β-Apo-10′-carotenoids Modulate Placental Microsomal Triglyceride Transfer Protein Expression and Function to Optimize Transport of Intact β-Carotene to the Embryo*

β-Carotene is an important source of vitamin A for the mammalian embryo, which depends on its adequate supply to achieve proper organogenesis. In mammalian tissues, β-carotene 15,15′-oxygenase (BCO1) converts β-carotene to retinaldehyde, which is then oxidized to retinoic acid, the biologically active form of vitamin A that acts as a transcription factor ligand to regulate gene expression. β-Carotene can also be cleaved by β-carotene 9′,10′-oxygenase (BCO2) to form β-apo-10′-carotenal, a precursor of retinoic acid and a transcriptional regulator per se. The mammalian embryo obtains β-carotene from the maternal circulation. However, the molecular mechanisms that enable its transfer across the maternal-fetal barrier are not understood. Given that β-carotene is transported in the adult bloodstream by lipoproteins and that the placenta acquires, assembles, and secretes lipoproteins, we hypothesized that the aforementioned process requires placental lipoprotein biosynthesis. Here we show that β-carotene availability regulates transcription and activity of placental microsomal triglyceride transfer protein as well as expression of placental apolipoprotein B, two key players in lipoprotein biosynthesis. We also show that β-apo-10′-carotenal mediates the transcriptional regulation of microsomal triglyceride transfer protein via hepatic nuclear factor 4α and chicken ovalbumin upstream promoter transcription factor I/II. Our data provide the first in vivo evidence of the transcriptional regulatory activity of β-apocarotenoids and identify microsomal triglyceride transfer protein and its transcription factors as the targets of their action. This study demonstrates that β-carotene induces a feed-forward mechanism in the placenta to enhance the assimilation of β-carotene for proper embryogenesis.

The placenta is a critical organ that during gestation transports BC, among other nutrients, from the maternal bloodstream to the fetal circulation. Syncytiotrophoblast cells of the blood-placental barrier fulfill this transport function (18,19). Despite the importance of intact BC as a source of retinoids for the developing tissues (20 -23), the molecular mechanisms of BC transfer from mother to fetus and the factors that influence this process are largely unknown. Given that BC is a highly hydrophobic molecule transported in the adult bloodstream by lipoproteins, mainly low density lipoproteins (LDL) (24 -26), and that the placenta acquires, assembles, and secretes lipoproteins (27,28), we hypothesized that the transfer of BC from the placenta to the fetal circulation occurs via placental lipoprotein biosynthesis. The latter is predominantly regulated by apolipoprotein B (apoB), a structural component of the lipoproteins (29), and microsomal triglyceride transfer protein (MTP) that binds and chaperones lipids to the nascent apoB to prevent aberrant folding and degradation by proteasomes and assists in the intracellular assembly of apoB lipoproteins, such as chylomicrons and very low density lipoproteins (VLDL) (30,31).
Here we present evidence that the transport of intact BC from the placenta to the fetal bloodstream occurs via lipoproteins. We also show that placental lipoprotein production is regulated by BC availability. Our results identify ␤-apocarotenoids, such as apo10AL generated by BCO2, and placental MTP as critical players in this process. Specifically, they indicate that when BC is available the generation of apo10AL in placenta modulates Mttp gene expression and functions to enable transport of intact BC to the embryo.

