SIRT4 Protein Suppresses Tumor Formation in Genetic Models of Myc-induced B Cell Lymphoma*

Background: Glutamine metabolism is essential for Myc-induced tumors. Results: SIRT4 regulates mitochondrial glutamine metabolism and the growth and survival of Burkitt lymphoma cells independent of Myc. Conclusion: SIRT4 has a tumor-suppressive role in Myc-induced B cell lymphoma. Significance: SIRT4 may be a potential target against Myc-driven and glutamine-dependent tumors. Glutamine metabolism plays an essential role for growth and proliferation of many cancer cells by providing metabolites for the maintenance of mitochondrial functions and macromolecular synthesis. Aberrant activation of the transcription factor c-Myc, e.g. caused by t(8;14) chromosomal translocation commonly found in Burkitt lymphoma, is a key driver of cellular glutamine metabolism in many tumors, highlighting the need to identify molecular mechanisms that can suppress glutamine usage in these cancers. Recently, the mitochondrial sirtuin SIRT4 has been reported to function as a tumor suppressor by regulating glutamine metabolism, suggesting that it might have therapeutic potential for treating glutamine-dependent cancers. Here, we report that SIRT4 represses Myc-induced B cell lymphomagenesis via inhibition of mitochondrial glutamine metabolism. We found that SIRT4 overexpression can dampen glutamine utilization even in Myc-driven human Burkitt lymphoma cells and inhibit glutamine-dependent proliferation of these cells. Importantly, SIRT4 overexpression sensitizes Burkitt lymphoma cells to glucose depletion and synergizes with pharmacological glycolysis inhibitors to induce cell death. Moreover, SIRT4 loss in a genetic mouse model of Myc-induced Burkitt lymphoma, Eμ-Myc transgenic mouse, greatly accelerates lymphomagenesis and mortality. Indeed, Eμ-Myc-induced B cell lymphoma cells from SIRT4 null mice exhibit increased glutamine uptake and glutamate dehydrogenase activity. Furthermore, we establish that SIRT4 regulates glutamine metabolism independent of Myc. Together, these results highlight the tumor-suppressive role of SIRT4 in Myc-induced B cell lymphoma and suggest that SIRT4 may be a potential target against Myc-induced and/or glutamine-dependent cancers.

The MYC oncogene, which encodes a master transcription factor c-Myc (herein termed Myc), has been implicated in many human cancers by regulating cellular metabolism (1,2). Recent studies have shown that Myc coordinates the expression of genes required for glutamine catabolism and triggers cellular dependence on glutamine as an important source for biosynthetic and bioenergetic needs of cell growth (3,4). The efficient tumor formation driven by Myc amplification is well studied in human Burkitt lymphoma, in which Myc expression is dysregulated by the MYC/IgH chromosomal translocation (5). Previous studies have shown that increased glutamine metabolism is essential for survival and proliferation of Myc-induced Burkitt lymphoma cells (6). The E-Myc transgenic mouse model, which overexpresses Myc under the control of the immunoglobulin heavy chain gene enhancer (E), has constitutive Myc activation, providing an animal model to study Myc-driven lymphomas (7). These mice overexpress Myc exclusively in B cells and succumb to spontaneous pre-B and B cell lymphomas, which reach an incidence of 50% at 15-20 weeks (on a C57BL/6 background). Importantly, Myc activation/amplification-induced metabolic reprogramming triggers cellular addiction to glutamine for their growth and survival (3), highlighting the need to identify new pathways that can suppress glutamine usage even in the presence of constitutive Myc activation.
Sirtuins (SIRT1-7) are a conserved family of NAD-dependent deacetylases, deacylases, and ADP-ribosyltransferases that play essential roles in cell metabolism, stress response, and longevity (8,9). Recently, we and others reported that the mitochondrial SIRT4 exerts tumor-suppressive activities by repressing mitochondrial glutamine metabolism, in part through modification and repression of glutamate dehydrogenase (GDH) 2 (10,11). However, little is known about how SIRT4 interacts with other oncogenic pathways that promote metabolic reprogramming in cancer cells. Because Myc supports growth and proliferation of Burkitt lymphomas, at least in part, by promoting the expression of enzymes that drive glutamine metabolism, we hypothesized that SIRT4 overexpression may be a novel mechanism for repressing Myc-induced B cell lymphomas, providing important implications for suppressing glutamine utilization in Myc-driven tumors.
