Tyrosine hydroxylase down-regulation after loss of Abelson helper integration site 1 (AHI1) promotes depression via the circadian clock pathway in mice

Abelson helper integration site 1 (AHI1) is associated with several neuropsychiatric and brain developmental disorders, such as schizophrenia, depression, autism, and Joubert syndrome. Ahi1 deficiency in mice leads to behaviors typical of depression. However, the mechanisms by which AHI1 regulates behavior remain to be elucidated. Here, we found that down-regulation of expression of the rate-limiting enzyme in dopamine biosynthesis, tyrosine hydroxylase (TH), in the midbrains of Ahi1-knockout (KO) mice is responsible for Ahi1-deficiency–mediated depressive symptoms. We also found that Rev-Erbα, a TH transcriptional repressor and circadian regulator, is up-regulated in the Ahi1-KO mouse midbrains and Ahi1-knockdown Neuro-2a cells. Moreover, brain and muscle Arnt-like protein 1 (BMAL1), the Rev-Erbα transcriptional regulator, is also increased in the Ahi1-KO mouse midbrains and Ahi1-knockdown cells. Our results further revealed that AHI1 decreases BMAL1/Rev-Erbα expression by interacting with and repressing retinoic acid receptor–related orphan receptor α, a nuclear receptor and transcriptional regulator of circadian genes. Of note, Bmal1 deficiency reversed the reduction in TH expression induced by Ahi1 deficiency. Moreover, microinfusion of the Rev-Erbα inhibitor SR8278 into the ventral midbrain of Ahi1-KO mice significantly increased TH expression in the ventral tegmental area and improved their depressive symptoms. These findings provide a mechanistic explanation for a link between AHI1-related behaviors and the circadian clock pathway, indicating an involvement of circadian regulatory proteins in AHI1-regulated mood and behavior.

Here, we demonstrate that AHI1 affects mood and behavior through circadian protein-mediated TH expression. AHI1 binds to ROR␣ and represses BMAL1 expression, subsequently inhibits Rev-Erb␣ expression, and in turn up-regulates TH expression. Loss of AHI1 increases BMAL1 and Rev-Erb␣ levels and leads to TH expression inhibition.

Decreased TH expression in Ahi1 KO mouse midbrains and Ahi1 knockdown cells
The previous studies demonstrated that Ahi1 KO mice present depression-like behaviors with a significant decrease of DA and 5-HT levels in many brain regions (10,11). Considering that there are no differences in behaviors and AHI1 expression between Ahi1 heterozygous (Ahi1 ϩ/Ϫ ) and wildtype mice (10,11), we used Ahi1 ϩ/Ϫ mice as controls and investigated the depressive behaviors of Ahi1 KO mice. Ahi1 KO mice showed a significant increase of immobility as compared with controls in tail suspension test (TST) and forced swim test (FST) (Fig. 1, A and B), indicating that Ahi1 deficiency indeed leads to depression in mice. To identify how AHI1 affects production or release of DA and 5-HT, we measured mRNA levels of the key enzymes and transporters that are involved in production or release of DA and 5-HT in Ahi1 KO mouse midbrains and the littermate controls, including 5-HT biosynthetic enzymes tryptophan hydroxylase 1 and 2 (TPH1 and TPH2), DA biosynthetic enzyme TH and DOPA decarboxylase (DDC), 5-HT and DA metabolic enzymes MAOA and MAOB, DA metabolic enzyme catechol-O-methyltransferase (COMT), 5-HT transporter (5-HTT), DA transporter (DAT), and vesicular monoamine transporter 2 (VMAT2). Interestingly, TH mRNA levels but not others were significantly decreased in Ahi1 KO mouse midbrains (Fig. 1C). Consistently, TH protein levels but not other detected proteins were decreased in Ahi1 KO mouse midbrains (Fig. 1D). Interestingly, stereological analysis revealed that there is no difference of TH-positive neuron numbers in the ventral tegmental area (VTA) between Ahi1 KO mice and the littermate controls using diaminobenzidine (DAB) staining ( Fig. 1, E and F). However, immunofluorescence staining showed that the TH fluorescence intensity was significantly decreased in VTA of Ahi1 KO mice compared with the litter-mate controls (Fig. 1, G and H). These data suggest that Ahi1 KO mice have lower TH protein expression levels but no changes in the cell numbers of TH neurons.
Next, we verified the regulation of TH by AHI1 in cultured cells. In Neuro-2a (N2a) cells, knockdown of Ahi1 by two targeted siRNAs significantly decreased both TH mRNA and TH protein levels (Fig. 1, I and J). Meanwhile, overexpression of exogenous EGFP-AHI1 markedly increased TH protein levels ( Fig. S1A). To further identify whether AHI1 regulates TH promoter activity, we cloned a 2-kb promoter fragment of murine TH gene and inserted it into a luciferase reporter vector. Overexpression of EGFP-AHI1 in cells dramatically increased TH promoter-driven luciferase reporter activity (Fig. S1B).
Many studies indicated that expression of TH is a time-ofday circadian oscillating pattern, and its expression is lowered at daytime and raised at night in mouse brain (23,28,30). Therefore, we tested whether AHI1 affects TH expression at different times of the day. As shown in Fig. 1K, TH protein levels are higher at subjective dawn (circadian time 00, CT00) than at subjective dusk (CT12) in both Ahi1 ϩ/Ϫ and Ahi1 Ϫ/Ϫ mouse midbrains. Moreover, TH protein levels are significantly lower in Ahi1 KO mice midbrain than littermate controls at both CT00 and CT12 (Fig. 1, K and L). Taken together, our results suggested that Ahi1 KO mice exhibit depression-like behaviors, at least in part, by decreasing TH transcriptional expression.

