The Sec61/SecY complex is inherently deficient in translocating intrinsically disordered proteins

About one-quarter to nearly one-third of the proteins synthesized in the cytosol of eukaryotic cells are integrated into the plasma membrane or are secreted. Translocation of secretory proteins into the lumen of the endoplasmic reticulum or the periplasm of bacteria is mediated by a highly conserved heterotrimeric membrane protein complex denoted Sec61 in eukaryotes and SecYEG in bacteria. To evaluate a possible modulation of the translocation efficiency by secondary structures of the nascent peptide chain, we performed a comparative analysis in bacteria, yeast, and mammalian cells. Strikingly, neither the bacterial SecY nor the eukaryotic Sec61 translocon was able to efficiently transport proteins entirely composed of intrinsically disordered domains (IDDs) or β-strands. However, translocation could be restored by α-helical domains in a position- and organism-dependent manner. In bacteria, we found that the α-helical domains have to precede the IDD or β-strands, whereas in mammalian cells, C-terminally located α-helical domains are sufficient to promote translocation. Our study reveals an evolutionarily conserved deficiency of the Sec61/SecY complex to translocate IDDs and β-strands in the absence of α-helical domains. Moreover, our results may suggest that adaptive pathways co-evolved with the expansion of IDDs in the proteome of eukaryotic cells to increase the transport capacity of the Sec61 translocon.

About one-quarter to nearly one-third of the proteins synthesized in the cytosol of eukaryotic cells are integrated into the plasma membrane or are secreted. Translocation of secretory proteins into the lumen of the endoplasmic reticulum or the periplasm of bacteria is mediated by a highly conserved heterotrimeric membrane protein complex denoted Sec61 in eukaryotes and SecYEG in bacteria. To evaluate a possible modulation of the translocation efficiency by secondary structures of the nascent peptide chain, we performed a comparative analysis in bacteria, yeast, and mammalian cells. Strikingly, neither the bacterial SecY nor the eukaryotic Sec61 translocon was able to efficiently transport proteins entirely composed of intrinsically disordered domains (IDDs) or ␤-strands. However, translocation could be restored by ␣-helical domains in a position-and organism-dependent manner. In bacteria, we found that the ␣-helical domains have to precede the IDD or ␤-strands, whereas in mammalian cells, C-terminally located ␣-helical domains are sufficient to promote translocation. Our study reveals an evolutionarily conserved deficiency of the Sec61/ SecY complex to translocate IDDs and ␤-strands in the absence of ␣-helical domains. Moreover, our results may suggest that adaptive pathways co-evolved with the expansion of IDDs in the proteome of eukaryotic cells to increase the transport capacity of the Sec61 translocon.
Around 25-30% of all proteins synthesized in the cytosol of eukaryotic cells are integrated into the plasma membrane or secreted. Membrane integration and import into the lumen of the endoplasmic reticulum (ER) 3 is mainly mediated by a het-erotrimeric membrane protein complex called the Sec61 complex, which is highly homologous to the SecY translocon in bacteria (reviewed in Refs. [1][2][3][4][5]. A major fraction of secretory proteins contains N-terminal signal peptides, which mediate transport of the nascent chain to the Sec61 translocon and initiate translocation into the ER lumen (reviewed in Refs. 3 and 6 -13). Failure to deliver secretory or membrane proteins to the ER can have fatal consequences. First, secretory proteins do not reach their final destination (loss of function), and second, these proteins may form cytosolic aggregates with toxic activities (gain of toxic function) because they often contain hydrophobic domains. For example, mislocalization of the cellular prion protein to the cytosol can induce neuronal cell death in mammalian cell culture models and transgenic mice (14 -17). Thus, quality control pathways have evolved to rapidly eliminate mistargeted proteins (18 -21).
Each secretory protein has an individual signal sequence, although the targeting peptide is usually removed during transmembrane transport and therefore is not part of the mature protein. However, there are differences in ER import efficiency due to the variability of ER signal sequences (reviewed in Refs. [22][23][24][25]. Context-specific regulation of protein translocation might enable the cell to prevent an overload of protein folding and quality control pathways in the ER lumen (26 -28). In addition, it might allow for dual targeting of secretory proteins to other cellular compartments to expand their physiological function (29,30).
ER signal peptides have an intrinsic activity to mediate ER import. For example, they can be fused to heterologous nonsecretory proteins to mediate their ER import. However, we and others demonstrated that intrinsically disordered proteins (IDPs) equipped with authentic N-terminal ER signal peptides failed to be productively imported into the ER lumen of neuronal cells (31)(32)(33)(34)(35). This might be related to the lack of structural elements, which have been shown to already form within the ribosomal tunnel and to modulate the translocation process (36 -46). We now present evidence for a deficiency of the Sec61/SecY complex of eukaryotes and bacteria to translocate substrates that are entirely unstructured or composed only of ␤-strands. Moreover, our study may indicate the evolution of auxiliary translocon components in eukaryotes to expand the transport capacity of the Sec61 complex for secretory proteins with extended unstructured domains.

