Identification of Cysteine Residues in Human Cationic Amino Acid Transporter hCAT-2A That Are Targets for Inhibition by N-Ethylmaleimide

Background: The mechanism of N-ethylmaleimide (NEM)-mediated inhibition of cationic amino acid transporters (CATs) was unknown. Results: Cys-33 (cytoplasmic N terminus) and Cys-273 (transmembrane domain VI) in human CAT-2A are the targets of NEM inhibition. Conclusion: The cytoplasmic N terminus and transmembrane domain VI are critically involved in transporter function. Significance: Our results help to understand how these important amino acid transporters are regulated, probably also in vivo. In most cells, cationic amino acids such as l-arginine, l-lysine, and l-ornithine are transported by cationic (CAT) and y+L (y+LAT) amino acid transporters. In human erythrocytes, the cysteine-modifying agent N-ethylmaleimide (NEM) has been shown to inhibit system y+ (most likely CAT-1), but not system y+L (Devés, R., Angelo, S., and Chávez, P. (1993) J. Physiol. 468, 753–766). We thus wondered if sensitivity to NEM distinguishes generally all CAT and y+LAT isoforms. Transport assays in Xenopus laevis oocytes established that indeed all human CATs (including the low affinity hCAT-2A), but neither y+LAT isoform, are inhibited by NEM. hCAT-2A inhibition was not due to reduced transporter expression in the plasma membrane, indicating that NEM reduces the intrinsic transporter activity. Individual mutation of each of the seven cysteine residues conserved in all CAT isoforms did not lead to NEM insensitivity of hCAT-2A. However, a cysteine-less mutant was no longer inhibited by NEM, suggesting that inhibition occurs through modification of more than one cysteine in hCAT-2A. Indeed, also the double mutant C33A/C273A was insensitive to NEM inhibition, whereas reintroduction of a cysteine at either position 33 or 273 in the cysteine-less mutant led to NEM sensitivity. We thus identified Cys-33 and Cys-273 in hCAT-2A as the targets of NEM inhibition. In addition, all proteins with Cys-33 mutations showed a pronounced reduction in transport activity, suggesting that, surprisingly, this residue, located in the cytoplasmic N terminus, is important for transporter function.

Most cell types express at least two different transport systems for cationic amino acids (CAAs) 3 such as L-arginine, L-lysine, and L-ornithine. System y ϩ was first described in Ehrlich cells as system Ly ϩ (to refer to the positively charged substrate lysine) and was later renamed system y ϩ when it became evident that other CAAs share the same transport system (1,2). It works independently of the presence of Na ϩ and H ϩ ions, exhibits a relatively high affinity for CAAs, and works preferentially as an exchanger of CAAs, evidenced by its strong trans-stimulation. The second system, named y ϩ L, was first described in erythrocytes (3). It works as an obligatory exchanger of CAAs and neutral amino acids, with transport of CAAs being Na ϩ -independent (like system y ϩ ). However, transport of neutral amino acids by system y ϩ L occurs in a Na ϩ -dependent manner.
Working with human erythrocytes, Devés et al. (4) demonstrated that system y ϩ is inhibited by short-term treatment with the thiol-modifying agent N-ethylmaleimide (NEM; 0.2 mM, 10 min). In contrast, system y ϩ L, also present in these cells, is insensitive to NEM. Later, molecular identification of amino acid transporters revealed that there are several isoforms of SLC7 (solute carrier family 7) (see Fig. 1A) that mediate either system y ϩ (and y ϩ -like) or y ϩ L activity. Whether sensitivity to NEM generally distinguishes all system y ϩ (and y ϩ -like) from system y ϩ L isoforms had not been elucidated so far.
Most members of the SLC7 branch of cationic amino acid transporters (CATs) exhibit transport properties similar to system y ϩ . All CAT proteins transport exclusively CAAs in a Na ϩindependent manner. They are glycosylated and predicted to have 14 transmembrane domains (TMDs) (5,6). The transport properties of CAT-1 (SLC7A1) conform most with the transport system described in Ehrlich cells (7). CAT-2B (high affinity SLC7A2) and CAT-3 (SLC7A3) are less dependent on substrate at the trans-side of the membrane, and both CAT-2 splice variants (A and B) exhibit diminished activity at low pH (7)(8)(9)(10). CAT-2B and CAT-3 can thus be seen as transporters resembling system y ϩ and are referred to here as y ϩ -like. Finally, CAT-2A is not at all trans-stimulated and has a low substrate affinity (11,12). It is highly expressed in the liver, the only organ found so far to lack system y ϩ activity. Hence, although clearly a member of the CAT family, CAT-2A does not qualify as a system y ϩ transporter.