Increased Rev-Erb␣ expression in Ahi1 KO mouse midbrains and Ahi1 knockdown cells
NURR1 is a major transcriptional factor of TH, and the circadian nuclear receptor Rev-Erb␣ represses TH transcription via competing with NURR1 to bind to RRE/NBRE elements of the TH promoter (23,25). Therefore, we measured whether AHI1 has an effect on expression of NURR1 or Rev-Erb␣. In Ahi1 KO mouse midbrains, Rev-Erb␣ protein levels were dramatically increased compared with the littermate controls; however, NURR1 protein levels kept unchanged (Fig. 2, A and  B). Real-time qPCR analysis showed that Rev-Erb␣ but not Nurr1 mRNA levels were elevated in Ahi1 KO mouse midbrains (Fig. 2C). In cultured N2a cells, knockdown of Ahi1 using siRNAs markedly increased Rev-Erb␣ protein as well as Rev-Erb␣ mRNA levels but did not affect NURR1 protein and mRNA levels (Fig. 2, D and E). We next identified the correlation between the decrease of TH and increase of Rev-Erb␣ expression caused by AHI1 deficiency. Knockdown of Rev-er␣ increases TH protein levels in cells (Fig. S2), consistent with a study by other investigators (23). We next cloned a mutant TH promoter that lacks a Rev-Erb␣-binding site and investigated the effect of AHI1 on its activity (Fig. 2F). Interestingly, EGFP-AHI1 lost the ability to influence the mutant TH promoter activity (Fig. 2G). These results suggest that AHI1-mediated TH expression is dependent on negatively regulating Rev-Erb␣ but not NURR1 expression. In contrast to the TH circadian pattern (Fig. 1, K and L), Rev-Erb␣ protein levels are lower at CT00 than at CT12, and it is increased in Ahi1 KO mouse midbrain compared with littermate controls at both CT00 and CT12 (Fig. 2, H and I).

