Identification and Characterization of CD300H, a New Member of the Human CD300 Immunoreceptor Family*

Background: CD300 family molecules regulate innate immune responses. Results: We identified and characterized a novel immunoreceptor, CD300H. Conclusion: CD300H mediates neutrophil chemoattractant production. Significance: CD300H may play an important role in innate immunity. Recruitment of circulating monocytes and neutrophils to infection sites is essential for host defense against infections. Here, we identified a previously unannotated gene that encodes an immunoglobulin-like receptor, designated CD300H, which is located in the CD300 gene cluster. CD300H has a short cytoplasmic tail and associates with the signaling adaptor proteins, DAP12 and DAP10. CD300H is expressed on CD16+ monocytes and myeloid dendritic cells. Ligation of CD300H on CD16+ monocytes and myeloid dendritic cells with anti-CD300H monoclonal antibody induced the production of neutrophil chemoattractants. Interestingly, CD300H expression varied among healthy subjects, who could be classified into two groups according to “positive” and “negative” expression. Genomic sequence analysis revealed a single-nucleotide substitution (rs905709 (G→A)) at a splice donor site on intron 1 on either one or both alleles. The International HapMap Project database has demonstrated that homozygosity for the A allele of single nucleotide polymorphism (SNP) rs905709 (“negative” expression) is highly frequent in Han Chinese in Beijing, Japanese in Tokyo, and Europeans (A/A genotype frequencies 0.349, 0.167, and 0.138, respectively) but extremely rare in Sub-Saharan African populations. Together, these results suggest that CD300H may play an important role in innate immunity, at least in populations that carry the G/G or G/A genotype of CD300H.

Recruitment of blood leukocytes to sites of infection is essential for host defense against infection. Circulating monocytes (Mo) 2 and neutrophils are especially important effectors in the initiation of inflammatory responses to microbes (1). Mo in the human peripheral blood are divided into subsets on the basis of their cell surface expression of CD14 and CD16 (2). A major subset with a phenotype of CD14 ϩ CD16 Ϫ is referred to as classical or inflammatory Mo (iMo); these are similar to Ly6C hi CCR2 hi Mo in mice (3). The CD16 ϩ Mo subset can further be divided into two subsets, namely CD14 ϩ CD16 ϩ and CD14 dim CD16 ϩ , on the basis of CD14 expression (4). CD14 dim CD16 ϩ Mo resemble Ly6C low Cx 3 CR1 hi Mo in mice and are referred to as patrolling Mo (pMo) (5)(6)(7). pMo crawl along the luminal side of the vascular endothelium in the steady state. CD14 ϩ CD16 ϩ Mo are referred to as intermediate monocytes (intMo) and are found at low frequency (ϳ5% of blood monocytes) (8) in the peripheral blood; they represent a continuous transition from iMo (CD14 ϩ CD16 -) to pMo (CD14 dim CD16 ϩ ) (7,9). iMo produce TNF-␣, IL-6, and IL-1 in response to bacteria-associated signals. In contrast, pMo respond poorly to bacterial components but potently to viruses and nucleic acids; they thus selectively detect viral infection and injury and produce inflammatory cytokines (4). In mice, Ly6C low pMo provide immune surveillance and rapidly extravasate before neutrophils in response to Listeria monocytogenes infection (5). In addition, activation of intravascular Ly6C low pMo is responsible for neutrophil recruitment via TLR7-dependent CXCL1 production (6). However, the precise mechanism by which pMo produce chemokines, particularly in humans, remains unclear. CD300 family molecules are type 1 immunoreceptors belonging to the immunoglobulin superfamily and are encoded by seven genes on human chromosome 17 and nine genes on mouse chromosome 11 (10,11). They are expressed on myeloid lineage cells, including monocytes-macrophages, granulocytes, dendritic cells, and mast cells, suggesting that they play an important role in innate immunity. CD300A (also named MAIR-I (12), LMIR1 (13), and CLM-8 (14) in mice) and CD300LF (MAIR-V (15,16), LMIR3 (17)(18)(19)(20), and CLM-1 (21) in mice) mediate inhibitory signals via the immunoreceptor tyrosine-based inhibitory motif in their cytoplasmic regions. By contrast, CD300LB (LMIR5 (22)), CD300C, CD300LD (MAIR-IV (23), LMIR4 (17,24), ), and CD300E have short cytoplasmic tails with no signaling motifs. However, CD300LB and CD300E each contain a positively charged lysine * This work was supported in part by grants provided by the Ministry of Education, Culture, Sports, Science, and Technology of Japan. The authors declare that they have no conflicts of interest with the contents of this article. The nucleotide sequence(s) reported in this paper has been submitted to the GenBank TM /EBI Data Bank with accession number(s) LC013475 and LC013476. 