The Histamine H3 Receptor Differentially Modulates Mitogen-activated Protein Kinase (MAPK) and Akt Signaling in Striatonigral and Striatopallidal Neurons*

The basal ganglia have a central role in motor patterning, habits, motivated behaviors, and cognition as well as in numerous neuropsychiatric disorders. Receptors for histamine, especially the H3 receptor (H3R), are highly expressed in the striatum, the primary input nucleus of the basal ganglia, but their effects on this circuitry have been little explored. H3R interacts with dopamine (DA) receptors ex vivo; the nature and functional importance of these interactions in vivo remain obscure. We found H3R activation with the agonist R-(-)-α-methylhistamine to produce a unique time- and cell type-dependent profile of molecular signaling events in the striatum. H3 agonist treatment did not detectably alter extracellular DA levels or signaling through the cAMP/DARPP-32 signaling pathway in either D1- or D2-expressing striatal medium spiny neurons (MSNs). In D1-MSNs, H3 agonist treatment transiently activated MAPK signaling and phosphorylation of rpS6 and led to phosphorylation of GSK3β-Ser9, a novel effect. Consequences of H3 activation in D2-MSNs were completely different. MAPK signaling was unchanged, and GSK3β-Ser9 phosphorylation was reduced. At the behavioral level, two H3 agonists had no significant effect on locomotion or stereotypy, but they dramatically attenuated the locomotor activation produced by the D1 agonist SKF82958. H3 agonist co-administration blocked the activation of MAPK signaling and the phosphorylation of rpS6 produced by D1 activation in D1-MSNs, paralleling behavioral effects. In contrast, GSK3β-Ser9 phosphorylation was seen only after H3 agonist treatment, with no interactive effects. H3R signaling has been neglected in models of basal ganglia function and has implications for a range of pathophysiologies.

The basal ganglia play a critical role in the regulation of cognition, motor control, motivated behavior, and habit learning as well as in the pathophysiology of a range of neuropsychiatric conditions, including Parkinson's disease, Tourette syndrome, and drug addiction (1)(2)(3)(4)(5)(6). Factors that modulate information flow through the basal ganglia circuitry are therefore of broad importance. The roles of dopamine (DA) 4 and acetylcholine in the regulation of striatal information processing have been extensively studied. Histamine (HA) is produced in the tuberomamillary nucleus of the posterior hypothalamus; these neurons project broadly throughout the brain, including to the striatum (7). HA receptors are prominent throughout the basal ganglia (7,8), but their function in modulating this circuitry is not well understood, and very few studies have examined their functional importance in vivo (9).
The striatum (equivalent to the caudate and putamen in primates) is the largest nucleus of the basal ganglia and the primary site of cortical and thalamic input. Its principal cells, the medium spiny neurons (MSNs), can be divided into two subpopulations based on their projections. Striatonigral or "direct pathway" neurons preferentially express the D1 DA receptor and have a net disinhibitory effect on the thalamus, which processes most output from the basal ganglia. Striatopallidal or "indirect pathway" neurons preferentially express the D2 DA receptor and have a net inhibitory effect on the thalamus (3,6,10). Modulatory DA critically regulates plasticity and relative activity in D1-and D2-expressing MSNs.
The molecular processes regulated by DA in D1-and D2-expressing MSNs have been a focus of intense research (11,12). The importance of striatal regulation by DA is highlighted by the striking consequences of DA receptor stimulation, which produces locomotor activation and stereotypy (13). In D1-MSNs, D1 receptor activation leads to increased cAMP, phosphorylation of DARPP-32, and activation of the MAPK signaling pathway. In D2-MSNs, in contrast, D2 receptor activation leads to decreased cAMP and reduced phosphorylation of DARPP-32 (14) as well as ␤-arrestin-dependent dephosphorylation, and thus deactivation, of the kinase Akt, and consequent reduced phosphorylation, and thus activation, of GSK3␤ (15).
Recent clinical data have focused new attention on modulation of the basal ganglia by HA (9). A dominant-acting loss-of-function mutation in the HA biosynthetic enzyme histidine decarboxylase (Hdc) has been implicated as a rare cause of Tourette syndrome (16); other genetic analyses have suggested abnormalities in histaminergic signaling in Tourette syndrome more broadly (17,18). HDC protein is particularly concentrated in the striatum, suggesting an important role for HA there (19). We have shown loss of Hdc to lead to elevated striatal DA, increased tic-like stereotypies, and alterations in MAPK and Akt signaling (1,20,21).
