Structure of the Cyanobacterial Phytochrome 2 Photosensor Implies a Tryptophan Switch for Phytochrome Signaling*

Background: Phytochromes are red/far-red photoreceptors using a bilin chromophore. Results: Compared with Cph1, the Cph2 bilin-binding site differs around the propionates, but utilizes an otherwise conserved tongue for sealing the chromophore from solvent. Conclusion: The tongue signals via a tryptophan switch within the tongue-GAF domain interface. Significance: The first structure of a Cph2-type phytochrome indicates a common mechanism for photoswitching in all canonical phytochromes. Phytochromes are highly versatile photoreceptors, which occur ubiquitously in plants as well as in many light-responsive microorganisms. Here, photosynthetic cyanobacteria utilize up to three different phytochrome architectures, where only the plant-like and the single-domain cyanobacteriochromes are structurally characterized so far. Cph2 represents a third group in Synechocystis species and affects their capability of phototaxis by controlling c-di-GMP synthesis and degradation. The 2.6-Å crystal structure of its red/far-red responsive photosensory module in the Pr state reveals a tandem-GAF bidomain that lacks the figure-of-eight knot of the plant/cph1 subfamily. Its covalently attached phycocyanobilin chromophore adopts a highly tilted ZZZssa conformation with a novel set of interactions between its propionates and the GAF1 domain. The tongue-like protrusion from the GAF2 domain interacts with the GAF1-bound chromophore via its conserved PRXSF, WXE, and W(G/A)G motifs. Mutagenesis showed that the integrity of the tongue is indispensable for Pr → Pfr photoconversion and involves a swap of the motifs' tryptophans within the tongue-GAF1 interface. This “Trp switch” is supposed to be a crucial element for the photochromicity of all multidomain phytochromes.

Phytochromes are highly versatile photoreceptors, which occur ubiquitously in plants as well as in many light-responsive microorganisms. Here, photosynthetic cyanobacteria utilize up to three different phytochrome architectures, where only the plant-like and the single-domain cyanobacteriochromes are structurally characterized so far. Cph2 represents a third group in Synechocystis species and affects their capability of phototaxis by controlling c-di-GMP synthesis and degradation. The 2.6-Å crystal structure of its red/far-red responsive photosensory module in the P r state reveals a tandem-GAF bidomain that lacks the figure-of-eight knot of the plant/cph1 subfamily. Its covalently attached phycocyanobilin chromophore adopts a highly tilted ZZZssa conformation with a novel set of interactions between its propionates and the GAF1 domain. The tongue-like protrusion from the GAF2 domain interacts with the GAF1-bound chromophore via its conserved PRXSF, WXE, and W(G/A)G motifs. Mutagenesis showed that the integrity of the tongue is indispensable for P r 3 P fr photoconversion and involves a swap of the motifs' tryptophans within the tongue-GAF1 interface. This "Trp switch" is supposed to be a crucial element for the photochromicity of all multidomain phytochromes.
Phytochromes are red/far-red light absorbing photoreceptors first discovered in plants (1), and later in many photosynthetic and non-photosynthetic bacteria (2) as well as in several fungi (3). A hallmark of these photochromic light sensors is their bilin chromophore covalently bound to a cGMP phospho-diesterase/adenylyl cyclase/FhlA (GAF) 2 domain. Upon red light illumination of its P r state, this chromophore undergoes a Z 3 E isomerization around the C14 -C15 double bond of the C-D ring methine bridge. In the ZZEssa conformation of the newly formed far-red-sensitive P fr state the D-ring is flipped and the protein environment is altered to accommodate this rotation. These alterations ultimately control signal transfer either to effector proteins (inter-molecular (4)) or effector domains like histidine kinase domains (intra-molecular (5)). How these allosteric effects are triggered by D-ring rotation at the molecular level is still unknown thus prompting alternative scenarios for downstream signaling by phytochromes including an additional A-ring rotation upon P r 3 P fr photoconversion (6).
Phytochromes can be classified into subfamilies according to the domain architecture of their photosensory module. Group I includes canonical plant and eubacterial phytochromes such as Cph1 from the cyanobacterium Synechocystis sp. PCC 6803 (SynCph1 (7)) as well as fungal and eubacterial bacteriophytochromes. These phytochromes use a three-domain PAS-GAF-PHY (PAS, period/ARNT/single-minded; PHY, phytochrome) photosensory module, where an unusual figure-of-eight knot is formed by the N terminus of the PAS domain and a loop protruding from the chromophore-binding GAF domain (Fig. 1A). The succeeding PHY domain belongs also to the GAF superfamily and stabilizes the chromophore, by sealing it from solvent access, through its tongue-like extension (8,9). Together, the GAF domain, N terminus, and tongue region form a complex, tripartite pocket enclosing the bilin chromophore. The Cph2 subfamily (Group II) lacks the PAS domain and utilizes instead only a tandem-GAF module as exemplified by the N-terminal photosensory module in Cph2 from Synechocystis sp. (SynCph2) (10) and several other cyanobacterial phytochromes (11). Finally, the cyanobacteriochromes (CBCRs, Group III) utilize a single, bilin-binding GAF domain (11,12). CBCRs cover a broad range of the visible/near UV spectrum but are restricted to cyanobacteria. Whereas, the photosensory modules of several prokaryotic phytochromes from Groups I (8,9) and III (13,14) have been characterized structurally, little is known about Cph2 homologues in Group II.