In this study, we examined whether SIRT4 regulates Myc-induced B cell lymphoma. Using two human Burkitt lymphoma cell lines, we demonstrated that SIRT4 overexpression represses mitochondrial glutamine metabolism and inhibits proliferation and survival of these cells. We examined the tumor modulatory role of SIRT4 for the first time using a genetic mouse model of Myc-driven lymphoma. SIRT4 loss in E-Myc transgenic mice accelerated E-Myc-induced lymphomagenesis and mortality. Mechanistically, we found that SIRT4 suppresses glutamine metabolism independent of Myc. Together, these results identify a new role for SIRT4 in suppressing Myc-induced B cell lymphoma.

EXPERIMENTAL PROCEDURES
Vectors and Virus Production-The EXPANSIN7, SIRT4, and SIRT4H161Y cDNAs were recombined into pLenti CMVTRE3G Dest plasmid (Addgene). Lentiviral supernatants were generated by transient transfection of HEK293T cells and harvested 48 h after transfection.
Cell Culture-Ramos and Raji human Burkitt lymphoma cells (kindly provided by Dr. Anthony G. Letai, Dana-Farber Cancer Institute, Boston, MA) were maintained in RPMI (Invitrogen) supplemented with 10% fetal bovine serum (Clontech) and penicillin/streptomycin (Invitrogen). Stable cell lines expressing indicated cDNAs were generated by lentiviral transduction in the presence of 8 g/ml Polybrene followed by selection with appropriate antibiotic resistance markers. Cells were induced by incubation with doxycycline (1 g/ml) (Clontech). To establish B cell lymphoma lines, lymph nodes from diseased mice were propagated in RPMI supplemented with 10% FBS, penicillin/streptomycin, and 2-mercaptoethanol (Invitrogen).
Animal Studies-Animal studies were performed according to protocols approved by the Institutional Animal Care and Use Committee, the standing committee on animals at Harvard. E-Myc transgenic mice (catalogue name, C57BL/6J-Tg(IghMyc)22Bri/J) were purchased from The Jackson Laboratory. E-Myc males were crossed with Sirt4 Ϫ/Ϫ females with the C57BL/6 background (12), and then E-Myc/Sirt4 ϩ/Ϫ male offspring were crossed with Sirt4 ϩ/Ϫ females to generate E-Myc/Sirt4 ϩ/ϩ mice and E-Myc/Sirt4 Ϫ/Ϫ mice. The expected genotypes were obtained in Mendelian ratios. The transgene was detected by PCR amplification according to the manufacturer's instructions. Animals were monitored twice weekly after birth for palpable tumors and signs of illness, and only mice showing enlarged lymphoid organs at necropsy were designated as having lymphomas.
Glutamine and Glucose Measurements-Glutamine, ammonia, glucose, and lactate levels in culture media were measured using the BioProfile FLEX analyzer (Nova Biomedical), as described previously (10). Briefly, fresh media were added to a six-well plate of cells, and metabolite levels in the media were measured at the indicated times and normalized to the number of cells in each well.
Cell Proliferation Assay-Cells were grown in complete culture media or in the absence of glucose or glutamine in 6-well plates at 10 5 cells per well for the indicated days and counted. Cell number was immediately analyzed on a Z1 Coulter Counter.
Flow Cytometric Measurement of Cell Death-Cells were harvested, pelleted by centrifugation, and resuspended in PBS containing 3% FBS. The measurement of cell death was performed by flow cytometry using propidium iodide staining, as described previously (10).