The role of AHI1 in depressive behaviors
Induction of Rev-Erb␣ expression by Ahi1 deficiency is BMAL1-dependent BMAL1/CLOCK binds to the E-box element of the Rev-Erb␣ promoter to activate its transcription (31,32). We therefore constructed two luciferase reporter vectors, a fragment of promoter in the Rev-Erb␣ gene (Ϫ1482/ϩ502) (WT-Luc) and its deletion mutant (⌬CACATG (ϩ24/ϩ29)) (mutant-Luc), which lacks the BMAL1/CLOCK-binding site (Fig. 3A), to identify whether BMAL1 is involved in the regulation of Rev-Erb␣ by AHI1. Overexpression of EGFP-AHI1 repressed both basal and BMAL1/CLOCK-induced activities of the Rev-Erb␣ promoter (Fig. 3A). However, AHI1 did not affect activity of the mutant Rev-Erb␣ promoter (Fig. 3A). As the BMAL1/CLOCK-binding site in the Rev-Erb␣ promoter is involved in AHI1-regulated Rev-Erb␣ transcription, we examined whether BMAL1 and CLOCK are regulated by AHI1. In Ahi1 knockdown cells, BMAL1 but not CLOCK protein levels were increased (Fig. 3B). Similar to results from in vitro assays, BMAL1 but not CLOCK protein levels were increased in Ahi1 KO mouse midbrains (Fig.  3C). Moreover, Bmal1 but not Clock mRNA levels were also increased in Ahi1 knockdown cells (Fig. 3D), suggesting that AHI1 may transcriptionally regulate BMAL1 expression. Other clock genes, such as Periods (Per1, Per2, and Per3) and Crytochromes (Cry1 and Cry2), are also transcriptionally controlled by BMAL1 (33). We therefore examined Pers and Crys mRNA levels and found that Pers and Crys genes were also up-regulated in Ahi1 KO mouse midbrains (Fig. S4). BMAL1 transcription is activated by transcriptional factor ROR␣ (34). We next performed immunoprecipitation assays to examine the interactions between AHI1 and ROR␣. FLAG-ROR␣ was co-precipitated when EGFP-AHI1 but not EGFP was precipitated using anti-GFP antibodies (Fig. 3E). We also performed immunocytochemistry staining to detect the distribution of AHI1 and ROR␣. Although EGFP-AHI1 was mainly distributed in cytoplasm, it partly localized in the nucleus and co-localized with FALG-ROR␣ (Fig. 3F). Next, we examined whether AHI1 influences Bmal1 promoter activity through ROR␣. The schematic diagrams of Bmal1 promoter luciferase reporter and its deletion mutant lacking two ROREs that are responsible for ROR␣ binding are shown (Fig. 3G). EGFP-AHI1 significantly repressed basal activity and ROR␣-induced activity of the Bmal1 promoter (Fig. 3G). However, EGFP-AHI1 did not repress activity of the mutant Bmal1 promoter (Fig. 3G). These data suggest that AHI1 interacts with ROR␣ to repress Bmal1 transcription.

Regulation of TH by AHI1 is BMAL1-dependent
We have shown that AHI1 deficiency reduces TH expression and up-regulates Rev-Erb␣ levels and that overexpression of AHI1 decreases BMAL1 expression. We wondered whether the regulation of TH expression by AHI1 is mediated by BMAL1regulated Rev-Erb␣ expression. In Bmal1 knockdown cells, increase of TH and decrease of Rev-Erb␣ protein levels were observed (Fig. 4A). Moreover, TH and Rev-Erb␣ mRNA levels were altered simultaneously in Bmal1 knockdown cells (Fig.  4B). We next examined the effects of BMAL1/CLOCK on TH promoter activity. BMAL1/CLOCK dramatically repressed TH promoter activity, and BMAL1/CLOCK had no significant effect on the mutant TH promoter lacking the Rev-Erb␣binding site, indicated by reporter gene assays (Fig. S3). Moreover, increase of Rev-Erb␣ and decrease of TH protein levels induced by Ahi1 knockdown were completely eliminated by BMAL1 deficiency (Fig. 4C), further suggesting that AHI1-induced TH expression is mediated by the BMAL1/CLOCK/Rev-Erb␣ circadian pathway.

Rev-Erb␣ inhibition prevents TH reduction and improves depressive behaviors of Ahi1 KO mice
As Rev-Erb␣ up-regulation contributes to the decreased TH expression induced by AHI1 deficiency, we wondered whether Rev-Erb␣ inhibition has impacts on TH expression and behavior of Ahi1 KO mice. We microinfused SR8278, a Rev-Erb␣ inhibitor, into the VMB of Ahi1 KO mice or control mice to inhibit midbrain Rev-Erb␣ activity. We examined TH expressions in VTA after SR8278 microinfusion. Although SR8278 did not significantly affect TH levels in control mice, TH levels were dramatically increased in VTA in Ahi1 KO mice after SR8278 administration (Fig. 5, A and B). In addition, SR8278 administration significantly improved the performance of Ahil KO mice in TST but did not significantly affect the control mice ( Fig. 5C).