1 To whom correspondence should be addressed. residue, whereas CD300C and CD300LD contain a negatively charged glutamic acid in their transmembrane domains (10) (11). These receptors noncovalently associate with membranebound signaling adaptor proteins, including DNAX adaptor protein 12 (DAP12), DAP10, and the ␥ chain of the Fc receptor for IgE (Fc⑀RI␥) through interaction with a negatively charged amino acid (aspartic acid) in the transmembrane domain of the adaptors and thus transmit activating signals (10,11). DAP12 and Fc⑀RI␥ contain an immunoreceptor tyrosine-based activation motif in their cytoplasmic regions, whereas DAP10 contains a YXXM motif, a potential Src homology 2 domain-binding site for the p85 regulatory subunit of the PI3K. Among the seven genes encoding human CD300 molecules, those encoding CD300A, CD300LB, CD300C, CD300E, CD300LF, and CD300LG each have mouse homologs, as determined from their functional, molecular, and genetic characteristics (10,11). However, the mouse counterpart of human CD300LD and the human counterpart of mouse MAIR-II (CLM-4/LMIR2) and MAIR-VI (CLM-3/LIMR7) are unclear.
Here, we identified a previously unannotated gene encoding a novel molecule, designated CD300H, in the CD300 family gene cluster on human chromosome 17. We demonstrate that CD300H is expressed on CD14 ϩ CD16 ϩ intMo and CD14 dim CD16 ϩ pMo and associates with DAP12 or DAP10. Upon cross-linking of CD300H on CD16 ϩ Mo and CD11c ϩ dendritic cells (DCs), it mediates an activating signal for the production of neutrophil chemoattractants.
Generation of Fc Fusion Protein-Fusion proteins of the entire extracellular domains of CD300H, CD300A, and CD300C with the Fc portion of human IgG1 (CD300H-Fc, CD300A-Fc, and CD300C-Fc, respectively) were generated as described previously (26).
Phylogenic Analysis-The protein sequences of human CD300 family molecules were aligned by using the ClustalW program (28). NJplot was used to construct tree diagrams (29).
Cells and Transfectants-Murine T cell lines 2B4 and BW5147, human monocytic cell lines THP-1 and U937, and human embryonic kidney cell line 293T were used; they were cultured in RPMI 1640 medium containing 5% fetal calf serum (FCS) except for 293T cells. 293T cells were cultured in DMEM containing 10% FBS. Blood samples were collected from healthy donors at the University of Tsukuba under an institutional review board-approved protocol. Written informed consent was obtained from the donors. Human peripheral blood mononuclear cells (PBMCs) were isolated from whole blood with LymphoPrep (Axis-Shield, Oslo, Norway) in accordance with the manufacturer's protocol. CD16 ϩ Mo were isolated from PBMCs by using the MACS cell separation system (Miltenyi Biotec); NK cells were depleted from the PBMCs by using anti-CD56 MicroBeads, and then CD16 ϩ Mo were purified from the NK cell-depleted PBMCs by using anti-CD16 MicroBeads. The purity of the CD16 ϩ Mo was more than 85%, as determined by flow cytometry. CD16 Ϫ Mo were isolated from PBMCs by using the MACS cell separation system (Miltenyi Biotec); CD16 ϩ cells were depleted from the PBMCs by using anti-CD16 MicroBeads, and then CD16 Ϫ Mo were purified from the CD16 ϩ cell-depleted PBMCs by using anti-CD14 MicroBeads. The purity of the CD16 Ϫ Mo was more than 90%, as determined by flow cytometry. CD11c ϩ DCs were isolated from PBMCs by using the Myeloid dendritic cell isolation kit (Miltenyi Biotec). The purity of the CD11c ϩ DCs was more than 95%, as determined by flow cytometry. 2B4, U937, and BW5147 transfectants stably expressing CD300H, CD300A, or CD300C tagged with FLAG or HA at the N terminus were established, as described previously (12). 293T transfectant transiently expressing FLAG-tagged CD300Hs was established by using Lipofectamine 2000 (Invitrogen).