Interestingly, the founding member of the Cph2 subfamily, SynCph2, is a bimodular photoreceptor (15, 16) (molecular mass 144.7 kDa) that functions as a light-dependent master regulator for cyanobacterial motility by controlling cytosolic levels of the eubacterial second messenger c-di-GMP (17). c-di-GMP is known to be a universal regulator for pili-based motility in eubacteria (18) with domains of the GGDEF and EAL type being responsible for its synthesis and degradation (19). SynCph2 inhibits phototaxis, when cyanobacterial cells are exposed to blue but not to white or red light (20,21). Accordingly, the complex architecture of SynCph2 (Fig. 1A) harbors not only at its N terminus the red/far-red sensitive tandem-GAF module (SynCph2(1-2) (10)) that precedes a GGDEF*-EAL effector module, but also CBCR and catalytically active GGDEF domains. This C-terminal CBCR-GGDEF module is capable of switching between blue and green light sensitive states and controls the catalytic activity of the GGDEF domain, thereby inhibiting motility by increasing c-di-GMP levels (17). The GGDEF*-EAL module likely acts as c-di-GMP degrading phosphodiesterase, as its EAL domain is catalytically active, whereas the apparently inactive GGDEF* domain is predicted to act as an allosteric, c-di-GMP-dependent regulator.
Here, we report the crystal structure of the photosensory module SynCph2 (1)(2) in its P r conformation. An antiparallel dimer is formed in which both the N-terminal PAS domain and the associated knot structures are missing. Other significant differences relative to known phytochrome structures are apparent in the phycocyanobilin (PCB) binding pocket. On the other hand, the interactions between the tongue region of GAF2 and the bilin-binding site of GAF1 are conserved, implying a universal route for signal transduction in Group I and II phytochromes that involves a toggling of conserved Trp motifs within the tongue/GAF1 interface.

Crystallization and Data Collection-Recombinant
SynCph2 (1)(2) was produced as a histidine-tagged fusion with phycocyanobilin co-assembled in vivo. The expression and purification of SynCph2(1-2) was performed as previously described (10). Crystals for the native datasets were grown using the hanging-drop vapor-diffusion method. The drops contained 1 l of 20 mg/ml of SynCph2(1-2)/P r -state in crystallization buffer (10 mM HEPES, 100 mM NaCl, pH 8.0) and 1 l of reservoir solution (0.1 M HEPES, pH 7.0, 1.0 M NH 4 COOH, 0.1% (w/v) lysine, 0.16% (w/v) arginine, 0.05% (w/v) glutamate). Crystals were streak-seeded with a solution of crushed SynCph2(1-2) crystals. After pipetting the pre-irradiated (farred light, max ϭ 735 nm) protein solution under blue-safe light ( max ϭ 482 nm) conditions the plate was irradiated with farred light to ensure 100% occupancy of the P r state. The crystals grew at 18°C in the dark, were visualized under blue light, and frozen in reservoir buffer supplemented with 20% (v/v) glycerol.
Initial SAD phasing was performed with PHENIX (22) using the native 3.20-Å dataset. Subsequent structural refinement with a 2.60-Å dataset was executed with PHENIX and COOT (23). Stereochemical restraints were configured for the PCB chromophore and its covalent attachment. Figures were created by using PYMOL 0.99 (24).
Single-crystal Resonance Raman Spectroscopy-Resonance Raman (RR) spectra were recorded offline at the Cryobench (European Synchrotron Radiation Facility (ESRF), Grenoble (France)) with a 785 nm near-IR laser using an inVia (Renishaw) Raman spectrometer (4 cm Ϫ1 spectral resolution) (25). Resonance Raman spectra of the crystal (1 M MgOAc 2 as cryo-protectant) were collected under cryo-conditions (100 K) in the 200 -2000 cm Ϫ1 range using the SynchroScan method with a laser power of 300 milliwatts (50 milliwatts at the sample position) and 20-s exposure time. 30 -50 spectra were accumulated and baseline corrected to remove contributions from fluorescence and normalized to the 1642 cm Ϫ1 peak. Resonance Raman spectra were recorded before x-ray exposure, and then the crystal was irradiated like during data collection (ID 14 -1, 100% transmission, 4 s exposure time per image, 350 images, 0.5°oscillation range; flux: ϳ4.8 ϫ 10 10 photons/s; the crystal size was ϳ30% of beam size so that a dose of ϳ1.4 ϫ 10 10 photons/s reached the crystal). The crystal was characterized spectroscopically and then annealed by short thawing for 4 s in the dark, whereupon another Raman spectrum was taken. The crystal remained under cryo-conditions during the whole data collection/spectra recording process.
Single-crystal UV-visible Absorption Spectroscopy-UV-visible spectra of the crystal were measured online at 100 K with a HR2000 spectrometer (OceanOptics) and a DH-2000-BAL light source (Mikropack) at beamline ID 14-1 (ESRF, Grenoble (26)). Spectra were taken before x-ray irradiation, immediately after irradiation (60 s exposure; 100% transmission; flux: ϳ4.2 ϫ 10 10 photons/s; crystal size ϳ30% of x-ray beam diameter), after a 3-s cryo-annealing and subsequent freezing, and after a 60-s incubation under cryo-conditions following x-ray irradiation. Initial two exposure pictures were taken following a 3-s exposure per image with a 1.0°oscillation.
