Lipopolysaccharide (LPS)-stimulated iNOS Induction Is Increased by Glucosamine under Normal Glucose Conditions but Is Inhibited by Glucosamine under High Glucose Conditions in Macrophage Cells*

We investigated the regulatory effect of glucosamine (GlcN) for the production of nitric oxide (NO) and expression of inducible NO synthase (iNOS) under various glucose conditions in macrophage cells. At normal glucose concentrations, GlcN dose dependently increased LPS-stimulated production of NO/iNOS. However, GlcN suppressed NO/iNOS production under high glucose culture conditions. Moreover, GlcN suppressed LPS-induced up-regulation of COX-2, IL-6, and TNF-α mRNAs under 25 mm glucose conditions yet did not inhibit up-regulation under 5 mm glucose conditions. Glucose itself dose dependently increased LPS-induced iNOS expression. LPS-induced MAPK and IκB-α phosphorylation did not significantly differ at normal and high glucose conditions. The activity of LPS-induced nuclear factor-κB (NF-κB) and DNA binding of c-Rel to the iNOS promoter were inhibited under high glucose conditions in comparison with no significant changes under normal glucose conditions. In addition, we found that the LPS-induced increase in O-GlcNAcylation as well as DNA binding of c-Rel to the iNOS promoter were further increased by GlcN under normal glucose conditions. However, both O-GlcNAcylation and DNA binding of c-Rel decreased under high glucose conditions. The NF-κB inhibitor, pyrrolidine dithiocarbamate, inhibited LPS-induced iNOS expression under high glucose conditions but it did not influence iNOS induction under normal glucose conditions. In addition, pyrrolidine dithiocarbamate inhibited NF-κB DNA binding and c-Rel O-GlcNAcylation only under high glucose conditions. By blocking transcription with actinomycin D, we found that stability of LPS-induced iNOS mRNA was increased by GlcN under normal glucose conditions. These results suggest that GlcN regulates inflammation by sensing energy states of normal and fuel excess.

Nitric oxide (NO) has been implicated in inflammatory mediator and inducible NO synthase (iNOS) is expressed predominantly by microglia and macrophages by sensing danger or foreign signals. NO leads macrophages with cytostatic or cytotoxic activity against viruses, bacteria, fungi, and protozoa (1). However, the high levels of NO produced by activated macrophages may also harm healthy cells.
Glucosamine (2-amino-2-deoxy-D-glucose; GlcN) 3 is a natural sugar that is synthesized from glucose through the hexosamine biosynthetic pathway (HBP) by virtually all cells. Entry into the HBP is catalyzed by the first and rate-limiting enzyme glutamine: fructose-6-phosphate amidotransferase (2). Exogenous GlcN, which enters the HBP bypassing fructose-6phosphate amidotransferase is taken into cells by glucose transporters (GLUT-1, GLUT-2, and GLUT-4) after which it is phosphorylated and further metabolized (3). Increased GlcN elevates UDP-N-acetyl-GlcN (UDP-GlcNAc), a direct substrate for the transfer of single O-GlcNAc to nuclear and cytosolic proteins by O-GlcNAc transferase (4 -6). GlcN or HBP has been implicated in insulin signaling through this O-GlcNAcylation mechanism (7,8).
The immune regulatory functions of GlcN have been demonstrated: GlcN may exhibit a therapeutic effect in experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis (9). At the cellular level, GlcN inhibits interleukin-1␤ (IL-1␤)-induced nuclear factor-B (NF-B) activation in chondrocytes (10,11). As well, GlcN inhibits lipopolysaccharide (LPS)-induced NO production in RAW 264.7 macrophages and microglia (10 -14). Moreover, GlcN exhibits neuroprotective and anti-inflammatory effects in a model of middle cerebral artery occlusion via suppression of NF-B activity (15). More recently, we demonstrated that GlcN by regulating c-Rel O-GlcNAcylation and subsequent NF-B activity in both microglia and macrophage cells (16). Activation of macrophages or microglia by LPS stimulates mitogen-activated protein (MAP) kinases and NF-B. MAP kinases and NF-B are both strongly implicated in the regulation of iNOS expression by LPS (17)(18)(19)(20). NF-B is normally sequestered in the cytoplasm by inhibitors of NF-B, inhibitor of B (IB) proteins including IB␣ and IB␤. In response to stimulation, IB becomes phosphorylated and is subjected to degradation after ubiquitination. This accompanies NF-B translocation to the nucleus where the protein activates transcription (21). Vertebrate NF-B transcription complexes can be any of a variety of homo-and heterodimers formed by the subunits RelA (p65), RelB, c-Rel, p50 (a product of p105), and p52 (a product of p100) (22,23). These complexes bind to DNA regulatory sites and usually activate specific target gene expression. The target gene specificity of NF-B is thought to arise from a number of factors. Some factors include the specific NF-B complexes that are in different cell types, the distinct B target site binding specificities of different NF-B complexes, and the different protein-protein interactions and posttranslational modifications that NF-B complexes (24,25).