Results
Placental Mttp Transcription and Activity Are Influenced by BC Availability-Despite the fact that the exact cellular localization of all the key regulators of lipoprotein metabolism in placenta remain elusive, it is known that placenta assembles and secretes apoB-containing lipoproteins both in vivo and in vitro (27,32). To establish whether these processes are influenced by BC availability, wild-type (WT) pregnant mice maintained during gestation on a purified diet containing 14 IU vitamin A/g of diet were intraperitoneally (i.p.) injected at 13.5 days postcoitum (dpc) with vehicle or BC (ϳ35 g of BC/g of body weight) and sacrificed 24 h later. We showed earlier that this BC administration protocol results in significant uptake of BC by the placenta without signs of embryonic toxicity (22,23,25). Dams were then sacrificed after 24 h. qPCR analysis showed that placental gene expression of Mttp was significantly increased in dams administered BC as compared with vehicleinjected dams (Fig. 1A). This transcriptional response was tissue-specific as no significant difference in Mttp mRNA levels was observed in the yolk sac, liver, or intestinal mucosa of these dams (Fig. 1A). Placental MTP protein levels were undetectable (compared with liver and yolk sac) but increased upon maternal BC administration (Fig. 1A, inset). In agreement with the increase in mRNA and protein levels, placental MTP activity FIGURE 1. Placental MTP and apoB expression. Placentas were collected from WT dams 24 h post-i.p. injection of vehicle (Veh) or BC. A, qPCR analysis of mRNA levels of Mttp in placenta (n ϭ 1-2 placentas/dam from four vehicle-treated and six BC-treated dams), yolk sac (n ϭ 1-2 yolk sac/dam from four dams for each group), liver (n ϭ 4 vehicle-treated and n ϭ 6 BC-treated dams), and intestinal mucosa (n ϭ 3 dams/treatment group). Inset, Western blot of placental protein extracts probed with a mouse anti-MTP antibody (BD Biosciences). A representative Western blot is shown. B, placental MTP activity expressed as percent transfer of lipids/mg/h (n ϭ 1-3 placentas/dam from four vehicle-treated and three BC-treated dams). C, qPCR analysis of apoB expression in placenta (n ϭ 1-2 placentas/dam from four vehicle-treated and six BC-treated dams). Data are presented as mean Ϯ S.D. (error bars) of duplicate determinations and are representative of two to three independent determinations. D, representative Western blot of placental protein extracts probed with a rabbit anti-mouse polyclonal apoB antibody (AbCam, Cambridge, UK). E, placental apoB protein levels normalized to protein loading (n ϭ 1 placenta/dam from three dams per treatment group). Normalization to albumin signal yielded similar results. Statistical analysis was by Student's t test. *, p Ͻ 0.05. Int, intestinal mucosa; Liv, liver; Plac and Pl, placenta; YS, yolk sac; S, serum.

␤-Carotene Regulation of Placental Lipoprotein Biosynthesis
was also increased in the BC-treated female mice compared with vehicle-treated controls (Fig. 1B). Furthermore, placental mRNA and protein levels of apoB were similarly increased upon BC administration ( Fig. 1, C, D, and E). Overall, these data suggest that BC may up-regulate placental lipoprotein production.
The role of MTP was further tested by daily gavage of pregnant WT mice with an MTP inhibitor, lomitapide (40 l of 1 mM stock in DMSO (33)) starting from 12.5 dpc. This inhibitor blocks synthesis and secretion of lipoproteins in all the apoB lipoprotein-producing organs (33). At 14.5 dpc, BC was injected directly into the placenta (5 l/placenta of ϳ6.5 g/l BC), and mice were sacrificed after 30 min (34). Effectiveness of lomitapide treatment was confirmed by reduced MTP activity in the liver of the dams injected with the inhibitor (Fig. 2A). Furthermore, BC transfer from placenta to embryo, assessed as the ratio between BC content in each placenta and its corresponding embryo, was severely attenuated in the dams administered lomitapide compared with controls ( Fig. 2B). Overall, these results support the hypothesis that placental MTP plays a key role in the transport of BC from the maternal circulation to the embryo.
BC Administration Influences Expression of Transcription Factors That Regulate Mttp Expression-We hypothesized that the increase in Mttp mRNA in BC-administered placenta was due to transcriptional up-regulation and quantified changes in the mRNA levels of known transcription factors that regulate Mttp expression (30). In adult tissues, 19 transcription factors bind to cis-regulatory elements less than 600 base pairs upstream of the transcriptional start site of this gene, acting as transcriptional repressors or activators (Fig. 3A) (30). To gain insights into the potential mechanism of BC-mediated placental Mttp regulation, mRNA levels of these 19 transcription factors in the placentas of WT dams administered either BC or vehicle and sacrificed after 24 h were measured by qPCR. Maternal BC administration significantly increased placental expression of the Mttp activator Hnf4␣, and slightly but significantly decreased mRNA levels of the Mttp repressors chicken ovalbumin upstream promoter transcription factor I (Coup-TFI), Coup-TFII, and Srebp1a (Fig. 3B). Of the 15 remaining transcription factors, Hnf1␣, Lrh-1, Shp, and Ppar␥ showed expression levels below the limit of detection of the qPCR analysis. FoxA2, FoxO1, Pgc1␣/␤, Srebp1c, Srebp2, Hnf1␤, Rxr␣, Ppar␣/␤, and nuclear receptor co-repressor 1 (Ncor1) did not show significant changes in mRNA levels when BC was administered to the dams (data not shown). These data indicate that increases in Hnf4␣ activator and reductions in Coup-TFI/II repressors might contribute to increased Mttp transcripts in BC-administered dams.

