Prion disease is accelerated in mice lacking stress-induced heat shock protein 70 (HSP70)

Prion diseases are a group of incurable neurodegenerative disorders that affect humans and animals via infection with proteinaceous particles called prions. Prions are composed of PrPSc, a misfolded version of the cellular prion protein (PrPC). During disease progression, PrPSc replicates by interacting with PrPC and inducing its conversion to PrPSc. As PrPSc accumulates, cellular stress mechanisms are activated to maintain cellular proteostasis, including increased protein chaperone levels. However, the exact roles of several of these chaperones remain unclear. Here, using various methodologies to monitor prion replication (i.e. protein misfolding cyclic amplification and cellular and animal infectivity bioassays), we studied the potential role of the molecular chaperone heat shock protein 70 (HSP70) in prion replication in vitro and in vivo. Our results indicated that pharmacological induction of the heat shock response in cells chronically infected with prions significantly decreased PrPSc accumulation. We also found that HSP70 alters prion replication in vitro. More importantly, prion infection of mice lacking the genes encoding stress-induced HSP70 exhibited accelerated prion disease progression compared with WT mice. In parallel with HSP70 being known to respond to endogenous and exogenous stressors such as heat, infection, toxicants, and ischemia, our results indicate that HSP70 may also play an important role in suppressing or delaying prion disease progression, opening opportunities for therapeutic intervention.


