Genetic Analysis of the Structure and Function of 7SK Small Nuclear Ribonucleoprotein (snRNP) in Cells*

Background: HEXIM1 and LaRP7 bind to 7SK snRNA. Results: HEXIM1 and LaRP7 activation domain chimeras activated plasmid targets via defined 7SK snRNA motifs in cells. Conclusion: Specific RNA targets of HEXIM1 and LaRP7 and inhibition of P-TEFb were dissected genetically in vivo. Significance: This system facilitates studies of 7SK snRNP in cells. The positive transcription elongation factor b (P-TEFb), comprised of cyclin-dependent kinase 9 (CDK9) and cyclins T1 (CycT1) or T2 (CycT2), activates eukaryotic transcription elongation. In growing cells, P-TEFb exists in active and inactive forms. In the latter, it is incorporated into the 7SK small nuclear ribonucleoprotein, which contains hexamethylene bisacetamide-induced proteins (HEXIM) 1 or 2, La-related protein 7 (LaRP7), methyl phosphate capping enzyme, and 7SK small nuclear RNA (7SK). HEXIM1 inhibits the kinase activity of CDK9 via interactions between 7SK, HEXIM1, and CycT1. LaRP7 and methyl phosphate capping enzyme interact with 7SK independently of HEXIM1 and P-TEFb. To analyze genetic interactions between HEXIM1 and/or LaRP7 and 7SK using a cell-based system, we established artificial heterologous RNA tethering assays in which reporter gene expression depended on interactions between selected regions of 7SK and its cognate binding partners fused to a strong activator. This system enabled us to map the HEXIM1- and LaRP7- binding regions of 7SK. Assays with various mutant 7SK plasmid targets revealed that the 5′ U-U bulge and central loop of stem-loop I or RNA motif 3 of 7SK are required for transactivation, suggesting that HEXIM1 and CycT1 form a combinatorial binding surface for 7SK. Moreover, a region in HEXIM1 C-terminal to its previously mapped RNA-binding motif was also required for interactions between HEXIM1 and 7SK. Finally, a tyrosine-to-alanine mutation in HEXIM1, which is critical for its inhibitory effect on CDK9, changed HEXIM1 into an activator. These cell-based assays elucidate this important aspect of transcription elongation in vivo.

self-inhibitory domain (27). The central basic domain (residues 150 -177) forms a nuclear localization signal and an RNA-binding domain (27). Interestingly, the sequence of the HEXIM1 nuclear localization signal is almost identical to the argininerich motif of Tat (26). In vitro studies indicated that HEXIM1 binds to double-stranded RNA in a relatively sequence-independent manner (28). Indeed, the same study demonstrated that HEXIM1 also binds to microRNAs (miR16) (28). Immediately C-terminal to this region (residues 178 -220) are found acidic amino acids, the PYNT motif (residues 203-206) and the evolutionally conserved phenylalanine at position 208 (Phe-208), which interact with CycT1 and help to inhibit the kinase activity of CDK9 (26,29,30). This acidic region also binds to the basic region of HEXIM1 in the absence of 7SK and, in its presence, improves these RNA-binding interactions (31). Finally, the C-terminal coiled-coil domain (residues 250 -359) interacts with CycT1 and supports the formation of HEXIM1 dimers (29,32,33). This region also contains an additional tyrosine residue at position 271 (Tyr-271) that is critical for the inhibition of CDK9 (30).
In this study, we established an experimental approach to analyze genetic interactions between HEXIM1, LaRP7, and 7SK using a cell-based system. We adopted an artificial heterologous RNA tethering reporter assay (34 -37) and constructed new plasmid targets by replacing the TAR of HIVSCAT with various regions of 7SK. By recruiting P-TEFb via hybrid proteins that also contained the transcriptional activation domain of Tat (residues 1-48), the HEXIM1⅐Tat48 and LaRP7⅐Tat48 chimeras stimulated plasmid targets that contained M3 and M8, respectively. The central region of HEXIM1 (residues 150 -220) was sufficient to bind to M3. Deletion of U40/U41 or disruption of the GAUC motif abolished this activation, supporting previous binding studies in vitro (38). In addition, the central loop (U57/58) of M3 was also required for transactivation, suggesting that HEXIM1 and CycT1 form a combinatorial binding surface for 7SK, which resembles interactions between Tat, CycT1, and TAR. Finally, mutating Tyr-271 of HEXIM1 to alanine turned HEXIM1 into an activator on M3. Therefore, we established a reliable cell-based assay to analyze genetic interactions between HEXIM1, 7SK, and P-TEFb.