BC-mediated Transcriptional Response of Placental Mttp and Its Transcription Factors Is Retinoic Acid-independent-
BC is not a transcriptional regulator per se, but it serves as a precursor of transcriptional regulators, such as retinoic acid and ␤-apocarotenoids, that originate upon its enzymatic cleavage (8). Placental expression of Cyp26A1, a direct target of retinoic acid (35), was not significantly different in WT dams administered vehicle versus BC after 24 h (Fig. 3B). This finding suggests that administration of BC does not lead to significant increase in placenta retinoic acid levels at 24 h. Hence, it is likely that changes in Mttp mRNA and its transcription factors observed 24 h post-BC administration were retinoic acidindependent.
To further prove this hypothesis, retinoic acid (25 mg/kg of body weight (36,37)) was i.p. injected into WT dams at 13.5 dpc. After 24 h, the dams were sacrificed, and placental mRNA was analyzed by qPCR. As expected, Cyp26A1 expression increased 1.5-fold upon retinoic acid administration, demonstrating that our delivery method was effective (Fig. 4A). However, placental expression of Mttp and its activator Hnf4␣ were significantly decreased in dams injected with retinoic acid as compared with vehicle ( Fig. 4A). Coup-TFII and Srebp1a mRNA levels were similar in the two groups of dams, whereas Coup-TFI showed a slight decrease (p ϭ 0.05) in the presence of retinoic acid (Fig. 4A). apoB mRNA levels were also signifi-cantly decreased upon retinoic acid treatment (Fig. 4A). In agreement with the changes in mRNA, MTP activity was significantly attenuated by retinoic acid (Fig. 4B). These data indicate that retinoic acid down-regulates Mttp expression and that it is not the mediator of the BC-induced increase in MTP transcription and activity.
To rule out that retinoic acid might increase Mttp expression at an earlier time, WT dams were sacrificed 4 h after retinoic acid administration. This time point was chosen based on an experiment in which WT dams were administered BC and sacrificed at different times post-treatment. Placenta BC accumulation peaked at ϳ4 h and declined at later times in this experiment (data not shown). As expected, Cyp26A1 expression was dramatically increased (ϳ50-fold) 4 h post-retinoic acid administration (Fig. 4C). However, no difference in the expression of Mttp and its transcription factors was observed with the exception of a trend toward a decrease for Srebp1a (p ϭ 0.06) (Fig. 4D). apoB mRNA levels were slightly but significantly reduced (Fig. 4D). Thus, retinoic acid suppresses MTP transcription and activity, and another BC metabolite might be involved in its up-regulation after BC administration.
BC-dependent Transcriptional Up-regulation of Placental Mttp Is Mediated by Apo10AL-Upon asymmetric cleavage catalyzed by BCO2, BC generates apo10AL (8,9). This cleavage product can ultimately yield one molecule of retinoic acid; how- ever, it can also function itself as a transcriptional regulator by antagonizing retinoic acid actions (13)(14)(15)(16)(17). We previously showed that 24 h after a single maternal administration of BC at midgestation, Bco2 mRNA levels increased, whereas Bco1 transcription was down-regulated in the placentas of WT dams (25). Hence, we hypothesized that such transcriptional changes could result in an overall increase in ␤-apocarotenoids that could, in turn, mediate the transcriptional up-regulation of Mttp. To establish the role of apo10AL in this regulatory mechanism, Bco2 knock-out (Bco2 Ϫ/Ϫ ) dams were injected with BC or vehicle at 13.5 dpc and sacrificed after 24 h. Placental mRNA levels of Mttp, Hnf4␣, Coup-TFI, Coup-TFII, and Cyp26A1 were not different in the pregnant Bco2 Ϫ/Ϫ mice administered vehicle or BC (Fig. 5). Only Srebp1a showed a slight significant decrease (Fig. 5). These results strongly suggest that a product generated by BCO2 is involved in the BC-dependent up-regulation of Mttp.