The second branch of the SLC7 family consists of the socalled light chains of heteromeric amino acid transporters (lcHATs), which are not glycosylated and are predicted to have 12 TMDs (13). In contrast to the CATs, lcHATs need to associate with glycoproteins to localize to the plasma membrane. When coexpressed with the glycoprotein 4F2hc, the lcHATs y ϩ LAT1 (SLC7A7) and y ϩ LAT2 (SLC7A6) mediate system y ϩ L activity (14,15).
NEM is frequently used to inhibit enzymes with cysteine residues in their active center. In a nucleophilic attack, it forms a thioether with the sulfhydryl group of cysteine residues. This reaction is specific in a pH range of 6.5-7.5. At lower pH, NEM may also react with histidine (16). Our previous work with human transporters expressed in Xenopus laevis oocytes already demonstrated that NEM (at concentrations of 0.2 and 0.5 mM) inhibits CAT-1 but leaves y ϩ LAT2/4F2hc unaffected (17). The aim of the present study was to determine (i) whether CATs and y ϩ LATs can generally be distinguished by their reactivity with NEM and (ii) which cysteine residues within CAT proteins are responsible for sensitivity to NEM.

EXPERIMENTAL PROCEDURES
Site-directed Mutagenesis-This was performed using the QuikChange mutagenesis kit (Stratagene). The sequence of each oligonucleotide pair used is presented in Table 1.
Expression of cRNA in X. laevis Oocytes-The plasmids were linearized and, in the case of 3Ј-overhangs (restriction with SacI), blunted using the Klenow fragment of Escherichia coli DNA polymerase I. cRNA was prepared by in vitro transcription from the SP6 promoter (Ambion mMESSAGE mMACHINE in vitro transcription kit, AMS Biotechnology (Europe)Ltd., Cambridgeshire, United Kingdom). 18 ng of cRNA in 36 nl of water were injected in each X. laevis oocyte (Dumont stages V and VI). Non-injected oocytes were used as controls. If not indicated otherwise, all experiments were performed 2 days after cRNA injection.
Inhibition Experiments with NEM-The oocytes were first rinsed three times with ice-cold uptake solution (100 mM NaCl, 2 mM KCl, 1 mM MgCl 2 , 1 mM CaCl 2 , 5 mM HEPES, and 5 mM Tris, pH 6.8) and then transferred to the same solution with the indicated NEM concentration (Sigma-Aldrich) and incubated for 10 min at 20°C. The same amount of uptake solution supplemented with L-[ 3 H]arginine (ICN Biomedicals GmbH, Eschwege, Germany) was added at a final concentration (if not indicated otherwise) of 1 mM (10 Ci/ml). After incubation for 15 min at 20°C, oocytes were washed five times with ice-cold uptake solution and solubilized individually in 2% SDS. The radioactivity of the lysates was determined in a liquid scintillation counter.
Determination of Apparent K m and V max Values-Oocytes were washed three times with ice-cold uptake solution (pH 6.8) and then equilibrated in the same solution supplemented with 0.1-10 mM unlabeled L-arginine at 18°C. The oocytes were transferred to the same solution containing, in addition, L-[ 3 H]arginine (10 Ci/ml). After a 15-min incubation at 20°C, oocytes were washed and processed as described above.
Statistical Analysis-Statistical analysis was performed using one-way analysis of variance (ANOVA), followed by the Bonferroni post hoc test. If only two groups were compared, the t test was used. The confidence interval was always 95%.

