Casein Kinase 1 α Phosphorylates the Wnt Regulator Jade-1 and Modulates Its Activity*

Background: Jade-1 localizes to the primary cilium and centrosome and inhibits canonical Wnt signaling. Results: Casein kinase 1 α phosphorylates Jade-1 at a conserved SLS motif. Conclusion: Jade-1 phosphorylation abrogates its ability to inhibit β-catenin signaling. Significance: These results contribute to understanding β-catenin regulation, which is central to discovering new therapeutic targets for diseases involving the Wnt signaling pathway. Tight regulation of Wnt/β-catenin signaling is critical for vertebrate development and tissue maintenance, and deregulation can lead to a host of disease phenotypes, including developmental disorders and cancer. Proteins associated with primary cilia and centrosomes have been demonstrated to negatively regulate canonical Wnt signaling in interphase cells. The plant homeodomain zinc finger protein Jade-1 can act as an E3 ubiquitin ligase-targeting β-catenin for proteasomal degradation and concentrates at the centrosome and ciliary basal body in addition to the nucleus in interphase cells. We demonstrate that the destruction complex component casein kinase 1α (CK1α) phosphorylates Jade-1 at a conserved SLS motif and reduces the ability of Jade-1 to inhibit β-catenin signaling. Consistently, Jade-1 lacking the SLS motif is more effective than wild-type Jade-1 in reducing β-catenin-induced secondary axis formation in Xenopus laevis embryos in vivo. Interestingly, CK1α also phosphorylates β-catenin and the destruction complex component adenomatous polyposis coli at a similar SLS motif to the effect that β-catenin is targeted for degradation. The opposing effect of Jade-1 phosphorylation by CK1α suggests a novel example of the dual functions of CK1α activity to either oppose or promote canonical Wnt signaling in a context-dependent manner.

Tight regulation of Wnt/␤-catenin signaling is critical for vertebrate development and tissue maintenance, and deregulation can lead to a host of disease phenotypes, including developmental disorders and cancer. Proteins associated with primary cilia and centrosomes have been demonstrated to negatively regulate canonical Wnt signaling in interphase cells. The plant homeodomain zinc finger protein Jade-1 can act as an E3 ubiquitin ligase-targeting ␤-catenin for proteasomal degradation and concentrates at the centrosome and ciliary basal body in addition to the nucleus in interphase cells. We demonstrate that the destruction complex component casein kinase 1␣ (CK1␣) phosphorylates Jade-1 at a conserved SLS motif and reduces the ability of Jade-1 to inhibit ␤-catenin signaling. Consistently, Jade-1 lacking the SLS motif is more effective than wild-type Jade-1 in reducing ␤-catenin-induced secondary axis formation in Xenopus laevis embryos in vivo. Interestingly, CK1␣ also phosphorylates ␤-catenin and the destruction complex component adenomatous polyposis coli at a similar SLS motif to the effect that ␤-catenin is targeted for degradation. The opposing effect of Jade-1 phosphorylation by CK1␣ suggests a novel example of the dual functions of CK1␣ activity to either oppose or promote canonical Wnt signaling in a context-dependent manner.
Wnt/␤-catenin signaling has long been recognized as a critical regulator of vertebrate development (1,2). More recently, the role of Wnt/␤-catenin signaling in pluripotent stem cell renewal and adult tissue repair has been increasingly explored (3)(4)(5). In differentiated cells, ␤-catenin functions as an adhesion molecule, and unbound cytosolic ␤-catenin is degraded rapidly. However, up-regulated Wnt signaling prevents degradation and allows unbound ␤-catenin to translocate to the nucleus, where it acts as a transcription factor for several genes involved in proliferation (for a review, see Refs. 2, 6, 7). ␤-catenin signaling during repair is critical in multiple adult organ systems, including the kidney (8,9), colon (10,11), lung (12), liver (13), bone (14), and central nervous system (15,16). Consistently, deregulated Wnt/␤-catenin signaling is associated with diseases ranging from Alzheimer disease and schizophrenia to osteoporosis and cystic kidney disease (9,15,17). Furthermore, ␤-catenin accumulation is widely associated with tumorigenesis, and several tumor types have underlying germ line or sporadic mutations that disrupt ␤-catenin degradation (18,19). The context-dependent mechanisms regulating ␤-catenin signaling continue to be discovered, but it remains difficult to selectively and temporally target specific components of this pathway.
Several reports have demonstrated that the primary cilium and centrosome restrain ␤-catenin signaling in interphase cells (20 -22), and that several components of the ␤-catenin destruction complex localize to the base of the primary cilium (23)(24)(25). Recently, our group demonstrated that Jade-1, an E3-ubiquitin ligase that targets ␤-catenin for proteasomal degradation (26), localizes to the primary cilium and is regulated by the ciliary nephrocystin protein complex (27). Specifically, nephrocystin-4 (NPHP4) stabilizes a non-phosphorylated form of Jade-1, increasing Jade-1 nuclear translocation and inhibiting ␤-catenin signaling. Loss of nephrocystin complex function leads to nephronophthisis, the leading genetic cause of endstage kidney failure in children. To further determine the mechanisms underlying Jade-1 regulation of ␤-catenin signaling, we sought to identify specific kinases responsible for the phosphorylation of Jade-1. We now report that the classical Wnt pathway component casein kinase 1 ␣ (CK1␣) specifically phosphorylates Jade-1 at an unprimed SLS phosphorylation site, initiating a phosphorylation cascade that abrogates the ability of Jade-1 to inhibit ␤-catenin signaling.
