Phosphorylation of Ezrin-Radixin-Moesin-binding Phosphoprotein 50 (EBP50) by Akt Promotes Stability and Mitogenic Function of S-phase Kinase-associated Protein-2 (Skp2)*

Background: The PDZ scaffolding protein EBP50 promotes Akt-dependent cell proliferation through the S-phase kinase associated protein-2 Skp2. Results: Akt phosphorylates threonine 156 of EBP50, allosterically promoting the binding of Skp2 to the first PDZ domain of EBP50. Conclusion: The interaction with EBP50 regulates the localization and stability of Skp2 and promotes cell proliferation. Significance: These studies define a regulatory mechanism contributing to Akt-dependent cell proliferation. The regulation of the cell cycle by the ubiquitin-proteasome system is dependent on the activity of E3 ligases. Skp2 (S-phase kinase associated protein-2) is the substrate recognition subunit of the E3 ligase that ubiquitylates the cell cycle inhibitors p21cip1 and p27kip1 thus promoting cell cycle progression. Increased expression of Skp2 is frequently observed in diseases characterized by excessive cell proliferation, such as cancer and neointima hyperplasia. The stability and cellular localization of Skp2 are regulated by Akt, but the molecular mechanisms underlying these effects remain only partly understood. The scaffolding protein Ezrin-Binding Phosphoprotein of 50 kDa (EBP50) contains two PDZ domains and plays a critical role in the development of neointimal hyperplasia. Here we report that EBP50 directly binds Skp2 via its first PDZ domain. Moreover, EBP50 is phosphorylated by Akt on Thr-156 within the second PDZ domain, an event that allosterically promotes binding to Skp2. The interaction with EBP50 causes cytoplasmic localization of Skp2, increases Skp2 stability and promotes proliferation of primary vascular smooth muscle cells. Collectively, these studies define a novel regulatory mechanism contributing to aberrant cell growth and highlight the importance of scaffolding function of EBP50 in Akt-dependent cell proliferation.

The regulation of the cell cycle by the ubiquitin-proteasome system is dependent on the activity of E3 ligases. Skp2 (S-phase kinase associated protein-2) is the substrate recognition subunit of the E3 ligase that ubiquitylates the cell cycle inhibitors p21 cip1 and p27 kip1 thus promoting cell cycle progression. Increased expression of Skp2 is frequently observed in diseases characterized by excessive cell proliferation, such as cancer and neointima hyperplasia. The stability and cellular localization of Skp2 are regulated by Akt, but the molecular mechanisms underlying these effects remain only partly understood. The scaffolding protein Ezrin-Binding Phosphoprotein of 50 kDa (EBP50) contains two PDZ domains and plays a critical role in the development of neointimal hyperplasia. Here we report that EBP50 directly binds Skp2 via its first PDZ domain. Moreover, EBP50 is phosphorylated by Akt on Thr-156 within the second PDZ domain, an event that allosterically promotes binding to Skp2. The interaction with EBP50 causes cytoplasmic localization of Skp2, increases Skp2 stability and promotes proliferation of primary vascular smooth muscle cells. Collectively, these studies define a novel regulatory mechanism contributing to aberrant cell growth and highlight the importance of scaffolding function of EBP50 in Akt-dependent cell proliferation.
The ubiquitin-proteasome system is a major regulator of the cell cycle (1,2). One of the critical E3 ligases involved in cell cycle progression is the Skp1/Cul-1/Rbx-1/Skp2 complex. Within this complex, S-phase kinase-associated protein 2 (Skp2) 2 is the substrate recognition subunit that binds the cell cycle inhibitors p21 cip1 and p27 kip1 and promotes their ubiquitylation and proteasomal degradation (3)(4)(5)(6). Skp2 overexpression is observed frequently in disease states associated with aberrant cell proliferation. Among these, the pro-oncogenic action of Skp2 is the best characterized. Increased Skp2 expression is detected in various types of cancer (including prostate, breast, ovarian, and lung cancers, and lymphomas (7)(8)(9)(10)(11)) and correlates with poor prognosis (11,12).