Quantum Mechanical/Molecular Mechanical (QM/MM) Calculations-The initial structural models were set up by adding missing hydrogen atoms and solvent water to the experimental x-ray structure. Two models were considered in this work: (a) the crystal structure of SynCph2(1-2) itself and (b) a modified structure of SynCph2(1-2) with a planar PCB chromophore as found in the structure of the SynCph1 photosensory module (8).
The insertion of hydrogen atoms was done with the HBUILD routine of CHARMM (31). All titratable amino acids were protonated according to pH 7. In the case of the histidines, the protonation states were chosen upon visual inspection of their environment. In particular, His 130 and His 160 , lying in the vicinity of the chromophore, were modeled with a hydrogen atom at the ⑀-nitrogen and ␦-nitrogen, respectively. In addition, based on spectroscopic evidence (27,28) the PCB chromophore is nitrogen-protonated, whereas the propionic side chains are deprotonated yielding a total charge of Ϫ1e.
The geometry optimization of the PCB chromophore and binding site of Cph2 and Cph1 phytochromes, comprising all atoms within a 22-Å radius from the chromophore, were performed at the QM/MM level (29). Although the QM fragment, consisting of the PCB chromophore, the Cys 129 side chain, and the pyrrole water that is bound to rings A, B, and C nitrogens, were described with the B3LYP density functional (30), the remaining protein atoms as well as solvation waters with the CHARMM22 force field (31). The coupling between the QM and MM regions was described with the electrostatic embedding model combined with a charge shifted scheme (32), as implemented in the ChemShell software. The covalent bond cut at the QM/MM border on the Cys 129 was saturated with a hydrogen link atom. The optimization was performed using a limited memory quasi-Newton L-BFGS algorithm working with hybrid delocalized internal coordinates (33).
UV-visible absorbance spectra were measured with a Jasco V-660 spectrometer at 1-nm resolution at room temperature. In a cell of 1-cm path length the sample was irradiated for 30 s with red or far-red LED sources (B5-436-30D, max 664 nm and SMC735, max 735 nm; Roithner, Vienna). Difference spectra were calculated via A(P r ) Ϫ A(P Photoequilibrium ). Determined pure P fr spectra of the wild type are discussed in Ref. 10.
Near UV-visible circular dichroism (CD) spectra were measured following irradiation of the sample with a J-810 spectropolarimeter (Jasco) at room temperature in a 1-mm path length cell. P r spectra were averaged over three scans. Short-lived intermediate or P fr states were averaged over six scans where the sample was irradiated in between.

RESULTS AND DISCUSSION
Overall Structure of the SynCph2 Photosensor-The N-terminal photosensory module of SynCph2 (M1-T424, (10)) in its P r state crystallizes as an antiparallel dimer whose structure was solved at 2.6-Å resolution by a combination of molecular replacement and single-wavelength anomalous diffraction (SAD)-phasing (final R factor /R free , 19.6/25.3%, see Table 1).
The GAF1 domain with its buried PCB chromophore resembles the bilin-binding domains of canonical phytochromes such as SynCph1 and RpBphP3 (root mean square deviation (r.m.s.) deviations of 0.98 and 1.02 Å for 175 and 164 C ␣ atoms, respectively). In contrast, the core of the GAF2 domain diverges significantly from known PHY domains yet remains more closely related to PHY domains from biliverdin-dependent bathy phytochromes such as RpBphP3 or PaBPhP (2.26 and 2.82 Å for 117 and 135 C ␣ atoms, respectively) than to PCB-dependent phytochromes like Cph1 (3.40 Å for 150 C ␣ atoms). Structural

TABLE I Data collection and refinement statistics
Values in parentheses denote the highest resolution shell.
differences are mostly around the five-pleaded central ␤-sheet, including the elongated loops connecting ␤-strands 8 -9 and 11-12 as well as the ␣-helices covering the ␤-sheet opposite the ␣-helix linking GAF1 and GAF2 (Fig. 1B). Furthermore, in both dimer subunits an additional stretch of 27 amino acids (Ala 282 -Thr 308 ) is structurally disordered as indicated by missing electron density. Interestingly, this stretch is unique for Cph2 as it is absent from PHY domains of Group I phytochromes.
Crystals of SynCph2 are cyan-colored due to the incorporated PCB cofactor and give the same UV-visible and resonance Raman spectra as in solution. Accordingly, this proves that SynCph2(1-2) adopts a native-like conformation for its bilin chromophore in the crystalline state ( Fig. 2) (35).
