The anti-inflammatory activity of curcumin is mediated by its oxidative metabolites

The spice turmeric, with its active polyphenol curcumin, has been used as anti-inflammatory remedy in traditional Asian medicine for centuries. Many cellular targets of curcumin have been identified, but how such a wide range of targets can be affected by a single compound is unclear. Here, we identified curcumin as a pro-drug that requires oxidative activation into reactive metabolites to exert anti-inflammatory activities. Synthetic curcumin analogs that undergo oxidative transformation potently inhibited the pro-inflammatory transcription factor nuclear factor κB (NF-κB), whereas stable, non-oxidizable analogs were less active, with a correlation coefficient (R2) of IC50 versus log of autoxidation rate of 0.75. Inhibition of glutathione biosynthesis, which protects cells from reactive metabolites, increased the potency of curcumin and decreased the amount of curcumin-glutathione adducts in cells. Oxidative metabolites of curcumin adducted to and inhibited the inhibitor of NF-κB kinase subunit β (IKKβ), an activating kinase upstream of NF-κB. An unstable, alkynyl-tagged curcumin analog yielded abundant adducts with cellular protein that were decreased by pretreatment with curcumin or an unstable analog but not by a stable analog. Bioactivation of curcumin occurred readily in vitro, which may explain the wide range of cellular targets, but if bioactivation is insufficient in vivo, it may also help explain the inconclusive results in human studies with curcumin so far. We conclude that the paradigm of metabolic bioactivation uncovered here should be considered for the evaluation and design of clinical trials of curcumin and other polyphenols of medicinal interest.

tus with other dietary phenolic compounds, for example, resveratrol (from grapes and red wine), quercetin (onions), and epigallocatechin gallate (green tea) (3)(4)(5). Preclinical evidence from cell culture and animal models has provided incentive for testing dietary polyphenols in randomized clinical trials (6,7). In the form of an extract from the plant turmeric, curcumin has a rich history as an anti-inflammatory agent in traditional Asian medicine (8). Attempts to replicate these effects in clinical trials, however, have given mixed results, with therapeutic improvements limited to open-label studies (9,10).
A key element to improve design and outcome of clinical trials is a molecular understanding of how curcumin and related polyphenols affect their targets (11,12). Numerous cellular targets of curcumin have been identified but there is only limited insight into how such a wide range of targets can be affected by a single compound. The recognized molecular features of curcumin are its phenolic hydroxyl, mediating antioxidant activity (13), its ␣,␤-unsaturated enone, mediating protein binding (14), and its central ␤-dicarbonyl, mediating metal ion chelation (15). These features are not unique to curcumin and appear insufficient to account for the differences in bioactivity compared with other dietary polyphenols.
To explain the wide range of biological effects of curcumin we suggest a paradigm that takes into account the well recognized chemical instability of curcumin (16). Degradation of curcumin is initiated by H-abstraction from the phenolic hydroxyl, equivalent to its antioxidant reaction, and leads to rapid autoxidative transformation in buffer and cell culture medium (17). Oxidative degradation yields metabolites with reactive quinone methide, free radical, endoperoxide, and spiroepoxide moieties as intermediates in the transformation to the final bicyclopentadione product (18,19). Here we provide evidence that the electrophilic metabolites, rather than parent curcumin or the final bicyclopentadione, adduct covalently to cellular protein, and specifically to IKK␤ of the NF-B pathway to exert anti-inflammatory activity. This provides a chemical-molecular paradigm relating the structure of curcumin and its autoxidative/ antioxidant behavior to its anti-inflammatory activity.