Similarly, Skp2 has profound effects during vascular remodeling. After the surgical resolution of an atherosclerotic plaque by angioplasty, local increases in growth factors and cytokines initiate cell cycle entry, often leading to restenosis. Skp2 activity is essential for the development of intimal hyperplasia (13,14). Skp2 expression is low in healthy uninjured arteries but increases rapidly upon vascular injury and promotes vascular smooth muscle cell (VSMC) growth (14,15). Conversely, Skp2 inhibition leads to significant reduction of neointimal lesions (16). These findings underscore the central role for Skp2 in vascular pathologies (17) but little is known on the mechanisms regulating Skp2 expression during vascular remodeling (13).
EBP50 is a PDZ-domain scaffolding protein that regulates mineral ions homeostasis and microvilli formation (18 -22). We reported that EBP50 expression increases in restenotic vessels upon endoluminal injury (23,24) and participates in inti-mal hyperplasia. In EBP50 Ϫ/Ϫ mice we observed an 80% reduction in neointima formation compared with WT mice following wire injury (23). Unexpectedly, we found that EBP50 promotes vascular smooth muscle cells (VSMC) proliferation by regulating the stability of Skp2. Thus, Skp2 levels are reduced in EBP50 Ϫ/Ϫ VSMC, resulting in increased expression of the cell cycle inhibitor p21 cip1 , both in primary cells and in vivo (23). These observations suggest that EBP50 is a critical regulator of Skp2 and consequently cell proliferation. Yet, the mechanisms by which this regulation occurs are not known. Here we describe a novel phosphorylation-dependent interaction between Skp2 and EBP50 that controls Skp2 localization, stability, and function.
[L424A]Skp2 mutant construct was also made by mutagenesis from Flag-tagged Skp2 (a gift from Dr. Michele Pagano, New York University School of Medicine). All plasmid DNA sequences were confirmed by sequence analysis (GeneWiz).
Cell Culture and Transfection-Primary VSMC were isolated from murine thoracic aortic explants and cultured in Dulbecco's modified eagle media (DMEM) containing 10% fetal bovine serum (FBS) in 5% CO 2 at 37°C. All experiments were performed with cells between passages 3 and 15. Cells were transfected with siRNA for Skp2 (0.1 M) using DharmaFECT Duo transfection reagent (Dharmacon, Thermo Scientific) and used for experiments 72 h after transfection. YFP-tagged and Flagtagged EBP50 constructs, Flag-tagged Skp2, and constitutively active Myr-Akt (a gift from Dr. Daniel Altschuler, University of Pittsburgh School of Medicine) were introduced in primary VSMC (1 ϫ 10 6 ) by electroporation using an AMAXA electroporator and the Basic Nucleofect kit for primary smooth muscle cells (Lonza). CHO cells were cultured in Ham's F-12 medium supplemented with 10% FBS. EBP50 constructs and Myc-Akt were transfected in CHO cells using Fugene6 (Promega).
Western Blot Analysis-Cells were lysed in urea lysis buffer (4 M urea, 62.5 mM Tris, 2% SDS, 1 mM EDTA) containing a protease inhibitor mixture. The cell lysates were resolved by SDS-PAGE. Proteins were transferred onto nitrocellulose membranes, which were then subjected to two sequential incubations with appropriate primary antibodies (1:500 dilution for EBP50, p21 cip1 , and 1:1000 dilution for Skp2 (all from Santa Cruz Biotechnology); 1:1000 dilution for p27 kip1 , pAkt, Akt antibodies (Cell Signaling); 1:5000 dilution for ␤actin (Sigma)) and horseradish peroxidase-conjugated anti-mouse or antirabbit IgG antibody (1:2000, Cell Signaling). Immunoreactivity was detected by incubation with Immune-Star ECL (Bio-Rad). Quantitation of band intensity was performed with the Image J software (National Institutes of Health). For co-immunoprecipitation assay, cells were lysed in RIPA buffer (Santa Cruz Biotechnology) containing protease inhibitor mixture. Lysates were incubated with the indicated antibodies overnight and with protein A/G beads for 2 h. Immuno-bead complexes were washed twice with Nonidet P-40 buffer (1 M Tris-base, 150 mM NaCl, 5 mM EDTA, and 0.5% Nonidet P-40). Bound proteins were then released with 2ϫ Laemmli sample buffer with 5% ␤-mercaptoethanol.
