Structural basis for ADP-dependent glucokinase inhibition by 8-bromo–substituted adenosine nucleotide

In higher eukaryotes, several ATP-utilizing enzymes known as hexokinases activate glucose in the glycolysis pathway by phosphorylation to glucose 6-phosphate. In contrast to canonical hexokinases, which use ATP, ADP-dependent glucokinase (ADPGK) catalyzes noncanonical phosphorylation of glucose to glucose 6-phosphate using ADP as a phosphate donor. Initially discovered in Archaea, the human homolog of ADPGK was described only recently. ADPGK's involvement in modified bioenergetics of activated T cells has been postulated, and elevated ADPGK expression has been reported in various cancer tissues. However, the physiological role of ADPGK is still poorly understood, and effective ADPGK inhibitors still await discovery. Here, we show that 8-bromo–substituted adenosine nucleotide inhibits human ADPGK. By solving the crystal structure of archaeal ADPGK in complex with 8-bromoadenosine phosphate (8-Br-AMP) at 1.81 Å resolution, we identified the mechanism of inhibition. We observed that 8-Br-AMP is a competitive inhibitor of ADPGK and that the bromine substitution induces marked structural changes within the protein's active site by engaging crucial catalytic residues. The results obtained using the Jurkat model of activated human T cells suggest its moderate activity in a cellular setting. We propose that our structural insights provide a critical basis for rational development of novel ADPGK inhibitors.

In higher eukaryotes, several ATP-utilizing enzymes known as hexokinases activate glucose in the glycolysis pathway by phosphorylation to glucose 6-phosphate. In contrast to canonical hexokinases, which use ATP, ADP-dependent glucokinase (ADPGK) catalyzes noncanonical phosphorylation of glucose to glucose 6-phosphate using ADP as a phosphate donor. Initially discovered in Archaea, the human homolog of ADPGK was described only recently. ADPGK's involvement in modified bioenergetics of activated T cells has been postulated, and elevated ADPGK expression has been reported in various cancer tissues. However, the physiological role of ADPGK is still poorly understood, and effective ADPGK inhibitors still await discovery. Here, we show that 8-bromo-substituted adenosine nucleotide inhibits human ADPGK. By solving the crystal structure of archaeal ADPGK in complex with 8-bromoadenosine phosphate (8-Br-AMP) at 1.81 Å resolution, we identified the mechanism of inhibition. We observed that 8-Br-AMP is a competitive inhibitor of ADPGK and that the bromine substitution induces marked structural changes within the protein's active site by engaging crucial catalytic residues. The results obtained using the Jurkat model of activated human T cells suggest its moderate activity in a cellular setting. We propose that our structural insights provide a critical basis for rational development of novel ADPGK inhibitors.
Glycolysis is a central metabolic process in all living cells. It developed early in evolution and is conserved in almost all known organisms. In general, glycolysis generates energy (ATP) by decomposing glucose to pyruvate. In the presence of oxygen, most mammalian cells strongly reduce glycolytic flux to favor mitochondrial ATP generation by oxidation of pyruvate to CO 2 and H 2 O. Aerobic glycolysis, i.e. conversion of glucose-derived pyruvate to lactic acid in the presence of oxygen, was first described by Otto Warburg and is now broadly known as the "Warburg effect" (1). Increased aerobic glycolysis is a characteristic trait of rapidly proliferating cancer cells but also nontransformed tissues, such as activated immune cells (T cells, B cells, or myeloid cells) (2).
Glycolysis begins with the activation of glucose by phosphorylation to glucose 6-phosphate. In higher eukaryotes, this step is catalyzed by several tissue type-specific isoforms of ATP-utilizing enzymes known as hexokinases (HKs; 4 isoforms I to IV in human) (3). An alternative glycolytic pathway involving ADP-dependent glucokinase (ADPGK) (EC 2.7.1.147) was first described in hyperthermophilic Archaea (4), but ADPGK homologs were subsequently discovered and functionally characterized in mouse (mADPGK) (3) and human (hADPGK) (5,6). ADPGKs are structurally unrelated to HKs and constitute distant homologs of family B of sugar kinases within the ribokinase superfamily (7). The physiological significance of a unique ability of ADPGKs to use ADP instead of ATP as a phosphate donor for glucose 6-phosphate generation remains unknown.
Genome-wide expression profiling data of various human and murine tissues indicate that ADPGK is preferentially expressed in hematopoietic lineage, i.e. macrophages, monocytes, dendritic cells, and T and B cells (8 -11). It has been recently demonstrated that ADPGK activity is rapidly and transiently triggered in human T cells upon activation (stimulation of T cell receptor (TCR)) or treatment with phorbol 12-myristate 13-acetate (PMA), a pharmacological mimic of TCR-derived diacylglycerol (DAG)) (10). Elevated ADPGK activity temporarily coincides with TCR-induced glucose uptake and with mitochondrial respiration decrease and thus with a meta- 4 The abbreviations used are: HK, hexokinase; ADPGK, ADP-dependent glucokinase; mADPGK, mouse, ADPGK; hADPGK, human ADPGK; TCR, T cell receptor; DAG, diacylglycerol; PMA, phorbol 12-myristate 13- cro ARTICLE bolic shift toward aerobic glycolysis similar to the Warburg effect. Importantly, enhanced ADPGK activity contributes to the generation of the mitochondrial "oxidative signal," a TCRinduced transient increase in mitochondrial reactive oxygen species (ROS) production. ROS, in turn, facilitate activation of transcription factors, including NF-B, AP-1, and nuclear factor of activated T cells, which results in T cell activationinduced gene expression (10,12,13).
Interestingly, ADPGK is highly expressed in tumor tissues (e.g. lymphocytic leukemia, myeloid leukemia, multiple myeloma, and prostate and breast cancers) (5,8,14,15), whereas mutated ADPGK peptides were found as tumorderived neoantigens inducing strong anti-tumor immune response (16). ADPGK was also postulated to replace hexokinase function under hypoxic conditions (3,5,17), because priming glycolysis with ADP instead of ATP might increase the available ATP pool and thus support cellular survival under hypoxia. Nevertheless, this assumption still awaits experimental confirmation.
Although the specific role of ADPGK in cellular metabolism requires further elucidation, its critical function in T cell activation makes ADPGK an attractive target for the development of immunomodulatory compounds. Nucleotide analogs have demonstrated their value as clinically relevant inhibitors of various nucleotide-binding proteins in hematological malignancies (18). Here, we identified a modified adenosine nucleotide, 8-bromoadenosine monophosphate (8-Br-AMP), as an inhibi-tor of ADPGK activity. By solving a high-resolution crystal structure of ADPGK in complex with 8-Br-AMP, we defined a mechanistic basis for its inhibitory action. We also demonstrate that 8-Br-AMP blocks T cell activation-induced ROS generation (the oxidative signal) and thus ROS-dependent gene expression without significantly influencing the overall glycolytic flux. Our research provides a solid basis for the rational development of specific and more potent ADPGK inhibitors, compounds which are likely to show significant immunomodulatory activity.

