NRA-2, a Nicalin Homolog, Regulates Neuronal Death by Controlling Surface Localization of Toxic Caenorhabditis elegans DEG/ENaC Channels*

Background: Mechanisms that regulate plasma membrane expression of neuronally toxic DEG/ENaC channels are unclear. Results: Disruption of ER NRA-2, a Nicalin homolog, enhances C. elegans MEC-10(d) DEG/ENaC neurotoxicity. Immunocytochemistry, TIRF imaging, and electrophysiological assays support that NRA-2 controls relative MEC-10(d) distribution between ER and cell surface to regulate channel activity levels. Conclusion: NRA-2 regulates surface expression of a mutant DEG/ENaC channel. Significance: NRA-2 Nicalin can modulate DEG/ENaC pathophysiology. Hyperactivated DEG/ENaCs induce neuronal death through excessive cation influx and disruption of intracellular calcium homeostasis. Caenorhabditis elegans DEG/ENaC MEC-4 is hyperactivated by the (d) mutation and induces death of touch neurons. The analogous substitution in MEC-10 (MEC-10(d)) co-expressed in the same neurons is only mildly neurotoxic. We exploited the lower toxicity of MEC-10(d) to identify RNAi knockdowns that enhance neuronal death. We report here that knock-out of the C. elegans nicalin homolog NRA-2 enhances MEC-10(d)-induced neuronal death. Cell biological assays in C. elegans neurons show that NRA-2 controls the distribution of MEC-10(d) between the endoplasmic reticulum and the cell surface. Electrophysiological experiments in Xenopus oocytes support this notion and suggest that control of channel distribution by NRA-2 is dependent on the subunit composition. We propose that nicalin/NRA-2 functions in a quality control mechanism to retain mutant channels in the endoplasmic reticulum, influencing the extent of neuronal death. Mammalian nicalin may have a similar role in DEG/ENaC biology, therefore influencing pathological conditions like ischemia.

Under conditions of localized oxygen deprivation in the brain, neuronal energy deficits promote an excitotoxic cascade in which ion channels are excessively activated to induce neuronal necrosis (1). Although the primary insult is often localized to a small site, successive rounds of secondary neuronal death ensue as dying neurons release channel-hyperactivating gluta-mate to lethally excite their neighbors, expanding the region of cell death to markedly exacerbate functional losses. Understanding the molecular mechanisms operative in necrotic neuronal death is critical for the design of neuroprotective strategies, although challenges of in vivo dissection of the death process in higher organisms are substantial.
Genetic dissection of necrosis mechanisms in model organisms has revealed conserved pathways that contribute to necrotic death (1,2). In C. elegans, specific gain-of-function mutations in DEG/ENaC family channel subunits induce the necrotic death of the neurons in which they are expressed (3)(4)(5). MEC-4, a pore-forming subunit of the mechanosensory channel complex in C. elegans touch receptor neurons (TRNs) 3 that is required for sensing gentle mechanical stimuli (typically delivered to the animal as a brush with an eyelash hair), can be altered to a neurotoxic form (4). Large side-chain amino acid substitutions for a small amino acid near the pore domain (MEC-4(A713V), referred to here as MEC-4(d)) create a hyperactivated channel that is permeable to Na ϩ and Ca ϩ2 (6 -8). Calcium dysregulation in mec-4(d) neurons is amplified by calreticulin-dependent release of calcium from ER stores (7,9), which activates cytosolic calpain proteases (10) that ultimately influence deleterious lysosomal activity (11). That the mammalian neuronal DEG/ENaC channel subunit ASIC1 contributes to necrotic death in mouse ischemia models (12)(13)(14) supports that conserved mechanisms of neuronal DEG/ENaC hyperactivation toxicity are operative across phyla.
MEC-10 is a second DEG/ENaC channel subunit co-expressed with MEC-4 in C. elegans TRNs (3), and assembling with additional subunits stomatin MEC-2 and paraoxonase MEC-6 to form a touch-transducing complex (8). Introduction of an engineered mec-10 transgene encoding the mec-4(d)analogous toxic amino acid substitution (namely A693V) to create mec-10(d) induces a low level of necrosis associated with neuronal swelling and featuring a similar toxicity mechanism to mec-4(d) (15). The low level of toxicity associated with the mec-10(d) transgene presents an opportunity to screen for genes that can mutate, or be knocked down with RNAi approaches, to enhance necrosis. Such genes would be anticipated to identify negative regulators of channel biosynthesis and activity or to define factors that normally protect against downstream neurotoxic events.
Here we report that disruption of mammalian nicalin homolog nra-2, an ER protein implicated in C. elegans muscle acetylcholine receptor (AChR) channel maturation (16), enhances MEC-10(d)-induced necrosis via a mechanism that increases surface expression of the MEC-10(d) channel. Our data further underscore the role of excessive channel activity in necrosis induction, identify NRA-2/nicalin as a new player in DEG/ENaC channel biogenesis, and reveal an unexpected focused surface distribution of the MEC-10(d) channel in touch neurons. Because mammalian DEG/ENaC functions modulate cystic fibrosis, renal diseases, hypertension, and ischemia-associated neurotoxicity, insights into regulated surface expression might be exploited in design of interventions that modulate channel activity in human disease.