Cell Culture-HEK 293T cells were cultured in DMEM (Sigma) supplemented with 10% FBS as described previously (27). For transfection experiments, cells were seeded in 10-cm or 6-well dishes, grown to 60% confluency, and transiently transfected with plasmid DNA using the calcium phosphate method as described previously (31,32). For luciferase and siRNA experiments, cells were grown to 80% confluency in 6-well or 96-well plates and transfected with Lipofectamine 2000 (Invitrogen) according to the instructions of the manufacturer. 24 h post-transfection, cells in 6-well dishes were harvested in 1 ml of cold PBS, and the centrifuged pellet was boiled in Laemmli buffer at 95°C for 10 min to obtain whole cell lysate. Cells in 10-cm dishes were harvested with 6 ml of cold PBS and centrifuged, and the cell pellet was used for cell fractionation or coimmunoprecipitation experiments as described previously (27). Proteins were immunoprecipitated using anti-FLAG-agarose beads (M2, Sigma), nickel-nitrilotriacetic acid beads (Qia-gen), or 2 g of antibody coupled to protein G beads (GE Healthcare), as indicated in the figure legends. For cell fraction experiments, all nuclear pellets were resuspended in a high-salt buffer, sonicated, and boiled in Laemmli buffer. Densitometry values were obtained using Image Studio Lite version 4.0.21 (LI-COR Biosciences) and calculated as follows. Each cytosolic F.␤-catenin value was normalized by the respective cytosolic ␤-tubulin value in each condition. Nuclear F.␤-catenin values were likewise normalized by nuclear fibrillarin. Values for each protein analyzed were set to a percentage across each image to control for exposure times. Experiments were normalized by setting total ␤-catenin values to 1 across all conditions independently for the cytosol and nucleus. Differences were assessed by one-way ANOVA.
Ubiquitinated Protein Detection-Transfected HEK 293T cells were incubated with MG132 (1 M for 7 h) prior to harvesting 48 h post-transfection. Ubiquitinated proteins were detected according to a modified protocol from Choo et al. (33). Cell monolayers were washed with PBS and scraped in 150 l of lysis buffer (2% SDS, 150 mM NaCl, and 10 mM Tris-HCl (pH 8.0)) with 2 mM Na 3 VO 4 , 50 mM PMSF, and 25 mM N-ethylmaleimide and boiled immediately at 95°C for 10 min. Boiled lysates were sonicated and diluted in 900 l of dilution buffer (10 mM Tris-HCl (pH 8.0), 150 mM NaCl, 2 mM EDTA, and 1% Triton) prior to incubation with rotation at 4°C for 60 min. Lysates were subsequently centrifuged for 30 min at 20,000 ϫ g, and supernatants were equalized for protein concentration according to the BCA protein assay (Thermo Scientific). 30 l of equalized protein lysate was immediately boiled with Laemmli buffer as an "IP lysate." 950 l of equalized protein lysate was incubated overnight with 30 l of FLAG (M2) beads. After incubation, beads were washed three time in wash buffer (10 mM Tris-HCl (pH 8.0), 1 M NaCl, 1 mM EDTA, and 1% Nonidet P-40) and boiled with Laemmli buffer for 5 min at 95°C, and then binding proteins were resolved by SDS-PAGE. Ubiquitinated F.␤-catenin values were determined using densitometry software (Image Studio Lite version 4.0.21, LI-COR Biosciences) and calculated as follows. The densitometry value for the HA-ubiquitin smear corresponding to immunoprecipitated ␤-catenin was normalized by the heavy chain of the precipitated beads, and this value was then divided by the densitometry value for FLAG staining, representing the immunoprecipitated F.␤-catenin normalized by the heavy chain of the precipitated beads. To control for variation in exposure intensity, the sum of measured values in an individual blot was set to 100%. Differences were assessed by one-way ANOVA.
Reagents-MG132 InSolution was purchased from Calbiochem. LiCl was purchased from Merck and resuspended in water. D4476 was purchased from Sigma and resuspended in dimethyl sulfoxide. Cells previously transfected for 24 h were incubated in a final concentration of 100 M D4476 for an additional 24-h period. D4476 was transfected using a modified protocol to enhance solubility (34) whereby 10 l of a 10 mM stock solution was diluted in 50 l of Opti-MEM (Invitrogen) and incubated with 3 l of Lipofectamine 2000 (Invitrogen) for 20 min at room temperature before application to cells in 1 ml of DMEM. Control cells were incubated with an equal amount of dimethyl sulfoxide. BI 2536 was purchased from Selleck Chem-icals and resuspended in water. Cells previously transfected for 24 h were incubated in a final concentration of 100 nM BI 2536 for an additional 24-h period.
In Vitro Kinase Assay and Direct Interaction-For downstream coprecipitation and mass spectrometry applications, 500 ng of GST-tagged CK1␣ (Sigma, catalog no. SRP5013) was incubated for 15 min at 30°C in a 25-l reaction containing 10 l of the kinase assay buffer recommended by the manufacturer (diluted 5-fold in water plus 0.25 mM DTT) with or without 5 g of recombinant His-tagged Jade-1 truncation (amino acids 4 -174) and 80 nM (final concentration) ATP, as indicated in the figure legends. Recombinant His-tagged Jade-1 4 -174 was expressed according to the standard protocol in BL21 bacteria. Direct interaction was assessed by diluting the completed reaction with 1 ml of IP buffer and proceeding according to the immunoprecipitation protocol as described above.