In-gel Overlay Assay-Cell lysates were immunoprecipitated with anti-Skp2 antibody or anti-HA (negative control). Immunoprecipitated protein were separated by SDS-PAGE and transferred to nylon membranes. Membranes were overlaid with His-tagged EBP50 protein (1 g/ml) and incubated with anti-His-HRP-conjugated antibody (Amersham Biosciences, 1:4000). Interactions of EBP50 with Skp2 were visualized by chemiluminescence.
Peptide Synthesis-The synthesis of the 22-amino acid carboxyl-terminal peptide of Skp2a was carried out by solid phase methodology using standard Fmoc (N-(9 fluorenyl)methoxycarbonyl) chemistry (0.1 mmol scale) on an Applied Biosystems AB433 peptide synthesizer. After synthesis, the peptidyl resin was treated overnight with 4 Eq of carboxytetramethylrhodamine in the presence of HBTU/HOBt/DIEA. Following standard trifluoroacetic acid cleavage, the product was purified by HPLC on a Vydac C-18 reverse phase column and lyophilized. The final product was characterized by electron spray mass spectrometry.
Fluorescence Polarization Assay-Purified recombinant EBP50 or the isolated PDZ domains (PDZ1 and PDZ2) in PBS were added to 96-well polystyrene plates. Rhodamine-labeled Skp2 peptide was added to each well at a final concentration of 2 M. Fluorescence polarization was measured at an excitation of 544 nm and emission at 595 nm. Recordings were performed at room temperature under equilibrium conditions.
Nuclear Magnetic Resonance-All spectra were acquired on a Bruker Avance III 700 MHz equipped with a TCI cryoprobe. Samples for the EBP50 PDZ1/Skp2 peptide interaction study contained 300 M Skp2 carboxyl-terminal 22 amino acids peptide (NKKNQEIWGIKCRLTLQKPSCL) and 50 M EBP50 PDZ1 (amino acids 1-140), in 25 mM sodium phosphate, 50 mM NaCl, 0.1 mM TCEP, 0.02% NaN 3 , 5% v/v D 2 O, at pH 6.8. 1 H, 15  Molecular Dynamics Simulations-The starting structure for molecular dynamics simulations was generated using Chimera (26). The structures of EBP50 PDZ1 (PDB ID: 1G9O) and the PDZ of PSD95 bound to the peptide KKETPV (1TP3) were overlaid. The 5 C-terminal residues of Skp2 were then superimposed to the KKETPV peptide. Structures were energy minimized for 50,000 steps (0.1 ns), followed by 5,000,000 steps (10 ns) of molecular dynamics (2 fs step size) at 310 K and 1.013 bar. Analysis of molecular dynamics data were carried out in VMD 1.9.1 and Chimera 1.6.1.
Fractionation of Nuclear or Cytoplasm Proteins-Cells were harvested by trypsinization and washed twice with ice-cold PBS followed by resuspension of the cell pellet in hypotonic buffer A (10 mM HEPES-K ϩ pH 7.5, 10 mM KCl, 1.5 mM MgCl 2 , 0.5 mM DTT) in the presence of protease inhibitor mixture. Cells were pelleted by spinning at 1,000 rpm for 5 min. The cells were lysed in ice-cold 0.5% Nonidet P-40 containing buffer A on ice for 10 min. The nuclei were isolated by centrifugation at 3,000 rpm for 2 min. The supernatant (containing the cytoplasmic protein) was collected and frozen at Ϫ80°C. The nuclear pellets were washed with buffer A (without Nonidet P-40), followed by resuspension in buffer C (20 mM HEPES-K ϩ pH 7.9, 420 mM NaCl, 0.2 mM EDTA, 1.5 mM MgCl 2 , 0.5 mM DTT, 25% glycerol). Nuclei were incubated on ice for 30 min, and vortexed periodically. Supernatants containing nuclear protein were collected by spinning at 13,000 rpm for 10 min at 4°C and then snap frozen for further use. Antibodies against histone H3 and tubulin were used as nuclear and cytoplasmic markers, respectively.