The Sec61 and SecY complex cannot efficiently translocate intrinsically disordered proteins
Previous studies revealed that productive ER import of IDPs equipped with authentic N-terminal ER signal peptides is greatly impaired in mammalian cells (31,32,34,35). Because the Sec61 complex in eukaryotes is highly homologous to the SecY complex in bacteria (reviewed in Refs. [1][2][3][4], the question emerged of whether impaired translocation of IDPs is a general phenomenon of Sec61/SecY-mediated translocation. To address this question experimentally, we studied transport of IDPs through the Sec61 complex in mammalian and yeast cells and the SecY complex in Escherichia coli. We started with two sets of model substrates that are either composed of ␣-helical domains or entirely intrinsically disordered. These model substrates were equipped with authentic N-terminal signal peptides of the respective organisms. Mammalian constructs contain the signal peptide of mouse PrP, and for secretion in E. coli, the substrates contained the PelB or DsbA signal peptide to target the proteins via either the SecA (mainly post-translational) (47) or SRP-dependent pathway (mainly co-translational) (48) to the SecY complex. The sequences of all constructs used in this study are shown in Table S1. For the analysis of eukaryotic cells, all constructs contained acceptor sites for N-linked glycosylation to monitor productive import into the ER by the appearance of a glycosylated protein fraction (Fig.  1A). Transport of the constructs into the periplasm was analyzed by separating transformed bacteria into periplasmic and cytoplasmic fractions and subsequent Western blotting (Fig.  1B). To monitor the purity of the periplasmic fraction, the cytosolic chaperonin GroEL and the periplasmic maltose-binding protein (MBP) were analyzed in parallel.
To validate our experimental strategy, we first analyzed Sec61-and SecY-mediated translocation of the highly structured C-terminal domain (aa 122-254) of the mammalian prion protein (49 -51) (Fig. 1C, top). In this context, it is important to note that in vitro experiments indicated that even a PrP fragment comprising only helix 2 and helix 3 shows complete structural autonomy (i.e. it independently adopts an ␣-helical conformation) (52). Corroborating previous results (31), the ␣-helical model substrate was modified in HeLa cells with N-linked glycans (i.e. imported into the ER), because it was sensitive to digestion with peptide:N-glycosidase F (PNGase F). The multiple glycosylation bands result from the formation of glycans of complex structure, specifically seen for membraneanchored PrP constructs (53). After transient inhibition of the proteasome with MG-132, we did not observe stabilization of unglycosylated species, which would be indicative of an incomplete ER import of the ␣-helical substrate (Fig. 1C, left). Simi-larly, the ␣-helical protein was transported through the SecY complex in E. coli. Both the DsbA and PelB signal peptides were proficient in mediating secretion into the periplasm (Fig. 1C, right). Note that some MBP was always found in the cytosolic fraction due to incomplete permeabilization of the outer membrane. Importantly, GroEL was absent in the periplasmic fraction, indicating the purity of this fraction.
A highly conserved feature of PrP is a polybasic motif (KKRPK) located directly after the signal peptide cleavage site (aa 22 in human or mouse PrP). Because charged residues in close proximity to the signal peptide cleavage site may affect translocation (54 -56), we were wondering whether such a motif would modulate the translocation efficiency of our substrates. Thus, we inserted the polybasic motif directly after the signal peptide and analyzed secretion. Interestingly, in E. coli, the polybasic motif abrogated translocation (Fig. 1D, right), whereas it had no obvious effect on the translocation into the ER of HeLa cells (Fig. 1D, left).
Next, we analyzed Sec61/SecY-mediated translocation of substrates that are entirely unstructured. We used two model substrates that are either 133 aa (IDD133; Fig. 2A) or 209 aa (IDD209; Fig. 2B) in length. In addition, we generated two versions of each construct, one containing the polybasic motif and the other containing a mutated version to eliminate the positively charged residues (AAAPA). In line with previous studies (31,32,35), all unstructured constructs were not imported into the ER of mammalian cells, because we could not detect any glycosylated variants (Fig. 2, A and B, left). The shorter constructs could only be detected after proteasomal inhibition ( Fig.  2A, left, ϩ MG-132). The longer constructs were more stable, but the endoglycosidase H (Endo H) digestion revealed that these proteins were not glycosylated (Fig. 2B, left, ϩ EndoH). Similarly to the findings in mammalian cells, secretion of the intrinsically disordered proteins was greatly impaired in E. coli. All proteins were detectable in the cytosol of transformed E. coli, indicating that impaired secretion was not due to lack of expression or rapid degradation in the cytosol (Fig. 2, A and B , right). Interestingly, cleavage of the signal peptide seems to be more efficient in the KKRPK IDD209 constructs (Fig. 2B, right).