Inhibition of NF-B correlates with oxidative transformation of curcumin
We synthesized analogs of curcumin that were designed to undergo or resist spontaneous autoxidation. Chemical stability was changed by blocking the phenolic hydroxyl through derivatization and modifying the methoxy groups at the aromatic rings. Based on the analysis of oxidative transformation of demethoxy-and bisdemethoxycurcumin (20) we had predicted that adding or removing methoxy groups from the aromatic rings would enhance or decrease, respectively, autoxidation. The rate of autoxidation of the compounds was quantified by monitoring the disappearance of the chromophore around 430 nm (Fig. 1A). Blocking one or both of the phenolic hydroxyl groups in compounds 2-4 increased the stability of the analogs. Adding a methoxy group in the meta-position of the aromatic ring increased autoxidation in 8 and 9 relative to 7 while decreasing autoxidation in 5 and 6 relative to curcumin. Autoxidation of analogs with a modified heptadienedione chain (10 -12) was also increased by placing meta-methoxy groups in the aromatic rings. Six of the compounds (5, 6, and 8 -11) were considered unstable, autoxidizing at rates slower or faster than curcumin. LC-MS analyses of autoxidation reactions of the unstable analogs showed the formation of products with an increase in molecular mass by 32 mass units, whereas the stable analogs did not form discernible dioxygenated products (Fig. S1). The increase by 32 mass units was consistent with the incorporation of two atoms of oxygen during the autoxidation, as is observed in the transformation of curcumin to bicyclopentadione (20,21).
The anti-inflammatory activity of curcumin and the synthetic analogs was tested in RAW264.7 cells stably expressing luciferase downstream of an NF-B response element. The dose-dependent inhibition of LPS-induced luciferase activity by the analogs was expressed as IC 50 values (Fig. 1A). Compounds 6, 8, and 9 had IC 50 values and rates of autoxidation comparable with curcumin. Compounds 10 and 11 inhibited NF-B at lower IC 50 than curcumin, which correlated with their increased rate of autoxidation. Stable analogs 4, 7, and 12

Oxidative activation of curcumin
were only weakly inhibitory such that their IC 50 values were estimated. Compounds 2 and 3 had inhibitory activity similar to curcumin although they did not readily autoxidize. Because both compounds have methyl groups placed at the phenolic hydroxyl we reasoned that these may have been removed enzymatically during the cell incubations, reverting the compounds back to curcumin. In fact, LC-MS analyses showed that both 2 and 3 had lost one or both para-methoxy groups, respectively, during cell incubations (Fig. S2). Metabolically formed curcumin was present in the cell supernatants at about 20 and 75% abundance relative to 2 and 3, respectively, and likely contributed to the inhibition of NF-B activity.
The HPLC-isolated spiroepoxide intermediate of curcumin autoxidation inhibited NF-B activation with an IC 50 of 29 M (Fig. 1B), demonstrating activity of an unstable and reactive intermediate. The technically challenging aspects of isolating a reaction intermediate, including loss during sample preparation and quantification of the unstable spiroepoxide, likely contributed to the increased IC 50 compared with curcumin. In addition, although at physiological pH the spiroepoxide has a half-life greater than curcumin it reacts much more readily with cellular thiols (19). Therefore, media and cellular environments likely contributed to scavenging of exogenously added spiroepoxide before it reached the inside of the cell and its protein targets. Nevertheless, the inhibitory potency of the spiroepoxide suggested that much of the anti-inflammatory activity of curcumin is mediated by this and other reactive intermediates of curcumin autoxidation. In contrast, the final bicyclopentadione oxidation product or the putative cleavage products, vanillin and ferulic acid (22)(23)(24), were unable or less able, respectively, to inhibit NF-B (Fig. 1B).

Inhibition of IKK␤ and p65
Both the upstream kinase IKK␤ as well as the p65 subunit of NF-B have been implicated as targets of the anti-inflammatory activity of curcumin (25,26). We used TNF␣-activated HeLa cells to determine targets of curcumin and its analogs within the NF-B pathway. Curcumin reduced phosphorylation of IKK␤ as well as p65 of the NF-B complex and inhibited proteasomal degradation of IB␣ ( Fig. 2) (27). These results were consistent with inhibition of IKK␤ (27). Oxidizable analog 8 had the same effect as curcumin, whereas nonoxidizable 4 did not inhibit phosphorylation of IKK␤ and p65 and did not inhibit degradation of IB␣ (Fig. 2), indicating that inhibition of NF-B activity was dependent on oxidative activation. This was further supported by inhibition of p65 phosphorylation by highly active and oxidizable 11 (Fig. 2).

Oxidative status of the cell determines the response to curcumin
We hypothesized that cellular GSH scavenges and inactivates curcumin-derived electrophiles. A corresponding putative curcumin-GS adduct was detected in the supernatants of RAW264.7 cells in LC-MS analyses (Fig. 3A) (28). Therefore, depletion of GSH was predicted to enhance the potency of curcumin. Stably transfected RAW264.7 cells were depleted of GSH by preincubation with BSO (buthionine sulfoximine), an inhibitor of GSH biosynthesis (29), resulting in significantly reduced levels of curcumin-GS adducts in the cells (Fig. 3B). BSO treatment caused a leftward shift of the dose-response curve for curcumin, indicating increased potency in the absence of GSH (Fig. 3C), compatible with higher concentrations of electrophilic curcumin metabolites.

