Calcium-sensitive pyruvate dehydrogenase phosphatase is required for energy metabolism, growth, differentiation, and infectivity of Trypanosoma cruzi

In vertebrate cells, mitochondrial Ca2+ uptake by the mitochondrial calcium uniporter (MCU) leads to Ca2+-mediated stimulation of an intramitochondrial pyruvate dehydrogenase phosphatase (PDP). This enzyme dephosphorylates serine residues in the E1α subunit of pyruvate dehydrogenase (PDH), thereby activating PDH and resulting in increased ATP production. Although a phosphorylation/dephosphorylation cycle for the E1α subunit of PDH from nonvertebrate organisms has been described, the Ca2+-mediated PDP activation has not been studied. In this work, we investigated the Ca2+ sensitivity of two recombinant PDPs from the protozoan human parasites Trypanosoma cruzi (TcPDP) and T. brucei (TbPDP) and generated a TcPDP-KO cell line to establish TcPDP's role in cell bioenergetics and survival. Moreover, the mitochondrial localization of the TcPDP was studied by CRISPR/Cas9-mediated endogenous tagging. Our results indicate that TcPDP and TbPDP both are Ca2+-sensitive phosphatases. Of note, TcPDP-KO epimastigotes exhibited increased levels of phosphorylated TcPDH, slower growth and lower oxygen consumption rates than control cells, an increased AMP/ATP ratio and autophagy under starvation conditions, and reduced differentiation into infective metacyclic forms. Furthermore, TcPDP-KO trypomastigotes were impaired in infecting cultured host cells. We conclude that TcPDP is a Ca2+-stimulated mitochondrial phosphatase that dephosphorylates TcPDH and is required for normal growth, differentiation, infectivity, and energy metabolism in T. cruzi. Our results support the view that one of the main roles of the MCU is linked to the regulation of intramitochondrial dehydrogenases.

Mitochondrial Ca 2ϩ uptake in vertebrate cells is important for regulating the activity of three mitochondrial dehydrogenases (1). Intramitochondrial Ca 2ϩ stimulates pyruvate, 2-oxoglutarate, and isocitrate dehydrogenases, leading to stimulation of oxidative phosphorylation and ATP production (2).
The pyruvate dehydrogenase complex (PDC) 5 catalyzes the conversion of pyruvate to acetyl-CoA and links glycolysis with several pathways such as the Krebs cycle and the synthesis of fatty acids and cholesterol. PDC is a multienzyme complex consisting of multiple copies of E1 (subunits ␣ and ␤, pyruvate dehydrogenase), E2 (dihydrolipoyl transacetylase), and E3 (dihydrolipoyl dehydrogenase) subunits, along with an E3binding protein (3). In vertebrate cells the PDC is regulated through the interconversion of phosphorylated and dephosphorylated forms (4). Inactivation of PDC results by phosphorylation of three serine residues on the ␣ chain of the E1 subunit named sites 1 (Ser-264), 2 (Ser-271), and 3 (Ser-203) (numbers correspond to the mature human enzyme) (5,6). This phosphorylation is catalyzed by a pyruvate dehydrogenase kinase (PDK) whereas their dephosphorylation and reactivation, which is stimulated by Ca 2ϩ , is catalyzed by a pyruvate dehydrogenase phosphatase (PDP). Of the two PDP catalytic subunits present in mammalian mitochondria, PDP1c is the one activated by Ca 2ϩ (7).
There is evidence that the PDC is regulated by phosphorylation in yeast, plants, and invertebrates (8 -11) but their sensitivity to Ca 2ϩ is unknown (2). Early work suggested that the acquisition of Ca 2ϩ sensitivity by mitochondrial dehydrogenases was linked to the acquisition of a mitochondrial Ca 2ϩ uniporter (MCU) (1,12).
The finding of MCU activity in trypanosomatids, such as Trypanosoma cruzi (13,14), the agent of Chagas disease, and T. brucei (15), which belongs to the group of parasites that cause sleeping sickness, was important for the discovery of a gene encoding a modulator of the uniporter (mitochondrial calcium uptake 1, or MICU1) (16) and the pore subunit of the channel or MCU (17)(18)(19). After the molecular identification of MCU, other components of the MCU complex were described (20 -23), but only orthologs of MCU, MCUb, MICU1, and MICU2 are present in trypanosomes, whereas MCU regulator (MCUR1) and essential MCU regulator (EMRE) are absent (24,25). Mitochondrial Ca 2ϩ transport in trypanosomes is critical for shaping the dynamics of cytosolic Ca 2ϩ increases, for the bioenergetics of the cells, and for viability and infectivity (26,27). Interestingly, the PDH E1␣ subunit of trypanosomes exhibits the putative phosphorylation sites present in the mammalian enzyme (28). This enzyme is active in all stages of T. cruzi (29) and T. brucei (26,30) and our previous results (26) suggested that Ca 2ϩ could stimulate it, although a direct stimulation was not demonstrated.
In this study, we report the identification of the mitochondrial PDP of T. cruzi (TcPDP) and T. brucei (TbPDP), demonstrate their activation by Ca 2ϩ , and evaluate the function of the T. cruzi enzyme in growth, differentiation, infectivity, and bioenergetics by CRISPR/Cas9-driven ablation of its gene. The results provide evidence of the presence of a Ca 2ϩ -activated PDP in organisms other than vertebrates and support the view (1) that one of the main roles of the mitochondrial Ca 2ϩ uniporter is linked to the regulation of intramitochondrial dehydrogenases.

Sequence analysis
Putative T. cruzi pyruvate dehydrogenase phosphatase (TcPDP) is a 415-aa protein with an expected size of 46 kDa encoded by a single copy gene (TcCLB.506739.200). The gene is located on chromosome 3 of T. cruzi CL Brener Esmeraldo-like genome sequence (curated reference strain, TriTrypDB) (31). This is the gene ortholog of T. brucei putative protein phosphatase 2C (PP2C, gene ID: Tb427.05.1660) that has been proposed to be the specific phosphatase of TbPDH E1␣ subunit (TbPDP) in a proteomic study using stable isotope labeling of amino acids in cell culture (SILAC) in combination with MS (32). TbPDP is a homolog of the PDH-activating enzyme in other systems and is up-regulated at the protein level upon transition to the insect form (32). TbPDP and TcPDP belong to the PP2C/ PPM family, which is a group of Mg 2ϩ /Mn 2ϩ -dependent serine/threonine phosphatases essential for regulation of cell cycle and stress signaling pathways in different cell types (Inter-Pro protein sequence analysis and classification) (33). Both enzymes exhibit 31% identity with human PDP catalytic subunit 1 at the amino acid level. Using ScanProsite tool (34) for detection of signature matches on TcPDP amino acid sequence we found a PPM-type phosphatase domain (aa 143-414). This type of domain is also present in TbPDP (aa 155-424) and in the catalytic subunit of the mammalian Ca 2ϩ -sensitive PDP1c (35).