Prion diseases are a group of incurable neurodegenerative disorders that affect humans and animals via infection with proteinaceous particles called prions. Prions are composed of PrP Sc , a misfolded version of the cellular prion protein (PrP C ).
During disease progression, PrP Sc replicates by interacting with PrP C and inducing its conversion to PrP Sc . As PrP Sc accumulates, cellular stress mechanisms are activated to maintain cellular proteostasis, including increased protein chaperone levels. However, the exact roles of several of these chaperones remain unclear. Here, using various methodologies to monitor prion replication (i.e. protein misfolding cyclic amplification and cellular and animal infectivity bioassays), we studied the potential role of the molecular chaperone heat shock protein 70 (HSP70) in prion replication in vitro and in vivo. Our results indicated that pharmacological induction of the heat shock response in cells chronically infected with prions significantly decreased PrP Sc accumulation. We also found that HSP70 alters prion replication in vitro. More importantly, prion infection of mice lacking the genes encoding stress-induced HSP70 exhibited accelerated prion disease progression compared with WT mice. In parallel with HSP70 being known to respond to endogenous and exogenous stressors such as heat, infection, toxicants, and ischemia, our results indicate that HSP70 may also play an important role in suppressing or delaying prion disease progression, opening opportunities for therapeutic intervention.
Prions are the causative transmissible agents for a group of neurodegenerative disorders, including scrapie in sheep, bovine spongiform encephalopathy in cattle, chronic wasting disease in cervids, and Creutzfeldt-Jakob disease (CJD) 3 in humans. The key component of a prion is PrP Sc , which is the misfolded form of the cellular prion protein (PrP C ). During disease, PrP Sc self-propagates by serving as a template for the conformational conversion of PrP C substrate (1). Through an incompletely characterized and complex mechanism, PrP Sc accumulation triggers cellular and endoplasmic reticulum (ER) stress (2). One of the initial cellular responses to PrP Sc -induced stress is the up-regulation of specific glucose-regulated proteins (GRPs), protein disulfide isomerases, and heat shock proteins (HSPs) that function as molecular chaperones and folding enzymes (2). Together, these proteins generally assist with proper protein folding, inhibit protein aggregation, and direct the degradation of misfolded proteins.
HSPs belong to a large multigene family and are grouped into subfamilies according to their molecular mass, which can range from 8 to 150 kDa (3,4). Many HSPs are expressed under normal physiological conditions. These levels can increase dramatically in response to stress, especially stressors that increase production of aberrant proteins. The HSP70 subfamily is highly evolutionarily conserved and has members present in all subcellular compartments (ER, cytoplasm, and nucleoplasm) to mediate protein quality control (3,4).
The initial possibility of a relationship between HSPs and mammalian prion diseases stems from work in the yeast prion field. Yeast prions have been defined as non-Mendelian genetic elements (e.g. [URE3], [PSI ϩ ], [Het-s], and [PIN ϩ ]) found to be infectious prion forms of host-encoded proteins (i.e. Ure2p, Sup35p, HET-s, and Rnq1p, respectively) (5-7). Generally speaking, yeast prions are protein aggregates with high ␤-sheet content that form amyloids and self-propagate this altered conformational state both vertically (as genes composed of protein) and horizontally (as infectious mammalian prions). The first connection between HSPs and yeast prions was the discovery that HSP104 specifically induces their fragmentation, thus enhancing self-propagation (8). Moreover, overproduction or inactivation of HSP104 cured the yeast cells of prions without affecting viability. Therefore, a certain level of disaggregation mediated by HSP104 is necessary to maintain yeast prion propagation, but too much disaggregation completely destroys the replicating prion seeds (8). Although HSP104 is the disaggregase, the primary guide that delivers HSP104 to the yeast prions is HSP70 (9 -12), which is a function that can be assisted by HSP40 (13)(14)(15). It has been difficult to translate our knowledge about yeast prions to mammalian prions because the mammalian genome does not encode a corresponding HSP104 homolog. Furthermore, despite the ability of yeast HSP104 to induce mammalian PrP Sc propagation in vitro (16), prion inoculation of transgenic mice expressing yeast heat shock protein 104 did not modulate PrP Sc accumulation or disease incubation time (17). In summary, the current model in yeast states that HSP104 and some members of the HSP70 and HSP40 families are working together in fibril fragmentation and prion propagation in yeast (18).
Among the chaperones discovered to be elevated in CJD patients (19 -22) and scrapie-infected rodents (23)(24)(25) was HSP70, which is the original prototype of the larger HSP70 subfamily encoded in mammals by the nearly identical Hspa1a and Hspa1b genes. In mice and other mammals, the HSP genes Hspa1a and Hspa1b are induced by both endogenous and exogenous stressors, such as heat and toxicants. They have been shown to be the major and usually only HSP70 members that respond to most physiologically relevant stresses. The Hspa1a/ Hspa1b genes differ by a single amino acid and are divergent from all other members of the larger HSP70 gene family, particularly in the C-terminal domain, which contains the substrate binding site. The Hspa1a/Hspa1b genes are not to be confused with the Hsf1 gene that encodes a transcription factor. Knockout of the Hspa1a/Hspa1b genes eliminates their endogenous or induced response to stressors, although other HSP70 gene members are present at endogenous levels. Furthermore, these HSP70 gene family members are the only ones whose cytosolic levels increase in response to stress, further strengthening the potential for a functional role in prion disease.
The physiological role of HSP70 in prion disease has been unclear because it has yet to be specifically explored in a bona fide model for mammalian prion disease. In a previous study, we investigated the role of HSP70 using a model in transgenic Drosophila overexpressing PrP C that naturally accumulated PrP aggregates with aging (26). This insoluble PrP form induced neurotoxicity and locomotor dysfunction, similar to PrP Sc during prion disease (26). In these transgenic flies, HSP70 was discovered to reduce the accumulation of PrP aggregates by ϳ40% and alleviate the associated neurological deficits. Although HSP70 is a cytosolic/nuclear chaperone and PrP C is a cell surface protein, evidence indicated that the positive effect from HSP70 was mediated by a direct interaction with PrP (26). If such a scenario indeed takes place in a mammalian host, then the lack of HSP70 induction, a major target of heat shock factor (HSF1), may explain why Hsf1 Ϫ/Ϫ knockout mice succumb to prion disease ϳ20% faster than WT controls (27). Here we used an in vitro model of PrP C -to-PrP Sc conversion, a mammalian cell culture model, and Hsp70 knockout mice (Hspa1a Ϫ/Ϫ and Hspa1b Ϫ/Ϫ , hereafter referred to as Hsp70 Ϫ/Ϫ ) to better understand the role of HSP70 in prion infection.

