SUMOylation Regulates Polo-like Kinase 1-interacting Checkpoint Helicase (PICH) during Mitosis*

Background: Mitotic SUMOylation is critical for faithful chromosome segregation in eukaryotes. Results: PICH (Plk1-interacting checkpoint helicase) is a SUMO-interacting protein and a mitotic SUMO substrate. Conclusion: PICH can be regulated by binding to SUMOylated proteins and through its own SUMOylation at mitotic centromeres. Significance: The regulation of PICH by SUMO interaction and SUMOylation reveals a novel role for mitotic SUMOylation in centromeric chromatin organization. Mitotic SUMOylation has an essential role in faithful chromosome segregation in eukaryotes, although its molecular consequences are not yet fully understood. In Xenopus egg extract assays, we showed that poly(ADP-ribose) polymerase 1 (PARP1) is modified by SUMO2/3 at mitotic centromeres and that its enzymatic activity could be regulated by SUMOylation. To determine the molecular consequence of mitotic SUMOylation, we analyzed SUMOylated PARP1-specific binding proteins. We identified Polo-like kinase 1-interacting checkpoint helicase (PICH) as an interaction partner of SUMOylated PARP1 in Xenopus egg extract. Interestingly, PICH also bound to SUMOylated topoisomerase IIα (TopoIIα), a major centromeric small ubiquitin-like modifier (SUMO) substrate. Purified recombinant human PICH interacted with SUMOylated substrates, indicating that PICH directly interacts with SUMO, and this interaction is conserved among species. Further analysis of mitotic chromosomes revealed that PICH localized to the centromere independent of mitotic SUMOylation. Additionally, we found that PICH is modified by SUMO2/3 on mitotic chromosomes and in vitro. PICH SUMOylation is highly dependent on protein inhibitor of activated STAT, PIASy, consistent with other mitotic chromosomal SUMO substrates. Finally, the SUMOylation of PICH significantly reduced its DNA binding capability, indicating that SUMOylation might regulate its DNA-dependent ATPase activity. Collectively, our findings suggest a novel SUMO-mediated regulation of the function of PICH at mitotic centromeres.

SUMOylation is a posttranslational modification that leads to diverse biological consequences. The addition of small ubiquitin-like modifier (SUMO) 3 affects target proteins by altering their subcellular localization, enzymatic activity, and/or protein-protein/protein-DNA interactions (1)(2)(3)(4)(5). Similar to ubiquitylation, SUMO conjugation to its substrates requires a cascade of three enzymes: an activating E1 enzyme, a conjugating E2 enzyme, and typically a SUMO E3 ligase enzyme. All SUMO modifications utilize the same E1 (Uba2/Aos1) and E2 (Ubc9) enzymes, but different E3 enzymes have been identified that show specificity to certain targets (6). Multiple lines of evidence show that SUMOylation is a vital posttranslational modification to ensure the proper segregation of chromosomes during mitosis (7,8). Consistent with observed segregation defects, many mitotic proteins have been identified as SUMO targets (7).
On mitotic chromosomes, a major SUMO signal is observed at the centromeric region in both somatic cells and Xenopus egg extract (XEE) cell-free assay (9,10). Using the XEE assays, we have previously identified two major PIASy-dependent mitotic chromosomal SUMO2/3 substrates: DNA topoisomerase II␣ (TopoII␣) and poly(ADP-ribose) polymerase 1 (PARP1) (11,12). TopoII␣ was one of the first mitotic SUMOylated substrates identified in budding yeast and vertebrates (11,13) and is pivotal for DNA decatenation to separate sister chromatids during chromosome segregation. Accumulating evidence indicates that SUMOylation is important for the regulation of TopoII␣ activity (14,15). Another robust mitotic SUMOylation substrate, PARP1 (12), is a member of the PARP family that catalyzes the formation of poly(ADP-ribose) on target proteins, leading to multifaceted biological consequences (16). Although we have previously shown potential PARP1 activity regulation by SUMOylation on mitotic chromosomes (12), the comprehensive mitotic role of PARP1, as well as how SUMO modification affects the function of PARP1 during mitosis, has not yet been determined.