TcPDP localization
In eukaryotic cells, pyruvate dehydrogenase is a conserved enzyme that catalyzes the decarboxylation of pyruvate into acetyl-CoA, a reaction that takes place in the mitochondrial matrix. To determine the cellular localization of putative Shown is an amino acid sequence comparison of PDH E1␣ orthologs across several species, where putative phosphorylation sites 1 (blue), 2 (pink), and 3 (orange) are highlighted. Sequence of the phosphopeptides used as substrates in PDP activity assays are underlined in T. cruzi and T. brucei orthologs. Accession numbers: Homo sapiens (NP_000275.1), Bos taurus (NP_001094516.1), Xenopus laevis (AAI06671.1), Drosophila melanogaster (NP_726946.1), Caenorhabditis elegans (NP_871953.1), Dictyostelium discoideum (XP_629349.1), T. cruzi (XP_814071.1), and T. brucei (XP_823475.1). Protein alignment was performed using Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/) (81) (Please note that the JBC is not responsible for the long-term archiving and maintenance of this site or any other third party hosted site.). Asterisk indicates positions which have a single, fully conserved residue; colon indicates conservation between groups of strongly similar properties (Clustal scoring Ͼ0.5); period indicates conservation between groups of weakly similar properties (Clustal scoring Յ0.5).

The role of pyruvate dehydrogenase phosphatase in T. cruzi
TcPDP we generated a mutant cell line that overexpresses a tagged version of the protein (TcPDP-3xHA) by cloning the PCR-amplified ORF of the gene into pTREX-n/3xHA vector (38). After selection, G418-resistant cells were analyzed by Western blotting and immunofluorescence analyses (IFA) using monoclonal anti-HA antibodies (Fig. 2, A and B). Fluorescence microscopy images showed TcPDP localized to mitochondria, as observed by partial co-localization with the mitochondrial marker MitoTracker (Fig. 2B).
We have observed previously that protein overexpression can lead to mislocalization of proteins (39). Therefore, to obtain an accurate localization pattern we used CRISPR/Cas9 system to generate an endogenously tagged mutant cell line (TcPDP-3xc-Myc), as described previously (39,40). TcPDP tagging was confirmed by PCR (Fig. 2, C and D) and also by Western blotting and IFA using monoclonal anti-c-Myc antibodies (Fig. 2, E and F).
Our results indicate that TcPDP is a mitochondrial enzyme, as shown by co-localization with MitoTracker (Fig. 2F, merged image).

Calcium sensitivity of PDP in trypanosomes
None of the dehydrogenases stimulated by Ca 2ϩ in vertebrates has been studied in detail in trypanosomes. However, the PDH E1␣ subunit of T. cruzi and T. brucei possesses putative phosphorylation sites that are similar to those of the mammalian homologs (28), suggesting that, as the mammalian enzyme, they could be activated by Ca 2ϩ -stimulated dephosphorylation. To further investigate the role of mitochondrial putative TcPDP and TbPDP we induced their heterologous expression in Escherichia coli and assayed their activity under different free Ca 2ϩ concentrations. Gene cloning, protein expression, and purification under native conditions were performed as described under "Experimental Procedures." Both recombinant proteins were expressed as fusion proteins with an N-terminal polyhistidine tag. In Fig. 3, A and C, we analyzed different fractions from TcPDP and TbPDP protein purification by SDS-PAGE on 10% gels. In each image, the band corresponding to the recombinant protein is visualized in the total lysate (Lys), but is absent in the flow through fraction (FT), and then the purified protein is observed in the eluted fraction as a single band (TcPDP and TbPDP lanes). Both proteins exhibit molecular weights close to their expected sizes (63 kDa and 64 kDa, respectively), according to the protein molecular weight standard (MW). Purified recombinant PDPs were quantified and used for enzymatic assays. PDP activity was assayed by measur- Figure 2. TcPDP localization. A, TcPDP-3xHA overexpression was confirmed by Western blot analysis using anti-HA monoclonal antibodies, with pTREX empty vector as control cell line. Tubulin (Tub) was used as loading control. B, IFA showed partial co-localization between TcPDP-3xHA (green) and MitoTracker (MT, red) in the merged image (yellow). C, schematic representation of CRISPR/Cas9-mediated TcPDP endogenous tagging. Asterisks indicate stop codons. Vertical arrow indicates Cas9 cut site at the 3Ј end of the gene. Horizontal arrows represent annealing sites for primers used to verify TcPDP endogenous tagging by PCR analysis shown in D. D, lanes on 1% agarose gel: L, 1 kb plus ladder; Scr, control cells transfected with scramble sgRNA; Myc, TcPDP-3xc-Myc; C, PCR negative control. E and F, TcPDP endogenous tagging was verified by Western blotting and IFA using antibodies anti-c-Myc. Merged image on F shows co-localization (yellow) of TcPDP-3xc-Myc (green) and MT (red). DAPI staining (blue) and differential interference contrast (DIC) images are also shown. Scale bars ϭ 10 m.

The role of pyruvate dehydrogenase phosphatase in T. cruzi
ing phosphate release from a synthetic phosphopeptide from either TcPDH or TbPDH E1␣ subunits containing the phosphorylated sites 1 and 2 that regulate PDH activity (41), as described in "Experimental Procedures." A colorimetric assay with malachite green/ammonium molybdate solution allowed the quantification of total P i released during substrate dephosphorylation under different Ca 2ϩ concentrations. Our results indicate that TcPDP and TbPDP exhibit maximal activity at 100 nM Ca 2ϩ and 1 M Ca 2ϩ , respectively (Fig. 3, B and D), which suggests a physiological Ca 2ϩ response in vivo for the putative phosphatases that activate the mitochondrial pyruvate dehydrogenases of T. cruzi and T. brucei.

Enzyme kinetics of recombinant TcPDP
Free Ca 2ϩ concentration found to render optimal TcPDP activity (100 nM) was used to assay the enzyme kinetics at different substrate concentrations as described under "Experimental Procedures." TcPDP kinetic parameters were determined by linear regression using the Michaelis-Menten equation (Fig. 3E). Recombinant TcPDP exhibits a V max of The role of pyruvate dehydrogenase phosphatase in T. cruzi 5.75 Ϯ 0.1 pmol min Ϫ1 mg Ϫ1 , a K m of 32.23 Ϯ 2.04 M, and a catalytic efficiency (k cat /K m ) of 2.9 ϫ 10 3 s Ϫ1 M Ϫ1 .