C. elegans Strains
Nematodes were maintained according to standard procedures (17). Transgenic strains were generated as described (18). RNAi was executed according to, (15)

Cell Death Assay
The percentage of TRN degeneration was scored as described before (15). Briefly, mCherry-labeled TRNs were counted in Is[mec- 10(d)] and nra-2(ok1731); Is[mec-10(d)] strains using a Zeiss Imager.D1m microscope and a Zeiss Pla-nApochromat 20ϫ NA 0.8 objective. The average number of surviving TRNs per animal was calculated as the total number of TRNs counted divided by the total number of animals counted.

C. elegans Cell Culture
C. elegans embryonic cells were cultured following published procedures (19,20). Briefly, gravid adults were cultured on enriched peptone plates (8P plates) seeded with Escherichia coli strain Na22. Eggs were isolated by lysing animals with a solution containing fresh bleach and 10 N NaOH. Eggs were separated from animal carcasses by treatment with 30% sucrose dissolved in egg buffer solution (118 mM NaCl, 48 mM KCl, 2 mM CaCl 2 , 2 mM MgCl 2 , 25 mM Hepes, pH 7.3, 340 mosM). The eggshell was removed by treatment with 2 mg/ml chitinase (Sigma Aldrich, catalogue number C6137), and cells were dissociated by manual pipetting through a 27-gauge needle, resuspended in culture medium (L-15 ϩ 10% FBS ϩ 45 mM sucrose ϩ 100 IU of penicillin and 100 g/ml streptomycin ) and plated on glass coverslips previously covered with peanut lectin (0.5 mg/ml). Cells were seeded at a density of ϳ230,000 cells/cm 2 and used 4 -6 days after seeding. Strains used for all microscopy were Is[mec- 10(d)] and nra-2(ok1731); Is[mec- 10(d)].

