking calculations. Comparing the binding site residues of FAD in PHBH (1PBE) with those in the crystal structure of UbiI tr , we noted that a number of residues are not favorably oriented to allow the docking of FAD. Preliminary to docking calculations, a UbiI tr monomer was thus prepared as follows: (i) Rotamers of Arg-49, Arg-35, and Asp-164 were selected to allow the one-step insertion of a rigid model of FAD in its extended conformation as observed in 1PBE. (ii) The binding site residues were further energy minimized using CHARMm in Discovery Studio 3.1. The resulting protein structure was then used as the receptor, and FAD was docked as a flexible ligand using Autodock software (19). The docking results place FAD in a range of plausible positions spanning the length of the binding pocket of UbiI tr . Among the best docked structures, we detail here a potential location of the FAD cofactor in UbiI selected for its highest similarity with the conformation observed in 1PBE. There is a rather good correspondence for ligand-receptor interactions between PHBH-FAD (1PBE) and the modeled UbiI tr -FAD complex (supplemental Table S4).

E. coli ⌬ubiI Synthesizes Low Q 8 Level in Aerobic Conditions-
We constructed a ⌬ubiI strain in which the ORF of ubiI has been replaced with a kanamycin resistance cassette. ⌬ubiI cells display a growth comparable with WT cells both on glucose and on succinate media (supplemental Fig. S2A) unlike ⌬ubiH cells, as observed previously (9). Evaluation of the cellular content in electroactive isoprenoid quinones by using ECD of lipid extracts after separation by HPLC (17,20) revealed that Q 8 content in ⌬ubiI cells accounted for only 7% of that in WT cells ( Fig. 2A). Furthermore, an unknown electroactive compound X could be detected (Fig. 2B). The levels of the isoprenoid naphthoquinones, demethylmenaquinone and menaquinone (MK 8 ), were not significantly different in WT and ⌬ubiI cells ( Fig. 2B and supplemental Fig. S2B). Transformation of ⌬ubiI cells with a plasmid containing the ubiI ORF restored Q 8 content and abolished the accumulation of compound X (Fig. 2, A  and B). ⌬ubiI cells grown anaerobically had a content of Q 8 comparable with that in WT cells (Fig. 2, C and D), and accumulation of compound X was abolished. As expected, a strong increase in MK 8 content was observed in cells grown anaerobically (21). Reversion of the Q 8 biosynthetic defect by anaerobic growth of ⌬ubiI cells is reminiscent of the phenotype of a ⌬ubiH strain (22) (Fig. 2, C and D). Collectively, these data establish that UbiI is involved in aerobic Q 8 biosynthesis but not in MK 8 biosynthesis. 8 -Compound X was purified from ⌬ubiI cells and found to be present in two redox states. Analysis by high resolution mass spectrometry in positive mode gave a m/z ratio (MϩH ϩ ) of 655.5445 for reduced compound X and 653.5297 for the oxidized form corresponding respectively to chemical formulas of C 46  652.5219; ppm, 0). Fragmentation of the 653.5 molecular ion yielded a characteristic tropylium-like ion (C 7 H 7 O 2 ϩ ), often found for aromatic compounds containing a benzyl unit, in addition to fragments corresponding to loss of methine, methylene, or methyl groups of an octaprenyl tail in the molecule (supplemental Fig. S3A). These data are consistent with compound X being a phenyl ring functionalized with an octaprenyl tail and either two OH groups in the reduced (quinol) C 46 H 70 O 2 molecule or two keto groups in the oxidized (quinone) C 46 H 68 O 2 molecule (supplemental Fig. S3B). The 1 H NMR spectrum of the purified C 46 H 70 O 2 compound shows well defined peaks both in the aromatic and aliphatic regions (supplemental Fig. S3C). Most of the signals in the 3.2-5.3 ppm region could be attributed to the vinylic and allylic protons of the octaprenyl substituent. The aromatic region of the spectrum shows more relevant information. Three distinguishable massifs, each corresponding to a unique proton, can be observed at 6.44 ppm (doublet of doublet with 4 J and 3 J coupling constants), 6.57 ppm (doublet with a 4 J coupling constant), and 6.61 ppm (doublet with a 3 J coupling constant) (Fig. 2E). These results confirm a 1,2,4-trisubstitution of the phenyl ring. The NMR spectrum of the commercially available 2-methyl-4hydroxyphenol shows a highly similar pattern with close chemical shifts and coupling constants (Fig. 2E). The NMR data thus establish that the two hydroxyl groups of the C 46 H 70 O 2 compound are in para relative position. Accordingly, the UV spectra of oxidized and reduced forms of compound X and of 2-methyl-4-hydroxyphenol are comparable (supplemental Fig. S3, D and E). Collectively, all of these data identify 4-HP 8 (Fig. 2F) as the compound that accumulates in ⌬ubiI cells.