Despite its monomeric state in solution (10) SynCph2(1-2) forms an antiparallel dimer in crystals similar to the photosensory module of SynCph1 (Fig. 3). Its interface area is predicted by the PISA server to be ϳ1904 Å 2 and thus considerably smaller than that of SynCph1 (2545 Å 2 ) (8) due to the missing PAS domain. Its staggered and antiparallel orientation differs from other known phytochrome structures such as PaBPhP, in which a parallel association is observed over the whole length of the GAF-PHY linker helices. The latter mode of association is thought to be crucial for signal transduction to histidine kinase domains (9,36) and illustrated by the cryoEM structure of fulllength bacteriophytochrome DrBphP, where the photosensory module provides the largest dimerization interface. Like in parallel dimers of the photosensory phytochrome modules, the antiparallel arrangement of SynCph2(1-2) is mainly stabilized by association of its linker ␣ 5 -helices between GAF1 and GAF2 (Figs. 1B and 3A). Interestingly, the ␣ 5 -helices resemble those of the Y263F SynCph1 mutant (37), whereas in the wild type SynCph1 (Fig. 3B) and PaBphP structures these helices are significantly kinked. Some structural plasticity between the GAF and PHY domains as observed for PaBphP and SynCph1 (8,9,37) may be involved in long-distance signal transmission. Accordingly, the GAF2 domains of the two copies of SynCph2 (1)(2) in the crystal structure are off-rotated by 6.4°relative to each other, whereas the tongue regions are almost invariantly associated with the GAF1 domains.
Another striking feature of SynCph2(1-2) is its missing figure-of-eight knot, a hallmark of Group I phytochromes. In SynCph1 the N-terminal helix threads through the loop region connecting ␤ 9 with ␣ 8 and is part of the PCB binding pocket. The Group II SynCph2(1-2) phytochrome lacks this knot along with the associated PAS domain, so that the binding pocket is partly solvent-exposed at the A-and B-rings of the PCB chromophore (Fig. 3B). Instead, the N-terminal ␣ 1 -helix of SynCph2(1-2) is part of a six-helix bundle comprising ␣ 1 , ␣ 5 , ␣ 6 , and ␣ 10 of one monomer and ␣ 2 and ␣ 5 of the other monomer (Fig. 3A). In this interface, the amphiphilic ␣ 1 -helix makes first several polar interactions via its residues Arg 5 , Lys 17 , and His 19 with Glu 34 and Glu 181 from ␣ 2 and ␣ 5 , respectively. Second, for hydrophobic interactions the ␣ 1 -helix packs its residues Leu 7 , Phe 10 , Leu 11 , Val 14 , and Phe 18 against a surface patch generated by Val 30 , Ile 31 , Phe 38 , Leu 177 , and Leu 178 of ␣ 2 and ␣ 5 . Accordingly, one may hence postulate that the dimerization of PAS-less phytochromes like SynCph2(1-2) depends on their N-terminal helix.
The different output domains, especially the GGDEF*-EAL module downstream of the SynCph2(1-2) sensor, imply that the antiparallel association of the photosensory domains could be functionally relevant in SynCph2 and not just a crystallization artifact as found for SynCph1 (8). For example, FimX from Pseudomonas aeruginosa, a twitching motility regulator, also carries GGDEF-EAL domains. Here, the overall quaternary organization corresponds to an elongated antiparallel dimer, where the GGDEF-EAL modules are not involved in dimerization (38). Such an arrangement of the photosensory SynCph2(1-2) module may be of physiological relevance for signaling toward the GGDEF*-EAL module. This contrasts to eubacterial phytochromes such as SynCph1, which are part of two-component signaling systems. Only these phytochromes have to form parallel homodimers (36) similar to the structurally characterized sensor histidine kinases YF1 and VicK (39,40) to allow proper dimerization and thereby catalytic activity of their histidine kinase domain-like regions.
The PCB-binding Site of SynCph2(1-2)-Overall, the bilin chromophore is well defined in the GAF1 domain including its thioether linkage to Cys 129 (Fig. 2C). Despite structural differences in the protein environment relative to other Group I phytochromes, mainly around pyrrole rings A and B, the conformation of the chromophore resembles SynCph1. First, the new chiral center at C3 1 , as formed by covalent attachment to Cys 129 , has an R configuration. This may not be a general feature for bilin chromophores covalently tethered to GAF domains; for example, in the structure of the Y263F mutant of SynCph1 both stereochemical configurations have been found for the C3 1 -Cys thioether linkage (37). Second, and as expected, for the SynCph2(1-2) P r state, the chromophore adopts the ZZZssa conformation (Fig. 2C, supplemental Table  S1), but in a strongly non-planar conformation, when compared with other phytochrome structures harboring bilin chromophores.
Despite the latter, the local environment of the D-ring is well conserved between SynCph2 and SynCph1 as exemplified by the residues Tyr 47 , Val 57 , Asp 79 , Tyr 133 , Leu 134 , Met 137 , and His 160 . Differences here are mainly found for Lys 45 or Leu 70 , but appear to only play a minor role in the P r 3 P fr conversion, as the SynCph2 K45M mutant shows only a ϳ3 nm hypsochromic shift in its P fr state. Other residues of the C-and D-ring binding  (1-2) in soluble and crystallized forms. A, UV-visible absorbance spectra of SynCph2 (1)(2) in solution at room temperature (red curve) and a crystal (black) at 100 K in the P r conformation. B, Raman spectra of the protein in frozen solution (red curve) at 100 K and in crystalline form (black) at 100 K. C, omit electron density of the PCB chromophore and its covalent attachment to Cys 129 (contouring level 1.0 ). site like Tyr 133 are functionally similar to their structural counterparts in SynCph1 (Tyr 263 ) as indicated by the similar photochemistry of their mutants, for example, Y133F or H160A (supplemental Fig. S1 and Table S2) (37).