HA binds to four G protein-coupled receptors, H1R-H4R (7). Of these, H3R is expressed only in the nervous system; it is highly enriched in the basal ganglia (7,8). H3R has a well established role as a presynaptic inhibitor of neurotransmitter release on both histaminergic and other neurons (7,22). In particular, it has been shown to reduce DA release in some brain regions (23). However, this may not be its primary role in the striatum (22). A series of ex vivo studies have suggested that postsynaptic H3R in MSNs forms physical complexes with both D1 and D2 receptors that modulate the signaling properties of both (24 -27). In striatal slices, H3R complexed with D1R activates MAPK signaling (25)(26)(27). Effects on Akt signaling in the striatum are less clear. H3R activation can inhibit the Akt1 target GSK3␤ in immortalized cells and cultured cortical neurons (28,29), but as these systems are not known to recapitulate the unique ␤-arrestin-dependent mechanism by which Akt and GSK3␤ are regulated in D2-MSNs, it is unclear whether this regulation will generalize to the striatum in vivo.
We characterized the regulation of striatal signaling by H3R in vivo and examined functional interactions between H3R and D1R activation. We find a unique temporal and cell-specific profile of H3 effects on MAPK and GSK3␤ signaling and striking nonlinear interactions between H3 and D1 effects at both molecular and behavioral levels. These interactions are likely to be an important and underappreciated regulator of basal ganglia function in vivo.

Results
H3R Agonist Treatment Does Not Regulate the cAMP/PKA Pathway in Striatal MSNs-Activation of the H3R has been linked to numerous molecular events that we seek to better characterize in vivo. H3R is coupled to G␣ i and thereby can reduce cAMP-mediated signaling, at least in some cells (7,30,31). This could oppose the effects of D1R activation on the cAMP/PKA/DARPP-32 signaling pathway. We therefore first sought to characterize whether H3R activation regulates cAMP-mediated signaling in the striatum in vivo. We used phosphorylation of DARPP-32, which is responsive to changes in cAMP levels in striatal MSNs, as a surrogate marker for changes in cAMP.
We characterized the effects of RAMH (45 mg/kg), an H3 agonist, on DARPP-32 phosphorylation using transgenic mice that express FLAG-and Myc-taggd DARPP-32 in D1-and D2-MSNs, respectively, permitting DARPP-32 phosphorylation to be disociably quantified in these two cell types (14). Increased cAMP and PKA activity lead to increased phosphorylation of DARPP-32 at Thr 34 (11). PKA-dependent activation of protein phosphatase 2A (PP-2A) leads to dephosphorylation of DARPP-32 at Thr 75 (32,33). However, RAMH did not pro-duce significant changes in these phosphorylation sites in either D1-or D2-MSNs (Fig. 1, A and B). Thus, H3 activation does not appear to significantly regulate the cAMP/PKA signaling pathway in either D1-or D2-MSNs in vivo, at least at this time point.
Activation of H3Rs on DA terminals has been shown ex vivo to regulate DA release (23); this represents an alternative way in which the H3R might modulate striatal function. Whether this regulation occurs in the striatum in vivo has not been established. We measured striatal DA before and after systemic administration of RAMH (45 mg/kg) using in vivo microdialysis. There was no detectable change in striatal DA levels after RAMH treatment (Fig. 1C).
Thus, these two well established mechanisms of H3R signaling appear not to be operative in vivo in the striatum under these conditions. H3R activation does not significantly regulate either extracellular DA levels or cAMP-mediated signaling. A and B, the transgenic reporter DARPP-32 was specifically immunoprecipitated from either D1-or D2-MSNs, and phosphorylation of Thr 34 and Thr 75 was analyzed by immunoblotting as described previously (14) (26). We examined the effect of RAMH on MAPK activation in vivo using immunoblotting of striatal extracts collected after systemic RAMH challenge. Phosphorylation of ERK1/2 MAPK at Thr 202 /Tyr 204 was strongly increased 15 min after RAMH administration but returned to normal by 45 min (Fig. 2A).
