Nuclear Calcium Signaling Induces Expression of the Synaptic Organizers Lrrtm1 and Lrrtm2*

Background: Lrrtms are synaptic organizers and can induce presynaptic differentiation. Results: Lrrtm1 and Lrrtm2 mRNA expression is controlled by synaptic activity and nuclear calcium signaling. Conclusion: The results define a pathway for the transcriptional regulation of two synaptic organizers. Significance: Nuclear calcium controls the expression of synaptogenic proteins. Calcium transients in the cell nucleus evoked by synaptic activity in hippocampal neurons function as a signaling end point in synapse-to-nucleus communication. As an important regulator of neuronal gene expression, nuclear calcium is involved in the conversion of synaptic stimuli into functional and structural changes of neurons. Here we identify two synaptic organizers, Lrrtm1 and Lrrtm2, as targets of nuclear calcium signaling. Expression of both Lrrtm1 and Lrrtm2 increased in a synaptic NMDA receptor- and nuclear calcium-dependent manner in hippocampal neurons within 2–4 h after the induction of action potential bursting. Induction of Lrrtm1 and Lrrtm2 occurred independently of the need for new protein synthesis and required calcium/calmodulin-dependent protein kinases and the nuclear calcium signaling target CREB-binding protein. Analysis of reporter gene constructs revealed a functional cAMP response element in the proximal promoter of Lrrtm2, indicating that at least Lrrtm2 is regulated by the classical nuclear Ca2+/calmodulin-dependent protein kinase IV-CREB/CREB-binding protein pathway. These results suggest that one mechanism by which nuclear calcium signaling controls neuronal network function is by regulating the expression of Lrrtm1 and Lrrtm2.

The ability of neurons to undergo structural and functional changes in response to synaptic stimulation is not only important for brain development but also forms the basis for virtually all adaptive processes in the adult nervous system, including information storage, memory formation, the buildup of neuroprotection, and the development of chronic pain (1)(2)(3)(4)(5)(6)(7)(8). Gene programs activated by synaptic stimuli are required for adaptations to be persistent (4,9). The transduction of signals from the synapse to the nucleus is primarily mediated by calcium signals (10). In particular, calcium transients in the cell nucleus function as signaling end points in synapse-to-nucleus communica-tion (11). Nuclear calcium has emerged as one of the most potent regulators of neuronal gene expression (2,11). It acts primarily via the nuclear calcium/calmodulin (CaM)-dependent 2 protein kinases II and IV to control the activity and/or localization of transcriptional regulators, including CREB, CBP, MeCP2, Fox03a, and class IIa histone deacetylases (12)(13)(14)(15)(16). Inhibition of nuclear calcium signaling compromises the ability of neurons to increase their neuroprotective activity in response to synaptic activity (2). It also blocks the conversion of memories from labile to persistent forms and attenuates the development of chronic pain (3,17). In the context of acquired neuroprotection, a nuclear calcium-regulated core gene program has been identified that mediates this process (2). The picture of how activity-driven gene transcription contributes to synaptic plasticity and cognitive functions is less complete, although several memory-relevant, nuclear calcium-regulated genes have been described, including VEGFD, Arc, Dnmt3a2, Homer1, Npas4, and nr4a1 (2, 18 -25). In recent years, synaptic cell adhesion molecules have attracted much attention as possible master regulators of synapse function and plasticity. Two of them are leucine-rich repeat transmembrane neuronal 1 and 2 (Lrrtm1 and Lrrtm2). These molecules have been identified as synaptogenic proteins (26) that contribute to the regulation of synapse density and synaptic transmission (27)(28)(29). Here we show that Lrrtm1 and Lrrtm2 are targets of nuclear calcium signaling in hippocampal neurons. NaHCO 3 , 5.3 mM KCl, 1 mM MgCl 2 , 2 mM CaCl 2 , 10 mM HEPES (pH 7.4), 1 mM glycine, 30 mM glucose, 0.5 mM sodium pyruvate, and 0.001% phenol red) (30) and minimum Eagle's medium (with Earle's salt and without L-glutamine) (Invitrogen, 9:1, vol: vol) supplemented with insulin-transferrin-sodium selenite media supplement (6.3-5.7-7.5 g/ml, Sigma), and penicillin/ streptomycin solution (1:200, Sigma) (30). Following the medium change on day in vitro 8, half of the medium was changed every second day to provide a continuous supply of growth and trophic factors.