In HeLa cells, the unstructured proteins could be stabilized by MG-132, revealing that they were subjected to proteasomal degradation in the cytosol. The proteasome is part of a quality control system designated ER-associated degradation (ERAD), which mediates post-translational degradation of non-native ER proteins after their retrotranslocation back into the cytosol (57)(58)(59). ERAD substrates are usually characterized by a cleaved N-terminal signal peptide. To analyze whether this was the case for IDD209, we generated a mutant lacking the N-terminal ER signal peptide (IDD209⌬SP). IDD209 migrated more slowly on SDS-PAGE than IDD209⌬SP, suggesting that IDD209 contained an uncleaved N-terminal ER signal peptide and had not been imported into the ER lumen (Fig. 2B, bottom  left). Thus, degradation of IDD209 by the proteasome is obviously not linked to ERAD. To test for the possibility that heterologous intrinsically disordered domains could restore ER import of the unstructured domain of PrP, we fused the intrinsically disordered domains of Tau (Tau40/P301L aa 103-197) or ␣-synuclein (aa 2-114) to an IDD derived from PrP (IDD114⅐Tau and  (1). Proteins containing ␣-helical domains are translocated through the Sec61 translocon. During translocation, the signal peptide is cleaved, and N-linked glycans are added (2). Intrinsically disordered proteins are not imported into the ER and remain unmodified in the cytosol. Right, schematic diagram of a Western blot analysis. Upon Endo H or PNGase F treatment, the glycans of proteins imported into the ER (1) are cleaved off, leading to an increased electrophoretic mobility during SDS-PAGE (3). Proteins that have not been imported into the ER contain an uncleaved signal peptide, are unglycosylated (2), and migrate slightly more slowly than unglycosylated proteins after retrotranslocation from the ER. B, SecY-mediated translocation in E. coli. Left, schematic diagram to illustrate the subcellular localization of the analyzed substrates (1). Proteins dominated by ␣-helical domains are translocated through the SecY translocon into the periplasm. Upon translocation, the signal peptide is cleaved (2). Intrinsically disordered proteins are not imported into the periplasm and stay in the cytosol. Respective fractions were separated, and proteins were detected afterward by Western blotting (right). C and D, top, schematic presentation of the constructs analyzed. Dark rectangle, signal peptide; ␣-helical structure derived from PrP is indicated by helices; polygons, N-linked glycosylation acceptor site; gray rectangle, GPI anchor signal sequence. The total length of the proteins is indicated. C, ␣-helical proteins are efficiently translocated through the Sec61 complex in mammalian cells and the SecY complex in E. coli. Left, cell lysates of transiently transfected HeLa cells were analyzed by Western blotting. One set of cells was cultivated in the presence of the proteasomal inhibitor MG-132 (30 M, 3 h). To analyze N-linked glycosylation, lysates were treated with PNGase F (ϩ) before Western blotting. White arrowhead, unglycosylated protein fraction; black arrowhead, glycosylated protein fraction. Right, transformed E. coli expressing the protein either with the DsbA or PelB signal peptide were fractionated, and the cytoplasmic (C) and periplasmic (P) fractions were analyzed by Western blotting. Unprocessed full-length constructs (dots) and constructs after signal peptide cleavage (asterisks) are marked. The cytosolic chaperonin GroEL and the periplasmic MBP were analyzed in parallel. Translocation efficiency is indicated. Data represent mean Ϯ S.E. of at least three independent experiments. D, charged residues interfere with translocation in E. coli, but not in mammalian cells. The naturally occurring polybasic motif (amino acid sequence KKRPK) of PrP C was introduced next to the signal peptide. Expression and translocation in HeLa cells (left) and E. coli (right) was analyzed as described in C.
Because the translocation efficiency in mammalian cells can be modulated in a signal peptide sequence-specific manner (22)(23)(24)(25), we equipped the unstructured protein with the ER signal peptide of rat growth hormone (GH⅐209), which has previously been shown to be more efficient in promoting translocation than the PrP signal peptide (31, 60). However, produc- To monitor ER import, lysates were treated with Endo H (ϩ) before Western blotting. White arrowhead, unglycosylated protein fraction. To analyze processing by the signal peptidase in HeLa cells, expression of a mutant lacking the ER signal peptide (⌬SP) was analyzed in parallel. Right, transformed E. coli expressing the protein either with the DsbA or PelB signal peptide were fractionated, and the cytoplasmic (C) and periplasmic (P) fraction was analyzed by Western blotting. Unprocessed full-length constructs (dots) and constructs after signal peptide cleavage (asterisks) are marked. The cytosolic chaperonin GroEL and the periplasmic MBP were analyzed in parallel. Translocation efficiency is indicated. Data represent mean Ϯ S.E. of at least three independent experiments. C, heterologous intrinsically disordered domains do not restore ER import. To study intrinsically disordered domains with different primary structure, we fused the intrinsically disordered domain of Tau (aa 103-197) and ␣-synuclein (aa 2-140) to the IDD114 derived from PrP. To monitor ER import, lysates were treated with EndoH (ϩ) before Western blotting. White arrowhead, unglycosylated protein fraction. D, intrinsically disordered proteins are not imported into the ER of S. cerevisiae. Expression of three intrinsically disordered proteins derived from PrP S. cerevisiae was analyzed by Western blotting of total cell lysates after treatment with Endo H as described for HeLa cells in A.