RESULTS
hCATs, but Not y ϩ LATs, Are Inhibited by NEM-To examine whether all validated hCAT proteins are inhibited by NEM and the two y ϩ L isoforms are resistant to the SH group-modifying agent, we analyzed arginine transport by the individual transporters expressed in oocytes from X. laevis in the presence and absence of 1 mM NEM (Fig. 1B). For transport studies, 1 mM arginine was used in experiments with low affinity hCAT-2A, 0.1 mM arginine was used in experiments with all other transporters. We found that NEM inhibited not only the molecular representative of system y ϩ (hCAT-1) but also the system y ϩ -like transporters hCAT-2B and hCAT-3 and the non-system y ϩ transporter hCAT-2A. In each case, a t test yielded p Ͻ 0.001 when comparing values from NEM-treated oocytes and untreated controls. NEM inhibition of the CAT proteins was quite pronounced, leaving only 16% (hCAT-1), 1% (hCAT-2A), 7% (hCAT-2B), and 16% (hCAT-3) residual activity compared with untreated oocytes expressing the same transporter. In contrast, neither molecular representative of system y ϩ L, y ϩ LAT1 or y ϩ LAT2 (each time coexpressed with 4F2hc). FIGURE 1. NEM inhibits transport activity of hCATs, but not y ؉ LATs. A, the phylogenetic tree shows the known members of the SLC7 family of amino acid transporters (AT). CATs are glycosylated, predicted to have 14 TMDs, and mediate exclusively the transport of CAAs. A4 and A14 are glycosylated orphan proteins also predicted to have 14 TMDs. lcHATs are not glycosylated and are predicted to have 12 TMDs. To traffic to the plasma membrane, they need to associate with a glycoprotein (depending on the isoform: 4F2hc, rBAT, or a still unidentified protein). B, X. laevis oocytes overexpressing the indicated y ϩ (hCAT-1) and y ϩ -like (hCAT-2B or hCAT-3) transporters, hCAT-2A, or y ϩ L transporters (y ϩ LAT1 or y ϩ LAT2/4F2hc) were preincubated in buffer with or without 1 mM NEM for 10 min, followed by incubation in buffer containing L-[ 3 H]arginine (1 mM for hCAT-2A and 100 M for all others) for an additional 15 min. After intensive washing, the radioactivity per oocyte was determined, and the values of non-overexpressing oocytes were subtracted. Values are expressed as a percentage of the mean of the respective control (not treated with NEM). Bars represent means Ϯ S.E. (n Ն 8 from at least two different batches of oocytes). Statistical analysis between NEM-treated und untreated oocytes expressing the same transporter was performed using a t test. ***, p Յ 0.001; no labeling corresponds to p Ͼ 0.05. NEM Does Not Influence the Subcellular Distribution of hCAT-2A-To test whether the NEM-mediated reduction of CAT-mediated transport is conveyed by internalization of transport proteins, we examined cryosections of hCAT-2A-EGFP-expressing oocytes exposed for 10 min immediately before freezing to 1 mM NEM or to buffer only. By far the largest part of the fluorescent signal was found in the oocyte plasma membrane under both conditions ( Fig. 2A), indicating that NEM treatment causes no change in the localization of the transporter. To verify this result, cell surface proteins of intact hCAT-2A-expressing oocytes preincubated with 1 mM NEM for 10 min or untreated were biotinylated. Subsequent isolation of the biotinylated proteins and analysis by Western blotting revealed no change in the cell surface localization of hCAT-2A upon NEM treatment (Fig. 2B). This indicates that NEM inhibits the intrinsic hCAT activity.
Identification of Cysteine Residues in hCAT-2A That Mediate NEM Inhibition-We next wondered if NEM causes hCAT inhibition by directly modifying sulfhydryl groups in cysteine residues of the transporters. To answer this question, we focused on hCAT-2A because it has a relatively small number of cysteine residues among the hCATs, exhibits a robust transport activity in X. laevis oocytes, and showed the most pronounced inhibition by NEM of all hCATs.
Because all hCAT proteins were inhibited by NEM, we reasoned that NEM should act on cysteine residue(s) conserved among the hCATs. Cys-33, Cys-85, Cys-264, Cys-273, Cys-299, Cys-347, and Cys-427 were found to meet this condition. Of these, only Cys-85 is also found at corresponding positions in y ϩ LAT1 and y ϩ LAT2, which are not inhibited by NEM. All seven cysteine residues in hCAT-2A were substituted individually with alanine by site-directed mutagenesis. Of the resulting seven single mutants, four exhibited transport activity comparable to WT in uptake studies using 1 mM L-[ 3 H]arginine (Fig.  3). One mutant had a higher activity and two mutants had lower activities compared with WT. Transport measurements at different arginine concentrations revealed that, compared with WT, the C33A and C264A mutants exhibited a reduced and an increased V max value, respectively (1.7 Ϯ 0.3 (C33A), 10.7 Ϯ 0.3 (C264A), and 6.9 Ϯ 0.7 (WT) nmol/oocyte/h; one-way ANOVA, followed by the Bonferroni post hoc test, p Ͻ 0.001 in each case). Neither total expression nor cell surface expression of the C33A mutant was different compared with WT (Fig. 3B). The apparent K m of hCAT-2A-C33A was even lower than that of WT (1.3 Ϯ 0.1 versus 3.0 Ϯ 0.2 mM, p Ͻ 0.05), whereas the apparent K m of hCAT-2A-C264A (2.6 Ϯ 0.6 mM) was not significantly altered.