The loop connecting ␤ 5 and ␣ 4 in SynCph2, which is otherwise part of the knot region of Group I phytochromes, is partly disordered due to a lack of further interactions. Furthermore, the SynCph2 loop appears to interact with the B-ring propio-nate differently than in SynCph1. SynCph2 forms an additional ␤-strand (␤ 5 , Arg 102 -Glu 108 ), of which Lys 104 makes a surfaceexposed salt bridge with the B-ring propionate (Fig. 4A). In SynCph1 as well as other Group I phytochromes this propionate interacts with Arg 254 (loop region) in P r , but probably swaps partners upon photoconversion to interact with Arg 222 , part of ␤-strand 9 (41) (Fig. 4B). Both of these arginines are missing in SynCph2. Here, Lys 104 apparently substitutes for Arg 254 in SynCph1. However, an equivalent interaction partner for the B-ring propionate in the P fr state seems to be missing. Thus the propionate signaling route envisaged for Group I phytochromes (8) is unlikely for SynCph2. Multiple sequence alignments of Group II phytochromes (Fig. 5) show that Lys 104 is conserved in other cyanobacterial phytochromes with the same complex domain architecture of SynCph2. In Group II phytochromes with different architectures, for example, GAF-GAF-GGDEF*-EAL (17) or GAF-GAF-histidine kinase domain, this residue is replaced by polar or isoleucine residues. Interestingly, Cph2 orthologues with downstream histidine kinase effector domains harbor both SynCph1 arginines (Arg 222 and Arg 254 ). It seems, therefore, that the propionate interactions may vary according to the route required for signal transmission to the effector domains.   (1)(2) were performed with the BLOSUM62 exchange matrix. Letters at the end of the abbreviation describe the domain architecture of the proteins described below: C indicates conserved cysteines; green, GAF domain of the "PHY"-type; blue, GAF domain of the "CBCR"-type; white, GAF domain with sequence similarities with "CBCR"-type but with one or no conserved cysteine. Mixed colors, gene-dependent domain organization as indicated by the organism abbreviation. DECEMBER 13, 2013 • VOLUME 288 • NUMBER 50

JOURNAL OF BIOLOGICAL CHEMISTRY 35719
The unusual interactions of the B-and C-ring propionates are not crucial for P r 3 P fr photoconversion, however. Alanine mutants of Lys 104 and the neighboring amino acids Arg 103 and Lys 105 show wild type P r 3 P fr photoconversion. Only a ϳ3 nm hypsochromic shift is observed for the P r and P fr states of K104A. Even swapping variants of SynCph2 (1)(2), where the whole region around the B-ring propionate (Val 97 -Val 126 ) is exchanged with regions of other phytochromes of the Cph2 subfamily, show almost normal P fr (supplemental Fig. 1 and Table S2).
A study of the B-and C-ring propionate amide adducts in SynCph1 reveals that a free B-ring propionate is essential for wild type P r and that full photoconversion to P fr requires a conformational switch at the C-ring propionate (42). Apart from the missing arginines in SynCph2 B-ring propionate interactions, the C-ring propionate interactions are similar in SynCph1 and SynCph2 and involve residues Thr 144 and His 130 , as well as a water molecule.
SynCph2 (1)(2) Induces the Tilted PCB Conformation-An unusual feature of the PCB chromophore compared with other phytochrome and even phycobilisome-PCB structures is the strong tilt between its constituent rings (see supplemental  Table S1). The chromophore shows tilts of 19.2°, 32.5°, and 59.8°between rings A-B, B-C, and C-D, respectively (in comparison SynCph1: 9.8°, 1.5°, and 26.3°). The tilt between rings B-C and C-D is hence the largest among known phytochromes and PCB-binding phycobilisomes. Only non-conjugated chro-mophores in phycobiliproteins showed so far similar or higher tilts (see supplemental Table S1). Given earlier suggestions that the phytochrome chromophore conformation might generally be damaged by x-ray radiation (6), we validated the observed PCB conformation by a combination of single crystal UV-visible and resonance Raman spectroscopy with QM/MM geometry calculations based on structural models of SynCph2 (1)(2). Upon x-ray irradiation of crystals the spectral signatures of the P r state of SynCph2 (1)(2) are changed. First, in the UVvisible spectrum an additional shoulder at 555 nm appears, whereas the signal at 644 nm decreases and the maximum shifts to 646 nm (Fig. 6A) accompanied by a broadening of the 646 nm peak. Second, in single-crystal Raman spectra (Fig. 6B) x-ray exposure of SynCph2(1-2) crystals cause additional Raman signals at 641 cm Ϫ1 and 1223 cm Ϫ1 , a signal increase at 811 cm Ϫ1 and 1454 cm Ϫ1 , and a slight peak broadening between 1550 and 1700 cm Ϫ1 . Only cryo-annealing restores the pre-x-ray UVvisible and resonance Raman spectra. Under cryogenic conditions, after x-ray exposure, a slow relaxation process takes place within minutes (Fig. 6C), where the shoulder at 555 nm in the UV-visible spectrum decreases but the signal at ϳ644 nm remains unchanged. Again cryo-annealing allows complete reversal to the P r state before x-ray irradiation.