Impaired Sec61/Y-mediated translocation
tive ER import of the fully unstructured protein in HeLa cells was not restored (data not shown).
To study Sec61-mediated translocation in Saccharomyces cerevisiae, the unstructured model substrates were equipped with the ER signal peptide of the yeast protein Kre5p, which mediates co-translational targeting to the ER. Cell lysates of transformants were prepared and analyzed by Western blotting. In line with the findings in mammalian cells, the electrophoretic mobility of the three different intrinsically disordered proteins was not changed after Endo H digestion, indicating that they have not been imported into the ER of S. cerevisiae (Fig. 2D).
In summary, our studies in bacteria, yeast, and mammalian cells revealed a conserved impairment of the Sec61/SecY complex to translocate substrates that are entirely intrinsically disordered. Aborted translocation seems to be independent of the length of the constructs, the efficiency of the signal peptide, and the targeting mode.

Selective impairment of the SecY complex in E. coli to translocate substrates with extended unstructured N-terminal domains
To test whether translocation of an unstructured domain can be restored, we fused ␣-helical domains to the C terminus of an unstructured protein. Indeed, Endo H digestion revealed that in HeLa cells, ER import of the unstructured domain was restored by the ␣-helical domains (Fig. 3A, HeLa, black arrowhead). Note that these constructs are modified only with high-mannose glycans but are secreted (data not shown). The faster migrating bands represent unglycosylated molecules (white arrowhead) that have failed to be imported into the ER. Consequently, this fraction is stabilized in cells incubated with the proteasomal inhibitor MG-132 (data not shown). The same phenomenon was observed in our yeast model. The Western blot analysis of cell lysates prepared from transformed S. cerevisiae showed two prominent bands. After Endo H digestion, the upper band disappeared, indicating that a fraction of the substrate with an extended unstructured domain was imported into the ER (Fig. 3A, S. cerevisiae). In contrast, a C-terminally located ␣-helical domain was not able to promote SecY-mediated translocation of an extended intrinsically disordered domain in E. coli. Secretion was not improved by using a signal peptide for co-translational targeting, as neither the DsbA nor the PelB signal peptide allowed translocation of this construct into the periplasm (Fig. 3A, bottom, E. coli).
To address the possibility that the localization of the N-glycosylation site within a construct affects the glycosylation efficiency, we analyzed glycosylation of IDD⅐␣ constructs with the N-glycosylation site located either at aa 31 (31CHO) or at aa 124 and 140 (124/140CHO) (Fig. 3B). However, the position of the N-glycosylation site has no significant influence on the glycosylation efficiency (Fig. 3B, 124/140CHO versus 31CHO). Based on the impaired secretion of the artificial IDD⅐␣ constructs in E. coli, we analyzed secretion of two naturally occurring mammalian proteins in E. coli that are characterized by extended unstructured domains: wild-type prion protein (49, 51) and Shadoo. Shadoo is a highly conserved neuronal glycoprotein present in all vertebrates with a stress-protective activity (35,61,62). Of note, full-length PrP is efficiently imported into the ER of mammalian (Fig. 4A, left) and yeast cells (63,64). In contrast, wild-type PrP equipped with the DsbA or PelB signal peptide was not secreted in E. coli, not even in the absence of the positively charged residues next to the signal peptide (Fig. 4A,  right). Similarly, full-length Shadoo, a protein that is efficiently imported into the ER of HeLa cells, is not secreted into the periplasm of bacteria (Fig. 4B).
To address the possibility that the localization of an unstructured domain within the protein has an effect on the translocation efficiency, we cloned the domains in reversed order (i.e. the ␣-helical domains were placed directly after the signal peptide followed by a C-terminally unstructured domain). In mammalian cells, import efficiency was increased by positioning the ␣-helical domain N-terminal to the unstructured domain. Moreover, this construct was also secreted in E. coli, revealing that extended unstructured domains can be transported through the SecY complex if they are preceded by an ␣-helical domain (Fig. 5A). However, compared with mammalian cells, the translocation of the ␣⅐IDD construct in E. coli was less efficient. Next, we evaluated the role of the position of the ␣-helical domain within an intrinsically disordered protein in more detail. To this end, we placed the ␣-helical domains at three different positions into a long unstructured protein: either at the C or N termini or in the middle (Fig. 5B). Remarkably, the analysis in HeLa cells revealed that ␣-helical domains located 187 aa downstream to the signal peptide are still able to restore ER import of an intrinsically disordered domain (Fig. 5B). To further study impaired translocation of proteins with an unstructured N-terminal domain in E. coli, we employed an in vitro system for SecY-mediated translocation (65). As expected, the ␣-helical protein was imported into SecY-containing inverted inner membrane vesicles (INVs) (Fig. 5C, ␣), as indicated by signal sequence cleavage and proteinase K protection. A typical feature of bacterial in vitro systems is that protease K protection is also observed for the non-processed substrate in the presence of INVs. This is related to the low activity of the signal peptidase in the isolated INVs (65,66). An additional band of low molecular weight ( Fig. 5C (#)) was present after proteinase K treatment in the absence of INVs, which most likely corresponds to partially protease-resistant aggregates, but this was not further analyzed. Consistent with our in vivo results observed in E. coli, SecY-mediated translocation in vitro was also significantly impaired for both the completely unstructured protein (IDD) and the construct with an extended N-terminal unstructured domain (IDD⅐␣) (Fig. 5C). For both constructs, only background protease protection was observed. It should be noted that complete membrane-free in vitro systems are almost impossible to generate, as the sucrose-gradient purified ribosomes usually contain some minor membrane contamination (65). This explains the weak protease resistance even in the absence of added INVs. Interestingly, cleavage of the signal peptide seemed to occur for all three proteins, suggesting that the secondary structure did not impair targeting and the initial insertion of the proteins into the translocon but rather their productive translocation. Processing without translocation has been observed before for native E. coli secretory proteins when Impaired Sec61/Y-mediated translocation the translocation activity of the SecYEG translocon is impaired (e.g. by deleting the SecG subunit of the SecYEG translocon) (66). The notion that targeting of the IDDs to the translocon occurs is in line with previous in vitro studies employing stalled ribosome-nascent chain complexes and canine pancreatic microsomal membranes (34).
These findings indicate that ␣-helical domains are required in addition to N-terminal signal peptides to mediate translocation of unstructured domains through the Sec61 or SecY translocon. The ␣-helical domains must be close to the signal peptide in the case of SecY-mediated translocation in E. coli. In eukaryotic cells, ␣-helical domains promote translocation even if they are located To analyze ER import in eukaryotic cells, lysates were treated with Endo H (ϩ) before Western blotting. White arrowhead, unglycosylated protein fraction; black arrowhead, glycosylated protein fraction. Right, the percentage of the ER-translocated fraction was determined as the ratio between the signal of the glycosylated fractions and the total protein signal. Data represent mean Ϯ S.E. (error bars) of three independent experiments. ns, not significant. Bottom, transformed E. coli expressing the proteins either with the DsbA or PelB signal peptide were fractionated, and the cytoplasmic (C) and periplasmic (P) fractions were analyzed by Western blotting. Unprocessed full-length constructs (dots) and constructs after signal peptide cleavage (asterisks) are marked. The cytosolic chaperonin GroEL and the periplasmic maltose-binding protein (MBP) were analyzed in parallel. Translocation efficiency is indicated. Data represent mean Ϯ S.E. of at least three independent experiments. B, the position of the N-glycosylation site does not modulate glycosylation efficiency. Top, schematic presentation of the constructs analyzed. Dark rectangle, signal peptide; helices, ␣-helical structure derived from PrP; straight line, intrinsic disorder derived from PrP. The N-glycosylation sites (polygons) are either at aa 31 (31CHO) or at aa 124 and 140 (124/140CHO). The total length of the proteins is indicated. Bottom left, the indicated substrates, containing the glycosylation site at different positions, were expressed in transiently transfected HeLa cells. Cell lysates were treated with Endo H (ϩ) before Western blotting to monitor ER import. White arrowhead, unglycosylated protein fraction; black arrowhead, glycosylated protein fraction. Bottom right, the percentage of the ER-translocated fraction was determined as the ratio between the signal of the glycosylated fractions and the total protein signal. Data represent mean Ϯ S.E. of three independent experiments. ns, not significant.