Phosphorylation Assays-In vitro. Recombinant active Akt was incubated with 10 Ci [ 32 P]ATP and 1 g recombinant EBP50. Reactions were incubated at 30°C and phosphorylation was visualized by autoradiography. For fluorescence polarization assays, recombinant Akt and EBP50 were incubated in the absence or presence of ATP. In cells. CHO cells transfected with Flag-tagged WT or [T156A]EBP50 were stimulated with 5% FBS, lysed, and immunoprecipitated with anti-Flag antibodies. Phosphorylation was visualized by anti-AKT substrate antibody (RXRXXpS/pT).
Proliferation Assays-Cells on 24-well plates were transfected as indicated and cultured until 70 -80% confluent. Cells were incubated with 1 Ci/ml [ 3 H]thymidine in the culture media for 18 h at 37°C, rinsed with PBS and exposed to 10% trichloroacetic acid (TCA) for 10 min. TCA was removed and the cell monolayers dissolved in 1 N NaOH for the determination of radioactivity. For BrdU incorporation experiments, cells were grown on coverslips and incubated in the presence of 100 M BrdU for 16 h. After fixation with 4% paraformaldehyde, cells were permeabilized in 0.5% Triton X-100/PBS for 30 min and incubated with anti-BrdU antibody (1:100, Biodesign International) in blocking solution (10% promega RQ1 DNaseI, 1% BSA, 1% FBS in PBS) at 37°C for 1 h. Flag-EBP50 was visualized with anti-Flag antibody (Sigma, 1:1000) in the same buffer. Cells were rinsed in PBS, incubated with secondary antibody anti-sheep Alexa 594 (1:1000, Molecular Probes) for BrDU or anti-rabbit Alexa 488 (1:1000, Molecular Probes) for EBP50 in PBS containing 1% BSA, 1% FBS.

RESULTS
Skp2 Binds the PDZ1 Domain of EBP50 -EBP50 contains two tandem PDZ domains: PDZ1 (residues 11-97) and PDZ2 (residues 149 -236). PDZ domains typically recognize signature sequences at the extreme C terminus of target proteins. Class I PDZ domains interact with the sequence [S/T]-X-⌽, where X is any amino acid and ⌽ is a hydrophobic residue. Class II PDZ domains preferentially interact with the sequence X-⌽-X-⌽. Examination of the Skp2 sequence revealed a C-terminal class I (PSCL; in human, primates and mouse) or class II (KPSL; in rat) PDZ-binding motif (Fig. 1A). Consistent with our previous report (23), we found that Skp2 interacts with EBP50 in coimmunoprecipitation experiments (Fig. 1B). This interaction is dependent on the PDZ ligand of Skp2 because it was abrogated by mutation of the C-terminal leucine (Leu-424) to alanine (Fig.  1B). We then performed a series of additional experiments to further characterize the interaction between Skp2 and EBP50. In-gel overlay of purified His 6 -tagged EBP50 to immunoprecipitated Skp2 established that the interaction is direct (Fig.  1C). Fluorescence polarization experiments using a Rhodamine-labeled peptide comprising the last 22 amino acids of Skp2 confirmed the direct interaction with EBP50 (Fig. 1D). Identical binding was observed for the isolated PDZ1 domain, whereas we detected no interaction with the PDZ2 domain (Fig.  1D). We then characterized the details of the interaction between Skp2 and the first PDZ domain of EBP50 by NMR. 1 H, 15 N correlation spectra (HSQC) of PDZ1 both free (Fig. 1E, in black) and bound to the C-terminal 22 amino acids of Skp2 (Fig. 1E, in red) were acquired. Clear perturbations of the resonances of G25 and F26 of PDZ1 (residues comprising the "core" GYGF binding motif) were observed upon binding of the C-terminal Skp2 peptide. The molecular model of the Skp2 peptide bound to PDZ1 confirms that the experimental observations are consistent with a canonical binding mode of the C-terminal region of Skp2 to first PDZ domain of EBP50 (Fig. 1F). Collectively, these experiments show that the C-terminal region of Skp2 interacts directly with the first PDZ domain of EBP50.