Pharmacological Treatments, RNA Isolation, and Quantitative PCR-Pharmacological treatments were done after a culturing period of 10 -12 days in vitro during which hippocampal neurons expressed functional glutamate receptors (NMDA/ AMPA/kainate) and developed a rich network of synaptic contacts (31,32). Action potential bursting in hippocampal neurons was induced at days in vitro 10 -12 by supplementing the medium with the GABA A receptor antagonist bicuculline (50 M, Alexis) for 1-16 h (33). For the pharmacological inhibitor experiments, neurons were treated for 2-4 h with bicuculline, either with or without a 45-min pretreatment with the pharmacological inhibitors MK801 (10 M, Sigma), KN62 (5 M, Calbiochem), and anisomycin (20 g/ml, Applichem). Cells were harvested in RNeasy lysis buffer (Qiagen), and RNA was isolated using an RNeasy mini kit (Qiagen) according to the instructions of the manufacturer, with additional on-column DNase digestion during RNA purification. cDNA was synthesized from 1 g of total RNA using a high-capacity cDNA reverse transcription kit (Applied Biosystems) according to the instructions of the manufacturer.
Quantitative RT-PCR was done on an ABI7300 thermal cycler using universal quantitative PCR master mix with TaqMan gene expression assays (Applied Biosystems) for the following genes: Gusb (Mm00446953_m1), c-fos (Mm00487425_m1), Atf3 (Mm00476032_m1), Lrrtm1 (Mm00551337_g1), and Lrrtm2 (Mm00997210_g1). The expression levels of the target genes were normalized to the relative ratio of the expression of the housekeeping gene Gusb. For analyses of statistical significance, one-way analysis of variance (ANOVA) was performed, followed by Tukey post hoc analysis. The data represent mean values Ϯ S.E. from at least three independent experiments, except for the results obtained for Atf3 shown in Fig. 1D, which were log-transformed and autoscaled. Mean Ϯ SD was calculated (34). For graphical representation, the data were back-transformed to the original scale. Error bars represent upper and lower limits back-transformed as mean Ϯ S.D. Data from three independent experiments are shown.
Immunoblot signals were quantified using ImageJ software. The Lrrtm2 signals were normalized to that of tubulin. Three independent experiments were performed. Data represent mean values Ϯ S.E.
Calcium Imaging-Calcium imaging using Fluo-3 or a recombinant calcium sensor (35)(36)(37) was done with mouse hippocampal neurons plated on poly-D-lysine/laminin-coated coverslips. After a culturing period of 10 -12 days in vitro, neurons were loaded with Fluo-3 (Invitrogen) for 45 min in CO 2 -independent salt-glucose-glycine solution (SGG ind ) (140.1 mM NaCl, 5.3 mM KCl, 1 mM MgCl 2 , 2 mM CaCl 2 , 10 mM HEPES (pH 7.4), 1 mM glycine, 30 mM glucose, and 0.5 mM sodium pyruvate) (30). Cells were washed with SGG ind , and images were acquired with a Leica SP2 confocal microscope. To calibrate the fluorescence signal (F), Fluo-3 was saturated by adding 50 M ionomycin (F max ) (Sigma-Aldrich) and then quenched with MnCl 2 (F min ). [Ca 2ϩ ] was expressed as a function of the Fluo-3 fluorescence K d ϫ ((F Ϫ F min )/(F max Ϫ F)) (36). As a recombinant calcium sensor we used GCaMP6F (35), which we targeted to the cell nucleus by means of fusion to a nuclear localization signal (NLS) (GCaMP6F-NLS). 3 Neurons were infected on day in vitro 4 with a recombinant adeno-associated virus (rAAV) containing an expression cassette for GCaMP6F-NLS and were imaged on days in vitro 10 -12. The data for GCaMP6F-NLS are presented as ⌬F/F0 ϭ (F Ϫ F 0 )/F 0 (37), where F represents the average background-subtracted emission fluorescence intensity in a region of interest, and F 0 represents the baseline fluorescence measured prior to the stimulation. Immunostaining of the rAAV-GCaMP6F-NLS-infected neurons was done on days in vitro 10 -12 using an antibody to GFP (mouse monoclonal ab, Invitrogen) and Hoechst 33258 for nuclear counterstaining.
