The ER-localized Ca2+-binding protein calreticulin couples ER stress to autophagy by associating with microtubule-associated protein 1A/1B light chain 3

Autophagy is of key importance for eliminating aggregated proteins during the maintenance of cellular proteostasis in response to endoplasmic reticulum (ER) stress. However, the upstream signaling that mediates autophagy activation in response to ER stress is incompletely understood. In this study, in vivo and in vitro approaches were utilized that include gain- and loss-of-function assays and mouse livers and human cell lines with tunicamycin-induced pharmacological ER stress. We report that calreticulin, a quality control chaperone that binds to misfolded glycoproteins for refolding in the ER, is induced under ER stress. Calreticulin overexpression stimulated the formation of autophagosomes and increased autophagic flux. Interestingly, calreticulin was sufficient for attenuating ER stress in tunicamycin- or thapsigargin-treated HeLa cells, whereas lentivirus-mediated shRNA calreticulin knockdown exacerbated ER stress. Mechanistically, we noted that calreticulin induces autophagy by interacting with microtubule-associated protein 1A/1B-light chain 3 (LC3). Confocal microscopy revealed that the colocalization of calreticulin and LC3 at the autophagosome was enhanced under ER stress conditions. Importantly, a conserved LC3-interacting region was necessary for calreticulin-mediated stimulation of autophagy and for reducing ER stress. These findings indicate a calreticulin-based mechanism that couples ER stress to autophagy activation, which, in turn, attenuates cellular stress, likely by alleviating the formation of aberrantly folded proteins. Pharmacological or genetic approaches that activate calreticulin–autophagy signaling may have potential for managing ER stress and related cellular disorders.

There are three well-characterized ER stress sensors of the UPR: dsRNA-activated protein kinase (PKR)-like ER kinase (PERK), activating transcription factor 6 (ATF6), and inositolrequiring enzyme 1 (IRE1) (2,3). Homeostasis of the ER is necessary for the maintenance of protein homeostasis, also called proteostasis. Overloaded unfolded or misfolded proteins are detained in the ER and cause ER stress (4,5). To deal with the proteostasis abrogation caused by ER stress, the UPR is triggered to reduce the protein overload (4 -7). However, sustained ER stress leads to various metabolic disorders associated with obesity, insulin resistance, and type 2 diabetes (8).
Upon ER stress, the UPR plays important roles in protein folding and degradation, whereas deregulation of the UPR causes metabolic disorders (2,5,9,10). Specially, the ER-associated degradation (ERAD) pathway is activated by the UPR to remove unfolded or misfolded proteins to ameliorate ER stress. Besides the canonical ubiquitin-proteasome system, autophagy, a highly conserved protein degradation pathway from yeast to mammals, is considered a noncanonical ERAD pathway (11). Autophagy is a quality control system that degrades cellular content in the lysosome, in which the cytoplasmic materials are engulfed in double-membraned vesicles called autophagosomes (12,13). The autophagosome elongation step involves two ubiquitin-like reaction processes, including formation of the Atg12-Atg5-Atg16L1 complex and conjugation of phosphatidylethanolamine to LC3 by sequential action of Atg4, Atg7, and Atg3 (14). It has been reported that genetic overexpression or the transgene of Atg5 or LC3 robustly augments autophagic activity and accelerates the elimination of substrates (15)(16)(17)(18)(19). On the contrary, knockdown or loss-of-function mutation of these two molecules causes autophagic degradation deficiency and substrate accumulation (20 -22).
Autophagy is triggered upon ER stress to eliminate excess proteins and protect cells against metabolic damage (23)(24)(25). It has been reported that the activation of PERK and IRE1 and increased [Ca 2ϩ ] cyt /[Ca 2ϩ ] ER mediates ER stress-induced autophagy (23, 26 -28). However, the signaling pathways that couple ER stress to autophagy are not completely understood.
Calreticulin, an ER-localized Ca 2ϩ -binding chaperone, is known for its roles in protein folding and quality control. Cal-reticulin, together with its homolog calnexin, monitors and controls the quality of newly produced glycoproteins via the "calreticulin/calnexin cycle" (29 -31). In this circular supervision system, the folding levels of substrate proteins are checked to ensure that only correctly folded proteins can exit from the folding cycle within the ER membrane to the Golgi (31). However, whether and how calreticulin alleviates ER stress via other mechanisms is not understood.
Recently, we demonstrated that activation of the UPR and autophagy attenuates hepatic steatosis and insulin resistance (2,32,33). Here we sought to investigate the mechanisms of ER stress-induced activation of autophagy and its role in alleviating ER stress. These data suggest that ER stress induces expression of calreticulin, calreticulin stimulates the formation of autophagosome and induces autophagic flux, activation of calreticulin is sufficient to attenuate ER stress, and the LIR motif of calreticulin is of key importance in connecting autophagic machinery to inhibit ER stress. These findings provide a new insight into the molecular mechanisms of ER stress rescue signaling and may potentially become a novel therapeutic target for stress-induced disorders and proteostasis deficiencies.