TcPDP knockout phenotype
To further investigate the role of putative TcPDP we generated a TcPDP knockout (TcPDP-KO) cell line using the CRISPR/Cas9 system we have adapted and successfully used in T. cruzi (38,42). Using this methodology a DNA donor template is provided to promote double-strand break repair by homologous recombination and therefore gene replacement with a resistance marker (the gene encoding blasticidin-S deaminase, Bsd) at the genomic locus ( Fig. 4A) (43). We generated a molecular construct for the constitutive expression of Cas9 and a specific sgRNA targeting TcPDP gene of T. cruzi, as described The role of pyruvate dehydrogenase phosphatase in T. cruzi under "Experimental Procedures." Alternatively, we generated a version of this construct containing an enhanced Cas9 (eSpCas9) with nuclear localization signal and fused to GFP (44), cloned in pTREX-n vector. Each plasmid was co-transfected with the DNA donor cassette into T. cruzi Y strain epimastigotes. After 5 weeks of selection, genomic DNA from double-resistant parasites was analyzed by PCR using a primer set designed to discriminate between the intact TcPDP locus and the one replaced by Bsd (Fig. 4B). PCR products were resolved in a 1% agarose gel and a band of 1608 bp was detected in the control cell line transfected with a scrambled sgRNA (Scr) corresponding to the intact TcPDP locus (Fig. 4C). A single band corresponding to the size of the replaced gene (759 bp) was amplified in the transfectant cell line obtained with regular Cas9, indicating the generation of a homogeneous knockout population where both TcPDP alleles were ablated. Interestingly, using the eSpCas9 enhanced endonuclease, a single knockout (SKO) cell line was generated, as indicated by the amplification of both bands with the same intensity from its gDNA (Fig. 4C). Subsequently, we obtained clonal populations by serial dilutions from this cell line and confirmed the replacement of only one TcPDP allele in these clones (data not shown). The absence of TcPDP in the TcPDP-KO cell line was confirmed by sequencing and Southern blot analysis (Fig. 4, A and D), and then we proceeded to evaluate the phenotype of this mutant. We first analyzed the level of PDH phosphorylation in these parasites by Western blotting using commercial antibodies anti-PDH E1␣ subunit (phosphopeptide Ser-293) of human cells, which recognizes the phosphorylated site 1 (Ser-264 in the mature enzyme). As the phosphopeptide of the T. cruzi PDH E1␣ subunit homolog is highly conserved, these antibodies also detect the T. cruzi phosphorylated protein (expected size: 42.8 kDa). We also used commercial antibodies against the entire mammalian PDH E1␣ subunit, but they did not recognize the TcPDH E1␣ subunit (data not shown), probably because of the low identity (45%) in the amino acid sequence of both homolog proteins. Our results indicate that TcPDH phosphorylation in TcPDP-KO is significantly higher than in parasites transfected with a scrambled sgRNA (Fig. 4, E and F). Interestingly, knockout parasites for the mitochondrial calcium uniporter (TcMCU-KO) we studied previously (38) also exhib-ited an increased level of PDH phosphorylation (Fig. 4, E and F), which suggests a link between mitochondrial Ca 2ϩ uptake, PDP activation and PDH stimulation, as parasites with low mitochondrial Ca 2ϩ levels (TcMCU-KO) exhibit a similar pattern of PDH phosphorylation to TcPDP-KO parasites (Fig. 4, E and F). We also evaluated mitochondrial integrity of TcPDP-KO by measuring citrate synthase activity in these parasites and found it significantly lower than in control cells (Fig. 4G). Then we evaluated oxygen consumption rates (OCR) under ADP-stimulated (state 3), oligomycin-inhibited (state 4), and FCCPstimulated (state 3u) conditions in control (scrambled) and TcPDP-KO digitonin-permeabilized cells, in the presence of succinate or malate and pyruvate as respiratory substrates. Control and PDP-KO mitochondria showed well-coupled respiration, although OCR in the presence of ADP, oligomycin, and FCCP were significantly lower in TcPDP-KO mitochondria (Fig. 4, H-J). This difference was more significant in the presence of malate and pyruvate as mitochondrial substrates. The lower OCR of TcPDP-KO mitochondria correlated with lower citrate synthase activity, suggesting a mitochondrial defect in these cells. We also evaluated the mitochondrial membrane potential (⌬⌿ m ) of knockout and control parasites by measuring Safranine O fluorescence in digitonin-permeabilized epimastigotes in the presence of succinate as the mitochondrial substrate, as described previously (38). When using Safranine O, a decrease in fluorescence after addition of digitonin indicates stacking of the dye to the energized inner mitochondrial membrane (Fig. 4K). Addition of ADP produced the expected small dissipation of membrane potential (observed as a small increase in fluorescence), indicating ADP phosphorylation. ⌬⌿ m returned to its initial level after addition of the adenine nucleotide translocator inhibitor carboxyatractyloside (CAT). Addition of FCCP collapsed the membrane potential. Ablation of TcPDP did not affect the ⌬⌿ m at the steady state or ADP phosphorylation. Under the same conditions, we evaluated the capacity of TcPDP-KO to take up mitochondrial calcium by monitoring the fluorescence of Calcium Green-5N probe in digitonin-permeabilized epimastigotes. In this assay a decrease in fluorescence correlates with a decrease of extramitochondrial Ca 2ϩ , which is concomitant with mitochondrial Ca 2ϩ uptake. The results indicate that the mitochondrial capacity to To investigate the biological relevance of TcPDP we evaluated the growth in vitro of TcPDP-KO and TcPDP-SKO epimastigotes compared with the control (scrambled) cells. We observed that TcPDP-KO parasites exhibited a significantly slower growth rate in rich (LIT) medium than the TcPDP-SKO and the control cells (Fig. 5A), indicating that this protein is required for normal proliferation in vitro. However, when this cell line was grown in low-glucose medium, there was no difference in growth rate compared with control parasites under the same conditions (Fig. 5B). We also investigated the ability of TcPDP-KO epimastigotes to differentiate in vitro into metacyclic trypomastigotes. Metacyclogenesis was significantly impaired in these parasites (Fig. 5C), as well as the ability of trypomastigotes to infect tissue-cultured cells (Fig. 5D). How-ever, the replication of intracellular amastigotes was not affected in TcPDP-KO parasites (Fig. 5E).
Finally, we analyzed the effect of TcPDP down-regulation on cell bioenergetics by measuring the adenine nucleotide levels of knockout and control parasites under normal culture (incubation in LIT medium) and starvation conditions (incubation in PBS), as described previously (38). The results indicate that AMP/ATP ratio is significantly increased under starvation conditions in TcPDP-KO parasites, as compared with control epimastigotes (Fig. 5F). This result is consistent with the increased number of autophagosomes per cell observed in knockout parasites under starvation (PBS), as detected by immunofluorescence microscopy using antibodies against ATG8.1 (Fig. 5G). This is a marker of autophagosomes that has been used previously to evaluate autophagy in T. cruzi (38,45).