Effect of a drug-induced heat shock response on PrP Sc in cell culture
The 17-(dimethylaminoethylamino)-17-demethoxygeldanamycin (17-DMAG) drug is a water-soluble derivative of geldanamycin known to induce a strong heat shock response in mammalian cells (28). More specifically, 17-DMAG exhibits the broad ability to induce expression of molecular chaperones, including cytosolic HSP40, HSP70, HSP90, and HSP105 as well as GRP94 in the ER. Following our previously established treatment regimen (29,30), RK13 cells were treated at the beginning and middle of a 6-day period with 60-min exposure to 0, 0.1, 0.5, or 1.5 M 17-DMAG. We observed a strong induction of HSP70 in RK13 cells treated with 0.5 and 1.5 M 17-DMAG, which was absent for untreated cells and those treated with 0.1 M 17-DMAG (Fig. 1A). This is in agreement with our previous results indicating that 17-DMAG induces HSPs and could be detected for 3 to 5 days after transient exposure. 4 Furthermore, BCA protein analysis for these treatments demonstrated that 1.5 M 17-DMAG resulted in a modest reduction in protein content as a result of toxicity, so we chose to utilize 0.5 M or less for all future treatments to avoid a confounding effect.
RK13 cells are a versatile prion cell culture model that has successfully been genetically engineered to propagate prions derived from sheep (31)(32)(33), mice (32,34,35), bank voles (34), or cervids (32,36). Using clonal selection on the previously established RKM7 line expressing mouse PrP C (32), a clone highly sensitive to Rocky Mountain Laboratory (RML) mouseadapted scrapie prions was isolated and chronically infected, referred to as RKM7-RML. RKM7 and RKM7-RML cells were treated with 0, 0.1, or 0.5 M 17-DMAG to determine the effect of a heat shock response on prion infection. Interestingly, prion-infected cells treated with 0.5 M 17-DMAG demonstrated a 50% reduction in PrP Sc (p Ͻ 0.001; Fig. 1, B and C). A similar 17-DMAG treatment did not alter PrP C levels in RKM7 cells (Fig. 1D), suggesting that the effect is at the level of prion replication. These results indicate that the drug-induced heat shock response was sufficient to significantly reduce PrP Sc in RKM7-RML cells. In future studies, we will attempt to analyze in detail the correlation between the magnitude of the changes of HSP70 expression and the reduction in PrP Sc replication.

Influence of HSP70 on prion disease onset and progression
We specifically focused on the role of HSP70 in vivo because it is the only HSP induced by 17-DMAG that is also known to be elevated in CJD patients (37) and scrapie-infected mice (24). For this reason, we intracranially inoculated normal brain homogenate (NBH) or RML prions into mice deficient in HSP70 protein (Hsp70 Ϫ/Ϫ ) and WT controls expressing HSP70 (Hsp70 ϩ/ϩ ; Fig. 2A). HSP70-null animals developed terminal prion disease significantly faster compared with WT controls (Fig. 2B), as analyzed by log-rank (Mantel-Cox) test (p Ͻ 0.05).