Reporter Gene Studies-Rat hippocampal neurons from newborn Sprague-Dawley rats (Charles River Laboratories) were plated on poly-D-lysine/laminin-coated culture dishes at a density of ϳ400,000 cells/1 ml Neurobasal medium containing 1% rat serum and B27 (Invitrogen). Transfection was done on day in vitro 10 with Lrrtm2 WT -Fluc and Lrrtm2 ⌬CRE -Fluc, respectively, using Lipofectamine 2000 (Invitrogen) according to the instructions of the manufacturer. pGL4.29[luc2P/CRE/Hygro] (Promega), a plasmid containing a CRE site as reporter gene-driving element, was included as a positive control. For normalization of the luciferase assays, pGL4.83[hRlucP/Puro] (Promega) containing the promoter of human EF1␣ (elongation factor-1␣, a gift from P. Pruunsild and M. Sepp (38)) was used at a ratio of 10:1 (promoter:normalizer). Transfection was performed using 1 g of plasmid DNA plus 1 l of Lipofectamine 2000 in a final volume of 250 l of Neurobasal medium. The neurons were pretreated with 2-amino-5-phosphonovaleric acid (20 M, Biotrend) overnight and stimulated on day in vitro 11 with bicuculline (50 M) and 4-aminopyridine (250 M, Sigma) for 8 h.
The luciferase assay was performed using the Dual-Glo Luciferase assay system (Promega) according to the instructions of the manufacturer. Chemiluminescence was measured by a GloMax luminometer and software (Promega and Turner Biosystems, respectively). Induction values were considered when the positive control reached Ն100-fold relative luciferase units. For presentation of the relative luciferase activity data, the background signals from untransfected neurons were subtracted from signals obtained from transfected cells. For analyses of statistical significance, Student's t tests were performed (two-sample, assuming equal variances). Data represent mean values Ϯ S.E. from four independent experiments, each performed in triplicate.
Virus Production and Infection-The procedure of rAAV production has been described previously (39). In brief, human embryonic kidney 293 cells were transfected with the rAAV expression plasmid and the adeno helper plasmids 179 and 180 for AAV capsid 1 protein expression and 181 for AAV capsid 2 protein expression by standard calcium phosphate transfection (39). 72 h after transfection, cells were harvested, and the virus was purified using HiTrap heparin columns (GE Healthcare).
Data for CaMBP4 and E1A are represented as mean values Ϯ S.E. from at least three independent experiments. For analyses of statistical significance, one-way ANOVA was performed. For post hoc comparison, Tukey or Fisher's least significant difference test were applied where appropriate.
Chromatin Immunoprecipitation-Chromatin immunoprecipitation was done using Magna ChIP chromatin immunoprecipitation kit (Millipore) according to the instructions of the manufacturer. In brief, three dishes (diameter, 6 cm) of cultured mouse hippocampal neurons, days in vitro 11-12, were treated with bicuculline (50 M) for 0.5-4 h and then fixed by addition of freshly prepared paraformaldehyde (1%). Cells were harvested and lysed in a total volume of 300 l of nuclear lysis buffer (Millipore). Chromatin was sheared to fragments of 200 -1000 bp by applying eight pulses of 5-s duration and 20% power output using a Branson digital sonifier. 50 l of sonicated DNA per immunoprecipitation were incubated with 2 g of ␣-CREB and magnetic protein A/G beads (Millipore) overnight at 4°C. Protein-DNA complexes were eluted in 100 l of elu-tion buffer (Millipore), and DNA was purified using QIAquick PCR purification kit (Qiagen). The following antibodies were used: ␣-CREB (rabbit monoclonal ab, Cell Signaling Technology) and rabbit IgG (Santa Cruz Biotechnology).
Four independent experiments were performed, and each sample was measured in triplicate with primers detecting the gene-specific regions. The amount of immunoprecipitated DNA is presented as percent of input and -fold enrichment over IgG. Statistical significance was assessed by Student's t test. The data represent mean values Ϯ S.E.