These data are in line with previous observations on the photosensory module of SynCph1, where long x-ray radiation induced additional absorbance peaks in the UV-visible spectra (43). In SynCph2(1-2) the x-ray-induced species relaxes in at  (1-2). Black, no x-ray; red, after x-ray; green, after annealing; blue, 60 s after exposure. A, UV-visible absorbance spectra of a crystal before x-ray, after 60 s exposure (ID 14-1 (ESRF, Grenoble), 100% transmission; flux: ϳ4.2 ϫ 10 10 photons/s (approximately ϳ1.4 ϫ 10 10 photons/s reached the crystal)), and after 3 s of annealing in the dark (annealing process was not complete). B, Raman spectra of a crystal before x-ray, after 30 min x-ray irradiation (ID 14 -1 (ESRF, Grenoble), 100% transmission, 4-s exposure time per image, 350 images, 0.5°oscillation range; flux: ϳ4.8 ϫ 10 10 photons/s) and after 4 s of annealing in the dark; below: partial scale-up of the Raman spectra. C, UV-visible absorbance spectra of a crystal before x-ray, after exposition for 60 s, and 60 s after exposure. least two steps. At 100 K it decays to a further species without recovery of the P r maximum (Fig. 6C). Apparently, the activation energy needed for conversion into native-like P r cannot be overcome and the chromophore of SynCph2(1-2) remains hence arrested in an intermediate state. Further relaxation to P r , which means recovery of the spectral characteristics of SynCph2(1-2) crystals, proceeds only at higher temperature.
The resonance Raman spectra indicate that alterations occur near the ring B and C nitrogens and could be caused by the pyrrole water that is hydrogen bonded to the pyrrole nitrogens. However, because the absolute positions of the Raman bands, in particular those in the fingerprint region between 1500 and 1700 cm Ϫ1 (Fig. 6B), are virtually identical in irradiated-and non-irradiated samples, one may infer that the main conformation of the tetrapyrrole chromophore is undistorted upon irradiation. Thus, x-ray damage is not per se responsible for the very large tilts observed between rings B and C.
To assess the significance of the tilt between rings B and C, for example, excluding that the tilts are caused by the nature of the parameters used during crystallographic refinement, we optimized the structure of the PCB binding site of SynCph2(1-2) using a QM/MM approach as described under "Experimental Procedures." The geometry of PCB converges to a conformation, which is only slightly different than in the crystal structure, as indicated by a r.m.s. deviation of only 0.4 Å. In particular, the large twist observed at the B-C methine bridge (Table 2) is maintained (23°) and exceeds those observed for any other phytochrome. In the optimized structure, the protein environment around the chromophore remains largely conserved. Analogous results are obtained for a SynCph2 planar model, where the chromophore of SynCph2 is substituted by the nearly planar PCB chromophore from the SynCph1 structure. QM/MM calculations based on this model again provide a non-planar PCB chromophore that is strongly twisted at the B-C methine bridge (18°). These calculations indicate that the large tilt between the B-and C-rings is indeed enforced by the SynCph2 protein matrix rather than by radiation damage.
Interactions of the Tongue Region with the PCB-binding Pocket-The hairpin-like tip of the GAF2 tongue region, which contacts the GAF1 domain, is highly conserved among Group I and II phytochromes. However, the stem regions of the tongue connect differently to the GAF and/or PHY domains in different homologs (Fig. 7A). These differences are exemplified by FIGURE 7. The tongue-like region of SynCph2(1-2). A, structure and interactions between the tongues and the chromophore-bearing GAF domains of phytochromes. B, comparison of the tongue regions of SynCph2 and SynCph1, superimposition of the GAF domains (r.m.s. deviation ϭ 1.095 Å for 125 C ␣ ); GAF1 and GAF2 of SynCph2 are displayed in orange and green; PAS, GAF, and PHY of SynCph1 in blue, gray, and pale red, respectively. The chromophores are shown in cyan (Cph2) and red (Cph1). The positions of the PRXSF, W(G/A)G, and WXE motifs in the tongue regions are conserved between SynCph1 and SynCph2, the residues are numbered along the SynCph2 count. C, tongue region of PaBphP (PDB code 3NHQ), the PAS (blue), GAF (gray) and PHY (red) domains and biliverdin in green. In contrast to SynCph2 and SynCph1 the tongue region consists of an extended loop region and ␣-helical elements. The spatial positions of the conserved motifs differ in PaBphP. The orientation of the tongue region correlates to that of SynCph2.

TABLE 2 Ring tilts of PCB from x-ray crystallography and QM/MM calculations
Cph2 refers to the crystal structure of SynCph2(1-2). The planar geometries relate to a modified SynCph2(1-2) structure, where the chromophore was substituted by the PCB chromophore from the SynCph1 structure (PDB 2VEA).  DECEMBER 13, 2013 • VOLUME 288 • NUMBER 50 the structure of PaBphP in the P fr state (Fig. 7C), where a mostly ␣-helical conformation is adopted.

Tilt A-B Tilt B-C Tilt C-D
Although in SynCph2 the ␤-strands (␤ 13 , ␤ 14 ) forming the stem of the tongue are longer than in SynCph1 (the GAF2 domain is tilted further away from GAF1 than the PHY domain in SynCph1), the overall positions of the conserved W(G/A)G, PRXSF, and WXE motifs relative to the GAF1 domain are well conserved between the two (Fig. 7B). Only in the P fr state of PaBphP do these motifs differ significantly (Fig. 7C), allowing only the ␣-helical part of the tongue to cover the PCB binding pocket and displacing the W(G/A)G and WXE motifs by 11-15 Å relative to their counterparts in SynCph2 and SynCph1.