It is well established that most metabolic diseases are directly or indirectly associated with a low-grade chronic inflammation that causes complications such as nephropathy, neuropathy, retinopathy, and atherosclerosis (26). Therefore, the excessive caloric intake might be associated with the pathogenesis of both obesity-related complications and chronic inflammation. Indeed, free fatty acids have been shown to activate the LPS receptor, Toll-like receptor (TLR) 4 (27). Free fatty acid may also induce the production of proinflammatory cytokines by macrophages (27). High-glucose concentrations are also involved in inflammatory processes (28,29). LPS modulates the generation of an inflammatory mediator in macrophages in hyperglycemic models (30). Therefore, inflammatory responses are obviously associated with glucotoxicity in diabetic conditions. Hyperglycemia-associated metabolic syndrome promotes abnormally elevated protein O-GlcNAcylation, which participates in the glucotoxicity (31)(32)(33). The association between hyperglycemia and metabolic syndrome-associated inflammation is well established yet the involvement of O-GlcNAcylation in those events is obscure. Therefore, exploring the regulation of O-GlcNAcylation and its functional implications in inflammatory regulation under different glucose settings is important. In this study, we investigated the potential of both pro-and anti-inflammatory effects of GlcN to regulate NO/iNOS induction as well as O-GlcNAcylation in macrophage cells under normal and high glucose culture conditions. GlcN demonstrated an inverse effect on LPS-mediated iNOS induction and O-GlcNAcylation and DNA binding of c-Rel between normal and high glucose conditions. Therefore, GlcN or the HBP metabolic pathway likely regulates inflammation and the related signaling pathway depends on nutrient availability. Increased by GlcN under  Normal Glucose Culture Conditions but Inhibited by GlcN  under High Glucose Culture Conditions in RAW264.7 Macrophage Cells-Previous reports by our group showed that GlcN inhibits LPS-induced NO production (13,15). In this report, we investigated the dose-dependent effect of GlcN on LPS-caused NO synthesis under increasing glucose concentrations. RAW264.7 cells were incubated with culture medium containing zero, normal (5 mM), or high glucose (25, 50, or 100 mM) in the presence or absence of LPS (100 ng/ml) and/or GlcN (5 mM). Concentrations higher than 20 mM were considered as analogous to a diabetic condition within the cell culture system. The nitrite levels (an index of NO production) in culture medium were compared after 24 h. LPS produced detectable NO generation at concentrations higher than 5 mM glucose conditions. Furthermore, LPS-induced NO generation was dose dependently increased at various glucose concentrations (Fig. 1A). The addition of GlcN 2 h before LPS administration resulted in a complicated effect on NO generation as per glucose concentration. GlcN increased LPS-mediated NO generation at normal glucose conditions but GlcN strongly inhibited LPS-mediated NO generation at concentrations higher than 25 mM glucose (Fig. 1A).

LPS-mediated NO Production Was
Next, we examined dose effects of GlcN on NO production and cell viability in RAW264.7 cells in response to LPS. The level of nitrite in culture medium at 24 h of LPS stimulation was increased by GlcN in a dose-dependent manner at normal glucose culture conditions. However, LPS-induced NO production was dose dependently inhibited by GlcN at 25 mM glucose conditions (Fig. 1B, upper panels) as previously demonstrated (13,15). GlcN inhibited cell death that was induced by LPS under 25 mM glucose conditions. Conversely, GlcN yielded a minimal effect on cell death under 5 mM glucose conditions (Fig. 1B, lower panels). Morphological examinations indicated that GlcN protected cells against LPS-mediated cell death at 48 h under both 5 and 25 mM glucose conditions (Fig. 1C).

Influences of GlcN on LPS-mediated Production of iNOS and Other Inflammatory Genes under Normal and High Glucose
Culture Conditions Were Examined-The effect of GlcN on LPS-mediated induction of proinflammatory genes was examined under normal or high glucose conditions. Raw264.7 cells were incubated with culture medium containing normal glucose (5 mM) or high glucose (25 mM) in the presence or absence of LPS (100 ng/ml) and/or GlcN (5 mM). LPS-initiated iNOS expression at 24 h was increased by GlcN under 5 mM glucose culture conditions. Conversely, GlcN inhibited iNOS and COX-2 protein expression in cells under 25 mM glucose culture conditions ( Fig. 2A). Next, we examined the effect of GlcN on the activity of the iNOS promoter under ambient glucose conditions. GlcN suppressed the activity of the iNOS promoter at 25 mM glucose conditions, as previously described (15). In contrast, GlcN stimulated reporter activity of the iNOS promoter under 5 mM conditions (Fig. 2B).