To identify a possible mediator, WT dams were administered apo10AL (35 g/g of body weight) via i.p. injection at 13.5 dpc and sacrificed after 4 or 24 h. Apo10AL was detectable in placenta and maternal liver at both time points (Table 1), indicating that the ␤-apocarotenoid was effectively delivered to maternal and developing tissues. The placental transcriptional response to apo10AL was similar to that observed in the case of BC supplementation. Specifically, 24 h after maternal administration of apo10AL, Mttp and its activator Hnf4␣ as well as apoB mRNA levels were significantly increased, whereas expression of the repressors Coup-TFI and Coup-TFII was significantly decreased (Fig. 6A). No significant changes were observed in Srebp1a (Fig. 6A). Placental mRNA levels of Cyp26A1 were not different between the two groups of dams (Fig. 6A). In agreement with the transcriptional changes, MTP activity was also significantly enhanced in the placentas of females administered apo10AL (Fig. 6B). These results strongly support our hypothesis that apo10AL could be the mediator of the Mttp transcriptional activation induced by BC. Four hours after the administration of apo10AL, the transcription of the above mentioned genes (Fig. 6C) as well as MTP activity (Fig.  6D) did not change. Interestingly, placental transcription of the retinoic acid target gene Cyp26A1 significantly increased 4 h after maternal administration of apo10AL (Fig. 6C), suggesting that, in placenta, ␤-apocarotenoids may initially be converted into retinoic acid likely via BCO1 (to retinal first followed by oxidation to retinoic acid) (9,11). Overall, Mttp showed a late transcriptional regulation by this ␤-apocarotenoid, strongly supporting the possibility that Mttp is not a direct target of apo10AL action.
␤-Apo-10Ј-carotenol Also Increases MTP Transcription and Activity-Although it is not known which enzyme(s) could catalyze the conversion of apo10AL to ␤-apo-10Ј-carotenol (apo10OL), the latter was detected in tissues of mice fed BCcontaining diets (9). Thus, to gain some insights into whether the aldehyde or the alcohol form of the ␤-apo-10Ј-carotenoid may be the active compound responsible for regulating placental MTP function, we administered apo10OL (35 g/g of body weight) via i.p. injection to WT dams at 13.5 dpc and sacrificed them after 24 h. Detection of apo10OL in the maternal tissues (serum, 568.0 g/dl, n ϭ 2; liver, 20.4 Ϯ 8.6 g/g, n ϭ 3) confirmed effective delivery of the compound. Apo10OL enhanced placental Mttp, apoB, and Hnf4␣ transcription even more dramatically than did apo10AL (Fig. 6E). Accordingly, MTP activity was also increased (Fig. 6F), suggesting that both ␤-apo-10Јcarotenoid forms (apo10AL and apo10OL) could mediate the BC-induced regulation of MTP function.

Discussion
BC circulating in the maternal bloodstream is an important source of vitamin A for the developing mammalian embryo (20 -23). Nevertheless, the mechanisms that enable and regulate maternal-fetal transfer of the provitamin A carotenoids are still unknown despite the knowledge that BC is transported in the circulation in association with lipoprotein particles (24 -26). Our studies show that BC transport from placenta to the fetal circulation is a highly regulated process that involves lipoprotein assembly and secretion. We show that BC availability in the circulation of WT pregnant female mice increases placental transcription and activity of MTP. MTP protein levels were also increased even though they were overall very low. Placental subcellular localization of MTP remains elusive, but it is possible that the protein is expressed in the syncytiotrophoblast layer where other key molecular players of lipoprotein biosynthesis have been localized (38,39). Such restricted localization in a specific cell population relative to the whole organ could account for the overall low levels of MTP protein detected by Western blotting. An inhibitor of MTP also severely attenuates

TABLE 1 Serum, liver, and placenta concentrations of apo10AL in pregnant WT dams sacrificed 4 or 24 h post-i.p. injection of apo10AL