Immunofluorescence Staining and Microscopy
Fluorescent photographs of GFP C. elegans were taken using a Zeiss Axiovert 200m microscope equipped with a GFP filter and a QImaging Rolera e-mc 2 camera with MetaMorph software. For MEC-4::GFP and MEC-10:GFP quantification, photographs were taken using the same exposure time (300 ms), and quantifications were performed in ImageJ.
C. elegans primary embryonic cell culture was set up in 35-mm glass bottom cell culture dishes (Bioexpress, catalog number T-2881-16). Primary embryonic cells from C. elegans were fixed in 4% paraformaldehyde for 20 min and permeabilized for 10 min with M9 ϩ 0.1% Tween 20. Cells were blocked in 2% BSA for 1 h at room temperature, incubated with primary antibody for 1 h at room temperature, washed three times with M9 ϩ 0.01% Tween 20, incubated with secondary antibody for 1 h at room temperature, washed three times with M9 ϩ 0.01% Tween 20, and imaged on a Zeiss Axiovert microscope with a 63ϫ NA 1.3 objective. Images were taken at 250-ms exposure, at 1 ϫ 1 binning. Images were deconvolved using Autoquant X3. Measurements were made on thresholded images using MetaMorph software.
ER Tracker and LysoTracker Staining-C. elegans primary embryonic cell culture was set up as described above. ER tracker Blue White DPX (Life Technologies catalog number MP12353) or LysoTracker Blue DND-22 (Life Technologies catalog number L-7525) were incubated at 1 M concentration in M9 ϩ 0.01% Tween 20 for 30 min and washed three times with M9 ϩ 0.01% Tween 20 prior to imaging.

TIRF Microscopy
C. elegans primary embryonic cell culture was set up using Is[mec- 10(d)] and nra-2(ok1731); Is[mec-10(d)] strains as described above. Cells were imaged live on an Olympus IX70 microscope using an Olympus PlanApo 60ϫ NA 1.45 total internal reflection fluorescence microscopy (TIRFM) objective. Touch neurons in cell culture were identified by mCherry expression using standard wide-field microscopy. TIRF imaging was done using a Melles Griot Ar488 laser (Melles Griot Laser Group, Carlsbad, CA) and an Olympus IX2 Laser Combiner. A micrometer screw was used to change the incident beam angle to 61°for TIRF mode imaging. All images were taken at 500-ms exposure using iVision software (BioVision Technologies Inc., Exeter, PA). Image quantification was done in ImageJ. Images were thresholded, a region of interest was drawn around the cell body, and mean intensity of fluorescent signal was measured. Statistical analysis was performed using GraphPad Prism 6.

A Targeted RNAi Screen for Calcium-responsive Genes That
Modulate Channel-induced Necrosis Identifies ER Chaperone nra-2-To extend molecular understanding of necrosis mechanisms, we sought to identify modulators of genetically induced touch neuron degeneration. Because calcium is a key factor in neuronal necrosis across species (7,22,23), we selected a collection of 191 C. elegans genes that encode EF hand calciumbinding proteins for targeted knockdown by feeding RNAi (24). We first constructed a strain with weak necrosis inducer mec-10(d) (3) that co-expressed GFP in TRNs and is sensitive to RNAi in neurons (P mec-4 mec-10(d)::GFP; nre-1(hd20); lin-15B(hd126); screen strategy summarized in Fig. 1A). At 15°C we observed baseline PLM tail touch neuron degeneration of ϳ2%, as described previously (15). We then fed this strain either empty vector control (L4440) or dsRNA EF hand gene clones from the L1 to L4 larval stages and evaluated the extent of neuronal death by counting surviving fluorescent PLM tail TRNs. Of the 191 EF hand genes tested, RNAi directed against clone T05F1.1 (nra-2) exhibited the greatest enhancement of mec-10(d)-induced necrosis: a 3-fold increase as compared with vector control treatment.
Note that nra-2 encodes an ER-resident transmembrane protein homologous to mammalian nicalin (31% identity, 49% similarity). Both proteins contain an N-terminal aminopeptidase domain with poor conservation in the catalytic residues; this domain is not thought to possess peptidase activity. NRA-2 contains an EF hand domain within the aminopeptidase domain that is absent in mammalian nicalin (Fig. 1C).
To rule out any extraneous effect of RNAi on death enhancement, we constructed a nra-2(⌬); mec-10(d) double mutant strain. The double mutant exhibited over a 5-fold increase of mec-10(d)-induced TRN degeneration as compared with mec-10(d) alone (Fig. 1D). As further confirmation that nra-2 was the enhancer locus, we expressed the nra-2(ϩ) transgene in TRNs of nra-2(⌬); mec-10(d) animals and observed a 3-fold reduction of TRN degeneration as compared with control nra-2(⌬); mec-10(d) animals, i.e. a restoration of TRN degeneration to mec-10(d) levels (Fig. 1D). This transgene rescue both confirms that the nra-2 mutation confers the death-enhancing phenotype and establishes that nra-2 acts cell autonomously within the touch receptor neuron to modulate necrosis. Because nra-2(⌬) itself does not induce any TRN necrosis (Fig.  1D), our data establish that loss of nra-2 function results in increased necrosis due to mec-10(d)-induced insult.