Biochemical characterization of hADPGK and identification of inhibitors
hADPGK was expressed using an Escherichia coli recombinant system, and basic enzymatic properties were characterized. ADPGK activity was monitored as the rate of glucose 6-phosphate formation measured in a coupled reaction with glucose-6-phosphate dehydrogenase, as described previously (3,5,10). The pH optimum for the hADPGK-driven reaction was found to be in the physiological range ( Fig. S1A) (5), and all other experiments were performed at pH 7.4. In our hands, the K m values of hADPGK for ADP and glucose were 0.12 Ϯ 0.01 and 0.15 Ϯ 0.025 mM, respectively (Fig. 1, A and B), thus in the range of previously reported values (0.56 mM for ADP and 0.48 mM for glucose (6)). We also tested the ADPGK specificity

ADPGK inhibition by 8-Br-AMP
toward nucleoside diphosphates other than ADP and established that only GDP could serve as an alternative ADPGK substrate, but with much lower efficiency compared with ADP. The specific activity of ADPGK with GDP as a substrate was only 6% of that determined under the same conditions in the presence of ADP as a phosphate donor (Fig. S1B). We also evaluated the possible product inhibition. Under the experimental conditions, the initial activity of hADPGK in the presence of AMP was ϳ85% of the maximal enzymatic activity; however, this slight decrease was independent of AMP concentration up to 5 mM (Fig. 1C) allowing us to conclude that no significant product inhibition was associated with AMP at levels far exceeding the K m values for both substrates.
To identify ADPGK inhibitors, several adenosine analogs were tested for their ability to reduce the recombinant hADPGK activity (Fig. 1C and Table 1, and Table S1). First, we evaluated adenosine and 5-O-acetyladenosine for potential product-like inhibition, but none affected the activity of ADPGK. Interestingly, 8-Br-AMP at 1 mM concentration inhibited more than 70% of the initial hADPGK activity. We thus further focused on 8-Br-AMP and performed a limited structure-activity relationship evaluation by testing the activity of other 8-bromo-substituted adenosine analogs, 8-bromoadenine, 8-bromoadenosine, and 8-bromoadenosine acetate. None of the tested analogs of 8-Br-AMP reduced the activity of hADPGK (even at 5 mM concentration) demonstrating the importance of the ␣-phosphate group in 8-bromo-substituted adenosine binding (Fig. 1C). Our data demonstrate that bromine substitution is essential for hADPGK inhibition (compare AMP and 8-Br-AMP). However, it is only effective when adenosine is phosphorylated (compare 8-Br-AMP and 8-Bradenosine). Further evaluation demonstrated that 8-Br-AMP inhibition has a competitive nature ( Fig. 1D and Fig. S2) and is characterized by K i of 270 Ϯ 30 M.

