The 68-kDa Telomeric Repeat Binding Factor 1 (TRF1)-associated Protein (TAP68) Interacts with and Recruits TRF1 to the Spindle Pole during Mitosis*

Background: The significance of telomeric protein translocation to the spindle pore is unknown. Results: TAP68 mediates TRF1 transit to the centrosome in mitotic cells. Conclusion: Repression of TAP68 induces genomic instability possibly through inhibition of TRF1 and tankyrase recruitment to centrosomes. Significance: Identifying TRF1-interacting proteins that regulate its localization is crucial for understanding the role of telomeric proteins in mitosis. The telomere capping protein TRF1 is a component of the multiprotein complex “shelterin,” which organizes the telomere into a high order structure. Besides telomere maintenance, telomere-associated proteins also have nontelomeric functions. For example, tankyrase 1 and TRF1 are required for the maintenance of faithful mitotic progression. However, the functional relevance of their centrosomal localization has not been established. Here, we report the identification of a TRF1-binding protein, TAP68, that interacts with TRF1 in mitotic cells. TAP68 contains two coiled-coil domains and a structural maintenance of chromosome motifs and co-localizes with TRF1 to telomeres during interphase. Immediately after nuclear envelope breakdown, TAP68 translocates toward the spindle poles followed by TRF1. Dissociation of TAP68 from the telomere is concurrent with the Nek2A-dependent phosphorylation at Thr-221. Biochemical characterization demonstrated that the first coiled-coil domain of TAP68 binds and recruits TRF1 to the centrosome. Inhibition of TAP68 expression by siRNA blocked the localization of TRF1 and tankyrase 1 to the centrosome. Furthermore, siRNA-mediated depletion of TAP68 perturbed faithful chromosome segregation and genomic stability. These findings suggest that TAP68 functions in mediating TRF1-tankyrase 1 localization to the centrosome and in mitotic regulation.

The telomere capping protein TRF1 is a component of the multiprotein complex "shelterin," which organizes the telomere into a high order structure. Besides telomere maintenance, telomere-associated proteins also have nontelomeric functions. For example, tankyrase 1 and TRF1 are required for the maintenance of faithful mitotic progression. However, the functional relevance of their centrosomal localization has not been established. Here, we report the identification of a TRF1-binding protein, TAP68, that interacts with TRF1 in mitotic cells. TAP68 contains two coiled-coil domains and a structural maintenance of chromosome motifs and co-localizes with TRF1 to telomeres during interphase. Immediately after nuclear envelope breakdown, TAP68 translocates toward the spindle poles followed by TRF1. Dissociation of TAP68 from the telomere is concurrent with the Nek2A-dependent phosphorylation at Thr-221. Biochemical characterization demonstrated that the first coiled-coil domain of TAP68 binds and recruits TRF1 to the centrosome. Inhibition of TAP68 expression by siRNA blocked the localization of TRF1 and tankyrase 1 to the centrosome. Furthermore, siRNA-mediated depletion of TAP68 perturbed faithful chromosome segregation and genomic stability. These findings suggest that TAP68 functions in mediating TRF1-tankyrase 1 localization to the centrosome and in mitotic regulation.
Telomeres, which are specialized nucleoprotein complexes at the ends of eukaryotic chromosomes that consist of tandem repeated DNA sequences bound by various telomeric proteins, prevent chromosomal termini from undergoing unregulated degradation, recombination, and fusion (1)(2)(3). From budding yeast to human cells, duplex telomeric DNA repeat-binding factors and their interacting proteins have been identified and characterized (4 -6). Although significant divergence has occurred in these proteins across species, there are also extensive levels of conservation. For example, in fission yeast, duplex telomeric DNA repeats are bound by Taz1p, a homologue of the human telomeric repeat binding factor proteins (7,8). Like Taz1p, human TRF1 directly binds duplex telomere repeats and interacts with multiple proteins, including TERF1-interacting nuclear factor 2 (TIN2), tankyrase, and PIN2/TERF1interacting telomerase inhibitor 1 (PINX1) (6, 8 -11). Interestingly, the chromatin association of TRF1 is regulated by key mitotic kinase PLK1 during mitosis (12,13). Two related poly-(ADP)-ribose polymerases that were originally identified as TRF1-interacting proteins, tankyrase 1 and 2, inhibit the binding of TRF1 to telomeres (14). Tankyrase 1 and 2 are nearly identical in amino acid sequence and form homo-and heterodimers, suggesting that they are functionally similar (15).
In addition to their telomeric functions, telomere-associated proteins also have nontelomeric functions. For example, TRF1 expression correlates with stem cell pluripotency (16). The functional involvement of TRF1 in mitosis is well established. The spindle pole localization of TRF1 has been reported more than 10 years ago (4,17). Later, it was reported that TRF1 interacts with spindle assembly checkpoint protein Mad1 and key mitotic kinase NEK2A (NIMA-related kinase 2) (18,19). Recently, accumulating evidence suggests that TRF1 is required for faithful mitotic progression (20 -22). In addition, PinX1 phosphorylation has been associated with the regulation of faithful chromosome segregation (23,24). Tankyrase localizes to mitotic spindle poles during mitosis and is required for normal spindle formation and sister chromatid segregation through as yet unclear mechanisms (25). In addition, poly-(ADP-ribose) is required for spindle assembly and structure (26,27). Similar to TRF1, the function of tankyrase 1 is also regulated by PLK1 (Polo-like kinase 1)-mediated phosphorylation (28).
Centrosome is the major microtubule organization center and is essential for the correct assembly of bipolar spindle in animal cells. Aberrations in the number and structure of the centrosome lead to genomic instability and have been characterized in a variety of solid tumors as well as leukemias and lymphomas (29,30). Although the mitotic role of TRF1 is well established, the mechanism by which TRF1 and its complex is recruited and tethered to the centrosome in vertebrates is unknown. In this study, we aimed to assess the mechanism by which the telomere-associated proteins regulate chromosome stability using a proteomic analysis to isolate TRF1-interacting proteins during mitosis. A protein of unknown function was identified and was designated as TAP68 (TRF1-associated protein of 68 kDa). Biochemical analysis demonstrated the direct interaction between TRF1 and TAP68. These two proteins colocalize on telomere in interphase and at mitotic spindle poles in mitosis. The spindle pole localization of TRF1 depends on TAP68. We further demonstrated that phosphorylation of TAP68 at residues Thr-221 and Thr-457 by kinase NEK2A and PLK1 coordinates TRF1 translocation from telomere to spindle pole. Together, our data reveal TAP68 plays a regulatory role in TRF1 localization to centrosomes and uncover the novel mechanism of telomeric protein in the regulation of accurate mitotic progression.