In SynCph2 the W(G/A)G motif (Trp 369 -Gly 371 ) extends from ␤ 13 into the hairpin tip region of the tongue. This region is distal from the GAF1 domain by being located on an elongated loop region in PaBphP/P fr (Fig. 7C). In the SynCph2/P r structure, Trp 369 of the W(G/A)G motif forms an H-bond via the indole nitrogen with Glu 391 from the WXE motif on ␤ 14 . The role of the former anchor-like WGG motif is underlined by the finding that the W369A mutant remains arrested in an intermediate state upon red light illumination. The same is observed for the W389A mutation within the second anchor of the tongue, the WXE motif, but not for the E391A mutant, a fact suggesting that the interaction of the latter with Trp 369 is not crucial for photoconversion. Given the large distance of 9 -14 Å to the PCB binding pocket in the P r state of SynCph1 and SynCph2, the involvement of the Trp motifs in P fr formation conversion is elusive. As mutant Group I phytochromes in which the PHY domain has been deleted, are known to be unable to form bona fide P fr , one may conclude that the bulky side chains of these tryptophans are required to stabilize not only the P r state, but also an alternative conformation of the tongue when switched to the P fr state. Indeed, mutations of Trp 369 or Trp 389 to phenylalanine in SynCph2 allow P fr formation ( Fig. 9), albeit with compromised efficiency for the W369F mutant (supplemental Fig. S2). This underlines the importance of the general bulky aromatic character of these tryptophans for the structural reorganization of the tongue during P r 3 P fr photoconversion. When comparing the SynCph2 and SynCph1 P r structures with the PaBphP/P fr structure, it can be observed that in both states a tryptophan is sandwiched at almost the same position in the tongue-GAF1 interface. However, in P r the indole moiety comes from the W(G/A)G, but in P fr from the WXE motif. In this way, the swap of the bulky tryptophan side chains in the tongue-GAF1 interface may support the bistability and hence photochromicity of Group I and II phytochromes.
The third conserved motif within the tip of the tongue, the PRXSF motif, shields the entrance to the PCB binding pocket by adopting an elongated conformation above it and allowing for Arg 383 to project into the GAF1 domain where it forms a salt bridge with Asp 79 of the DIP motif. The conformation of the PRXSF motif, including the outward orientation of the serine residue and the backbone conformation until the WXE motif, is absolutely conserved between SynCph2 and SynCph1 (Figs. 7B and 8A). Mutants of the salt bridge involving the PRXSF motif like R383D or D79R remain hence either arrested in an intermediate state (10), or are photochemically impotent (supplemental Fig. S1).
Pro 382 from the PRXSF motif is directly placed above the thioether bridge between Cys 129 and ring A of the PCB chromophore. Together with Pro 81 of the DIP motif, Pro 382 forms a clamp hindering large conformational changes of the A-ring. Interestingly, earlier NMR studies on the P fr -like state of an isolated GAF domain of another Group II phytochrome postulated A-ring rotation as part of the photocycle (6). Given that this ubiquitous structural restraint in Group I and II phyto-  Fig. 7. B, the tongue region seals the PCB-binding pocket. In molecule B the whole tongue is defined by electron density. Only Arg 383 of the PRXSF motif (spheres) is depicted as stick representation. Hydrogen bond distances are given in Å. C, chain A shows that the tip of the tongue is rather motile as several residues are missing and Arg 373 adopts a different conformation.
chromes is missing in that case, it is feasible that the tongue-GAF interaction serves to exclude alternative isomerization pathways for bound bilin chromophores. Clearly, a disturbance of the A-ring clamp as given by the SynCph2 P382G mutant causes a lack of photoconversion to P fr (supplemental Fig. S2) and again suggests that a rigid element is needed here to allow conformational P r 3 P fr switching. Interestingly, a threonine mutant of Pro 382 photoconverts to P fr like the wild type but exhibits a faster dark reversion (supplemental Fig. S2). Accordingly, this backbone version requires only partial restriction of its conformational space to maintain the function of the tongue motif.
Finally, the S385A and F386A mutants, like most of the other mutants of the tongue motifs, fail to form P fr upon red light illumination. Phe 386 is buried in the tongue-GAF1 interface close to the hydrophobic environment of ring D and thus stabilizes at least the P r state. In contrast, the surface-exposed Ser 386 makes no interactions to any other residue in the SynCph2 structure. When comparing the P r state structures of SynCph2 and SynCph1 with the P fr state of PaBphP, it is apparent that the PRXSF motif adopts an inverted orientation in the latter; the serine instead of the arginine points into the binding pocket to build a hydrogen bond to the aspartate of the DIP motif. The role of this serine is further underlined by the fact that a mutation to alanine destabilizes the P fr state of PaBphP (9). Together with the role of the tryptophan motifs one can suggest that during conversion into P fr the salt bridge is released and the tip of the tongue undergoes huge conformational changes (Fig. 10). According to this model these residues are crucial for conformational stabilization of the P fr state and thus for complete photoconversion. Interestingly, in the case of the SynCph2(1-2) module, such a large conformational change is indicated by an increased ␣-helical content and hydrodynamic diameter upon P fr formation (10).
