Cardiolipin-induced activation of pyruvate dehydrogenase links mitochondrial lipid biosynthesis to TCA cycle function

Cardiolipin (CL) is the signature phospholipid of mitochondrial membranes. Although it has long been known that CL plays an important role in mitochondrial bioenergetics, recent evidence in the yeast model indicates that CL is also essential for intermediary metabolism. To gain insight into the function of CL in energy metabolism in mammalian cells, here we analyzed the metabolic flux of [U-13C]glucose in a mouse C2C12 myoblast cell line, TAZ-KO, which is CL-deficient because of CRISPR/Cas9-mediated knockout of the CL-remodeling enzyme tafazzin (TAZ). TAZ-KO cells exhibited decreased flux of [U-13C]glucose to [13C]acetyl-CoA and M2 and M4 isotopomers of tricarboxylic acid (TCA) cycle intermediates. The activity of pyruvate carboxylase, the predominant enzyme for anaplerotic replenishing of the TCA cycle, was elevated in TAZ-KO cells, which also exhibited increased sensitivity to the pyruvate carboxylase inhibitor phenylacetate. We attributed a decreased carbon flux from glucose to acetyl-CoA in the TAZ-KO cells to a ∼50% decrease in pyruvate dehydrogenase (PDH) activity, which was observed in both TAZ-KO cells and cardiac tissue from TAZ-KO mice. Protein–lipid overlay experiments revealed that PDH binds to CL, and supplementing digitonin-solubilized TAZ-KO mitochondria with CL restored PDH activity to WT levels. Mitochondria from TAZ-KO cells exhibited an increase in phosphorylated PDH, levels of which were reduced in the presence of supplemented CL. These findings indicate that CL is required for optimal PDH activation, generation of acetyl-CoA, and TCA cycle function, findings that link the key mitochondrial lipid CL to TCA cycle function and energy metabolism.