NRA-2 Does Not Play an Essential Role in the Differential Distribution of MEC-10 and MEC-4 Subunits in Touch
Receptor Neurons-NRA-2 encodes a transmembrane ER-resident protein implicated in regulation of acetylcholine receptor (AChR) subunit composition in C. elegans muscle, possibly by allowing only specific subunit combinations of that receptor to exit the ER (16). This role in channel assembly raises the question as to whether NRA-2 might similarly regulate distribution, assembly, or surface expression of the touch-sensing MEC channel. The gentle touch-sensing MEC channel is proposed to be composed of MEC-4 and MEC-10 subunits (3,26,27). However, some data suggest that the MEC channel assembled in vivo on neuronal processes is primarily made up of MEC-4 subunits (28) and that the MEC channel at the cell body is heteromeric, containing MEC-4 and MEC-10 subunits. In support of this model, MEC-4::GFP localizes in puncta along the process length (28, 29) ( Fig. 2A), but MEC-10::GFP is localized at the cell body (28) (Fig. 2B). Consistent with localized functional roles, mec-4 null mutants are insensitive to touch delivered anywhere along the length of the process, but mec-10 null mutants are insensitive only to touch administered in the vicinity of the soma, and are otherwise sensitive to touch along the process. Thus, MEC-4 homomeric channels might be the primary transducers of touch along the process, but MEC-4/MEC-10 heteromers might function in sensing touch at the cell body (28).

NRA-2/Nicalin and Toxic DEG/ENaCs Localization
If NRA-2 plays a role in restricting MEC-4 and MEC-10 to different regions within the touch neuron, the distributions of MEC-4::GFP and/or MEC-10::GFP might be altered in the nra-2 mutant background. We constructed lines that were MEC-4::GFP; nra-2(⌬) and MEC-10::GFP; nra-2(⌬) and compared basic subunit distribution Ϯ nra-2. (Note that both translational fusion constructs have C-terminal tags and can functionally rescue cognate mutations (28,30).) Our comparison of distribution of tagged MEC-4 and MEC-10 proteins did not reveal any striking distribution changes Ϯ nra-2 (Fig. 2, A and  B). General level of channel expression as measured by fluorescence intensity also appeared similar Ϯ nra-2 (Fig. 2, C and D). Thus analysis of tagged reporters does not support a major role for NRA-2 in changing the distinctive distribution of MEC-4::GFP and MEC-10::GFP channel subunits or in regulating overall GFP-tagged subunit stabilities.
To address whether native subunits at physiological expression levels might be differentially affected by nra-2 deficiency, we scored touch sensitivity over the process length Ϯ nra-2 (primarily a score of MEC-4 function). We observed that the nra-2(⌬) mutants were only modestly touch-insensitive (Fig. 2E). More-over, when we touched in the vicinity of the cell body Ϯ nra-2, we noted only a slight change from 2 to 10% touch-insensitive in nra-2(⌬) (n ϭ 100, not significant by paired two-tailed t test). Taken together, our data support that most mechanotransducing complexes are distributed and function even when NRA-2 is lacking. These data suggest that NRA-2 might act preferentially on toxic rather than wild type DEG/ENaC subunits.