We next examined the cell specificity of the RAMH effect on MAPK activation using D2-EGFP transgenic mice. We found immunohistochemistry for P-MAPK to produce inadequately specific cell body signal to permit categorization and counting of positive cells. We therefore used phosphorylation of the ERK target mitogen-and stress-activated kinase 1 (MSK1) at Thr 581 as a marker of MAPK signaling (Fig. 2B) (34). (MSK1 is a target of both the ERK1/2 and p38 MAPK signaling pathways (35)). P-MSK1-positive cells were visualized in a single confocal image from the central dorsal striatum in each slice (Fig. 2C) and categorized as either co-localizing or not with GFP. In GFPnegative striatal neurons, the vast majority of which are expected to be D1-MSNs, P-MSK-Thr 581 was strongly but transiently modulated by RAMH, with immunopositive cells increasing nearly 6-fold at 15 min and returning to baseline at 45 min, paralleling the effects on MAPK seen by immunoblotting (Fig. 2D). In contrast, in GFP-positive cells, the vast major-ity of which are D2-MSNs, there was no modulation of MSK1 by RAMH (Fig. 2E).
We next examined phosphorylation of ribosomal protein S6 (rpS6). rpS6 is a key regulator of inducible translation in neurons (36). Its phosphorylation at Ser 235 /Ser 236 is regulated by numerous signaling pathways (we have shown it to depend on MAPK signaling in these neurons under some circumstances (20)), but it is also regulated by PKA, S6 kinase, and other pathways (36). A nearby pair of phosphorylation sites, Ser 240 /Ser 244 , is regulated by S6 kinase but not by MAPK signaling (36).
Immunoblotting revealed an increase in phosphorylation of rpS6 at the Ser 235 /Ser 236 site 15 min after RAMH treatment; this returned to baseline by 45 min, paralleling ERK 1/2 phosphorylation (Fig. 3A, compare with Fig. 2A). We again discriminated between D1-and D2-MSN contributions to this effect using immunostaining in D2-GFP transgenic mice. In D1-MSNs, RAMH treatment modulated Ser(P) 235 /Ser(P) 236 - rpS6 (Fig. 3B), with an increase at 15 min that returned nearly to baseline by 45 min, paralleling MSK1 activation. In D2-MSNs, there was no statistically significant effect of RAMH on Ser(P) 235 /Ser(P) 236 -rpS6, although there was nominally increased phosphorylation at 45 min that did not reach statistical significance. There was no effect of RAMH treatment on Ser(P) 240 /Ser(P) 244 -rpS6 in either MSN subtype at either time point (Fig. 3C). H3 Regulation of Akt-GSK in D1-and D2-MSNs-Akt-GSK3 signaling is regulated by D2 activation in D2-MSNs (15); its regulation by the H3 receptor in MSNs has not been investigated previously. We quantified Akt phosphorylation at Thr 308 by immunoblotting of striatal extracts (20). This revealed a significant modulation of Thr(P) 308 Akt in vivo (Fig. 4A); there was little change at 15 min but a marked reduction 45 min after RAMH injection. There was no modulation of the other regulatory site, Ser 473 . We also examined the Akt target GSK3␤-Ser 9 and found that it, too, was modulated by RAMH treatment (Fig.   4B). In the case of GSK3␤, Ser 9 phosphorylation was increased at 15 min but decreased at 45 min, indicating complex temporal regulation.
To examine the cell specificity of these effects, we again turned to immunostaining in D2-GFP transgenic mice. We focused on GSK3␤ (Fig. 4C), as anti-P-Akt antibodies showed a high background on tissue in our hands. In D1-MSNs, there was a clear but transient effect of RAMH on GSK3␤, with increased Ser 9 phosphorylation at 15 min that returned to baseline at 45 min (Fig. 4D). In D2-MSNs, in contrast, there was an equally clear but slower and qualitatively opposite modulation of Ser 9 phosphorylation, with decreasing Ser(P) 9 over time that became significant at 45 min. This dissociation explains the biphasic pattern of GSK3␤ phosphorylation seen by immunoblotting (Fig. 4B).