Surprisingly, all single cysteine mutants were still inhibited by NEM (Fig. 3A). To determine whether NEM affects any of the cysteine residues in hCAT-2A, we constructed a transporter devoid of cysteine residues (cysteine-less (CL), hCAT-2A-CL) by mutating all 13 cysteine residues within hCAT-2A to alanine. This mutant had only ϳ8% transport activity compared with WT after 2 days of expression in X. laevis oocytes (data not shown). Upon examining the total and cell surface expression of hCAT-2A-CL, it became evident that both were much lower for hCAT-2A-CL than for WT, whereas the ratio of total to surface protein was comparable to that of WT (Fig. 4A). Because of low protein expression and transport activity, the expression time was extended from 2 to 4 days, resulting in a higher amount of protein detected in Western blot analysis (Fig. 4B) and an ϳ25% transport activity compared with WT. Inhibition experiments revealed that the cysteine-less mutant was not inhibited at any NEM concentration used (0.2, 0.5, or 1 mM) (Fig. 4C). Even a t test comparing oocytes treated with 1 mM NEM and untreated controls yielded a p value of 0.25.
The resistance of the cysteine-less hCAT-2A protein to inhibition by NEM strongly suggested that cysteine residues within hCAT-2A were conveying the NEM inhibition of WT. As individual mutation of each of the conserved cysteine residues was not sufficient to abolish NEM inhibition (Fig. 3A), it seemed likely that more than one cysteine residue may mediate the NEM effect. We therefore had a closer look at the results in Fig.  3A and noticed that, in contrast to WT and all other mutants, (i) the C33A and C85A mutants exhibited no tendency for inhibition at the lowest NEM concentration used (0.2 mM), and (ii) the C85A and C273A mutants were not completely inhibited by NEM at 1 mM. We therefore created double mutants, each carrying alanine mutations at two of these three cysteine residues. In fact, the C33A/C273A mutant was not inhibited at any NEM concentration used (Fig. 5). A t test comparing oocytes treated with 1 mM NEM and controls yielded a p value of 0.43. In contrast, the double mutants C33A/C85A and C85A/C273A were still significantly inhibited by NEM.
These data strongly suggest that NEM inhibition occurs at Cys-33 and Cys-273. In fact, a mutant in which all cysteine residues except Cys-33 and Cys-273 were mutated to alanine (hCAT-2A-CL(C33ϩ273)) was inhibited by NEM (Fig. 6A). In addition, it exhibited a much higher transport activity compared with hCAT-2A-CL. Because the two single cysteine mutants hCAT-2A-C33A and hCAT-2A-C273A were (almost) as sensitive to NEM as WT, we concluded that modification of one of these two cysteine residues by NEM would be sufficient to inhibit hCAT-2A. Indeed, mutants in which all cysteine residues except Cys-33 or Cys-273 were mutated to alanine, hCAT-2A-CL(C33) and hCAT-2A-CL(C273), were still inhibited by NEM (Fig. 6, B and C). In addition, transport of hCAT-2A-CL(C33) was about three times higher than that of hCAT-2A-CL (data not shown). This was not due to increased total or cell surface expression compared with hCAT-2A-CL. In contrast to hCAT-2A-CL(C33), transport of hCAT-2A-CL(C273) was comparable to hCAT-2A-CL: even after 4 days of expres-

DISCUSSION
NEM Inhibits CATs, but Not y ϩ LATs-Our study has established that all CAT proteins, but neither of the y ϩ LAT isoforms, are inhibited by the cysteine-modifying compound NEM. Hence, NEM can be used to generally distinguish transport of CAAs by CATs and y ϩ LATs. They belong to two different branches of the SLC7 family and are often coexpressed in mammalian cells. However, NEM does not distinguish between transporters with system y ϩ (CAT-1), y ϩ -like (CAT-2B and CAT-3), and non-y ϩ (CAT-2A) activity within the CAT branch. In line with previous results (4), NEM inhibition occurred rapidly. A closer analysis of hCAT-2A showed that inhibition was not due to protein degradation or a change in plasma membrane localization of the transporter. Hence, NEM most likely affects the intrinsic activity of the CATs.