Crystal structure of apo phADPGK
To uncover the structural determinants of ADPGK inhibition by bromine-substituted nucleotide analogs, we attempted to crystallize hADPGK in complex with 8-Br-AMP. Despite significant efforts to crystallize hADPGK in apo-or inhibitorbound forms (including testing of a large number of crystallization conditions, protein variants, and mutants), our trials remained unsuccessful. Therefore, we cloned and expressed a homolog of hADPGK from the hyperthermophilic archaeon Pyrococcus horikoshii (phADPGK). To use phADPGK as a structural surrogate for hADPGK, we thus verified whether 8-Br-AMP is a phADPGK inhibitor, and we have performed similar kinetic studies as those conducted for human homolog. The K m values of phADPGK for ADP and glucose were 0.13 Ϯ 0.025 and 0.64 Ϯ 0.1 mM, respectively (Fig. S3), and similarly as for hADPGK, 8-Br-AMP competitively inhibits phADPGK with K i of 160 Ϯ 16 M (Fig. S4).
phADPGK crystallized readily, which allowed solving the structures of both the apo-form and of the protein in complex with glucose and 8-Br-AMP. These structures are informative regarding the mechanism of inhibition of not only phADPGK but also hADPGK, because both proteins share significant sequence homology at the active site (Fig. S5).
Initial molecular replacement to solve the structure of apo phADPGK using the previously reported P. horikoshii ADPGK structure (PDB code 1L2L; P2 1 2 1 2 1 space group) (19) was unsuccessful suggesting structural differences within the enzyme in the two different crystal forms. The structure was thus solved by molecular replacement using a large domain of phADPGK (1L2L) and refined to R work /R free values of 0.168/ 0.221 at 2.0 Å resolution in P3 2 21 space group ( Table 2). The asymmetric unit contains a single phADPGK molecule. Residues 4 -457 are well-defined by electron density, including two previously unresolved surface loops (residues 157-162 and 171-174, Fig. 2A). However, the nucleotide-binding loop (residues 435-439) was not defined by electron density in our structure. This suggests multiple conformations of this fragment, and it was not included in the final model. phADPGK belongs to the class of ␣/␤ (a/b) proteins and adopts the ribokinase-like fold. The topology of the protein is identical to that described previously based on the P2 1 2 1 2 1 structure (19) and consists of a large ␣/␤ domain containing 13 ␣-helices, 11 ␤-strands, and three 3 10 helices and a small domain with four ␣-helices and seven ␤-strands. The small domain is composed of helices ␣2-␣5 as well as strands ␤2-␤4 and ␤8 -␤11, whereas the remaining secondary structure elements form the large domain. The link between the domains is composed of three extended stretches of the polypeptide chain (Fig. 2).
Despite the overall architectural similarity between our structure and the structure of phADPGK solved previously in the space group P2 1 2 1 2 1 , the respective orientation of the small and large domains differs between the structures. The calculated r.m.s.d. is 1.742 Å for the whole molecules (0.179 and 0.847 Å for the small and large domains respectively). This reorientation explains the difficulties encountered in molecular

ADPGK inhibition by 8-Br-AMP
replacement using the whole-protein structure as a search model.