EXPERIMENTAL PROCEDURES
Cell Lines and Antibodies-HeLa or HEK293T cells were grown in DMEM containing 10% FCS at 37°C. Rabbit anti-cMyc (catalog no. ab9106) and anti-␤ tubulin (catalog no. ab6046) antibodies were purchased from Abcam. Rabbit anti-CDK9 (catalog no. SC-484) was purchased from Santa Cruz Biotechnology.
Plasmids-Chloramphenicol acetyltransferase (CAT) plasmid targets were constructed by inserting double-stranded DNA into pHIVSCAT using BglII and SacI restriction enzymes (34,38). The cDNAs of the RNA sequences from 7SK snRNA and their negative strand DNAs were chemically synthesized (IDTDNA) and annealed to form double-strand DNA inserts. Both the 5Ј and 3Ј ends of each cDNA and the negative strand contain unique linkers to create the adhesive ends of the restriction enzymes and to maintain the Watson-Crick base pairing that forms an RNA stem structure as in the TAR region of the original HIVSCAT. The linker sequences were as follows: cDNA strand, 5Ј-AGATCT (cDNA) GAGCT-3Ј; negative strand, 5Ј-C (negative strand DNA) A-3Ј. The actual DNA sequences of each reporter are available upon request. Fulllength or truncated HEXIM1 and the HEXIM1 chimera were constructed in the pEF-Bos mammalian expression plasmid. All proteins contained a cMyc antibody epitope tag at the N terminus, and their expression in cells was confirmed by Western blot analysis using an anti-cMyc antibody. The CDK9⅐ CycT1 chimera was created as described previously, except that the full-length CycT1 was fused to CDK9 (39). As described previously, the CDK9⅐CycT1 chimera forms an active P-TEFb complex when expressed ectopically in cells (39). Point mutations were created by using the QuikChange site-directed mutagenesis kit (Agilent Technologies).
RNA Immunoprecipitation-HEXIM1 (Figs. 3B and 4) or Hex⅐Tat48 fusion proteins (Fig. 4) were expressed in HEK293T cells (4 ϫ 10 6 ) by transient transfection using Lipofectamine 2000 (Invitrogen) in the presence (Fig. 3B) or absence (Fig. 4) of the WT or mutant M3 plasmid reporters (0.5 g). The cells were lysed in buffer A (20 mM HEPES-KOH (pH 7.8), 0.1 M KCl, 0.1% Nonidet P-40, and 0.2 mM EDTA) on ice for 10 min. Cell lysates were centrifuged at 14,000 rpm for 5 min at 4°C, and supernatants were collected. Cell lysates were then precleared with protein A-Sepharose beads (Invitrogen) and divided into two aliquots. Each aliquot was incubated with 1 g of normalrabbit IgG or anti-cMyc antibodies overnight at 4°C. Then, 20 l of protein-A-Sepharose beads precoated with BSA and yeast tRNA were added to each aliquot for an additional 2 h at 4°C. Beads were washed five times with buffer A. RNA was then extracted with TRIzol (Invitrogen), followed by DNase I treatment (Ambion). Reverse transcription quantitative PCR (RT-qPCR) analyses were performed to quantify endogenous 7SK ( Fig. 4) or RNA expressed from the plasmid reporters ( Fig. 3B) enriched in the immunoprecipitates. The same sets of qPCR analyses using the samples without reverse transcription confirmed that the DNA contamination from transfected plasmid reporters was negligible (data not shown). The specific primers for endogenous 7SK or RNA from the reporter plasmids were as follows: 7SK, GAGGGCGATCTGGCTGCGACAT (forward) and ACATGGAGCGGTGAGGGAGGAA (reverse); 7SK-CAT, TTTCCCAGGAAGA TCCTGAC (forward) and ACCT-TGGTGAGATCGAATGG (reverse). Data were normalized to input amounts of 7SK and calculated as values relative to the amount obtained with IgG control of untransfected cells (set to 1).