nra-2(⌬) Does Not Enhance mec-4(d)-induced Neuronal
Death-We identified nra-2(RNAi) as an enhancer of mec-10(d)-induced necrosis, but potential effects on the stronger necrosis inducer mec-4(d) were unknown. To test whether nra-2(⌬) can enhance mec-4(d)-induced neuronal death, we constructed nra-2(⌬); mec-4(d) double mutants and measured the extent of TRN death. As reported previously, mec-4(d) induces a high level of TRN degeneration (ϳ85% of cells die), in striking contrast to mec-10(d) (3,4). We found that the absence of nra-2 does not enhance neuronal death further than occurs in mec-4(d); nra-2(ϩ) (Fig. 2F). These data, together with localization and touch sensitivity data, are also consistent with a model in which NRA-2 does not play a major role in MEC-4 biology, although we cannot be certain of a "ceiling effect" for mec-4(d)- Nicalin, unlike NRA-2, does not contain an EF hand domain. There is 31% identity and 49% similarity between the two proteins. Domain predictions were made using the ScanProsite Tool (available from ExPASy). D, nra-2 deletion enhances mec-10(d)-induced necrosis. We scored the percentage of degeneration as in B. nra-2(⌬) is nra-2(ok1731); rescue transgene is Ex[P mec-4 nra-2; P ttx-3 RFP] Error bars, S.E.; average of three repeat trials (each with at least 30 L4 animals), **, p ϭ 0.0033, one-way ANOVA. APRIL 25, 2014 • VOLUME 289 • NUMBER 17 induced necrosis in which higher enhanced levels of neuronal death are not possible. Our findings raise the possibility that NRA-2 might interact specifically with only the MEC-10 or MEC-10(d) subunits in vivo.

MEC-10(d) Concentration in the ER Compartment Is
Diminished When nra-2 Is Absent-Toxicity of the MEC-4(d) channel subunit is associated with enhanced channel activity (6 -8, 31), and thus enhanced MEC-10(d) channel activity is a plausible mechanism for increased cell death. Given that NRA-2 is an ER protein involved in AChR channel maturation, we wondered whether NRA-2 acts in the ER to limit surface expression of the MEC-10(d) channel. To address this hypothesis, we first monitored subcellular distribution of MEC-10(d)::GFP in various intracellular compartments, Ϯ nra-2. We elected to work with cultured C. elegans touch neurons (20,32), which allowed us greater freedom to use immunohistochemical and imaging techniques that are impractical in vivo. We measured colocalization of MEC-10(d)::GFP with fluorophore-labeled antibodies for endosome (EEA-1) and Golgi body (mAbSQV8), and we dye-labeled lysosomes and ER in cultured TRNs of nra-2(⌬);mec-10(d)::GFP and control mec-10(d)::GFP animals (Fig. 3, A-H). Mean MEC-10(d)::GFP colocalization with endosomes, lysosomes, or Golgi bodies did not significantly differ between nra-2(⌬) and control animals. Mean ER colocalization of the MEC-10(d)::GFP and ER, however, was ϳ 25% for nra-2 mutants as compared with 50% for control mec-10(d) animals (Fig. 3I). We compared total ER-associated immunofluorescence for control and mutant strains and found no significant difference in levels, suggesting that the decrease in MEC-10(d)::GFP fluorescence in nra-2 mutants is not due to a general decrease in the volume of the ER (Fig. 3J). Our data suggest that at the intracellular level, MEC-10(d)::GFP is localized primarily to the ER, and that the absence of nra-2 reduces ER-associated MEC-10(d)::GFP.  (33). The resultant low intensity evanescent wavelets penetrate ϳ150 nm into the medium and thereby excite fluorescent proteins at or near the plasma membrane. TIRFM thus allows live imaging of plasma membrane-associated proteins with high X-Y resolution and very low noise.
Our TIRFM imaging revealed a striking concentration of surface MEC-10(d)::GFP at a site near the nucleus, either with or without nra-2. The biological reason for this highly restricted distribution of MEC-10(d)::GFP is unclear, but might underlie its role in touch perception near the cell body (see "Discussion"). Importantly, our TIRFM studies showed that mean fluorescence intensity of surface-localized MEC-10(d)::GFP was significantly increased in the nra-2(⌬) background (p Ͻ 0.0001, ϳ1200 units control, ϳ2400 units nra-2(⌬) ; Fig. 4, A  and B). Furthermore, we normalized the mean intensity of surface fluorescence to the mean surface area of the cell body for each strain and observed a significant increase in normalized surface fluorescence in the nra-2(⌬) background (Fig. 4C). These data suggest that loss of nra-2 function results in an increase of surface-associated MEC-10(d)::GFP. Coupled with data supporting decreased ER-associated MEC-10(d)::GFP in nra-2(⌬), our data suggest a role for NRA-2 in ER retention of MEC-10(d). The increased MEC-10(d) surface expression in