Levels of mRNA encoding iNOS, COX-2, IL-6, TNF-␣, and IL-1␤ were increased by LPS and their expressions were strongly inhibited by GlcN under 25 mM conditions. At 5 mM culture conditions, GlcN stimulated LPS-induced iNOS mRNA. However, LPS-stimulated mRNA expression of COX-2, IL-6, and TNF-␣ were all not significantly influenced by GlcN at 5 mM glucose conditions. LPS-mediated expression of IL-1␤ was inhibited by GlcN at 5 mM conditions (Fig. 2C). We verified the GlcN effect on LPS-induced iNOS mRNA expression using primary cultured mouse peritoneal macrophages; GlcN was inhibited at 25 mM conditions but stimulated at 5 mM conditions (Fig. 2D). We have verified that GAPDH expression was not changed by glucose and/or GlcN (data not shown).

Time Course iNOS Induction in Response to LPS and/or GlcN under Normal or High Glucose Culture Conditions Was
Examined-We examined the time course of expression of mRNA encoding iNOS, and that of protein expression per se, in RAW264.7 cells exposed to LPS (100 ng/ml) under 5 or 25 mM glucose culture conditions. Furthermore, we determined how GlcN affected induction of such expression. iNOS protein was detected as early as 6 h LPS stimulation at both 5 and 25 mM glucose. GlcN stimulated iNOS expression at 5 mM glucose but was relatively ineffective at 25 mM glucose conditions (Fig. 3A). LPS-induced expression of iNOS was more distinct at 12 h. GlcN was further stimulated at concentrations of 5 mM glucose but slightly inhibited under 25 mM glucose. Likewise, the increase in iNOS mRNA by LPS was inhibited by GlcN at 12 and 24 h of LPS stimulation under 25 mM glucose conditions. Contrastingly, LPS-induced iNOS mRNA was stimulated by GlcN at all time points examined at 5 mM glucose concentration (Fig. 3B).
Glucose and/or GlcN-regulated iNOS, C/EBP-␦, C/EBP-␤, and GRP78 Protein Expression in an Independent Manner-To explore any dependence of possible differential regulation of . Cells were cultured with DMEM containing 5 or 25 mM glucose for 4 h. Cells were then treated at the indicated concentrations of GlcN for 2 h followed by stimulation with 0.1 g/ml of LPS (B and C). NO production at 24 h was measured by measuring nitrite levels in the culture medium (B, upper panels) and cell viability at 72 h was determined by an MTT assay and expressed as a percentile compared with untreated control (B, lower panels). Cell phenotype at 48 h was observed under a phase-contrast microscope (C). Shown are representatives of three independent experiments. All values are mean Ϯ S.E. *, denotes significantly increased from the untreated control; **, indicates significantly increased from LPS-stimulated conditions; #, indicates significantly decreased from LPS-stimulated conditions; †, indicates decreased from untreated control.
GlcN for iNOS induction on glucose-regulated transcription factors or ER stress responses, we examined iNOS, C/EBP-␦, C/EBP-␤, and GRP78 protein induction levels in RAW264.7 cells. Cells cultured in increasing concentrations of glucose (0, 5, 25, 50, and 100 mM) displayed increased iNOS expression in response to LPS in a dose-dependent manner. Under the same conditions, the expression of C/EBP-␦, C/EBP-␤, and GRP78 were all reduced by glucose in a dose-dependent manner both in the absence and presence of LPS (Fig. 4A). C/EBP-␤ expression was less distinctly reduced by glucose in the presence of LPS.
Next, we determined the effect of GlcN on the expression of C/EBP-␦, C/EBP-␤, and GRP78 under 5 or 25 mM glucose conditions. C/EBP-␤ was not significantly influenced by LPS and/or GlcN at 5 or 25 mM glucose. At 5 mM glucose, the expression of C/EBP-␦ was stimulated by LPS but inhibited by GlcN. However, at 25 mM glucose, GlcN further increased LPSincreased C/EBP-␦ protein expression (Fig. 4B). GRP78 protein was expressed in a lower level at 25 mM glucose than at 5 mM glucose conditions. However, GRP78 expression was significantly increased by 5 mM GlcN in 25 mM glucose with or without LPS. In addition, GRP78 was slightly increased by GlcN in the presence of LPS in 5 mM glucose condition.