The phospholipid cardiolipin (CL) 8 is the signature lipid of mitochondrial membranes. It interacts with a wide variety of mitochondrial proteins (1) and has multiple roles in mitochondrial biogenesis as well as other cellular functions (2)(3)(4). Loss of CL leads to deficiencies in respiratory function, mitochondrial membrane potential, and ATP synthesis (1,(5)(6)(7). CL that is synthesized de novo has predominantly saturated fatty acids (8). However, the lipid is then remodeled via phospholipase-mediated deacylation to form monolysocardiolipin (MLCL), which is subsequently acylated by the enzyme tafazzin to synthesize CL with predominantly unsaturated fatty acids (9,10).
Abnormal CL composition is associated with several human disorders, including diabetes, Alzheimer's disease, and Parkinson's disease (11)(12)(13). However, Barth syndrome (BTHS) is the only disorder identified to date that is caused by altered CL metabolism (14,15). BTHS is a severe X-linked disorder characterized by dilated cardiomyopathy, skeletal myopathy, neutropenia, exercise intolerance, lactic acidosis, and sudden death from arrhythmia (16 -18). The underlying cause of BTHS is mutations in the tafazzin (TAZ) gene, which codes for the transacylase that remodels CL (10,19). As a result, BTHS patients exhibit an aberrant CL profile characterized by decreased total CL, increased MLCL, and abnormal CL acylation patterns (20). Tetralinoleoyl-CL, the most prevalent CL species in mitochondria of the human heart and skeletal muscle cells, is virtually absent from BTHS cells (21).
Although the clinical phenotypes of BTHS point to mitochondrial bioenergetic defects, the molecular basis whereby CL deficiency leads to the pathology is not understood. Several studies suggest that metabolic dysregulation is a key patholog-ical mechanism in BTHS. A clear distinction has been shown in plasma metabolite profiles from BTHS patients compared with control patients of similar age (22). For example, 3-methylglutaconic aciduria is commonly observed in BTHS patients, sometimes accompanied by increased levels of lactic acid (23)(24)(25)(26). 3-Methylglutaconic aciduria occurs as a result of increased excretion of 3-methylglutaconic and 3-methylglutaric acid, both of which are products of leucine catabolism (27). In addition, the organic acid 2-ethylhydracrylic acid has been reported in the urine of BTHS patients, indicating altered isoleucine metabolism (28,29). Furthermore, the levels of skeletal muscle phosphodiesters, intermediates of membrane phospholipid breakdown, were elevated in BTHS patients, indicative of a damaged sarcolemma, atrophied muscle, and loss of fiber (30,31).
Although the specific mechanisms leading to the metabolic changes in BTHS are unknown, they suggest that CL, long known to be required for oxidative phosphorylation and bioenergetics (2,3), is also required for optimal intermediary metabolism. This functional role is supported by studies in yeast strongly suggesting that CL deficiency leads to defects in the TCA cycle (32,33). Raja et al. (32) showed that yeast cells that cannot synthesize CL (crd1⌬) exhibit decreased levels of acetyl-CoA. Interestingly, although expression of the acetyl-CoA biosynthetic enzyme pyruvate dehydrogenase (PDH) is up-regulated in the yeast mutant, PDH activity is not increased, suggesting that optimal PDH catalytic activity may require CL. The PDH complex catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA by three catalytic activities: PDH (E1), dihydrolipoamide acetyltransferase (E2), and dihydrolipoamide dehydrogenase (E3) (34, 35). PDH kinases (PDKs) (36,37) and PDH phosphatases (PDPs) (38) are also components of mammalian PDH complexes. Flux of carbon through the PDH complex is tightly regulated by the phosphorylation state of the enzyme, which is controlled by PDKs and PDPs in response to altered metabolic conditions (35,39). Importantly, phosphorylation by PDKs of three sites in the E1␣ subunit leads to inactivation of the PDH complex; activity is restored by PDP-mediated dephosphorylation (40 -42). A second finding linking CL to the TCA cycle was that of Patil et al. (33), who reported that Fe-S biogenesis is perturbed in CL-deficient yeast cells. This is reflected in decreased activity of Fe-S-requiring enzymes, including the TCA cycle enzymes aconitase and succinate dehydrogenase (SDH). Consistent with perturbation of the TCA cycle, a recent report showed that anaplerotic pathways are required to ameliorate TCA cycle dysfunction in yeast cells (43). These discoveries, linking CL and TCA cycle function in yeast, raise the possibility that CL may play an essential role in metabolic regulation associated with BTHS pathology. Indeed, CL has been reported to be required for the stability and optimal enzymatic activity of SDH (44), and, accordingly, decreased protein levels and activity of SDH were also observed in cardiac tissue from BTHS mice (45).
To gain insight into the role of CL in energy metabolism, we analyzed the metabolic flux of [U-13 C]glucose in a tafazzin knockout mouse cell line, TAZ-KO (46). In this study, we demonstrate that the TCA cycle is perturbed in tafazzin-deficient cells, which exhibit decreased flux of glucose carbon to acetyl-CoA, TCA cycle metabolites, and related amino acids. We further show that CL activates PDH, supporting the hypothesis that decreased flux of carbon from glucose to acetyl-CoA in CL-deficient cells results from decreased PDH activity. To our knowledge, this is the first demonstration that the mitochondrial lipid CL is required for PDH activation, acetyl-CoA synthesis, and TCA cycle function in mammalian cells.

Decreased flux of carbon from glucose to acetyl-CoA in TAZ-KO cells
To investigate metabolic changes resulting from tafazzin deficiency, TAZ-KO and isogenic WT cells were cultured in medium containing [U-13 C]glucose, and glycolytic and TCA pathway metabolites in cell extracts were analyzed by LC-MS and GC-MS. [U-13 C]glucose was metabolized quickly to glycolytic intermediates during fermentation in both WT and TAZ-KO cells. However, the relative percentage of [ 13 C]acetyl-CoA was sharply reduced in TAZ-KO cells (Fig. 1A). Consistent with decreased flux of [U- 13 (Fig. 2).