Activity-dependent Expression of Lrrtm1 and Lrrtm2-The
Lrrtm family has been identified recently as a group of proteins that can induce synaptic differentiation (26). Their mRNA levels have been shown to be regulated developmentally, with increasing expression during embryogenesis and a peak level at the day of birth that persists into adulthood (40). Because mechanisms that are important during embryonic development may function in a similar fashion in neuronal activityinduced plasticity in the adult nervous system, we investigated whether Lrrtm1 and Lrrtm2, the two most studied members of the Lrrtm family, are regulated by neuronal activity. To study their activity-induced expression, a network of cultured hippocampal neurons was exposed to the GABA A receptor antagonist bicuculline. GABAergic interneurons, which represent about 10% of the neuronal population, impose a tonic inhibition onto the neuronal network (33). Removal of GABA A ergic inhibition with bicuculline leads to action potential (AP) bursting (33), which stimulates calcium entry through synaptic NMDA receptors, induces nuclear calcium-dependent transcription, and activates a variety of gene programs (2,13,41,42). A time course analysis revealed peak expression levels of Lrrtm1 and Lrrtm2 mRNAs 4 and 2 h, respectively, after the induction of AP bursting (Fig. 1A). The induction detected using quantitative reverse transcriptase PCR was about 2-fold for both Lrrtm1 and Lrrtm2. We next aimed at analyzing Lrrtm1/2 protein expression using immunoblot analyses. Because of the lack of suitable antibodies to Lrrtm1, we focused our efforts on Lrrtm2. Of a panel of different commercially available antibodies, only one sheep polyclonal antibody was useful. Although this antibody detected several bands in the immunoblots, by using RNAi-mediated knockdown of Lrrtm2 as well as Lrrtm2 overexpression we were able to unambiguously identify the right band (Fig. 1B, left panel; the arrow indicates the Lrrtm2-specific signal). Quantitative assessment of Nuclear Calcium Regulation of Lrrtm1 and Lrrtm2 FEBRUARY 27, 2015 • VOLUME 290 • NUMBER 9 several immunoblot experiments revealed a small reproducible increase of about 25% in Lrrtm2 protein expression 8 h after the onset of AP bursting, which, however, in the statistical analysis reached a p value of only 0.25 (Fig. 1B, right panel).
Similar to c-fos and Atf3, two typical neuronal activity-and calcium-regulated immediate-early genes (2,30,43) analyzed in parallel, the induction of Lrrtm1 and Lrrtm2 mRNA by synaptic activity was dependent on NMDA receptor activation and blocked by the non-competitive NMDA receptor antagonist MK 801 (Fig. 1C). Furthermore, the regulation of Lrrtm1 and Lrrtm2 expression resembles that of classical immediate-early genes inasmuch as their induction by neuronal activity was not inhibited by the protein synthesis inhibitor anisomycin and, therefore, occurs independently of ongoing protein synthesis (Fig. 1D).
Signaling Pathways Involved in Activity-induced Expression of Lrrtm1 and Lrrtm2-We next investigated the role of CaM kinases in the activity-induced up-regulation of Lrrtm1 and Lrrtm2. We found that blockade of the CaM kinases using KN62 completely abolished the activity-induced increase in Lrrtm1 and Lrrtm2 mRNA levels ( Fig. 2A). Because there is a known inhibitory effect of KN62 on voltage-gated calcium channels that might affect the generation of AP bursting (44,45), we performed calcium imaging experiments to ensure that KN62 application did not interfere with activity-induced calcium influx into the neuron. We found that, in the hippocampal culture system, KN62, when used at a concentration of 5 M, did not compromise bicuculline-induced calcium transients ( Fig. 2B and Ref. 14). Therefore, the inhibition of the bicuculline-induced increase in Lrrtm1 and Lrrtm2 mRNA levels by KN62 reflects the requirement for CaM kinases in activity-dependent regulation of these genes.
Nuclear Calcium Signaling Regulates the Induction of Lrrtm1 and Lrrtm2-We next investigated the possible role of nuclear calcium-CaM kinase signaling in the activity-dependent regulation of Lrrtm1 and Lrrtm2. Nuclear calcium is a known activator of nuclear-localized CaM kinases such as CaMK II and CaMK IV, both of which are important factors in mediating activity-induced genomic responses (2, 14, 46 -48). To study the function of nuclear calcium signaling in the regulation of Lrrtm1 and Lrrtm2, we used CaMBP4, a nuclear protein that contains four repeats of the M13 calmodulin binding peptide from myosin light chain kinase. It binds to and inactivates the nuclear calcium-CaM complex (49). CaMBP4 has been used Treatment of the neurons with dimethyl sulfoxide, which served as the solvent for the pharmacological compounds, for 2 or 16 h or treatment of the neurons with MK801 or KN62 for 2.5 h (see Fig. 2) did not cause a significant change in the basal mRNA levels of c-fos, Lrrtm1, and Lrrtm2 (data not shown). Treatment of the neurons with anisomycin for 2.5 h led to a small increase in the basal expression levels of mRNA levels of c-fos, Lrrtm1, and Lrrtm2 (data not shown). The data in A, C, and D were obtained from at least three independent experiments with duplicate measurements and normalized to Gusb expression. Data are mean Ϯ S.E. (Atf3 in D, mean Ϯ S.D.). Differences between groups were detected using one-way ANOVA followed by Tukey post hoc test. Significance was evaluated at a probability of 5% or less. *, p Ͻ 0.05; ****, p Ͻ 0.00005.