The Activation of MAPKs and IB␣ in Response to LPS and/or GlcN under 5 or 25 mM Glucose Conditions Was Investigated-Activation of mitogen-activated protein kinase (MAPK) signaling by LPS, and the effect of GlcN on such sig-naling, was examined 30 min, 2 h, and 12 h after LPS (100 ng/ml) addition under 5 or 25 mM glucose culture conditions. LPS increased phosphorylation of ERK1/2, p38, and JNK in RAW264.7 cells at both 5 and 25 mM glucose conditions. GlcN did not significantly affect the extent of any LPS-induced phosphorylation of ERK1/2, p38, and JNK 30 min after LPS addition under 5 or 25 mM glucose. A reduction in ERK1/2 phosphorylation by GlcN was evident 12 h after LPS addition under both 5 and 25 mM glucose conditions. LPS-induced phosphorylation of p38 and JNK was reduced in the presence of GlcN at 12 h after LPS addition at 25 mM glucose but not at 5 mM glucose (Fig. 5, A and B). IB␣ was both phosphorylated and degraded by LPS at 30 min. GlcN did not influence degradation nor phosphorylation of IB␣ at either 5 or 25 mM glucose conditions.
GlcN Suppressed NF-B Activation in Response to LPS under 25 mM Glucose, but Not under 5 mM Glucose Environment-We compared the effect of GlcN on LPS-induced NF-B activation between normal and high glucose conditions. LPS stimulated nuclear translocation of c-Rel and p50 in RAW264.7 cells maintained at both 5 and 25 mM glucose culture conditions. Conversely, p65 nuclear translocation was increased at only 25 mM glucose conditions (Fig. 6A). Nuclear translocations of p65, c-Rel, and p50 in response to LPS were inhibited by GlcN at 25 mM glucose, but were not significantly influenced under 5 mM glucose (Fig. 6A). Next, we examined the effects of LPS and/or GlcN on NF-B activation using the NF-B-reporter construct. LPS activated the NF-B reporter at 25 mM glucose but less strongly at 5 mM glucose. GlcN inhibited NF-B activity under 25 mM glucose conditions but did not significantly exhibit any influence at 5 mM glucose conditions (Fig. 6B).
To determine whether LPS and/or GlcN directly regulated the binding of Rel family proteins to promoter DNA, we assayed the ability of Rel proteins to bind to a biotinylated oligonucleotide corresponding to the distal NF-B site. This site (Ϫ957 to Ϫ977 in the mouse iNOS promoter) is important regarding gene induction by LPS (34). Stimulation with LPS for 24 h induced increases in the binding levels of both c-Rel and p50 at both 5 and 25 mM glucose concentrations. Furthermore, p65 binding also increased at 25 mM glucose. LPS-stimulated binding of p65, c-Rel, and p50 were all suppressed by GlcN at 25 mM glucose yet these bindings were all not affected by GlcN at 5 mM glucose (Fig. 6C). To examine the physiological relevance of such DNA binding, we performed chromatin immunoprecipitation (ChIP) assays. LPS induced an increase in the binding of c-Rel to the endogenous iNOS promoter both in 5 and 25 mM glucose conditions. In contrast, binding was suppressed by GlcN at 25 mM glucose but not influenced under 5 mM glucose conditions (Fig. 6D). This suggests a regulatory role for GlcN in LPS-mediated iNOS induction that may be controlled by regulating the chromatin mobilization of c-Rel proteins.

LPS-dependent Increase in c-Rel O-GlcNAcylation Is Inhibited by GlcN at 25 mM Glucose but Increased by GlcN at 5 mM
Glucose Conditions-We have previously reported that LPS and/or GlcN regulate iNOS induction via post-translational O-GlcNAcylation of c-Rel (16). We performed wheat germ agglutinin (WGA) pulldown assays using WGA-conjugated agarose beads that specifically precipitate proteins containing N-acetylglucosamine or N-acetylneuramic acid. RAW264.7 cells were treated with LPS (100 ng/ml) and/or GlcN (5 mM) for 24 h under 5 or 25 mM glucose conditions. O-GlcNAcylation of total cell lysates was stimulated by GlcN at 5 mM glucose compared with no significant changes by GlcN at 25 mM glucose (Fig. 7A, upper panels). The amount of c-Rel protein in the WGA precipitate was increased by LPS and this increase was inhibited by GlcN at the 25 mM glucose conditions, as previously described (16). However, WGA binding of c-Rel in response to LPS was dramatically increased by GlcN at the 5 mM glucose conditions (Fig. 7A, lower panels). These results suggest that O-GlcNAcylation of c-Rel or a protein that interacts with c-Rel is differentially regulated by GlcN depending on glucose concentrations. We further confirmed O-GlcNAcylation changes of total and c-Rel proteins in response to LPS and/or GlcN under 5 mM glucose conditions by labeling O-GlcNAc with galactosyltransferase using [ 3 H]galactose as a substrate. O-GlcNAcylation of total nucleocytoplasmic proteins was increased by GlcN regard- less of LPS presence (Fig. 7B, upper panel). O-GlcNAcylation of c-Rel determined by [ 3 H]galactose labeling demonstrated that c-Rel O-GlcNAcylation at 5 mM glucose was slightly increased by GlcN and further increased by LPS plus GlcN (Fig.  7B, lower panels).