Preparation of Samples for Mass Spectrometry-In-gel digestions were done essentially as described previously (31,32). Briefly, the protein band, P1, was excised and subjected to digestion by modified porcine trypsin (50 -100 ng/digestion; Promega). Peptides were recovered, co-crystallized with cyano-4-hydroxycinnamic acid, and analyzed using MALDI-TOF mass spectrometry (Voyager DE-STR, Perspective Biosystems) equipped with a nitrogen laser. All MALDI spectra were externally calibrated using a standard peptide mixture (Sigma). Some post-source decay spectra were acquired on a TofSpec SE MALDI-TOF mass spectrometer (Micromass, Manchester, UK). Database interrogations based on experimentally determined peptide masses were carried out using mass spectrometry (MS)-Fit, and post-source decay data interrogation was performed using MS-Tag as described previously (31).
Molecular Cloning and Plasmid Construction-The fulllength TRF1 mRNA was amplified from the human tonsil tissue by retrotranscriptase (RT)-PCR with the following primers: sense, 5Ј-CGGGTACCTTAACATGGCGGAGGATGTTT-CCT-3Ј, and antisense, 5Ј-CCGGAATTCAATACTTAACTG-TCCTTTCATCAA-3Ј. The thermo-cycling conditions were as follows: 1 cycle at 95°C for 3 min; 30 cycles at 94°C for 45 s, 52°C for 30 s, and 72°C for 90 s; and 1 cycle at 72°C for 10 min. The resulting product was introduced into the pCR-II TOPO vector for DNA sequencing. After confirmation by sequencing, the coding sequence of TRF1 was amplified by PCR with the EcoRI-containing forward primer 5Ј-CGGAATTCCGGGCG-GAGGATGTTTCC-3Ј and the BamHI-containing reverse primer 5Ј-CGGGATCCGCGGTCTTCGCTGTCTGA-3Ј, using the pCR-II TOPO/TRF1 construct as template. The thermocycling conditions were as follows: 1 cycle at 95°C for 3 min; 30 cycles at 94°C for 45 s, 50°C for 30 s, and 72°C for 90 s; and 1 cycle at 72°C for 10 min. The PCR product was then subcloned into the following vectors for in vivo and in vitro expression: pEGFP-C2, p3ϫFLAG-myc-CMV-24 (Sigma), and pET28a (Novagen). The coding region of TAP68 was isolated from a human testis cDNA library (BD Biosciences) using temperature-gradient PCR with the following primers: sense, 5Ј-CCC-GATCTGCTCAACTTCAA-3Ј, and antisense, 5Ј-CTACTCA-GCCAGGCTGTTGC-3Ј. The thermo-cycling conditions were as follows: 1 cycle at 95°C for 3 min; 30 cycles at 94°C for 45 s, 50 -62°C for 30 s, and 72°C for 90 s; and 1 cycle at 72°C for 10 min. The candidate fragment was cloned into the pMD18-T vector (Takara Biotechnology, Dalian, China) and completely sequenced. The clone with the correct insert was utilized as the template for PCR amplification with the high fidelity DNA polymerase, Pyrobest (Takara Biotechnology), and the target fragment was inserted into pEGFP-C2 by blunt-end cloning (BD Biosciences). All plasmid constructs were sequenced for verification. Site-directed mutagenesis was carried using a standard PCR technique. Both TRF1 and TAP68 coding regions were found to be identical to the sequences detected in NCBI GenBank TM (accession numbers U40705 and NM_007032, respectively).
In Vitro Kinase Assay-Both the His-tagged wild-type and phosphomutant TAP68 proteins were expressed in Escherichia coli BL21(DE3)pLys and purified as described previously (34). The fusion proteins bound to nickel beads were suspended in phosphorylation buffer (25 mM HEPES, pH 7.5, 5 mM MgSO 4 , 50 mM NaCl, 2 mM EGTA) prior to use. To verify whether Thr-221 is a substrate for NEK2A or Thr-457 is a substrate for PLK1, 2 g of purified His-TAP68 fusion proteins, both wild-type and mutants, were incubated with 1 unit of active Nek2A or PLK1 (Upstate Biotechnology, Lake Placid, NY) in kinase buffer containing 20 mM HEPES, pH 7.5, 10 mM MgCl 2 , 5 mM EGTA, 1 mM dithiothreitol, 10 M ATP, and 5 Ci of [ 32 P]ATP (PerkinElmer Life Sciences) in a total volume of 30 l. Kinase reactions were carried out at 30°C for 30 min and terminated by the addition of 10 l of 4ϫ SDS-PAGE sample buffer and separated by 6 -16% gradient SDS-PAGE. The gel was stained with Coomassie Brilliant Blue, dried, and quantified using a PhosphorImager (GE Healthcare).
Immunofluorescence Microscopy-For immunofluorescence, HeLa cells were grown onto sterile, acid-treated 18-mm coverslips in 24-well plates (Corning Glass, Corning, NY) and maintained in DMEM with 10% FBS until nearly 80% confluence. Cells were rinsed for 1 min with PHEM buffer (100 mM PIPES, 20 mM HEPES, pH 6.9, 5 mM EGTA, 2 mM MgCl 2 , and 4 M glycerol) and permeabilized for 1 min with PHEM plus 0.2% Triton X-100 as described previously (33,35). Extracted cells were then fixed in freshly prepared 4% paraformaldehyde plus 0.05% glutaraldehyde in PHEM and rinsed three times in PBS. After the cells were blocked with 0.05% Tween 20 in PBS (TPBS) containing 1% BSA, they were incubated with various primary antibodies in a humidified chamber for 1 h followed by three washes to remove unbound antibody. Monoclonal antibodies bound to ␥-tubulin and ␤-tubulin were visualized using rhodamine-conjugated goat anti-mouse IgG (Molecular Probes, Eugene, OR). DNA was stained with 4,6-diamidino-2-phenylindole (DAPI, Sigma). Slides were examined with an Axiovert-200 fluorescence microscope (Carl Zeiss, Göttingen, Germany) with a ϫ63 1.40 NA PlanApo objective, and the images were captured by AxioVision 3.0 software and analyzed with Image-5 (Carl Zeiss).