Analysis was performed by reverse phase HPLC. n, number of dams/group. For placenta, at 4 h, one to two placentas/dam (three dams total), n ϭ 4 placentas analyzed; at 24 h, one to two placentas/dam (two dams total), n ϭ 3 placentas analyzed. Data are presented as mean Ϯ S.D. ND, not-detectable (below detection limits). BC placental-fetal transfer in vivo. Remarkably, this effect of BC is tissue-specific as it was observed only in placenta and not in other organs known to synthesize and secrete apoB-containing lipoproteins. The reason for this tissue specificity is currently unclear. In adult tissues, Mttp expression is dependent on a conserved minimal 204-bp sequence of the promoter that contains several cis-elements (40,41). Three critical elements are HNF1, HNF4, and direct repeat 1 (DR-1). The first two ele- ments bind HNF1 and HNF4 family members that mainly function as transcriptional activators of Mttp (40,41). DR-1 could bind to HNF4 and/or other transcription factors (including COUP-TFs), mostly RXR heterodimers (2). DR-1 is critical for suppression of Mttp expression. COUP-TFI binds to the DR-1 element and abolishes the synergistic activation of the Mttp promoter by HNF1 and HNF4␣ by recruiting the nuclear receptor co-repressor 1 (NCOR1) (40,41). We surveyed the placental expression of the 19 transcription factors that bind to the ϳ600 bp upstream of the start site of Mttp. We found that Mttp activator Hnf4␣ was up-regulated, whereas its repressors Coup-TFI/II and Srepb1a were down-regulated by BC availability. Thus, changes in the binding of these transcription factors to different elements in the promoter might be involved in the regulation of MTP by BC. Despite the fact that BC is not a transcriptional regulator, it can serve as a precursor of potent transcriptional regulators, such as retinoic acid and ␤-apocarotenoids, that originate upon its enzymatic cleavage (8). Given that the expression of Cyp26A1, a direct target of retinoic acid (35), did not change upon maternal BC administration and that maternal administration of retinoic acid did not reproduce the changes in mRNA levels observed in Mttp and its transcription factors when BC was available, we inferred that the transcriptional effect of BC on Mttp is retinoic acid-independent. Moreover, the lack of Mttp transcriptional response in the placenta of Bco2 Ϫ/Ϫ dams supplemented with BC and the similar placental transcriptional response to apo10AL and BC in WT dams strongly suggest that ␤-apocarotenoids are among the active compounds that regulate placental lipoprotein biosynthesis. These findings agree with the current notion that ␤-apocarotenoids can function as transcriptional regulators. Eroglu et al. (13) showed that ␤-apocarotenoids of various chain lengths and forms (aldehyde, acid, and ketone) antagonize all-trans-retinoic acid-induced transactivation of all three retinoic acid receptor isoforms at nanomolar concentrations likely by directly competing with retinoic acid for receptor binding or by modulating the RXR oligomeric state as in the case of ␤-apo-13-carotenone (14). ␤-Apocarotenoids also inhibited the all-trans-retinoic acid-induced expression of retinoid-responsive genes in HepG2 cells (13) and antagonized the activation of RXR␣ by 9-cis-retinoic acid (15). Also, ␤-apo-14Ј-carotenal effectively inhibited agonist-induced RXR␣, PPAR␣, and PPAR␥ activation, decreasing adipogenesis (16). These cell culture-based experiments demonstrated that specific ␤-apocarotenoids function as nuclear receptor antagonists exerting an anti-vitamin A (retinoic acid) activity (17). Not only apo10AL (42) but also apo10OL (9) was detected in tissues of mice fed BC-containing diets even though the enzymes that could catalyze the conversion of ␤-apocarotenal, the primary asymmetric cleavage product of BC, into ␤-apocarotenol have not been identified. Our data show that apo10AL and apo10OL can both up-regulate MTP transcription and activity as well as the expression of its transcription factors. Although the transcriptional response induced by apo10OL seems more robust, the increase in enzymatic activity is comparable with that of the aldehyde form. Thus, we cannot unequivocally establish which of these ␤-apocarotenoids mediates the changes in gene expression or whether they need to be further metabolized to become transcriptionally active. Nevertheless, our data provide the first in vivo evidence of the transcriptional regulatory activity of these BC derivatives and identify the first in vivo targets of their action, i.e. MTP and its transcription factors.