SUMO modification often provides a new site for proteinprotein interactions (17)(18)(19), and non-covalent interactions between SUMO-interacting motif (SIM)-containing proteins and SUMOylated proteins have been shown to produce multi-ple critical functional consequences (20 -22). To extend our understanding of the downstream effects of SUMOylation at mitotic centromeres, we intended to identify SUMOylation-dependent binding protein(s) using PARP1 as bait. We identified Polo-like kinase 1 (Plk1)-interacting checkpoint helicase (PICH), which is also known as ERCC6-like protein and belongs to the SNF2 family of ATPases, as a novel SUMO-interacting partner. Prior studies have shown that PICH is essential for the proper segregation of chromosomes during mitosis (23)(24)(25). In this study, we detected PICH as a novel SUMO substrate on mitotic chromosomes. In vitro SUMOylated PICH showed reduced DNA binding capability, implicating the SUMO-dependent regulation of PICH activity. Altogether, we propose a novel regulation of PICH function at mitotic centromeres by SUMOylation.

EXPERIMENTAL PROCEDURES
Plasmids and Antibody Preparation-Human PICH (PICH hs ) cDNA was amplified from a plasmid obtained from Addgene (plasmid 41163: Nigg CB62) (23) and subcloned into pPIC3.5K fused to calmodulin-binding protein and with a T7 tag (14). PICH hs cDNA for mRNA expression was cloned into the pTGFC70 plasmid, a generous gift from Dr. Funabiki, and utilized for mRNA expression as described previously (26). Partial cDNAs for Xenopus laevis PICH were obtained by PCR amplification from X. laevis cDNA based on expressed sequence tag clone sequences that are homologous to PICH hs . The obtained partial PICH xl cDNAs were subcloned into pET28a and pMalc5x for recombinant protein expression.
Xenopus Egg Extract Immunofluorescence and Immunoblotting-Low-speed extracts arrested in metaphase by cytostatic factor (CSF) from X. laevis egg and sperm nuclei were prepared using standard protocols (27). An interphase extract was obtained by releasing CSF upon the addition of CaCl 2 to the CSF extracts (27). The mitotic chromosomes used for the immunofluorescence analysis were prepared as described previously (9). Mitotic SUMOylation was inhibited by the addition of a dominant-negative form of Ubc9 (dnUbc9) at a concentration of 150 ng/l before the induction of mitosis. The localization of human PICH on mitotic chromosomes was observed by GFP signals from exogenously expressed human PICH-EGFP mRNA in the extract (26). The DNA was stained with Hoechst 33342 dye (EMD Millipore), and the samples were mounted using VECTASHIELD (Vector Laboratory). The specimens were analyzed using a Nikon TE2000-U microscope with a Plan Apo 100ϫ/1.40 objective, and the images were taken with a Retiga SRV CCD camera (QImaging) using the Volocity imaging software (Improvision).
Chromatin isolation for immunoblotting was performed as described previously (11). Recombinant SUMO2GG-EGFP protein (100 ng/l extract) was added for the supershift assay shown in Fig. 2d. The samples were subjected to immunoblotting with the indicated antibodies.