Quantitative RT-PCR-Total RNA was prepared with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. 1 g of total RNA was reverse-transcribed using iScript cDNA synthesis kit (Bio-Rad). Diluted cDNAs were analyzed by real-time PCR using SYBR Green I master mix on a LightCycler 480 (Roche Applied Science). The level of gene expression was normalized to ␤-actin. The primer sequences were: CCTTTCGCTCATACTCTACCAC and AAACACTA-CCAAGCCCAGG for human ASCT2; TCGGAAAGCTGTA-CTGGTTG and TCTGTTCCCCACAATCCAAG for human actin; CATCTACTTCCTCTTCACCCG and CCACACCAT-TCTTCTCCTCTAC for mouse Asct2; ATGGCTTTTGCGT-TTGTCTG and GAGGTATCCAAAGGTCGCTG for mouse Sn2; and AGCCATGTACGTAGCCATCC and CTCTCAGC-TGTGGTGGTGAA for mouse actin.
GDH Activity Measurement-GDH activity was measured with the GDH activity assay kit (Biovision) according to the manufacturer's instructions. Briefly, cells were counted and homogenized. The cell extracts were used for the enzyme assays, in which the NADH produced by GDH was measured with a microplate reader (Agilent Technologies).
Statistical Analysis-Unpaired two-tailed Student's t test was performed unless otherwise noted. All experiments were performed at least two or three times. For the mice survival study, the log rank (Mantel-Cox) test was performed.

SIRT4 Suppresses Mitochondrial Glutamine Metabolism in
Human Burkitt Lymphoma Cells-Recent studies by our laboratory and others have shown that SIRT4 limits glutamine anaplerosis and acts as a tumor suppressor in vitro and in vivo (10,11). The Myc oncogene promotes the expression of genes involved in metabolic reprogramming of cells toward glutaminolysis and triggers cellular dependence on glutamine for their growth and survival (4,13). However, the interaction between Myc and SIRT4 has never been investigated. Thus, we sought to probe whether SIRT4 can repress glutamine metabolism and tumorigenesis in Myc-driven tumors.
First, we examined whether elevated SIRT4 expression represses cellular glutamine metabolism in Myc-induced B cell lymphomas. As tumor cells may readily adapt their fuel utilization for growth and survival, we generated a novel doxycycline (Dox)-inducible system to acutely increase SIRT4 expression in Ramos or Raji human Burkitt lymphoma cell lines. These cells contained Dox-inducible EXPANSIN7 plant protein (pEXP7; control), human SIRT4 (SIRT4), or a catalytic mutant of SIRT4 (SIRT4H161Y) (10) constructs, such that Dox treatment resulted in a rapid induction of each protein (Fig. 1, A and B). The resulting cells provide an important new tool to assess the immediate effects of SIRT4 induction on cellular metabolism.
We observed that Dox-induced SIRT4 overexpression resulted in a pronounced reduction in glutamine consumption in both Burkitt lymphoma cell lines, whereas overexpression of pEXP7 or SIRT4H161Y did not affect fuel utilization (Fig. 1, C and D). In the mitochondria, glutamine is metabolized via glutaminase (GLS) to glutamate and ammonia (NH 4 ϩ ) and further converted to the tricarboxylic acid (TCA) cycle intermediate ␣-ketoglutarate via GDH or aminotransferases. It has been reported that in glioblastoma cells, most of the ammonia (Ͼ90%) in the media was derived from this glutamine anaplerosis (14). Indeed, we found that SIRT4-induced reduction of glutamine consumption was accompanied by a reduction in ammonia production from cells (Fig. 1, E and F). However, we observed no obvious change in glucose uptake and lactate secretion (data not shown), consistent with our previous study showing that SIRT4 directly regulates mitochondrial glutamine metabolism but not glucose consumption (10). Thus, these data demonstrate that SIRT4 overexpression represses mitochondrial glutamine metabolism in Myc-driven human Burkitt lymphoma cells.