previously to identify nuclear calcium-regulated genes that are important for neuroprotection, memory consolidation, and the development of chronic pain (2,3,18,50,51).
Primary mouse hippocampal neurons were infected with an rAAV containing an expression cassette for CaMBP4 (rAAV-CaMBP4-mCherry) or for mCherry-NLS as a control (rAAV-mCherry-NLS). We found that the AP bursting-induced increase in mRNA expression of both Lrrtm1 and Lrrtm2 observed in uninfected and control-infected cultures was abolished in cultures infected with rAAV-CaMBP4-mCherry (Fig.  3A). CaMBP4 also blocked the activity-dependent induction of c-fos and Atf3, two known nuclear calcium-regulated genes (2, 50) that we analyzed in parallel (Fig. 3A). These results indicate that nuclear calcium signaling is required for the synaptic activity-dependent regulation of Lrrtm1 and Lrrtm2.
To confirm that AP bursting does induce calcium transients that invade the cell nucleus, we carried out calcium imaging experiments using the nuclearly targeted recombinant calcium sensor GCaMP6F-NLS. We found that AP bursting triggered by bicuculline treatment gave rise to robust increases in nuclear calcium concentration (Fig. 3B). Immunocytochemical analysis revealed colocalization of GCaMP6F-NLS with the nuclear stain Hoechst 33258, confirming the nuclear localization of the calcium sensor (Fig. 3C).
Lrrtm1 and Lrrtm2 Expression and CREB-To identify putative binding sites of transcription factors that are activated downstream of nuclear calcium signaling, we performed an online database search of a 2000-bp-long upstream region of Lrrtm1 and Lrrtm2 genes using the Transcription Element Search System to recognize transcription regulatory elements. The search retrieved two CREs in the immediate vicinity of the transcription start site of Lrrtm2. Because CRE functions as a nuclear calcium response element (52), its presence in the Lrrtm2 promoter might confer nuclear calcium responsiveness to this gene. The CRE is bound by CREB, a transcription factor that, together with CBP, forms a prototypical nuclear calciumcontrolled transcription-regulating complex (12,13,53). To study the role of the CREs in the promoter of Lrrtm2, we constructed two Lrrtm2 promoter-containing reporter plasmids. The wild-type reporter (pLrrtm2 WT -FLuc) consists of a firefly luciferase (FLuc) reporter gene driven by a 356-bp-long sequence of the mouse Lrrtm2 promoter region harboring a TATA box as well as a half and a full CRE site (Fig. 4A, top  panel). Mutations were introduced into both CREs to generate a reporter construct that lacks the binding sites for CREB (pLrrtm2 ⌬CRE -FLuc) (Fig. 4A, bottom panel). These constructs were transfected into hippocampal neurons and tested for their regulation by neuronal activity. To reduce the basal level of reporter gene expression, the neurons were kept in a medium containing 20 M 2-amino-5-phosphonovaleric acid for 16 h prior to AP bursting, which was induced by bicuculline treatment in the presence of 4-aminopyridine, a weak potassium channel blocker that increases the bursting frequency (54). We found that bicuculline/4-aminopyridine treatment lead to a small but significant 1.5-fold induction of the firefly reporter signal generated by the wild-type promoter, pLrrtm2 WT -FLuc (Fig. 4B). This induction was not observed with the firefly reporter containing the mutant promoter, pLrrtm2 ⌬CRE -FLuc, which, compared with the wild type, Lrrtm2 WT -Fluc, also showed lower basal expression levels (Fig. 4B). These results indicate that the CRE present in the Lrrtm2 promoter is functionally relevant for both the basal and activity-induced regulation of Lrrtm2 expression.