Transient Transfection and CAT Assay-HeLa cells were seeded into 24-well plates approximately 12 h prior to cotransfection of the plasmid reporters (0.1 g) and plasmids encoding the effectors (0.3 g for Hex and Hex chimeras or 0.1 g for LaRP7 and the LaRP7 chimera) using Xtreme HP (Roche). Forty-eight hours after transfection, cells were lysed in and subjected to CAT enzymatic assays as described previously (36). The remaining lysates were subjected to Western blot analysis using anti-cMyc antibody and anti-tubulin antibody as a loading control.

HEXIM1 and LaRP7
Bind to M3 and M8, Respectively, in Cells-Wassarman and Steitz (40) originally predicted the secondary structure of 7SK. Their model contains four RNA stemloops (Fig. 1A, SLI-SLIV). Recently, Bensaude and colleagues (20) published a modified structure that contains eight evolutionally conserved motifs (Fig. 1B, M1-M8) . In vitro binding studies indicated that sequences from positions 24 -87 of 7SK, which correspond to M3 or short SLI, interact with HEXIM1. SLIV/M8 interacts with LaRP7 (24,(41)(42)(43). To study genetic interactions between HEXIM1 or LaRP7 and 7SK, we modified an artificial heterologous RNA tethering system (34). In these systems, the TAR in the HIV LTR is replaced by an RNA sequence of interest (34), and RNA-binding proteins that interact with it are used as fusion partners of specific effectors. One well studied plasmid target contains the stem-loop IIB (SLIIB) from the HIV Rev response element and Rev proteins fused to activators of transcription elongation (35,37,44). In particular, CycT1 from P-TEFb is a strong activator when fused to Rev, as are the transcriptional activation domain of Tat (residues 1-48, TAD48) and RelA, a subunit of nuclear factor B (44, 45).
We first created chimeras between HEXIM1 or LaRP7 and Tat48 (Fig. 2). Because Tat48 binds to CycT1 and not to RNA (46), our chimeras could only activate these plasmid targets when their fusion partners bound to their cognate RNA sequences. Indeed, Tat48 did not activate plasmid reporters used in this study (see below). Next, we constructed plasmid targets with nucleotides forming the proposed secondary structures, which are presented in Fig. 1A. They are short SLI/M3, SLII, SLIII/M7, and SLIV/M8. HEXIM1 (Hex), Hex⅐Tat48, LaRP7, or LaRP7⅐Tat48 chimeras were coexpressed with these plasmid targets by transient transfection in HeLa cells. Fortyeight hours after the transfection, CAT enzymatic activities were measured and calculated as fold activation over the values obtained with non-fused proteins. As presented in Fig. 2, the Hex⅐Tat48 chimera activated M3 7-fold over Hex alone (Fig. 2, columns 1 and 2) but not other plasmid targets (Fig. 2, columns 6, 10, and 14). On the other hand, the LaRP7⅐Tat48 chimera activated M8 5-fold over LaRP7 alone (Fig. 2, columns 15 and 16). It also failed to activate other plasmid targets (Fig. 2, columns 4, 8, and 12). Western blotting of cell lysates revealed amounts of ectopically expressed proteins. We conclude that HEXIM1 and LaRP7 bind to M3 and M8, respectively.

Analysis of 7SK snRNP Formation by Reporter Assays
An extensive in vitro study conducted by Dock-Bregeon and co-workers (43) indicated that the U-U bulge (U40/U41) as well as the GAUC:CUAG stem adjacent to the U-U bulge (positions 42-45 and 64 -67, respectively) are critical for interactions between HEXIM1 and 7SK. To examine the importance of these motifs in our CAT reporter system, we created mutant M3 plasmid targets in which the U-U bulge was removed (M3⌬40/41) or the GUAC motif was mutated (M3A43G/ U44C) (Fig. 3A). Surprisingly, although Tat and the Hex(150 -177)⅐Tat48 chimera retained a lower but significant activity on these mutant plasmid targets (Fig. 3A, columns 6, 8, 10, and 12), the Hex(150 -220)⅐Tat48 chimera failed completely to activate them (Fig. 3A, columns 7 and 11). The full-length Hex⅐Tat48 chimera also failed to activate these plasmid targets (data not shown). To determine whether RNAs expressed from the WT or mutant M3 plasmid targets interact with HEXIM1, we performed additional RNA immunoprecipitations. HEXIM1 was coexpressed with WT and mutant plasmid targets, followed by immunoprecipitations with anti-cMyc antibodies. RNAs associated with HEXIM1 were extracted, treated with DNase I, and quantified by RT-qPCR using primer sets specific to the 3Ј region of M3 and the 5Ј proximal region of the CAT gene. A parallel qPCR analysis of the same RNA samples without RT reaction confirmed that the RT-qPCR signals were not from the plasmid DNA (data not shown). As presented in Fig. 3B, although the WT M3 RNA was enriched (ϳ100-fold over the IgG control) in HEXIM1 immunoprecipitations, it was not observed with mutant M3⌬40/41 and M3A43G/U44C RNAs (Fig. 3B, columns 2-4). From these results, we conclude that HEXIM1 interacts specifically with M3 and that residues from positions 178 -220 of HEXIM1 are critical for interactions between HEXIM1 and 7SK.