The role of HSP70 in prion disease
The mean incubation period (inoculation to death) for Hsp70 Ϫ/Ϫ mice (145.2 Ϯ 3.06 days) was also significantly shorter than for Hsp70 ϩ/ϩ mice (156.0 Ϯ 3.29 days, p Ͻ 0.05; Fig. 2B). These results indicate that HSP70 has a protective role in the onset of prion disease. To further investigate whether HSP70 may also affect the progression of the disease, we blindly analyzed the rate of clinical changes in the two groups of infected animals. Onset and progression of clinical abnormalities were evaluated by a semiquantitative scale commonly employed in the field to analyze clinical signs of prion disease in rodents (38,39). When normal animals transition from stage 1, the first abnormalities observed in RML prion-infected mice include lack of nesting, rough coat on limbs, and abnormal social behavior (stage 2). This is followed by extensive rough coat, hunchback, and visible motor abnormalities (stage 3). Stage 4 includes more severe progression of previous signs plus urogenital lesions, increased motor abnormalities, and extensive weight loss. At the end stage of the disease (stage 5), animals present with severe ataxia and usually lie in the cage with little movement. For this analysis, stage 6 was scored at termination, after consistently maintaining a score of 5 and as they began to progress further. Clinical signs were first noted for all RML-challenged animals in both groups 123 days post-inoculation (dpi, Fig. 2C). Surprisingly, disease progression began to diverge from this point onward; Hsp70 Ϫ/Ϫ mice exhibited significantly more rapid progression through the clinical stages until becoming terminal (140 dpi, p Ͻ 0.01; 144 dpi, p Ͻ 0.05; 146 dpi, p Ͻ 0.01; 147 dpi, p Ͻ 0.001; 148 dpi, p Ͻ 0.001; 150 dpi, p Ͻ 0.01). In conclusion, our results suggest that the absence of HSP70 reduced the incubation period in prion disease by accelerating clinical progression.

Absence of HSP70 does not affect pathological hallmarks
The major hallmark of prion disease is proteinase K (PK)resistant PrP Sc deposition, which is accompanied by vacuolation and astrogliosis in the brain. Equal amounts of PrP Sc with an identical banding pattern were observed by Western blot analysis for Hsp70 Ϫ/Ϫ and Hsp70 ϩ/ϩ mice terminal with RML prion disease (Fig. 3A). The equal amounts of pathological protein were not unexpected because these lines express indistinguishable PrP C levels (Fig. 3B), and animals were sacrificed at the same stage of the disease in both groups. In parallel, histological analysis for Hsp70 Ϫ/Ϫ and Hsp70 ϩ/ϩ fixed paraffin-embedded samples revealed the typical diffuse PrP Sc deposition expected for RML prion disease (Fig. 3C). Moreover, both lines exhibited similar profiles of vacuolation (Fig. 4A) and gliosis (Fig. 4B). Overall, the pathology was most prominent in the thalamus, cerebellum, and hippocampus. These results indicate that the absence of HSP70 does not affect the accumulation or molecular properties of PrP Sc or the pathological hallmarks of RML prion disease despite altering clinical progression.

Cell-free PrP Sc replication is affected by the presence of HSP70 in the substrate
Infectious prions can be generated in vitro by protein misfolding cyclic amplification (PMCA), where PrP C is converted

The role of HSP70 in prion disease
into PrP Sc with high efficiency utilizing cyclic bursts of sonication (40). This assay can be set up to enable the detection of minute quantities of PrP Sc for diagnosis or to estimate the concentration of PrP Sc present for quantification (41). We utilized this assay to determine whether the absence of HSP70 altered prion conversion by seeding PMCA reactions with RML prions in NBH substrate from three individual Hsp70 Ϫ/Ϫ or Hsp70 ϩ/ϩ mice. Because PrP C levels are equivalent in the two substrates (Fig. 3B), the primary difference in these reactions is the presence or absence of active HSP70 plus any compensatory changes resulting from the removal of this protein (e.g. overexpression of other chaperone proteins). To our surprise, Hsp70 ϩ/ϩ substrate from three individual animals consistently supported the generation of PrP Sc following two rounds of PMCA seeded with a 10 Ϫ8 dilution of RML, whereas no PrP Sc was detected in a similar reaction with Hsp70 Ϫ/Ϫ substrate (Fig.  5). However, PrP Sc amplification became nearly indistinguishable after a third round. In summary, more PK-resistant PrP Sc is produced in Hsp70 ϩ/ϩ substrate than in Hsp70 Ϫ/Ϫ substrate, suggesting that kinetics (rather than eventual yield) were influenced by HSP70.