Co-immunoprecipitation Assay-For co-immunoprecipitation experiments, the FLAG-tagged TRF1 construct was cotransfected with GFP-tagged TAP68 constructs or GFP vector alone (20 g/dish) into human 293T cells using calcium phosphate. After 4 h, fresh medium was added. The cells were detached from the dishes by flushing with cold PBS 36 h after transfection, harvested by centrifugation, and lysed in pre-cooled lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, and 1% Triton X-100, 1 mM dithiothreitol, 2 mM phenylmethylsulfonyl fluoride, 1 g/ml aprotinin, 1 g/ml pepstatin A, and 1 g/ml leupeptin). After incubation for 30 min on a shaker at 4°C, detergent-insoluble debris was removed by centrifugation at 14,000 ϫ g at 4°C for 15 min. Cell lysates were further treated with -phosphatase (New England Biolabs) for 30 min at 30°C after incubation and centrifugation at 14,000 rpm at 4°C for 15 min. Cleared lysates were transferred to a new tube and incubated on a rocking platform at 4°C for 4 h with either 10 l of FLAG-M2-coupled agarose beads or 10 l of protein A/G coupled with 10 g of anti-GFP antibody. After the beads were washed three times with lysis buffer and two times with PBS, proteins were eluted with 50 l of Laemmli loading buffer and resolved by 10% SDS-PAGE. Separated proteins were subsequently transferred onto nitrocellulose membrane for Western blot analysis with either GFP or FLAG monoclonal antibodies.
TAP68 Knockdown-The siRNA sequence used for silencing TAP68 corresponds to the coding region 336 -354 (relative to the start codon). As a control, either a duplex targeting cyclophilin or scrambled sequence was used (36). The 21-mer oligonucleotide RNA duplexes were synthesized by Dharmacon Research, Inc. (Boulder, CO). Double thymidine synchronized cells were transfected with siRNA oligonucleotides using Oligofectamine (Invitrogen). The efficiency of the siRNA oligonucleotide on TAP68 protein knockdown was assessed by Western blot analysis after 48 -72 h.
Subcellular Fractionation-2 ϫ 10 6 interphase HeLa cells were used and incubated with 10ϫ v/v of microtubule-stabilizing buffer (PEM buffer) solutions, which contained 5 mM PIPES, pH 7.2, 0.5 mM EDTA, 5 mM MgCl 2 , 5 mM NaCl, 1 mM PMSF, 2 mg/ml aprotinin, 2 mg/ml pepstatin A, 2 mg/ml leupeptin, at room temperature for 5 min and centrifuged at 2500 ϫ g for 2 min to collect the cells. Cell pellets were re-dissolved in pre-cold 4°C hypotonic solutions (PEM buffer plus 0.1% digitonin) (Sigma) and sonicated for four bursts at setting level 2 for 5 s each in a 4°C ice bath with a BILON92 sonicator (Shanghai Bilon Instruments Co. Ltd., Shanghai, China) and sonicated on ice for four bursts of 5 s each by using a probe-tip sonicator BILON92. The homogenates were further centrifuged at 4°C, 15,000 ϫ g for 10 min. The supernatants (cytoplasm portion) and cell pellets (nuclei portion) were transferred separately to pre-cooling Eppendorf tubes and ready for further Western blot assay.
Immunoelectron Microscopy-For immunoelectron microscopy, HeLa cells were grown onto sterile, acid-treated 18-mm coverslips in 6-well plates (Corning Glass, Corning, NY) and maintained in DMEM with 10% FBS until nearly 75% confluent. After removing the medium, cells were washed for 1 min with PHEM buffer (100 mM PIPES, 20 mM HEPES, pH 6.9, 5 mM EGTA, 2 mM MgCl 2 , 4 M glycerol) and then incubated with PHEM buffer containing 1% digitonin to perforate the cell membrane for 5 min at room temperature. The extracted cells were pre-fixed in a freshly prepared 4% paraformaldehyde and 0.05% glutaraldehyde PHEM buffer for 10 min and rinsed with PBS three times for 5 min each. After blocking with PBST (0.05% Tween 20 in phosphate saline buffer, PBS) containing 1% BSA, cells were incubated with primary anti-TAP68 antibody at room temperature for 1 h. After washing three times with PBST for 3 min each, cells were incubated with colloidal gold particles conjugated with secondary antibody at room temperature for 1 h. Cells were fixed with 1% glutaraldehyde in PBS for 20 min and washed three times again with PBS. These cells were then treated with 2% osmium tetroxide for 30 min and dehydrated through graded ethanols (10,20,40,60, and 80% and two times at 100%, 5 min in each) and 100% acetone (three times) over 10 min. Cells were then detached from the coverslips and embedded in acetone-Epon 812 (Sigma) overnight at 30°C. After ultrathin sections were prepared, they were stained with lead acetate uranium and examined and photographed using a transmission electron microscope (JEOL 1200, Peabody, MA).
Statistical Analysis-All experiments were performed at least three times or more. The percentages of mitotic cells in bipolar, tripolar, and tetrapolar spindles were presented as means Ϯ S.D. The independent t test was performed to compare the differences of percentage of mitotic cells in bipolar, tripolar, and tetrapolar spindles between the cells treated with TAP68 siRNA and control siRNA. Statistical analysis was considered significant when the two-sided p value was Ͻ0.05, using SPSS 15.0 statistics software (SPSS Inc., Chicago).