NEM Inhibition Occurs at Residues 33 and 273 in hCAT-2A-Mutational analysis of hCAT-2A further demonstrated that NEM inhibits CAT activity directly via interaction with Cys-33
and Cys-273, conserved in CATs, but not y ϩ LATs. The presence of one of these residues is sufficient to confer NEM inhibition as evidenced by the observations that (i) simultaneous mutation of both residues, but not of either one alone, conferred NEM resistance to hCAT-2A, and (ii) hCAT-2A mutants in which all cysteine residues except Cys-33 and/or Cys-273 were mutated to alanine were sensitive to NEM. This strongly suggests that NEM inhibition of CATs is mediated by alkylation of either Cys-33 and Cys-273 in hCAT-2A.
Neither Cys-33 nor Cys-273 of hCAT-2A is conserved in other non-CAT SLC7 family members (lcHATs), suggesting that none of them are inhibited by NEM. One exception is SLC7A13, which carries a cysteine at the position corresponding to Cys-273 in hCAT-2A. Together with a mouse homolog (Asc-2 or XAT1), this transporter builds an additional branch of the SLC7 family (Fig. 1A) whose members are proposed to associate with yet unknown heavy chain(s) to localize to the plasma membrane (29 -31). SLC7A13 mediates aspartate/glutamate exchange when covalently linked to either 4F2hc or rBAT (31). It would be interesting to see if it is inhibited by NEM.
Based on the models predicting 14 TMDs for the CATs, Cys-33 localizes to the intracellular N terminus, whereas Cys-273 resides at the cytoplasmic side of TMD VI. For a better prediction of the position of the two cysteine residues within hCAT-2A, we performed homology modeling using known crystal structures of bacterial transporters found to be related to hCAT-2A (ApcT, AdiC, and LeuT) (Fig. 7). According to the resulting model, both Cys residues localize far away from the putative substrate-binding site, suggesting that transporter modification by NEM does not interfere with substrate binding. This is consistent with the notion that the presence of substrate does not protect system y ϩ against inhibition by NEM (4). Further evidence against an involvement of either Cys-33 or Cys-273 in substrate binding comes from transport studies: the hCAT-2A mutant in which Cys-273 was mutated to Ala exhibited undiminished transport activity, and the C33A mutant (even though exhibiting less transport activity) did not have a reduced apparent substrate affinity. Alkylation by NEM of either cysteine residue in hCAT-2A must thus inhibit other essential steps in the transport process. It may simply result in a steric hindrance for the substrate to access its binding site. Alternatively, it may interfere with conformational changes necessary for the transport process. Also, the mechanism of NEM inhibition may not be the same at the two different sites.
Interestingly, Cys-31, located just one residue away from Cys-33 in hCAT-2A, does not seem to be important to NEM inhibition because the C33A/C273A mutant (containing Cys-31) was not inhibited by NEM, and the mutants containing exclusively Cys-33 and/or Cys-273 (but not Cys-31 or any other Cys residue) were fully inhibited. It seems likely that this residue is also alkylated by NEM because it should be at the wateraccessible surface and thus reactive with the thiol reagent. Both residues are adjacent to a basic residue (Arg-32), favoring the reaction with NEM (32). Cys-31 and Cys-33 form a CXC motif, found in proteins involved in isomerization of disulfide bridges (33). However, as Cys-31 is not conserved among CATs, this motif does not seem to play a role in either CAT function or inhibition by NEM. If modification of Cys-31 has no impact on hCAT-2A activity, it seems unlikely that NEM inhibition through Cys-33 is simply due to steric hindrance, but rather that alkylation by NEM leads to loss of Cys-33 function in hCAT-2A (see below).
Importance of Cys-33, but Not Cys-273, for hCAT-2A Activity-In line with an important function of Cys-33 for hCAT-2A activity is the observation that mutation of this residue to alanine diminished hCAT-2A activity considerably. The reduced transport activity was not due to diminished protein expression, localization of the transporter to the plasma membrane (Fig. 3B), or reduced substrate affinity; the apparent K m of the Cys-33 mutant was even decreased. Also, substituting alanine with serine did not improve the transport activity of the single (hCAT-2A-C33S) or double (hCAT-2A-C33S/C273S) mutant (data not shown). The single mutant in which Cys-33 was replaced with methionine (hCAT-2A-C33M) exhibited even a markedly lower activity than the C33A mutant (data not shown). This suggests that a cysteine at position 33 is important for high transport capacity. In line with this view is the largely improved transport activity of the cysteine-less hCAT-2A mutant after reintroduction of Cys-33. Again, this increase was not accompanied by an increase in protein expression (data not shown).