Mass Spectrometry and Phosphoproteomic Analysis-Phosphoproteomic analysis of whole cell lysates was performed as described previously with slight modifications (35,36). Harvested HEK 293T cells were lysed in a buffer containing 8 M urea and 50 mM ammonium bicarbonate with added Pierce protease and phosphatase inhibitor mixture (Thermo Scientific). Protein concentration was determined by BCA protein assay (Thermo Scientific). 400 g of total protein was reduced using DTT and alkylated using iodoacetamide, as described previously, and peptides were digested using trypsin at a w/w ratio of 1:50 at 37°C for 16 h. After desalting procedure, phosphopeptides were enriched using ferric nitrolotriacetate immobilized metal affinity chromatography columns (Thermo Scientific). After final cleanup (ZipTip columns, Millipore), samples were analyzed using an LTQ Orbitrap Discovery mass spectrometer (Thermo Scientific) coupled to a Proxeon EASY-nLC II nano-LC system (Proxeon/Thermo Scientific). Intact peptides were detected at a resolution of 30,000 in the m/z range of 200 -2000 (MS). Internal calibration was performed using the ion signal of (Si(CH 3 ) 2 O) 6 H at m/z 445.120025 as a lock mass. For LC-MS/MS analysis, up to five collision-induced dissociation spectra (MS2) were acquired following each full MS scan. Sample aliquots were separated on a 15-cm, 75-m reverse phase column (Proxeon/Thermo Scientific). Gradient elution was performed from 10 -40% acetonitrile within 90 min at a flow rate of 250 nl/min. Generated raw data were searched using the SEQUEST algorithm against the most recent version of the Uniprot human database. The false discovery rate was adjusted to be lower than 0.01. Analysis and visualization of spectra were performed using the ProteomeDiscoverer software (Thermo Scientific). Label-free quantification and visualization of MS1 precursor masses were performed using the QUOIL software (37,38).
An in vitro kinase assay (described above) was used to detect specific residues phosphorylated by CK1␣. After reaction completion, peptides were reduced, alkylated, and digested using trypsin overnight, as described previously (35). Subsequently, peptides were subjected to C18 cleanup as described above and then resuspended in 10 l of 0.1% formic acid. Peptides were separated using a 90-min nLC-MS/MS gradient on a 15-cm C18 column (Dr. Maisch) and sprayed into a Q Exactive Plus mass spectrometer (Thermo, Bremen, Germany). Gradient set-tings are described above. The setting for peptide fragmentation on a Q Exactive plus mass spectrometer were as follows. An MS1 scan (resolution, 70,000; scan range, 300 -2000 m/z) was followed by 20 MS2 scans acquired in the Orbitrap (resolution, 75,000; isolation window, 3 m/z) using higher energy collisional dissociation fractionation. Dynamic exclusion was enabled (10 s).
Thermo raw files were analyzed using the MaxQuant software suite, as described previously, with the label-free quantitation option enabled (36,39,40). Briefly, carbamidomethylation of cysteine was put as a fixed modification, and phosphorylation and N-terminal acetylation was put as a variable modification. Peptide identifications were only accepted when they matched a protein and peptide identification false discovery rate of less than 0.01 and when the site localization false discovery rate was less than 0.01. Only high-confidence localized peptides (MaxQuant localization score Ͼ0.75) were used for further analysis. Spectra were displayed and annotated using MaxQuant viewer software (39).
Luciferase Assay-HEK 293T cells were seeded into 96-well plates and transfected as described above, with a total amount of 130 ng of DNA/well (50 ng of TOPFlash firefly luciferase, 25 ng of constitutively active Renilla luciferase pGL4.74, 5 ng of FLAG.␤-catenin, and 25 ng each of experimental plasmids as indicated in the figure legends; total DNA transfected was balanced when necessary using empty vector). Where indicated in the figure legends, 25 nM siRNA was used. Transfections, lyses, and measurements were performed as described previously (27). Significance was assessed for all ␤-catenin-stimulated treatment groups using a one-way analysis of variance with Tukey's post hoc test or two-way analysis of variance with post hoc Bonferroni-corrected paired Student's t tests. Error bars represent mean Ϯ S.E.
Xenopus laevis Microinjections and Double Axis Assay-X. laevis embryos were cultured, manipulated, and staged as described elsewhere (41). In brief, X. laevis eggs were obtained from females injected with 500 -800 units of human chorionic gonadotropin. Eggs were fertilized in vitro and dejellied with 3% cysteine. For microinjection, eggs were transferred into Ficoll (3% Ficoll in 0.3ϫ Marc's modified Ringer's medium). Microinjection of 10 nl containing the indicated amount of RNA into one ventral blastomere of Xenopus was performed at the fourcell stage. 12 h after injection, the embryos were transferred into 0.3ϫ Marc's modified Ringer's medium plus gentamicin and cultivated at 13°C until harvested and staged according to Nieuwkoop and Faber (63). Secondary axis formation was scored morphologically at stage 39. Statistical analysis was performed using Sigma-Stat 3.5 (Systat Software, Germany). All experiments were approved by the institutional animal committee (Regierungspräsidium Baden-Württemberg).
In Vitro Synthesis of mRNA-Plasmids were linearized with the following enzymes: Jade1 VF10, Sal1 and ␤-catenin CS2ϩMT, and NotI (5Ј deletion of 138 bp to eliminate a possible GSK3 sequence). Linearized plasmids were purified by phenol-chloroform. In vitro synthesis of synthetically capped mRNA was performed by in vitro transcription with T7 or SP6 polymerase using the mMessage mMachine kit (Ambion), and the RNA was purified using the RNAeasy mini kit (Qiagen).