NRA-2/Nicalin and Toxic DEG/ENaCs Localization
the nra-2(⌬) background, accompanied by the expected increased mutant channel ion influx, could be the mechanistic basis for the enhanced neurotoxicity.

NRA-2 limits MEC Channel Currents in an Oocyte
Expression System-Co-expression of MEC-4(d), MEC-2, and MEC-6 in Xenopus oocytes generates an amiloride-sensitive channel with a large inward Na ϩ current as well as a Ca 2ϩ -activated Cl Ϫ current that reveals inward Ca 2ϩ conductance (6 -8). MEC-4(d) is essential for the generation of both currents and, interestingly, the addition of MEC-10(d) diminishes these currents (6 -8, 34). Our in vivo and cell culture data suggest that enhanced necrosis in TRNs of nra-2 mutants may be attributed to increased ion influx due to increased surface expression of MEC-10(d) channels. We tested this model by measuring Na ϩ current in oocytes expressing MEC-4, MEC-10(d), MEC-2, and MEC-6 (henceforth described as the MEC-10(d) complex), with or without NRA-2 (Fig. 5, A and B). We found that MEC-10(d) complex-expressing oocytes showed a mean inward current of 1.5 A. The addition of NRA-2 to the injected MEC-10(d) subunits reduced mean inward current to 0.15 A. That co-expression of NRA-2 with the MEC-10(d) complex suppressed Na ϩ current by ϳ90% is consistent with a negative role for NRA-2 in MEC-10(d) currents (Fig. 5C).
We wondered whether the Na ϩ current suppression was due to a MEC-10(d)-specific interaction with NRA-2, as suggested by genetic studies. Surprisingly, however, we found that the addition of NRA-2 could also suppress currents in oocytes expressing only MEC-4 and MEC-4(d) (p Ͻ 0.05; Fig. 5D), indicating that the MEC-10(d) subunit is not essential for NRA-2 impact. Notably, combinations that included MEC-4(d) without native MEC-4(ϩ) were not susceptible to NRA-2-induced current suppression, with either MEC-10(ϩ) or MEC-10(d) coexpressed (Fig. 5D). These data support the in vivo scoring of neuronal death Ϯ nra-2 in mec-4(d) mutants (Fig. 2F) and suggest that a MEC-4(ϩ) subunit might be needed for NRA-2 impact on channel surface expression or that a channel composed of a specific number of mutant versus wild type subunits is impacted by NRA-2. Note that a limitation of our assay is that it is not possible to address whether M4/M10 channels or channels composed of M10d only could be suppressed because no current is detectable with these combinations (6,8).
In sum, from our electrophysiological studies in the oocyte expression system, we underscore three observations: (i) NRA-2 limits currents for the co-expressed MEC-4, MEC-10(d), MEC-6, MEC-2 channel; (ii) the presence of at least one MEC-4 appears essential to allow NRA-2 current suppression in a channel complex containing either MEC-4(d) or MEC-10(d); and (iii) NRA-2 current suppression does not require the presence of either MEC-10 or MEC-10(d). These results suggest that the mechanism of NRA-2 action may not be MEC-10(d)-specific, but rather might involve a more complex capacity of NRA-2 to sense the correct conformation of an assembled, or partially assembled, channel complex that involves more than one MEC subunit. A Model for NRA-2 Influence on MEC-10(d) Neurotoxicity-Our data suggest that NRA-2 acts in the ER to mediate surface expression of properly folded/assembled MEC-4/MEC-10 channels (summary in Fig. 6). Correctly folded heteromers traffic to the plasma membrane. When the aberrantly folded MEC-10(d) or MEC-4(d) subunit is present, the specific three-dimensional conformation of the assembling channel (which includes MEC-4) is sensed as being abnormal, and NRA-2 preferentially retains those channels in the ER. In the absence of NRA-2, more channels that contain aberrant subunits escape quality control in the ER, which creates more leaky channels at the cell membrane, leading to enhanced neurotoxicity.