H3R Activation Attenuates D1 Agonist-induced Locomotor Activation-H3 activation has been reported to attenuate the locomotor activation seen after D1 stimulation in reserpinized mice (24); whether this interaction is present in intact animals has not been demonstrated. We tested the effects of the H3 agonist RAMH (45 mg/kg) and the D1 agonist SKF82958 (5 mg/kg), alone and in combination, on locomotor activity and stereotypy. RAMH injection had no effect on locomotion. SKF82958 challenge, on the other hand, produced locomotor activation as expected (37). Strikingly, the locomotor effect of D1 activation was completely eliminated by RAMH co-administration. (Fig. 5A).
Locomotion after psychostimulant treatment can be reduced by an increase in stereotypy. This is seen, for example, at high psychostimulant doses or after repeated injections (1,38). Specific D1 activation with agents such as SKF82958 does not typically produce high levels of stereotypy, but it is possible that simultaneous RAMH administration might potentiate stereotypy and reduce locomotion by this mechanism.
To examine this, we tested behavioral stereotypy after challenge with the two drugs alone and in combination. SKF82958 (5 mg/kg) produced a modest elevation in stereotypy that was not enhanced by RAMH co-administration (45 mg/kg, Fig. 5B).
We repeated the experiment with another H3 agonist, immepip (39). Administration of SKF82958 and immepip (20 mg/kg) produced the same pattern of results as RAMH. Immepip had no effect on locomotion by itself, but it blocked the increase in locomotion seen after SKF82958 (Fig. 5C).
D1-H3 Interaction in the Regulation of MAPK Signaling-We tested whether H3 and D1 agonists also interact at the level of signaling in D1-and D2-MSNs. D1 and H3 agonists have been shown previously ex vivo to interact in the regulation of MAPK in D1-MSNs (25,26). We replicated this interactive effect in vivo. Both RAMH and SKF82958 treatment increased phosphorylation of MSK (at 15 min, as shown in Fig. 3), but co-administration of both agents completely abolished this effect (Fig. 6A). There were no effects of either agent in D2-MSNs.
H3 and D1 agonists also interacted in the regulation of rpS6. Both RAMH and SKF82958 led to increased phosphorylation of rpS6, but SKF82958-induced phosphorylation was significantly greater (at least at these doses). Co-administration reduced phosphorylation back to the level of RAMH alone but not (as in the case of MSK) all the way to baseline (Fig. 6B). Again, there were no effects in D2-MSNs.
D1 Stimulation Does Not Regulate Akt-We administered RAMH (45 mg/kg) and SKF82958 (5 mg/kg) alone and in combination and examined GSK3␤-Ser 9 phosphorylation 15 min later in D1-and D2-MSNs. As before, RAMH led to increased GSK3␤ phosphorylation. In this case, however, there was no interactive effect. SKF treatment did not influence GSK3␤-Ser 9 phosphorylation either alone or in conjunction with RAMH (Fig. 6C). There were no detectable effects in D2-MSNs (note that these samples were collected at 15 min; the effects on GSK3␤ in D2-MSNs documented in Fig. 5 were not prominent until 45 min).

Discussion
HA receptors are prominent in the striatum, but how HA modulates the basal ganglia circuitry is not well understood (9). We found treatment with H3 histamine receptor agonists to attenuate the locomotor effects of the D1 agonist SKF82958. The H3 agonist RAMH modulates signaling in D1-and D2-MSNs in distinct and even opposite ways. In D1-MSNs, H3 activation transiently activates MAPK signaling, recapitulating a prominent effect of D1 activation, but it does not detectably affect cAMP/DARPP-32 signaling, and it triggers a previously uncharacterized transient phosphorylation (and thus presumptive deactivation) of GSK3␤. In D2-MSNs, H3 activation has no effect on MAPK or cAMP/DARPP-32, but it regulates Akt-GSK3␤, producing a more slowly developing dephosphorylation (and thus presumptive activation) of GSK3␤: qualitatively, the opposite of its effect in D1-MSNs. This unique profile of cellular effects suggests an important and largely unappreciated role for HA in the regulation of striatal function.   (7). G␣ i -mediated inhibition of adenylyl cyclase may explain the inhibition of transmitter release by presynaptic H3 homoreceptors and heteroreceptors (7,23,40). However, G␣ i -mediated signaling mechanisms do not explain the signaling in MSNs that we see in vivo. We find no significant modulation of cAMP/DARPP-32 signaling in either D1-or D2-MSNs.