E. coli ⌬ubiI Accumulates 4-HP
UbiI Complements a C5-hydroxylation Defect in S. cerevisiae-Drastic decrease of Q 8 and accumulation of 4-HP 8 , lacking a OH or a OCH 3 group at C5 are strong indications that the C5-hydroxylation reaction is deficient in E. coli ⌬ubiI cells and that UbiI functions as a C5-hydroxylase in Q 8 biosynthesis. To verify this hypothesis, we tested whether heterologous expression of UbiI was able to complement a S. cerevisiae ⌬coq6 strain which lacks the C5-hydroxylase Coq6 (17). In combination with Coq8 overexpression, required for maintaining the integrity of the Q biosynthetic complex in S. cerevisiae (23), the mitochondria-targeted E. coli UbiI protein was found to partially restore respiratory growth of ⌬coq6 cells on lactate-glycerol medium, a process that requires coenzyme Q (Fig. 3A). HPLC-ECD analysis of cellular lipid extracts of ⌬coq6/Coq8ϩUbiI cells demonstrated the presence of Q 6 together with 4-HP 6 ( Fig.  3B) whereas ⌬coq6/Coq8 cells accumulated exclusively 4-HP 6 (17). Complementation by UbiI is not optimal but is nevertheless significant because ⌬coq6/Coq8ϩUbiI cells contain approximately 12% of the amount of Q 6 present in ⌬coq6/Coq6 cells (Fig. 3C). These results establish that UbiI can function as a C5-hydroxylase in S. cerevisiae.
The residual C5-hydroxylase Activity in ⌬ubiI Cells Is Provided by UbiF-⌬ubiI cells synthesize a small amount of Q 8 ( Fig. 2A), reflecting that the C5-hydroxylation activity, although limited, is still taking place. To test the involvement of genes of the Q pathway in this biosynthetic activity, we constructed ⌬ubiF ⌬ubiI and ⌬ubiE ⌬ubiI mutants. ⌬ubiE cells accumulate the biosynthetic intermediate C2-demethyl-C6demethoxy-Q 8 (DDMQ 8, peak at 6.87 min in Fig. 4A), as published before (7). Deletion of ubiI in a ⌬ubiE strain led to the accumulation of 4-HP 8 and of a diminished amount of DDMQ 8 (Fig. 4A). In contrast, deletion of ubiI in the ⌬ubiF strain caused the expected accumulation of 4-HP 8 but also the complete disappearance of DMQ 8 (Fig. 4B), showing that the C5-hydroxylation reaction is totally abolished in the ⌬ubiF ⌬ubiI strain. Collectively, these results demonstrate that the residual C5-hydroxylase activity observed in ⌬ubiI cells is provided by UbiF, the C6-monooxygenase.

Crystal Structure of a C-terminal Truncated Form of UbiI-
We overexpressed and purified the UbiI-His 6 protein which contains a C-terminal His 6 tag. However, the protein was cofactorfree, as shown by UV-visible spectroscopy, and highly unstable, with a significant propensity to aggregate and precipitate. Limited trypsin proteolysis of UbiI-His 6 generated a soluble fragment (molecular mass ϳ40 kDa, supplemental Fig. S4A). MALDI-TOF mass spectrometry of the UbiI tr (supplemental Fig. S4B) together with the result that UbiI tr does not bind to nickel-nitrilotriacetic acid, suggested that ϳ35 residues at the C terminus had been removed (two cleavage sites after residue 364 and 365, respectively), as confirmed below by the crystal structure of UbiI tr . The truncated UbiI tr protein expressed from a plasmid was not able to complement the ⌬ubiI strain because the quinone profile was similar to that of ⌬ubiI cells containing an empty vector (supplemental Fig. S4C). UbiI tr behaved as a tetramer in solution, as shown by SEC-MALLS experiments (supplemental Fig. S4D), and crystallized in space group P2 1 2 1 2.