Identification of a Novel TRF1-binding Protein, TAP68-To
further understand the role of TRF1 in mitotic regulation, soluble TRF1 and its potential binding partners were immunoprecipitated with an anti-TRF1 antibody from the lysates of mitotic HeLa cells because the TRF1-tankyrase 1 complex undergoes dynamic redistribution from the telomere to the centrosome upon the onset of mitosis (17). Mass spectrometric analysis of the immunoprecipitates showed that it contained several known telomere regulators, including tankyrase 1, TRF2, as well as a polypeptide (estimated mass of 68,000 Da) marked as P1 (Fig. 1A). The protein band of P1 was extracted from the acrylamide gel and digested with trypsin, and the resulting peptide fragments were subjected to mass spectrometric analysis (Fig. 1B). As shown in Fig. 1C, the six peptide sequences obtained matched the previously characterized open reading frame, AAH03618, that encodes a 593-amino acid protein with a predicted molecular mass of 68,041 Da. Computational analysis showed that AAH03618 contains one putative pleckstrin homology domain in its N terminus with two extended coiledcoil domains in its C terminus (Fig. 1D). In addition, AAH03618 contains a structural maintenance of a chromosome-like domain found in chromosome segregation-related ATPases (NCBI, www.ncbi.nlm.nih.gov) (26). Because the AAH03618 sequence distinguishes it from other TRF1-binding proteins, such as EB1 that localizes to the microtubule plus-end, we therefore referred to this protein as TAP68. The specific TRF1-TAP68 interaction was confirmed by using another anti-TRF1 antibody in high stringency lysis buffer containing 1% Nonidet P-40 and 0.5% deoxycholate. Under this condition, TAP68 remained firmly bound to TRF1, and the majority of other TRF1-binding proteins, such as TRF2 and tankyrase, were absent from the immunoprecipitate (Fig. 1E).
TAP68 Binds to TRF1 via Its Coiled-coil Domains-To further validate the interaction between TRF1 and TAP68, reciprocal co-immunoprecipitation was performed. First, we used anti-FLAG antibody to immunoprecipitate soluble TRF1 and its binding proteins from the lysates of mitotic HeLa cells transiently transfected to express FLAG-tagged TRF1 and GFPtagged TAP68. Western blot analysis with anti-GFP antibody validated that TAP68 co-precipitated with TRF1 ( Fig. 2A). No GFP was recovered in the FLAG immunoprecipitates, suggesting that the interaction between TRF1 and TAP68 is specific and independent of the GFP tag. Immunoprecipitation of TAP68 using an anti-GFP antibody confirmed the interaction between TAP68 and TRF1 (Fig. 2B).
To map the region(s) of TAP68, which binds to TRF1, we immunoprecipitated soluble TRF1 and its binding proteins from lysates of mitotic HeLa cells exogenously expressing fulllength and different deletion mutants of GFP-tagged TAP68 as shown in Fig. 2C. Western blot analysis with an anti-GFP antibody validated that the C-terminal 261 amino acids of TAP68 (TAP68-C) co-precipitated with TRF1 (Fig. 2D). But the TAP68⌬C that lacks the second coiled-coil domain can also bind TRF1. Thus, we concluded that both coiled-coil domains located on the C terminus of TAP68 were required for TRF1 binding. However, with assistance of the N-terminal domain, TAP68⌬C without the second coiled coil was able to bind TRF1.
TAP68 Co-localizes with TRF1 at the Telomeres in Interphase and Translocates to the Centrosome during Mitosis-TRF1 is localized within telomeric nuclear speckles during interphase and translocates to the mitotic spindle during mitosis (17). To examine whether TAP68 is a nuclear protein, interphase HeLa cells were homogenized and separated into nuclear and postnuclear fractions, and subcellular fractions were analyzed by Western blot using an anti-TAP68 mouse antibody. As shown in Fig. 3A (left and middle panels), both TAP68 and TRF1 were highly enriched in the nuclear fraction.
To ascertain the subcellular localization of TAP68 relative to TRF1, HeLa cells were next stained with a TAP68 mouse antibody followed by a rhodamine-conjugated secondary antibody (red), and TRF1 was labeled with a rabbit antibody followed by a fluorescein-conjugated secondary antibody (Fig. 3B, green). In addition, a Cy5-conjugated ␥-tubulin mouse antibody was used to mark the centrosome (Fig. 3B, white, arrowhead; right panels). A pre-extraction procedure allows the effective labeling of the kinetochore and centrosomal proteins while preserving the fine cyto-structure of mitotic cells was also employed (33,35). In the interphase cells shown in Fig. 3B (panels a-d), TAP68 staining appeared as 21 clearly resolved dots and speckles in the nucleus (red and arrows), whereas TRF1 staining showed typical telomeric nuclear speckles (green and arrows). Merged images confirmed that TAP68 is a putative telomeric protein at interphase.

TAP68-TRF1 Interaction at Telomeres and the Spindle Pole
remained associated with condensing chromosomes without apparent centrosomal localization (Fig. 3B, panel h).
During metaphase and anaphase, TAP68 was fully relocated to the centrosome (Fig. 3B, panel j). Labeling with a TRF1 rabbit antibody revealed typical spindle pole staining in metaphase and anaphase cells, which was consistent with a previous report (4). The centrosomal staining of TAP68 was identical to the TRF1 labeling, which becomes apparent when the two images are merged (Fig. 3B, panel l, arrowhead). The centrosomal localization of TAP68 in nocodazole-treated cells indicated that its localization at the centrosome was independent of microtubules (Fig. 3D). These results indicate that TAP68 and TRF1 form a complex at the telomeres in interphase, and they migrate to centrosomes during mitosis.
The apparent temporal order of assembly of TAP68 and TRF1 to the centrosomes in mitosis led us to hypothesize that TAP68 may be important for TRF1 centrosomal localization. To test the role of TAP68 in TRF1 targeting, the localization of different segments of TAP68 tagged with GFP was examined.