Jade-1 Is Phosphorylated by CK1␣ at a Conserved SLS Motif-
To determine whether Jade-1 could be a substrate of a Wnt pathway kinase, we analyzed specific protein-protein interactions by coimmunoprecipitation experiments. V5-tagged short isoform Jade-1 (V5.Jade-1) was found to coprecipitate with two overexpressed classical Wnt/␤-catenin pathway kinases, FLAG-tagged GSK3␤ (F.GSK3␤) and FLAG-tagged CK1␣ (F.CK1␣). In these experiments, we observed that coexpression of F.CK1␣, but not F.GSK3␤, shifted the size of V5.Jade-1 to a higher molecular weight (Fig. 1A). With an endogenous coimmunoprecipitation experiment, we also demonstrated that both the long and short isoforms of endogenous Jade-1 coprecipitated with endogenous CK1␣ (Fig. 1B). Moreover, in vitro interaction experiments using recombinant purified proteins suggested that this interaction is direct (Fig. 1C). In our previous study, we described that Jade-1, in whole cell lysates, presents as a double band, and we proposed that the upper band might result from phosphorylation of Jade-1 (27). To confirm the size shift because of CK1␣ in nuclei-containing whole cell lysates, Western blot visualization of V5.Jade-1 coexpressed with empty vector was compared with that of V5.Jade-1 coexpressed with either F.CK1␣ or F.NPHP4. Consistent with our previous findings, coexpression with F.NPHP4 stabilized the smaller form of V5.Jade-1, whereas coexpression with F.CK1␣ stabilized the larger form of V5.Jade-1 (Fig. 1D). Conversely, inhibition of CK1␣ kinase activity using the specific CK1 inhibitor D4476 led to the stabilization of the smaller V5.Jade-1 expression size, whereas a control kinase inhibitor, BI 2536, had no effect (Fig. 1E). Strikingly, coexpression of a kinase-dead version of F.CK1␣ possessing a mutation of the lysine at residue 46 to aspartic acid (29) failed to stabilize the larger form of V5.Jade-1 and, instead, potently reduced expression size (Fig.  1F). Taken together, these data suggest that Jade-1 is phosphorylated by CK1␣.
To determine where a candidate phosphorylation site might be located, three truncations of V5.Jade-1 were expressed together with either F.CK1␣ or F.NPHP4 (Fig. 2, A and B). In both cases, expression of the first 150 amino acids was modified heavily, as is visible by the size shift, whereas expression of the C terminus was unchanged. The middle truncation appears to be only slightly modified by CK1␣. However it is strongly stabi- lized by NPHP4 coexpression. Sequence analysis of the Jade-1 N terminus identified several candidate CK1␣ phosphorylation motifs according to the consensus sequence of S/Tp-X-X-S/T (42,43). In addition, one candidate motif was identified at amino acid 18 that could be phosphorylated according to two possible unprimed consensus motifs: either because of the upstream cluster of acidic residues at amino acid positions 11-15 (44, 45) or because of the following two residues that, together, create an SLS phosphorylation motif. Such an unprimed SLS phosphorylation motif is also used by CK1␣ to phosphorylate the canonical Wnt pathway components ␤-catenin (46) and adenomatous polyposis coli (APC) (47), where unprimed phosphorylation by CK1␣ initiates a primed phosphorylation cascade in combination with GSK3␤ (Fig. 2C). This candidate multiple phosphorylation motif of human Jade-1 is highly conserved across species (Fig. 2D), as, indeed, is the entire protein sequence (data not shown). To determine whether a CK1-initiated phosphorylation cascade on the Jade-1 N terminus might require the tandem activity of GSK3␤, V5.Jade-1 was coexpressed with F.CK1␣ in the presence of LiCl, a known GSK3␤ inhibitor. LiCl treatment failed to interfere with the stabilization of the larger form of Jade-1 by F.CK1␣ (Fig. 2E), confirming CK1␣ as the major kinase for Jade-1, whereas GSK3␤ might have only a minor influence on the CK1␣-Jade-1 axis.
Phosphoproteomic analyses of HEK 293T cells expressing Jade-1 in the presence or absence of overexpressed CK1␣ were performed to confirm the phosphorylation of the Jade-1 residues Ser-18 and Ser-20. Although a dramatic increase in total Jade-1 phosphorylation in a peptide containing residues Ser-18 and Ser-20 could be shown in the presence of F.CK1␣ (Fig. 2F), initial attempts to localize the site specifically in the MS2 spectrum (CID) failed because of the multiple phosphorylatable residues in the N terminus. Therefore, a recombinant N-terminal peptide fragment of Jade-1 (amino acids 4 -174) was incubated in vitro with CK1␣ protein and ATP. MS/MS analysis using HCD fractionation (48) identified both single phosphorylated (Ser-18 and Ser-20 individually) as well as double phosphorylated (Ser-18 and Ser-20 together) peptides (Fig. 3, A and B). There was no evidence for phosphorylation of any of the other serine or threonine residues within this peptide sequence (on the basis of label-free quantification of MS1 precursor intensities (39,40)). In addition, there was no evidence for phosphorylation when ATP was omitted (Fig. 3B). To further confirm the requirement of residues Ser-18 and Ser-20 for CK1␣-mediated phosphorylation of Jade-1, a construct with point mutations of both residues was generated (V5.Jade-1 S18/20A). Mutation of both Ser-18 and Ser-20 to alanine abolished the expression of the larger form of V5.Jade-1 under basal conditions and significantly reduced the expression of the larger form induced by the presence of overexpressed F.CK1␣ but did not alter expression size in the presence of F.NPHP4 (Fig. 3, C and D). The single point mutation of serine 18 or double point mutation of the upstream acidic residues did not significantly alter Jade-1 expression (data not shown).