The structure of the native UbiI tr protein was solved at 2.8 Å resolution by the single anomalous dispersion method using the selenomethionylated protein and refined to 2.0 Å resolution (supplemental Table S2 and supplemental Fig. S5). The overall tertiary structure of the tetramer reveals that each monomer contains a typical FAD-binding domain with a Rossman-like ␤/␣/␤-fold (Fig. 5A). A search for proteins with similar fold with DALI (24) indicates that the structure of UbiI tr is similar to that of several flavin-dependent monooxygenases, including PHBH) from Pseudomonas fluorescens (supplemental Table  S3). Superimposition of the structure of UbiI tr with that of PHBH in complex with FAD (Fig. 5B) supports the presence of a FAD binding pocket in UbiI tr .
The FAD Binding Site in UbiI tr -The existence of a binding site for a FAD cofactor in UbiI was confirmed with docking experiments and minimization simulations using a UbiI tr monomer and the extended conformation of FAD from PHBH (1PBE) as the starting position (25). The results of these calculations are shown in Fig. 5C. The docked FAD in UbiI tr exhibits a number of canonical interactions reminiscent of those involving FAD in PHBH, especially at the isoalloxazine ring and the ribityl chain ( Fig. 5C and supplemental Table S4). Fig. 5C shows that the isoalloxazine ring is located within hydrogen bonding distances of the main-chain nitrogens of Leu-297, Val-302, Asn-303, and Ala-52, and the side-chain amide nitrogen atom of Asn-303. Also, it may be stabilized by -stacking with Phe-269, an interaction shared in a number of other flavoproteins (26 -28). The ribityl chain is hydrogenbonded to side chains of Arg-49 and Asn-110, as it is in PHBH using Arg-44 and Gln-102. Finally, the negatively charged pyrophosphate group is electrostatically complemented by: (i) the ␣1 helix dipole of the ␤/␣/␤-fold at the N terminus as in PHBH; (ii) hydrogen bonding with the side chain of Arg-49 residue (Arg-44 in PHBH) and the main chain of Met-13, a residue within the conserved GXGXXG motif of flavoproteins (Ser-13 in PHBH).
Our model of the docked FAD in UbiI tr was validated by site-directed mutagenesis. We indeed mutated both residues Gly-301 and Asn-303, which are conserved in flavoprotein monooxygenases (29) (supplemental Fig. S1). These residues form the bottom of the isoalloxazine binding pocket in UbiI tr . Expression of UbiI-G301A-N303D in the ⌬ubiI strain led to a Q 8 /4-HP 8 ratio comparable with that found in ⌬ubiI cells containing an empty vector (Fig. 5D). In contrast, expression of UbiI led to the complete disappearance of 4-HP 8 (Fig. 2B). These results show that the G301A/N303D double mutation strongly impairs UbiI activity probably as the consequence of a  steric effect from the G301A mutation and the substitution of a hydrogen-bond stabilization by a repulsive electrostatic interaction resulting from the N303D mutation, both predicted to substantially affect the binding of FAD.

DISCUSSION
By using a combination of genetic, biochemical, and structural approaches, we have established that visC, a gene of unknown function in E. coli, encodes a protein that plays an important role in coenzyme Q biosynthesis under aerobic conditions. Consequently, we have renamed this gene ubiI. We show here that the UbiI protein functions in the C5-hydroxylation reaction of aerobic Q biosynthesis in E. coli.