Next, we compared O-GlcNAcylation of nuclear and cytosolic c-Rel and p65 in response to LPS and/or GlcN under 5 or 25 mM glucose. Both nuclear and cytosolic c-Rel responded similarly to LPS and/or GlcN at two different glucose conditions for O-GlcNAcylation. c-Rel O-GlcNAcylation was increased by LPS but this increase was inhibited by GlcN at 25 mM glucose conditions. However, GlcN further increased the O-GlcNAcylation of c-Rel in response to LPS at 5 mM glucose conditions (Fig. 7C). O-GlcNAcylation of p65 was increased by GlcN both in the nucleus and cytosol at 5 mM glucose, but it was not significantly influenced by LPS and/ or GlcN at 25 mM glucose. These results suggest that O-GlcNAcylation is not likely associated with nuclear translocations of c-Rel and p65.
O-GlcNAcylation of c-Rel may regulate the association between other members of Rel proteins. An antibody against c-Rel co-immunoprecipitated p65 and p50 in both nuclear and cytosolic fractions. The association between c-Rel and p50 in the nucleus increased in response to LPS; GlcN inhibited this association in 25 mM glucose but GlcN did not effect this association in 5 mM glucose (Fig. 7D).   . Effect of GlcN on LPS-induced signaling pathway under 5 or 25 mM glucose conditions. RAW264.7 cells were incubated with DMEM containing glucose at 5 or 25 mM for 4 h, treated with 5 mM GlcN for 2 h, and stimulated with 0.1 g/ml of LPS for 0.5 or 12 h. A, Western blots were performed using p-ERK1/2, ERK1/2, p-JNK1/2, JNK1, p-p38, p38, IB-␣, and p-IB-␣-specific antibodies. B, relative densitometric intensity for p-ERK1/2, p-JNK1/2, or p-p38 was quantitatively measured by normalizing to ERK1/2, JNK1, or p38 levels, respectively. Blots are representatives of three independent experiments. All values are mean Ϯ S.E. *, denotes significantly increased from the untreated control; #, indicates significantly decreased from LPS-stimulated conditions. conditions. Conversely, PDTC exhibited no, or a minimal effect, on LPS-or LPS plus GlcN-mediated NO/iNOS induction under 5 mM glucose conditions (Fig. 8, B and C). LPS plus GlcN-induced expression of COX-2 proteins was inhibited by 20 M PDTC under 5 mM glucose.
Next, we examined O-GlcNAcylation changes of c-Rel and p65 by PDTC in response to LPS and/or GlcN under ambient glucose culture conditions. The O-GlcNAcylation of c-Rel in response to LPS plus GlcN was slightly (less than 2-fold) inhibited by PDTC at 5 mM glucose conditions. However, the O-GlcNAcylation of c-Rel by LPS was dramatically (more than 15-fold) decreased by PDTC at 25 mM glucose conditions (Fig.  8E). O-GlcNAcylation of p65 was not significantly influenced by PDTC under both 5 and 25 mM glucose conditions. GlcN Increased the Stability of iNOS mRNA under 5 mM Glucose Culture Conditions-Because the expression of iNOS is regulated post-transcriptionally as well as transcriptionally, we examined the iNOS mRNA degradation. As an inhibitor of transcription, actinomycin D (ActD) was added to RAW264.7 cells to inhibit new synthesis of iNOS at 12 h of LPS or LPS plus GlcN stimulation. Cells were harvested at 2, 4, 6, and 8 h after the addition of ActD. At 5 mM glucose, iNOS mRNA was reduced in LPS-stimulated conditions in a time-dependent manner by an addition of ActD, whereas the iNOS mRNA level was not significantly changed by ActD in LPS plus GlcN-stimulated conditions (Fig. 9A, left graph). Contrarily, LPS-induced iNOS mRNA was not significantly changed by ActD, whereas iNOS mRNA in response to LPS plus GlcN displayed a time course decrease at 25 mM glucose conditions (Fig. 9A, right  graph).