SNP Genotyping-Genomic DNA was extracted and purified from peripheral blood leukocyte using the QuickGene 610 L system (Fujifilm, Tokyo, Japan). Polymorphism was analyzed by genomic PCR with primer pair 5Ј-AAACCCAGAAGAGGC-CAGAG-3Ј and 5Ј-GGTTTGGACCTTGACTGTGC-3Ј and subsequent direct sequencing with the Big Dye Termination Kit (Applied Biosystems, Foster City, CA).
cDNA Synthesis and RT-PCR-Total RNA was extracted with Isogen reagent (Nippon Gene, Tokyo, Japan), and cDNA was synthesized by using a High-Capacity RNA-to-cDNA Kit (Applied Biosystems, Foster City, CA). Real-time RT-PCR was performed with SYBR Green master mix (Applied Biosystems). Expression of each target gene was normalized against that of GAPDH (primer sequences 5Ј-CTTCACCACCATGGAGAA-GGC-3Ј and 5Ј-GGCATGGACTGTGGTCATGAG-3Ј).
Neutrophil Migration Assay-Neutrophils were isolated from whole blood with Polymorphprep (Axis-Shield) in accordance with the manufacturer's protocol. Supernatant obtained from TX93-stimulated CD16 ϩ Mo was added to the lower compartment of 96-well Transwell plates (pore size 3.0 m; Corning, Inc.) at a total volume of 235 l/well. Neutrophils (1 ϫ 10 5 ) were placed in the upper compartment at a total volume of 75 l, and then the plates were incubated for 30 min at 37°C in 5% CO 2 . Cells in the lower compartment were collected and counted by using a Guava easyCyte Mini flow cytometer (Millipore).
Statistical Analyses-The unpaired Student's t test was used for statistical analyses. p values of Ͻ0.05 were considered statistically significant. All statistical analyses were carried out using GraphPad Prism version 5.0c software (GraphPad Software, San Diego, CA).

Results
Cloning of the Gene Encoding Human CD300H-In our search for a human homolog of MAIR-II in the National Center for Biotechnology Information database, we found a previously unannotated gene located in the human CD300 gene cluster on chromosome 17 (Fig. 1A). We isolated full-length cDNA from human CD14 ϩ Mo-derived cDNA by RT-PCR. Sequence analysis revealed that an open reading frame of 603 bp encoded a type I transmembrane receptor, which contained a 20-amino acid (aa) leader sequence, a 148-aa extracellular region containing an Ig-like domain, a 23-aa transmembrane region, and a 10-aa cytoplasmic domain with no signaling motif (Fig. 1B). The Ig-like domain contained a potential N-glycosylation site. The transmembrane domain contained a positively charged lysine residue (Fig. 1C). Because this homolog was the eighth member of the human CD300 family of receptors, we termed it CD300H. We also identified a splicing variant that lacked a transmembrane domain; we called this variant CD300Hs (Fig. 1, B and C). A database search demonstrated that CD300H had 44% amino acid identity with mouse MAIR-II/CLM-4/LMIR2 and CD300c/CLM-6, but it had lower rates of identity with CD300e/CLM-2/LMIR6 (42%), CLM-3/LMIR7 (35%), CD300ld/CLM-5/LMIR4 (40%), CD300lb/CLM-7/LMIR5 (35%), and CD300lg/CLM-9 (35%) among the non-immunoreceptor tyrosine-based inhibitory motif-bearing receptors. The gene encoding CD300H was located between CD300C and CD300LD on human chromosome 17q25.1 (Fig. 1A). We analyzed the degree of identity of the extracellular Ig domain of CD300H with the sequences of CD300 family members and constructed a molecular phylogenetic tree by using ClustalW2 phylogeny. The Ig-like domains of CD300A and CD300C had the greatest identity (53%) with that of CD300H; other members of the family had lower identity levels (Fig. 1D).
Expression of CD300H-To investigate the cellular distribution of CD300H, we generated a mAb against CD300H (clone TX93) and analyzed its specificity. TX47 and TX49 were previously generated mAbs against CD300C and against CD300A and CD300C, respectively (Fig. 2, B and C) (27). Although CD300H had a high level of identity with CD300A and CD300C, TX93 mAb bound specifically to CD300H-expressing THP-1 and 2B4 transfectants but not to CD300A-or CD300Cexpressing BW5147 transfectants (Fig. 2, A and B). In addition, TX93 mAb bound to CD300H-Fc fusion protein but not to CD300A-Fc or CD300C-Fc protein (Fig. 2C). These results indicated that TX93 mAb specifically recognized CD300H.