U Residues in the Central Loop of M3 Are Also Important for Interactions with HEXIM1-In vitro binding studies indicated additional sequences in M3 that might be involved in interactions between HEXIM1 and 7SK snRNP. For instance, deletion of the uridine at position 63 or the C-U bulge at positions 71 and 72 reduce this binding affinity (43). In addition, there is another bulge (from positions 75-77) containing a uridine residue that could interact with HEXIM1. Finally, 7SK contains a central loop (from positions 50 -59) that resembles the central loop of TAR where CycT1 binds to form a stable complex between Tat, P-TEFb, and TAR (21,47,48). Therefore, we constructed a series of mutant M3 plasmid targets to identify other residues critical for these interactions. Next, we determined whether the Hex(150 -220)⅐Tat48 chimera can activate these plasmid targets (Fig. 5). As presented in Fig. 5, the mutant M3⌬U63, M3⌬C34/C35, M3⌬C71/U72, and M3⌬C75/U77 plasmid targets were activated by the Hex(150 -220)⅐Tat48 chimera to levels similar to the WT M3 plasmid target, indicating that they still interact with HEXIM1 in cells (Fig. 5, bars 1 and 6 -9). In contrast, the Hex(150 -220)⅐Tat48 chimera activated the mutant ⌬U57/U58 plasmid target to a lesser extent (Fig. 5, bars 4 and 5). This finding and the previous data on residues from positions 178 -220 of HEXIM1, which contribute to its binding specificity (Fig. 3), support the model where RNA-binding residues from positions 150 -177 and those from positions 178 -220 of HEXIM1 form a tripartite HEXIM1-CycT1-7SK complex that resembles the Tat-CycT1-TAR complex (21,47,48).
Mutating the Tyrosine at Position 271 to Alanine Transforms HEXIM1 into an Activator-Previous results indicated that HEXIM1 activates M3 only when the Hex⅐Tat48 chimera recruits P-TEFb. Because Tat can compete with HEXIM1 for binding to CycT1 (22,23), P-TEFb recruited via Tat48 is also not inhibited by HEXIM1 in the context of the Hex⅐Tat48 chimera. However, HEXIM1 alone inhibited M3 (Fig. 7, bar 1), indicating that interactions between HEXIM1 and M3 are sufficient to inactivate P-TEFb. Mutating a tyrosine at position 271 to alanine (Y271A) abolishes this inhibitory effect of HEXIM1 without affecting its ability to interact with 7SK and CycT1 (30). Indeed, the mutant Hex(Y271A) proteins exhibited a positive effect (ϳ4-fold) on reporter M3 (Fig. 7, bar 3). The mutant Hex(Y271A) proteins did not activate other plasmid targets, such as HIVSCAT (data not shown). Therefore, HEXIM1 can activate plasmid targets via 7SK when its ability to inhibit P-TEFb is diminished.