Tristetraprolin (TTP) is involved in mRNA degradation of iNOS (35). TPP mRNA was increased 6 h after LPS stimulation at 5 mM glucose conditions. GlcN significantly reduced the LPS-mediated increase of TTP (Fig. 9B). A, nuclear protein extracts were prepared and p65, c-Rel, or p50 expressions were measured by Western blotting and relative densitometric intensity was quantitatively measured by normalizing to HDAC1 protein. B, RAW264.7 cells were transfected with NF-B-reporter constructs and preincubated with 5 or 25 mM glucose containing medium for 4 h and pretreated with 5 mM GlcN for 2 h. Cells were then stimulated with 0.1 g/ml of LPS for 24 h. Luciferase activities were measured from cell lysates and relative values were demonstrated. C, cells were incubated with 5 or 25 mM glucose containing media for 4 h with or without GlcN for 2 h followed by stimulation with LPS for 24 h. Total cell lysates were prepared and binding of Rel proteins to the biotinylated NF-B probe was examined by pulling down using streptavidin-agarose beads. Bound proteins were determined by Western blotting using specific antibodies for c-Rel, p65, and p50. D, formaldehyde crosslinked chromatin DNA was immunoprecipitated with anti-c-Rel antibody and eluted DNA was quantified by real-time PCR or standard reverse transcriptase PCR (RT-PCR) for the iNOS promoter. Input represents each PCR product obtained from 2% of the preimmunoprecipitated DNA. Blots are representatives of three independent experiments. All values are mean Ϯ S.E. *, denotes significantly increased from the untreated control; **, indicates significantly increased from LPS-stimulated conditions; #, indicates significantly decreased from LPS-stimulated conditions.

Discussion
Among the most critical factors of survival are the ability to withstand starvation and an effective immune response to pathogens. Inflammation is basically triggered by nutrient surpluses, and inflammatory responses and metabolic disorders share common signaling molecules and pathways. A large body of emerging evidence suggests that nutrient signaling through the HBP serves an important role in cellular adaptation to fluctuations in nutrient availability even at physiological glucose concentrations (36). Our previous study (37) discussed the distinct role of the GlcN, in which metabolic processes are allowed to adapt to nutritional states of normal and fuel excess: GlcN impaired the insulin response when fed to normal diet-fed mice yet it alleviated insulin resistance in high fat diet-fed mice. The results in this report indicate that increased GlcN addition served an inverse role in regulating LPS-mediated inflammation in macrophage depending on glucose availability. GlcN increased LPS-mediated iNOS induction under normal (5 mM) glucose culture conditions, whereas it strongly inhibited LPSmediated iNOS induction under high (25 mM) glucose condi-tions. The direct effect of glucose availability on inflammatory response at the cellular level has been described. LPS-induced NO/iNOS induction was increased at high glucose-cultured cells than normal glucose-cultured cells via enhancing activation of NF-B (30,38,39). We newly propose in this report that glucose availability sensed by HBP flux influences the regulation of inflammation.
We postulate that the endoplasmic reticulum (ER) might be a point for the sensing of metabolic stress and the translation of that stress into inflammatory signaling and consequent responses. Indeed, the ER could be considered an essential point of integration between nutrient and pathogen responses as ER is very sensitive to glucose and energy availability, lipids, pathogens, and pathogen-associated components. It has been demonstrated that elevated concentrations of glucose induce ER stress by increasing HBP flux in skeletal muscle and aortic endothelial cells (40). According to our results, whereas GlcN increased GRP78 expression, exposure to high glucose reduced the expression of GlcN-induced GRP78 in macrophage cells. Therefore, metabolic stress and HBP may impose a compli- Total cell lysates or c-Rel immunoprecipitates were subjected to galactosyltransferase labeling using [ 3 H]UDP-galactose as a substrate and exposed to X-ray film for autoradiography. C and D, cells cultured at 5 or 25 mM glucose were treated with GlcN followed by stimulation with LPS for 24 h. Nuclear and cytosolic fractions were prepared and subjected to precipitation (IP) with WGA-conjugated agarose beads (C) or anti-c-Rel antibody (D). Total or precipitated proteins were immunoblotted by c-Rel, p65, p50, and O-GlcNAc (CTD110.6) antibodies. Blots are representatives of three independent experiments. cated effect on ER stress in macrophage cells. Currently, any consequent link between ER stress and inflammatory regulation under normal or high glucose conditions is elusive.