Jade-1 Phosphorylation Abrogates Its Ability to Inhibit ␤-Catenin Signaling-TOPflash TCF/LEF reporter assays were used to determine whether phosphorylation of Jade-1 altered the ability of Jade-1 to negatively regulate canonical Wnt signaling. Wild-type V5.Jade-1 and the S18A/S20A mutant version both reduced ␤-catenin-stimulated Wnt reporter activation. Overexpression of F.CK1␣ was not sufficient to activate TOPflash reporter activity but substantially potentiated ␤-catenin-stimulated reporter activity. In this condition, expression of V5.Jade-1 wild-type did not achieve a significant reduction of reporter activity, whereas V5.Jade-1 S18A/S20A lacking the intact bona fide CK1␣ phosphorylation motif did (Fig. 4A). Western blot analysis of whole cell protein expression in the luciferase transfection setting confirmed a stabilization of F.␤-catenin in the presence of F.CK1␣.
The influence of CK1␣ phosphorylation was further explored in vivo using the X. laevis model system. Overactive canonical Wnt signaling is well known to produce a double axis in developing X. laevis, and inhibition of this phenotype can be assessed by coinjecting mRNA into the developing embryo. Both wild-type and S18A/S20A mutant Jade-1 mRNA were able to reduce ␤-catenin-induced double axis formation (Fig.  4B). Consistent with our TCF/LEF reporter assay results, inhibition of double axis formation was enhanced significantly by the mutant S18A/S20A Jade-1 mRNA, demonstrating an in vivo phosphorylation-dependent difference in efficacy of ␤-catenin regulation by Jade-1. Subsequent studies were able to demonstrate a similar inhibition of CK1␣-induced double axis formation by both versions of Jade-1. However, at non-toxic doses of CK1␣, not a sufficient number of double axes was generated to determine a difference between the inhibition efficacies of wild-type and mutant Jade-1 (data not shown).
That CK1␣ acts in an inhibitory manner to reduce the ability of Jade-1 to inhibit Wnt signaling was further supported by RNAi knockdown of endogenous CK1␣. When endogenous CK1␣ protein levels were reduced by 50% using siRNA, the same amount of transfected V5.Jade-1 was significantly more FIGURE 2. The N terminus of Jade-1S is phosphorylated by CK1␣ on a conserved non-canonical SLS motif identical to that phosphorylated on APC and ␤-catenin by CK1␣. A and B, truncations of V5.Jade-1 were coexpressed in HEK 293T cells with a vector control and either F.CK1␣ (A) or F.NPHP4 (B) for 24 h and harvested as a whole cell lysate (WCL). In both cases, the N-terminal fragment containing the first 150 amino acids was modified heavily, whereas the C-terminal fragment was not affected. IB, immunoblot. C, the non-canonical unprimed SLS motif leads to phosphorylation of the initial serine (circled asterisk), which can prime sequential phosphorylation on multiple classical CK1␣ motifs (black asterisks). In two other canonical Wnt pathway proteins, ␤-catenin (46) and APC (47), the SLS motif initiates multiple sequential phosphorylation events by CK1␣ or GSK3␤ (red asterisks) that are important for destruction complex function. D, sequence alignment demonstrated 95% sequence homology between species for Jade-1, including the N-terminal CK1␣ phosphorylation motifs and the SLS motif at position 18 (only the N terminus shown). E, HEK 293T cells were transiently transfected with the indicated plasmids for 24 h prior to 24-h incubation with control (Co) medium or medium containing 40 M LiCl. After a total of 48 h post-transfection, cells were harvested as a whole cell lysate. Incubation with LiCl did not alter the ability of F.CK1␣ to stabilize the larger expression form of V5.Jade-1. F, HEK 293T cells were transiently transfected with V5.Jade-1 and either F.CK1␣ or an empty vector control for 48 h. Mass spectrometry analysis of whole cell lysates confirmed increased phosphorylation of an N-terminal fragment of V5.Jade-1 in the presence of overexpressed F.CK1␣ but was unable to determine specific phosphorylated residues because of the presence of multiple potential phosphorylation sites (lack of site-determining peak). Shown is a representative graph depicting the MS1 precursor ion chromatograms of the respective phosphorylated peptide. Three biological replicates were performed. effective in reducing TOPflash reporter activity (Fig. 4C). Consistently, coexpression of the F.CK1␣ K46D construct, which stabilizes the apparently non-phosphorylated smaller version of V5.Jade-1, augmented TOPflash reporter inhibition (Fig.  4D). It is notable that the kinase-dead version of F.CK1␣ failed to potentiate TOPflash reporter activity as the wild-type version does. We were unable to detect a significant difference in the effect of either wild-type or kinase-dead CK1 on TOPflash reporter activity in the presence of Jade-1 siRNA, indicating that Jade-1 does not influence CK1 in a reciprocal manner (data not shown).
Western blot visualization of overexpressed proteins in the luciferase transfection protocol did not demonstrate a large difference between total F.␤-catenin expression when coexpressed with wild-type versus mutant V5.Jade-1 and assessed as a whole cell lysate (Fig. 4A). However, an increased amount of ubiquitinated F.␤-catenin could be detected in the presence of mutant versus wild-type V5.Jade-1 (Fig. 4E). It is important to note that densitometry analysis of the HA-ubiquitin smear relative to the amount of F.␤-catenin precipitated did not reach statistical significance because of the variability between experiments, indicating that other factors likely play a role in modulating the ubiquitin ligase function of Jade-1. When F.␤-catenin expression was assessed specifically in the cytosol versus the nucleus, it appeared that coexpression of V5.Jade-1 S18/20A was better able than wild-type V5.Jade-1 to reduce expression of F.␤-catenin specifically in the nucleus (Fig. 4F). This effect persisted even in the presence of overexpressed F.CK1␣, although variability again indicates that other factors are at play. Of interest, the stabilization of F.␤-catenin in the presence of overexpressed F.CK1␣ appears to largely be seen in the cytosolic fraction.