Increased PC activity in TAZ-KO cells
PC is an anaplerotic enzyme that replenishes TCA cycle intermediates by catalyzing the conversion of pyruvate to OAA (47). Elevated PC activity (Fig. 3A) and increased levels of PC protein (Fig. 3B) were observed in TAZ-KO cells. In the presence of phenylacetic acid, an inhibitor of PC (48), growth of TAZ-KO cells was decreased, whereas growth of WT cells was not affected (Fig. 3C). These data suggest that PC is required to replenish the TCA cycle in TAZ-KO cells.
As all three carbons of [ 13 C]pyruvate generated from [U- 13 5B). Taken together, these experiments indicate that carbon flux from glucose to TCA cycle intermediates is reduced in TAZ-KO cells. This defect is partially compensated by up-regulation of PC, but compensation is limited by decreased SDH activity in the mutant.

PDH activity is decreased in tafazzin-deficient cells
To investigate the mechanism underlying diminished carbon flux from glucose to acetyl-CoA in mutant cells, we explored the possibility that PDH-mediated conversion of pyruvate to acetyl-CoA was defective. As glucose-derived pyruvate is the primary source of carbon for the generation of acetyl-CoA by PDH, decreased [ 13 C]acetyl-CoA in TAZ-KO cells suggested that PDH activity was decreased in the mutant. To test this possibility, PDH was assayed in mitochondrial extracts from TAZ-KO and WT cells. PDH activity was decreased by about 50% in TAZ-KO mitochondria (Fig. 6A), whereas the protein levels of PDH-E1␣ (the catalytic subunit of the PDH complex) were not decreased (Fig. 6B), suggesting that the PDH defect resulted from decreased catalytic activity of the enzyme. The deficiency in PDH activity was also apparent in cardiac tissue isolated from TAZ-KO mice (Fig. 6C), suggesting that our observations in myoblast cultures remain valid in a fully developed muscular organ such as the heart.
We further tested the in vivo activity of PDH in TAZ-KO cells by assaying the production of [ 14 C]CO 2 from [ 14 C]pyruvate. In agreement with the in vitro assay, TAZ-KO cells exhibited an ϳ50% decrease in PDH flux. When treated with the PDH kinase inhibitor dichloroacetate (DCA), which activates PDH flux, [ 14 C]CO 2 production in TAZ-KO cells was restored to WT levels ( Fig. 6D), suggesting that the reduced PDH flux in TAZ-KO cells is due to active inhibition rather than impaired capacity.

Cardiolipin activates PDH
To investigate the possibility that PDH is activated by CL, levels of which are reduced in TAZ-KO cells, digitonin-solubilized mitochondria from TAZ-KO and WT cells were incubated with exogenous CL. As seen in Fig. 7A, CL restored the activity of PDH in mutant mitochondria to WT levels. In agree- ment with this finding, incubation of CL with purified PDH complex resulted in an increase in enzymatic activity (Fig. 7B). Binding of CL to the PDH complex was assessed by an in vitro protein lipid overlay assay. Both CL and phosphatidic acid bound to the PDH complex ( Fig. 7C), but activation of PDH was specific for CL (Fig. 7B). MLCL, which accumulates in tafazzindeficient cells (46), did not stimulate activity (data not shown).
As discussed above, PDH is regulated by phosphorylation, which decreases the catalytic activity (40). We therefore addressed the possibility that CL activates PDH by facilitating a decrease in phosphorylation of the enzyme. Consistent with decreased PDH activity, mitochondria isolated from TAZ-KO cells exhibited an increase in phosphorylated PDH (Fig. 8A). When digitonin-solubilized TAZ-KO mitochondria were incubated with exogenous CL, phosphorylation of PDH was reduced (Fig. 8B).
Taken together, these experiments suggest that CL links mitochondrial lipid biosynthesis with energy metabolism by mediating a decrease in PDH phosphorylation and enhancing PDH activity. Deficient PDH activity in CL-deficient cells results in decreased synthesis of acetyl-CoA with concomitant perturbation of the TCA cycle.