Pulldown Assay-Pulldown assays were performed as described previously, with a few modifications (28). Non-SUMOylated and SUMOylated bait proteins were bound to either anti-T7-agarose or S-protein-agarose (EMD Millipore), depending on the affinity tag fused to the bait protein. XEE was diluted three times with immunoprecipitation buffer (20 mM NaP i , pH 7.8, 18 mM ␤-glycerol phosphate, pH 7.5, 5 mM MgCl 2 , 100 mM NaCl, 1 mM CaCl 2 , and 10 M ZnCl 2 ) and clarified by centrifugation at 25,000 ϫ g for 30 min at 4°C. The soluble fraction was further diluted with an equal volume of ChIP buffer (immunoprecipitation buffer containing 0.2% Triton X-100 and 0.2% Tween 20) and then incubated with proteinbound beads for 1 h at 4°C. SENP2-CD digestion was performed by incubating ϳ50 ng/l SENP2-CD with beads in ChIP buffer for 30 min at 25°C. Half of the volume of 3ϫ SDS-PAGE sample buffer was added to the SENP2-CD-digested samples, resolved by SDS-PAGE on 8 -16% gradient gels (Life Technologies), and analyzed by silver staining and/or immunoblotting, as indicated. For the pulldown analysis of PICH hs , either the egg extract expressing full-length PICH hs -EGFP or 15 g of purified PICH hs in ChIP buffer containing 5% gelatin was incubated with the bait. For SUMO2 pulldown, GFP-SUMO2 and GFP-SUMO2 ϫ 4 (GFP-SUMO chain) were incubated with GFP trap magnetic beads (Bulldog Bio Inc.). The pulled down samples were analyzed as described above using silver staining and immunoblotting, as indicated.
Electrophoretic Mobility Shift Assay-Non-SUMOylated PICH hs was prepared via in vitro SUMOylation with SUMO2-G, a non-conjugatable form of SUMO2. Linearized pBluescript DNA (ϳ25 nM or 50 nM) was incubated in a 10-l in vitro SUMOylation reaction containing PICH hs (ϳ1 M) for 20 min on ice. The entire reaction was separated on a 0.8% agarose gel in pre-chilled buffer at 25 V for 3 h. The DNA was visualized via ethidium bromide staining (post run). Quantification of the non-shifted DNA was performed using the ImageJ and Kaleida-Graph software.
PARylation Assay-The pulled down fractions with non-SUMOylated and SUMOylated PARP1 were used for a PARylation assay, as described previously (12). The beads were incubated with SUMOylation buffer containing NAD ϩ , and the samples were subjected to an immunoblotting analysis of PARylation with an anti-PAR antibody.

SUMOylation of PARP1 Mediates Protein-Protein
Interactions-We previously showed that PARP1 SUMOylation does not alter its enzymatic activity but does result in subtle differences in the PARylation profile of mitotic chromosomal proteins (12). To examine whether the SUMOylation of PARP1 could affect its substrate specificity, we performed a PARylation reaction with a PARP1 pulldown fraction using XEE. Either SUMOylated or non-SUMOylated PARP1 was captured on agarose beads, and the beads were then incubated with XEE to isolate binding proteins. The beads were incubated in a reaction buffer containing NAD ϩ to initiate PARylation by PARP1. Consistent with our previous finding, PARP1 showed robust self-PARylation activity regardless of SUMOylation (Fig. 1a,  lanes 1 and 2); however, we observed a difference in the PARylation profile when we compared PARylation on beads between samples without XEE and samples after pulldown with XEE using PARP1 and SUMOylated PARP1 (Fig. 1a, lanes 3 and  4). In the pulldown samples incubated with XEE, there was an increase in the PARylation of ϳ150 kDa for non-SUMOylated PARP1, whereas more PARylation, ϳ250 kDa, was observed for SUMOylated PARP1 (Fig. 1a). This finding suggests that PARP1 promotes the PARylation of its interacting proteins and that the SUMOylation of PARP1 could alter its substrate specificity in XEE. Conjugation of SUMO to cellular proteins is known to create new docking sites for protein-protein interactions through a SIM (29). Our results are consistent with this notion because SUMOylation potentially regulates the interacting proteins of PARP1. To examine this further, we sought to identify the binding proteins for PARP1 and SUMOylated PARP1. We utilized a PARP1 N-terminal 650-amino acid fragment (PARP1-N650) that contains most of the SUMOylation sites that we identified by in vitro SUMOylation with a series of mutants (data not shown). Because the expected binding proteins will be larger than 100 kDa, the use of PARP1-N650, the molecular mass of which is ϳ85 kDa with the affinity tag, will be beneficial for distinguishing the bands of the bound proteins by SDS-PAGE. In addition, we introduced SENP2 digestion after pulldown to eliminate the bands of SUMOylated bait for better visibility of the bound proteins (Fig. 1b). As shown in Fig. 1c, PARP1 bound to many proteins in XEE, and several proteins were specific for SUMOylated PARP1, suggesting that PARP1 SUMOylation mediated specific protein-protein interactions.