The canonical Wnt signaling model predicts that CK1␣ should function within the destruction complex when there is no upstream Wnt receptor activation. Therefore, the augmentation of TCF/LEF reporter activity because of CK1␣ overexpression was unexpected. However, CK1␣ has also been implicated as a positive regulator of Wnt reporter activity in HEK293 cells (49) and is known to stabilize ␤-catenin and activate Wnt signaling in X. laevis embryos (50). Although it remains possible that CK1␣ acts as a positive regulator in our luciferase experiments, further examination of our system revealed that overexpressed F.␤-catenin substantially decreased expression of F.CK1␣ (Fig. 5, A and C), suggesting instead that the augmented signal could be due to factors that reduce stability of a negative regulator in the context of ␤-catenin expression. That this could be a specific effect is supported by our observation that overexpression of F.Dvl2, which also activates TOPflash reporter activity, does not decrease F.CK1␣ expression. Consistently, TOPflash reporter activity is only augmented by F.CK1␣ when the assay is activated by F.␤-catenin and not by F.Dvl2 (Fig. 5B). Further analysis revealed that, at a higher threshold of F.␤-catenin, V5.Jade-1 expression was also reduced (Fig. 5D), suggesting the possibility that a general downstream positive feedback exists when F.␤-catenin is stabilized in our cell-based system.

DISCUSSION
Regulation of Wnt/␤-catenin signaling is critical at all stages of mammalian life. In adults, injury repair requires activation of Wnt/␤-catenin signaling, but uncontrolled activation is associated with numerous diseases, including cancer. Specific, local, and temporal targeting of ␤-catenin remains difficult because of the numerous influencing factors modulating ␤-catenin signaling and an incomplete understanding of ␤-catenin functions. The protein Jade-1 is able to act as an E3 ubiquitin ligase for ␤-catenin in addition to the more classically recognized ␤-TrCP (26). However, little is known about the physiological situations that may dictate which ubiquitin ligase is required at which time and to what effect. We have demonstrated previously that a non-phosphorylated form of Jade-1 is stabilized by the cilium-and centrosome-associated protein NPHP4 and that this enhances the negative regulation of ␤-catenin signaling by Jade-1 (27). Here we demonstrate that the classical Wnt kinase CK1␣ phosphorylates Jade-1 and that this reduces the efficacy of Jade-1 to inhibit ␤-catenin signaling.
Collectively, our data support that the Jade-1 motif phosphorylated by CK1␣ is an N-terminal SLS site. By phosphoproteomic analysis using a highly accurate peptide fragmentation method, HCD (48), we show unambiguously that the Jade-1 N-terminal residues Ser-18 and Ser-20 are phosphorylated directly by CK1␣ in vitro. This phosphorylation site is likely to be a rate-limiting step in a sequential phosphorylation cascade because mutation of both serines to alanines significantly reduced the formation of the larger species of Jade-1 despite several primed S/Tp-X-X-S/T motifs remaining intact immediately following the mutation. Furthermore, a kinase-dead mutant version of F.CK1␣ failed to stabilize the larger expressed form of V5.Jade-1, indicating that CK1␣ is not merely acting as a facilitator for another kinase but is integral to initiating the massively phosphorylated N terminus seen in HEK 293T cell lysates in the presence of overexpressed F.CK1␣. Phosphorylation of the subsequent primed CK1␣ sites was not detected in the Jade-1 N-terminal fragment in our in vitro experimental setup, which indicates that another kinase may contribute to the sequential phosphorylation cascade in cells after initial priming by CK1␣. The kinase GSK3␤ acts in tandem with CK1␣ in the Wnt/␤-catenin signaling pathway. However, our data indicate that, despite the presence of the GSK3␤ consensus motifs within the Jade-1 N terminus, phosphoryla- CK1␣ Phosphorylates Jade-1 SEPTEMBER 19, 2014 • VOLUME 289 • NUMBER 38 tion initiated by CK1␣ in intact HEK 293T cells does not require GSK3␤ cooperation because incubation with LiCl failed to interfere with the F.CK1␣-induced shift of V5.Jade-1 expression size.
The Jade-1 unprimed SLS phosphorylation motif is highly conserved across species and is similar to that phosphorylated by CK1␣ on ␤-catenin (46) and APC (47). In both cell-based and X. laevis models of Wnt signaling, a mutant version of Jade-1 lacking this phosphorylation motif was more effective at inhibiting Wnt activity, and RNAi knockdown of endogenous CK1␣ enhanced the ability of Jade-1 to inhibit reporter activity. Collectively, these data suggest that CK1␣ could act to reduce the ability of Jade-1 to ubiquitinate ␤-catenin (Fig. 6). Indeed, the S18A/S20A mutant version of Jade-1 was better able to ubiquitinate overexpressed F.␤-catenin, although the variability of the results indicate that other factors are at play. As demonstrated by cell fractionation data, the stability of F.␤-catenin was differentially affected by F.CK1␣ in the nucleus versus the cytosol, suggesting that these factors may influence protein localization and substrate availability.