ER stress stimulates expression of calreticulin
Although it has been well-known that the expression of various chaperones is responsively stimulated to cope with ER stress conditions, evidence of calreticulin stimulation is scarce (34). To demonstrate whether calreticulin is stimulated by ER stress, we generated an ER stress murine model via intraperitoneal administration of an ER stress inducer, tunicamycin, and determined the hepatic expression of calreticulin. As expected, the livers of tunicamycin-treated mice showed significantly increased levels of GRP78, a representative marker of ER stress, compared with control individuals (Fig. 1A). The protein expression of hepatic calreticulin was increased in tunicamycin-treated mice (Fig. 1, A and B). Consistent with these findings, as shown in Fig. 1, C-F, calreticulin was dose-dependently up-regulated in HeLa cells treated with tunicamycin and another ER stress inducer, thapsigargin. Notably, the induction of ER stress by tunicamycin or thapsigargin was evidenced by the induction of GRP78, CHOP, and XBP1. These observations were highly consistent with previous reports (34) and led us to explore the significance of calreticulin up-regulation in response to ER stress conditions.

Calreticulin is necessary and sufficient to relieve ER stress
Given that calreticulin acts as a chaperone to assist protein folding, we hypothesized that calreticulin deficiency may lead to exacerbated ER stress. To confirm this hypothesis, calreticulin-deficient or control HeLa cells were generated using a lentivirus stably expressing calreticulin shRNA (shCRT) or negative control shRNA (shNC). As shown in Fig. 2A, the protein levels of calreticulin were effectively suppressed by shCRT#2 but not by the other shRNA, shCRT#1. Next, cells stably expressing shCRT#2 and shNC were treated with tunicamycin or thapsigargin, and the representative ER stress markers GRP78 and CHOP were measured. Immunoblotting and realtime quantitative PCR were performed to guarantee the efficiency of RNAi in shCRT (Fig. 2, B, C, E, and F). As expected, shCRT cells showed increased protein levels of GRP78 Calreticulin induces autophagy under ER stress ( Fig. 2, B and E) and increased mRNA levels of GRP78 and CHOP (Fig. 2, D and G), which indicated profound sensitivity to drug-induced ER stress.
To further illustrate the function of calreticulin in ER stress, we transiently overexpressed myc-CRT in HeLa cells, treated the cells with tunicamycin or thapsigargin, and then determined the ER stress levels by immunoblotting. In contrast to the results of knockdown experiments, a progressive decrease in GRP78 was observed when the calreticulin expression level was gradually increased (Fig. 3, A and B), suggesting a reduction of ER stress. These data support a protective role of calreticulin in response to cellular stress.