The role of pyruvate dehydrogenase phosphatase in T. cruzi
Although TcPDP-KO epimastigotes were found to be bioenergetically affected under starvation conditions, they exhibited similar AMP/ATP ratio than control cells in normal culture medium, suggesting the existence of a compensatory mechanism that overcomes TcPDH inactivation and, as a consequence, the decrease in acetyl-CoA levels, a substrate of the tricarboxylic acid (TCA) cycle. Thus, we investigated whether TcPDP-KO epimastigotes, which showed increased levels of phosphorylated (inactive) PDH, could compensate the decreased synthesis of acetyl-CoA by up-regulating the oxidative metabolism of amino acids, with the production of ammonia that is excreted to the extracellular medium (46). Hence, we monitored ammonia concentration in the culture media of knockout and control parasites at days 2 and 4 of growth in LIT medium. The results indicate that ammonia concentration in the culture medium of TcPDP-KO epimastigotes was significantly higher than that of control cells at day 2 of growth, and this relative increase was much higher after 4 days of cell culture (Fig. 5H), suggesting that a compensatory mechanism involving oxidative metabolism of amino acids is taking place in this mutant cell line.
Taken together our results indicate that TcPDP is a mitochondrial phosphatase sensitive to physiological Ca 2ϩ concentrations in vitro, which could be also Ca 2ϩ stimulated in vivo to specifically activate mitochondrial pyruvate dehydrogenase. Moreover, TcPDP dephosphorylates TcPDH E1␣ subunit in vivo and is required for normal growth, differentiation, infectivity, and energy metabolism in T. cruzi.