The cysteine-less mutant exhibited significant (although low) transport activity, which was resistant to NEM inhibition. The activity was even lower than that of the single mutant C33A. However, this seemed to be due mainly to poor expression of the protein, most likely because of diminished protein stability (and not diminished translation). The low activity and stability of the cysteine-less mutant are the result of the different effects of each individual Cys mutation. Among the conserved cysteine residues, only the mutation of Cys-427 to alanine also reduced hCAT-2A transport activity, but to a lesser extent than that of Cys-33. In contrast, the hCAT-2A-C264A mutant had an even higher transport activity than WT. The effects of mutating the non-conserved cysteine residues were not investigated for each individual residue. However, the hCAT-2A-C4A/C13A/C31A mutant, in which all cysteine residues in the N terminus with the exception of Cys-33 were mutated, had a transport activity comparable to WT (data not shown), suggesting that these residues have no special function in the transporter. This excludes an essential interaction of Cys-33 with any of these residues (including the CXC motif mentioned above) and further speaks for a specific function of Cys-33.
Cys-33 seems to have an important function in CAT-mediated transport. This is surprising given that it is predicted to localize to the cytoplasmic N terminus and thus is unlikely to participate in substrate binding or transition. However, the crystal structures of related proteins available so far do not allow any structural predictions in the N-terminal region of hCATs. We can therefore not exclude that Cys-33 might be part of the transition pore.
Interestingly, NEM inhibition of selenium export by the anion exchanger AE1 seems also to be mediated by cysteine residues within the cytoplasmic N terminus of the transporter (34). The specific function of these N-terminal cysteine residues needs to be elucidated. As mentioned above, Cys-33 is not conserved in any of the lcHATs. Given that these proteins all function as amino acid transporters, with some of them also recognizing CAAs as their substrate, it is puzzling to predict the specific role Cys-33 may have in CAT function.
A similar situation is seen in mitochondrial phosphate transporters: whereas the mammalian isoform contains a Cys residue essential for transporter function and inhibition by NEM, the yeast isoform does not (32). However, the amino acid residue at the corresponding position in the yeast transporter (Thr) is also essential for high transport capacity (35). It is thus tempt-   OCTOBER 18, 2013 • VOLUME 288 • NUMBER 42 ing to speculate that the glutamic acid residue found in both y ϩ LAT isoforms and three other lcHATs at the position corresponding to Cys-33 in hCAT-2A is also essential for transporter function.

Cysteine Residues in hCAT-2A Targeted by NEM
Could a Modification of Either Cys-33 or Cys-273 Also Play a Role in the Regulation of hCAT Activity in Living Cells?-As for now, we can only speculate if the target residues for NEM inhibition are also subject to modification and thus inhibition by physiological processes. A number of modifications may occur at cysteine residues in proteins, some of them being regulated in a redox-sensitive manner (36). These include nitrosylation (-SNO), hydroxylation (-SOH), disulfide formation (-S-S), conjugation of glutathione (-S-glutathione), and S-palmitoylation. The latter is unique among protein acetylation reactions in that it is reversible. S-Palmitoylation of membrane proteins has been found to influence protein stability, conformation of TMDs, association with specific membrane domains, and interactions with other proteins and other post-translational modifications (37). If palmitoylation at either Cys-33 or Cys-273 were necessary for proper CAT function, the NEM effect could be explained by interfering with this modification. However, as NEM inhibition occurred very rapidly, one would have to postulate a very fast cycle of S-palmitoylation and depalmitoylation of the transporter. The reduced stability of hCAT-2A-CL could, however, be explained by a lack of S-palmitoylation at any of the Cys residues not investigated individually.
A recent report demonstrated inhibition of arginine transport in cardiac myocytes upon stimulation of NO synthesis (38). The authors concluded that "the effect of NO is likely to involve S-nitrosylation of cysteine thiol side chains in these cationic amino acid transporters" and suggested that "putative Cys residues that react with NEM might be also the targets for NOmediated S-nitrosylation." As for S-palmitoylation, there is no consensus sequence deducible from the primary sequences of proteins with cysteine residues known to undergo S-nitrosylation (39). Instead, an acid-base motif in the tertiary structure has been proposed to be engaged in protein-protein interaction facilitating trans-nitrosylation. S-Nitrosylation may also compete with S-palmitoylation and thus has often opposing effects to the latter (40). Future experiments are necessary to show if CAT proteins may be S-nitrosylated or S-palmitoylated and if this occurs at the same Cys residues as alkylation by NEM.