Glutamine Synthetase Stability and Subcellular Distribution in Astrocytes Are Regulated by γ-Aminobutyric Type B Receptors*

Background: GABAB receptors (GABABR) and glutamine synthetase (GS), are co-expressed in astrocytes. Results: GABABRs bind to, stabilize, and target GS to the plasma membrane. Conclusion: GABABRs are major determinants of GS subcellular targeting and stability. Significance: Astrocytic GABABRs may play an unexpected role in regulating neurotransmission by promoting GS stability. Emerging evidence suggests that functional γ-aminobutyric acid B receptors (GABABRs) are expressed by astrocytes within the mammalian brain. GABABRs are heterodimeric G-protein-coupled receptors that are composed of R1/R2 subunits. To date, they have been characterized in neurons as the principal mediators of sustained inhibitory signaling; however their roles in astrocytic physiology have been ill defined. Here we reveal that the cytoplasmic tail of the GABABR2 subunit binds directly to the astrocytic protein glutamine synthetase (GS) and that this interaction determines the subcellular localization of GS. We further demonstrate that the binding of GS to GABABR2 increases the steady state expression levels of GS in heterologous cells and in mouse primary astrocyte culture. Mechanistically this increased stability of GS in the presence of GABABR2 occurs via reduced proteasomal degradation. Collectively, our results suggest a novel role for GABABRs as regulators of GS stability. Given the critical role that GS plays in the glutamine-glutamate cycle, astrocytic GABABRs may play a critical role in supporting both inhibitory and excitatory neurotransmission.

Emerging evidence suggests that functional ␥-aminobutyric acid B receptors (GABA B Rs) are expressed by astrocytes within the mammalian brain. GABA B Rs are heterodimeric G-proteincoupled receptors that are composed of R1/R2 subunits. To date, they have been characterized in neurons as the principal mediators of sustained inhibitory signaling; however their roles in astrocytic physiology have been ill defined. Here we reveal that the cytoplasmic tail of the GABA B R2 subunit binds directly to the astrocytic protein glutamine synthetase (GS) and that this interaction determines the subcellular localization of GS. We further demonstrate that the binding of GS to GABA B R2 increases the steady state expression levels of GS in heterologous cells and in mouse primary astrocyte culture. Mechanistically this increased stability of GS in the presence of GABA B R2 occurs via reduced proteasomal degradation. Collectively, our results suggest a novel role for GABA B Rs as regulators of GS stability. Given the critical role that GS plays in the glutamineglutamate cycle, astrocytic GABA B Rs may play a critical role in supporting both inhibitory and excitatory neurotransmission.
␥-Aminobutyric acid (GABA) 3 is the major inhibitory neurotransmitter in the central nervous system (CNS). GABA B receptors (GABA B Rs) are metabotropic receptors that are widely expressed in the brain, which mediate the slow and prolonged inhibitory neurotransmission (1,2). Functional GABA B Rs are obligatory heterodimers and are composed of GABA B R1 (R1) and GABA B R2 (R2) subunits (3). They are localized both in pre-and post-synaptic sites where they respectively inhibit neurotransmitter release and activate potassium channels (2,4).
Glutamine synthetase (GS) is an essential enzyme that catalyzes the conversion of glutamate and ammonium ions to glutamine, and therefore plays a critical role in nitrogen detoxification (5). In the brain, GS expression is restricted to glial with high levels being evident in astrocytes (5,6,7,8).
GS plays an essential role in brain function. Disrupting GS expression specifically in astrocytes leads to neonatal death in mice. In humans deficits in GS activity are believed to contribute to numerous neuropsychiatric disorders, including temporal lobe epilepsy, Alzheimer disease, and schizophrenia (9, 10 -15, 16).
While the consequences of GS activity for regulating excitatory glutamatergic neurotransmission are self-evident, this enzyme also plays a critical role in supporting inhibitory neurotransmission since glutamine is the major metabolic precursor for neuronal GABA synthesis (17). Thus locally decreasing GS activity in the brain leads to selective deficits in GABAergic inhibition and global inhibition of GS leads to gross deficits in neuronal inhibition as reflected by the appearance of seizures that precede death (18,19). In accordance with these animal studies the expression levels of GS and its subcellular distribution are modified in temporal lobe epilepsy (20 -22). Given the critical role that GS plays in facilitating neurotransmission, it is of fundamental importance to understand how astrocytes regulate GS expression level. GABA B Rs are also expressed in astrocytes however their physiological significance remains elusive (23)(24)(25)(26).