Calreticulin positively regulates autophagic flux under ER stress
We next sought to investigate the underlying mechanism of calreticulin ameliorating ER stress. In previous studies, a set of chaperones were revealed to facilitate the degradation of unfolded or misfolded proteins under stress conditions (4 -7, 35, 36). Based on these discoveries, we presumed that calreticulin may be activated in response to ER stress to function similarly. Thus, we focused our study on the relationship between calreticulin and protein degradation machineries. We found that autophagic levels were elevated under the same condition,

Calreticulin induces autophagy under ER stress
represented by an increase in the LC3-II to LC3-I ratio (Fig. 4, A  and B). Notably, the expression levels of p62 are decreased by CRT under tunicamycin and thapsigargin treatment, which is consistent with the autophagic stimulating effects of CRT. Immunofluorescence imaging manifested more LC3 puncta in the cytoplasm of calreticulin-overexpressing cells, which indicated a growth in autophagosome numbers (Fig. 4, C and D). These results indicate that calreticulin promotes autophagic flux in response to ER stress. Moreover, as shown in Fig. 4, E and F, knockdown of calreticulin decreased the conversion of LC3-I to LC3-II in HeLa cells treated with doses of tunicamycin or thapsigargin, suggesting that calreticulin is necessary for ER stress-induced autophagy. Together, these data indicate that calreticulin may couples ER stress signals to stimulate autophagy.

Calreticulin associates with LC3 through the LIR
To gain deep insight into the link between calreticulin and the autophagic degradation pathway, we studied the interaction between calreticulin and autophagic proteins. Strikingly, an evolutionarily conserved LIR, containing the motif W/Y/ FXXL/I/V (X represents any residue) and existing in most LC3interacting proteins (37), was identified in human calreticulin protein from amino acids 200 to 204 (Fig. 5A). To verify the interaction of calreticulin with LC3, HEK293T cells were trans-

Calreticulin induces autophagy under ER stress
fected with GST-tagged LC3 and GFP-tagged calreticulin constructs, and then GST pulldown was performed. As shown in Fig. 5B, the subsequent immunoblotting results showed a clear band of GFP-calreticulin, in contrast to the GST vector-expressing control group. To identify the binding site of calreticulin, we mutated the key residues Trp 200 and Leu 203 into Ala to establish the null mutation of the LIR motif (W200A, L203A) of calreticulin (calreticulin ⌬LIR) and subjected the mutant to a GST pulldown test. Strikingly, the calreticulin ⌬LIR mutant does not bind GST-tagged LC3 (Fig. 5B). These results demonstrate that the LIR motif is essential for the association between calreticulin and LC3. We supposed that the binding capacity of calreticulin with LC3 might be altered under ER stress conditions. Thus, we measured the amount of coimmunoprecipitated calreticulin under treatment with ER stress inducers. To avoid possible influence of alterations of endogenous protein expression under stress conditions, GFP-tagged calreticulin and LC3 were overexpressed in HEK293T cells, and then GST pulldown was performed. Interestingly, the association between calreticulin and LC3 was profoundly increased in a dose response to tunicamycin or thapsigargin treatment (Fig. 5, C and D). In addition, an immunofluorescence imaging analysis was performed. As shown in Fig. 5, E and F, compared with the control vehicle, treatment with tunicamycin increased the colocalization of calreticulin and LC3 under chloroquine conditions in HeLa cells. Notably, increased numbers of LC3 puncta by tunicamycin were observed. . Calreticulin physically interacts with the autophagic protein LC3 through the LIR motif. A, alignment of the LIR. B, calreticulin, but not its ⌬LIR form, physically interacted with LC3. HEK293T cells were cotransfected with the indicated constructs. GSH-agarose beads were utilized to immunoprecipitate GST-tagged proteins, and immunoblotting (IB) was carried out to determine the coprecipitated proteins. C, the interaction between calreticulin and LC3 was increased under ER stress. HEK293T cells were cotransfected with the indicated constructs and treated with vehicle (DMSO) or gradient dosages of tunicamycin or thapsigargin for 16 h. GSH-agarose beads were then utilized to immunoprecipitate GST-tagged proteins, and immunoblotting was carried out to determine the coprecipitated proteins. D, densitometric quantification of coprecipitated GFP-calreticulin in C (normalized to GFP-calreticulin protein levels in cell lysates). Shown are representative images of immunofluorescence (scale bars ϭ 10 m). E and F, the colocalization of calreticulin and LC3 is enhanced in response to tunicamycin treatment. HeLa cells were transfected with myc-CRT and GFP-LC3 constructs and treated with tunicamycin (2 g/ml) or vehicle (DMSO) for 6 h, followed by treatment with 50 M chloroquine for an additional 10 h as indicated. myc-CRT was labeled with red fluorescence. Confocal microscopy analysis was performed. Representative cells (E) and quantification of Pearson's correlation coefficient for colocalization between CRT and GFP-LC3 (F) are shown. Data are presented as the mean Ϯ S.E.; n ϭ 5. *, p Ͻ 0.05 versus chloroquine and vehicle.