Discussion
In this study, we show for the first time that CL is required for optimal activity of the acetyl-CoA biosynthetic enzyme PDH and TCA cycle function. CL-deficient TAZ-KO cells exhibited decreased PDH activity (Fig. 6), increased levels of the phosphorylated, inactive enzyme (Fig. 8A), and decreased flux of glucose to acetyl-CoA and TCA cycle intermediates (Figs. 1 and 2). A requirement for anaplerosis was demonstrated in the mutant by up-regulation of PC and sensitivity to PC inhibition (Figs. 3 and 4). These findings identify a regulatory link between the mitochondrial membrane lipid CL and the reactions of energy metabolism in the mitochondrial matrix.
Our findings suggest that the mechanism whereby CL regulates PDH is that of facilitating a decrease in PDH phosphorylation. In agreement with this, supplementation of digitonin-

CL regulates the TCA cycle
solubilized mitochondria with exogenous CL led to a decrease in phosphorylated PDH. Dephosphorylation of PDH is facilitated by Ca 2ϩ -dependent binding of PDP to the L2 domain of the E2 subunit dihydrolipoyl acetyltransferase (50,51). This suggests the possibility that CL, which has a high affinity for Ca 2ϩ (52), facilitates binding of PDP to the E2 subunit, increasing dephosphorylation of the enzyme. This mechanism would be consistent with an early report showing that the PDH complex binds tightly to the mitochondrial inner membrane (53). Our findings do not rule out the possibility that CL may inhibit PDK activity, resulting in decreased phosphorylation of the enzyme. Experiments to test these possible mechanisms are in progress. An increase in the protein level and cellular activity of PC was observed in TAZ-KO cells (Fig. 3, A and B). The importance of PC in TAZ-KO cells is reflected in the delayed growth of these cells in the presence of the PC inhibitor phenylacetic acid (Fig.  3C). A previous study reported that increased PC is necessary for continued mitochondrial metabolism and cellular anabolism in SDH mutant human cells (54). These findings suggest that TCA cycle perturbation in TAZ-KO cells is anaplerotically rescued by PC.
The decrease in SDH activity in TAZ-KO cells (Fig. 5A) is in agreement with the finding of heart-specific SDH deficiency in the BTHS mouse model (45). We have shown previously that yeast crd1⌬ cells, which lack CL, exhibit decreased activity of  This study may have important implications for BTHS. Defects in PDH activity and perturbation of the TCA cycle are expected to lead to a reduction in ATP production and energy deficits, causing cardiomyopathy and heart failure (55,56). Increased expression of PC may be essential to compensate for energy deficits resulting from decreased acetyl-CoA synthesis. PDH deficiency further results in increased generation of lactic acid, which may contribute to the observed lactic acidosis and organic aciduria. Importantly, BTHS is characterized by a wide disparity in symptoms, even among patients with identical tafazzin mutations, suggesting that physiological modifiers of tafazzin deficiency contribute to the clinical presentation of the disease (57). We speculate that PDH, PC, and SDH are likely candidates for physiological modifiers of BTHS, as defects in these enzymes may exacerbate the effects of tafazzin deficiency. In conclusion, this study identifies a multifunctional role of CL in cellular metabolism and may shed light on the wide disparities in clinical phenotypes observed in BTHS patients.

CRISPR/Cas9 off-target analysis of TAZ-KO cells
The TAZ-KO cell line used in this study was generated previously using CRISPR/Cas9 targeted against exon 3 of the tafazzin (Taz) gene in C2C12 mouse myoblasts (46). The top 10 predicted off-target sites were identified using the CCTop CRISPR/Cas9 target predictor tool (https://crispr.cos.uniheidelberg.de/) 9 based on the Mus musculus GRCm38 refer-ence genome and the gRNA sequence (5Ј-TCCTAAAACTCC-GCCACATC-3Ј) utilized to create the TAZ-KO cell line (46,63). Next-generation whole-genome sequencing was performed on WT and TAZ-KO cells, and a differential variant analysis was run to identify deviations between the WT and TAZ-KO genomes (GENEWIZ Biotechnology, South Plainfield, NJ). The readout from the differential variant analysis lists all genomic loci (also mapped to the M. musculus GRCm38 reference genome) at which the sequence of the TAZ-KO cell line differs from that of the WT cell line. The genomic coordinates of each of the 10 predicted off-target sites as well as the on-target Taz locus were manually compared against the differential variant analysis data. This analysis indicated that none of the predicted off-target sites were altered in the TAZ-KO cell line relative to isogenic WT cells (Table S1).