In this study, we demonstrate that astrocytic GABA B Rs are important determinants of GS expression levels. Specifically, we reveal that GS binds directly to the cytoplasmic tail of the R2 subunit. This interaction determines the subcellular localization of GS and enhances its stability by limiting its proteasomal
Cell Culture and Transfection-COS-7 cells were maintained in Dulbecco's modified Eagle's medium/F12 (1:1) nutrient mix with 10% fetal bovine serum and 1% of PenStrep (Invitrogen). COS-7 cells were transfected with glutamine synthetase (GS) with empty plasmid pRK5, prK5-mycGABA B R1, pRK5␤3, and pRK5-flagGABA B R2 using electroporation (1). Astrocytes were prepared from the cortex and hippocampus of 3-day-old mouse pups of either sex as previously described (27). Cells were grown in Advanced modified Eagle's medium (MEM), containing 10% fetal bovine serum (FBS), 1 mM pyruvate, 2 mM glutamine in 5% CO 2 . Confluent cultures were shaken at 225 rpm overnight, and the medium was changed the next morning; this process was repeated a total of three times. Cells were trypsinized and cultured for 24h in 10 M cytosine arabinoside and allowed to grow to confluence. Cells were then transferred to 6 well-plates and used 48 h after transfection.
Immunoprecipitation and Metabolic Labeling-Cells were lysed in lysis buffer consisting of Tris, pH 8 20 mM, NaCl 150 mM, Triton 1%, EDTA 5 mM, NaF 10 mM, Na 3 VO 4 2 mM, and Na pyrophosphate 10 mM for 1 h at 4°C on a circular rotor. Lysates were precleared with mouse IgG attached to prot-G beads for 4 h at 4°C. Lysates were then incubated over-night with 50 l of Flag-R2 matrix. Precipitated immunocomplexes were washed, alternatively, in low salt (NaCl 150 mM) and high salt (500 mM NaCl) and very stringent buffer (NaCl 2 M and Triton 2%) and PBS 1ϫ at 4°C. Immunoprecipitated material antigen was resolved on a 8% SDS-PAGE gel and immunoblotted with Flag and/or GS antibodies and visualized using detection ECL (Super-Signal; Pierce). Signals were then quantified using a LAS-3000 imager (Fujifilm). To measure ubiquitination cells were lysed in 1% SDS, NaF 50 mM, and EDTA 1 mM and then sonicated. Lysates were then diluted with lysis buffer supplemented with 10 mM of N-ethylmaleimide and immunoprecipitated with GS antibody. Precipitated material was then immunoblotted with anti-ubiquitin and GS antibodies. For metabolic labeling, COS-7 cells were incubated in methionine-free Dulbecco's modified Eagle's medium containing 100 Ci of [ 35 S] for 30 min. Cells were then washed and incubated in complete media for up to 8 h at 37°C. GS stability was then measured using immunoprecipitation as outlined above and [ 35 S]methionine incorporation was quantified using a phospho-imager and Quantity One software (Bio-Rad). For the analysis of ubiquitination SDS-PAGE was performed under non-reducing conditions. These conditions were employed to prevent masking of GS immunoreactivity (45 kDa) by IgG heavy chain (50 kDa) on subsequent immunoblots.
Biotinylation-To isolate cell surface proteins, COS-7 cells or astrocytes were labeled with 1 mg/ml NHS-Biotin (Pierce) at 4°C for 30 min. Cultures were then lysed as outlined above, and detergent-soluble extracts were exposed to avidin beads (Pierce). Cell surface and total fractions were then subject to immunoblotting for GS, actin, or R2 antibody.
Immunofluorescence-48 h after transfection, cells were washed in PBS 1ϫ at 4°C and then fixed in PFA 4% followed exposure to 50 mM NH 4 Cl. After permeabilizing with 0.3% Triton, cells were incubated overnight with anti-glutamine synthetase mouse, anti-␤3 rabbit, and anti-R2 goat antibodies. Cells were washed and then incubated with and TRITC anti-rabbit mouse (1:1000), Cy5 anti-rabbit rabbit (1:1000), and FITC antigoat (1:1000) antibodies for 1 h at room temperature and mounted with Dako. Immunofluorescence was visualized with an invert confocal microscope (Nikon). Image acquisition was performed with NIS-Element software. Images were analyzed with ImageJ software. To measure colocalization we created regions of interest (ROI) corresponding to one cell expressing both R2 and GS. The program highlighted the colocalized points of two 8-bits images. The colocalized points appeared white by default. Two points were considered as colocalized if their respective intensities were strictly higher than the threshold of their channels. Percentage of colocalized pixels per area were then compared for R2 and GS immunoreactivity between treatments.