Discussion
In this study, we examined the role of HSP70 in prion replication using an in vitro model of PrP C to PrP Sc conversion, a mammalian cell culture model, and a rodent model for prion disease. There is extensive literature supporting the activation of cellular stress mechanisms to regain proteostasis during prion disease (2). One such mechanism is elevated levels of chaperones and foldases triggered to correct the increasing number of misfolded proteins. Indeed, high HSP70 levels have been reported in CJD patients (37) and scrapie-infected mice (24). Moreover, clarifying the influence of HSP70 in mammalian prion disease is particularly intriguing because another member of the HSP family (HSP104) specifically induces fragmentation of yeast prions, enhancing their self-propagation (8). Although the mammalian genome does not encode a corresponding homologue of HSP104, the current model in yeast and Hsp70 ϩ/ϩ mice. TH states for B, survival of Hsp70 Ϫ/Ϫ and Hsp70 ϩ/ϩ mice challenged with RML prions or normal brain homogenate (controls) from a healthy animal was determined. Data were analyzed by log-rank (Mantel-Cox) test and found to be significant with p Ͻ 0.05. C, prion disease progression was evaluated on a scale of 1 to 6 as described under "Experimental procedures." A rating of 1 represents healthy animals, and a rating of 6 was used to designate clinically sick animals at the moment when they were sacrificed. Data were analyzed by two-way analysis of variance, using time and type of mice as the variables, and the differences were significant with p Ͻ 0.0001. Individual differences were analyzed by Bonferroni post-test. *, p Ͻ 0.05; **, p Ͻ 0.01; *** p Ͻ 0.001.

The role of HSP70 in prion disease
demonstrates that HSP104 and some members of the HSP70 and HSP40 families are working together in fibril fragmentation and prion propagation (18). Together, our data demonstrate that HSP70 may also play an important role in regulating mammalian prion disease.
Our cell culture experiments broadly determined that a heat shock response is beneficial in reducing PrP Sc levels. In fact, chemical induction of a heat shock response by incubation with 17-DMAG significantly reduced PrP Sc levels in a cell culture model for prion disease by 50%. However, expression of the molecular chaperones HSP40, HSP70, HSP90, GRP94, and HSP105 has been reported to be induced by treatment with 17-DMAG in mammalian cells (28). Although our data confirmed increased levels of HSP70 under our experimental conditions, the complete effect cannot be solely attributed to this chaperone protein. To the best of our knowledge, previous data from in vitro or in vivo studies have yet to link HSP40 and HSP105 to prion disease, but a possible contribution cannot be ruled out. In addition, the induction of the unfolded protein response (UPR) may have impacted this result as well. This finding is also particularly unique because a heat shock response could be induced in normal prion-free neuroblastoma cells but not neuroblastoma cells propagating prions (42). Furthermore, an additional pitfall in experiments using prion-infected cells is the well-established clonal instability of neuroblastoma cells to keep infection (43). This deficiency has been less impactful for prion-infected lines derived from RK13 cells, where each RKM7-RML cell has been shown to contain detectable levels of PrP Sc and not just a small fraction of the popula-tion (44). Moreover, it is noteworthy that the unaltered amount of PrP C in 17-DMAG-treated RKM7 cells provides additional evidence that the reduction in PrP Sc is not an artifact of drug toxicity but the result of the heat shock response.
Although our cell culture data do not allow us to causally link the observed effects specifically to the loss of stress-induced HSP70, this effect enabled us to narrow our investigation to HSP70 because it is the only known HSP induced by 17-DMAG to also be elevated in CJD patients (37) and scrapie-infected mice (24). Impressively, deletion of Hsp70 in vivo significantly shortened the incubation period for RML prion infection. The accelerated disease produced by knocking out HSP70 may be explained by impairment of prion degradation and/or enhancement of PrP Sc accumulation. However, animals lacking HSP70 may also exhibit enhanced sensitivity to prion disease because HSP70 is known to be a powerful inhibitor of apoptosis by binding the apoptotic factor apoptotic protease activating factor 1 (APAF-1) (45) as well as apoptosis-inhibitory factor (46). To further investigate the effect of HSP70 in prion disease, we carefully monitored clinical progression of the disease in both groups of infected mice. The results clearly showed that the difference in prion incubation periods was the result of accelerated clinical progression of the disease in Hsp70-null animals. Indeed, Hsp70 ϩ/ϩ or Hsp70 Ϫ/Ϫ mice infected with RML prions began to develop clinical signs of the disease at the same time, but Hsp70 Ϫ/Ϫ mice deteriorated much faster. The accelerated clinical progression observed in the absence of HSP70 suggests that this chaperone is active in preventing prion toxicity. Because prion disease is divided into an infectivity phase and a Figure 3. Evaluation of PrP in Hsp70 ؊/؊ and Hsp70 ؉/؉ mice. A, Western blot analysis was used to detect PK-resistant PrP Sc in brain homogenates from five individual Hsp70 Ϫ/Ϫ and HSP70 ϩ/ϩ mice at the end stage of prion disease. B, a similar Western blot analysis was used to detect PrP C in brain homogenate derived from five individual uninfected mice with GAPDH as the loading control. C, histological analysis of PrP Sc accumulation in fixed brain tissue from representative RML-infected Hsp70 Ϫ/Ϫ and Hsp70 ϩ/ϩ mice, which were counterstained with hematoxylin to simultaneously observe cell nuclei. Scale bars ϭ 250 m.