We next used chromatin immunoprecipitation experiments to investigate whether CREB binds to the genomic regions adjacent to the transcription start sites of Lrrtm1 and Lrrtm2. Binding of endogenous CREB to the Lrrtm1 and Lrrtm2 regulatory regions was detected by immunoprecipitation of sheared chromatin from cultured hippocampal neurons using an antibody to CREB. The amount of immunoprecipitated DNA was measured by quantitative PCR with primers specific for the regulatory regions of Lrrtm1 (pLrrtm1) and Lrrtm2 (pLrrtm2). We observed a robust enrichment of the regulatory region of Lrrtm2 but not of Lrrtm1 (Fig. 4C). c-fos, a gene known to be regulated by CREB (55,56), was analyzed in parallel and served as a positive control (pc-fos). As expected, the promoter of c-fos was enriched in the immunoprecipitated DNA (Fig. 4C). In

Nuclear Calcium Regulation of Lrrtm1 and Lrrtm2
FEBRUARY 27, 2015 • VOLUME 290 • NUMBER 9 contrast, no significant enrichment could be measured for the promoter of Mef2c, which was used as a negative control (Fig.  4C, pMef2c). These results indicate that CREB binds to the analyzed regulatory region of Lrrtm2 but not to that of Lrrtm1.
The Role of CBP in Lrrtm1 and Lrrtm2 Expression-An online database search using the Transcription Element Search System retrieved a list of possible binding sites for transcription factors in the promoter regions of Lrrtm1 and Lrrtm2, including activator protein 1 complex (AP1), CREB, specificity protein 1 (SP1), upstream stimulatory factor, and nuclear factor of activated T cells. CBP is a transcriptional coactivator that interacts with a variety of transcription factors (57), including factors with putative binding sites in the Lrrtm1 and Lrrtm2 promoters. Also, like the expression of Lrrtm1 and Lrrtm2, nuclear calcium and CaM kinases control CBP activity (12). Therefore, we investigated the role of CBP in the regulation of Lrrtm1, Lrrtm2, and the control gene c-fos using an rAAV containing an expression cassette for the adenovirus protein E1A. E1A binds to CBP via its amino terminus-conserved region 1 (CR1) and disrupts CBP function (58,59). Mouse hippocampal neurons were infected with rAAV-E1A or with rAAV-E1A⌬CR1, a control virus expressing E1A that lacks CR1 and fails to interact with CBP (58,59). To induce AP bursting, the cultures were exposed to bicuculline, which induced an about 2-fold increase of Lrrtm1 and Lrrtm2 mRNA levels after 4 and 2 h, respectively, in uninfected cultures. This increase was reduced in neurons infected with rAAV-E1A but not in neurons infected with the control virus, rAAV-E1A⌬CR1 (Fig. 5). These results suggest a key role for CBP in the synaptic activity-dependent regulation of Lrrtm1 and Lrrtm2 expression.

DISCUSSION
This study uncovered the neuronal activity-dependent regulation of two synaptic cell adhesion molecule genes, Lrrtm1 and Lrrtm2. Both Lrrtm1 and Lrrtm2 are immediate-early genes whose synaptic activity-dependent increase in mRNA expression requires nuclear calcium signaling, the activation of CaM kinases, and CBP function.
Regulation of Genomic Responses through the Signaling Cascade of Neuronal Activity-Nuclear Calcium-CREB/CBP-Nuclear calcium-CaMKIV-CREB/CBP is a major signaling pathway in gene regulation by synaptic activity (11). Other pathways include the ERK-MAP kinase cascade, p38 MAP kinases, and calcineurin/nuclear factor of activated T-cells signaling (11). CREB is involved in many functions of the central nervous system, particularly in neuronal survival, memory formation, addiction, and neurogenesis (2, 50, 60 -63). In this study, we identified Lrrtm2 as a direct CREB target. Lrrtm2 plays a role in stabilizing AMPA receptors in the postsynaptic membrane during the maintenance of long-term potentiation (LTP) (64) and may, therefore, be part of a nuclear calcium-CREB/CBPregulated gene program required for the long-term implementation of changes in synaptic efficacy.