Membrane Fractionation-Mice hippocampi were homogenized with a glass Teflon homogenizer in ten times volume of ice-cold homogenization buffer (sucrose 0.32 M, HEPES 10 mM pH 7.4, EDTA 2 mM, EGTA 2 mM, NaF 50 mM, Na pyrophosphate 10 mM, and a mixture of protease inhibitors). Nuclei were removed by centrifugation at 1000 ϫ g for 15 min. A crude membrane fraction was then isolate via centrifugation at 17,000. Following 2 washes in the above buffer membranes were solubilized in SDS-sample buffer. Membrane and cytosol fractions were then subject to immunoblotting for GS, tubulin, or R2 antibody.
Data Analysis-Data were analyzed using GraphPad PRISM, and statistical significance was determined at p Ͻ 0.05 using one-way ANOVA followed by Dunnett's multiple comparison post hoc test or Student's t test for two groups.

RESULTS
Glutamine Synthetase Binds to the GABA B R2 Subunit-Prior mass spectroscopy analysis of GABA B Rs purified from rodent brain revealed the presence of GS, which in the adult brain is predominantly expressed in astrocytes (29). Given that glia express functional GABA B Rs, we sought to determine if these GPCRs were associated with GS, an enzyme that is predominantly localized to the cytoplasm. We compared the subcellular distribution of GABA B Rs and GS in crude membrane and cytosolic fractions prepared from rodent brain. The GABA B R2 subunit and N-cadherin were both localized to the membrane fraction while tubulin was localized to cytosolic fractions. Comparatively, nearly equivalent levels of GS were found both in the cytosolic and membrane fractions (Fig. 1A).
To assess if GS and GABA B Rs were capable of association we examined if the cytoplasmic intracellular domains of the GABA B R1 and R2 subunits were capable of binding to GS. To do this, we expressed these regions as glutathione S-transferase fusion proteins in E. coli (GST, GST/R1 and GST/R2, respectively (1). Purified fusion proteins incubated with hippocampal lysates showed significantly higher levels of GS binding to GST-R2 but not GST-R1 ( Fig. 1B Collectively, these results suggest that residues 898 -941 within GABA B R2 are critical for GS binding. To assess whether GS and R2 were capable of interacting in a cellular environment we used COS-7 cells, which do not express significant levels of either protein when compared with brain ( Fig. 2A). Therefore, we expressed GS alone or together with a FLAG-tagged version of the GABA B R2 subunit (30). Detergent soluble extracts from expressing cells were subject to immunoprecipitation with Flag beads and precipitated material was immunoblotted with GS and R2 antibodies. Under these conditions, co-immunoprecipitation of GS with R2 was evident (Fig. 2B). Our in vitro studies suggested that the interaction of GS with GABA B R2 was dependent upon residues within the cytoplasmic C terminus. Importantly, deletion of the entire C-terminal intracellular domain did not compromise either the membrane trafficking of the R2 subunit or its ability to form functional GABA B Rs on co-expression with GABA B R1 (31,32). Therefore, we examined the effects of deleting 891-941 (R2⌬CT) on the ability of GS to immunoprecipitate with R2. The deletion included the hypothetical binding site of GS (Fig. 2C) and the reported phosphorylation site of PKA to exclude its eventual effect (1). We observed that using equimolar amounts of the respective plasmids that R2⌬CT was more stable than R2. To control for differences in expression level between R2 and R2⌬CT constructs, we compared the immunoreactivity ratios of GS:R2 and GS:R2⌬CT. The ratio of GS:R2⌬CT immunoreactivity was significantly reduced to 29 Ϯ 12% compared with GS:R2 (Fig. 2C, p ϭ 0.047). Together, these results suggest that the association between GS and GABA B Rs is dependent upon residues 891-941 in the R2 subunit.
GABA B Rs Increase the Total Expression Levels of GS-To analyze the significance of GABA B Rs to GS expression we tested their role in regulating enzyme levels. Compared with cells expressing GS alone, co-expression with R2 increased GS levels to 180 Ϯ 20% of control (Fig. 3A, p ϭ 0.0032). This effect was also seen in cells expressing functional GABA B Rs composed of R1 and R2 (187 Ϯ 19% of control; p ϭ 0.0033).