Discussion
In the present study, we revealed that AHI1 regulates TH transcriptional expression through the circadian ROR␣/ BMAL1/Rev-Erb␣ pathway, to participate in mood and behavior regulation. TH is a rate-limiting enzyme of biosynthesis of catecholamines, such as DA, noradrenaline, and epinephrine, by converting tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA) (35). It has been reported that the TH gene is associated with depressive disorder or bipolar disorder (36,37). Our study demonstrates that AHI1-regulated TH expression may con- Figure 1. Decreased TH expression in Ahi1 KO mouse midbrains and Ahi1 knockdown cells. A, immobility time in TST was measured in control mice (n ϭ 10) and Ahi1 KO mice (n ϭ 7). **, p Ͻ 0.01. B, immobility time in FST was measured in control mice (n ϭ 10) and Ahi1 KO mice (n ϭ 6). **, p Ͻ 0.01. C, relative mRNA levels of the indicated genes of Ahi1 KO mouse midbrains and littermate controls were performed by real-time qPCR. **, p Ͻ 0.01 (n ϭ 5). D, the indicated protein abundance of Ahi1 KO mouse midbrains and the littermate controls was performed by Western blot analysis. The levels of the indicated proteins relative to GAPDH are shown on the right. **, p Ͻ 0.01 (n ϭ 3). E, representative images of TH-DAB staining in VTA of Ahi1 KO mice and the littermate controls are shown at AP Ϫ3.5 mm. F, quantification of the TH-positive cells from (E) was shown. ns, no statistical significance (n ϭ 3). G, representative images of TH-fluorescence staining in VTA of Ahi1 KO mice and the littermate controls are shown at AP Ϫ3.5 mm. H, intensity of TH immunofluorescence signals in G was analyzed. ***, p Ͻ 0.001 (n ϭ 3). I, N2a cells were transfected with the indicated siRNAs. Seventy-two h after transfection, real-time qPCR was performed. *, p Ͻ 0.001 (n ϭ 3). J, N2a cells were transfected with indicated siRNAs. Seventy-two h after transfection, the total cell lysates were subjected to immunoblot analysis. The intensities of AHI1 or TH relative to GAPDH (right) were analyzed. ***, p Ͻ 0.001 (n ϭ 3). K, TH protein abundance of Ahi1 KO mouse midbrain and littermate controls at CT12 and CT00 by Western blot analysis. L, the relative ratios of TH to GAPDH in I were analyzed from density analysis by one-way ANOVA. Data are presented as means Ϯ S.E. (error bars). **, p Ͻ 0.01 (n ϭ 3).  3). B, the relative levels of the indicated proteins to GAPDH in A were analyzed. ***, p Ͻ 0.001; ns, no statistical significance (n ϭ 3). C, relative mRNA levels of Rev-Erb␣ and TH of Ahi1 KO mouse midbrains and littermate controls were examined using real-time qPCR. ***, p Ͻ 0.001; ns, no statistical significance (n ϭ 5). D, N2a cells were transfected with the indicated siRNAs. Seventy-two h after transfection, the cell lysates were subjected to immunoblot analysis. The relative levels of Rev-Erb␣ and NURR1 to GAPDH (bottom) were analyzed. ***, p Ͻ 0.001; ns, no statistical significance (n ϭ 3). E, N2a cells were transfected with the indicated siRNAs. Seventy-two h after transfection, real-time qPCR was performed. ***, p Ͻ 0.001; ns, no statistical significance (n ϭ 3). F, schematic representation of mutant TH (mTH) promoter that lacks the Rev-Erb␣ binding motif in PGL3-Basic vector. G, HEK293 cells were transfected with PGL3-TH-Luc or PGL3-mTH-Luc along with EGFP or EGFP-AHI1, respectively. Forty-eight h after transfection, luciferase reporter assays were performed. ***, p Ͻ 0.001; ns, no statistical significance (n ϭ 3). H, Rev-Erb␣ protein abundance of Ahi1 KO mouse midbrain and littermate controls at CT12 and CT00 by Western blot analysis. I, the relative ratios of Rev-Erb␣ to GAPDH in H were analyzed from density analysis by one-way ANOVA. Data are presented as means Ϯ S.E. (error bars). ***, p Ͻ 0.001; **, p Ͻ 0.01 (n ϭ 3).