It is interesting to note that phosphorylation of the SLS motif on ␤-catenin has the opposite effect. Phosphorylation of Ser-45 on ␤-catenin negatively regulates canonical Wnt signaling by initiating a phosphorylation cascade that will contribute to cytosolic ␤-catenin degradation. These opposing roles of CK1␣ must depend on cellular events governing access of this kinase to each substrate. Individual components of the ␤-catenin destruction complex have been shown previously to act in opposing manners depending on the activation status of the Wnt signaling cascade (7,51). For example, phosphorylation of cytosolic ␤-catenin requires CK1␣ and GSK3␤ to work cooperatively within the destruction complex. However, when a Wnt ligand binds a Frizzled (Fz) receptor, the entire destruction complex is recruited to the plasma membrane, where CK1␣ and GSK3␤ phosphorylate lipoprotein receptor-related proteins 5 and 6 (LRP5/6) and enhance the LRP-mediated Wnt response (51)(52)(53). CK1␣ complexes with LRP5/6 at 30 min but not 1 h after Wnt stimulation (49). During this time, the adhesion junction proteins E-cadherin and p120-catenin are recruited to the LRP-Fz complex and initially facilitate Dishevelled (Dvl) phosphorylation. However, after 30 min, CK1␣ phosphorylates E-cadherin, which releases p120-catenin and prevents continued Dvl phosphorylation (49). These events mean that CK1␣, a kinase that, in interphase, constitutively acts to restrain inappropriate ␤-catenin activation, responds to a Wnt signaling event by temporally regulated opposing responses. Initially, CK1␣ contributes to signal propagation, but it subsequently disables a prolonged response.
A similar situation may govern whether CK1␣ phosphorylates Jade-1 or ␤-catenin. In interphase cells, phosphorylated ␤-catenin accumulates at the centrosome, whereas ␤-catenin can be observed within the ciliary lumen (20). In addition to accumulating in the nucleus, Jade-1 also accumulates at the centrosome and ciliary base (27), and its role in ubiquitinating this pool of ␤-catenin could be mediated by the centrosomal NPH proteins. CK1␣ accumulates at the centrosome when cells enter prophase (54), where it could either phosphorylate ␤-catenin to inhibit a Wnt response or phosphorylate Jade-1 to enhance it. The balance between these two possibilities might be tipped by NPHP4, which is shown by these data to modify the same N-terminal fragment of Jade-1, as does CK1␣ in an opposing manner. NPHP4 localizes to the centrosome in confluent cells (55) and might play a role when cells reach confluence in "turning off" a Wnt response. In subconfluent cells, NPHP4 is dispersed throughout the cytoplasm and could play an earlier role during this time in enhancing Jade-1 nuclear translocation. Of note, NPHP4 mRNA is up-regulated after injury (56). Because Jade-1 can also ubiquitinate non-phosphorylated ␤-catenin (26), the ability of cytosolic NPHP4 to enhance this might be particularly important during injury repair.
The relationship between CK1␣ and ␤-catenin is not fully understood. Our current data demonstrate that overexpressed CK1␣ stabilized F.␤-catenin and increased F.␤-catenin-induced TOPflash reporter activity. Without an LRP-mediated Wnt response, CK1␣ should theoretically act within the destruction complex as a negative regulator. However, reports of CK1␣ potentiating Wnt signaling have been described (49,50). Our current data suggests a novel route by which CK1␣ could potentiate a ␤-catenin signaling activity through phosphorylation of Jade-1. However, it is unlikely that the influence of CK1␣ on Jade-1 constitutes the major mechanism for the observed potentiation of TOPflash activity because RNAi knockdown of Jade-1 did not alter these results. Instead, it appears that the mechanism could involve phosphorylation of another unknown substrate because a kinase-dead CK1␣ inhibited TOPflash reporter activity. This mechanism may involve FIGURE 4. CK1␣ impairs the ability of Jade-1S to inhibit Wnt reporter activity, and this is dependent on an intact SLS motif. A, the TOPflash Wnt reporter plasmid was cotransfected with a constitutively active Renilla luciferase reporter plasmid plus F.␤-catenin and experimental plasmids as indicated. Background activation was determined by omitting experimental plasmids and was not included in the statistical analysis. All plasmids were transiently transfected for 24 h in HEK 293T cells, and relative light units (RLU) are shown after normalization to Renilla luciferase activity, setting activation because of F.␤-catenin alone to 100%. Coexpression of experimental plasmids significantly altered TOPflash reporter activity, as assessed by two-way ANOVA, with significant main effects across Jade-1 condition (F(2,32) ϭ 44.78, p Ͻ 0.0001) as well as the presence/absence of F.CK1␣. (F(1,32) ϭ 13; p ϭ 0.0024). Post hoc analyses determined that overexpressed wild-type and the S18A/S20A mutant of V5.Jade-1 both significantly reduced TOPflash activation stimulated by F.␤-catenin overexpression. Overexpressed F.CK1␣ potentiated TOPflash reporter activity. This potentiation was reduced significantly in the presence of the S18A/S20A mutant version of V5.Jade-1 but not by wild-type V5.Jade-1 (Bonferroni-corrected paired Student's t tests as post hoc with 15 possible comparisons. **, p Ͻ 0.001; ***, p Ͻ 0.0001. Western blot analysis of protein expression was obtained by scaling up transfection of the indicated plasmids (see "Experimental Procedures") and harvesting cells as a whole cell lysate (WCL) to visualize nuclear as well as cytosolic proteins. B, double axis assays in X. laevis were performed by mRNA injection into one of four blastomeres (ventral) at the indicated concentrations. Injection of ␤-catenin mRNA alone resulted in the formation of a secondary axis (white arrow). Injection of Jade-1 mRNA in combination with ␤-catenin mRNA significantly reduced secondary axis formation. The reduction of double axis formation was enhanced significantly when ␤-catenin mRNA was injected in combination with the Jade-1 S18A/S20A mutant mRNA. Results of three independent experiments are shown (n ϭ total injected embryos). Results were assessed by one-way ANOVA (F(3,11) ϭ 61.72, p Ͻ 0.0001) with Tukey's post hoc (*, p Ͻ 0.05; **, p Ͻ 0.01; ***, p Ͻ 0.001). uninj., uninjected. C and D, TOPflash luciferase experiments were performed in HEK 293T cells as described above with 24-h transient transfection of plasmids, reporter plasmids, and siRNA as indicated. Results were analyzed by one-way ANOVA (F(3,19) ϭ 1.584, p Ͻ 0.0001 (C); F(3,15) ϭ 4.977, p Ͻ 0.0001 (D)), and Tukey's post hoc analysis demonstrated that the ability of Jade-1 to inhibit F.