Recent ex vivo data suggest that H3Rs heterodimerize with D1 and D2 dopamine receptors and thereby acquire unique signaling characteristics in MSNs (24 -26). Specifically, activation of H3-D1 heterodimers has been shown to activate MAPK signaling (25,26), which is consistent with the transient effect we see in D1-MSNs (Fig. 3). H3-D2 heteromers have also been described (24); their effect on downstream signaling has not been characterized. We found no effect of RAMH treatment in vivo on MAPK or cAMP/DARPP-32 signaling in D2-expressing MSNs.
rpS6 is transiently phosphorylated at Ser 235 /Ser 236 after H3R activation in D1-MSNs (Fig. 3). Several observations suggest that this is a MAPK-dependent event. rpS6 is regulated by phosphorylation at two pairs of sites, Ser 235 /Ser 236 and Ser 240 /Ser 244 (41). Both can be phosphorylated by p70S6K via a mammalian target of rapamycin (mTOR)-dependent signaling cascade (42). MAPK signaling, on the other hand, phosphorylates Ser 235 / Ser 236 but not Ser 240 /Ser 244 via ribosomal protein S6 kinase and MSK activation (43). This parallels the pattern of phosphorylation we see here (Fig. 3). The fact that the kinetics of rpS6 phosphorylation parallel those of ERK and MSK activation in D1-MSNs with a marked but transient increase at 15 min that entirely returned to baseline by 45 min further supports the conclusion that this is a MAPK-dependent phenomenon. On the other hand, H3/D1 co-stimulation completely abolishes activation of MSK but only partially attenuates phosphorylation of rpS6 (Fig. 6, A and B). rpS6 may also be phosphorylated at Ser 235 /Ser 236 through a distinct mechanism.
H3R Regulation of Akt-GSK3␤ Signaling-Our most striking finding is the opposite regulation of Akt-GSK3␤ signaling by H3R in D1-and D2-MSNs. We observe Ser 9 phosphorylation of GSK3␤ specifically in D1-MSNs 15 min after RAMH administration (Fig. 4). This is consistent with a PI3K-dependent mechanism documented previously in culture and ex vivo (28,29). D1R activation with SKF82958 does not detectably alter GSK3␤ phosphorylation or modulate the effects of H3 activation (Fig.  6C). GSK3␤ can be phosphorylated by MAPK (44 -48), but this dissociation between MAPK regulation and GSK3␤ phosphorylation after D1/H3 co-stimulation argues against this being the primary mechanism at play here.
The effect of H3R activation in D2-MSNs is quite different. RAMH leads to dephosphorylation of GSK3␤ with a slower time course (Fig. 4). This is similar to the effect of D2R stimulation, which leads to Akt deactivation and thus to GSK3␤ dephosphorylation via a ␤-arrestin-dependent mechanism in these cells (15). Whether H3 receptors are coupled to Akt-GSK3␤ signaling by a similar molecular mechanism remains to be determined. Regardless, this dephosphorylation (and therefore presumptive activation) of GSK3␤ after H3R stimulation has not, to our knowledge, been reported previously in any cell type. Importantly, H3R activation on D2-MSNs does not recapitulate all effects of D2R stimulation; there is no detectable regulation of cAMP/DARPP-32 signaling (Fig. 1). Therefore, H3R activation leads to a unique pattern of signaling in both D1-and D2-expressing MSNs. The qualitative dissociation of molecular effects of stimulation of a single receptor indicates that H3R signaling is critically modulated by its interaction with other cellular components-either direct interactions with D1 and D2 receptors (25)(26)(27) or some other cell type-specific factor.
Phosphorylation of GSK3␤ in D1-MSNs is transient, peaking at 15 min and reverting to baseline by 45 min. In D2-MSNs, dephosphorylation of GSK3␤ develops more gradually, with only a trend apparent at 15 min but a robust effect at 45 min. Examination of GSK3␤ phosphorylation in total striatal extracts (Fig. 4B) combines these two effects into a biphasic pattern of GSK3␤ phosphorylation, with an increase at 15 min followed by a decrease at 45 min. Examination of these effects with cell type specificity, as we do here, is essential to reveal the dynamics that underlie this complex pattern.