Oxidative activation of curcumin
We next pre-treated RAW264.7 cells with N-acetylcysteine (NAC), 4 a GSH biosynthetic precursor (30). NAC shifted the dose-response curve for curcumin to the right, indicating a decrease in potency presumably due to increased protection of the cells from electrophilic oxidation products of curcumin (Fig. 3C).

Oxidative metabolites of curcumin adduct to IKK␤
We aimed to obtain direct evidence for the covalent adduction of curcumin-derived electrophiles to IKK␤ as a mechanism of inhibiting its kinase activity. We used a tryptic peptide of the activation loop of IKK␤ as a model to analyze covalent adduction to the redox-sensitive residue, Cys 179 (31). An equivalent but shorter peptide containing Cys 179 has been shown to mimic redox regulation of IKK␤ (32). Autoxidation of curcumin in the presence of the IKK␤-peptide gave adducted peptides with an increase of 400, 368, and 122 mass units, compatible with covalent adduction of oxidized curcumin (ϩ400), curcumin or its quinone methide (ϩ368), and a methoxyphenol cleavage product (ϩ122), respectively (Fig. 4A). Using hexadeuterated curcumin (with the label placed in the methoxy groups) the adducts showed an additional shift of 6 (ϩ406 and ϩ374) or 3 (ϩ125) mass units, respectively (Fig. S3). Incubation of HPLC-isolated spiroepoxide intermediate with the IKK␤-peptide gave abundant ϩ400 as well as ϩ122 adducts with no unadducted peptide remaining (Fig. 4B). In contrast, no adduct was detected when the IKK␤ peptide was incubated with bicyclopentadione or stable dimethylcurcumin 3, or when a C179A mutant peptide was used in the autoxidation of curcumin (Fig. 4, C and D, and Fig.  S3).

Oxidative metabolites of curcumin adduct to cellular protein
Alkynyl-tagged "click"-able derivatives have been used to analyze cellular protein targets of electrophilic small molecules (33,34). To analyze adduction of curcumin and its oxidative metabolites to cellular protein we designed analogs in which the aromatic methoxy group was modified to contain an alkynyl tag. We prepared stable and oxidizable alkynyl-tagged analogs of curcumin to analyze the role of oxidative activation in protein adduction. 3Ј-O-Alkynyl-5Ј-O-methoxycurcumin 8 was most similar to curcumin in autoxidation rate (1.6 Ϯ 0.4 M/min versus 4.0 Ϯ 0.4 M/min) and IC 50 for inhibition of NF-B (24 versus 18 M) (Fig. 1A). In contrast, alkynyl analog 7 was stable toward autoxidation and less active (IC 50 Ϸ 70 M).
RAW264.7 cells were treated with test compounds, and adducted proteins were detected after click reaction with azidelinked rhodamine and resolution using SDS-PAGE. Untagged curcumin showed a weak signal, which may have been due to its intrinsic fluorescence activity. Oxidizable alkynyl analog 8 adducted to cellular proteins of a wide molecular weight range in a dose-dependent manner (Fig. 5A). In contrast, when cells were incubated with stable 3Ј-O-alkynylcurcumin 7 rhodamine staining was less intense, indicating that protein adduction was significantly less abundant by a probe that was resistant to oxidative activation (Fig. 5B).
To validate whether protein adduction by 8 was representative of curcumin we pretreated cells with curcumin and stable or unstable analogs. Pretreatment with curcumin prior to oxidizable 8 significantly reduced staining, and this effect was even Oxidative activation of curcumin more prominent by pretreatment with more readily oxidizable 11 (Fig. 5C). In contrast, preincubation with autoxidation-resistant 4 did not reduce adduct formation. These results imply that covalent adduction of curcumin to cellular protein mainly occurred by its reactive metabolites and less by the parent compound.