Calreticulin induces autophagy under ER stress
These results indicate that ER stress may increase the association between calreticulin and LC3 via the conserved LIR motif.

The association between calreticulin and LC3 is required for calreticulin-mediated augmentation of autophagy and reduction of ER stress
To further investigate the potential role of calreticulin/LC3 interaction in the regulation of autophagy, we expressed myc-CRT WT or the ⌬LIR variant in HeLa cells and treated the cells with tunicamycin or thapsigargin. Autophagic flux assays were performed using chloroquine, a robust inhibitor of lysosomal hydrolases, to accumulate autophagosomal LC3-II. As shown in Fig. 6, A and B, in contrast to calreticulin WT-expressing cells, calreticulin-induced expression of LC3-II was largely abrogated in calreticulin ⌬LIR variant-expressing cells, suggesting a dramatic decline in autophagic flux. Consistently, calreticulin ⌬LIR variant-expressing cells exhibited less GFP-LC3 puncta compared with calreticulin WT-expressing cells under the same treatment in immunofluorescence imaging experiments (Figs. 4C and 6D). These data suggest that the LIR motif is required for calreticulin in the up-regulation of autophagic activity.
We next checked the ER stress levels in calreticulin ⌬LIRexpressing HeLa cells compared with calreticulin WT-expressing cells under treatment with tunicamycin. The calreticulin ⌬LIRexpressing cells presented a slight improvement in GRP78 signals as opposed to the attenuated bands in calreticulin WT-expressing cells (Fig. 6C). These findings indicate destruction of the protective role of calreticulin against ER stress conditions caused by the abolishment of calreticulin/LC3 interaction.
Taken together, we propose that up-regulation of autophagy might be the mechanism underlying calreticulin-repressed ER stress. To verify the protective role of autophagy, genetic approaches to activate or inhibit autophagy levels by overexpressing or knocking down the key autophagy components Atg5 or LC3 were performed. As shown in Fig. 7, HeLa cells with higher autophagy levels showed relatively low GRP78 levels compared with control cells, indicating their stronger tolerance of ER stress. This difference was increased under ER stress conditions. In contrast, knockdown of LC3 using shRNA caused induction of GRP78, suggesting increased ER stress. These data support the notion that autophagy activation mediates calreticulin's beneficial effects on alleviation of ER stress.

Discussion
This study demonstrates that calreticulin is a novel ER stress-inducible gene and exerts a negative feedback control system on alleviating ER stress. Mechanistically, calreticulin ameliorates ER stress in an autophagy-dependent manner, and

Calreticulin induces autophagy under ER stress
the association between calreticulin and LC3 is critical for calreticulin's effects on augmentation of autophagy and repression of ER stress. Calreticulin-mediated regulation of autophagy and ER stress may represent a molecular mechanism by which ER stress is sensed to maintain the cellular proteostasis state.