Mouse heart tissue preparation
Mouse protocols were approved by the Institutional Animal Care and Use Committee of the New York University School of Medicine. Mice (C57BL/6) were housed under temperaturecontrolled conditions and a 12-h light/dark cycle with free access to drinking water and food. The TAZ-KO mouse model was developed in the C57BL/6 background by Dr. D. Strathdee of the Cancer Research UK Beatson Institute and is available upon request. TAZ-KO mice and WT mice were euthanized to harvest their hearts. The hearts were dissected, and the tissue pieces were stored at Ϫ80°C before measurement of PDH activity.

Cell proliferation assay
3000 cells were suspended in 100 l of growth medium and seeded into 96-well plates. Viable cells were measured in triplicate using an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Fisher) assay after 3, 24, and 48 h. In brief, 10 l of 5 mg/ml MTT was added to each well. As a negative control, MTT was applied to wells lacking cells. The plate was incubated for 4 h at 37°C. The medium was carefully removed, and 150 l of DMSO was added to dissolve the MTT product. The plate was covered with foil and incubated for 10 min at 37°C. Samples from each well were mixed with a pipette, and absorbance was read at 570 nm.

Immunoblotting
Cell or mitochondrial extract corresponding to 50 g of protein was analyzed using a 10% SDS-PAGE gel. Immunoblotting was performed using primary antibodies to PDH-E1 (1:1000, Santa Cruz Biotechnology, sc-377092, lot D1615), phos-PDH (1:1000, Millipore, AP1062, lot 2923467), PC (1:1000, Santa 9 Please note that the JBC is not responsible for the long-term archiving and maintenance of this site or any other third party-hosted site.

CL regulates the TCA cycle
Cruz Biotechnology, sc-271862, lot G1514), NDUFB6 (1:5000), and actin (1:3000) (Santa Cruz Biotechnology). Corresponding secondary antibodies conjugated to horseradish peroxidase were used. Immunoreactivity was visualized using ECL substrate (Thermo). To determine the effect of CL on PDH phosphorylation, aliquots of mitochondria (400 g of protein) were suspended in 40 ml of 50 mM NaCl and 50 mM imidazole HCl (pH 7.0). 2 l of CL (bovine heart, Sigma) from a stock solution containing 20 mg/ml in 5% digitonin and 50% ethanol and 1 ml of digitonin (10% in water) was added to obtain a CL/protein ratio of 1:10 (g/g) and a digitonin/protein ratio of 0.5 (g/g). After 2-h incubation on ice, levels of phosphorylated PDH were determined by Western blot analysis with antibody against phos-PDH (Millipore).

Mitochondrial extraction
Cells were cultured in 150-mm dishes until they reached 100% confluency, collected, and centrifuged at 720 ϫ g for 5 min. Cell pellets were washed with cold PBS and suspended in mitochondrial isolation buffer (280 mM sucrose, 0.25 mM EDTA, and 20 mM Tris-HCl (pH 7.2)) and manually homogenized with a glass homogenizer. Cell debris was removed by centrifugation at 720 ϫ g for 5 min. Mitochondria were subsequently collected by centrifugation at 14,812 ϫ g for 10 min.
Protein concentration was determined using a detergent compatible protein assay kit (Bio-Rad).