Discussion
Our studies have shown that the pyruvate dehydrogenase phosphatase of T. cruzi (TcPDP) and T. brucei (TbPDP) are stimulated by low Ca 2ϩ concentrations, as occurs with vertebrate PDPs, supporting the link between the evolutionary acquisition of a mitochondrial Ca 2ϩ uniporter (MCU) and the presence of Ca 2ϩ -activated mitochondrial dehydrogenases. TcPDP localizes to the mitochondria and is important for normal growth of epimastigotes in rich medium, for differentiation to metacyclic trypomastigotes, and for trypomastigote invasion of host cells in vitro. TcPDP-KO epimastigotes showed increased levels of phosphorylated PDH E1␣ subunit indicating its requirement for in vivo dephosphorylation of this subunit. As compared with control epimastigotes, TcPDP-KO cells have a lower oxygen consumption rate and citrate synthase activity, but higher AMP/ATP ratios and autophagosome formation under starvation conditions. These cells have a compensatory increase in the oxidative metabolism of amino acids to overcome the limited production of acetyl-CoA.
Although the presence of a Ca 2ϩ -regulated PDH complex has been reported in animals, which, together with fungi, belong to the eukaryotic supergroup Ophistokonta, our work demonstrates its presence in trypanosomes, which belong to the supergroup Excavata, one of the earliest branches of eukaryotic evolution. These results reveal an early origin of this Ca 2ϩ signaling pathway and the possibility of its occurrence in the last eukaryotic common ancestor (LECA).
Early binding studies using purified mammalian PDP1c have shown that it possesses an intrinsic Ca 2ϩ -binding site, and a second Ca 2ϩ -binding site that is produced in the presence of PDH E2 subunit (47). Later studies failed to detect binding of Ca 2ϩ to PDP1c alone (48). The T. cruzi lipoylated PDH E2 subunit has been recently identified by Western blot analysis with a polyclonal antibody that detects lipoyl moieties (49), but the gene encoding this protein has not been identified yet, and we could not investigate whether association of TcPDP with TcPDH E2 increased the sensitivity of the enzyme to Ca 2ϩ . However, in agreement with the presence of an intrinsic Ca 2ϩbinding site (47), our results indicate that Ca 2ϩ can activate TcPDP alone although with only a 2-fold increase in activity. This level of activation is lower than that observed for the PDP isolated from different mammalian tissues and assayed in the presence of EGTA-Ca 2ϩ buffers, where at least 5-fold phosphatase activation by Ca 2ϩ has been reported with a K 0.5 value of about 1 M (50 -52). Interestingly, we did not observe a saturable Ca 2ϩ activation with either TcPDP or TbPDP recombinant proteins, as enzyme activation was not observed at concentrations higher than 500 nM and 1 M for TcPDP and TbPDP, respectively. Free Ca 2ϩ concentrations found to stimulate recombinant TcPDP and TbPDP (100 -500 nM and 1 M, respectively) are higher than the cytosolic free Ca 2ϩ concentrations ([Ca 2ϩ ] cyt ) reported for T. cruzi (10 -20 nM in amastigotes and trypomastigotes, 40 -45 nM in epimastigotes (53,54)) and T. brucei (90 -100 nM in procyclic forms, 20 -30 nM in bloodstream forms (54, 55)), respectively. Mitochondrial basal Ca 2ϩ concentrations ([Ca 2ϩ ] mit ) are usually in the same range as [Ca 2ϩ ] cyt . Therefore, in T. cruzi a mitochondrial Ca 2ϩ concentration of 100 nM corresponds to a 2-to 10-fold increase of basal Ca 2ϩ concentration, whereas in T. brucei a free Ca 2ϩ concentration of 1 M corresponds to a 10-to 50-fold increase in Ca 2ϩ concentration, which appears to be very high. It thus looks as if Ca 2ϩ regulation is more likely to occur in T. cruzi than in T. brucei. Experiments using the photoprotein aequorin targeted to the mitochondria and cytosol of T. brucei procyclic forms and treated with melittin (a plasma membranepermeabilizing agent) or the acidocalcisome Ca 2ϩ -releasing agent monensin found increases in [Ca 2ϩ ] mit up to 7-to 12-fold higher than [Ca 2ϩ ] cyt reaching micromolar values (56). These increases are compatible with the free Ca 2ϩ concentration observed to stimulate recombinant TbPDP and a role of the enzyme in these stages.
Using the CRISPR/Cas9 system we developed for T. cruzi (42), we generated a knockout cell line for TcPDP. We also used an enhanced Cas9 (eSpCas9) (44) to reduce the possibility of off-target effects. However, it was not possible to generate a double knockout using the eSpCas9 nuclease. After three attempts, only single knockout cell lines were detected by PCR analysis of clonal populations. Sequence analysis of both alleles did not show nucleotide differences at the chosen protospacer for sgRNA targeting. It seems that eSpCas9 is less efficient than regular Cas9 in T. cruzi, or at least it was for this specific locus. However, a double knockout homogeneous population for TcPDP was obtained using the conventional Cas9, with a specific sgRNA that is predicted not to generate off-target effects in the T. cruzi genome. Phenotypic evaluation of this mutant cell line indicates that TcPDP is required for TcPDH dephosphorylation. Interestingly, a TcMCU-KO cell line we generated in a The role of pyruvate dehydrogenase phosphatase in T. cruzi previous study (38) also exhibited a significant higher level of PDH E1␣ subunit phosphorylation, although lower than that of TcPDP-KO parasites. An explanation for this difference is that basal Ca 2ϩ could slightly activate TcPDP in TcMCU-KO cell line, thus resulting in some PDH dephosphorylation that does not occur in TcPDP-KO parasites. The reduced citrate synthase activity observed in these mutant epimastigotes evidences a down-regulation of the TCA cycle, which could be the consequence of higher levels of inactive (phosphorylated) TcPDH. A direct consequence of a defective TCA cycle is the decrease in the oxygen consumption rate observed in TcPDP-KO cells. Our results show that TcPDP-KO-permeabilized epimastigotes exhibit a lower oxygen consumption rate than control cells when pyruvate (the PDH substrate) and malate are used as mitochondrial substrates in the respiration medium. However, respiration was less impaired in TcPDP-KO cells when succinate was used as mitochondrial substrate, probably because in this way the need of acetyl-CoA from pyruvate is being bypassed. A similar result was observed for Saccharomyces cerevisiae ⌬ptc6 mutant, which lacks the gene encoding PDP (57). This strain exhibited lower OCR than WT cells when grown on pyruvate-based media.
The impaired respiration observed in TcPDP knockout epimastigotes correlates with the growth deficiency observed in these cells. This phenomenon was observed previously in epimastigotes lacking the MCUb subunit of the T. cruzi MCU complex (38), indicating that this protein is important for normal proliferation in vitro. However, no difference in growth rate compared with control cells was observed when TcPDP-KO was grown in low glucose medium, probably because under these conditions a slower glycolysis rate provides less pyruvate as PDH substrate. So, growth differences resulting from PDH inactivation in TcPDP-KO cells (LIT medium) are not observed in low-glucose medium and parasites grow as control cells. Moreover, epimastigotes have a large pool of free amino acids that is used to maintain cell osmolarity (58). Increased amino acid oxidation through the TCA cycle would counteract the lack of PDH-catalyzed formation of acetyl-CoA from pyruvate. In this regard, the increased amount of ammonia detected in the culture medium of TcPDP-KO parasites after 2-4 days, compared with control epimastigotes, evidences an increase in amino acid catabolism to compensate the acetyl-CoA deficiency in this mutant cell line.
Ca 2ϩ signaling is required for T. cruzi epimastigote differentiation (59) and for host cell invasion by trypomastigotes (60). In agreement with this requirement, a defect in the differentiation of epimastigotes into infective metacyclic trypomastigotes, and also in the infection of Vero cells by trypomastigotes, was observed in TcPDP-KO parasites, probably because Ca 2ϩ stimulates TcPDP and consequently activates PDH and energy metabolism. However, replication of intracellular amastigotes was unaffected. Amastigotes face a low-glucose condition in the host cell cytosol (29), and pyruvate is equally scarce in either TcPDP-KO or control parasites; therefore, no differences in intracellular replication were observed. The predominance of fatty acid oxidation over glycolysis in the intracellular stages, as suggested by the increased levels of fatty acid oxidation enzymes (61), would also explain that TcPDP is not required for the replication of amastigotes. Moreover, amino acid catabolism has been shown to be a main energy source in intracellular T. cruzi stages (61,62). Amino acid consumption leads to the production of ammonia (NH 3 ), which needs to be excreted to avoid toxicity. In this regard, two proteins involved in NH 3 detoxification have been recently shown to be required for normal proliferation of T. cruzi amastigotes, the ammonium transporter (TcAMT) (63) and the glutamine synthetase (64). Thus, their combined activity could contribute to the normal replication phenotype observed in TcPDP-KO amastigotes. Nevertheless, changes in the calcium regulation of TcPDP cannot be ruled out in different T. cruzi stages. In general, the biological defects observed in TcPDP knockout parasites highlight the importance of phosphorylation-mediated TcPDH regulation for parasite survival at different stages of the T. cruzi life cycle.
An increase in AMP/ATP ratio was observed in TcPDP-KO epimastigotes, indicating a bioenergetic imbalance that was stronger under starvation. Concomitantly, autophagy was also increased in knockout parasites and this effect was more evident under nutrient deprivation. In mammalian cells, downregulation of MCU promotes autophagy (65). This has also been reported in T. brucei (26) and T. cruzi (38). A defective TCA cycle produced by PDP down-regulation could have a similar effect. The increase in the AMP/ATP ratio produced by down-regulation of the TCA cycle could promote autophagy by stimulation of AMP-dependent kinase (AMPK) (65). However, this correlation was not previously observed in T. cruzi when characterizing MCU and MCUb subunits of the MCU complex (38), suggesting an AMPK-independent autophagy pathway similar to that of T. brucei (66). Further studies should be performed to elucidate the mechanism of autophagy induction in T. cruzi.
In summary, we have identified a mitochondrial pyruvate dehydrogenase phosphatase in T. cruzi that is sensitive to physiological Ca 2ϩ concentrations, suggesting that it could also be Ca 2ϩ stimulated in vivo to specifically activate mitochondrial PDH. Moreover, TcPDP dephosphorylates TcPDH, stimulates energy metabolism through TCA cycle activation, and is required for parasite normal development at different stages of its life cycle. These findings provide new insights into the role of Ca 2ϩ signaling in T. cruzi cell bioenergetics.

Cell culture
T. cruzi Y strain epimastigotes were cultured in liver infusion tryptose (LIT) medium containing 10% heat-inactivated fetal bovine serum (FBS) at 28°C (68). CRISPR/Cas9 mutant cell lines were maintained in medium containing 250 g/ml G418 and 10 g/ml blasticidin or 5 g/ml puromycin. Epimastigotes overexpressing TcPDP-3xHA were cultured in medium containing 250 g/ml G418. The growth rate of epimastigotes was determined by counting cells in a Muse® Cell Analyzer. Tissue culture cell-derived trypomastigotes were obtained from Vero cells infected with metacyclic trypomastigotes obtained as described below. T. cruzi trypomastigotes were collected from the culture medium of infected host cells, using a modification of the method of Schmatz and Murray (69) as described previously (60). Vero cells were grown in RPMI supplemented with 10% fetal bovine serum and maintained at 37°C with 5% CO 2 .