Functional Interaction between H3 and D1 Agonists-Treatment with the H3R agonists RAMH or immepip affects neither locomotion nor stereotypy, but it eliminates the locomotor activation produced the D1 agonist SKF82958 (Fig. 5). A similar effect has been described previously in reserpinized mice (24). The mechanisms underlying this interaction, however, have remained unclear. H3R activation has no effect on intrastriatal DA (Fig. 1C), and H3-D1 co-stimulation does not produce elevated stereotypy (Fig. 5B).
A candidate molecular mechanism for this interaction is the inactivation by phosphorylation of GSK3␤ at Ser 9 that we see in D1-MSNs after RAMH treatment (Figs. 4 and 6). GSK3␤ has been shown to be necessary for the locomotor stimulatory effects of D1 agonist treatment (49). GSK3␤ inhibition by H3 agonist treatment, specifically in D1-MSNs, may produce a similar effect.
This implies that intrastriatal HA levels in vivo may regulate susceptibility to locomotor stimulation after DA agonist or psychostimulant challenge. Because striatal HA fluctuates slowly, with a circadian pattern (1,7), this would predict circadian variation in the sensitivity to D1 agonist effects. Diurnal variation in psychostimulant effects has indeed been documented (50). Of note, H3Rs exhibit a high level of basal activity, even in the absence of agonist (51). These receptors may therefore provide a source of constitutive cell type-specific GSK3␤ regulation partially independent of fluctuations in local HA concentration.
It is striking that H3R antagonists have also been reported to mitigate locomotor and other actions of psychostimulants (52)(53)(54) although not, to our knowledge, of D1-specific agonists. The ability of H3R agonist and antagonist co-administration to produce similar effects on DA agonist-induced hyperlocomotion speaks to the complexity of HA regulation of the basal ganglia circuitry and the importance of analytic approaches that isolate effects on discrete cell types.
Conclusions-The presence and importance of postsynaptic H3Rs on striatal MSNs has only been appreciated recently (22, 24 -27). The mechanisms whereby they regulate intracellular signaling and basal ganglia function remain incompletely understood. We observe complex, temporally distinct, and cell type-specific MAPK and Akt-GSK3␤ regulation in D1-and D2-MSNs. These cell type-specific effects, especially those on GSK3␤, are not apparent in analysis of whole striatum extracts; they become clear only when analysis is restricted to specific MSN subtypes. The functional importance of H3R activation is apparent in its ability to mitigate hyperlocomotion because of aberrant striatal function.
H3 receptors are increasingly studied as a pharmacological target under a range of conditions (55)(56)(57). Appreciation of their unique regulation of intracellular signaling events in MSNs will be critical to understanding their potential as a therapeutic target. The disparate regulation of GSK3␤ and rpS6 in D1-and D2-MSNs, in particular, indicates multiple points of interaction between DA and HA signaling.

Materials and Methods
Animals-Adult (2.5-to 5-month-old) male mice were used for all experiments. BAC-D2EGFP mice, which were used to analyze signaling specifically in D2-positive and D2-negative cell populations in most experiments, were originally produced by the GENSAT program at Rockefeller University and were generously provided, backcrossed extensively onto C57Bl/6, by Dr. James Surmeier (58). Double-transgenic D1-DARPP-32-FLAG/D2-DARPP-32-Myc (D1F/D2M) mice, which were used to characterize DARPP-32 signaling, have been described previously (14). All transgenic strains were backcrossed with C57BL/6J to Ն N9. Wild-type C57BL/6J mice were purchased from The Jackson Laboratory. The care and use of animals were supervised by the Yale University Institutional Animal Care and Use Committee.
Locomotor Activity and Stereotypic Behavior-Mice were placed into the behavioral room in their home cages to habituate for 20 min. Next, animals were placed individually into a clean cage with fresh bedding (18.5 inches long ϫ 14.5 inches wide ϫ 8 inches high) in a locomotor monitoring apparatus (Omnitech Electronics) and monitored for 30 min to establish a baseline. Animals were injected with saline, SKF82958, RAMH, SKF82958 ϩ RAMH, immepip, or SKF82958 ϩ immepip (8 animals/group). Locomotor activity was recorded for an additional 1 h and quantified as the number of beam breaks using Fusion software (Omnitech Electronics).