Western blot analysis showed that all the constructs, including the full-length GFP-TAP68, expressed proteins of the predicted size (Fig. 3E). To verify that the GFP tag would not interfere with localization of TAP68, the full-length TAP68 tagged with GFP was first tested for its targeting. In metaphase cells, GFP-TAP68 localized to centrosomes where the majority of TRF1 was also observed (Fig. 3F, panels a, b, and d, arrows). Thus, the GFP tag did not alter exogenous TAP68 localization. We then assessed the centrosomal localization of TAP68 deletion mutants relative to TRF1 in transfected HeLa cells. A schematic representation of TAP68 deletion mutants was shown in Fig.  3G. All TAP68 mutants containing the first coiled-coil domain (amino acids 243-475) were clearly localized to the spindle poles (Fig. 3F, panel f). In contrast, the C terminus of TAP68 containing the second coiled-coil domain and TRF1-binding region (amino acids 332-593) failed to localize to the spindle poles (Fig. 3G). Thus, the centrosomal localization domain of TAP68 resided within the first coiled-coil domain (Fig. 3F, panels f and j, arrows), which is sufficient for its centrosomal local-FIGURE 1. Identification of a novel TRF1-binding protein, TAP68. A, mitotic HeLa cell extracts were applied to a TRF1 peptide antibody affinity column. After binding, columns were extensively washed, and bound proteins were eluted and separated by SDS-PAGE. The indicated proteins were extracted from the gel and digested with trypsin, and the amino acid sequence of the peptides was determined by MALDI-TOF and LC-MS/MS mass spectrometry. P1 is TAP68. B, protein band of P1 was extracted from the acrylamide gel and digested with trypsin, and the resulting peptide fragments were subjected to mass spectrometric analysis. C, P1 peptides match a previously uncharacterized protein with a 593-amino acid open reading frame of unknown function (AAH03618). D, schematic representation of the TAP68 domain organization predicted by NCBI's on-line conserved domain search tool. E, TRF1-TAP68 interaction was confirmed by immunoprecipitation assay. The anti-TRF1 antibody binding on protein A/G beads was incubated with mitotic cell lysate prepared in high stringency lysis buffer containing 1% Nonidet P-40 and 0.5% deoxycholate. Under this condition, TAP68 remained firmly bound to TRF1, whereas the majority of other TRF1-binding proteins, such as TRF2 and tankyrase, were absent from the co-immunoprecipitates. MAY 16, 2014 • VOLUME 289 • NUMBER 20

JOURNAL OF BIOLOGICAL CHEMISTRY 14149
ization. However, the centrosomal localization of TRF1 was lost with the TAP68MC mutant, suggesting that TAP68 was required for TRF1 recruitment to the centrosome in mitosis (Fig. 3F, bottom panel).
TAP68 Specifies the Centrosomal Localization of TRF1 during Mitosis-Targeted deletion of murine TRF1 resulted in an embryonic growth defect that was independent of telomere length regulations (37). Additional evidence has implicated TRF1 in the regulation of the mitotic spindle by interacting with a microtubule plus end-binding protein (EB1) (17). If TAP68 was required for the localization of TRF1 to the centrosome, depletion of TAP68 would abolish the centrosomal localization of TRF1. To examine the functional role of endogenous TAP68 in TRF1 localization to the centrosome, synchronized HeLa cells were depleted of TAP68 by transfection with small interfering RNA (siRNA) duplexes specific for TAP68. Western blot analysis confirmed the efficiency of TAP68 knockdown by its specific siRNA duplex but not by a scrambled siRNA. In addition, TAP68 siRNA does not alter TRF1, tankyrase 1, and NuMA protein levels (Fig. 4A). The observed 8-fold inhibition of TAP68 pro-tein expression represented a more than 90% depletion of TAP68 in 91 Ϯ 2% of the successfully transfected cells.
Next, we examined the requirement of TAP68 for targeting TRF1 to the centrosome. In TAP68-depleted cells, the centrosomal localization of TRF1 was reduced; however, no changes in NuMA association with the centrosome were noted (Fig. 4B), indicating the dependence of TRF1 on TAP68 to locate to the centrosome. Notably, depletion of TAP68 resulted in multiple spindle poles judged by the NuMA labeling (Fig. 4B, panel e) and ␥-tubulin staining (Fig. 4B, panel i), suggesting that TAP68 functions in bipolar spindle formation and/or centrosomal stability. Further examination of tankyrase 1, a TRF1-binding protein, in TAP68-depleted cells revealed that very little tankyrase 1 was associated with the centrosomes with most remaining associated with chromosomes. Thus, stable localization of TRF1 and tankyrase 1 to the centrosome was dependent on TAP68.
The average proportion of mitotic cells in bipolar, tripolar, and tetrapolar spindles in HeLa cells treated with TAP68 siRNA oligonucleotides was 14.3, 56.0, and 29.7%, respectively (Fig. 4C).  ). B, exogenous TAP68 interacts with TRF1 in HeLa cells transfected with FLAG-tagged TRF1 and either GFP or GFP-tagged TAP68. After 36 h, HeLa cells were then extracted with 1% Nonidet P-40 plus 0.5% deoxycholate, and GFP-tagged TAP68 protein and its associated proteins were precipitated with 20 l of GFP antibody-conjugated beads. Co-precipitated proteins were then detected by immunoblot for the presence of GFP-TAP68 (upper panel) or FLAG-TRF1 proteins (lower panel). C, schematic representation of different TAP68 truncation constructs. D, HeLa cells were transfected with full-length or different truncations of GFP-tagged TAP68 along with FLAG-tagged TRF1. After 36 h, HeLa cells were then extracted with 1% Nonidet P-40 plus 0.5% deoxycholate, and FLAG-tagged TRF1 and its accessory proteins were precipitated with 20 l of rabbit TRF1 antibody-conjugated beads. Co-precipitated proteins were then immunoblotted for the presence of GFP-TAP68 proteins (upper panel) or FLAG-TRF1 (lower panel).
In contrast, the average percentages of mitotic cells in bipolar, tripolar, and tetrapolar spindles in HeLa cells treated with control siRNA oligonucleotides were 96.0, 6.0, and 2.0%, respectively. Thus, the proportion of mitotic cells with bipolar spindles was significantly decreased in TAP68 siRNA-transfected cells (p Ͻ 0.001). In contrast, tripolar and tetrapolar spindles were significantly increased in TAP68 siRNA-expressing cells (both p Ͻ 0.001; Fig. 4C).