␤-catenin reporter activity was enhanced significantly by either endogenous CK1␣ knockdown or coexpression of a kinase-dead version of F.CK1␣ (K46D). *, p Ͻ 0.05; **, p Ͻ 0.01; ***, p Ͻ 0.001. E, plasmids were transiently transfected as indicated in HEK 293T cells for 16 h prior to incubation with or without MG132 for 7 h at 1 M. Cells were harvested in SDS lysis buffer and processed according to the ubiquitin detection protocol (see "Experimental Procedures"). F.␤-catenin was immunoprecipitated, and, after boiling in Laemmli buffer, supernatants were divided in two and run on separate SDS gels to visualize both HA and FLAG staining. The non-phosphorylatable version of V5.Jade-1 S18A/S20A was more effective at ubiquitinating F.␤-catenin, although variability in densitometry analysis indicates that other factors may be involved. F, plasmids were transiently transfected as indicated in HEK 293T cells for 24 h prior to processing according to the cell fraction protocol (see "Experimental Procedures"). Overexpression of F.CK1␣ led to stabilized cytosolic (Cyt) F.␤-catenin. Overexpression of V5.Jade-1 S18A/S20A trended to reduced nuclear F.␤-catenin even when F.CK1␣ was coexpressed. The bar graphs represent densitometry values obtained from four independent experiments (see "Experimental Procedures"). Significant differences were found within the cytosolic data set as assessed by one-way ANOVA (F(3,15) ϭ 7.583, p ϭ 0.0078) with Tukey's post hoc (**, p Ͻ 0.01). Black circles are individual data points. CK1␣ Phosphorylates Jade-1 SEPTEMBER 19, 2014 • VOLUME 289 • NUMBER 38 positive feedback because of stabilization of ␤-catenin in an overexpression context. In this situation, F.␤-catenin substantially reduced the stabilization of F.CK1␣ protein. This effect was specific to ␤-catenin. Overexpressed Dvl2 neither decreased F.CK1␣ expression nor led to potentiated TOPflash reporter activity. Furthermore, overexpression of F.CK1␣ appeared to stabilize F.␤-catenin protein levels, again suggesting that an unknown factor may be acting on both proteins. At a higher threshold of F.␤-catenin expression, V5.Jade-1 was also destabilized, indicating that a positive feedback mechanism could exist in a more ubiquitous manner. Although it remains to be seen whether these results extend beyond our experimental context, it is interesting to consider that the difference in threshold levels of overexpressed ␤-catenin required to destabilize V5.Jade-1 versus F.CK1␣ could reflect the sensitivity of Wnt gene transcription to fold change rather than absolute ␤-catenin levels, which has been described as a mechanism to buffer response to the background "noise" of ␤-catenin fluctuation (57).
Threshold differences in ␤-catenin activity may furthermore contribute to achieving the temporal regulation necessary for operational transients of ␤-catenin activation to occur. In general, the last decade of research has produced numerous examples whereby the Wnt/␤-catenin cascade initiates its own down-regulation via genetic and protein regulation (49, 58 -62). Although the multiple mechanisms in place to restrain ␤-catenin signaling have been studied extensively, the mechanisms used to overcome these FIGURE 5. Overexpressed F.␤-catenin, but not F.Dvl2, decreases protein expression of F.CK1␣. A, plasmids were transiently transfected as indicated for 24 h in HEK 293T cells prior to harvesting as a whole cell lysate (WCL). Coexpression of F.␤-catenin, but not F.Dvl2, reduced expression of F.CK1␣. Asterisk, nonspecific; IB, immunoblot. B, TOPflash luciferase experiments were performed in HEK 293T cells as described above with 24-h transient transfection of reporter and experimental plasmids as indicated in separate experiments. Data were normalized to activation without F.CK1␣ in each situation. Results were analyzed by paired Student's t tests. F.CK1␣ significantly augmented TOPflash reporter activity because of F.␤-catenin expression (t(4) ϭ 3.879; *, p Ͻ 0.05) but did not alter reporter activity because of F.Dvl2 expression. RLU, relative light units. C, low or high amounts of overexpressed F.␤-catenin reduces the expression of F.CK1␣. HEK 293T cells were transiently transfected with plasmids as indicated and processed 24 h later as a whole cell lysate. D, whole cell lysates of HEK 293Ts cells transiently transfected for 24 h show reduced V5.Jade-1 expression in the presence of high but not low amounts of F.␤-catenin. restraints in differentiated cells are less well understood. Perhaps this pathway has evolved to use the same players in different contexts to ensure that negative regulators are "engaged otherwise" for a specific amount of time so that an effective signal can be achieved when required, such as during injury repair. This activation then triggers multiple system termination processes, a phenomenon that is telling in terms of how important it is that the pathway does not remain in a prolonged active state. Many of the nuances of Wnt/␤-catenin signaling remain to be discovered, and it will be exciting to determine the specific roles played, in particular, by ciliary and centrosomal proteins.