TcPDP overexpression
TcPDP ORF (1248 nt) was PCR amplified (primers 1 and 2, Table 1) and cloned into the pTREX-n/3xHA vector (38) by restriction sites XbaI/XhoI and subsequently used to transfect T. cruzi epimastigotes. Gene cloning was confirmed by PCR and sequencing. TcPDP overexpression was confirmed by Western blot analysis using anti-HA antibodies.

TcPDP endogenous C-terminal tagging
To achieve the C-terminal tagging of endogenous TcPDP we used the method we developed for T. cruzi (39,40) to clone a specific sgRNA sequence targeting the 3Ј end of TcPDP gene (TriTrypDB ID: TcCLB.506739.200) into Cas9/pTREX-n vector (42). The construct (3Јend-sgRNA/Cas9/pTREX-n), together with a DNA donor cassette to induce homology-directed repair, was used to co-transfect T. cruzi epimastigotes and to insert a specific 3xc-Myc tag sequence at the 3Ј end of the gene. The sgRNA targeting TcPDP 3Ј end was obtained by PCR from plasmid pUC_sgRNA as described previously (42) using specific oligonucleotides (primers 3 and 4, Table 1).
To avoid Cas9 off-targeting, protospacer was analyzed with EuPaGDT Design Tool. A DNA donor cassette containing the 3xc-Myc tag sequence and the puromycin resistance gene was amplified using the pMOTag23M vector (67). Template for homologous-directed repair was amplified by PCR with 120 bp ultramers, of which 100 bp correspond to regions located right upstream of the stop codon and downstream of the Cas9 target site of the TcPDP gene, and 20 bp for annealing on the pMOTag23M vector (primers 5 and 6, Table 1). PCR reaction was carried out using the following cycling conditions: initial denaturation for 2 min at 95°C; followed by 40 cycles of 20 s at 95°C, 20 s at 63°C, and 1 min 40 s at 72°C; then a final extension for 10 min at 72°C. Epimastigotes co-transfected with Rv_TcPDP-KO_check CATGGGTGAGTGAATGTTTCTG 20 Fw_TcPDP_probe ATGCCGAGCAGCAGCAAAAAG 21 Rv_TcPDP_probe GAAGAAGATCCTGGAACTGC

The role of pyruvate dehydrogenase phosphatase in T. cruzi
3Јend-sgRNA/Cas9/pTREX-n and DNA donor were cultured for 5 weeks with G418 and puromycin for selection of doubleresistant parasites. Endogenous gene tagging was verified by PCR from gDNA using a specific primer set (primers 7 and 8, Table 1) and by Western blot analysis.

Gene cloning and protein heterologous expression
TcPDP and TbPDP open reading frames (gene IDs: TcCLB.506739.200 and Tb427.05.1660) were PCR-amplified from T. cruzi Y strain gDNA and T. brucei Lister 427 gDNA, respectively, using specific primer sets (primers 9 -12, Table 1), and separately cloned into vector pET32 Ek/LIC (Novagen) for heterologous expression in bacteria. The sequence of recombinant clones was verified, and then they were independently transformed by heat shock into E. coli BL21 Codon Plus (DE3)-RIPL chemically competent cells. Expression of recombinant TcPDP and TbPDP proteins was induced with 0.5 mM isopropyl-␤-D-thiogalactopyranoside (IPTG) in LB broth for 3 h at 37°C. Total lysates from induced and uninduced recombinant bacteria were analyzed by SDS-PAGE to confirm the expression of both enzymes.

Purification of recombinant PDPs under native conditions
Cell pellet from 500-ml cultures of recombinant E. coli BL21 Codon Plus (DE3)-RIPL cells expressing TcPDP or TbPDP were resuspended and incubated for 30 min on ice in 40 ml of cold lysis buffer: 50 mM Tris-HCl, pH 7.6, 150 mM sodium chloride, 10 mM imidazole, 0.1% Triton X-100, 0.1 mg/ml lysozyme, 25 units/ml Benzonase nuclease, and protease inhibitor mixture for purification of histidine-tagged proteins (Sigma P8849; 50 l/g of cell paste). Then three sonication pulses (40% amplitude, 30 s, on ice) were applied to ensure the complete disruption of cells. After centrifugation at 20,000 ϫ g for 30 min at 4°C, supernatant was clarified by passing it through a 0.2 m pore nitrocellulose filter. Protein extract was kept on ice and used for immediate purification of recombinant TcPDP or TbPDP. Protein purification was performed at 4°C using His-Pur Ni-NTA Chromatography Cartridges, following the manufacturer's protocol for histidine-tagged protein purification under native conditions. Tris-buffered saline (TBS: 50 mM Tris-HCl, pH 7.6, 150 mM sodium chloride) replaced PBS in all the steps during the purification process to avoid phosphate contamination in the PDP enzymatic reaction. One-ml fractions were eluted (elution buffer: 250 mM imidazole in Tris-buffered saline), and buffer exchange was performed immediately using PD-10 desalting columns to finally obtain the protein in 50 mM HEPES, pH 7.0. All purification steps were verified by SDS-PAGE. Desalted recombinant proteins were quantified using the Pierce BCA Protein Assay Kit.

PDP activity assay
TcPDP activity was assayed by quantifying phosphate release from a synthetic phosphopeptide corresponding to a 14 amino acid long segment of TcPDH E1␣ subunit surrounding phosphorylation sites (Ser) 1 and 2: Tyr-Val-Gly-His-Ser-Met-Ser-Asp-Pro-Asp-Ser-Gln-Tyr-Arg (Fig. 1), as described previously for the mammalian enzyme (41, 70, 71). Enzymatic reactions were performed for 1 h at room temperature in a 100-l reac-tion containing 200 M substrate peptide, 50 mM Hepes, pH 7.0, 50 M MgCl 2 , 2 mM DTT, and 120 g of BSA. Different Ca 2ϩ concentrations (from 10 Ϫ9 to 10 Ϫ4 M) were achieved using a CaCl 2 -EGTA buffer and calculated with the freely distributed software MaXChelator based on the Bers stability constants, as described previously (72). The reaction was stopped by adding freshly mixed malachite green reagent (3 parts 0.045% malachite green and 1 part 4.2% ammonium molybdate, 4 M HCl), and absorbance at 660 nm was immediately read on a microplate spectrophotomer (Molecular Devices). A potassium phosphate standard curve was included in the assay for quantification purposes. TbPDP activity was assayed following the same methodology, but using a homolog synthetic phosphopeptide corresponding to a 14 amino acid long segment of TbPDH E1␣ subunit surrounding phosphorylation sites (Ser) 1 and 2: Tyr-Met-Gly-His-Ser-Met-Ser-Asp-Pro-Asp-Ser-Gln-Tyr-Arg, as TbPDP substrate (Fig. 1).