Crystal structure of phADPGK-8-Br-AMP-glucose complex
The structure of phADPGK-8-Br-AMP-glucose ternary complex was determined by molecular replacement using the same search model as for the apoprotein. The structure was refined to R work /R free values of 0.16/0.20 at 1.81 Å resolution in space group C2 ( Table 2). The asymmetric unit contains a single phADPGK molecule. The protein model encompasses residues 5-457, all of which are unambiguously defined by the electron density. The structure has 8-Br-AMP and glucose bound at the active site. The nucleotide-binding loop (435-439), which was disordered in the apo structure, is stabilized in the ligand-containing complex and well-defined by the electron density.
Both small molecule ligands (8-Br-AMP and glucose) were visible in the electron density even before their introduction into the model, which was done at the late stages of refinement. Final electron density maps unambiguously define the conformations and the binding pockets for both ligands (Fig. 2, C and D, and Fig. S6A).
8-Br-AMP is located at the nucleotide-binding site between the small and the large domains in the proximity of the nucleotide-binding loop, which isolates the binding pocket from the solvent (Fig. 2C). A prominent hydrophobic surface, which provides significant interactions to the adenosine moiety of 8-Br-AMP, is composed of residues Ala-376, Lys-430, Val-431, and Pro-435. Moreover, residues Thr-346, Ile-441, and Ile-445 are responsible for further hydrophobic contacts with the ribose moiety. The inhibitor is also stabilized by multiple hydrogen bonds (Fig. 2C). The amine group at position 6 of the adenine ring donates hydrogen bonds to the side-chain carboxyl of Glu-198 and main-chain carbonyl of Val-432. The amine at position 1 contributes a hydrogen bond with the Val-432 backbone amide. The bromine atom at position 8 is involved in water molecule-mediated interactions with Tyr-347 hydroxyl, Asn-199 side-chain amide, Arg-200 guanidine group, and ␣-phosphate of 8-Br-AMP. The ribose moiety is stabilized by an extensive network of water molecules mediating interactions with the side chains of Glu-296 and Thr-429 and the main-chain amide of Gly-348. The phosphate group contributes a number of hydrogen bonds to the side chains of Asn-295 and His-345 and water molecule-mediated interactions with side-chain carboxyls of Glu-296 and Glu-298, main-chain amide of Gly-442, Tyr-347 hydroxyl, and the main-chain carboxyl of Thr-438. The binding of the phosphate group is further stabilized by interaction with the guanidine moiety of Arg-200.
The glucose moiety is bound in a groove located at the hinge region between the large and small domains. The binding site is composed of a number of residues assembling an amphipathic pocket (Asp-35, Asp-37, Glu-91, Gly-114, Gly-115, Gln-116, Ile-119, His-179, Ile-181, Ile-202, Asp-443, and Val-439). Glucose is stabilized by hydrogen bond interactions contributed by the side chains of Asp-37, Glu-91, and His-179 as well as the Gly-115 backbone amide. Prominent hydrophobic interactions with glycine residues within a conserved GG motif (residues 114 -115) further stabilize glucose in its binding pocket. The hydroxyl at position 6 is located exactly at the active site being coordinated by hydrogen bonds contributed by the side chains of Gln-116 and Asp-443 and further bonds mediated by two water molecules. The water molecules occupy the presumed positions of phosphonyl oxygens of the incoming phosphate group providing insight into the mechanism of catalysis (Fig. 2D).
A prominent electron density cloud in a solvent-exposed region of the protein remained unexplained by the completed model containing the above-described ligands. This density unambiguously describes another 8-Br-AMP molecule (Fig.  S6B). The noncanonical binding site is located between small and large domains and partially overlaps with the entrance to the canonical nucleotide-binding site. The inhibitor fits between two hydrophobic residues, namely Trp-82 and Phe-272 (Figs. 2E and 3A). The adenosine moiety is positioned by interaction with Phe-272 side chain and hydrophobic interaction with Trp-82. Additionally, a salt bridge connects the ␣-phosphate group of the inhibitor and the guanidine group of Arg-86. Because the second inhibitor-binding site is located in the proximity of the entrance to the catalytic cleft (Fig. 2F), one could envision an allosteric action of the inhibitors described in this study or alternatively the possible physiological role of the site in participation of substrate/product exchange. To rule out this possibility, we disrupted the binding site by mutating Phe-272 into alanine. No significant difference in the inhibition profile of 8-Br-AMP was observed when phADPGK(F272A) was compared with the WT protein ( Fig. 3B and Fig. S7). The same was true for glucose 6-phosphate production kinetics (Fig. S7). To further assess the 8-Br-AMP binding event and compare the WT and F272A phADPGKs, we performed a label-free microscale thermophoresis (MST)

ADPGK inhibition by 8-Br-AMP
experiment. MST has been previously shown to be of great use for quantification of the biomolecular interactions, and its label-free variant allows us to measure affinities and discriminate binding sites for protein-ligand interaction (20). We have tested the binding of 8-Br-AMP to phADPGK, and its F272A variant and the K d values were determined as 16.85 Ϯ 4.12 and 17.44 Ϯ 3.51 M, respectively (Fig. 3C). The measured affinities were corresponding to those of hADPGK, where 8-Br-AMP binds with a K d value of 16.55 Ϯ 4.78 M (Fig. S8). These results advocate for an insignificant role of the second inhibitor-binding site, and they suggest that binding of the second 8-Br-AMP molecule may be the result of high inhibitor concentration applied under the crystallization condition.

8-Br-AMP binding induces major conformational changes
The previously reported structure of apo phADPGK (PDB code 1L2L) was found in an extended open conformation, unusual for this class of proteins. Our structure of apo phADPGK demonstrates a more closed conformation, similar to other apo ADPGK structures reported to date. The small domain is shifted toward the large domain by 8°in our structure compared with a previous structure (PDB code 1L2L). This shift corresponds to the relative domain movement of 8 Å on average ( Fig. 2A). The structure of phADPGK-8-Br-AMPglucose complex demonstrates even more compact conformation that we will term "closed" in further discussions. Comparison with our apoprotein structure revealed the relative shift of the small domain toward the large domain by 11°, which translates into an average 9 Å of relative domain movement (Fig. 2B).
Binding of the inhibitor seems to promote the closed conformation of the protein. To determine whether this is related to the particular inhibitor utilized in our study or rather a general mechanism related to the substrate/substrate analog binding and catalysis in general, we compared our structure to that of ADPGK from Thermococcus litoralis (tlADPGK) in complex with AMP (21). Both structures are found in a closed confor-

ADPGK inhibition by 8-Br-AMP
mation; however, the domain arrangement is tighter in the phADPGK-8-Br-AMP-glucose complex. This more compact protein architecture is reflected by a relative shift of a small domain toward a large domain by 6°, which corresponds to the relative domain movement of 4 Å (Fig. 3A), when the structures of phADPGK and tlADPGK are compared. It remains to be