To test if the ability of the R2 subunit to stabilize GS expression is shared with other proteins we examined the effects of the GABA A R ␤3 subunit on GS stability. In common with GABA B R2, GABA A R ␤3 is able to exit the endoplasmic reticulum and stably accumulate on the plasma membrane without the requirement for oligomerization with other GABA A receptor subunits or accessory proteins (33,34). In contrast to R2, co-expression with ␤3 did not significantly modify the steady state accumulation of GS (Fig. 3A, p ϭ 0.093).
The ability of GABA B Rs to interact with GS is dependent upon amino acids 891-941. Therefore we created a construct in which these resides were deleted (R2⌬CT). We observed that for the same amount of transfected DNA, R2⌬CT was more stable than R2. In order to control for variations in the expression levels of R2 and R2⌬CT we measured the ratio of GS:R2 immunoreactivity. Deletion of amino acids 891-941 significantly decreased this value to 45 Ϯ 7% of control (Fig. 3B, p ϭ  0.005). Collectively, these results suggest that GABA B Rs increase expression levels of GS, which is dependent on residues 891-941 in GABA B R2.

GABA B Rs Enhance the Targeting of GS to the Plasma Mem-
brane-To verify our immunoblotting data, we used immunocytochemistry to compare the expression levels and subcellular localization of GS when expressed alone or with GABA B Rs. To do so we compare the number of GS pixels/unit area under each condition. Compared with cells expressing GS alone co-expression with R2 increased GS levels from 41.7 to 50 Ϯ 1.2 pixel/unit area ( Fig. 4A; p ϭ 0.0001). In contrast, co-expression with the ␤3 subunit reduced this value to 23.5 Ϯ 1.7 (Fig. 4).
We noted in that in some of our images the presence of R2 appeared to lead to translocation of GS to the plasma membrane (see the arrows in Fig. 4). To further investigate this observation, cytosolic and membrane fractions were subject to immunoblotting. In cells expressing R2 there was a significant increase in the level of GS in the membrane fraction when compared with cells expressing GS alone (Fig. 5A, GS/R2; 179 Ϯ 23% of control; p ϭ 0.043). By comparing the ratio of GS:R2 and GS:R2⌬CT immunoreactivity it was evident that the accumulation of GS in the membrane fractions was dependent upon residues 891-941 in R2 (Fig. 5A, GS:R2⌬CT; 63.2 Ϯ 8% of control; p ϭ 0.021).
As an independent means of verifying this result cells we performed biotinylation experiments to compare the amount of GS at the plasma membrane in presence of either R2 or R2⌬CT. GS immunoreactivity was detected in the surface fraction with R2 and it was dependent on residues 891-941 (Fig. 5B, R2⌬CT ϭ 48 Ϯ 12% of control; p ϭ 0.019). Together, these results suggest that the association between GS and GABA B R2 subunit determines GS subcellular localization.
GABA B Rs Enhance GS Stability by Modulating Ubiquitination and Proteasomal Degradation-To assess the mechanisms by which GABA B Rs increase the steady state accumulation of GS, we compared its stability in the presence and absence of R2.  (Fig. 6A). When expressed alone 55 Ϯ 10% of GS remained after 8 h. In contrast when co-expressed with R2, 96 Ϯ 3% of GS remained (Fig. 6A, p ϭ 0.033) In Schwann cells GS is subject to ubiquitination and proteasomal degradation (35). Thus, we examined if GABA B Rs stabilize GS by regulating its ubiquitination. To do so cells expressing GS alone or with R2 were immunoprecipitated with GS antibody and immunoblotted with antibodies against polyubiquitin in addition to GS. To quantify ubiquitination gels were cut below 100 kDa to occlude IgG cross reactivity, and HRP reaction product was measured between 90 -40 kDa, and corrected for nonspecific binding subtracting values seen from non-transfected cells. Using this approach it was evident that GS ubiquitination was significantly decreased to 59 Ϯ 11% of control when expressed with R2 ( Fig. 6B; p ϭ 0.0098).