TcPDP-KO
A single guide RNA sequence to target the gene coding for the hypothetical TcPDP protein was PCR-amplified from plasmid pUC_sgRNA, as previously described (42). Selection of protospacer was performed using EuPaGDT (Eukaryotic Pathogen CRISPR guide RNA Design Tool, http://grna.ctegd. uga.edu/) 6 (80). The protospacer sequence was included into the forward primer while using a common reverse primer for sgRNA amplification. These primers also contained a BamHI restriction site for cloning into Cas9/pTREX-n (42) to generate TcPDP-sgRNA/Cas9/pTREX-n construct. The sgRNA orientation was verified by PCR using the specific TcPDP-sgRNA forward primer and the HX1 reverse primer (42). Positive clones that generate a 190-bp PCR fragment were also sequenced. A scrambled sgRNA (Scr-sgRNA/Cas9/pTREX-n) was used as control. A DNA donor cassette designed to promote homologous directed repair and replacement of TcPDP ORF was obtained by PCR using a set of long primers (ultramers) containing 120 nucleotides, from which 100 nucleotides correspond to the first 100 nt (forward ultramer) and the last 100 nt (reverse ultramer) of TcPDP ORF, and 20 nt annealing on Bsd gene. Circular construct TcPDP-sgRNA/Cas9/pTREX-n and linear Bsd cassette were used to co-transfect T. cruzi epimastigotes. After 5 weeks of selection with 250 g/ml G418 and 10 g/ml blasticidin, TcPDP gene replacement was verified by PCR. Primers used to generate TcPDP-KO are listed in Table 1.

Southern blot analysis
To confirm TcPDP gene knockout 25 g of gDNA from control (transfected with scramble sgRNA) and TcPDP-KO epimastigotes were digested with PvuII enzyme and resolved on a 0.8% agarose gel. Restriction fragments were transferred to nylon membrane and hybridized with a biotin-labeled probe corresponding to the first 340 nt of TcPDP ORF that was amplified by PCR using primers 20 and 21 (Table 1) and labeled using the North2South TM Biotin Random Prime Labeling Kit. Hybridization, posthybridization washes, and detection were performed with North2South TM Chemiluminescent Hybridization and Detection Kit, following manufacturer's recommendations. Signal detection was performed using UVItec Alliance Gel Documentation System (UVItec, Cambridge, UK).

Immunofluorescence analysis
Epimastigotes were incubated with 100 nM MitoTracker® Deep Red FM for 30 min at 28°C in culture medium. Then cells were washed with PBS and fixed with 4% paraformaldehyde in PBS for 1 h at RT. Cells were allowed to adhere to poly-L-lysinecoated coverslips and then permeabilized for 5 min with 0.1% Triton X-100. Permeabilized cells were blocked with PBS containing 3% BSA, 1% fish gelatin, 50 mM NH 4 Cl, and 5% goat serum overnight at 4°C. Then, cells were incubated with a primary antibody (monoclonal anti-HA-Tag (1:500) or monoclonal anti-c-Myc-tag (1:10)), diluted in 1% BSA in PBS (pH 8.0) for 1 h at RT. Cells were washed three times with 1% BSA in PBS (pH 8.0), and then incubated for 1 h at RT in the dark with Alexa Fluor 488 -conjugated goat anti-mouse secondary antibody (1:1000). Then, cells were washed and mounted on slides using Fluoromount-G mounting medium containing 5 g/ml of 4Ј,6diamidino-2-phenylindole (DAPI) to stain DNA. Controls were performed as described above but in the absence of a primary antibody. Differential interference contrast and fluorescence optical images were captured with a confocal microscope Leica TCS SP5 II, with a 100ϫ objective (1.44 aperture) under nonsaturating conditions, that uses photomultiplier tubes for detection of emission, and LAS AF software (Leica, Wetzlar, Germany) for acquisition and processing of digital images.

Metacyclogenesis
We followed the protocol described by Bourguignon et al. (75) with minor modifications. Epimastigotes were obtained after 4 days in LIT medium and incubated for 2 h in triatome artificial urine (TAU) medium (190 mM NaCl, 17 mM KCl, 2 mM MgCl 2 , 2 mM CaCl 2 , 0.035% sodium bicarbonate, 8 mM phosphate, pH 6.9) at room temperature). Then, parasites were incubated for 96 h in TAU 3AAG medium (TAU medium supplemented with 10 mM L-proline, 50 mM sodium L-glutamate, 2 mM sodium L-aspartate, and 10 mM glucose). To increase the number of metacyclic forms to infect Vero cells, the contents of the flask were collected and resuspended in medium containing fresh fetal bovine serum and incubated at 37°C for 20 h. The complement in fresh FBS kills epimastigotes whereas metacyclic trypomastigotes survive. Samples were harvested from the TAU 3AAG plus FBS-containing medium at days 5 and 10 of cultivation.

In vitro infection assay
Gamma-irradiated (2000 radiation-absorbed doses) Vero cells (4.5 ϫ 10 5 cells) were plated onto sterile coverslips in a 12-well plate and incubated overnight at 35°C, 7% CO 2 , in RPMI medium plus 10% fresh fetal bovine serum. Tissue culture-derived trypomastigote collections were incubated at 4°C overnight to allow amastigotes to settle from swimming trypomastigotes. Trypomastigotes from the supernatants of these collections were counted and used to infect the coverslips at a 10:1 ratio of parasites to host cells. At 4 h post infection, coverslips were washed extensively with Hanks' balanced salt solution, followed by PBS, pH 7.4, to remove any extracellular parasites. Coverslips were fixed immediately in 4% paraformaldehyde in PBS, pH 7.4, at 4°C for 30 min. Coverslips were washed once with PBS and mounted onto glass slides in Fluoromount-G containing 15 g/ml DAPI, which stains host and parasite DNA. Coverslips were viewed on an Olympus BX60 microscope to quantify the number of host cells that contained intracellular parasites and the number of intracellular parasites The role of pyruvate dehydrogenase phosphatase in T. cruzi per cell in randomly selected fields. Three hundred host cells were counted per sample in three independent experiments. To quantify amastigote replication, the following modifications were used: host cells were infected at a ratio of 10 parasites to 1 host cell, and coverslips were allowed to incubate for 48 h post infection at 35°C, 7% CO 2 , prior to fixation and DAPI staining.