ADPGK inhibition by 8-Br-AMP
determined whether this difference is related to the bromine substituent present in our inhibitor or to a general difference between analyzed proteins.
The amino acid residues comprising the hydrophobic surface within the nucleotide-binding site found in the tlADPGK structure are almost identical as those of phADPGK, and the only difference is the substitution of Val-431 by Ile-439 in the case of tlADPGK. This allows deciphering the contribution of bromine substitution in 8-Br-AMP binding by comparing the binding modes within the two structures. No prominent differences between AMP and 8-Br-AMP hydrophobic interaction pattern were observed. However, the inhibitor binding still induces major conformational changes within the active site as compared with the AMP-containing structure, all of which are related to polar interactions (Fig. 3D). The most notable rearrangement involves a Glu-Asn-Arg motif (residues 198 -200 and 203-205 in phADPGK and tlADPGK, respectively) within which the main chain shifts toward the inhibitor molecule by 1-2 Å compared with the structure containing AMP. The hydrogen bond between the side chain of Glu-198 and the amine group at position 6 of the 8-Br-AMP adenine ring is not present in the AMP-containing structure. Moreover, the bromine moiety induces a 3-4 Å shift in side chains of Asn-199 and Arg-200 in the direction of the ␣-phosphate compared with the AMP-containing structure (Fig. 3D). Remarkably, the single water molecule mediates interactions between bromine moiety with side chains of Asn-199 and Arg-200 as well as with ␣-phosphate. Further differences between AMP and 8-Br-AMP structures involve stabilization of the Tyr-347 side chain, which was found in two alternative conformations in tlADPGK but is rigid in the structure containing 8-Br-AMP. This stabilization is also induced by a water-mediated interaction of Tyr-347 sidechain hydroxyl and the bromine atom.
Next, the Glu-296 side chain, which does not interact with AMP in the tlADPGK structure, shifts toward the inhibitor molecule and forms a water-mediated interaction with the O3 atom of the ribose moiety, Tyr-347 side chain, and the ␣-phosphate. To further assess the impact of these interactions, we designed several phADPGK mutants. The substitutions of Arg-200 and Glu-298 for alanine led to inactivation of phADPGK. Remarkably, the substitution of Tyr-347 did not cause any significant changes either in the protein activity or inhibition by 8-Br-AMP (Fig. S9).
Taken together, the bromine atom engages a number of interactions essential for the described side-chain rearrangements. The majority of 8-Br-AMP-induced changes within the active site involve, directly or indirectly (via water molecules), the ␣-phosphate group. This observation provides hints why only a hydrogen bond-rich moiety (phosphonate) is accepted at that position of the inhibitor, whereas analogs not able to form these important interactions (8-bromoadenosine and 8-bromoadenosine acetate) display no inhibitory activity (Fig. 1D).

8-Br-AMP inhibits T cell activation-induced ROS release and gene expression
Activation of ADPGK in human T cells is an important step among metabolic/signaling events in TCR-induced mitochondrial ROS release (oxidative signal generation) and conse-quently ROS-dependent gene expression (10 -12). Using a well-established model of PMA (DAG)-mediated activation of the human Jurkat T cell line, we tested the effect of 8-Br-AMP on these cellular events. Treatment of Jurkat T cells with 8-Br-AMP, at concentrations which did not result in any noticeable cytotoxicity (Fig. S10C), dose-dependently reduced the oxidative signal generation and, consequently, diminished transcription of IL-2 and IB␣. The highest 8-Br-AMP concentration tested (100 M) decreased the PMA-triggered generation of ROS to around half of the initial value (Fig. 4A). Interestingly, the obtained extent of inhibition corresponds to that obtained upon siRNA-or shRNA-mediated ADPGK knockdown (50 -65%) (10). The PMA/ionomycin-induced expression of classical T cell activation-responsive genes, IL-2 and IB␣, was decreased in the presence of 8-Br-AMP (Fig. 4B). Moreover, 8-Br-adenosine, which was ineffective as a hADPGK inhibitor in vitro (Fig. 1D), was also ineffective in decreasing the PMAinduced oxidative signal (Fig. S10A) and PMA/ionomycin-induced downstream gene expression when used at a concentration corresponding with that of 8-Br-AMP (Fig. S10B).
T cell activation-induced mitochondrial ROS release and the resulting gene expression rely on TCR (PMA/DAG)-mediated, transient up-regulation of glucose metabolism (10). Because Br-AMP was reported to inhibit AMPK activity (22)(23)(24) and bind to HKs (25), it could have a significant influence on the overall glucose metabolism. To evaluate the influence of 8-Br-AMP on the overall glucose flux of proliferating Jurkat T cells, we employed the "glycolysis stress test" method and Seahorse extracellular flux analyzer. 8-Br-AMP at 100 M only slightly decreased the basal (determined after addition of glucose) and the maximal (determined in the presence of oligomycin) glycolytic flux indicating no significant influence of the inhibitor on the general glucose metabolism (Fig. 4C).

Discussion
Development of inhibitory strategies against highly glycolytic metabolism of activated immune cells or proliferating cancer cells as well as against T cell activation-induced release of pro-inflammatory cytokines may have a great impact on autoimmunity or malignancies. Although the general role of ADPGK in cellular metabolism awaits characterization, the involvement of TCR (DAG)-induced ADPGK activity in the metabolic shift toward aerobic glycolysis of activated T cells and in the regulation of T cell function makes ADPGK a putative immunomodulatory target (10). We report here that the structural basis of ADPGK inhibition by 8-bromo-substituted adenosine nucleotide constitutes the first step for pharmacologically relevant inhibitor identification.