SIRT4 Suppresses the Growth of Human Burkitt Lymphoma Cells-Refilling the mitochondrial carbon pool by glutamine anaplerosis is essential for the biosynthetic roles of mitochondria and the rapid proliferation of cancer cells (3,15). As our results showed that glutamine anaplerosis is repressed by SIRT4 overexpression in Burkitt lymphoma cells (Fig. 1), we next tested the idea that SIRT4-mediated repression of mitochondrial glutamine metabolism would inhibit the proliferation of Burkitt lymphoma cells. Indeed, we found that Dox-induced SIRT4 overexpression significantly repressed proliferation of both Burkitt lymphoma cell lines, whereas over-  FEBRUARY 14, 2014 • VOLUME 289 • NUMBER 7 expression of pEXP7 or SIRT4H161Y did not (Fig. 2, A and B). GLS is the first enzyme for mitochondrial glutamine metabolism (16), and its inhibition limits glutamine entry into the TCA cycle (6,17). It has been shown that SIRT4 overexpression no longer inhibits glutamine uptake when GLS1 is reduced (10).

SIRT4 Represses Myc-induced B Cell Lymphomagenesis
To examine whether SIRT4 represses cell proliferation through the repression of mitochondrial glutamine metabolism, we cultured SIRT4-inducible lymphoma cells with BPTES (18), an inhibitor of GLS, and measured proliferation with or without Dox treatment. Importantly, control and SIRT4-induced cells proliferated at similar rates under these conditions (Fig. 2C).
Remarkably, BPTES treatment inhibits cell proliferation more profoundly than SIRT4 overexpression, suggesting that SIRT4 functions downstream of GLS and represses proliferation of Burkitt lymphoma cells by limiting mitochondrial glutamine metabolism. Taken together, in culture, SIRT4 overexpression can repress the growth of Myc-driven tumor cells.
Because previous studies showed that the overexpression of Myc sensitizes cells to glutamine withdrawal (17), we measured the proliferation of Ramos and Raji cells under glutamine deprivation. Although Burkitt lymphoma cells sustained their growth in glucose-depleted media, the growth rates of these cells were significantly diminished with glutamine deprivation (Fig. 2D and data not shown), indicating that glutamine metab-  olism is important for cell proliferation of human Burkitt lymphoma cells. Mitochondrial glutamine metabolism supports the refilling of the mitochondrial TCA cycle in the absence of glucose, and glutamine metabolism alone can sustain cell growth in Myc-induced Burkitt lymphoma cells (6). To further examine whether SIRT4 overexpression suppressed cell proliferation through glutamine metabolism, we grew these cells in glucose-deprived media, thereby stalling glycolytic flux and forcing the cells to rely on mitochondrial glutamine metabolism for proliferation. Under these conditions, pEXP7 and SIRT4H161Y cell lines grew at the same rate with or without Dox treatment (Fig. 2, E and F). Strikingly, we observed that Dox-induced SIRT4 overexpression dramatically repressed proliferation of both Burkitt lymphoma cells (Fig. 2, E and F). Notably, the magnitude of growth inhibition caused by SIRT4 overexpression was highly increased in these conditions when compared with the inhibition under glucose-repleted media (Fig. 2, A and B). Together, these data demonstrate that SIRT4 inhibits proliferation of Myc-induced Burkitt lymphoma cells by limiting glutamine entry into the mitochondrial metabolism.
SIRT4 Synergizes with Glycolytic Inhibition to Induce the Cell Death of Burkitt Lymphoma Cells-Mitochondrial glutamine catabolism is essential for sustaining cell viability in the absence of glucose (14,19), and ectopic Myc expression drives cells to be dependent on glutamine for survival (17). To assess the functional relevance of decreased mitochondrial glutamine metab-olism by SIRT4 overexpression on the survival of Myc-driven B cell lymphoma cells, we deprived Burkitt lymphoma cells of glucose, thereby shifting cellular dependence to glutamine to maintain viability. Both Burkitt lymphoma cells were able to survive and proliferate in glucose-deficient media ( Fig. 2D and data not shown). The addition of Dox did not affect the cell survival of pEXP7-and SIRT4H161Y-overexpressing Ramos and Raji cell lines (Fig. 3, A and B). However, Dox-induced SIRT4 overexpression in both cell lines significantly induced cell death in glucose-deprived media (Fig. 3, A and B), suggesting that SIRT4 overexpression reduced the ability of Burkitt lymphoma cells to utilize glutamine for mitochondrial energy production, which is essential for cell survival.