The role of AHI1 in depressive behaviors
tribute to mood and behavior regulation. Decreased TH levels in the Ahi1 KO mouse midbrain explain the decreased DA levels responsible for animal depressive behaviors.
AHI1 has comprehensive roles in mental regulation. Ahi1 KO mice also show depressive phenotypes (10,11). AHI1 is abundant in the hypothalamus and amygdale, the regions that are important for emotional regulation, and loss of AHI1 influences TrkB signaling involved in depression behaviors (11,38). In our study, we further found that AHI1 deficiency induces decreases of TH levels in VTA. As DA in VTA is important for emotional regulation, our study suggests an involvement of DA in AHI1-deficiency-induced depressive behavior. TH expression is activated by NURR1 or repressed by circadian clock protein Rev-Erb␣ through competing for binding to the NBRE motif in the TH promoter (23,25). In our observation, AHI1 does not affect NURR1 expression, but AHI1 deficiency induces Rev-Erb␣ expression. In addition, TH promoter activity that lacks the NBRE motif cannot be regulated by AHI1, further suggesting that Rev-Erb␣ is involved in AHI1-regulated TH expression. It was reported that loss of AHI1 impairs TrkB  3). B, N2a cells were transfected with the indicated siRNAs. Seventy-two h after transfection, the cell lysates were subjected to immunoblot analysis. The intensities of BMAL1 and CLOCK relative to GAPDH (bottom) were analyzed. **, p Ͻ 0.01 (n ϭ 3). C, the BMAL1 and CLOCK protein levels of Ahi1 KO mouse midbrains and the littermate controls were examined using immunoblot analysis (n ϭ 3). The intensity of BMAL1 relative to GAPDH (bottom) was analyzed. **, p Ͻ 0.01 (n ϭ 3). D, N2a cells were transfected with the indicated siRNAs. Seventy-two h after transfection, real-time qPCR was performed. ***, p Ͻ 0.001; ns, no statistical significance (n ϭ 3). E, HEK293 cells were transfected with FLAG-ROR␣ along with EGFP or EGFP-AHI1. Forty-eight h after transfection, an immunoprecipitation assay was performed with anti-GFP antibodies. F, HEK293 cells were transfected with FLAG-ROR␣ and EGFP-AHI1 (green). Forty-eight h after transfection, immunofluorescence was performed with anti-FLAG (red). The nuclei were stained with DAPI (blue). G, left, a schematic representation shows the WT and mutant Bmal1 promoter luciferase reporter. Right, HEK293 cells were transfected with WT-Luc or mutant-Luc of Bmal1 promoter along with EGFP, EGFP-AHI1 or FLAG, FLAG-ROR␣, respectively. Forty-eight h after transfection, a luciferase reporter assay was performed. ***, p Ͻ 0.001; ns, no statistical significance (n ϭ 3). Error bars, S.E. IP, immunoprecipitation; IB, immunoblotting.