ADPGK inhibition by 8-Br-AMP
(HINT, PDB code 5RHN) (29). Because of its promiscuity, 8-Br-AMP by itself is an unlikely starting point for direct inhibitor optimization, but it serves well to define the pharmacophore and the binding pocket for virtual screening.
8-Br-AMP is a competitive inhibitor of ADPGK, but the bromine substitution induces prominent changes within the protein-active site by engaging crucial catalytic residues. In particular, the side-chain position of the conserved arginine residue (Arg-200) is affected. During glucose phosphorylation, Arg-200 is responsible for the positioning of ␤-phosphate and stimulates the phosphate transfer from ADP (21, 30, 31). In our structure, the Arg-200 side chain is involved in water-mediated interaction with the bromine and shifts toward ␣-phosphate of 8-Br-AMP compared with the AMP-containing structure (Fig. 3D). This induced disposition may be utilized in the rational design of more potent and selective inhibitors.
We identified a second inhibitor binding site outside of the ADP-binding pocket. Secondary sites are relatively frequent crystallization artifacts related to high concentrations of ligands utilized in the experiments. Occasionally, however, high-ligand concentrations allow for identifying weak secondary binding sites involved in substrate access/product egress of the binding pocket. Alternatively, secondary sites may be responsible for allosteric regulation (32,33). To evaluate the relevance (if any) of a secondary 8-Br-AMP-binding site, we first analyzed the known structures of ADPGK homologs from T. litoralis and Mus musculus, which indicated little conservancy in the region of interest. tlADPGK has tyrosine in the position equivalent to Phe-272 of phADPGK, whereas mADPGK is characterized by methionine. These are residues of comparable hydrophobic character that might suggest conservation (and thus functionality) of the site. However, mutating the major residue within the site (phADPGK-F272A) had no significant effect on enzyme activity, 8-Br-AMP inhibition, and inhibitor binding. This allowed us to conclude that the additional inhibitor-binding site seen in our structure represents an experimental artifact resulting from high 8-Br-AMP concentration used for protein crystallization.
Prior data demonstrated that Thermococcus ADPGK oscillates between different conformational states (21, 34). The enzyme either adopts an open conformation, which is characteristic for the apoprotein, or a closed conformation seen in a protein containing AMP and glucose at the active site. Strikingly, Ito et al. (34) demonstrated that tlADPGK containing ADP, but in the absence of glucose, is found in the open state. Similarly, binding of AMP does not induce any prominent domain rearrangement in mADPGK (6). This implies that the protein adopts closed conformation only when both substrates occupy the active site (or alternatively, although less likely, that glucose induces the closed conformation). This study adds further complexity to conformational transitions related to ADPGK catalysis/inhibitor binding. The crystal structure of

ADPGK inhibition by 8-Br-AMP
phADPGK reported by Tsuge et al. (19) contains the apoprotein found in an unusually extended conformation. In contrast, the structure of apo phADPGK presented in our work adopts a more compact conformation concerning the relative arrangement of small and large domains ( Fig. 2A). In our second structure, binding of a substrate (glucose) and substrate analog (8-Br-AMP) induces domain closure, which in fact is even tighter than the one described for tlADPGK in the presence of glucose and AMP. This phenomenon coincides with prominent rearrangements of amino acids within the substrate-binding pocket when 8-Br-AMP-and AMP-bound structures are compared (Fig. 3D). Clearly, ADPGK is a dynamic protein present in multiple open conformations in the absence of both ligands to facilitate their recognition. Ligand binding stabilizes the closed conformation capable of catalysis. The same is observed for competitive inhibitors occupying the ADP-binding pocket in the presence of a second substrate.
Prior to this study, the only attempt to determine the crystal structure of ADPGK in complex with the inhibitor was that for tlADPGK (21). tlADPGK was crystallized in the presence of Mg-ADP␤S (PDB code 4B8S), but the electron density was explained by the authors with the AMP molecule, and no density for the ␤-phosphate or magnesium ion was observed, most probably because of the hydrolysis of the inhibitor. Therefore, the structure reflects a product-containing structure of ADPGK in the closed conformation. Upon comparing the tlADPGK-AMP and phADPGK-8-Br-AMP structures, the r.m.s.d. is 1 Å for the whole molecules and 0.62 Å for the large domains only. However, the crevice dimensions between the small and large domain differ significantly between both proteins. Compared with the tlADPGK structure, the small and large domains of phADPGK reported in our study are much closer together, which is reflected by the measured distances between Gly-87 and Ile-316 residues being 11 and 16 Å for phADPGK and tlADPGK, respectively (Fig. 3A). Because the second 8-Br-AMP molecule, which binds phADPGK, is located on the protein surface in the proximity of the described crevice entrance, this interaction could be additionally responsible for a tighter arrangement of large and small domains in the case of phADPGK compared with the tlADPGK-AMP complex.
Our data also demonstrate that 8-Br-AMP treatment downregulates cellular processes that were earlier described to depend on ADPGK activity (10 -12). Using the Jurkat model of human T cell activation, we demonstrated that 8-Br-AMP diminishes DAG/PMA-induced ROS release and therefore ROS-dependent pro-inflammatory gene expression. Because Br-AMP likely acts promiscuously on several molecular targets, including sugar and protein kinases, it is currently unclear whether the inhibitory action is solely ADPGK-dependent. However, it is remarkable that the extent of the inhibitory effect of 8-Br-AMP on ROS generation and ROS-dependent gene expression parallels that previously reported for ADPGK knockdown experiments (10). In addition, corresponding concentrations of 8-Br-AMP induced no significant reduction in the overall glycolytic flux of proliferating Jurkat T cells. This observation seems to preclude the inhibitory effect of tested concentrations of 8-Br-AMP on major metabolic hubs, like ATP-dependent HKs or AMPK. The observation also advo-cates that ADPGK activity is not essential for the overall cellular glucose metabolism. This is consistent with prior studies on ADPGK knockdown, knockout, and overexpression in several cancer cell lines cultured under normoxic, hypoxic, or anoxic conditions (5,17). These conclusions are, however, largely speculative, and further investigation is necessary.
In summary, our study demonstrates that the bromine-substituted AMP analog inhibits ADPGK. However, in the case of the investigation of the role of ADPGK in cellular metabolism and regulation of T cell activation, caution must be taken in the interpretation of the results due to 8-Br-AMP promiscuity. The provided crystal structure explains the binding mode and constitutes a starting point for further inhibitor development.