The role of HSP70 in prion disease
toxicity phase (47, 48), HSP70 may primarily respond during the toxicity phase. The effect of HSP70 on clinical progression of prion disease observed in our study was comparable with a previous bioassay utilizing HSF1 knockout animals (27). In this study, Hsf1 Ϫ/Ϫ mice also exhibited faster clinical progression, indicating that HSP70 may be at least partially responsible because Hsp70 is known to be a major target of HSF1. Nonetheless, we need to be cautious when interpreting our results because it has been shown that the impact of altered genotypes on prion disease progression requires a larger number of animals than those used in our study to obtain definitive conclusions (49,50).
An interesting observation in the animal infectivity experiments was that there were no changes in the type of clinical signs, PK-resistant PrP Sc , vacuolation, or astrogliosis related to the presence or absence of HSP70. From these results, we can conclude that the absence of HSP70 did not promote generation of a new prion strain. In many ways, this was the expected outcome because strain changes from selection or evolution have only been reported in cases where the PrP C sequence in the host differed from the donor PrP Sc sequence, the species barrier was crossed, or resistance to anti-prion drug treatment was observed (51). Although additional experiments would be required, the putative mechanism by which HSP70 altered the clinical course of the disease might be related to changes on PrP Sc replication or, more likely, to the cell's ability to withstand the insult of PrP Sc toxicity.
Our in vitro studies of prion replication provide evidence that HSP70 may alter PrP C -to-PrP Sc conversion. However, these experiments need to consider the impact tissue homogenization and cell lysis have on PrP Sc propagation. For example, an interaction between HSP70 and PrP Sc could be induced artificially by lysing the cells, whereas under physiological conditions this may not occur. Nonetheless, colocalization was observed between PrP and HSP70 in transgenic flies (26), and interaction analyses provide evidence that other chaperones (i.e. GRP58 and PDIA1) function by directly binding to PrP (21,23,52,53). Also, some redundancy may exist for the molecular chaperones involved in prion disease; thus, other chaperones may play an augmented role upon removal of HSP70. The roles of these chaperones could similarly be exaggerated during in vitro prion replication studies. A simple interpretation of our PMCA results may be that HSP70 alters the kinetics of PrP Sc conversion per se. Even with all of the potential technical issues . Histological changes in RML prion-infected Hsp70 ؊/؊ and Hsp70 ؉/؉ mice. A, spongiform degeneration was analyzed in paraffin-embedded brain tissue slices from Hsp70 Ϫ/Ϫ and Hsp70 ϩ/ϩ mice inoculated with RML prions. As controls, we used animals of both genotypes injected with NBH from a healthy animal. Slices were stained with H&E. B, gliosis was evaluated in fixed brain samples from Hsp70 Ϫ/Ϫ and Hsp70 ϩ/ϩ mice inoculated with RML prions or NBH by probing with anti-glial fibrillary acidic protein (GFAP) antibody. Cell nuclei were simultaneously counterstained with hematoxylin. For the studies in this figure, we focused on the thalamus, hippocampus, and cerebellum, areas heavily affected by RML prion infection. Scale bars ϭ 250 m.