Based on all our data, we propose that intact BC taken up by the placenta from the maternal bloodstream is transported toward the fetal circulation in association with lipoproteins likely assembled within the placenta syncytiotrophoblast cells (18,19). Placenta production of apoB-containing lipoproteins is enhanced by BC availability through a mechanism that seems to be mediated by ␤-apocarotenoids. Specifically, our data suggest that when BC is available placental Bco2 transcription (and likely activity) increases, thus enhancing the generation of apo10AL/OL from BC asymmetric cleavage. This metabolite then, directly or indirectly, increases the transcription of Hnf4␣ and represses Coup-TFI and Coup-TFII, which in turn increase the transcription of Mttp and consequently its activity, ultimately stimulating biosynthesis and transfer of BC-containing lipoproteins toward the fetus (Fig. 7A). It is relevant that apoB, whose mRNA levels are also elevated by BC and apo10AL/OL supplementation, is a target of HNF4␣ transcription action (43,44). Thus, we favor the possibility that placental apoB could also be a target of the BC/␤-apocarotenoid regulatory mechanism contributing to modulating placenta lipoprotein assembly and secretion when BC is available.
As discussed previously, ␤-apocarotenoids function by antagonizing retinoic acid activity (13)(14)(15)(16)(17), therefore implying the existence of a retinoic acid-responsive element (RARE) in the promoter region of their target gene(s). Given that Mttp and its transcription factors are affected by the maternal administration of BC or apo10AL only after 24 h, we favor the hypothesis that the effect of the provitamin A carotenoid is not directly on the promoter of Mttp even though the latter contains a putative RARE DR-1 element (40,41). Therefore, we propose that suppression of Coup-TFs and activation of Hnf4␣ contribute to increased expression of Mttp mediated by BC (Fig. 7B).
The human Hnf4␣ gene has been shown to be regulated by retinoic acid (45). Whether the promoter or any other regula-  AUGUST 26, 2016 • VOLUME 291 • NUMBER 35 tory elements of the mouse Hnf4␣ contain a functional RARE cis-element(s) responsible for its repression has not been demonstrated. However, we showed that, as in the case of the human gene (45), retinoic acid down-regulates the expression of Hnf4␣ in mouse placenta. Our data do not unequivocally demonstrate whether retinoic acid regulates Hnf4␣ directly or indirectly. Unlike Cyp26A1 (35), a known retinoic acid direct target gene, we showed that Hnf4␣ is repressed by retinoic acid in vivo only 24 h after retinoid administration, thus suggesting an indirect regulation. However, genes can also respond late to a direct regulation by retinoic acid (via a RARE promoter element) (46) as the relative potency and specificity of the RAREs depend on both the configuration and nucleotide sequence of the repeats (47). This question will need to be addressed in future studies. Whether directly on the promoter of Hnf4␣ or indirectly on the promoter of another transcription factor upstream of Hnf4␣, we hypothesize that apo10AL/OL antagonizes retinoic acid for the binding to retinoic acid receptors and/or RXRs on a RARE cis-element, ultimately turning on Hnf4␣ transcription. A similar reasoning could also be applied to Coup-TFI and Coup-TFII. Because the expression of these orphan receptors is regulated by retinoic acid treatment (48), it is expected (and confirmed by our data) that, in placenta, apo10AL/OL reduces the expression of these Mttp repressors and thus increases Mttp transcription.

␤-Carotene Regulation of Placental Lipoprotein Biosynthesis
Members of the Srebp1 family of transcription factors have also been shown to be induced by retinoic acid in few experimental systems (49,50). Whereas the transcriptional downregulation of the Mttp repressor Srebp1a in placenta of WT administered BC may be in agreement with our model, the lack of Srebp1a changes upon maternal supplementation with apo10AL/OL and its persistent down-regulation in the placenta of Bco2 Ϫ/Ϫ dams treated with BC are inconsistent, questioning the role of SREBP1a in the regulatory mechanism proposed here.
The critical role of retinoic acid in embryogenesis is well established (1). Retinoic acid is preferentially synthesized in the developing embryo from retinoid precursors rather than transferred to the embryo as such due to its spatially and temporally restricted action (6). Indeed, maternal retinoic acid administration can rescue several but not all the developmental defects in mouse mutants for retinoic acid biosynthetic enzymes, such as RALDH2, RALDH3, and RDH10 (36). Also, retinoic acid supplementation never resulted in normal live embryos at term in these strains (37), hence the notions that the placenta favors the transfer of retinol (retinoic acid precursor) versus retinoic acid (51) and that BC could be used as a precursor of retinol in placenta (52). Contrary to this hypothesis, our data suggest that the placenta is equipped with highly regulated molecular machineries that favor the transfer of intact BC (retinoic acid precursor) versus retinoic acid under a normal maternal vitamin A status. We speculate that this mechanism might provide an advantage in acquiring intact BC, a critical "safe" source of retinoid for the developing embryo, from the maternal circulation.