Recruitment of Active (Free) P-TEFb Is Required for Optimal Activation by the Mutant Hex(Y271A) Protein-Although a
Tyr-to-Ala mutation at position 271 changed HEXIM1 into an activator (Fig. 7), its effects on M3 were small (4-fold, Fig. 7). Because the majority of P-TEFb is found in the inactive 7SK snRNP in HeLa cells and because ectopically expressed  HEXIM1(178 -220) determines the target specificity of HEXIM1. A, the RNA sequence and putative secondary structure of plasmid effectors are presented above the CAT data. 7SK plasmid targets (indicated in each panel of CAT data) were coexpressed with effectors, which are presented below the CAT data, in HeLa cells. CAT data are presented as fold activation by calculating relative CAT values obtained with Tat48 fusion proteins over those obtained with the empty plasmid (columns 1, 5, and 9). Error bars are as in Fig. 2. The expression of effectors was determined by Western blotting with anti-cMyc antibodies with tubulin as the loading control (bottom panel). B, HEXIM1 interacts with the WT M3 but not the mutant M3⌬U40/41 or M3A43G/C44U plasmid targets. 7SK plasmid targets (indicated below the graph) were coexpressed with HEXIM1 in HeLa cells. HEXIM1 (Hex, cMyc epitope-tagged) was immunoprecipitated by anti-cMyc antibodies, and associated RNA was subjected to RT-qPCR using primer sets specific to these plasmid targets. Data are presented as fold RNA enrichment by calculating relative amounts of cDNA over the value obtained with non-transfected cells (column 1 set at 1). Immunoprecipitations (IP) with IgG from cells expressing HEXIM1 and each plasmid target were performed as negative controls (C) and confirmed that the signals are specific to HEXIM1 immunoprecipitations (data not shown).
HEXIM1 proteins are inefficiently incorporated into the 7SK snRNP (Ref. 17 and data not shown), the lower activation by the mutant Hex(Y271A) protein could be due to limited amounts of active P-TEFb in cells. To examine this possibility, we constructed the Hex⅐CycT1 chimera that contained WT HEXIM1 or mutant Hex(Y271A) proteins and determined its ability to activate M3 (Fig. 8A). Although the WT Hex⅐CycT1 chimera weakly activated this plasmid target (1.5-fold), the mutant Hex(Y271A)⅐CycT1 chimera exhibited a strong effect (12-fold, Fig. 8A, columns 2 and 3). The Hex⅐CycT1 and the mutant Hex(Y271A)⅐CycT1 chimeras were expressed at similar levels (Fig. 8A). Similarly, the mutant Hex(Y271A) protein activated M3 when the active P-TEFb complex (CDK9⅐CycT1 chimera) was provided in trans by transient coexpression (8-fold, Fig. 8B,  columns 2 and 4). We conclude that the optimal activation of M3 by the mutant Hex(Y271A) protein requires the active P-TEFb in cis or in trans.

DISCUSSION
In this study, we established a reporter-based system to measure genetic interactions between 7SK and HEXIM1 or LaRP7. In agreement with previous studies, HEXIM1 bound to M3, and LaRP7 bound to M8. The former interaction was mediated not only by the U-U bulge and GUAC motif of 7SK but also U residues in its central loop. Finally, a mutation at Tyr-271 to Ala transformed HEXIM1 into an activator.
In our heterologous RNA tethering system to study interactions between 7SK and HEXIM1, Tat48 was used as the effec-tor. Maximal activities obtained with the Hex⅐Tat48 or LaRP7⅐Tat48 chimeras were somewhat lower (5-to 8-fold) than those obtained with other systems, such as Rev and SLIIB (20to 30-fold) (34,35,37,44). This difference could reflect the ability of Rev to form higher-order oligomers that recruit more activators to the elongation complex. Although HEXIM1 forms dimers on 7SK (32,49), only one HEXIM1-binding site was identified in our study. Also, because of high levels of the endogenous HEXIM1 protein, it was not clear whether our Hex⅐Tat48 chimera bound to the reporter as a monomer or a dimer with itself or the endogenous HEXIM1 protein. Finally, WT 7SK contains seven AUG codons that reduced the efficiency CAT protein expression using the endogenous in-frame start codon. However, RT-qPCR data supported our CAT data, i.e. that transcription of the longer reporter genes that contained the full-length 7SK were activated 3-to 5-fold by the Hex⅐Tat48 chimera (data not shown).
In the absence of Tat, RNAPII transcribes up to 150 nucleotides past the transcriptional start site on the HIV LTR (50). Supporting this observation, recent genome-wide analyses revealed that pausing sites distribute over an extended distance after initiation (51). These findings suggest that stable RNA structures are only surrogate markers for pausing rather than stalling RNAPII by themselves. Therefore, transcription from our 7SK plasmid targets should exhibit patterns of RNAPII pausing similar to those on the parental HIV LTR. To this end, when TAR was substituted with the stemloop IIB (SL((B) from the Rev response element, the Rev⅐CycT1 chimera activated HIV transcription indistinguishably from Tat and TAR (35).