Several transcriptional factors, such as NF-B, Fos/Jun, CREB, C/EBP, GAS, and IRF, have been described to be involved in the regulation of iNOS gene expression (41)(42)(43)(44). Among these transcriptional factors, C/EBP-␦ may be a critical mediator in glucose-mediated regulation of iNOS gene expression in response to LPS (39). We found that C/EBP-␦ protein expression was reduced as the glucose concentration increased with or without LPS. This demonstrated an inverse correlation with an LPS-induced increase of iNOS expression. In addition, the increased expression of C/EBP-␦ in response to LPS is reduced by GlcN at 5 mM conditions but increased by GlcN under 25 mM conditions. This suggests that C/EBP-␦ imparts a negative role in regulating inflammation in response to LPS and/or GlcN. Anti-inflammatory function of C/EBP-␦ has been studied: a C/EBP-␦ deficiency increases up-regulation of inflammatory genes in pancreatic beta cells (45). However, the role of C/EBP-␦ in glucose-and/or GlcN-mediated inflammatory regulation may depend on type of cell or type and duration of the insult and has to be clarified in further research.
In our previous report (13,16), GlcN inhibited LPS-induced NF-B and MAPK activation in response to LPS under high glucose conditions. In this study, we found that LPS-induced phosphorylation of MAPKs and IB␣ were not influenced by GlcN under 5 mM glucose conditions. Instead, GlcN differentially regulated the LPS-induced NF-B activation between 5 and 25 mM glucose conditions. GlcN suppressed the LPS-induced NF-B activation under 25 mM glucose conditions, whereas GlcN did not influence the activity of NF-B caused by LPS under 5 mM glucose culture conditions. These results A, nitrite levels were measured. B and C, whole cell lysates were prepared and analyzed by Western blotting using iNOS and COX-2 specific antibodies (B). The relative densitometric intensity was quantitatively measured by normalizing to GAPDH protein (C). D, nuclear extract was prepared and DNA binding to a 32 P-labeled NF-B probe was measured by EMSA (upper panels). The relative densitometric intensity was quantitatively measured and demonstrated (lower graphs). E, total cell lysates were prepared and subjected to precipitation with WGA-conjugated agarose beads. Precipitated proteins were immunoblotted by c-Rel, p65, and GAPDH antibodies (upper blots). Relative densitometric intensity of O-GlcNAcylation of c-Rel and p65 subunits were quantitatively measured and normalized to GAPDH levels (lower graphs). Data are representatives of three independent experiments. All values are mean Ϯ S.E. #, indicates significantly decreased from LPS-stimulated conditions; *, denotes significantly increased from the untreated control; **, indicates significantly increased from LPSstimulated conditions. imply a distinct role of GlcN in regulating NF-B activity according to glucose availability.
Increased GlcN results in O-GlcNAcylation, which mediates important features of transcriptional regulation. Previously, we have shown that GlcN inhibits LPS-induced NF-B activation by regulating O-GlcNAcylation of p65 or c-Rel in response to LPS (15,16). O-GlcNAcylation of p65 or c-Rel and their subsequent transcriptional activities may be differentially regulated by GlcN under ambient glucose conditions. O-GlcNAcylation and DNA binding of c-Rel in response to LPS was dramatically increased by GlcN under 5 mM glucose conditions. However, increased O-GlcNAcylation did not impose any increased association with other Rel proteins. A major question that arises then is whether the increased c-Rel binding to an iNOS promoter is actually sufficient to induce increased iNOS expression. The O-GlcNAcylation changes or recruitment of other transcription factors to the iNOS promoter might also be required for increased iNOS induction. Although this remains a possibility, our attempts to obtain evidence for changed recruitment or O-GlcNAcylation of RNAPII, CBP, p300, or mSin3A (in response to LPS plus GlcN under normal glucose conditions) proved unsuccessful (data not shown).
PDTC, an antioxidant and NF-B inhibitor, strongly inhibited LPS-mediated NO/iNOS induction and NF-B activation when cells were cultured under 25 mM glucose conditions, as we and others previously reported (13,46). However, LPS-mediated NO/iNOS induction was not significantly altered by PDTC under 5 mM glucose conditions. Furthermore, PDTC did not inhibit the DNA-binding activity of NF-B to the binding site of the iNOS promoter at 5 mM glucose conditions. This may be attributable to the differential regulation Although an increased responsiveness of LPS-induced NF-B activity by GlcN under 5 mM glucose conditions seems to contribute to the increased iNOS induction, other additional regulatory mechanisms may exist as well. We proposed the changes of iNOS mRNA stability as an additional scenario responsible for a GlcN-dependent increase in LPS-mediated iNOS induction under 5 mM glucose conditions. The expression of iNOS must be tightly regulated by both transcriptional and post-transcriptional mechanisms. Post-transcriptional regulation of gene expression is often dependent on sequences located in the 3Ј-untranslated region (3Ј-UTR) of mRNAs (47,48). The zinc-finger protein TTP, KH-type splicing regulatory protein, and HuR all bind the iNOS mRNA 3Ј-UTR and recruits the exosome to the mRNA (49). LPS transiently up-regulated and GlcN inhibited such increases of the expression of TTP without affecting the other proteins, KH-type splicing regulatory protein or HuR under both 5 and 25 mM glucose conditions (data not shown). Therefore, up-regulation of TTP by LPS in our experimental model may contribute to an increased mRNA degradation. Although the present data suggest an involvement of this protein in iNOS expression, additional experiments are required to address the role of TTP in iNOS mRNA regulation under various glucose conditions.