Individual Differences in CD300H Expression-Genomic DNA database analysis demonstrated that CD300H consisted of four exons (Fig. 4A), but the gene encoding CD300Hs lacked exon 3, which encoded the transmembrane region. Interestingly, we observed that 4 of the 13 individuals examined in Fig.  3 showed no detectable expression of CD300H on CD16 ϩ Mo (Fig. 3H, open circles). Genomic DNA analyses of these individuals demonstrated a single-nucleotide substitution (rs905709; G3 A) of CD300H, which abolished the intron 1 donor splice site (Fig. 4A). This can lead to loss of CD300H transcript expression through the action of nonsense-mediated mRNA decay machinery (30). Indeed, persons carrying the rs905709 A/A genotype showed a loss of CD300H transcripts (Fig. 4B) and protein (Figs. 3H and 4C). In contrast, CD16 ϩ Mo derived from subjects with a G/A or G/G genotype expressed the CD300H transcript (Fig. 4B) and protein on their cell surfaces (Figs. 3H and 4C). The A/A genotype frequency of rs905709 differed significantly in the different ethnic populations and is observed at high frequency in Han Chinese in Beijing, China (HCB), Japanese in Tokyo, Japan (JPT), and CEPH (Utah Residents with Northern and Western European Ancestry, CEU) populations (A/A genotype frequencies 0.349, 0.167, and 0.138, respectively). However, it is extremely rare in Yoruba in Ibadan, Nigeria (YRI) (Fig. 4D) (31).
CD300H Associates with DAP12 and DAP10 in U937 Cells-To analyze the biochemical characteristics of CD300H, we generated a BW5147 transfectant stably expressing FLAG-tagged CD300H at the N terminus. Immunoprecipitation of CD300H protein from lysates of BW5147 transfectant and subsequent immunoblotting with anti-FLAG antibody revealed that human CD300H had a molecular mass of ϳ25 kDa under reducing conditions and ϳ50 kDa under non-reducing conditions (Fig. 5A), suggesting that CD300H forms dimer formation. To determine whether CD300H forms homodimeric or heterodimeric structure, we generated BW5147 transfectant simultaneously expressing FLAG-tagged and hemagglutinin (HA)-tagged CD300H and performed a co-immunoprecipitation assay. We found that HA-tagged CD300H was not co-immunoprecipitated with FLAG-tagged CD300H and vice versa (Fig. 5B), suggesting that CD300H forms heterodimeric structure with an undetermined molecule with a molecular mass of ϳ25 kDa expressed in BW5147 cells.
Next, we analyzed to determine whether CD300Hs is a secreted form of CD300H. We performed immunoblot analysis of culture supernatant of 293T transfectant transiently expressing CD300Hs tagged with FLAG at the N terminus and found that CD300Hs was a secreted form of CD300H (Fig. 5C).
CD300H possesses a lysine residue in the transmembrane region, suggesting that CD300H associates with DAP12, DAP10, and/or Fc⑀RI␥ chain. We found that FLAG-tagged CD300H was co-immunoprecipitated with endogenous DAP12 and DAP10, and vice versa, but not with Fc⑀RI␥ chain, in U937 cells (Fig. 5D). This result indicates that, unlike mouse MAIR-II, CD300H associates with DAP12 but not with the Fc⑀RI␥ chain in U937 cells.