HEXIM1 can be recruited to promoter and 5Ј proximal regions in genes directly or via RNA-binding proteins such as SC35, presumably for loading of P-TEFb (52,53). Although chromatin immunoprecipitations revealed HEXIM1 and LaRP7 on DNA, they did not demonstrate the presence of 7SK or the entire 7SK snRNP. Of interest, we also observed tight associations between HEXIM1 and chromatin but only when it was phosphorylated on serine at position 158 (54). Thus modified, the phosphorylated HEXIM1 protein neither bound 7SK nor inhibited P-TEFb (54). Therefore, it would be of interests to determine whether the chromatin-bound HEXIM1 is capable of interacting with 7SK. Nevertheless, we observed effects of HEXIM1 only in the presence of M3 and not without its specific RNA target.
Our results indicated that the minimal HEXIM1-binding region of 7SK contains the U-U bulge (positions 40 and 41), the GUAC motif, and the central loop. No other predicted secondary structures were found to bind to HEXIM1. This region also binds to Tat, which is consistent with previous studies in vitro (21). Although those binding studies demonstrated the importance of the U40-U41 bulge and the GUAC motif for the interaction between HEXIM1 and 7SK (43), the importance of the central loop for assembling the 7SK snRNP had not been appreciated. In contrast, our results revealed that the uridine residues at positions 57 and 58 in the central loop are also required. It is to be noted that, in addition to the basic region of HEXIM1 (from positions 150 -177), its immediate C-terminal residues (from positions 178 -220) support specific interactions To quantify 7SK, RNA in immunoprecipitations was subjected to RT-qPCR by using a set of specific primers. Data are presented as fold RNA enrichment by calculating relative amounts of cDNA over values obtained with untransfected cells (column 1 set at 1). Immunoprecipitation with IgG was performed as a negative control (C) and confirmed that the signals were specific to HEXIM1 immunoprecipitations (data not shown). Note that Hex(150 -220)⅐Tat48 and Hex(178 -220)⅐Tat48 chimeras (118 and 70 residues, respectively) were not retained quantitatively on nitrocellulose filters because of their small sizes.
between HEXIM1 and 7SK (26). This region contains the PYNT motif (from positions 203-206) and the phenylalanine at position 208, which also help to inhibit the kinase activity of CDK9 (26,29,30). These RNA-protein interactions cause a conformational change to create a combinatorial binding surface for CycT1, which resembles those between Tat, TAR, and CycT1 (23,31) where the central loop of TAR is required for binding to CycT1 (46). Our results provide a genetic and func-  Reporters are presented next to the CAT data. The Hex(150 -220)⅐Tat48 chimera (0.3 g) was coexpressed with each reporter (0.1 g) in HeLa cells. CAT data are presented as fold activation by calculating relative CAT values over those obtained with empty plasmids. Error bars are as in Fig. 2. m, cMyc epitope tag; lsSLI, lower stem of SLI; sM3, short M3. pA, polyadenylation site. tional support for this model (Fig. 9). Because cooperative interactions between HEXIM1 and P-TEFb require the proper spatial arrangement between the 5Ј U-U bulge and the central loop in M3, which is not maintained in TAR, HEXIM(Y271A) did not work on TAR. On the other hand, the LaRP7-binding region of 7SK was mapped to M8 (Fig. 2).
The maintenance of the P-TEFb equilibrium is a critical step in transcription elongation. At homeostasis, the amount of active P-TEFb is limited because it is mostly sequestered in the 7SK snRNP. Therefore, the release of P-TEFb from 7SK snRNP by various stimuli or stresses is a prerequisite step for activation of transcription and for cellular growth. However, Tat can recruit P-TEFb directly from the 7SK snRNP (22,23). Not only does Tat contain an RNA-binding region almost identical to that in HEXIM1, but TAR folds into a stem-loop similar to the minimal HEXIM1-binding region of 7SK. As such, Tat can compete with HEXIM1 and 7SK for binding to CycT1, which forms a stable tripartite complex with TAR (22). Therefore, it is intriguing to speculate that HIV evolved by modifying HEXIM1 and removing its P-TEFb inhibitory sequences and adjusting its RNA-protein interaction to obtain the simplest unit for transcription elongation of HIV.
In conclusion, our reporter-based assays provide a quantitative measurement of RNA-protein interactions that assemble 7SK snRNP. They facilitate the analysis of these interactions, which could be used to measure efficiencies of compounds that interfere with and to develop high throughput screening systems for inhibitors that block these RNA-protein interactions in cells.