TAP68 Residues Thr-221 and Thr-457 Are Phosphorylated by NEK2A and PLK1 during Mitosis-We next tested whether the changes in TAP68 localization during mitosis were induced by its phosphorylation. TAP68 was analyzed in lysates of HeLa cell that were synchronized at G 1 , S, G 2 , and M phases. In mitotic cells, a slower mobility TAP68 protein band was apparent compared with that of interphase cells (Fig. 5A). -Phosphatase treatment of the mitotic lysate induced a shift in TAP68 to the faster migrating form, although treatment of interphase cells with the serine/threonine phosphatase inhibitor, okadaic acid, resulted in a shift to the slower migrating form (Fig. 5B). These findings provide evidence that TAP68 is phosphorylated during mitosis.
To define the region and specific residues of TAP68, which are phosphorylated during mitosis, we used anti-TAP68 anti-bodies to immunoprecipitate the TAP68 protein from mitotic HeLa cell lysates. Mass spectrometric analysis of the TAP68 protein isolated from mitotic cells suggested that both Thr-221 and Thr-457 were phosphorylated (data not shown). Computational analysis predicted that Thr-221 was a substrate of NEK2A, and Thr-457 was a substrate of PLK1 (38,39). To test whether Thr-221 was a substrate of NEK2A, we performed in FIGURE 3. TAP68 mediates TRF1 localization to the centrosome. A, TAP68 and TRF1 share similar subcellular localization. Nuclear and post-nuclear fractions were generated from interphase HeLa cell homogenates. Equal amounts of proteins (35 g) from various fractions were separated by 5-15% gradient SDS-PAGE and detected by Western blot analyses using antibodies against TAP68, TRF1, and ␤-tubulin, respectively. B, representative images of interphase (top panels), late prophase/early prometaphase (middle panels), and metaphase (bottom panels) HeLa cells stained for TRF1 (green), TAP68 (red), DAPI (blue), and ␥-tubulin (white). In interphase cells, TRF1 (panel a) and TAP68 (panel b) are readily seen in the nucleus as speckles and dots in the merge panel (panel d). In late prophase or the earliest prometaphase cells marked by nuclear membrane fragmentation, TAP68 appears at the centrosome (panel f, arrows), and TRF1 remains as speckles in the partially condensed chromosome (panel e, arrows). Both TAP68 and TRF1 become co-localized to the centrosome as the chromosomes align or after sister chromatid separation (panels i and j, arrows). A merged image shows the co-localization of TRF1 and TAP68 to the centrosome of mitotic cells (panel l). Bars, 10 m. C, immunoelectron microscopy of late prophase cells indicated that TAP68 localizes to the pericentrioles. D, representative immunofluorescence images of HeLa cells treated with nocodazole or DMSO. 10 h after drug treatment, the cells were fixed and stained for tubulin (green), TAP68 (red), and DAPI (blue). TAP68 remained centrosome-associated in the absence of microtubules (panel h, arrows). E, ectopic expression of different GFP-tagged TAP68 proteins. HeLa cells were transfected GFP-tagged full-length TAP68 or its deletion mutants. After 36 h, HeLa cells were then harvested for Western blot analysis using a GFP antibody. F, representative images collected from metaphase HeLa cells transiently transfected with GFP-tagged TAP68 and its deletion mutants and stained for TRF1 (red), TAP68 (green), and DAPI (blue). In cells expressing full-length TAP68, both TAP68 and TRF1 are co-localized to the centrosome (panels a, b, and d, arrows). A similar co-distribution of TRF1 with TAP68 was observed in TAP68⌬C mutant-expressing cells (panels e, f, and h) but not TAP68MC-expressing cells (panels i, j, and l), demonstrating that the centrosomal localization region of TAP68 is independent of its TRF1-binding domain. Bars, 10 m. G, schematic representation of the regions of TAP68, which specifies its centrosomal localization. H, representative images of HeLa cells transfected with different GFP-tagged TAP68 constructs. 24 h after transfection, cells were fix and co-stained for ␥-tubulin (red) and DNA (blue). Bars, 10 m.  (panels a-c). In TAP68 siRNA duplex-treated cells, TAP68 protein expression was suppressed (panels f and j), and localization of TRF1 to the centrosome was diminished, although TRF1 distribution on chromosome remained (panel g). The distribution of tankyrase 1 to the centrosome was also eliminated in TAP68-depleted cells (panel k). However, the spindle pole localization of NuMA was not altered by the loss of TAP68 (panel e). Tetrapolar spindle in TAP68-depleted cells was revealed by NuMA staining (panel e). Bars, 10 m. C, quantitative analyses of the mitotic spindle after TAP68 knockdown. HeLa cells were transfected with TAP68 siRNA oligonucleotide and control oligonucleotide for 36 -48 h followed by fixation and staining for DNA, TAP68, and NuMA. After examination under a fluorescence microscope, the centrosome instability phenotypes were categorized as bipolar, tripolar, or tetrapolar spindles. Data represent the percentage of total mitotic cells evaluated. An average of 100 cells from three separate experiments was counted. * indicates a significant difference between the TAP68 siRNA-and control siRNA-transfected cells.