PICH Interacts with Multiple SUMOylated Substrates and SUMO Chain-The prominent 250-kDa band present only in the SUMOylated PARP1-N650 pulldown fraction (Fig. 1c, asterisk) was subjected to LC-MS/MS analysis and identified as ERCC6-like protein, also known as PICH. To confirm the LC-MS/MS result, we prepared an anti-PICH antibody with Xenopus laevis PICH fragments. Full-length Xenopus laevis PICH cDNA information is not available in databases, but we found expressed sequence tag clones that show homology to human PICH. Thus, we isolated these clones from Xenopus cDNA and then prepared proteins for antigens. Thus far, we have been unable to obtain a full-length Xenopus laevis PICH cDNA using PCR-based cloning. Immunoblotting of the pulldown fractions with a PICH-specific antibody confirmed that PICH specifically interacts with SUMOylated PARP1-N650 (Fig. 1d). To further characterize the PICH/SUMO interaction, we examined whether the interaction could be regulated by cell cycle stages as well as its specificity for SUMOylated proteins. Pulldown assays performed with mitotic and interphase extracts indicated that PICH interacted with SUMOylated PARP1-N650, irrespective of the cell cycle stage (Fig. 1e). Additionally, PICH bound to a SUMOylated TopoII␣ fragment, another major SUMOylated protein at mitotic chromosomes (Fig. 1f) (11,13). PICH also bound to SUMO chain and not to a single SUMO moiety, suggesting the presence of potentially multiple SIMs in PICH (Fig. 1g). These results suggest that PICH interacts with the SUMO moiety, regardless of the cell cycle stage and species of protein that is SUMOylated. To confirm whether the PICH/ SUMO interaction is conserved for PICH hs , we performed a pulldown assay with XEE expressing PICH hs . Consistent with endogenous PICH, PICH hs specifically bound to SUMOylated PARP1, indicating that the PICH/SUMO interaction is conserved between Xenopus and humans (Fig. 1h). Because PICH forms a complex with other proteins (30 -32), it is possible that PICH interacts with SUMOylated PARP1 through its binding partner(s). To examine whether PICH directly binds to SUMOylated proteins, we prepared a recombinant PICH hs protein by expressing it in yeast, P. pastoris. Pulldown assays were performed using PARP1-N650 and the TopoII␣ fragment as baits. As shown in Fig. 1i, recombinant PICH hs clearly bound more highly to the SUMOylated baits than to the non-SUMOylated baits, indicating that PICH could directly bind to SUMOylated substrates and, thus, could have a SIM in its primary sequence. Supporting this observation, computational prediction indicated multiple potential SIMs in the PICH hs primary sequence.