Calreticulin acts as a critical component in the negative feedback regulation of ER stress
One of the most important findings of the study is the identification of the ER-resident chaperone calreticulin as a novel regulator of ER stress. The UPR is a critical process to restore protein homeostasis under ER stress. A variety of chaperones are increased in the ER stress-induced UPR to attenuate the stress burden (7,38). Activation of these pathways leads to a complex cascade of downstream signaling, such as protein folding, ERAD, and autophagy (11). Consistent with a previous study (34), we found that calreticulin is up-regulated both on the transcriptional and translational levels under ER stress by in vivo and in vitro approaches. Interestingly, knockdown of calreticulin increases the expression of GRP78 in cells treated with ER stress inducers that lead to aggravation of stress burden. In contrast, calreticulin overexpression ameliorates the stress burden in parallel experiments, and these results suggest that calreticulin is necessary and sufficient to reverse the ER stress conditions. In this study, we proved that calreticulin is an UPRinduced chaperone and plays a protective role under ER stress. Further study of the upstream regulation of calreticulin is needed. It has been reported that the ATF6 -NF-Y key stress factor ATF4 is known as a chaperone activator (5,39,40). Whether ATF6 or ATF4 plays a role in the regulation of calreticulin is of interest for investigation.

Calreticulin serves as a potent activator of autophagy
Unfolded or misfolded proteins are targeted to ERAD pathways when their conformations cannot be restored by cellular chaperones (4,41). In addition, autophagy protects cells from adverse metabolic conditions, and it is considered a noncanonical ERAD pathway (11). Based on these facts, numerous previous studies proposed the definition of ER stress-stimulated autophagy (23-25, 42, 43), which are consistent with the observation in our study showing increased autophagy in the livers of mice treated with tunicamycin. Consistent with the previous  HeLa shLC3). HeLa shNC and shLC3 were lysed to perform an immunoblot assay. E, schematic of the calreticulin-autophagy axis in the negative feedback regulation of ER stress. Calreticulin is induced by the UPR in response to ER stress. Calreticulin enhances autophagic flux to attenuate cellular stress, likely through alleviation of aberrantly folded proteins. Therapeutic approaches to activate calreticulin-autophagy signaling may have potential for treating ER stress and its related disorders.
Another novel finding of the study is that activation of calreticulin increases the autophagic levels under ER stress. In this study, we demonstrate that calreticulin increases the levels of LC3-II in cells treated with ER stress inducers, which indicates increased autophagic levels. These data suggest that calreticulin may induce autophagic machinery to alleviate misfolded proteins during ER stress and maintain a cellular proteostasis state, which is consistent with previous observations showing a protective role of autophagy against ER stress (23,24). These results show that, in addition to retaining the misfolded glycoprotein in the folding cycle for refolding or degradation, calreticulin may alleviate cellular stress by stimulating autophagy signaling. The findings may provide a novel mechanism for chaperone-mediated activation of autophagy.

Calreticulin stimulates autophagy to suppress ER stress by enhancing its interaction with LC3
As a key autophagic protein, LC3 plays an essential role in autophagosome formation, elongation, and fusion with lysosomes (14,44). It has been reported that autophagy receptors associate with LC3 via the conserved LIR motif (45,46) and facilitate degradation of protein targets (47,48). Importantly, we demonstrate that calreticulin is required for the activation of autophagy under ER stress and identified, for the first time, that calreticulin interacts with LC3 in the conserved LIR motif of calreticulin protein. Coimmunoprecipitation and immunofluorescence assays proved that calreticulin ⌬LIR, a LIR motifdeficient mutant form, is not able to interact with LC3. Overexpression of calreticulin ⌬LIR abolishes calreticulin-mediated autophagic flux and fails to ameliorate drug-induced ER stress. These results demonstrate that the LIR motif is necessary for the calreticulin and LC3 interaction and is of great importance for the autophagy-dependent protective role of calreticulin under ER stress. Interestingly, we noticed that the binding affinity between calreticulin and LC3 is enhanced gradually when the cellular stress levels are increased by treatment with increasing dosages of tunicamycin or thapsigargin, which may represent augmentation of calreticulin-LC3 function in alleviating ER stress. These data suggest that ER stress and autophagy are causally linked via the interaction between calreticulin and LC3.
Moreover, this study characterizes that calreticulin acts as a novel LC3-binding adapter protein and that the calreticulin-LC3 complex appears to colocalize in autophagosomes, as visualized by confocal microscopy analysis. It is likely that calreticulin facilitates nuclear export of LC3 or increases conversion of LC3-I to LC3-II, leading to induction of autophagic flux (49). The detailed mechanisms of the calreticulin-LC3 complex regarding the activation of autophagy require further investigation.
We noticed that, although HeLa cells express a significant amount of endogenous CRT, the genetic approach to activate CRT activity, such as transient transfection of the plasmid encoding CRT, is sufficient to induce autophagy and repress ER stress. During ER stress, misfolded glycoproteins are accumu-lated in the ER, which requires a large amount of chaperone protein such as CRT for refolding, which is supported by the observation that endogenous CRT levels are induced under tunicamycin or thapsigargin treatment. Therefore, addition of exogenous CRT protein may facilitate the axis of CRT-mediated stimulation of autophagic flux. Together, these results support a critical role of CRT in maintaining proteostasis in response to cellular stress.
In summary, this study delineates a novel mechanism of the positive regulation of calreticulin in UPR-triggered autophagy under ER stress (Fig. 7E). The intriguing finding of interaction between calreticulin and LC3 has provided us with new insight into the role of calreticulin, coupling ER stress to autophagy by interacting with degradation machinery. Given that activation of the UPR and autophagy protects against insulin resistance and hepatic steatosis (2,32,33), the finding of a calreticulin-autophagy axis in the negative feedback regulation of ER stress may provide potential new therapeutic avenues for treating stress-related disorders.