Metabolomic flux analysis
Metabolomic flux analysis was carried out at the University of Michigan Metabolomics Core Services as follows.
Central carbon metabolism sample preparation-Cell culture plates were removed from Ϫ80°C storage and maintained on wet ice throughout the processing steps. To each 6-cm plate, 0.5 ml of a mixture of methanol, chloroform, and water (8:1:1) was added. Plates were gently agitated and then scraped to release cells, which were transferred to a microtube, vortexed, and incubated at 4°C for 10 min to complete metabolite extraction. Samples were vortexed a second time and then centrifuged at 22,000 ϫ g for 10 min at 4°C. After centrifugation, 100 l of the extraction solvent was transferred to an autosampler vial for LC-MS analysis. 10 l of each sample was removed and pooled in a separate autosampler vial for quality control analyses. The remaining supernatant from each sample was transferred to an autosampler vial and dried. To each sample, 50 l of a 20 mg/ml solution of methoxyamine hydrochloride in pyridine was added. Samples were vortexed briefly, incubated at 37°C for 90 min, removed from heating, and allowed to cool to room temperature. 50 l of N-methyl-N-tert-butyldimethyl- silyltrifluoroacetamide) with 1% tertbutyldimethylchloro silane (Regis Technologies) was added, and samples were incubated at 70°C for 1 h. A series of calibration standards was prepared along with samples for quality control of instrument performance and chromatography for LC and GC analysis.
LC-MS analysis-LC-MS analysis was performed on an Agilent system consisting of a 1260 ultra-high performance liquid chromatography module coupled with a 6520 Quadrupole-TOF mass spectrometer (Agilent Technologies). Metabolites were separated on a 150 ϫ 1 mm Luna NH 2 hydrophilic interaction chromatography column (Phenomenex) using 10 mM ammonium acetate in water, adjusted to pH 9.9 with ammonium hydroxide as mobile phase A and acetonitrile as mobile phase B. The flow rate was 0.075 ml/min, and the gradient was linear (20% to 100% A) over 15 min, followed by isocratic elution at 100% A for 5 min. The system was returned to starting conditions (20% A) in 0.1 min and held there for 10 min to allow for column re-equilibration before injecting another sample. The mass spectrometer was operated in electrospray ionization mode according to conditions published previously (58).
GC-MS analysis-GC-MS analysis was performed on an Agilent 69890N GC-5975 MS detector with the following parameters. A 1-l sample was injected splitlessly on an HP-5MS 15m column (Agilent Technologies, Santa Clara, CA) with a He gas flow rate of 1.4 ml/min. The GC oven initial temperature was 60°C, was increased at 10°C per minute to 300°C, and held at 300°C for 5 min. The inlet temperature was 250°C, and the MS source and quad temperatures were 230°C and 150°C, respectively.
Data analysis-Metabolites were identified by matching the retention time and mass to authentic standards. For LC-MS, metabolites were identified using Agilent's Profinder Isotopologue extraction program, which does automatic isotope subtraction based on the expected isotope distribution for the molecular formula provided. For GC-MS data, metabolites were identified by matching the retention time and mass (0.1-Da resolution) to authentic standards. Isotope peak areas were integrated using MassHunter Quantitative Analysis vB.07.00 (Agilent Technologies). Peak areas were corrected for natural isotope abundance (using a software package written in-house based on the method of Fernandez et al. (58)), and the residual isotope signal was reported.

Protein lipid overlay assay
Protein lipid overlay assays were performed according to the protocol of Dowler et al. (59) with modification. In brief, lyophilized lipids (tetraoleoyl-CL, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-trisphosphate, and phosphatidic acid) were dissolved in a 1:1 solution of methanol and chloroform to make 1 mM stocks. Lipids were diluted to five different concentrations (500, 250, 125, 62.5, and 31.25 M) in a 2:1:0.8 solution of methanol:chloroform:water. Then 1 l of lipid sample from each dilution was spotted on a nitrocellulose membrane. After Figure 7. CL binds to PDH and increases its activity. A, digitonin-solubilized mitochondria from WT and TAZ-KO cells were treated with or without CL. PDH activity was assayed as described under "Experimental procedures." B, purified PDH complex (Sigma) was incubated with exogenous CL or phosphatidic acid (PA). PDH activity was assayed as described under "Experimental procedures". Data shown are mean Ϯ S.D. (n ϭ 3). *, p Ͻ 0.05. C, the indicated lipids CL, phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol 4,5-bisphosphate (PIP 2 ), phosphatidylinositol 3,4,5-trisphosphate (PIP 3 ), and phosphatidic acid were serially diluted and spotted onto a nitrocellulose membrane, which was incubated overnight in buffer containing 10 g of PDH complex. Interactions were detected by immunoblotting with an antibody against the PDH complex.

CL regulates the TCA cycle
drying for 1 h at room temperature, membranes were incubated in blocking buffer (50 mM Tris-HCl (pH 7.5), 150 mM NaCl 2 , 0.1% Tween 20, and 2 mg/ml BSA) with gentle rocking for 1 h at room temperature and then incubated overnight at 4°C with gentle rocking in 10 ml of blocking buffer containing 25 g of purified PDH complex (Sigma). PDH bound to lipids on the membrane was detected by immunoblotting using primary antibody (Assaypro) against the PDH complex (1:1000) and secondary antibody conjugated to horseradish peroxidase (1:3000).