The concentration of GlcN in the plasma of normal subjects is ϳ0.04 mmol/liter, rising up to 0.06 mmol/liter in those taking GlcN supplements (50). Intravenous administration of GlcN in rats induces much higher plasmatic concentrations than oral administration: infusion of 30.45 g of GlcN produced GlcN concentrations of ϳ1.42 mmol/liter (51). Because of the limited information regarding HBP flux, which is accelerated by GlcN infusion in humans, in vivo physiological dosing might be an important issue to be addressed in the future.
In summary, we have demonstrated that the combination of increased GlcN flux and nutrient availability from extracellular culture medium plays a distinct role in the induction of iNOS and potentially other proinflammatory mediators in response to LPS. This adds to the growing body of evidence stressing the importance of HBP as a nutrient-sensing pathway at both excess nutrient and normal physiology conditions. HBP may operate as a master regulator of inflammation signaling by governing the rapid switch from inflammatory activation to repression or vice versa. This master regulator depends on nutrient availability for inflammatory regulation by integrating transcriptional regulation and mRNA stability.

Experimental Procedures
Reagents-Reagents were purchased from Sigma or Amresco (Cochran Road, OH) unless otherwise noted.
Cell Cultures-RAW264.7 murine macrophage cells (purchased from the Korean Cell Line Bank) were maintained at 37°C at 5% CO 2 in DMEM supplemented with 5% FBS (Hyclone, Logan, UT), 1% streptomycin and penicillin. For peritoneal macrophage isolation, 6-week-old male BALB/c mice were intraperitoneally injected with 200 l of 3.5% thioglycollate, and cells were isolated from the peritoneal cavity 3 days after injection. The cells were then incubated for 6 h at 37°C and cells were washed twice with PBS. The remaining adherent cells were used as the peritoneal macrophages described in the experiments. Overnight cultured cells were washed twice with PBS and replaced DMEM containing 5 or 25 mM glucose. After 4 h, cells were treated with 5 mM GlcN for 2 h and then stimulated with 0.1 g/ml of LPS for 24 h.
Nitrite Assay-NO production was determined by measuring the amount of nitrite, as described with minor modifications (15). The 50 l of cultured medium was reacted with 50 l of Griess reagent (1% sulfanilamide, 0.1% naphthylenediamine, and 5% phosphoric acid). The optical density at 540 nm (OD 540 ) was measured with a microplate reader. Nitrite concentrations were calculated by comparison with observance of standard of NaN 2 solution.
Transient Transfection and Luciferase Assay-The NF-B reporter contained three copies of the NF-B binding site fused to the firefly luciferase gene (Clontech). Cells were transfected with Lipofectamine TM and PLUS TM reagents (Invitrogen) according to the manufacturer's protocol. After 3 h, medium was replaced with DMEM containing 5 or 25 mM glucose and stimulated with 0.1 g/ml of LPS in the presence or absence of 5 mM GlcN. Luciferase activity was measured using a Turner Designs luminometer (TD-20/20) at 24 h and normalized to transfection efficiency using a cotransfected ␤-galactosidase.
Streptavidin-Agarose Pulldown Assay-Streptavidin-agarose pulldown assays were performed as described previously (13). The procedure allows for quantitative binding of transcription factors or molecules of interest to a specific probe: a 20-nucleotide sequence containing the NF-B binding site (5Ј-GCTAGGGGGATTTTCCCTCT-3Ј) at position Ϫ957/Ϫ977 of the iNOS promoter. Total protein lysate was prepared with lysis buffer (10 mM HEPES, pH 8.0, 1.5 mM MgCl 2 , 200 mM sucrose, 0.5% Nonidet P-40, 10 mM KCl and protease inhibitors) and then nuclear proteins were extracted in lysis buffer (1 mM EDTA, pH 8.0, in PBS) with sonication. The nuclear protein samples were incubated with 2 g of biotinylated iNOS-B DNA probe and 25 l of steptavidin-conjugated agarose beads for 1 h at 4°C. DNA-protein complexes were determined by Western blotting using the indicated antibodies.