Impaired Sec61/Y-mediated translocation
more than 180 aa distal to the signal peptide, although translocation efficiency decreases with increasing distance.

␤-Strand proteins are poor substrates for Sec61-and SecY-mediated translocation
In a next step, we extended our analysis to assess a possible impact of ␤-strands on ER translocation. We therefore fused a domain composed of ␤-strands C-terminally to the unstructured domain (IDD⅐␤) and compared ER import efficiency to an IDD⅐␣ protein in mammalian cells. Remarkably, the ␤-strands were markedly less potent in restoring ER import of the IDD compared with ␣-helical domains (Fig. 6A, IDD⅐␣ versus IDD⅐␤). Located C-terminally to ␣-helical domains, neither ␤-strands nor an unstructured domain significantly impaired ER import (Fig. 6A, ␣⅐IDD versus ␣⅐␤). To follow up on this observation, we analyzed ER import of two substrates that are dominated by either ␣-helical domains or ␤-strands without discernible ␣-helical domains. Indeed, in eukaryotic cells, ER import efficiency of a protein composed only of ␤-strands was significantly decreased compared with an ␣-helical protein (Fig. 6B, ␣ versus ␤). Similarly, SecY-mediated translocation of the ␤-strand protein in E. coli was also reduced (bottom) and only seen when the ␤-strand protein was equipped with a signal peptide for a SecA-dependent targeting (PelB) (Fig. 6B, bottom  right). These findings are in line with the observation that ␤-barrel proteins in E. coli are targeted via the SecA-dependent pathway to the SecY complex. Moreover, when we inspected the secondary structure of the outer membrane porins OmpC, OmpE, and OmpF, we noticed that these proteins contain several short ␣-helical domains in addition to the ␤-strands (Uni-ProtKB P06996, P02932, and P02931).

Discussion
In all forms of life, protein secretion is mediated by a highly conserved complex designated Sec61 in eukaryotes and SecY in prokaryotes and archaea (reviewed in Refs. [1][2][3][4][5]. In this study, we show that the Sec61/SecY complex is designed for the translocation of client proteins with ␣-helical structures. Even if equipped with highly potent signal peptides, proteins with ␤-strands are only less efficiently translocated, whereas intrinsically disordered proteins cannot be productively translocated at all. As a consequence, ␣-helical domains are required in addition to the N-terminal signal peptide to ensure effective translocation. Furthermore, our study reveals adaptive pathways to enable the transport of substrates with extended N-terminal unstructured domains through the Sec61 translocon in eukaryotic cells.

Impaired translocation of intrinsically disordered and ␤strand domains is an evolutionarily conserved feature
In addition to their role to target substrates and initiate transport through the translocon, signal peptides can modulate translocation efficiency in a sequence-and context-specific To analyze ER import in eukaryotic cells, lysates were treated with PNGase F (ϩ) before Western blotting. White arrowhead, unglycosylated protein fraction; black arrowhead, glycosylated protein fraction. Transformed E. coli expressing the proteins either with the DsbA or PelB signal peptide were fractionated, and the cytoplasmic (C) and periplasmic (P) fractions were analyzed by Western blotting. Unprocessed full-length constructs (dots) and constructs after signal peptide cleavage (asterisks) are marked. The cytosolic chaperonin GroEL and periplasmic MBP were analyzed in parallel. Translocation efficiency is indicated. Data represent mean Ϯ S.E. of at least three independent experiments.  , 3 h). White arrowhead, unglycosylated protein fraction; black arrowhead, glycosylated protein fraction. Transformed E. coli cells expressing the protein either with the DsbA or PelB signal peptide were fractionated, and the cytoplasmic (C) and periplasmic (P) fractions were analyzed by Western blotting. Unprocessed full-length constructs (dots) and constructs after signal peptide cleavage (asterisks) are marked. The cytosolic chaperonin GroEL and periplasmic MBP were analyzed in parallel. Translocation efficiency is indicated. Data represent mean Ϯ S.E. of at least three independent experiments. B, C-terminally located ␣-helical domains restore secretion of intrinsically disordered domains in mammalian cells. The three proteins depicted were expressed in transiently transfected HeLa cells, and expression was analyzed as described in A. To analyze glycosylation of the proteins, one set of lysates was treated with EndoH (ϩ) before Western blotting. Right, the percentage of the ER-translocated fraction was determined as the ratio between the signal of the glycosylated fractions and the total protein signal. Data represent mean Ϯ S.E. (error bars) of three independent experiments. **, p Ͻ 0.005; ns, not significant. C, intrinsically disordered domains interfere with import into E. coli inner membrane vesicles in vitro. The indicated substrates containing a DsbA signal peptide were translated and labeled with L-[ 35 S]methionine in vitro in the presence (ϩ) or absence (Ϫ) of E. coli INVs. After translation, samples were treated with proteinase K (ϩPK) or left untreated (ϪPK) and analyzed by SDS-PAGE and phosphorimaging. Indicated are the precursor forms of the substrates (dots) and the mature form after cleavage of the signal peptide (asterisks). For the calculation of percentage of integration, the radioactive signals in the ϪPK and ϩPK lanes were quantified using the ImageQuant software. The obtained value for ϪPK was set to 100%, and the value ϩPK was calculated. This latter value was then corrected for the amount of material that was loaded (i.e. 80% of the total in vitro synthesized material). The mean of at least three independent experiments and the S.D. values are indicated.