Reagents and antibodies
Tunicamycin (catalog no. T7765), thapsigargin (catalog no. T9033), and chloroquine (catalog no. C6628) were purchased from Sigma-Aldrich, and puromycin, used in stable cell line screening, was purchased from Selleck Chemicals (catalog no. S7417). The calreticulin antibody was purchased from Millipore (catalog no. MABT145), and LC3 antibodies were purchased from Novus Biologicals (catalo no. NB100-2220) and Cell Signaling Technology (catalog no. 2775). The GRP78 antibody was purchased from Santa Cruz Biotechnology (catalog no. sc-13968). The GST pulldown procedure was performed using GSH-Sepharose 4B beads (GE Healthcare Bio-Sciences) according to the manufacturer's instructions.

Plasmids and siRNA
myc-tagged calreticulin and calreticulin ⌬LIR were constructed by cloning the human cDNAs into the KpnI/NotI sites of the pcDNA-myc-His B vector. GFP-tagged calreticulin and calreticulin ⌬LIR were constructed by cloning the human cDNAs into the XhoI/ApaI sites of the pEGFP-N2 vector. A two-step site-directed mutagenesis process using the KOD Plus Mutagenesis kit (Toyobo, catalog no. SMK-101) was performed to generate the human calreticulin ⌬LIR plasmid in which the tryptophan at the 200th amino acid and leucine at the 203rd amino acid were substituted with alanine (Trp 200 to Ala 200 , Leu 203 to Ala 203 ). The following primers were used: Trp 200 to Ala 200 , GCTCCTTGGAAGACGATGCGGACTTCCTGCC-ACCC (forward) and CGGACTCCACCTGGCTGTTGTC (reverse); Leu 203 to Ala 203 , GCCACCCAAGAAGATAAAGG-ATCCT (forward) and GCGAAGTCCCAATCGTCTTCCAA-GGAG (reverse). FLAG-tagged Agt5 was constructed by cloning human Agt5 cDNA into the NotI/BamHI sites of the pCMV10 -3ϫFLAGvector.FLAG-taggedhumanLC3wereconstructed by cloning LC3 cDNA into the SalI/NotI sites of the pcDNA-FLAG-SBP vector. The vectors pRK5-GST and pEGFP-C1 were modified and constructed to express LC3 with the SalI/NotI restriction site. The vectors psPAX2 and pMD2.G

Calreticulin induces autophagy under ER stress
were used to package knockdown lentiviruses. shRNAs were designed using the siRNAs above and cloned into pLKO.1, purchased from Addgene, according to the manufacturer's instructions. The sequences of CRT siRNAs were as follows (5Ј-3Ј): siCRT#1, AATCCGTCCAGAAACTGCTCC; siCRT#2, AAG-GAGCAGTTTCTGGACGGA; siLC3: AGCTCATCAAGAT-AATTAGAA. myc-tagged calreticulin was constructed by cloning calreticulin cDNA into the KpnI/NotI sites of pcDNA. All oligonucleotides were ordered from and synthesized by Shanghai Sunny Biotechnology Co. Ltd. The plasmids were sequenced by Genewiz Co.