Discussion
The chemical instability of curcumin in aqueous buffer is well recognized (17) and, in addition to its low bioavailability, widely considered a key factor that limits successful therapeutic application of curcumin (35,36). According to this hypothesis, loss of curcumin by degradation equals loss of the active compound (37). We propose the opposite. Our studies identify curcumin as a pro-drug that undergoes oxidative activation, i.e. degradation, into reactive metabolites as the mediators of its anti-inflammatory effects. The reactive metabolites, including quinone methide and spiroepoxide intermediates (19), cova-lently adduct to cellular protein thereby modulating function (Fig. 6).
One of the targets of the anti-inflammatory activity of curcumin is IKK␤ of the NF-B pathway. Covalent modification of Cys 179 of IKK␤ prevents phosphorylation of neighboring serines 177 and 181 and inhibits kinase activity (38). Cys 179 is the target of a number of lipid-derived small molecule electrophiles acting as endogenous anti-inflammatory mediators, including ⌬ 12,14 -15-deoxyprostaglandin J 2 , 4-hydroxy-nonenal, and nitro fatty acids (39 -41). Michael addition to IKK␤ as a mechanism for the anti-inflammatory activity has been attributed previously to the parent curcumin, and it was implied that the reacting electrophile is the ␣,␤-unsaturated enone (42). The enone in curcumin, however, is only a weak electrophile due to a conjugated double bond system that spans the entire molecule, as is evident from the yellow-orange color. Using the IKK␤ peptide probe we found that Cys 179 was adducted by the spiroepoxide and possibly other oxidative curcumin metabolites (ϩ400 atomic mass units) and a methoxyphenol cleavage product (ϩ122 atomic mass units), whereas the reacting species for the ϩ368 atomic mass unit adduct may be the parent curcumin or its quinone methide oxidative metabolite (Fig. 6). Whether the reaction occurs with curcumin or its quinone methide cannot readily be distinguished because the electrophilic carbon is identical in either molecule and thus predicted to result in the same adduct. The fact that two non-oxidizable analogs (3 and 4) containing the same ␣,␤-unsaturated enone as curcumin were unable to adduct to the peptide (Fig. S3)  To illustrate how oxidative activation of curcumin and the different analogs contributed to their anti-inflammatory activity, we plotted the IC 50 values for inhibition of NF-B versus the rate of autoxidation and observed a positive correlation (R 2 ϭ 0.75) (Fig. 7). The more readily a compound autoxidized to form a reactive electrophile the greater was its ability to inhibit NF-B. The relationship best fitted a hyperbolic curve, resulting in a straight line when the rate of autoxidation was plotted on a log scale. The non-linear relationship indicated that other factors contributed, including additional targets within the NF-B pathway, possible enzymatic activation of the com-