CD300H Transduces an Activating Signal in CD16 ϩ Mo-Our findings that CD300H was expressed on CD16 ϩ Mo and was associated with DAP12 and DAP10 in U937 cell line suggested that CD300H was an activating receptor on CD16 ϩ Mo. Human CD16 ϩ Mo consists of two distinct subsets. CD14 dim CD16 ϩ Mo resemble murine Ly6C low Mo (5), which are designated pMo and produce chemokines. On the other hand, CD14 ϩ CD16 ϩ Mo are designated as intMo (32), main producers of inflammatory cytokines, such as TNF-␣ and IL-1␤ (33). Therefore, we investigated whether CD300H mediated signaling for inflammatory cytokine and chemokine production in CD16 ϩ Mo. We sorted CD16 ϩ Mo from the PBMCs of healthy donors with the G/G (CD300H ϩ ) or A/A genotype (CD300H Ϫ ) and then stimulated them with plate-coated F(abЈ) 2 fragments of TX93 mAb or control Ig (Fig. 6, A and B). Stimulation of CD300H ϩ CD16 ϩ Mo with TX93 mAb induced significantly more TNF-␣ and IL-6 production than did stimulation with control Ig. In contrast, stimulation with TX93 mAb did not increase cytokine production by CD300H Ϫ CD16 ϩ Mo compared with stimulation with control Ig (Fig. 6, B and C). Thus, CD300H transduces an activating signal for cytokine production in CD16 ϩ Mo. A recent study has demonstrated that murine Ly6C low Mo recruit neutrophils in a TLR7-dependent manner to mediate focal necrosis of endothelial cells (6). CD16 ϩ Mo produce chemoattractants for neutrophils. To examine whether CD300H is involved in this process, CD16 ϩ Mo were stimulated with plate-coated F(abЈ) 2 fragments of TX93 mAb or control Ig for 24 h; the culture supernatant was then plated in the lower compartment of a Transwell, and neutrophils derived from the peripheral blood were added to the upper compartment. In the case of CD300H ϩ CD16 ϩ Mo but not CD300H Ϫ CD16 ϩ Mo, the average number of neutrophils that migrated from the upper to the lower compartment upon TX93 mAb stimulation was significantly greater than that with control Ig-stimulated culture supernatant (ϳ1.3-fold increase; p Ͻ 0.05) (Fig. 6D). These results suggested that CD300H mediated a signal for the production of a neutrophil chemoattractant. Indeed, the expression of CXCL8, CXCL1, CXCL2, and CXCL5, which encode chemokines for neutrophil attraction, was significantly greater in CD300H ϩ Mo, but not CD300H Ϫ Mo, than in the controls after stimulation with plate-coated F(abЈ) 2 fragments of TX93 mAb (Fig. 6E).

CD300H Had No Effect on IL-8 Production in CD16
Ϫ Mo-Because CD16 Ϫ Mo also expresses CD300H (Fig. 3E), we analyzed the CD300H activity on CD16 Ϫ CD14 ϩ classical Mo sorted from the peripheral blood from donors with the G/G (CD300H ϩ ) or A/A genotype (CD300H Ϫ ). We analyzed the production of cytokines, including CXCL8 (IL-8), IL-12p70, TNF-␣, IL-10, IL-6, and IL-1␤, after stimulation with anti-CD300H mAb by using cytometric bead array. However, we could not detect a significant amount of cytokines except for IL-8 and observed no significant differences in IL-8 production between stimulation with control antibody and anti-CD300 mAb (Fig. 6, F and G). This might be the result of the low level of CD300H expression on CD16 Ϫ Mo compared with CD16 ϩ Mo.
CD300H Transduces an Activating Signal in CD11c ϩ DC-CD11c ϩ DCs also express CD300H at a level comparable with CD16 ϩ Mo. We addressed whether CD300H transduces activating signal in CD11c ϩ DCs. We purified CD11c ϩ cells by the MACS sorting system (Fig. 7A), stimulated them with platecoated F(abЈ) 2 fragment of TX93 mAb, and analyzed surface expression of co-stimulatory molecule and cytokine production by flow cytometry. We found that TX93 stimulation did not enhance the expression level of co-stimulatory molecules, such as CD40, CD86, CD80, and HLA-DR, on the G/G genotype (CD300H ϩ )-derived CD11c ϩ DCs (Fig. 7B). However, CD11c ϩ DCs from G/G genotype, but not A/A genotype (CD300H Ϫ ), produced IL-8 upon CD300H stimulation (Fig.  7C). These results indicate that CD300H induced IL-8 production by CD16 ϩ Mo and CD11c ϩ DCs and may contribute to neutrophil attraction.