vitro phosphorylation on recombinant histidine-tagged fusion proteins, including both wild-type TAP68 and two mutant TAP68 proteins in which threonine 221 and 457 were replaced by alanine (T221A and T457A, respectively). The histidinetagged wild-type and mutant TAP68 (T221A and T457A) migrated at about 70 kDa as shown in Fig. 5C. Incubation of the fusion proteins with [ 32 P]ATP and the recombinant NEK2A kinase resulted in the incorporation of 32 P into the TAP68-WT and TAP68-T457A proteins but not TAP68-T221A mutant (Fig. 5C). NEK2A-mediated phosphorylation was specific, because incubation of TAP68 with [ 32 P]ATP in the absence of the kinase resulted in no detectable incorporation of radioactivity into the wild-type protein (Fig. 5C, 1st lane). Next, we performed in vitro kinase assay using TAP68-WT and TAP68 -FIGURE 5. Phospho-regulation of TRF1-TAP68 interaction and centrosomal localization during mitosis. A, immunoblot analysis of TAP68 in HeLa cells synchronized at the indicated cell cycle phases. Note the mobility shift (pTAP68) from mitotic cell lysates. B, HeLa cell lysates (35 g) from synchronized interphase and mitotic cells were treated with -phosphatase or the phosphatase inhibitor, okadaic acid, followed by Western blotting with an anti-TAP68 antibody. C, bacterially expressed histidine-tagged TAP68 fusion proteins, both wild-type and mutant (TAP68 T221A and TAP68 T457A ), were phosphorylated in vitro in the presence of [ 32 P]ATP and NEK2A kinase. Samples were separated by 6 -16% gradient SDS-PAGE. The gel was dried and subsequently incubated with x-ray film. Note that in the presence of NEK2A, there was a dramatic incorporation of 32 P into the wild-type TAP68 and TAP68 T457A mutant, but not the TAP68 T221A mutant. D, purified His-tagged TAP68-WT and TAP68-T457A were subjected to in vitro phosphorylation by recombinant PLK1 kinase. The left panel shows Coomassie Blue staining of the gel. The right panel shows the result of autoradiography. E, HeLa cell lysates from synchronized G 1 and nocodazoletreated mitotic cells ectopically expressing wild-type or the indicated FLAG-TAP68 mutants were immunoblotted with an anti-FLAG and anti-␣-tubulin antibodies, respectively. F, lysates from mitotic HeLa cells ectopically expressing FLAG-tagged TAP68-WT and the indicated mutants, immunoprecipitated with an anti-TRF1 antibody, and then immunoblotted with anti-TRF1 and anti-FLAG antibodies. TAP68 T221A , which cannot be phosphorylated by NEK2A, was co-precipitated with TRF1 (lane 4), and its phospho-mimicking mutant TAP68 T221E did not interact with TRF1 (lane 6). In contrast, the mutant with mimics Thr-457 phosphorylation by PLK1, TAP68 T457E , interacted with TRF1, whereas the TAP68 T457A mutant did not (lanes 10 and 8, respectively). Thus, phosphorylation of TAP68 at Thr-221 by NEK2A releases it from TRF1, whereas phosphorylation at Thr-457 by PLK1 is required for the TRF1-TAP68 interaction. G, representative images of HeLa cells ectopically expressing wild-type and mutant FLAG-TAP68. Cells were fixed and stained for TAP68 (red), TRF1 (green), and DNA (blue). Bars, 10 m. Both wild-type FLAG-TAP68 and TRF1 are co-localized to the centrosome at metaphase (panels a and b, arrows). A merged image shows the co-localization of TRF1 and wild-type FLAG-TAP68 to the centrosome of mitotic cells (panel d). However, in HeLa cells ectopically expressing TAP68 T221A , which cannot be phosphorylated by NEK2A, TAP68 T221A remains associated with TRF1 at the telomere in late prometaphase cells (panel f) as evident in the merged image (panel h). In HeLa cells ectopically expressing TAP68 T457A , which cannot be phosphorylated by PLK1, TAP68 T457A localizes to the centrosome (panel j) with little TRF1 (panel i) as evidenced in the merged image (panel l, arrows), illustrating that PLK1-mediated phosphorylation is essential for TAP68-TRF1 association at the centrosome. Indeed, TRF1 co-localizes with TAP68 in HeLa cells ectopically expressing the phospho-mimicking mutant, TAP68 T457E .
457A as substrates, respectively. Recombinant PLK1 kinase phosphorylated TAP68-WT strongly. In contrast, its phosphorylation toward TAP68 -457A is very weak (Fig. 5D). Together, these studies confirmed the mass spectrometric analysis, and indicated that Thr-221 of TAP68 was a substrate of NEK2A, whereas Thr-457 was a substrate of PLK1. Mutation of Thr-221 and Thr-457 to alanine partially reduced FLAG-TAP68 phosphorylation in mitotic cells as determined by the mobility shift that was abolished with -phosphatase treatment (Fig. 5E), suggesting that NEK2A and PLK1 act cooperatively to phosphorylate TAP68 during mitosis. Thus, we concluded that both Thr-221 and Thr-457 are phosphorylated during mitosis.
Phosphorylation of TAP68 at Thr-457 Is Essential for the Association of TRF1 with TAP68 at the Centrosomes-TAP68 translocated to the spindle poles at the onset of prophase or early prometaphase (Fig. 3B), and this correlated with its phosphorylation (Fig. 5A), suggesting that phosphorylation of TAP68 may induce its dissociation from the telomere at the G 2 /M boundary. To evaluate the phospho-regulation of TAP68-TRF1 interaction, we used anti-TRF1 antibodies to immunoprecipitate TRF1, and bound TAP68 proteins from lysates of mitotic arrested HeLa cells exogenously express FLAG-tagged wild-type and mutant TAP68. Western blot analysis of the immunoprecipitate with anti-FLAG antibodies showed that both wild-type and the TAP68-T221A co-precipitate with TRF1 (Fig. 5F, 2nd and 4th lanes), although this association was abolished with the phosphorylation-mimicking mutant, TAP68-T221E (Fig. 5F, 8th lane). Interestingly, phosphorylation of Thr-457 was essential for TAP68-TRF1 interaction as TAP68-T457E was bound to TRF1 (Fig. 5F, 10th lane). Dephosphorylation of Thr-457 by phosphatase abolished the association of TAP68 with TRF1 (Fig. 5F, 6th lane), confirming the importance of Thr-457 in mediating TRF1-TAP68 interaction.
To correlate TAP68 phosphorylation with its centrosomal localization, both wild-type FLAG-TAP68 and phosphorylation mutant constructs were transiently transfected in HeLa cells. Both wild-type and the PLK-dependent phospho-mimicking mutant TAP68-T457E were localized to the centrosome (Fig. 5G, panels b and n, arrows) with a co-distribution of TRF1, indicating that phosphorylation of TAP68 at Thr-457 by PLK1 is essential for its association with TRF1 and for targeting TRF1 to the centrosome. Indeed, inhibition of Thr-457 phosphorylation (TAP68-T457A) prevented the localization of TRF1 to the centrosome (Fig. 5G, panel i, arrows). In contrast, the NEK2Adependent phosphorylation-deficient mutant, TAP68-T221A, remained associated with TRF1 at the telomeres as the cells progressed toward metaphase (Fig. 5G, panels e-h), indicating that phosphorylation at Thr-221 was required for its dissociation from telomeric TRF at prophase or early prometaphase. This was consistent with the fact that TRF1 failed to precipitate TAP68-T221A from mitotic cell lysate. Thus, we concluded that NEK2A and PLK1 orchestrate TRF1-TAP68 interaction during mitosis.