Akt Phosphorylates EBP50 and Increases the Interaction with Skp2-The proliferative effect of EBP50 in VSMC is Akt-dependent (23). We therefore hypothesized that Akt activity affects the interaction between EBP50 and Skp2. Co-immunoprecipitation assays showed that expression of a constitutively active myristolated form of Akt (HA-Myr-Akt) increased the interaction between EBP50 and Skp2 ( Fig. 2A). Interestingly, we detected HA-Myr-Akt in the same EBP50 immunoprecipitates, suggesting that phosphorylation of EBP50 by Akt may favor the formation of the complex with Skp2. This possibility was further supported by the presence of a conserved Akt consensus motif (RXRXXT/S, where X is any amino acid) comprising threonine 156 (Thr-156) as a putative phosphorylation site (Fig. 2B). In vitro phosphorylation assays showed that recombinant Akt phosphorylates purified EBP50 (Fig. 2C). To demonstrate that Akt phosphorylates EBP50 in vivo, we used CHO cells expressing Flag-EBP50. The levels of EBP50 expressed under these conditions were approximately three times higher than primary VSMC (Fig. 2D), and were similar to those observed under inflammatory conditions (27) and in vessels (23,24). Cells were incubated with FBS after overnight serum starvation to induce Akt activation (Fig. 2E, third panel). Following immunoprecipitation, EBP50 phosphorylation was detected with an antibody specific for phosphorylated Akt consensus motif (Akt PM) (Fig. 2E, upper panels). Furthermore, the Akt inhibitor AKTI prevented FBS-induced phosphorylation of EBP50 and mutation of Thr-156 to alanine abolished phosphorylation (Fig. 2F). Next, the effect of EBP50 phosphorylation by Akt on the interaction with Skp2 was determined by fluorescence polarization. As shown in Fig. 2G, the affinity of Skp2 for EBP50 increased 10-fold upon phosphorylation. Thus, Akt phosphorylates EBP50 on Thr-156 and enhances the interaction with Skp2.
EBP50 Regulates the Cellular Localization of Skp2-The C-terminal domain of EBP50 (called EBD) interacts strongly with cytoskeletal components such as ezrin, radixin and moe- sin. In both primary VSMC and CHO cells EBP50 localization is primarily determined by the cytoskeleton and we found no evidence of EBP50 in the nucleus (Fig. 3, panels C and D). We therefore speculated that the interaction with EBP50 may cause a re-localization of Skp2 from the nucleus to the cytoplasm. To test this hypothesis we performed cell fractionation studies in CHO cells (that do not express EBP50 endogenously) and in primary VSMC from WT and EBP50 Ϫ/Ϫ mice. In both cell types EBP50 significantly increased the fraction of Skp2 in the cytoplasm relative to that in the nucleus (Fig. 3, A and B). Complementary studies using confocal microscopy in primary EBP50 Ϫ/Ϫ VSMC electroporated with mCherry-EBP50 or control mRFP and immunostained for endogenous Skp2 confirmed these observations (Fig. 3C). To determine if phosphor-ylation of T156 in EBP50 by Akt affects Skp2 localization we used primary VSMC from EBP50 Ϫ/Ϫ mice expressing either WT or [T156A]EBP50. As shown in Fig. 3D, the fraction of cells with nuclear Skp2 was greater when [T156A]EBP50 was expressed than in EBP50-expressing cells. We then used a similar approach to determine the specific domains in EBP50 that mediate Skp2 localization. Expression of either WT or an EBP50 mutant with inactivating mutations in the core binding motif (GYGF to GAGA) of PDZ2 (S2) reduced the fraction of cells with nuclear Skp2 (Fig. 3E). In contrast, expression of a truncated form of EBP50 lacking the C-terminal EBD (⌬EBD) or of EBP50 constructs were either PDZ1 (S1) or both PDZ1 and 2 (S1S2) are mutated had no effect on Skp2 localization (Fig. 3E). Collectively, these experiments show that the interac- tion between EBP50 and Skp2 causes a redistribution of Skp2 from the nucleus to the cytoplasm. This effect requires both the first PDZ domain and the EBD of EBP50 and is dependent on the Akt-mediated phosphorylation of Thr-156.
The Interaction with EBP50 Increases Skp2 Stability and Function-Cellular Skp2 levels are controlled by the ubiquitinproteasome system. Skp2 is actively degraded by the Cdh1/APC complex, which is principally located in the nucleus (28,29).