Oxidative activation of curcumin
pounds by peroxidases (21,43), as well as inhibitory effects independent of oxidative activation, conceivably due to the enone moiety present in 2-12. Last, the autoxidation rate is only a surrogate, albeit quantitative, measure for formation and reactivity of the electrophiles with protein thiols.
The bioactivation hypothesis implies that the potency of curcumin to inhibit NF-B is modulated by the redox status of the cell. The amount of GSH available to scavenge reactive electrophiles was inversely correlated to the activity of curcumin (Fig.  3). GSH protects cells from reactive metabolites of curcumin and limits their biologic effects that may also include oxidative damage to protein, membranes, and DNA. Inflammatory and cancer cells have an enhanced oxidative milieu (44), resulting in selective targeting by curcumin in a 2-fold manner. The oxidative milieu supports oxidative activation of curcumin, and the reduced levels of GSH weaken the ability of the cell to inactivate curcumin-derived electrophiles. Oxidative bioactivation is a novel paradigm to explain the cellular specificity of curcumin, which is known to target inflammatory as well as cancerous cells (45,46).
Oxidative bioactivation of curcumin resulted in the covalent modification of a large number of cellular proteins, undoubtedly contributing to the polypharmacology of curcumin. Hundreds of cellular proteins are regulated, at least in part, through oxidative modification of crucial cysteine residues (47,48), and these represent potential targets of bioactivated curcumin. An unstable, alkynyl-tagged probe mimicked protein binding by curcumin better than a stable probe (Fig. 5), although binding also by the parent curcumin could not be excluded. The alkynyl probes with differential stability developed here will be helpful in future studies to distinguish protein targets of oxidative metabolites and parent curcumin. Our results complement click chemistry approaches with the alkynyl tag placed at the phenolic hydroxyl of curcumin for the ease of chemical synthesis, and thus relying on the ␣,␤-unsaturated enone as the sole electrophile (49 -51). Based on our findings, these probes are predicted to have less anti-inflammatory and likely less overall biological activity. They are suited to detect protein targets of the parent curcumin but not of its reactive metabolites.
The biological and antioxidant activities of some of the compounds tested here (3, 6, and 10 -12) have been evaluated previously in prototypical anti-inflammation assays (52,53). In one study, 7 of 8 curcumin analogs that cannot oxidize due to blocking or absence of a phenolic hydroxyl were inactive in reducing rat paw edema, whereas 6 analogs that autoxidize or can be predicted to autoxidize showed activity comparable with curcumin (52,54). There was a similar correlation for three oxidizable curcumin analogs (6, 10, and 11) in the transcriptional inhibition of cyclooxygenase-2 and inducible nitric-oxide synthase as well as inhibition of phorbol ester-induced ornithine decarboxylase activity in mouse skin (53,54). Although metabolic events were not considered, these studies support the hypothesis of oxidative activation as a key mechanism mediating anti-inflammatory activities of curcumin.
There has been much interest in identifying metabolites as the active principle of dietary polyphenols and flavonoids in recent years (55,56). The majority of investigations has focused on stable metabolites that can be detected in plasma and urine or degradation products generated via microbial transformation by intestinal bacteria (57,58). In the case of curcumin, vanillin and ferulic acid have been invoked as potentially bioactive metabolites (22,23). In contrast to these approaches, we have identified short-lived reactive metabolites as the main molecular species mediating anti-inflammatory effects and protein adduction of curcumin. This aligns with the concept of "biological reactive intermediates" generated from botanicals that mediate beneficial but also toxicological effects (59,60). On a cautionary note, our studies have been conducted in cultured cells in vitro where oxidation of curcumin occurs readily. The extent of oxidative activation of curcumin in vivo is unknown. The known metabolism of curcumin in vivo entails reduction and/or conjugation with glucuronic acid and sulfate (61). Our attempts to provide evidence for oxidative transformation of curcumin in vivo, focusing on the detection of bicyclopentadione formation, have not been successful so far. 5 In hindsight, this may not be surprising, considering the ready adduction of reactive intermediates to cellular thiols, resulting in metabolic redirection away from the final bicyclopentadione toward adduct formation. Nevertheless, our studies provide evidence that reactive metabolites from polyphenols are mediators of biological effects. A detailed understanding of the chemical reaction steps from the starting polyphenol to the detectable metabolites (19) will help in the identification of intermediate bioactive metabolites of other polyphenols and flavonoids of high medicinal interest.
Extracts of the plant turmeric, with its active polyphenol curcumin, have been used as an anti-inflammatory remedy in traditional Asian medicine for centuries. The widespread hopes for curcumin as a natural agent for the treatment of inflammatory and cancerous diseases have been fueled by positive results in numerous cell culture-based assays and animal models. Bioactivation occurs readily in vitro, which can explain the wide range of cellular targets of curcumin identified whereas their abundance bears the risk of overestimating its therapeutic potential. In turn, insufficient bioactivation in vivo may underlie the disappointing results in human studies with curcumin so far. Humans that are healthy and exhibit low levels of oxidative 5 P. B. Luis and C. Schneider, unpublished data.

Oxidative activation of curcumin
stress may be less likely to experience the benefits resulting from oxidative activation of curcumin but also be protected from potentially harmful effects. A judgment of the therapeutic potential of curcumin will be more adequate if the role of bioactivation is considered for the interpretation of in vitro data as well as for the selection of in vivo targets. The paradigm of metabolic bioactivation is likely to extent to other dietary polyphenols and flavonoids and should be considered for the evaluation and design of clinical trials.

Materials
Stock solutions of curcumin and analogs were prepared at 5 mM in methanol or at 10 mM in DMSO and stored at Ϫ20°C. Lipopolysaccharide (LPS) from Escherichia coli serotype 0111:B4 was obtained from Sigma. HeLa and RAW264.7 cells were from ATCC.

Synthesis of curcumin and compounds 2-6
Curcumin and compounds 2-6 were synthesized following the general scheme of adding two aromatic aldehyde moieties to acetylacetone according to Pabon (62) using the appropriate aldehydes as described in Ref. 63.

Synthesis of compounds 10 -12
Compounds 10-12 were synthesized according to Ref. 63 using the appropriate cyclohexanone or cyclopentanone and aldehyde starting materials.