Enzyme assays
In vivo assay of PDH activity-Pyruvate oxidation was determined by measuring the release of 14 CO 2 from [1-14 C]pyruvate as described previously (60). Cells were seeded in glass vials at ϳ100 g/vial in DMEM (without glucose, pyruvate, or glutamine/1% FCS). The following day, cells were washed twice with Dulbecco's PBS prior to 3-h incubation in Dulbecco's PBS (Life Technologies) supplemented with 100 M [1-14 C]pyruvate (specific activity, 0.1 Ci/ml; Perkin Elmer Life Sciences). A center well containing 2 M NaOH was placed to trap CO 2 . After 1 h of shaking at 37°C, medium was acidified with 2.6 M perchloric acid at a final concentration of 0.4 M to stop the reaction. After 3 h of trapping, the 14 CO 2 collected in the center well was counted by liquid scintillation. Pyruvate oxidation flux was determined by the amount of pyruvate oxidized to CO 2 normalized to protein content.
In vitro assay of PDH activity-Mitochondrial PDH activity was measured spectrophotometrically by determining the reduction of NAD ϩ to NADH, coupled to the reduction of a reporter dye to yield a colored reaction product with an increase in absorbance at 450 nm at 37°C (Biovision). PDH enzyme activity was expressed in units per milligram of total protein. To determine the effect of phospholipids on PDH activity, aliquots of mitochondria (400 g of protein) were suspended in 40 ml of 50 mM NaCl and 50 mM imidazole HCl (pH 7.0). 2 l of CL (from bovine heart, Sigma), phosphatidic acid (Avanti), or MLCL (Avanti) from a stock solution containing 20 mg/ml in 5% digitonin and 50% ethanol and 1 ml of digitonin (10% in water) were added to obtain a phospholipid/protein ratio of 1:10 (g/g) and a digitonin/protein ratio of 0.5 (g/g). After 2-h incubation on ice, 10 ml of digitonin (10% stock solution in water) was added to obtain a digitonin/protein ratio of 3:1 (g/g), which was sufficient for quantitative solubilization. The samples were used directly in PDH activity assays. To determine the effect of phospholipids on purified PDH (Sigma) activity, 2 ml of CL or phosphatidic acid from a stock solution containing 20 Figure 8. CL decreases levels of phosphorylated PDH. Phosphorylated PDH was identified by Western blot analysis with an antibody against the phosphorylated enzyme (Phos-PDH). The mitochondrial protein NDUFB6 was included as a loading control. For DCA-treated samples, 5 mM DCA was added to cultured WT cells for 24 h before mitochondrial isolation. A, levels of phosphorylated PDH in mitochondrial protein from WT and TAZ-KO cells. B, levels of phosphorylated PDH in digitonin-solubilized mitochondria from WT and TAZ-KO cells incubated for 2 h on ice with or without CL. Bottom panels, the signal intensities of protein bands and surrounding background were scanned and quantified using ImageJ. The background-subtracted value for each protein band was normalized to that of NDUFB6 and quantified relative to the DCA-treated sample.
CL regulates the TCA cycle mg/ml in 5% digitonin and 50% ethanol was incubated with purified PDH complex for 10 min at room temperature.
MDH activity-MDH activity was measured as oxidation of NADH, determined by a decrease of absorbance at 340 nm. The assays were performed in buffer containing 100 mM KPi, 0.1 mM NADH, and 0.2 mM oxaloacetate. After stabilization, Triton X-100 solubilized mitochondria were added, and the absorbance was measured at 340 nm.
PC activity-PC activity was measured spectrophotometrically by monitoring the formation of CoA at 412 nm as modified from the protocol of Payne and Morris (61). The assay was performed in buffer containing 1 M Tris-HCl (pH 8), 0.5 M NaHCO 3 , 0.1 M MgCl 2 , 1 mM acetyl-CoA, 0.1 M pyruvate, 0.1 M ATP, 0.0039 g of 5,5Ј-dithiobis-(2-nitrobenzoic acid), and 1000 units/ml citrate synthase. After stabilization, the reaction was started by adding Triton X-100 -solubilized mitochondria.