DISCUSSION
Our studies document the role of ER-resident chaperone protein NRA-2 in modulation of MEC-10(d) channel-induced necrosis in C. elegans. Combining genetic approaches, live imaging, immunohistochemistry, and electrophysiological assays in Xenopus oocytes, we show that loss of nra-2 function results in an increase in the surface expression of toxic MEC-10(d) channels to enhance TRN necrosis. Conversely, NRA-2 limits channel activity in heterologous expression assays. We propose that NRA-2 acts in the ER to distinguish specific conformations of assembling channel complexes, restricting exit of immature or malformed channels. Our work underscores the theme of enhanced channel activity in necrosis induction, extends understanding of NRA-2 function in channel biogenesis, and suggests a potential in vivo molecular strategy for increasing/decreasing surface expression of DEG/ENaC subunits that might be of therapeutic value in some human disease states.
Increased MEC-10(d) Channel Surface Expression Is Associated with Enhanced Neurotoxicity-Previous work has supported that increased activity of mutant DEG/ENaC channels and their associated Na ϩ and Ca ϩ2 influx initiates neuronal necrosis (4,7,31,35). Here we have shown that nra-2(RNAi) and nra-2 deletion enhance mec-10(d)-dependent neuronal loss via a cell-autonomous mechanism that increases MEC-10(d) surface expression. In the absence of NRA-2, more MEC-10(d) channels reach the neuronal surface, where they collectively conduct higher cation influx than normal, enhancing neurotoxicity. Thus our data reinforce the idea that increased channel activity, either induced via subunit abnormality or induced by increased numbers of plasma membrane channels, is a necrosis-initiating insult.
An Unusual Subcellular Localization of MEC-10(d)::GFP Subunits-Our TIRFM studies highlighted a striking and unexpected concentration of surface-expressed MEC-10(d)::GFP to a highly localized spot at the periphery of the TRN soma. The MEC-10(d)::GFP is not concentrated near lysosomal compartments, but rather is more prevalently partitioned between the ER and the cell surface domain. Neurotoxic outcomes support that MEC-10(d) is a component of an active channel. The concentrated MEC-10::GFP distribution contrasts strongly with the punctate distribution of MEC-4::GFP subunits in the process thought to correspond to gentle touch-transducing touch complexes (29). The dramatic difference in distribution is consistent with previous studies indicating that MEC-4 is needed for response to touch stimulation all along the neuronal process, whereas MEC-10 is needed for responses to touch stimuli only near the soma (28). Restriction of DEG/ENaCs to specific raft domains is known for both invertebrate and mammalian members of this channel class (29, 36 -38). Interestingly, studies of ␤NaC distribution in mouse C-fiber small diameter neurons suggest a soma rather than process distribution (surface expression not assayed) (39), and neuronal ASIC1a has been observed to be largely ER-concentrated, with regulated surface expression under conditions in which ASIC2a is not similarly distributed (40), suggesting differential trafficking and localization as a feature of the channel class shared across phyla. Still, why a distinct MEC-10 channel type might be needed in a focused cellular domain in the soma is not clear. One possibility is that the MEC-10-enriched domain corresponds to a specialized mechanosensory site. Alternatively, this cellular domain might constitute a holding site for end-stage MEC channel sur-face assembly prior to transport down the process. MEC-10, which can negatively regulate channel activity (8,34), might be held and added to the MEC complex only under specific physiological conditions, or might even provide a chaperone function. The function of this MEC-10-concentrating cellular domain clearly remains to be elucidated. Because MEC-10 is plays a minor role in gentle touch, a novel activity could be anticipated.