To further test the effect of SIRT4 overexpression on the sensitivity of Burkitt lymphoma cells to glycolysis inhibition, we cultured lymphoma cells with 2-deoxyglucose, an inhibitor of glucose metabolism, and assessed the cell viability after Dox treatment. Consistent with our previous results, Dox-induced SIRT4 overexpression sensitized Burkitt lymphoma cells to 2-deoxyglucose-induced cell death, whereas overexpression of pEXP7 or SIRT4H161Y did not (Fig. 3, C and D). These data provide further support for the critical role of SIRT4 in glutamine metabolism and survival of Myc-induced Burkitt lymphoma cells and demonstrate that the combination treatments with SIRT4 overexpression and glycolysis inhibitors potently synergize to kill tumor cells with elevated Myc signaling.  FEBRUARY 14, 2014 • VOLUME 289 • NUMBER 7

JOURNAL OF BIOLOGICAL CHEMISTRY 4139
Loss of SIRT4 Accelerates Lymphomagenesis and Mortality in E-Myc Transgenic Mice-Loss of SIRT4 increased glutaminedependent cell proliferation and tumorigenesis (10). We found that SIRT4 overexpression represses glutamine utilization, proliferation, and survival of Myc-induced Burkitt lymphoma cells. To extend these cellular findings to an in vivo model, we crossed E-Myc transgenic mice with SIRT4 knock-out (KO) mice (12) to generate E-Myc/SIRT4KO mice and E-Myc/SIRT4 wild type (WT) littermates. This is the first investigation of the tumor-suppressive role of SIRT4 in a genetic mouse model of human cancer.
To examine whether the loss of SIRT4 affects tumorigenesis in this mouse model, the survival of E-Myc/SIRT4KO mice (n ϭ 26) was compared with the survival of E-Myc/SIRT4WT mice (n ϭ 24). Importantly, we found that the loss of SIRT4 significantly decreased the survival of E-Myc mice (p ϭ 0.0238 with the log rank test) (Fig. 4A). The median survival of E-Myc/SIRT4KO mice was 139 days, which was significantly shorter than E-Myc/SIRT4WT mice (195 days, p Ͻ 0.05). Although there was no significant difference between the survival of E-Myc/SIRT4 heterozygous (n ϭ 30) and E-Myc/ SIRT4WT mice, there was a slight trend that E-Myc/ SIRT4Het mice died earlier than E-Myc/SIRT4WT mice, with a median survival of 150 days (Fig. 4A). At autopsy, the spleen weights of E-Myc/SIRT4KO mice were heavier than those of E-Myc/SIRT4WT mice (Fig. 4B), consistent with the increased mortality of E-Myc/SIRT4KO mice. As we hypothesize that SIRT4 loss accelerates lymphomagenesis, we next examined lymphoma incidence in these mice at 11 and 16 weeks of age. Approximately 41% (14/34) and 71% (29/41) of E-Myc/SIRT4KO animals developed B cell lymphomas at 11 and 16 weeks, respectively, whereas 23% (7/30) and 52% (22/42) of age-matched E-Myc/SIRTWT mice developed lymphomas (Fig. 4C). None of the nontransgenic SIRT4 WT or KO mice developed lymphoma during a comparable period of monitoring (Fig. 4C). As reported previously (5), tumors arising in these mice expressed B220 but were heterogeneous with respect to the amount of IgM expression (Fig. 4D, left). We detected no differences in the distribution of these tumor types when comparing tumors from SIRT4 WT versus KO mice (Fig. 4D, right). Taken together, our data demonstrate that SIRT4 has tumorsuppressive activities in a genetic mouse model of human Burkitt lymphoma.