Our previous experiments indicate that, upon phosphorylation by Akt, EBP50 induces relocalization of Skp2 from the nucleus to the cytosol (Fig. 3). We therefore tested the hypothesis that Akt-dependent phosphorylation of EBP50 increases Skp2 stability and function. The stability of Skp2 was determined in primary EBP50 Ϫ/Ϫ VSMC expressing control empty vector (pcDNA3), WT EBP50, or the phosphorylation-resistant [T156A]EBP50. We found that Skp2 was significantly more stable in cells expressing EBP50 than either empty vector or [T156A]EBP50 (Fig. 4A). The degradation of Skp2 in these cells was inhibited by MG132 (Fig. 4B), supporting that proteasomal degradation mediates the effect of EBP50 on cellular Skp2 levels. Consistent to our previous report (23), expression of EBP50 in VSMC increased proliferation (Fig. 4C). In contrast, no effect was observed for [T156A]EBP50 (Fig. 4C). Of note, the mitogenic effect of EBP50 was entirely dependent on Skp2, because it was abrogated in cells in which Skp2 expression was reduced by siRNA (Fig. 4C). These experiments indicate that Akt-phosphorylated EBP50 increases Skp2 stability and mitogenic action. To determine if the interaction with EBP50 directly affects the function of Skp2 we overexpressed WT Skp2 and the mutant [L424A]Skp2 that does not bind EBP50 (Fig. 1B). As shown in Fig. 4D, both Skp2 constructs were equally effective in inducing degradation of p21 cip1 and p27 kip1 , two well-characterized targets of Skp2. Also, overexpression of both WT and [L424A]Skp2 increased VSMC proliferation (Fig. 4E). Thus, EBP50 is not directly required for substrate recognition or function of Skp2-containing SCF E3 ligases.

DISCUSSION
The mechanisms underlying Akt-dependent Skp2 stabilization are incompletely understood. Other mechanisms have been proposed to explain the Akt-dependent re-localization of Skp2 in cancer cells. Both direct phosphorylation by Akt (30,31) and p300-mediated acetylation (32) of specific residues within the nuclear localization signal of Skp2 have been identified and proposed to regulate the nuclear-cytoplasmic trafficking as well as the ubiquitylation activity of Skp2. Yet, these mechanisms remain controversial (33)(34)(35) and do not explain the effect of EBP50 on Skp2 (23). The experiments reported here support a novel mechanism for the Akt-dependent regulation of Skp2. A schematic of the proposed model is shown in Fig. 5. Akt phosphorylates EBP50 on Thr-156, which is located in PDZ2 only a few residues removed from the core binding groove (GYGF). However, the C terminus of Skp2 interacts with PDZ1 of EBP50, and inactivating mutations in PDZ1, but not in PDZ2, prevent EBP50-dependent re-localization of Skp2. These data suggest that phosphorylation of Thr-156 exerts an allosteric effect on PDZ1, leading to increased affinity for Skp2. Consistent with this model is the observation that a human mutation in the Akt consensus motif of EBP50 (R153Q) significantly decreases the interaction with the sodium/phosphate transporter Npt2a which, like Skp2, binds exclusively to PDZ1 (25).