For quantification of stereotypy, mice were placed alone in a plexiglass cage to habituate for 20 min, injected i.p. with saline or drugs as above, and then videotaped individually for 1 h (11 inches long ϫ 8 inches wide ϫ 8 inches high). Various stereotypic behaviors (grooming, cage mouthing, feces mouthing, focused sniffing, digging, and forepaw flailing) were scored off-line from video by a trained observer blind to experimental conditions in four 2-min time bins spaced evenly over 1 h. The reported stereotypy score was calculated as the sum of the total time spent doing any of the measured stereotypic behaviors during these scored intervals.
Immunoprecipitation and Immunoblotting for DARPP-32-D1F/D2M mice were sacrificed by focused microwave irradiation 15 min after RAMH administration. Tissue preparation was carried out as described previously (14). Striata were dissected, sonicated in 500 l of homogenization buffer, and centrifuged for 20 min at 13,000 rpm. An aliquot of total striatal homogenate was separated from each sample and set aside (the "total" sample). The remaining homogenate was added to 50 l of anti-FLAG-conjugated agarose beads (Sigma, EZView red) and rotated overnight at 4°C. FLAG beads were washed twice and eluted in 2ϫ sample buffer. ␤-Mercaptoethanol was added to the flow-through from each sample, which was then transferred to a 50-l slurry of anti-Myc-conjugated Sepharose beads (Cell Signaling Technology) and rotated overnight at 4°C. Myc beads were washed three times and eluted in 3ϫ sample buffer with ␤-mercaptoethanol. Flowthrough was collected, and all samples were frozen at Ϫ80°C until processing. Samples from total, FLAG, and Myc extracts were electrophoresed through 4 -20% Tris/glycine midi gels (Life Technologies) and electroblotted overnight onto PVDF membranes. Membranes were blocked for 1 h with 5% nonfat dry milk, incubated overnight with Thr(P) 34 DARPP-32 Ab (1:1000, Greengard laboratory, Ab cc500), and then washed in the same buffer. HRPconjugated anti-rabbit secondary (1:2000, Pierce) was added, and incubation was continued for 1 h at room temperature. Blots were developed with Pico ECL solution (Pierce) and imaged using the Bio-Rad Chemi-doc XRSϩ imaging system. Blots were immediately stripped for 3 h in stripping buffer (25 mM glycine, 2% SDS (pH 2)) at 50°C. Blocker was added for 1 h, followed by total DARPP-32 Ab (1:2000, Greengard laboratory, mAb 6a) overnight at 4°C. Membranes were incubated with HRP-conjugated antimouse secondary Ab (1:2000, Pierce) for 1 h at room temperature and developed with Pico ECL solution using the Bio-Rad Imager. Thr 75 blots were processed according to the same protocol using primary Thr(P) 75 DARPP-32 Ab (1:1000, Ab cc911) and secondary HRP-conjugated anti-rabbit (1:1000, Pierce).
Immunoblot Analysis-All bands were quantified and normalized to corresponding total respective non-phospho-protein per sample. Normalized values were adjusted to the average of normalized control values on each blot. Values were excluded as outliers when they varied by more or less than 2 standard deviations from the overall mean for the experiment.
Confocal Imaging and Cell Counting-BAC-D2EGFP mice were deeply anesthetized with ketamine (100 kg/kg)/xylazine (10 mg/kg) after 15 or 45 min of RAMH or saline administration and then transcardially perfused with 4% paraformaldehyde supplemented with 0.1 mM NaF. Brains were fixed overnight in paraformaldehyde 4% ϩ NaF and then stored at 4°in 30% sucrose. Brains were cut at 20 m on a cryostat (Leica). Slices were stored in a solution containing 30% glycerin, 30% ethylene glycol, and 1ϫ TBS supplemented with 0.1 mM NaF.