Next, to assess whether the mechanistic functions of SIRT4 were important in these E-Myc B cell lymphomas, we measured the usage of glutamine and glucose in the cells derived from E-Myc/SIRT4WT and E-Myc/SIRT4KO B cell lymphomas. Consistent with our previous data, SIRT4 loss increased glutamine uptake and ammonia production of these cells (Fig. 4, E and F), but not glucose uptake and lactate production (data not shown). These data support the model that SIRT4 loss enhances glutamine metabolism, contributing to the growth of B cell lymphomas. Indeed, SIRT4 KO lymphoma cells grew faster than WT cells (Fig. 4G). Thus, these results surprisingly show that even the accelerated glutamine usage in a Myc-driven tumor cell can be further induced by SIRT4 loss.
SIRT4 Regulates Cellular Glutamine Metabolism in a Mycindependent Manner-Myc regulates the transcription of diverse target genes, and it stimulates mitochondrial glutaminolysis, which is essential for its proliferative and oncogenic functions (4). It has been shown that the modulation of Myc transcriptional activity, through a dominant-interfering Myc mutant, exerts profound effects on tumor growth (20). Because SIRT4 expression levels regulate cellular glutamine metabolism even in tumor cells with elevated Myc expression, we hypothesize that SIRT4 regulates glutamine metabolism independent of Myc. To test this idea, we first examined whether Myc expression is changed by SIRT4 expression levels. We found that Myc protein levels were not affected by SIRT4 overexpression or SIRT4 loss (Fig. 5, A and B). We next assessed whether SIRT4 could repress glutamine metabolism in Myc-driven tumors by repressing the activity of Myc. It has been shown that Myc regulates glutamine metabolism by enhancing the transcription of glutamine transporters, ASCT2 and SN2 (4), and by elevating GLS expression through posttranscriptional regulation (21). When we examined the expression levels of these Myc target genes in SIRT4 WT and KO lymphoma cells, we observed no obvious change (Fig. 5, B and C), suggesting that SIRT4 does not regulate glutamine metabolism by inhibiting Myc itself. Conversely, we also assessed whether SIRT4 expression was regulated by Myc. As reported, Myc overexpression significantly induced the transcription of its target gene ASCT2, whereas SIRT4 expression was not changed (Fig. 5, D and E), demonstrating that SIRT4 is not a target of Myc in this system. SIRT4 is a negative regulator of GDH activity (12), and GDH contributes to the inhibitory role of SIRT4 in glutamine metabolism (10,11). To test whether SIRT4 regulates GDH activity in Myc-driven lymphoma cells, we measured GDH activity in cells derived from E-Myc/SIRT4WT and E-Myc/SIRT4KO B cell lymphomas. We found that GDH activity was significantly increased in SIRT4 KO lymphoma cells when compared with WT cells (Fig. 5F). Taken together, these results provide critical evidence that SIRT4 regulates mitochondrial glutamine metabolism and proliferation of B cell lymphoma cells in a Myc-independent manner and that the loss of this important regulatory node accelerates Myc-induced lymphomagenesis (Fig. 5G).

DISCUSSION
In this study, we reveal the profound impact of SIRT4 on mitochondrial glutamine metabolism and proliferation using in vitro and in vivo Myc-driven tumor models. Here we report that SIRT4 suppresses glutamine metabolism even in Myc-activated Burkitt lymphoma cells and inhibits glutamine-dependent proliferation of these cells. Importantly, SIRT4 overexpression sensitizes Burkitt lymphoma cells to glucose-free media and synergizes with glycolysis inhibitors to induce cell death. Moreover, SIRT4 loss can further activate glutamine usage and accelerate Myc-induced lymphomagenesis and mortality of E-Myc transgenic mice. These data demonstrate that SIRT4 can modulate glutamine metabolism in addition to the regulation by Myc.
Our findings are consistent with previous studies showing that SIRT4 functions as a tumor suppressor by regulating mitochondrial glutamine metabolism (10,11). Moreover, we found that SIRT4 KO mice spontaneously develop several types of tumors, including lung tumor, liver tumor, mammary tumor, and lymphoma, and that SIRT4 expression is decreased in many human cancers (10).