Discussion
We identified and characterized a new member of the human CD300 family of immunoreceptors, which we designated CD300H. It is expressed on CD14 ϩ CD16 ϩ intMo and CD14 dim CD16 ϩ pMo in the peripheral blood and transduces an activating signal for the production of inflammatory cyto- kines (TNF-␣ and IL-6) and chemokines (CXCL1, CXCL2, CXCL5, and IL-8) by CD16 ϩ Mo. In addition, CD300H induces IL-8 production by CD11c ϩ DC. Importantly, we demonstrated that CD300H mediates neutrophil recruitment through the production of chemoattractants for those cells, suggesting that CD300H plays an important role in host immunity against bacterial and viral infections. Despite recent progress in human Mo studies, the physiological roles of CD16 ϩ Mo have not been fully elucidated. One recent report described a healthy family with CD16 ϩ Mo deficiency in the blood (34), suggesting that that the function of CD16 ϩ Mo is redundant. In accordance with this idea, we also found a subpopulation of healthy people whose CD16 ϩ Mo did not express CD300H, suggesting that CD300H on CD16 ϩ Mo is functionally redundant. However, because CD16 ϩ pMo are implicated in inflammatory diseases, such as arthritis and atherosclerosis (35)(36)(37), or autoimmune diseases, such as systemic lupus erythematosus (4), deficiency of CD16 ϩ pMo or CD300H on CD16 ϩ pMo might be potentially advantageous, rather than disadvantageous, for maintaining homeostasis. The genetic mutation known as CCR5-⌬32 is a well studied example of an advantageous allele with a well characterized geographic distribution. This mutation plays an important role in human immunodeficiency virus (HIV)-1 resistance because it prevents functional expression of the CCR5 chemokine receptor, the entry receptor for HIV-1 (38). The mutation is found principally in Europe and Western Asia, where average frequencies are ϳ10%, but not in Western and Central Africa (38). The mutant allele, which can be regarded as a natural knockout in humans, is not accompanied by an obvious phenotype in homozygous individuals, although CCR5 has a protective role against L. monocytogenes infection in mice (39). Additional studies are required to determine the functional role of CD300H in immunity and inflammation.
Our studies suggested that CD300H may form a heterodimeric structure in mouse T cell lymphoma, BW5147. Recent evidence demonstrated that CD300C forms either a homo- dimer or heterodimer with all CD300 family members. Although these dimer formations require Ig-like domains, CD300C did not bind other Ig-like receptors, such as TREM-1 and CD28 (44) (45). The Ig-like domain of CD300H has 53% homology to that of CD300C, suggesting that CD300H may also form heterodimer with mouse CD300 family members expressed on the BW5147 cell line. Because we did not detect surface expression of mouse CD300a, MAIR-II, and CD300lf on BW5147 cells by flow cytometry using TX41 (12), TX52 (46), and TX70 (15) mAbs, respectively (data not shown), CD300H may interact with mouse CD300 family members other than these Ig-like receptors. Unlike CD300C, CD300H has a positively charged lysine residue in its transmembrane region and interacts with DAP12 and DAP10. Thus, CD300H may be able to mediate an activating signal upon ligand binding. However, the heterodimer of CD300H with other CD300 receptors may positively or negatively modulate the signaling.
To elucidate the physiological roles of CD300H on CD16 ϩ pMo in vivo, the counterpart of CD300H in mice needs to be identified. Although we identified CD300H in our search for a human homolog of MAIR-II, there are several differences between MAIR-II and CD300H. First, MAIR-II inhibits B-cell receptor-mediated signaling in B cells (47), whereas CD300H expression on human peripheral blood B cells was not detected by flow cytometry using TX93 mAb (Fig. 3). Second, MAIR-II is a unique receptor that binds to either DAP12 or Fc⑀RI␥; however, CD300H did not show an association with Fc⑀RI␥ in U937 cells. Third, CD300H is located adjacent to CD300C on human chromosome 17, whereas Cd300ld/MAIR-IV/LMIR4/Clm-5, but not MAIR-II, is located adjacent to Cd300c on mouse chromosome 11. Recent studies have demonstrated that CD300 family receptors recognize lipids, such as phosphatidylserine, ceramide, and lipoprotein, as ligands that mediate signals in mast cells and macrophages via these receptors (48 -50). We and others reported that human CD300A binds to phosphatidylserine and phosphatidylethanolamine (11,27,50). Because CD300H has high amino acid identity with CD300A, we assessed whether CD300H also binds to phosphatidylserine and phosphatidylethanolamine by dot blot analyses using a lipid-bound membrane. However, CD300H bound to neither phosphatidylserine nor phosphatidylethanolamine (data not shown). Future studies are required for identification of a ligand for CD300H to understand the function of CD300H in vitro and in vivo.
Author Contributions-K. N. conducted the experiments and analyzed the data. S. T.-H. designed the experiments, analyzed the data, and wrote the paper. E. N. analyzed the data. A. S. supervised the overall project and wrote the paper.