We cannot exclude that the increase in MTP activity caused by the maternal BC or apo10AL/OL administration could also facilitate retinyl ester transfer to the developing fetus via placental lipoproteins. Previous data from our laboratory failed to detect changes in embryonic retinol and retinyl ester levels upon a single maternal supplementation with BC (25). However, in our experimental system, the baseline concentration of retinoids in the embryo is much higher than ␤-carotene (which is indeed undetectable prior to the supplementation). Thus, it is possible that a potentially enhanced (but quantitatively small) transfer of retinoids would not be measurable over a relatively high baseline level of retinoids. Further experiments will need to be conducted under different experimental conditions to address this issue. In summary, we showed that BC regulates placental lipoprotein biosynthesis via apo10AL generated by BCO2 and that the targets of this BC-mediated transcriptional regulation are Mttp and its transcription factors Hnf4␣ and Coup-TFI/II.

Experimental Procedures
Mice and Nutritional Manipulation-WT and Bco2 Ϫ/Ϫ female mice (a generous gift from Dr. J. Von Lintig, Case Western Reserve (12)) on a mixed genetic background (C57BL/6 ϫ Sv129) were maintained on a regular chow rodent diet (Prolab Isopro RMH3000 5p75; energy from protein, fat, and carbohydrates, 26, 14, and 60%, respectively; 18 IU of vitamin A (as retinyl palmitate)/g of diet; BC, from trace to 1.2 ppm/g of diet) manufactured by LabDiet (W. F. Fisher and Son, Inc.). At 3 months of age, females were mated with males of the same genotype. At the time of vaginal plug detection, designated as 0.5 dpc, females were placed on a purified diet containing 14 IU of vitamin A/g of diet provided as retinyl palmitate. The nutritional composition of the purified diet was similar to that of the regular chow diet described above with the exception of the slightly lower vitamin A concentration (14 IU of vitamin A (as retinyl palmitate)/g of diet). Neither BC nor its metabolites were present in the purified diet (Research Diet).
All mice were maintained on a 12:12-h light/dark cycle with the period of darkness between the hours of 7:00 p.m. and 7:00 a.m. Dams were continually fed until the end of the experiment with the purified diet and water available ad libitum. All experiments were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals (53) and were reviewed and approved by the Rutgers University Animal Care and Use Committee.
MTP Activity Assay-This procedure has been published previously (54,55). In brief, small pieces (0.1 g) of liver and placenta were homogenized in low salt buffer (1 mM Tris-HCl, pH 7.6, 1 mM EGTA, and 1 mM MgCl 2 ) and centrifuged, and supernatants were used for protein determination (by Bradford assay), and triglyceride transfer activity of MTP was assayed using a commercial kit (Chylos, Inc.).
Western Blotting Analysis-Placental content of apoB and MTP was analyzed by Western blotting. For apoB, 80 g of placental protein was run on a 4 -12% gradient SDS-polyacrylamide gel. A rabbit anti-mouse polyclonal apoB antibody (AbCam, Cambridge, UK) was used for immunodetection (1:3000 dilution). For MTP, 80 g of placental protein and 1 g of liver or yolk sac extract were run on a 4 -12% gradient SDSpolyacrylamide gel. A purified mouse MTP antibody (BD Biosciences) was used for immunodetection (1:3000 dilution). Goat anti-rabbit or goat anti-mouse secondary antibodies were used in a 1:5000 dilution. Signals were normalized to albumin, detected by the apoB antibody, and to protein loading (assessed by staining membranes using Coomassie Blue). The signals were imaged using a Bio-Rad Chemidoc XRS Molecular Imager system. Expected molecular masses were as follows: apoB100, 510 kDa; apoB48, 250 kDa; MTP, 97 kDa; albumin, 65 kDa. The quantification of the membranes was completed by densitometry analysis with Quantity One software (Bio-Rad).
Statistical Analysis-Normality of the data was established using the Shapiro-Wilk test. When data were normally distributed, statistical analysis was performed by Students' t test. For data that were not normally distributed, comparisons among groups were made using the Kruskal-Wallis test followed by Mann-Whitney test. Analyses were performed with SPSS statistical software (IBM SPSS Statistics, version 19). A p value Յ0.05 was considered significant.