To further analyze GS degradation, expressing cells were treated with the proteasome inhibitor lactacystin for 8 h. Treatment of cells with lactacystin increased GS levels to 139 Ϯ 9% of control (Fig. 6C, p ϭ 0.004). The effects of lactacystin on GS stability were occluded by GABA B R2 (Fig. 6C). Collectively, these results suggest that GABA B Rs increase GS stability by reducing its ubiquitination and subsequent proteasomal degradation.   t test; p Ͻ 0.05, n ϭ 4). B, cells expressing GS/R2, or GS/R2⌬CT, were labeled with 1 mg/ml NHS-Biotin. Cells were then lysed and after purification on avidin the resulting cytosolic (C) and surface (S) fractions were immunoblotted with R2, GS, and actin antibodies. GS levels in surface fractions were then normalized to cells expressing R2 subunits. Data represent mean Ϯ S.E. (unpaired t test; p Ͻ 0.05, n ϭ 4). *, significantly different from control, (p Ͻ 0.05).

GABA B Rs Are Determinants of GS Expression Levels in Cul-
tured Astrocytes and the Brain-To examine the relevance of our studies in COS-7 cells, we assessed the role that GABA B Rs play in determining the stability of GS in cultured astrocytes. Previous studies have shown that cultured astrocytes express both GABA B R receptor subunits and GS (26,36). To test the significance of GABA B Rs for GS stability, we used lipofection to introduce R2 and R2⌬CT subunits into cultured astrocytes (a procedure that leads to transfection efficiencies between 10 and 30%). Similar to COS-7 cells, the level of endogenous GS expression in astrocytes transfected with R2⌬CT was reduced to 60 Ϯ 7% of control (Fig. 7A, p ϭ 0.007). In order to determine the effect of R2 subunit on GS trafficking, the transfected astrocytes were subject to biotinylation. GS was observed in the surface fraction in the presence of the R2 subunit. This effect was dependent on residues 891-941 (Fig. 7B, R2⌬CT ϭ 19 Ϯ 4%, p ϭ 0.002).
To confirm these results, we performed immunocytochemistry to compare expression levels and co-localization between R2 and GS (Fig. 7C). In R2-transfected astrocytes, there was a significant increase in the level of endogenous GS expression compared with cells expressing R2⌬CT (Fig. 7C, R2 ϭ 1.35 Ϯ 0.1, R2⌬CT ϭ 0.89 Ϯ 0.08, p ϭ 0.001, Mean Gray Value, AU). Moreover, we observed higher levels of co-localization between R2 and GS (R2 ϭ 17.47 Ϯ 2.35) compared with GS/R2⌬CT (6.97 Ϯ 1, p ϭ 0.0002). Taken together, these results suggest that GABA B R2 is a critical determinant of GS stability and subcellular localization in cultured astrocytes.
It is well accepted that the properties of astrocytes in culture are often distinct to their counterparts within the brain. There-fore, we assessed whether modifying GABA B R expression levels in the brain would influenced GS expression levels, using GABA B R2 knockout mice (R2-KO) (38). In these mice R2 expression is ablated and R1 subunit levels are greatly reduced (37). R2 homozygotes die shortly after birth and thus we measured GS expression levels in p2-4 mice R2-KO and WT littermate controls. Consistent with previously published studies, R2 subunit expression was not detected in R2-KO mice (38). Furthermore, GS expression levels were reduced in R2-KO mice to 39 Ϯ 9% of WT controls (Fig. 7D, p ϭ 0.015). These results are consistent with our studies in COS-7 cells and further support the role of the GABA B R2 subunit in determining the steady state expression levels of GS in both cultured astrocytes and the brain.

DISCUSSION
In the brain GS expression is restricted to astrocytes and other types of glia. GS plays an essential role in regulating neuronal excitability by providing neurons with glutamine the major metabolic precursor of GABA. In parallel with this GS plays an important role in limiting excitoxicity by reducing glutamate accumulation. Consistent with an essential role for GS in limiting neuronal excitability inhibition of GS activity leads to seizures and a selective reduction in the efficacy of inhibitory neurotransmission.