Molecular cloning and expression
The hADPGK-encoding gene (residues 29 -497) was codonoptimized for the E. coli expression system, synthesized, and cloned into the pET24d plasmid (Genscript). For expression, the gene was subcloned into pGEX-6p-1 plasmid resulting in a construct bearing the N-terminal GST tag followed by the Pre-Scission protease cleavage site. The gene encoding full-length phADPGK (residues 2-457) was synthesized and cloned into pET15b plasmid (Genescript). The final expression construct bearing the N-terminal hexahistidine tag followed by TEV protease recognition site was generated by subcloning into pETM11 vector (EMBL). Site-directed mutagenesis of phADPGK has been performed as described previously (35).
For protein expression, E. coli BL21 (DE3) competent cells were transformed with either pGEX6p1-hADPGK or pETM11-phADPGK plasmids and cultured at 37°C in LB Lenox medium (Bioshop) or Terrific Broth (Bioshop), respectively. When the A 600 reached 0.6, the expression was induced by addition of isopropyl ␤-D-1-thiogalactopyranoside at final concentration of 0.4 mM, and the culture was continued for 20 h at 20°C. Cells were harvested by centrifugation at 4000 ϫ g. Pellets were flashfrozen in liquid nitrogen and stored at Ϫ80°C until further use.

hADPGK purification
Frozen pellets were thawed, resuspended in lysis buffer (50 mM Tris-HCl, pH 8.0, 500 mM NaCl), and lysed by sonication. The lysate was clarified by centrifugation (20 min at 50,000 ϫ g; 4°C), and the protein of interest was recovered using GSH-Sepharose (GE Healthcare). Overnight on-column proteolysis with PreScission protease facilitated the GST-tag removal, and the related hADPGK was further purified by size-exclusion chromatography on Superdex 75 (GE Healthcare) using the same buffer. Purified hADPGK was concentrated to 1 mg/ml and stored at Ϫ80°C for further analysis.

phADPGK purification
Frozen pellets were thawed, resuspended in lysis buffer (20 mM Hepes, pH 8.0, 350 mM NaCl, 10 mM MgCl 2 , 2.5% glycerol, 20 mM imidazole), and lysed by sonication. The lysate was clarified by centrifugation (20 min at 50,000 ϫ g; 4°C), heat shocked at 90°C for 10 min, and again clarified by centrifugation (20 min at 50,000 ϫ g; 4°C). The protein of interest was ADPGK inhibition by 8-Br-AMP recovered by metal affinity chromatography using chelating-Sepharose (GE Healthcare) pre-equilibrated with the lysis buffer. Protein was eluted using imidazole gradient (up to 500 mM). Fractions containing phADPGK were incubated at 4°C with TEV protease (DNA Gdansk) for 12 h. The protein of interest was separated from the released tag and the remaining uncleaved forms by size-exclusion chromatography (Superdex 200, GE Healthcare) in GFB buffer (20 mM Hepes, pH 8.0, 150 mM NaCl, 5 mM MgCl 2 ). Purified phADPGK was either directly used for crystallization trials or concentrated to 1 mg/ml and stored at Ϫ80°C for further analysis.