Degradation of Skp2 by the Cdh1/APC complex occurs in the nucleus (29). We show here that the interaction with EBP50 causes cytoplasmic accumulation of Skp2 leading to its stabilization. A key element of this mechanism is the ability of EBP50 FIGURE 3. EBP50 regulates the subcellular localization of Skp2. A, nuclear fractionation. CHO cells were transfected with EBP50, and either Flag-Skp2 or vector (pcDNA3) as indicated. Nuclear and cytoplasmic fractions analyzed for Flag, EBP50, H3 (nuclear marker) and Tubulin (cytoplasmic marker) by Western blot. Graph shows the average ratio (cytoplasm/nuclear) of Skp2 in cells with pcDNA3 or EBP50 overexpression from 4 -5 different experiments. *, p Ͻ 0.05. B, Skp2 levels in nuclear (N) and cytoplasmic (C) fractions of primary VSMC from WT and EBP50 Ϫ/Ϫ mouse. C, primary VSMC from EBP50 Ϫ/Ϫ mice were electroporated with mCherry-EBP50 or control mRFP and immunostained for endogenous Skp2 (in green) and EBP50 (in red). DAPI was used for nuclear staining (in blue). D, primary VSMC from EBP50 Ϫ/Ϫ mice were electroporated with Flag-tagged WT or [T156A]EBP50 and immunostained for endogenous Skp2 (in red) and Flag (in green). Nuclei are visualized with DAPI (in blue). Graph shows the percentage (Ϯ S.E.) of transfected cells with nuclear Skp2. *, p Ͻ 0.0003, n ϭ 3. E, left, schematic representation of EBP50 and mutant forms lacking the ezrin-binding domain (⌬EBD) and harboring inactivating mutations (GYGF to GAGA) in PDZ1 (S1), PDZ2 (S2), or both PDZ domains (S1S2). Right, primary VSMC from EBP50 Ϫ/Ϫ mice were electroporated with the indicated Flag-tagged EBP50 mutants and immunostained as in D. The graph shows the percentage (Ϯ S.E.) of transfected cells with nuclear Skp2. *, p Ͻ 0.05; **, p Ͻ 0.01 n ϭ 6.

EBP50 Stabilizes Skp2
to interact with cytoskeletal components (such as ezrin, radixin, and moesin) via its C-terminal EBD. Thus, a form of EBP50 lacking EBD was unable to induce cytoplasmic localization of Skp2. Notably, in the cell systems used in this work (VSMC and CHO cells) EBP50 is localized predominantly along the cytoskeleton, and we did not observe EBP50 in the nucleus.
The increased stability of Skp2 in EBP50 expressing cells leads to increased degradation of p21 cip1 and cell cycle entry (23). However, our data indicate that the Akt-dependent interaction with EBP50 has no effect on the ability of Skp2 to function within the E3 ligase complex. This is not surprising because in vitro reconstitution of the ubiquitylation activity of Skp2containing SFC ligases requires neither Akt nor EBP50 (3). However, because EBP50 regulates the cellular localization of Skp2 it is possible that it may affect the selectivity of the substrates that undergo degradation. It has been shown that cytoplasmic Skp2 induces degradation of membrane-bound E-cadherin (32) and phosphorylated (i.e. cytoplasmic) FOXO1 (36) thus regulating cell motility, proliferation and survival. Further studies are required to determine the effect of EBP50 on substrate selection by Skp2-containing E3 ligases.
The regulation of Skp2 by EBP50 has important consequences in the responses of the vasculature to surgical interventions. The expression of Skp2 increases rapidly in balloon-injured rat carotids where it regulates VSMC proliferation (16). Interestingly, a similar increase in EBP50 expression occurs during neointima formation in both rats and mice (23,24), suggesting that the concomitant expression of Skp2 and EBP50 contributes to the aberrant mitogenic response. Our data indicate that EBP50 acts upstream of Skp2 in regulating the mitogenic response of VSMC to injury. Interestingly, it has been recently shown that in breast cancer cells Akt undergoes Skp2dependent K-63-linked ubiquitylation (37). This modification is non-proteolitic and leads to Akt activation and cell proliferation. It is possible that this mechanism may also be involved in the mitogenic actions of EBP50 in the vasculature, since we  Phosphorylation of Thr-156 in the PDZ2 domain of EBP50 by Akt allosterically promotes the interaction of Skp2 with PDZ1. Because EBP50 strongly interacts with cytoskeletal proteins this interaction induces cytoplasmic localization of Skp2 and increases Skp2 stability, ultimately leading to cell cycle progression.
have found decreased Akt activation and proliferation in EBP50 Ϫ/Ϫ VSMC and because VSMC proliferation is dependent on Akt (23).
In addition to the effect on cell proliferation, EBP50 promotes VSMC motility (38) and the production of pro-inflammatory mediators in macrophages and of adhesion molecules in VSMC and endothelial cells (27). Collectively, these studies support the notion that EBP50 is a critical regulator of vascular remodeling. The definition of the molecular events and structural determinants by which EBP50 regulates Skp2 provides both the rationale and specific targets for the development of EBP50 inhibitors for the treatment of inflammatory and proliferative diseases.