PICH Is Enriched at the Centromere of Mitotic Chromosomes, and Its Localization Is Not Dependent on SUMOylation-
Mitotic chromosomal SUMOylation is mainly detected at the centromere. We have recently observed that some centromeric proteins not only interact with SUMO-modified TopoII␣ but also require mitotic SUMOylation for their proper localization. 4 PICH was shown to localize at mitotic centromeres in mammalian cells (23,33), indicating that its localization might be dependent on SUMOylation. To test this possibility, condensed replicated chromosomes were isolated in the presence and absence of mitotic SUMOylation and were subjected to immunofluorescence analysis using an anti-PICH antibody. Endogenous PICH was enriched at the centromere of replicated mitotic chromosomes, co-localizing with CENPA, a centromere-specific H3 variant, and SUMO2/3. This enrichment was not altered when SUMOylation was inhibited by the addition of a dominant-negative form of Ubc9 (Fig. 2a). The localization of EGFP-tagged PICH hs was similar to endogenous PICH and was not altered upon the inhibition of SUMOylation (Fig. 2b). To bolster our observation that SUMOylation does not alter PICH localization on mitotic chromosomes, the amount of endogenous PICH was analyzed by immunoblotting. Mitotic chromosomes were isolated from XEE, and the amount of PICH on chromosomes with and without SUMOylation was evaluated using an anti-PICH antibody. Consistent with the immunofluorescence data, the overall amount of PICH was not altered, but surprisingly, we observed a shift above the expected After incubation with XEE and washes, the bound proteins were incubated with the catalytic domain of SENP2. SENP2 assisted in the elimination of SUMOylated PARP1/TopoII␣-CTD bands during the analysis by deconjugating SUMO. c, isolation of SUMOylated PARP1-specific binding proteins. A pulldown assay was performed with the non-SUMOylated (PARP1) and SUMOylated PARP1-N650 (S-PARP1) fragment, which were bound to S-agarose beads. Bound proteins were visualized by silver staining. SENP2 digestion eliminated the SUMOylated PARP1-N650 bands. A 250-kDa protein that specifically interacted with S-PARP1 (indicated by an asterisk) was identified as PICH by LC-MS/MS. d, specific interaction of PICH with SUMOylated PARP1-N650. Pulldown fractions digested by SENP2 as in c were subjected to immunoblotting using an affinity-purified anti-PICH antibody. The CSF input lane on the membrane was ϳ3% of the XEE used for the assay. The amount of bait (PARP1-N650) was analyzed by immunoblotting using S-tag HRP. e, cell cycle-independent PICH interaction with SUMOylated substrates. PICH interaction with SUMOylated PARP1-N650 was analyzed by pulldown assays using mitotic or interphase XEE. S-tag HRP was used to detect the amount of bait. f, PICH interacts with multiple SUMOylated substrates. Pulldown assays were performed with non-SUMOylated and the SUMOylated TopoII␣ CTD (SCTD). SENP2 eluted fractions were analyzed by immunoblotting using an anti-PICH antibody. S-tag HRP was used as the bait loading control after SENP2 digestion. g, PICH interacts with SUMO chain. A pulldown assay was performed with GFP-SUMO and GFP-SUMO chain. The pulled down fractions were analyzed by silver staining and immunoblotting using an anti-PICH antibody. h, PICH-SUMO interaction is conserved in humans. XEE expressing EGFP-tagged PICH hs was subjected to pulldown assays. PICH hs in pulldown fractions was analyzed by immunoblotting with an anti-GFP antibody. S-tag HRP depicted the amount of bait in each sample. I, PICH hs directly interacts with SUMOylated PARP1-N650 and CTD. Purified recombinant PICH hs fused to a T7 tag was incubated with non-SUMOylated and SUMOylated PARP1-N650 or TopoII␣-CTD. The bound fraction was analyzed by immunoblotting using an anti-T7 tag-HRP antibody. molecular weight of PICH. The shift was absent on the chromosomes isolated under SUMOylation inhibition by dnUbc9 (Fig. 2c). This finding suggests that PICH is most likely posttranslationally modified in a SUMO-dependent manner on mitotic chromosomes. To test whether the posttranslational modification was SUMOylation itself, we added EGFP (ϳ25 KDa)-tagged SUMO2GG to the mitotic extract and assessed supershifted PICH on mitotic chromosomes. A fraction of PICH indeed was supershifted with the addition of EGFP-SUMO2GG, similar to a previously identified SUMO substrate, PARP1 (12). This shift was not observed on chromosomes isolated from extracts with dnUbc9 (Fig. 2d). Altogether, we concluded that PICH is a SUMO substrate on mitotic chromosomes.