Cell culture and transient transfection
HeLa and HEK293T cells were maintained in complete Dulbecco's modified Eagle's medium, high-glucose (DMEM (pH 7.3), Life Technology, catalog no. 12100046) supplemented with 10% fetal bovine serum (Biological Industries, catalog no. 04-001-1ACS), 100 g/ml penicillin, and 100 g/ml streptomycin (Life Technology, catalog no. 15140122) at 37°C in a humidified incubator with 5% CO 2 . Cells were cultured at a confluency of about 80%. To perform transient transfection, cells were seeded on proper plates or dishes to achieve a confluency of 70 -80%. Media were replaced into basal DMEM (pH 7.3) without fetal bovine serum or antibiotics. Plasmids were transfected into cells with polyethyleneimine (Sigma-Aldrich, catalog no. 408727) according to the manufacturer's instructions. Media were replaced with fresh complete DMEM 4 -6 h after transfection, and cells were harvested and subjected to the following assays about 24 h after transfection.

Lentivirus production, infection, and selection
Lentiviral particles were generated as described previously (31,50). Briefly, cells were transfected with the lentiviral transfer plasmid pCDH-CMV expressing the target gene, along with the packaging plasmids pMDLg/pRRE and pRSV-Rev (1:1 ratio) and the envelope plasmid pMD2.G using polyethyleneimine). The medium containing lentiviral particles was stored at 4°C. For lentivirus infection, cells were cultured, treated with lentiviral particle solution, and then selected and passaged under puromycin-containing medium for 4 -7 days. The resulting cells stably expressing the target gene were ready for further assays.

Animal model
Male C57BL/6 mice at 8 weeks of age were purchased from Shanghai Laboratory Animal Co. Ltd. Tunicamycin injection in mice was performed as described previously (52)(53)(54). Briefly, tunicamycin (Sigma-Aldrich, catalog no. 7765) was suspended at 0.05 mg/ml in PBS (150 mM) and injected intraperitoneally (1 g/g of body weight). Control mice were injected with PBS. The mice were sacrificed 48 h post-injection. The livers were harvested, homogenized, and lysed for immunoblots. All mice were housed under a 12:12-h light/dark cycle at controlled temperature. All animal experimental protocols were approved by the Institutional Animal Care and Use Committee at the Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

Immunoblots and GST pulldown
Immunoblot analysis was performed as described previously (55,56). The intensity of bands was quantified using ImageJ (National Institutes of Health, Bethesda, MD). For the GST pulldown analysis, cell lysates containing overexpressed GST-tagged fusion proteins or endogenous proteins were incubated with GSH-Sepharose 4B beads (GE Healthcare Bio-Sciences) at 4°C overnight and washed as co-immunoprecipitation. The precipitates were then analyzed by immunoblots.

Fluorescence microscopy
Cells were seeded onto glass coverslips. Forty-eight hours after transfection, cells were fixed in 4% paraformaldehyde, and coverslips were mounted in ProLong Gold antifade reagent with DAPI (Life Technologies) to visualize the nuclei. Images were captured under a confocal microscope (Olympus FV1200). Quantitative analyses of colocalization were done using Image Pro Plus software (Media Cybernetics, Inc.) with calculation of Pearson's correlation coefficient (51,58). Identical settings were used to capture images across five separate area per condition.

Statistical analysis
Data are expressed as mean Ϯ S.E. Statistical significance was evaluated using unpaired two-tailed Student's t test and among more than two groups by one-way analysis of variance. Differences were considered significant at p Ͻ 0.05.