Impaired Sec61/Y-mediated translocation
manner (22)(23)(24)(25)(26)(27)(28). Our study was designed to address the question of whether secondary structures within the nascent chain serve as additional regulatory elements. The experimental approach was based on the comparative analysis of a set of substrates of similar length but different secondary structure. Importantly, all proteins were equipped with well-studied signal peptides derived from authentic secretory proteins of the respective organisms. Indeed, our results revealed that secondary structure can significantly modulate translocation effi-ciency. Specifically, neither the Sec61 complex in mammalian and yeast cells nor the SecY complex in E. coli can efficiently transport client proteins that are completely intrinsically disordered or exclusively composed of ␤-strands. Increasing the length of these substrates or the use of more efficient signal peptides did not significantly improve ER import or secretion.
The unstructured substrates that failed to be imported contained an uncleaved signal peptide and were subjected to proteasomal degradation in mammalian cells. Thus, we disfavor an Figure 6. Impaired translocation of ␤-strands through the Sec61 and SecY translocon. A and B, top panels, schematic presentation of the constructs analyzed. Dark rectangle, signal peptide; helices, ␣-helical structure derived from PrP; straight line, intrinsic disorder derived from PrP; arrows, ␤-strands derived from GFP; polygons, N-linked glycosylation acceptor site; gray rectangle, GPI anchor signal sequence. The total length of the proteins is indicated. A, impaired activity of ␤-strands to promote ER import of intrinsically disordered domains. HeLa cells were transiently transfected with the constructs indicated and analyzed by Western blotting. To analyze N-linked glycosylation, lysates were treated with PNGase F (ϩ) before Western blotting. White arrowhead, unglycosylated protein fraction; black arrowhead, glycosylated protein fraction. Right, the percentage of the ER-translocated fraction was determined as the ratio between the signal of the glycosylated fractions and the total protein signal. Data represent mean Ϯ S.E. (error bars) of three independent experiments. **, p Ͻ 0.005; ns, not significant. B, proteins composed of ␤-strand are inefficiently transported through the Sec61 and the SecY translocon. The indicated proteins were transiently expressed and analyzed in HeLa cells as described in A. Right, translocation efficiency of constructs was measured densitometrically as described in A. ***, p Ͻ 0.0005; NS, not significant. Bottom panels, transformed E. coli cells expressing the proteins with either the DsbA or PelB signal peptide were fractionated, and the cytoplasmic (C) and periplasmic (P) fractions were analyzed by Western blotting. Unprocessed full-length constructs (dots) and constructs after signal peptide cleavage (asterisks) are marked. The cytosolic chaperonin GroEL and the periplasmic MBP were analyzed in parallel. Translocation efficiency is indicated. Data represent mean Ϯ S.E. of at least three independent experiments.

Impaired Sec61/Y-mediated translocation
interpretation that the proteins were first imported into the ER lumen and then subjected to the ERAD pathway. However, it seems unlikely that the targeting of unstructured proteins to the translocon was impaired. First, in vitro studies employing stalled ribosome nascent chain complexes indicated that also unstructured nascent chains are productively targeted to the Sec61 translocon (34). Second, we used model substrates longer than 200 aa to exclude the possibility that reduced SRP binding to shorter substrates might account for the observed translocation defects. Based on these findings, it seems plausible that unstructured substrates are targeted to the translocon, where the signal peptide initiates early translocation steps. However, translocation is aborted before the signal peptide is cleaved. As a consequence, the unstructured substrate is released from the translocon and subjected to proteasomal degradation. Alternatively, translocon-associated and/or cytosolic components might recognize unstructured domains and ␤-strands and actively interfere with their translocation. For such a model, however, one has to consider that such components have to be present in bacteria, yeast, and mammalian cells and, at least in eukaryotic cells, spare proteins with an extended (Ն180-aa) unstructured N-terminal domain followed by C-terminal ␣-helical domains (see below). In E. coli, the signal peptides of a fraction of the unstructured proteins are processed before translocation is aborted. This possibility is based on the in vivo experiments in which two protein species are often detected in the cytoplasmic fractions and on the observed cleavage of the signal peptide of all constructs in the in vitro import assays after the addition of inverted inner membrane vesicles.
Another interesting finding of our study was the apparent advantage of the SecA-dependent pathway in E. coli for the secretion of ␤-strand proteins. In support of this concept, naturally occurring outer membrane proteins, such as OmpC, OmpD, OmpF, OmpE, and OmpN, which are nearly entirely composed of ␤-strands, are targeted to the SecY complex via SecA.