PICH hs Is a Strong PIASy-dependent SUMOylation Substrate in Vitro-To examine whether PICH can be SUMOylated in vitro, similar to our previously identified substrates, TopoII␣ and PARP1 (12, 14), we performed an in vitro SUMOylation assay with recombinant PICH hs as a substrate. In contrast to PARP1, PICH hs could not be SUMOylated at the highest Ubc9 concentration (600 nM) tested, at which PARP1 could be modified by SUMO2 (Fig. 3a) (12). PICH modification by SUMO2, however, was detected in the reaction with the lowest concentration of PIASy (10 nM) (Fig. 3b), a major mitotic SUMO E3 ligase. Most SUMO substrates, including TopoII␣ and PARP1, can be SUMOylated in the absence of the E3 ligase in an in vitro assay, albeit the efficiency of the modification is much lower than the reaction with E3 ligases. Within this context, PICH is a unique SUMO substrate that eminently requires an E3 ligase for its modification in vitro.
SUMOylation of PICH hs Alters Its Ability to Bind to DNA in Vitro-Because PICH is suggested to play a role in centromeric nucleosome eviction during mitosis, we sought to examine whether PICH SUMOylation has any impact on its enzymatic activity (30). Because PICH has been previously characterized as a DNA-dependent ATPase (33), we examined the effect of FIGURE 2. PICH is enriched at the centromere independently of mitotic SUMOylation and is SUMOylated on mitotic chromosomes. a, PICH is enriched at the centromere, co-localizing with CENPA and SUMO2/3, and the inhibition of SUMOylation by dnUbc9 does not alter PICH localization at the mitotic chromosomes. Replicated mitotic chromosomes were isolated from XEE and subjected to immunofluorescence staining with the indicated antibodies. Cont., control. b, PICH hs was enriched at the centromere, similar to endogenous PICH on mitotic chromosomes isolated from XEE. PICH hs -EGFP mRNA was added to XEE with and without the addition of dnUbc9 and was visualized on mitotic chromosomes along with CENPA and SUMO2/3. c, PICH is posttranslationally modified on mitotic chromosomes in XEE. Non-replicated mitotic chromosomes were isolated from XEE and analyzed by immunoblotting with an anti-PICH antibody. SUMOylation was inhibited by the addition of dnUbc9. d, PICH is SUMOylated on mitotic chromosomes in XEE. SUMO2GG-EGFP was added to the XEE, and non-replicated mitotic chromosomes were isolated and analyzed by immunoblotting. PARP1, a known SUMO substrate, served as a positive control for the PARP1-SUMO-EGFP supershift (indicated with asterisks).
PICH SUMOylation on the PICH/DNA interaction. PICH hs was SUMOylated in vitro (Fig. 3c), and its ability to bind DNA was observed by an electrophoretic mobility shift assay by adding DNA to the in vitro SUMOylation reaction. The mobility of the DNA was significantly reduced when PICH hs was SUMOylated in comparison with non-SUMOylated PICH hs (Fig. 3c). We still observed a modest shift in the DNA in the lane where PICH hs was subjected to SUMOylation (Fig. 3c); however, this result was expected because we could not obtain more than 50% SUMOylated PICH hs in the reactions (Fig. 3c). Nevertheless, the quantification of the remaining DNA with or without SUMOylation from three independent experiments indicated that the DNA/PICH hs interaction is negatively affected by more than 20% when PICH hs is SUMOylated (Fig.  3d). This result suggests that the SUMOylation of PICH might impair its DNA-dependent ATPase activity. Collectively, our findings suggest that the SUMOylation of PICH at mitotic centromeres could regulate its activity during mitosis.