GS has long been considered as a cytoplasmic enzyme, but recent work has shown a vesicular-like labeling in astrocytes (39). Here we have examined the cellular mechanisms that underlie the stability and subcellular localization of GS. Our studies focused on the possible role of GABA B Rs, which are FIGURE 7. GABA B Rs regulate GS expression levels in cultured astrocytes and the brain. A, astrocytes expressing R2 or R2⌬CT were immunoblotted with R2, GS, and actin antibodies. The ratios of GS:R2 and GS:R2⌬CT immunoreactivity were determined and normalized to values for GS:R2. Data represent mean Ϯ S.E., p Ͻ 0.01; unpaired t test, n ϭ 5. B, astrocytes transfected with R2, or R2⌬CT were labeled with NHS-Biotin. After purification on avidin the resulting cytosolic (C) and surface (S) fractions were immunoblotted with R2, GS, and actin antibodies. GS levels in surface fractions were normalized to levels seen for cells expressing R2. Data represent mean Ϯ S.E., (p Ͻ 0.01; unpaired t test, n ϭ 4). C, astrocytes transfected with R2 or R2⌬CT and stained with Flag (green) and GS (red) antibodies followed by confocal microscopy; scale bar, 20 microns. Arrows indicate co-localized puncta of GS/R2 immunoreactivity, and in the lower panel co-localization as determined using ImageJ is shown in white. The level of endogenous GS in R2-positive puncta was then determined and expressed as mean gray value (p Ͻ 0.001; unpaired t test, n ϭ 4). In the lower panel, co-localization between GS and R2, or GS and R2⌬CT were determined. Data represent mean Ϯ S.E., p Ͻ 0.01; unpaired t test, n ϭ 3. D, detergent solubilized hippocampal extracts were from WT and R2KO mice were immunoblotted with R2, GS, and actin antibodies. The levels of GS were determined and normalized to values in WT mice (p Ͻ 0.05; unpaired t test, n ϭ 6). *, significantly different from control, p Ͻ 0.05; **, p Ͻ 0.01; ***, p Ͻ 0.001.
co-expressed only in astrocytes with GS in physiological conditions. Our results demonstrate that GS from brain extracts selectively binds to residues 891-941 within the intracellular domain of GABA B R2. Importantly, these residues lie outside the coil-coiled domain in R2, which is essential for dimerization with GABA B R1. Thus binding of GS to GABA B R2 will not preclude the formation of functional receptor heterodimers. To assess whether these respective proteins were associated in a cellular environment we used COS-7 cells. This initial expression system allowed us to limit possible confounds of protein overexpression, as they do not express significant levels of either protein. As revealed by our immunoprecipitation experiment, binding of GS to GABA B R2 was evident, and it was an interaction that was stable in 2 M NaCl. Consistent with our in vitro assays binding of GS to GABA B Rs as measured using immunoprecipitation was also dependent upon residues 891-941 within the R2 subunit.
To assess the physiological significance of this protein-protein interaction, we examined the effects of GABA B Rs on the stability of GS. Strikingly, co-expression with GABA B Rs increased the total expression levels of GS, a phenomenon that was dependent upon 891-941 in GABA B R2. Moreover, this effect was not replicated by co-expression with the ␤3 subunit of GABA A R, which is able to access the plasma membrane like GABA B R2. In keeping with increasing steady state expression levels, metabolic labeling indicated that GABA B Rs increased the stability of GS. Past studies in Schwann cells suggested that GS is subject to proteasomal degradation. In agreement with this, GABA B Rs decreased GS ubiquitination, and occluded the effects of proteasome inhibitors on GS stability. Collectively, these results suggest that GABA B Rs act to stabilize GS by reducing its ubiquitination and subsequent proteasomal degradation. In addition to our biochemical experiments, we used imaging to assess the effects of GABA B Rs on GS stability. This approach confirmed our biochemical measurements and also revealed that GABA B Rs may play a role in targeting GS to the plasma membrane. To further investigate this possibility, we used subcellular fractionation coupled with biotinylation. These approaches revealed that targeting of GS to the cell surface was dependent upon residues 891-941 in GABA B Rs.
As a means of assessing the relevance of our studies in COS-7 cells, we assessed the role that GABA B Rs play in regulating the stability of endogenous GS expression in cultured astrocytes. This approach revealed that the presence of R2 subunit lacking residues 891 to 941 selectively reduced total GS expression levels and its expression at the plasma membrane. Given the limitations of working with cultured astrocytes, we further assessed the effects of GABA B Rs on GS stability in the brain using R2-KO mice. Consistent with our studies in COS-7 cells and astrocytes, GS expression levels were reduced in mice deficient in GABA B R2, strongly supporting our in vitro measurements.
In summary, our studies suggest a significant role for GABA B Rs, both in determining the stability of GS and in regulating its targeting to the plasma membrane. Given the role GS plays in regulating GABAergic inhibition, this interaction may be significant to in ensure efficient glutamine synthesis to support GABA synthesis by local interneurons.