The role of AHI1 in depressive behaviors
signaling (11). Interestingly, TrkB signaling activates TH expression through activating its promoter (39). In addition, TrkB activity is also regulated by the circadian clock pathway, showing that TrkB activity is higher at night and lower during the day (40). Whether AHI1-mediated circadian rhythm regulation influences TrkB signaling and whether TrkB participates in AHI1-mediated TH regulation remain to be evaluated.
The core circadian proteins CLOCK and BMAL1 form heterodimers to activate the transcription of several clock genes, such as PERs, CRYs, ROR␣, and Rev-Erb␣. In turn, PERs and CRYs form complexes to repress the transcriptional activity of CLOCK/BMAL1. On the other hand, ROR␣ and Rev-Erb␣ competitively bind to ROREs in the BMAL1 promoter to activate or repress its transcription, respectively (33).
The association between circadian rhythms and mood regulation as well as behavior processes has been well-documented (41). Disruption of biological rhythms is considered as a hallmark for and one of the key contributors to several mental disorders, such as major depression (42,43), bipolar disorder (44), and SCZ (45). Polymorphisms in several circadian genes have been identified to associate with mental disorders (20,45,46). Recently, many studies showed that circadian gene deficiency is associated with mood and behavior abnormalities in animals (47). For example, mice harboring the Clock mutant gene exhibit mania-like behavior with increased dopaminergic activity (26,28), and Per2 and Bmal1 KO mice also display mania-like response (29,48,49). Interestingly, inhibition of Rev-Erb␣ by gene KO or inhibitor leads to emotional instability with mania-like behavior and hyperactivity by increasing TH protein levels and dopamine production (23). We also found that microinfusion of Rev-Erb␣ inhibitor SR8278 into the VMB of Ahi1 KO mice significantly increases TH expression and reduces animal immobility time in TST. However, SR8278 only slightly increases TH expression and improves the control mouse performance in TST. The reason may be that Rev-Erb␣ is abundant in Ahi1 KO mice as compared with control mice; thus, Ahi1 KO mice are more sensitive to SR8278. This phenomenon is in accordance with the fact that SR8278 significantly reduces immobility time in Rev-Erb␣ high expression periods in a day rather than that in Rev-Erb␣ low expression periods (23). Thus, deficient Rev-Erb␣ activity in Rev-Erb␣ KO mice induces mania-like behavior, but increased Rev-Erb␣ levels in Ahi1 KO mice result in depressive behavior; both show associations with alterations of TH and DA.
Although several E-box motifs are present in human, mouse, and rat TH promoters (23,35), TH promoter activity cannot be activated by BMAL1/CLOCK in our observations. Conversely, BMAL1/CLOCK dramatically represses TH promoter activity, consistent with a reported study indicating that TH mRNA levels are augmented in Clock ⌬19 mutant mice (26). BMAL1/ CLOCK can bind to the E-box of PER1 and activating transcription factor 5 (ATF5) promoters but not TH promoter by an electrophoretic mobility shift assay (30). These data suggest that BMAL1/CLOCK negatively regulate TH transcription by an indirect pathway. Considering that BMAL1/CLOCK activates Rev-Erb␣ transcription and Rev-Erb␣ directly represses TH expression (23,31) and that Rev-Erb␣ and TH mRNA levels are attenuated and increased in Clock ⌬19 mice (20,26,28), we propose that BMAL1/CLOCK inhibits TH expression via activating Rev-Erb␣ expression.

Figure 5. Restoration of TH levels and improvement of depressive behaviors of Ahi1 KO mice by Rev-Erb␣ inhibition.
A-C, SR8278 (16 g/mouse) or an equal volume of DMSO was microinfused into the VMB of control mice or Ahi1 KO mice. After 2 days, immunohistochemistry and TST were performed. A, fluorescence-TH staining of representative images in VTA were shown at AP Ϫ3.5 mm. B, intensity of TH immunofluorescence signals in A was analyzed. *, p Ͻ 0.05; ns, no statistical significance (n ϭ 3). C, immobility time in TST was measured. Data are presented as means Ϯ S.E. (error bars). **, p Ͻ 0.01; ns, no statistical significance (n ϭ 6 -8).

The role of AHI1 in depressive behaviors
In conclusion, we identified that AHI1 binds to circadian protein ROR␣ to negatively regulate BMAL1 and Rev-Erb␣ expressions, which eliminates Rev-Erb␣-repressive effects and subsequently up-regulates TH expression, suggesting that AHI1 facilitates TH expression and DA biosynthesis through its regulating circadian clock pathway. Loss of AHI1 leads to an increase of Rev-Erb␣ that negatively regulates TH expression (Fig. 6). In conclusion, the present study demonstrates that the mood-related protein AHI1 links the molecular circadian clock pathway to regulate TH expression.

Animals
Ahi1 ϩ/Ϫ and Ahi1 homozygous (Ahi1 Ϫ/Ϫ ) littermate mice were derived from Ahi1 ϩ/Ϫ and Ahi1 Ϫ/Ϫ mice that were described previously (10). All animals were used according to the institutional guidelines for animal use and care, and all procedures were approved by the ethical committee of Soochow University. 8 -10-week-old mice were housed in quarters under a 12-h light/12-h dark photoperiod (light on at 9:00 a.m.) and fed abundant food and water. Mice were sacrificed for collecting brain tissues at 2:00 -4:00 pm except where specially indicated. CT00 was defined as lights on in the previous light/dark schedule when the mice were kept in constant darkness for 2 days.

Depression-like behaviors
The TST and FST were performed at 2:00 -4:00 p.m. as described previously (10,11). For TST, mice were hung in the 40-cm-high shelves by taping the tail (1-2 cm from tip). The immobility time was measured for 6 min. Mice were considered immobile when they gave up escaping or hung passively. For FST, mice were placed into a transparent beaker (16-cm diameter, 23-cm height) containing water (21-25°C) at a depth of 15 cm. After a habituation period of 2 min, immobile time was measured within 4 min. Mice were considered as immobile when they floated or had slight limb movements.