Efficient translocation requires ␣-helical domains in addition to the signal peptide
Our study emphasized the importance of ␣-helical domains in the mature part of the protein to achieve efficient translocation through both the Sec61 and the SecY translocon. This activity was not only seen for model proteins designed for this study but also for naturally occurring secretory proteins with extended unstructured domains in mammalian cells, such as the GPI-anchored proteins PrP C and Shadoo (this study) and the neuropeptide hormones somatostatin and gonadotropinreleasing hormone (34,35). As mentioned above, targeting of the ribosome-nascent chain complex of unstructured proteins to the translocon was apparently not impaired (34), indicating a role of ␣-helical domains in a post-targeting event. Which step may be affected? After targeting of the ribosome-nascent chain complex to the translocon, the signal peptide also contributes to the opening of the translocation channel (67)(68)(69)(70). Thus, it is conceivable that after the initial gating by the signal peptide, additional ␣-helical domains are required to keep the pore open and to ensure efficient translocation into the ER lumen. Once the N-terminal domain of a protein is in the ER lumen, the secondary structure of the remaining part is of minor importance because folding of the mature part and interaction with lumenal proteins, such as BiP, provide a driving force for complete translocation. This concept would also explain why ␣-helical domains are most potent when they are located next to the signal peptide, because then the pore is already in an open conformation. Located further downstream, gating may already have ceased, making it more difficult for the C-terminally located ␣-helical domain to re-initiate gating.
Notably, the activity of ␣-helical domains to promote secretion may also be of physiological relevance to SecY-mediated translocation in E. coli. By inspecting the secondary structure of naturally occurring ␤-barrel proteins, such as OmpF, OmpC, and OmpE, we noticed that in addition to ␤-strands, short ␣-helical domains are present at conserved positions.

Adaptive pathways in eukaryotic cells enable Sec61-mediated translocation of secretory proteins with extended N-terminal unstructured domains
Another important finding of our study was that abortive ER import of unstructured domains can be restored by ␣-helical domains, arguing against a dominant negative activity of these domains during translocation. Moreover, our comparative analysis in bacteria and eukaryotic cells revealed organismspecific differences. Located N-terminally to an unstructured domain, ␣-helical domains promoted translocation through both the Sec61 complex in mammalian cells and the SecY complex in bacteria. Interestingly, however, the activity of N-terminally located ␣-helical domains to rescue IDD translocation was rather poor in E. coli compared with the activity in mammalian cells. Located at the C terminus of the unstructured protein, ␣-helical domains were still proficient in promoting translocation in eukaryotic cells, but not in bacteria. An attractive model to explain the activity of C-terminal ␣-helical domains in mammalian cells is based on the findings that nascent polypeptides can pause on the cytosolic side of the translocon before productive translocation (43,45,71). Indeed, it was shown before that translocation of the unstructured N-terminal domain of the prion protein was delayed until the ␣-helical domains in the C terminus were synthesized (45). Moreover, delayed translocation induced recruitment of translocon accessory factors, such as Sec62 and Sec63, components that are missing in E. coli. Thus, one could envisage a pathway in mammalian cells allowing secretory proteins with extended unstructured domains to remain at the cytosolic side of the translocon until ␣-helical domains are synthesized to promote translocation. Bacteria seem to lack such a pathway to handle secretory proteins with extended N-terminal unstructured domains. Interestingly, structural disorder within the proteome increased with the transition from prokaryotic to eukaryotic cells (72), consistent with the notion that intrinsically disordered proteins play a major role in several eukaryotic protein classes including signaling molecules (reviewed in Refs. [73][74][75][76][77][78]. Hence, the apparent translocation deficiency is probably of little relevance to bacteria, because secretory proteins with extended N-terminal unstructured domains have not been described so far in prokaryotes. In eukaryotic cells, adaptive mechanisms may have evolved in parallel with the expansion of intrinsically Impaired Sec61/Y-mediated translocation disordered domains in the proteome to compensate for the inherent translocation deficiency. It will now be interesting to identify these putative translocon-associated pathways in eukaryotic cells and study their role in the regulation of translocation efficiency in a context-and substrate-dependent manner.

Studies in mammalian cells
Human HeLa cells were cultured in DMEM (Thermo Fisher Scientific) with the addition of 10% fetal calf serum, 100 units/ml penicillin, and 100 g/ml streptomycin. They were grown in a humidified 5% CO 2 atmosphere at 37°C. Cells cultivated on a 3.5-cm cell culture dish (Nunc, Roskilde, Denmark) were transfected with a total of 1 g of DNA by a liposomemediated method, using Lipofectamine Plus reagent (Life Technologies) according to the manufacturer's instructions. For proteasomal inhibition cells were treated with 30 M MG-132 (Sigma-Aldrich) for 3-5 h at 37°C before cell lysis. To deglycosylate proteins, cell lysates were treated with PNGase F (New England Biolabs) or endoglycosidase H (New England Biolabs) for 1 h at 37°C according to the manufacturer's instructions. For Western blot analysis, cells were washed twice with cold saline buffer (PBS), scraped off the plate, pelleted by centrifugation (5,000 ϫ g, 5 min), and lysed in detergent buffer (0.5% Triton X-100, 0.5% sodium deoxycholate in PBS). The cell lysates were either analyzed directly or centrifuged (20,000 ϫ g, 10 min) to analyze the postnuclear supernatant.