Apart from the conserved motifs mentioned above for the tongue tip there is some length variation of different phytochromes, which is illustrated by the 5-amino acid larger tongue tip for SynCph1 than for SynCph2. The tip of the loop in SynCph2 adopts alternative conformational states within the crystal. In chain A of SynCph2 the Asp 79 -Arg 383 salt bridge is part of a hydrogen-bonding network consisting of Tyr 133 , together with a second arginine from the tip of the tongue (Arg 373 ) as well as a water molecule. The latter stabilizes the tongue-GAF1 interaction by coordinating the amide nitrogen of Arg 383 and the ring A carbonyl group (Fig. 8C). In chain B Arg 373 flips out and interacts with Arg 378 and Glu 78 . This interaction stabilizes the loop region, which can be clearly defined by electron density (Fig. 8B). However, in both molecules, Arg 383 H-bonds to the backbone carbonyl of Ala 370 of the W(G/A)G motif. The tongue-GAF1 interaction is further stabilized by an interaction absent in SynCph1. Lys 367 , located in ␤ 13 of the tongue contacts the edge of the GAF1 domain via an extended hydrogen bond network between His 74 and Ser 54 .
The Tongue Region as a Functional Module within Phytochromes-Given the strict dependence of the P fr conformation on conserved motifs within the tongue region, we wondered whether the tongue extension presents on its own a functional module that is interchangeable between different phytochromes of the Cph2 family. We generated swapping variants of SynCph2(1-2) in the tongue region by replacing the FIGURE 9. Mutants and hybrids of the SynCph2 tongue region. A, UV-visible absorbance (left) and CD spectra (right) of SynCph2(1-2) wild type and mutants after far-red (P r -state, black line) and red light illumination (red). Difference spectra (A Pr Ϫ A Photoequilibrium ) are shown in blue. Green curves in the wild type spectra are calculated for pure P fr (10). The partial lack of a wild type-like P fr CD spectrum of Oscillatoria tongue is due to its instability. B, multiple sequence alignment of Group II phytochromes for GAF2 sequence stretches defining the tongue region. Tongue regions swapped between hybrid variants are depicted in black, stretch between the W(G/A)G motif and the WXE motif (Trp 369 -Glu 391 ) with regions of orthologous Group II phytochromes from N. punctiforme, Oscillatoria sp. and Cyanothece sp. (see Fig. 9 and supplemental Fig. S2). The Oscillatoria sp. and Cyanothece sp. tongue-containing variants exhibit normal photoconversion, whereas the Cyanothece sp. tongue-containing variant showed only partial P fr formation after red light illumination. Interestingly, the variant with the N. punctiforme tongue displayed incomplete photoconversion. This tongue harbors a degenerated PRXSF motif due to an arginine to glutamine substitution. Nevertheless, a R383Q mutant in SynCph2(1-2) showed normal P r /P fr photoconversion indicating that in the P r state glutamine can form analogous H-bonds with Asp 79 as the arginine and that in the P fr state other residues are at least equally crucial for the proper association of the tongue with the GAF1-bilin binding site.
The Tongue, a Subdomain for P fr Stabilization and/or Signaling?-So far, the light-triggered Z 3 E isomerization around the C15-C16 double bond is insufficient to rationalize the strong bathochromic shift of the P fr state of Group I and II phytochromes by about 100 -130 nm when compared with simple bilin-chromopeptides. Clearly, the complex bilin-binding site of phytochromes stabilizes these P fr photo states by an unresolved mechanism, e.g. by electrostatic interaction, steric control of bilin conformation, or even formation of enol-like tautomers (44). Our structural and mutagenesis data on SynCph2 indicate that the conserved PRXSF, W(G/A)G, and WXE motifs of the tongue region are crucial for P fr formation. Other structural elements, which contribute to the bilin-bind-ing site of a canonical phytochrome such as SynCph1 (8,9), are either missing (N terminus) or replaced like the subsite for ring B and C propionates. The W(G/A)G and WXE motifs are shared by Group I and II phytochromes and affect P r 3 P fr photoconversion due to their distance and link to the bilin binding pocket via a conserved H-bonding network. Together with structural data on the P fr state of bathy phytochromes (9) we suggest conformational switching of the tongue region via swap of the tryptophans in the tongue-GAF1 interface during P r 3 P fr conversion (Fig. 10). This folding event on the local protein level is predicted to be part of the slow, light-independent lumi-R 3 P fr conversion that occurs in the millisecond range. Accordingly, bathy phytochromes may prefer P fr as the paternal state to P r due to different energetics of the interaction between their chromophore/GAF1-adduct and the tongue region. Slow protein conformational changes such as the suggested Trp switch are instrumental for further signal transfer either to the C-terminal effector domains as in bacterial phytochromes or for modulating protein-protein interactions as in plant phytochromes. Interestingly, the tongue region of known phytochrome structures passes into a helix that directly connects the PHY/GAF2 domains with downstream effector modules and thus provides a short route for intra-molecular signaling. This may suggest that different effector domains such as histidine kinases, diguanylate cyclases, and c-di-GMP-specific phosphodiesterases share a common mechanism for intramolecular signal transduction.