ADPGK activity assay and inhibitor screening
ADPGK activity was assessed by monitoring the level of one of the reaction products (glucose 6-phosphate) in a coupled glucose-6-phosphate dehydrogenase (G6PD) reaction essentially as described previously (6,10). The reaction was initiated by addition of ADPGK substrates, ADP and D-glucose (0.5 mM), and NADP reduction by G6PD was monitored spectrophotometrically at 340 nm. Activity assays were performed in 96-well format spectrophotometric plates using TECAN microplate UV-visible reader. Unless stated otherwise, the assays were performed at 37°C in reaction buffer (20 mM Tris-HCl, pH 7.4, 250 mM sucrose, 50 mM KCl, 5 mM MgCl 2 ) supplemented with glucose-6-phosphate dehydrogenase (1 unit), NADP (0.5 mM). Potential inhibitors were screened at an initial concentration of 1 mM. K m or K i values were determined by varying glucose, ADP, and inhibitor concentrations, and analysis was done using nonlinear regression and competitive inhibition model using GraphPad Prism 6 software as described previously (6). The 8-bromo-substituted analogs were purchased from Sigma (Germany) except for 8-Br-AMP, which was obtained from Jena Bioscience (Jena, Germany).

Crystallization and structural determination
phADPGK was concentrated up to 10 mg/ml and crystallized using a sitting drop vapor diffusion method. 2-l drops of protein solution containing glucose (5 mM), Hepes (20 mM at pH 8.0), sodium chloride (150 mM), and magnesium chloride (5 mM) were mixed with an equal volume of the reservoir solution containing 15% (w/v) PEG 6000, 0.2 M LiSO 4 , and 0.1 M citrate buffer, pH 3.6. The plates were incubated at 20°C and crystals appeared after 3-5 days.
Crystals of phADPGK-glucose-8-Br-AMP complex were obtained using a similar procedure as those of the apoprotein, except that the protein solution was supplemented with 8-Br-AMP (1 mM) and D-glucose (5 mM) and mixed with an equal volume of the reservoir solution containing ammonium nitrate (0.2 M) and PEG 3350 (20%). Co-crystals of the complex appeared after approximately 1 week.
Crystals were soaked in the reservoir solution supplemented with glycerol (20%) and flash-cooled in liquid nitrogen. Diffraction data were collected on beamline BL14.1 operated by the Helmholtz-Zentrum Berlin (HZB) at the BESSY II (Berlin-Adlershof, Germany) (36). The data were indexed, integrated, scaled, and merged using XDS package (37) with XDSAPP2.0 GUI (38). Molecular replacement was performed in Phaser (39,40) using the large domain of phADPGK (PDB code 1L2L) as a search model. Structures were refined using Phenix (41), and model building was done with Coot (42,43).

Binding analysis by microscale thermophoresis
MonolithNT.LabelFree instrument (NanoTemper Technologies GmbH, Munich, Germany) was used to measure the binding interactions between purified phADPGK or phADPGK F272A and 8-Br-AMP. A range of concentrations of 8Br-AMP from 30.5 nM to 1 mM was incubated for 5 min with each protein variant at a concentration of 500 nM in assay buffer (20 mM Tris-HCl, pH 7.4, 50 mM NaCl, 5 mM MgCl 2 , 2 mM glucose). The samples were loaded into the MonolithNT.LabelFree standard-treated glass capillaries, and initial fluorescence measurement followed by thermophoresis measurement were carried out using 40% LED power and 40% MST power, respectively. All measurements were performed at a fixed temperature of 37°C. K d values were calculated using the MO.Affinity Analysis software. Each experiment was performed in triplicate.

Determination of ROS generation and changes in gene expression and cell death
Jurkat T cells (clone E6-1, human acute T cell leukemia cell line) were cultured at standard conditions in RPMI medium supplemented with 10% FBS, 1 mM glutamine, and penicillin/ streptomycin (10 units/ml). All chemicals were from Sigma, unless stated otherwise. Cells were stained with H 2 DCF-DA (Life Technologies, Inc., 5 M) for 30 min (Ϯ8-Br-AMP), stimulated with PMA (10 ng/ml, 1 h) without inhibitor removal, washed with ice-cold PBS, and analyzed by FACS. Generation of the oxidative signal was calculated as described previously (10,12).
To analyze activation-induced gene expression, Jurkat T cells were pre-treated Ϯ 8-Br-AMP for 30 min and activated with PMA (10 ng/ml) and ionomycin (1 M) for 1 h in the presence/ absence of inhibitor. Next, total cellular RNA was isolated with RNeasy mini kit (Qiagen) and reverse-transcribed. Gene expression was analyzed by SYBR Green monitored RT-PCR with IL-2-and IB␣-specific primers. The measurements were normalized at the actin transcript level. Conditions of reverse transcription and RT-PCR and primer sequences were identical to those reported before (10,12). Cell death was measured by propidium iodide (PI) exclusion method. Cells were stained with PI solution (4 g/ml in PBS) for 30 min, and PIϩ cells were assayed by FACS.

Seahorse measurement of glycolytic flux
Total glycolytic flux was determined as extracellular acidification rate (ECAR) using Seahorse XFe 24 extracellular flux analyzer (Seahorse, Agilent). Jurkat T cells were pre-treated with Ϯ100 M 8-Br-AMP for 30 min. Next, 700 ϫ 10 3 cells/well were plated using Cell Tak cell adhesive (Corning) and glucoseand FBS-free standard Seahorse Dulbecco's modified Eagle's medium supplemented with 2 mM glutamine and 1 mM sodium pyruvate Ϯ 100 M 8-Br-AMP. "Glycolysis stress tests" were performed according to manufacturer's procedure, with serial