Studies in S. cerevisiae
For expression in S. cerevisiae, constructs bearing the Kre5p signal peptide were cloned into pYES2 expression vector and transformed into K39 wild-type yeast cells according to established protocols (80). A single clone of transformed K39 wildtype yeast cells was picked and inoculated in 20 ml of ϪUraϩGlu medium. Cells were grown overnight at 30°C and 120 rpm in the presence of glucose. The next day, cells were centrifuged (2,000 ϫ g, 5 min), and the pellet was resuspended in ϪUraϩGal medium (0.4% (w/v) galactose, 0.1% (w/v) yeast extract, 0.5% (w/v) ammonium sulfate, 0.17% (w/v) YNB-amino acid mix (pH 6.0)) to induce gene expression. Cells were harvested (2,000 ϫ g, 5 min) and lysed using glass beads (Alekto

Studies in E. coli
Constructs bearing DsbA or PelB signal peptide were cloned into pET27b(ϩ) (Novagen) and transformed in E. coli BL21 (DE3) (Thermo Fisher Scientific). Main expression cultures were inoculated from overnight culture to an optical density of A 600 nm ϭ 0.05 and grown in LB substituted with 50 mg/ml kanamycin at 30°C at 120 rpm. Protein expression was induced at A 600 nm ϭ 0.5 for 2 h by the addition of 1 mM isopropyl 1-thio-␤-D-galactopyranoside. The isolation of the periplasmic fraction was described previously (81). In brief, 10 ml of main culture were harvested by centrifugation at 4,000 ϫ g for 1 min at 4°C. The cell pellet was carefully resuspended in TSE periplasmic extraction buffer (200 mM Tris-HCl, pH 8.0, 500 mM sucrose, 1 mM EDTA, cOmplete Mini EDTA-free protease inhibitor mixture (Roche Applied Science)) using a wire loop. Respective volume of TSE for resuspension was calculated according to A 600 nm of the main culture at the time of harvesting (A 600 nm ϭ 1.0 Ϸ 1.000 l of TSE buffer). After a 30-min incubation on ice, cells were centrifuged for 30 min at 21,000 ϫ g and 4°C. Supernatant containing periplasmic fraction was isolated for Western blotting. The cell pellet was resuspended in TE buffer (same volume as TSE buffer, 10 mM Tris-HCl, 1 mM EDTA, pH 8, cOmplete Mini EDTA-free protease inhibitor mixture (Roche Applied Science)), and cells were opened by sonification and again centrifuged for 30 min at 21,000 ϫ g and 4°C to obtain soluble cytoplasmic fraction for Western blotting.

Western blotting
For Western blot analysis, lysates were boiled in Laemmli sample buffer with ␤-mercaptoethanol (4% (v/v)). Following SDS-PAGE, proteins were transferred to nitrocellulose by electroblotting. Membranes were blocked by incubation in TBS-T (TBS with 0.1% (w/v) Tween 20) containing 5% skimmed milk for 1 h at room temperature and incubated with primary antibody in TBS-T ϩ 5% skimmed milk overnight at 4°C. After washing with TBS-T, blots were incubated with respective secondary antibody (IRDye-Infrared Technology, LI-COR) in TBS-T for 1 h at room temperature. Protein signals were visualized and quantified using an ODYSSEY 9120 scanner and Image Studio Light software (LI-COR).

Quantification of translocation efficiency
To quantify Sec61-mediated translocation, the respective bands of the constructs were measured densitometrically, and the percentage of glycosylated protein relative to total protein amount was determined. For E. coli/SecY, translocation efficiency was calculated as the ratio of substrate present in the periplasmic fraction normalized to the relative amount of the periplasmic protein MBP in the periplasmic fraction. Specifically, the translocation efficiency of MBP was set as 100% and assumed that the fraction of MBP present in the cytoplasmic fraction is due to an incomplete lysis of bacteria by cold osmotic shock. For example, when 30% of MBP was detected in the cytosolic fraction, the amount of our substrate present in the cytoplasmic fraction was reduced by 30% for the calculation of the ratio between cytoplasmic and periplasmic localization. For quantification, both the species representing the full-length unprocessed protein (marked by a dot) and the protein with the signal peptide cleaved off (marked by an asterisk) were included. Note that only the soluble fractions of the proteins were analyzed. Data represent mean Ϯ S.E. of at least three independent experiments for each construct. The significance was determined by using Student's t test (**, p Ͻ 0.005; ***, p Ͻ 0.0005).

In vitro protein synthesis and transport
The components of the in vitro system, salt-washed ribosomes, initiation factors, and the cytosolic translation factors were prepared as described previously (65,82). In vitro protein synthesis was performed in 50-l aliquots in the presence of 1 l of E. coli inverted inner membrane vesicles (ϳ50 g of protein). The reaction was started by the addition of 5 units of T7 RNA polymerase and 8 units of placental RNase inhibitor. Incubation time was 30 min at 37°C. Samples were subsequently divided into two aliquots of 10 and 40 l each. The 10-l aliquot was directly precipitated with ice-cold TCA (final concentration 10%), whereas the 40-l aliquot was incubated with 40 l of proteinase K (PK) (0.3 mg/ml, incubation on ice for 15 min). TCA was subsequently added (10% final concentration), and the sample was incubated at 56°C for 10 min, followed by a 30-min incubation at 4°C. The samples were then centrifuged for 20 min at 13,000 rpm, and the pellet was resuspended in 20 l of SDS protein loading dye. Samples were separated by 15% SDS-PAGE and analyzed by phosphorimaging and Image Quant software (Storm PhosphorImager, GE Healthcare). For the calculation of percentage of integration, the radioactive signals in the ϪPK and ϩPK lanes were quantified using the ImageQuant software. The obtained value for ϪPK was set to 100%, and the value ϩPK was calculated. This latter value was then corrected for the amount of material that was loaded (i.e. 80% of the total in vitro synthesized material).
Author contributions-A. G., S. J., S. U., A. O., A. Z. acquired data; M. B. contributed essential reagents; H.-G. K. and R. Z. analyzed and interpreted data; K. F. W. and J. T. conceived and designed the study, analyzed and interpreted data, and drafted the manuscript. All authors reviewed the results and approved the final version of the manuscript.