Expanding Understanding of NRA-2 Function-NRA-2 was originally identified as a protein that co-immunoprecipitated with a muscle-specific C. elegans acetylcholine receptor (41). Later studies revealed a role for nra-2 in AChR subunit selection and/or surface expression in muscle (16). More specifically, this study showed that specific AChR subunits co-expressed in muscle were selected against being included into drug-sensitive AChR complexes when NRA-2 was active (16). Similarly, our work shows that NRA-2 functions in ER retention of channels containing mutant MEC-10 subunits. The mild effect of nra-2(⌬) on mec-4-based touch sensitivity could be consistent with an increased (but low level) inclusion of the channel-inhibiting MEC-10 subunit in the MEC-4 channels or more inappropriately folded complexes assembling, although more broad effects on other channels and neuron physiology cannot be ruled out. An effect linked to NRA-2 Ca 2ϩ binding capability could also be operative. Interestingly, however, NRA-2 does not impact mec-4(d)-induced neurodegeneration, suggesting that NRA-2 might recognize only specific channel compositions. Importantly, the oocyte experiments support that NRA-2 does not need the presence of MEC-10 in the channel complex to exert its effects, but it can also act on channel complexes made of wild type and mutant MEC-4 (but not made of MEC-4 mutant only). Taken together, our data suggest that NRA-2 recognizes and selects protein complex to exit the ER, likely based on the integrity of their tertiary/quaternary structure. Because ER calcium contributes to folding efficacy and conformational evaluation of forming complexes, the implication of calcium-binding EF hand protein NRA-2 in MEC complex quality control raises the possibility that the EF hand domain contributes to efficiency of assembly.
An NRA-2-related Complex Might Influence Channel Biogenesis across Species-It is noteworthy that reported regulated surface expression of mammalian channel ASIC1a shares striking features with the MEC-10(d) data we observe: concentration in ER at a site surrounding the nucleus and differential trafficking of the ASIC1a subunit (for ASIC1a, under serum or insulin deprivation, but co-expressed ASIC2a is not impacted) to enhance channel activity (40). Regulated ER retention may thus underlie differential DEG/ENaC subunit expression across species.
NRA-2 is a resident ER protein that is most homologous to human nicalin and more distantly related to the nicastrin subunit of the mammalian ␥-secretase complex. Nicastrin can promote maturation and trafficking of other proteins in the ␥-secretase complex and has been suggested to act as a nucleation center to regulate inclusion of specific components of the complex (42). Mammalian nicalin works in a complex with NOMO (43) and TMEM147 (44) and can modulate Nodal/ TGF␤ growth factor signaling in Zebrafish (43) via an unclear mechanism that does not appear to involve nicalin protease activity.
Human nicalin can partially rescue nra-2 defects in C. elegans muscle, and NOMO-homologous NRA-4 co-immunoprecipitations along with NRA-2 when AChR is targeted (16). Moreover, nra-4 mutants have similar defects to nra-2 mutants and to nra-2; nra-4 double mutants in C. elegans muscle, supporting roles in the same process and activity in the same complex. Considering structural and functional similarities along with our clear demonstration of NRA-2 influence on MEC-10(d) surface expression, we speculate that the mammalian Nicalin/NOMO complex might also influence DEG/ENaC surface expression (and possibly other channels). If so, this step might be considered for therapeutic modulation of DEG/ENaC channel activity in cystic fibrosis (45), renal disease (46), pain management, and possibly ischemic stroke (21).