Cellular respiration
The OCR of digitonin-permeabilized epimastigotes was measured using a high-resolution respirometer (Oroboros Oxygraph-2k, Innsbruck, Austria) with DatLab 4 software for data acquisition and analysis, and calibrated as reported by their manufacturers. Cells (1 ϫ 10 8 ) were incubated at 28°C in a 2 ml chamber containing 125 mM sucrose, 65 mM KCl, 10 mM Hepes-KOH, pH 7.2, 2.5 mM K 2 PO 4 , 1 mM MgCl 2 , 50 M EGTA, 5 mM succinate, and 25 M digitonin. Alternatively, 5 mM malate and 5 mM pyruvate were used as mitochondrial substrates, instead of succinate. OCR was calculated as the negative time derivative of oxygen concentration measured in the closed respirometer chambers and expressed per milligram of protein.
Data were recorded at 2 s intervals, and 10 data points were used to calculate the slope of the OCR plot through a polynomial fit with DatLab 4 software, as described (76).

Citrate synthase activity
Citrate synthase activity was measured using a previously described protocol (77) adapted to trypanosomes (38). Briefly, the conversion of oxaloacetate and acetyl-CoA to citrate and SH-CoA was monitored by quantification of the colorimetric product thionitrobenzoic acid (78). T. cruzi epimastigotes in early exponential phase (ϳ1 ϫ 10 8 cells) were washed twice with PBS and incubated in lysis buffer (10 mM Tris-HCl, pH 7.4, 1 mM EDTA, 0.1% Triton X-100, and 25 units of Benzonase nuclease) for 10 min on ice. Then, proteins were quantified by BCA protein assay and 260 l reactions were set up in buffer containing 5 g protein, 250 M oxaloacetate, 50 M acetyl-CoA, 100 M 5,5Ј-dithio-bis(2-nitrobenzoic acid), and 10 mM Tris-HCl, pH 8.0. The increase in absorbance at 412 nm was monitored for 20 min at 28°C using a microplate reader (PowerWave XS 2, BioTek Instruments, Winooski, VT). Values of V max were normalized taking scrambled cell line as reference value.

Mitochondrial calcium uptake
The uptake of Ca 2ϩ by permeabilized T. cruzi epimastigotes was assayed by fluorescence of Calcium Green-5N probe at 28°C, as described previously (38). Briefly, cells were collected by centrifugation at 1000 ϫ g for 7 min and washed twice with Buffer A with glucose (116 mM NaCl, 5.4 mM KCl, 0.8 mM MgSO 4 , 5.5 mM D-glucose, and 50 mM HEPES, pH 7.0). Epimastigotes were resuspended to a final density of 1 ϫ 10 9 cells/ml in Buffer A with glucose and kept on ice. Before each experiment, a 50-l aliquot of T. cruzi epimastigotes (5 ϫ 10 7 cells) was added to the reaction buffer (125 mM sucrose, 65 mM KCl, 10 mM Hepes-KOH buffer, pH 7.2, 1 mM MgCl 2 , 2.5 mM potassium phosphate; 1.95 ml) containing 5 mM succinate, 20 M free Ca 2ϩ , and 0.5 M Calcium Green-5N. Mitochondrial Ca 2ϩ uptake was initiated by the addition of 50 M digitonin. Fluorescence changes were monitored in a F-4500 Fluorescence Spectrophotometer (Hitachi) with excitation at 506 nm and emission at 532 nm.

Autophagy assay
Expression of the TcAtg8.1 autophagy marker and autophagosome formation in T. cruzi epimastigotes grown in LIT medium and under starvation conditions was estimated by immunofluorescence analyses using anti-TcATG8.1 antibody as described (45). For starvation induction, mid-log parasites were washed twice with PBS, resuspended in the same buffer at a concentration of 5 ϫ 10 7 cells/ml, and incubated for 16 h at 28°C as described previously (45).

Adenine nucleotide levels
Control (transfected with scrambled sgRNA) and TcPDP-KO epimastigotes were harvested and washed once with Buffer A (116 mM NaCl, 5.4 mM KCl, 0.8 mM MgSO 4 and 50 mM HEPES at pH 7.0). After washing, 1 ϫ 10 8 cells per treatment were centrifuged and resuspended in 100 l of Buffer A, and then lysed on ice for 30 min by addition of 150 l of 0.5 M HClO 4 . The lysates were neutralized (pH 6.5) by addition of 60 l of 0.72 M KOH/0.6 M KHCO 3 . Samples were centrifuged at 1000 ϫ g for 5 min and the supernatant was separated for adenine nucleotide determination. ATP, ADP, and AMP in extracted samples were quantified by a luciferin-luciferase bioluminescence assay in a plate reader as described (38). Briefly, we used an ATP Determination Kit according to manufacturer's instructions, with adenylate kinase and/or nucleosidediphosphate kinase (NDK) (Sigma). To determine the amount of adenine nucleotides, four measurements were taken of three different reactions for each sample by end point determination of the ATP concentration: One reaction without addition of any ATP-generating enzyme (for ATP), another reaction adding NDK (for ATP ϩ ADP), and the third reaction adding both adenylate kinase and NDK (for ATP ϩ ADP ϩ AMP). The amount of ADP was obtained by subtracting the ATP value from the (ATP ϩ ADP) value and the amount of AMP was calculated from the difference between the (ATP ϩ ADP ϩ AMP) content and the (ATP ϩ ADP) content.

The role of pyruvate dehydrogenase phosphatase in T. cruzi Ammonia determination in culture medium
Cell cultures of T. cruzi epimastigotes were started at 2 ϫ 10 6 cells/ml (day 0). Ammonia concentration in culture medium was determined using an Ammonia Assay Kit (Sigma, catalogue no. AA0100) at days 0, 2, and 4 following manufacturer's instructions. The assay was adapted to be performed in 96-well plates. Briefly, 1 ml of cell culture was centrifuged at 1000 ϫ g for 5 min and supernatant was transferred to a new tube. Then, supernatants were diluted 1:10 in ultrapure water and filtered through 0.2 m pore nitrocellulose membrane. Then 20 l from each filtered sample were distributed in triplicate into 96-well plates, including water as reagent blank and ammonia standard solution as assay control. Two hundred microliters of Ammonia Assay Reagent were added to each well and samples were incubated 5 min at RT. Absorbance at 340 nm was immediately measured in a plate reader (Molecular Devices). Subsequently, 2 l of L-Glutamate Dehydrogenase solution (Sigma, catalogue no. G2294) were added to each well, and after 5 min incubation at RT absorbance at 340 nm was measured again. Ammonia concentration of samples was calculated according to the kit's protocol.

Statistical analysis
Statistical analyses were performed with GraphPad Prism software (La Jolla, CA), version 6.0. Reported values are mean Ϯ S.D. of n biological experiments, as indicated in the figure legends. The level of significance was evaluated by Student's t test for comparisons between two cell lines, one-way analysis of variance (ANOVA) for comparisons between more than two cell lines, and two-way ANOVA with multiple comparison tests for analyses of grouped data.