Autoxidation of curcumin and analogs
Autoxidation rates of curcumin and its analogs were determined using a UV-visible spectrophotometer operated in the time-drive mode. Compounds were added at 50 M to 500 l of 20 mM ammonium acetate buffer, pH 7.5, and disappearance of the maximum absorbance for each compound (which was around 425 nm) was followed for 20 min. The rate of autoxidation was calculated using the part of the curve that showed the fastest decrease.

NF-B luciferase reporter assay
A Ready-to-glow Secreted Luciferase Reporter assay was used to quantify the effects of compounds on LPS-induced Oxidative activation of curcumin NF-B activity. Stably transfected RAW267.4 cells were seeded in a 24-well plate at a concentration of 250,000 cells/well in 500 l of DMEM. For some experiments, cells were incubated with N-acetyl-cysteine (3 mM) or buthionine sulfoximine (BSO; 0.5 mM) overnight. Cells were washed twice with cold PBS and pre-treated with curcumin or analogs (0 -100 M) in DMEM for 45 min. The cells were then stimulated with LPS (100 ng/ml). After 4 h, 50 l of the medium was removed and luciferase activity was determined.

LC-MS analysis of autoxidation products
Curcumin and analogs (50 M) were incubated in 20 mM ammonium acetate buffer, pH 7.5, for 20 min. Samples were extracted using Waters HLB cartridges and dissolved in 50 l of acetonitrile/water (1:1) for LC-MS analysis. Samples were electrosprayed into a Thermo Vantage triple quadrupole mass spectrometer operated in the negative ion mode and coupled to a Waters Acquity UPLC system with a Waters symmetry Shield C18 3.5-m column (2.1 ϫ 100 mm) using a gradient of 5 to 95% acetonitrile in water, 0.1% formic acid within 3 min, and a flow rate of 0.4 l/min.

LC-MS analysis of curcumin-glutathione adducts
Media samples of RAW264.7 cells treated with vehicle or BSO and curcumin were extracted using Waters HLB cartridges eluted with methanol. LC-MS analyses were performed in the negative ion mode. The same MS and UPLC system as for product analysis was used. The column was eluted with a gradient of acetonitrile in water, 0.1% formic acid changed from 30% acetonitrile to 60% in 2 min and further increased to 95% in 2 min at a flow rate of 0.4 ml/min. The selected reactionmonitoring transitions were for curcumin m/z 367 3 217, d 6 -curcumin m/z 373 3 220, and for curcumin-GSH adduct m/z 674 3 360.

LC-MS analysis of IKK␤ peptide adducts
In silico tryptic digestion of IKK␤ gave a peptide ( 172 ELDQG-SLCTSFVGTLQYLAPELLEQQK 198 ) that contained Cys 179 (underlined) (M r 3012.43 g/mol). 10 M Cys 179 (or Ala 179 ; M r 2980.36 g/mol) IKK␤ peptide was incubated with curcumin or analog (50 M) at 37°C in acetate buffer, pH 7.5, for 1 h. Aliquots were analyzed directly using a Waters Synapt G1 TOF instrument in positive ionization mode following separation of the sample using a Waters XSelect C18 column with a gradient of acetonitrile/water, 15:85 (by volume) to 65:35 (by volume), containing 0.1% formic acid within 10 min at a flow rate of 0.25 ml/min.

Detection of alkynyl-tagged curcumin-cellular protein adducts
RAW267.4 cells were seeded at 500,000 cells/ml in 6-well plates and allowed to adhere overnight. The cells were preincubated with DMSO, curcumin, or analogs (150 M each) for 2 h, followed by incubation with 8 for 2 h. Cells were lysed using 100 mM sodium phosphate buffer, pH 7.5, with 0.1% Triton X-100 followed by centrifugation at 13,000 ϫ g at 5°C for 10 min. For click chemistry 50 g of protein was incubated with rhodamine-B-azide (20 M; Azide Fluor 545, Sigma), Tris(2-carboxyethyl)phosphine hydrochloride (1 mM), Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine (100 M), and CuSO 4 (1 mM) for 2 h. Excess reagents were removed by acetone precipitation at Ϫ20°C overnight. Samples were centrifuged at 13,000 ϫ g for 10 min, acetone was removed, and samples were left to air dry for 30 min. 25 g of protein sample solubilized in loading buffer were resolved on a 4 -20% SDS-PAGE gel. Protein adducts were visualized by scanning the gel using the Gel Doc EZ system (Bio-Rad) with settings for detection of ethidium bromide. The gel was then washed three times with water and stained with Coomassie Blue.