CBP in Lrrtm1 and Lrrtm2 Regulation-Our finding that the activity-dependent expression of both Lrrtm1 and Lrrtm2 requires CBP function may be relevant for Rubinstein-Taybi syndrome, which is caused by mutations in the CBP gene (65). It is conceivable that a disruption of the "CBP-Lrrtm axis" and possible alterations in the Lrrtm1/2-dependent maintenance of LTP (64) may contribute to the learning difficulties associated with this disease (66). Consistent with this idea is the finding that, in humans, a microdeletion affecting LRRTM2 is associated with mild cognitive impairment and developmental delay (67) that resemble the neuropsychiatric deficits found in Rubinstein-Taybi syndrome (66). Whether or not the expression levels of Lrrtm1 and Lrrtm2 are altered in Rubinstein-Taybi syndrome remains to be investigated.
Differences in Lrrtm1 and Lrrtm2 Regulation-Although both Lrrtm1 and Lrrtm2 are subject to regulation by synaptic activity, NMDA receptors, CaMK signaling, and CBP, their mRNA expression profiles follow different kinetics after stimulation, and only Lrrtm2 appears to be a direct target of CREB. This suggests the involvement of different transcription factors in the control of Lrrtm1 and Lrrtm2 expression. Their func- A and B, primary rat hippocampal neurons were transfected with wild-type (pLrrtm2 WT -FLuc) or mutant (pLrrtm2 ⌬CRE -FLuc) pGL4.10-based firefly luciferase (FLuc) reporter constructs (shown schematically in A; green letters indicate the wild-type sequence, and red letters indicate the mutant sequence) alongside an EF1␣ promoter-dependent humanized Renilla luciferase (hRLuc) construct. Following incubation with 2-amino-5-phosphonovaleric acid (20 M) overnight, the neurons were stimulated with bicuculline (50 M) plus 4-aminopyridine (250 M) for 8 h. Luciferase activities were measured and represented as -fold change in expression relative to the Lrrtm2 wild-type reporter gene construct (B). Data represent mean Ϯ S.E. from four independent experiments with triplicate measurements normalized to hRluc activities. Statistical significance was assessed by Student's t test. *, p Ͻ 0.05; n.s., not significant. C, left panel, ChIP analysis of CREB binding to the promoter of Lrrtm1 and Lrrtm2 in mouse hippocampal neurons. The binding was detected with anti-CREB antibodies in cell lysates from neurons treated with bicuculline (50 M) for 30 min. The data are represented as percent of input DNA determined by quantitative PCR using promoter-specific primers. Right panel, representation of the data as -fold enrichment of anti-CREB immunoprecipitated DNA over the IgG control for the indicated genes. The data represent mean Ϯ S.E. from four independent experiments measured in triplicates and normalized to the levels of the respective target in the input DNA. Statistical significance was assessed by Student's t test. **, p Ͻ 0.005, ***, p Ͻ 0.0005. FEBRUARY 27, 2015 • VOLUME 290 • NUMBER 9 tional properties, however, are similar and include binding to neurexin, a synaptogenic activity, and a role in LTP (26,64). Mutations and polymorphisms are found more frequently for Lrrtm1, and, in humans, are associated with handedness and schizophrenia (68,69). Only one case of LRRTM2 mutation has been reported in humans, which caused a mild cognitive impairment and developmental delay (67).

Nuclear Calcium Regulation of Lrrtm1 and Lrrtm2
Possible Function of Activity-dependent Regulation of Lrrtm1 and Lrrtm2-The functional significance of the activity-dependent regulation of Lrrtm1 and Lrrtm2 still remains to be demonstrated. Given the synaptic localization of Lrrtm1 and Lrrtm2, changes in their expression levels provide a means of returning signals and information from the nucleus back to the synapses. This process may be relevant for the maintenance of synapses. Synaptic activity induces proteolytic cleavage of postsynaptic neuroligin 1, which destabilizes the interaction with its presynaptic binding partner neurexin 1, leading to a reduction of synaptic transmission (70,71). The cleavage of neuroligin 1 is triggered by the activation of NMDA receptors and requires CaM kinase activity. The same NMDA receptor-CaM kinase signaling pathway is also responsible for the induction of Lrrtm1 and Lrrtm2 expression upon synaptic activity. Given that Lrrtm1 and Lrrtm2 can also bind neurexin, they may substitute for neuroligin 1 in the stabilization of synaptic contacts until a new neuroligin-neurexin complex is formed. Therefore, the activity-dependent regulation of Lrrtm1 and Lrrtm2 expression may help maintain the structural integrity of the synapse.