DISCUSSION
Telomeres are essential for genome stability in all eukaryotes; dysfunctions in their assembly are associated with the chromo-somal instability characteristic of cancer and aging (1,3). Human telomere function requires two telomere-specific DNA TTAGGG repeat-binding proteins, TRF1 and TRF2, which interact with an array of proteins, including tankyrase, TIN2, PINX1, and protection of telomeres 1 (POT1) (1,3). TRF1 and its binding proteins, such as tankyrase, relocate to the centrosome at the onset of mitosis (4 -6). However, the molecular mechanisms underlying telomere dynamics and function in mitosis are less understood. In this study, we identified a novel centrosomal structural maintenance of chromosome domain protein, TAP68, which interacts with TRF1 and specifies its centrosomal localization in mitotic cells, providing insight into the function of the TRF1 protein complex in mitotic regulation. Immunoelectron microscopy confirmed that TAP68 localizes to the pericentrioles of mitotic cells. Moreover, depletion of TAP68 was sufficient to prevent TRF1 centrosomal translocalization and induced genomic instability. Interestingly, TAP68 is phosphorylated by NEK2A and PLK1, which regulate its transit from the telomere to the centrosome. Collectively, our results support a model in which TAP68 provides a functional link between telomeres and centrosomes in human cells. It would be of great interest to establish the structure-function relationship of TAP68 using structure-based analyses (40), which will enable us to consolidate the phospho-regulation into dynamic spindle regulation.
Oscillations in mitotic kinase activity drive progression through mitosis. A key mitotic kinase, NEK2A, exhibits a dynamic pattern of distribution during mitosis, which is important for faithful chromosome segregation during mitosis (36,(41)(42)(43)(44). NEK2A is associated with the centrosome in interphase cells and becomes associated with spindle pole upon the breakdown of the nuclear envelope. Our finding that NEK2A phosphorylates TAP68 at Thr-221 in early prometaphase is consistent with the spatiotemporal dynamics of NEK2A during mitosis (36). In fact, TRF1/Pin2 was identified previously as both a protein (TRF1) that binds to telomeric DNA repeats (45) and as a protein (Pin2) that associates with the NIMA kinase (46), a NEK2A homologue in Aspergillus nidulans essential for entry into mitosis. We envision a model in which TAP68 is phosphorylated by NEK2A at the telomere at early prometaphase, which releases TAP68 for its trafficking to the centrosome where it is then phosphorylated by PLK1. Further studies will employ phospho-amino acid-specific antibodies to elucidate the spatiotemporal profile of phospho-TAP68 during mitosis. Given its close association with centrosome apparatus, it would be also important to ascertain if TAP68 exhibits microtubule binding activity and whether its association with microtubule is regulated by PLK1/Nek2A (47).
Abnormal centrosomal structure is frequently observed in cancer tissues and may be responsible for promoting disease progression (29,48). As shown in this study, depletion of TAP68 in HeLa cells produced chromosome instability phenotypes similar to those seen in fission yeast in which aberrant expression of exogenous Pcp1 caused mitotic spindle defects, chromosome segregation abnormality, and supernumerary centrosomal structures (49). In fission yeast, disruption of TAZ1-Pcp1 interaction resulted in an anaphase bridge phenotype due mainly to the inability to resolve end-to-end chromo-some fusions. In human cells, sister chromatid separation at the centromere and arms, but not at the telomeres, was associated with suppression of tankyrase 1 (50). In addition, a role for tankyrase 1 in bipolar spindle assembly has been reported (25,26). Thus, conserved centrosome-telomere linkage events may play a role in mitotic chromosome segregation. Further studies will evaluate TAP68 levels in cancer cells with known chromosome instability to assess its possible role in disease progression.
Tankyrase is a poly(ADP-ribose) polymerase that transfers ADP-ribose to glutamic acid residues of its substrates (1). The localization of poly(ADP-ribose) to the centrosome is very similar to that of tankyrase (5). Perturbation of poly(ADP-ribose) by inhibitory antibody neutralization or poly(ADP-ribose) glycohydrolase treatment results in multipolar spindle assembly (26), which is similar to that seen in TAP68-depleted cells (Fig.  4B). The loss of tankyrase 1 at centrosomes correlated with the spindle multipolarity observed in TAP68-deficient cells, suggesting that poly(ADP-ribosylation) of centrosomal spindle proteins may be required for bipolar spindle formation and function in mitosis, consistent with Chang et al. (26).
NuMA is a spindle protein required for spindle pole assembly; it interacts directly with tankyrase 1 (51,52). However, PARsylation of NuMA is not required for spindle function, which is consistent with our observation that centrosomal localization of NuMA is independent of tankyrase 1 (52). It would be interesting to elucidate the function and regulation of tankyrase 1 at the centrosome as well as its contribution to mitotic stability by identifying its substrates at the spindle pole. Proteomics-based strategies, such as those used in this study, that employ a combination of affinity isolation and mass spectrometry with biochemical manipulation of tankyrase 1 level at the spindle pole will allow us to unravel the molecular components whose functions are regulated by tankyrase 1-mediated protein PARsylation and mechanisms underlying normal centrosomal and telomeric function during mitosis.
This study is limited in that the function and clinical relevance of TAP68 remain unknown. Further studies will evaluate the effects of TAP68 silencing on cell proliferation as greater cell proliferation was observed with TRF1 lox/lox mice (16). TAP68 suppression may also influence cell cycle progression. Knockdown of the TRF1-interacting protein, PinX1, delayed entry into mitosis, due to the loss of stabilization of TRF1 by PinX1 (53). Therefore, further studies will analyze the effects of TAP68 knockdown and TAP68 mutant expression on cell cycle progression. Alternatively, TAP68 may have a role in telomere maintenance in a similar fashion to Pin1 (54), which will be assessed in future studies.
In summary, we describe a new TRF1-interacting protein, TAP68, which localizes to telomeres in interphase, migrating to centrosomes following nuclear envelope breakdown. At the centrosomes, TAP68 recruits TRF1. Repression of TAP68 results in genomic instability, which may be mediated by inhibition of TRF1 and tankyrase recruitment to centrosomes.