Here, we show that the loss of SIRT4 further accelerates Myc-induced B cell lymphomas. It is a common feature of proto-oncogenes that their oncogenic potential is accompanied with cooperating mutations. For example, the expression of   ϭ 15), and E-Myc/SIRT4KO (n ϭ 14) mice at autopsy. The boxes represent the interquartile range; whiskers represent the 10th-90th percentile range; and bars represent the median. C, cumulative lymphoma incidences at 11 and 16 weeks of age. Significance between E-Myc/SIRT4WT and E-Myc/SIRT4KO mice was determined by log rank test. p ϭ 0.0450. D, left, analysis of lymph node cell suspensions isolated from E-Myc/SIRT4WT and E-Myc/SIRT4KO mice. Dot plots show B220 and IgM expression on the cell population from independent mice that were examined after the onset of illness by using flow cytometry. Right, relative frequencies of lymphoma types of E-Myc/SIRT4WT (n ϭ 12) and E-Myc/SIRT4KO (n ϭ 13) mice: pre-B lymphoma (B220 ϩ IgM Ϫ ) and mature B lymphoma (B220 ϩ IgM ϩ ). E and F, glutamine uptake (E) and ammonia production (F) from B cell lymphoma cells from E-Myc/SIRT4WT and E-Myc/SIRT4KO mice incubated for the indicated times (n ϭ 3). Data are mean Ϯ S.E. G, growth curves of B cell lymphoma cells from E-Myc/SIRT4WT and E-Myc/SIRT4KO mice. Cell number was measured every 24 h for 4 consecutive days (n ϭ 3). Data are mean Ϯ S.D. *, p Ͻ 0.05, **, p Ͻ 0.01, and ***, p Ͻ 0.001. miR-17ϳ92 cluster, frequently amplified in human cancer, is required for Myc-induced B cell lymphoma by counterbalancing the pro-apoptotic activity of Myc (22). Thus, SIRT4 loss may create a tumor-permissive environment to provide additional metabolic advantages, supporting tumor cell survival and proliferation.
Many therapeutic strategies have been developed to target metabolic pathways of cancer cells (23). For example, targeting glucose metabolism through glycolysis inhibitors has been used to treat tumors (24,25). However, cancer cells retain the capacity for metabolic adaptation to survive periods of diminished glucose metabolism by activating alternative metabolic path- ways (23), and some cancer cells depend on a high rate of glutaminolysis for their continued growth and survival. For instance, Myc-driven tumor cells display addiction to glutamine metabolism (3) and can sustain their viability and proliferation under glucose-depleted conditions (6,21). We also showed that Myc-induced human Burkitt lymphoma cells are able to survive and proliferate in glucose-deficient media. Importantly, SIRT4 overexpression sensitizes these cells to glucose withdrawal-induced cell death and significantly inhibits glucose-independent proliferation. Moreover, the combination of SIRT4 overexpression and glycolysis inhibitors significantly increased the death of lymphoma cells. Thus, our current work highlights the therapeutic potential of SIRT4 for targeting metabolic flexibility and adaptation of Myc-driven tumors.
Previous studies have shed light on the importance of mitochondrial glutamine metabolism for oncogene-induced tumorigenesis, and the significance of SIRT4 in this node is just beginning to emerge. It has been shown that glutamine catabolism by the TCA cycle is essential for Kras-mediated tumorigenicity (26). Also, the inhibition of mitochondrial glutamine metabolism by targeting GLS represses Rho GTPases-induced transformation (27). Recently, Csibi et al. (11) found that mammalian target of rapamycin complex 1 (mTORC1) promotes mitochondrial glutamine metabolism by transcriptionally repressing SIRT4 expression and that SIRT4 overexpression reduces proliferation and transformation of tuberous sclerosis 2 mutant cells. In this study, we uncover the profound impact of SIRT4 on mitochondrial glutamine metabolism and tumor survival and proliferation in glutamine-dependent Myc-driven tumor models. Thus, these findings suggest that the tumorsuppressive activity of SIRT4, by regulating mitochondrial glutamine metabolism, could apply to other oncogene-induced tumors and provide a new avenue for tumor therapy.