More precisely, the function of the UbiI protein is to introduce the OH group on the C5 carbon of the phenyl ring of ubiquinone as supported by several lines of evidence. First, 4-HP 8 that accumulates in ⌬ubiI cells is diagnostic of a deficiency in the C5-hydroxylation reaction. Indeed, 4-HP 8 results from decarboxylation of OHB (reaction b, Fig. 1) to form OPP, which is then hydroxylated at position C1 (reaction e) without C5-hydroxylation (reaction c) and O5-methylation (reaction d) taking place. Likewise, we have shown that S. cerevisiae strains deficient for the C5-hydroxylation accumulate 4-HP 6 (17,20). Second, UbiI complements the C5-hydroxylation defect of ⌬coq6 S. cerevisiae cells (Fig. 3), which shows that UbiI is a functional ortholog of the C5-hydroxylase Coq6. Given the modest sequence identity between UbiI and Coq6 (25%) and the fact that Coq proteins are organized in a multiprotein complex (3), it is not surprising that UbiI does not fully complement the deletion of coq6 (Fig. 3C). Third, we have established that  Table S4. D, corrected integration of the electrochemical signal for the peaks corresponding to Q 8 (in black) and 4-HP 8 (in gray) after HPLC analysis of lipid extracts from ⌬ubiI cells containing an empty vector, pBAD-ubiI, or pBAD-ubiI(G301A/N303D) (n ϭ 4). The ratio of the area of the Q 8 and 4-HP 8 peaks is indicated.
UbiI displays a number of characteristics of FAD-dependent monooxygenases: (i) it shows high sequence homology with members of this class of enzymes, including the well studied PHBH and also UbiH, UbiF, and Coq6 (supplemental Fig. S1); (ii) the crystal structure of UbiI tr shows great structural homology with PHBH, including at the level of a binding pocket for a FAD cofactor, as confirmed by docking experiments; (iii) Gly-301 and Asn-303, which contribute to the FAD binding pocket, are important for the C5-hydroxylase activity of UbiI. On the basis of these results, we propose that UbiI is a FAD-dependent monooxygenase that catalyzes the O 2 -dependent C5-hydroxylation reaction of coenzyme Q 8 biosynthesis in aerobic conditions.
It should be noted that very few Ubi proteins involved in Q 8 ring modification have been characterized biochemically, maybe as a result of limited stability and solubility when these proteins are expressed individually, as encountered with UbiI. In fact, only the purified O-methyltransferase UbiG has been shown to display in vitro activity so far (6). In the case of UbiI, we had to work with a truncated form lacking 34 amino acids in the C terminus to obtain a homogeneous protein in solution that we could crystallize. Unfortunately, this protein was inactive when assayed in vivo and could not bind FAD in vitro. Further studies are required to obtain a pure active UbiI enzyme. Nevertheless, our structure of UbiI is the first x-ray crystal structure of a monooxygenase implicated in Q biosynthesis.
A fascinating question raised by our work concerns the role of UbiB in Q biosynthesis. We clearly show that UbiI is responsible for the C5-hydroxylation reaction, and therefore UbiB is not catalyzing this step as this has been thought for years (4). An attractive possibility is that UbiB functions in regulating Q 8 biosynthesis via its putative kinase activity as originally proposed by Clarke and co-workers (12). If so, the function of UbiB would be consistent with the emerging function of its eukaryotic homologs from S. cerevisiae (Coq8) (23) and humans (ACDK3/CABC1) (30).
Our characterization of ubiI yielded intriguing observations regarding the hydroxylation reactions. First, inactivation of ubiI does not completely abrogate Q 8 biosynthesis. We have shown that in the absence of UbiI, the C6 monooxygenase UbiF is able to hydroxylate the neighboring C5, which points to a relatively poor regioselectivity of UbiF. However, there is no doubt that the main C5-hydroxylase is UbiI and not UbiF because UbiF only promotes the biosynthesis of 7% of WT Q 8 levels in ⌬ubiI cells and because the C5-hydroxylation is not deficient in ⌬ubiF cells because only DMQ 8 but no 4-HP 8 is detected in these cells. Second, we have shown that UbiI is dispensable for anaerobic Q 8 biosynthesis similarly to what was reported for UbiH and UbiF (22). As dioxygen is the source of the three oxygen atoms that are incorporated during the aerobic hydroxylation reactions (reactions c, e, and g, Fig. 1) of Q 8 biosynthesis (31), the flavin-dependent monooxygenases UbiF, UbiH, and UbiI do not function in anaerobic conditions. Therefore, an alternative unidentified enzymatic system must substitute for UbiI, UbiF, and UbiH to catalyze the hydroxylation reactions during anaerobic Q 8 biosynthesis. The next challenge will be to identify the anaerobic hydroxylation system.