Immunofluorescence and immunohistochemistry
Immunostaining was carried out as described previously (51). Mice were perfused with saline, followed by PBS (pH 7.4) containing 4% paraformaldehyde. Brains were removed and post-fixed in 4% paraformaldehyde overnight at 4°C and then dehydrated with 30% sucrose for 2 days at 4°C. After dehydration, the brain was cut into 30-m-thick coronal sections including the VTA with freezing microtome (CM1900, Leica). Slices were incubated with anti-TH (AB152, Millipore) antibodies for 4 h. For immunofluorescence, slices were stained with rhodamine (red)-conjugated secondary antibody (Invitrogen) for 2 h. Finally, the slices were observed with an inverted system microscope Ti2-E (Nikon, Japan). TH immunofluorescence signals were also quantified by ImageJ software (National Institutes of Health). For immunohistochemistry, slices were incubated with anti-TH antibodies for 4 h, followed by the DAB staining using a non-biotin detection system (GTVision III Anti-Mouse/Rabbit-HRP; Gene Tech), and then slices were observed with an inverted system microscope Ti2-E.

Stereology and image analysis
To determine cell numbers and intensity of TH-immunoreactive neurons in the VTA, an unbiased stereological method

The role of AHI1 in depressive behaviors
was performed according to the optical fractionator principle (52). Briefly, every fifth section (120-m interval) was selected from each mouse and processed for immunostainings for TH using DAB staining. All images were acquired under the same conditions. The numbers of TH-immunoreactive neurons in the VTA were counted after outlining cell bodies and processes using ImageJ software.

Drug application
Mice were anesthetized by intraperitoneal injection of 4% chloral hydrate. Mice were placed prone, and heads were mounted in a stereotaxic apparatus (RWD Life Science Co, Shenzhen, China) by hooking incisors and inserting the ear stick into external auditory canal. Mouse skulls were exposed by cutting the scalp and corroding the meninges with H 2 O 2 . Bregma's location was set to the following: AP 0.0 mm, ML 0.0 mm, DV 0.0 mm. SR8278 was dissolved in DMSO to a concentration of 32 g/l and loaded into a 2.5-l Hamilton syringe, which was fixed on a stereotaxic apparatus. SR8278 was infused into the VMB (AP Ϫ3.5 mm, ML Ϯ1.2 mm, DV Ϫ4.5 mm) (16 g/mouse; a dose was performed according to the previous study (23)) at a rate of 0.1 l/min. After injection, the injector needle was slowly pulled out at a uniform speed in 2 min to avoid bleeding. The mouse scalp was sutured, and mice were placed in a warm environment until they woke up naturally. TST and immunohistochemistry were executed 2 days after microinfusion.

Luciferase reporter assay
HEK293 cells were cotransfected with luciferase reporter and expression plasmids along with Renilla luciferase vector pRL-CMV as an internal control for normalization. The total amount of plasmid DNA was held constantly by the addition of empty plasmid. Cell extracts were prepared with Passive Lysis Buffer (Promega) 48 h after transfection, and the luciferase activities were measured with a Dual-Luciferase assay kit (Promega) using a microplate reader, Infinite M1000 Pro (Tecan), according to the manufacturer's instructions.

Immunoprecipitation assay
Cells were lysed in cell lysis buffer (50 mM Tris-HCl, pH 7.5, buffer containing 150 mM NaCl, 1% Nonidet P-40, and 0.5% deoxycholate) supplemented with the protease inhibitor mixture (Roche Applied Science) at 4°C. After centrifugation at 12,000 ϫ g for 15 min, the supernatants were used for immunoprecipitation with appropriate antibodies coupled to protein G-Sepharose beads (Roche Applied Science). The immunoprecipitants were then washed with cell lysis buffer and subjected to immunoblot analysis. The input represents 10% of the supernatant used in the co-immunoprecipitation experiments.

Real-time quantitative PCR (qPCR)
Total RNA was extracted from cells or midbrain tissues using TRIzol reagent (Invitrogen). Five hundred ng of each RNA sample was reverse-transcribed into cDNA for PCR assays with a PrimeScript RT Master Mix (Takara). Real-time PCR analysis