For staining, slices were washed three times for 10 min in TBS (pH 7.4), incubated for 1 h at room temperature in TBS with 0.2% Triton X-100 and 5% donkey serum, and then incubated overnight at room temperature in the same solution with one of the following antibodies: rabbit anti-phospho-MSK1-Thr 581 (1:500, Cell Signaling Technology, 9595P), rabbit anti-Ser(P) 235 /Ser(P) 236 -rpS6 (1:400, Cell Signaling Technology, 4858S), and rabbit anti-Ser(P) 240 /Ser(P) 244 -rpS6 (1:400, Cell Signaling Technology, 5364P). Detection was performed by incubating for 1 h at room temperature with an Alexa Fluor 555-labeled donkey anti-rabbit secondary antibody (1:400, Life Technologies) in blocking solution. For detection of Ser(P) 9 GSK3␤, before blocking and incubation with the primary antibody, slices were incubated with Bloxall at room temperature for 20 min (Vector Laboratories). Then secondary detection was performed with a tyramide signal amplification-Cy3 kit (PerkinElmer Life Sciences) according to the instructions of the supplier. Sections were washed three times with PBS and then mounted in Vectashield (Vector Laboratories).
Confocal imaging was performed on an Olympus Fluoview FV-1000 confocal microscope. Z stacks (15 m) were collected from the dorsal striatum from five sections from each mouse. Images were taken from the central dorsal striatum (see Fig. 2C for placement of a typical confocal image within the dorsal striatum).
For each of the phosphorylated proteins (P-MSK, P-rpS6, and P-GSK3␤), we counted cells throughout the Z stack that showed clear immunostaining above background in the cell body. Each cell was categorized as either co-expressing EGFP (thus a presumptive D2-MSN) or not co-expressing EGFP (thus a presumptive D1-MSN). All clearly stained cells in each Z stack were categorized and quantified. Immunopositive cells that could not be unambiguously categorized as D1-or D2-MSNs were excluded. All counting was done blind to pharmacological treatment and time point. The total number of cells was averaged for each mouse for analysis. n for each experiment thus reflects the number of mice, not slices. Tissue at the 15-and 45-min time points was collected in separate cohorts of mice, each with a matched saline control group. Counts of GFP-positive and GFP-negative cells were normalized to the corresponding saline group for analysis.
Microdialysis-DA microdialysis was performed as described previously (1). Mice were surgically implanted unilaterally with guide cannulae targeted to the dorsal striatum (anterior-posterior, ϩ0.5 mm; medial-lateral, 2.0 mm; dorsalventral, Ϫ2.2 mm) (59) under ketamine/xylazine anesthesia using standard stereotaxic technique. Guide cannulae were affixed to the skull using bilateral skull screws and Cerebond skull fixture adhesive (Plastics One, Roanoke, VA). Dummy cannulae were inserted into the guide cannulae during recovery to ensure patency. C57BL/6J mice recovered from surgery for 3-5 days before continuing with microdialysis. Following the recovery period, a microdialysis probe (2 mm, CMA-7, 6-kDa cutoff; CMA Microdialysis, Stockholm, Sweden) was inserted through the guide cannula to a depth of Ϫ4 mm relative to the bregma (i.e. 2 mm below the tip of the guide cannula), and mice were left in the home cage for 20 -24 h. The next day, the probe was connected to a 2.5-ml Hamilton syringe and continuously perfused with artificial cerebrospinal fluid (Harvard Apparatus, Holliston, MA) at a rate of 2 l/min using a programmable infusion pump (CMA Microdialysis). Dialysate was collected in 20 l/10 min fractions on ice and stored at Ϫ80°C for later analysis. Microdialysis was performed in the home cage. DA was measured using HPLC with electrochemical detection as described previously (1). After microdialysis, a small amount of toluidine blue was injected through the cannula. Mice were euthanized, and their brains were removed, sliced, and examined to confirm cannula placement. All cannulae were successfully targeted to the dorsal striatum as intended (n ϭ 8).
Data Analysis-Statistical analysis was performed using Graph Pad 6.0, except for three-way RM-ANOVA of locomotor activity (Fig. 5), which was performed using SPSS 21 (IBM). Values are expressed as mean Ϯ S.E. and compared using Student's t test, one-way ANOVA, two-way or repeated measures two-way ANOVA, followed by Sidak's post-hoc test, as indicated in each case under "Results" and in the figure legends. Differences among experimental conditions were considered statistically significant when p Ͻ 0.05.
Author Contributions-M. R. designed and performed experiments, analyzed data, and wrote and edited the manuscript. L. R. F., K. D. H., R. S., and V. P. performed experiments and analyzed data. A. C. N. contributed unique reagents and edited the manuscript. C. P. designed and supervised experiments, analyzed data, and wrote and edited the manuscript.