DISCUSSION
Our results imply that two mechanisms of the SUMOylationdependent regulation of PICH function at the mitotic centromere: 1) the SUMOylation of PICH, which could inhibit the PICH/DNA interaction; and 2) the interaction of SUMOylated centromeric proteins (PARP1 and TopoII␣) through the SIM of PICH. This novel regulation of PICH function by mitotic SUMOylation might affect the regulation of centromeric nucleosomes by PICH, which has been shown to be important for resolving sister chromatids during anaphase.
Inhibition of PICH Activity by SUMOylation-The inhibition of DNA binding by the SUMOylation of PICH could be the result of 1) a direct effect on the DNA binding site of PICH by SUMOylation or 2) a conformational change by intramolecular interactions of SUMO and SIM. In the first case, we expect that the SUMOylation sites of PICH could be close to its DNA binding site, which is currently not defined. Because PICH SUMOylation could not be observed in the Ubc9-dependent reaction, the SUMOylation sites might not be a canonical SUMOylation sequence that can be mediated by direct interaction with the Ubc9-SUMO adduct (29,34). The identification of the SUMOylation sites and the SIM of PICH will further help us uncover these questions.
Interaction of PICH with Other SUMOylated Proteins-We initially identified PICH as one of the binding proteins of SUMOylated PARP1 in XEE, but further analyses showed that PICH also bound to SUMOylated TopoII␣ C-terminal domain (CTD) and SUMO chain (Fig. 1, d, f, and g). This finding suggests that PICH can bind non-specifically to SUMOylated proteins. The DNA helicase mutated in Bloom syndrome, BLM, a known PICH-binding protein, is SUMOylated in cells (35). Thus, it is possible that the SUMOylation of BLM could mod- ulate BLM-PICH complex function via an interaction between the SUMO moiety on BLM and the SIM of PICH. Another piece of intriguing evidence could be in relation to the SUMOylation of TopoII␣. The inhibition of TopoII␣ activity by ICRF-193 treatment has been shown to increase PICH-loaded ultrafine DNA bridges (36). Interestingly, the same inhibitor is known to increase TopoII␣ SUMOylation in mitosis (37). Thus, it is possible that the hyper-SUMOylation of TopoII␣ by  contributes to the retention of PICH at ultrafine DNA bridges during anaphase, in addition to the maintenance of catenated DNA via the inhibition of TopoII␣ activity. The possibility remains that our initial hypothesis of PICH being a potential PARP1 substrate for the PARylation of SUMOylated PARP1 is true. To date, we have not been able to detect the PARylation of PICH in an in vitro assay, although this hypothesis could hold true for mitotic chromosomes.
Implication of SUMO-dependent Regulation of PICH for Mitotic Centromere Organization-The ATPase activity of PICH is potentially required for centromeric nucleosome eviction (30); therefore, the SUMOylation of PICH could attenuate PICH-dependent centromeric nucleosome eviction by inhibiting the DNA-dependent ATPase activity of PICH. Within this context, because the inhibition of PICH SUMOylation could increase the activity of PICH at mitotic centromeres, fewer centromeric histones are expected when PICH SUMOylation is inhibited. Supporting that notion, a previous study indicated that the elimination of PICH in cells increases centromeric histones (30). Recent in vitro data with PICH did not show the efficient remodeling activity of nucleosomes with canonical histones or octamers composed of the centromeric histone variant CENPA (38), indicating an unidentified histone specificity for PICH nucleosome eviction function at centromeres. Combining PICH's capability of interacting with SUMO and considering the potential SUMOylation of histones at heterochromatin loci (39), it is possible that the nucleosome remodeling activity of PICH is more effective toward SUMOylated histones. Future studies using potential SUMOylation-deficient mutants and SUMO interaction-deficient mutants in XEE and somatic cells will provide insight into the precise role of the SUMOylation-dependent regulation by PICH of chromatin organization at mitotic centromeres.