The role of HSP70 in prion disease
discussed above, it seems that our data may, in fact, be sufficient for favoring an effect (direct or indirect) on prion conversion/ replication as the most likely cause of the effect observed during the clinical phase of disease in vivo.
Compared with our mouse model of prion disease, overexpression of HSP70 in Drosophila more effectively prevented PrP accumulation and/or promoted the degradation of aggregated PrP to an extent that largely reversed neurological deficits (26). A major difference in these models is that the mice in this study were infected with bona fide prions, whereas the transgenic flies exhibited age-dependent PrP accumulation as a consequence of overexpression. Like PrP Sc in mammalian prion disease, fly PrP aggregates induced spongiform degeneration, resisted high concentrations of denaturing agents, and contained PrP Sc -specific conformational epitopes (26). Despite these similarities, the fly PrP aggregates were mostly proteasesensitive. Therefore, HSP70 may effectively target a neurotoxic and protease-sensitive PrP Sc isoform, a function potentially compromised by the more heterogeneous PrP Sc population in prion-infected mice. Concomitant with the results generated in the transgenic flies, the appearance of disease symptoms in mice has been shown to depend on increasing levels of small protease-sensitive oligomeric PrP Sc instead of larger proteaseresistant aggregates (47). For this reason, it would be interesting to determine the impact of HSP70 specifically on small protease-sensitive oligomeric PrP Sc generated in our mice.
Further strengthening the evidence that this pathway is specifically involved in prion disease, we also recently showed that another HSP70 family member localized in the ER, GRP78/Bip, similarly influences prion incubation (54). A comparison of the impact of HSP70 and GRP78 is intriguing because they are both members of the larger HSP70 protein family, with ϳ63% amino acid identity. Both are also reportedly activated during prion disease (2). Although the complete absence of HSP70 accelerated the incubation time by ϳ7%, reducing GRP78 expression by 50% resulted in 20% shorter incubation times with the same prion strain (54). Substrate specificity may contribute to the shortened incubation time because individual HSP70 family members are likely evolutionally optimized to recognize and bind different peptide sequences or structures. Alternatively, GRP78 may seemingly play a more prominent role in prion disease because of localization in the ER lumen, whereas HSP70 is mainly cytosolic. However, the localization differences may be questioned because the source of toxicity in prion disease and the impact of ER misfolded PrP on PrP Sc propagation are controversial topics (2). Another possibility is that, in addition to its activity as a molecular chaperone, GRP78 also acts as a master regulator of the unfolded UPR pathway (39). Thus, the larger effect of GRP78 in prion disease may in part be due to an indirect activity on launching the UPR. Indeed, a substantial amount of evidence exists in favor of the UPR, particularly the Protein Kinase RNA-like Endoplasmic Reticulum Kinase pathway, playing a critical role during prion disease (55)(56)(57). Last, GRP78 levels could possibly be affected in HSP70 knockout mice because both proteins are involved in stress response, and some HSP may influence expression of the other family members.
Our study represents the first investigation of a putative influence of HSP70 in a bona fide model of mammalian prion disease. Together, our data support the hypothesis that HSP70 helps reduce toxicity and extend disease incubation. These results complement previous literature utilizing prion bioassays in mice lacking HSF1 and studies with other molecular chaperones. Our findings may open up novel targets for therapeutic intervention in prion diseases.

Cell culture conditions and treatment
A rabbit kidney epithelial cell line (RK13, catalog no. CCL-37, ATCC) stably expressing mouse PrP C using pIRESpuro3 (Clontech), called RKM cells, was created previously (32). Here we utilized the RKM7 clone (generated from RKM cells) that was selected for high sensitivity to infection with the RML scrapie prion strain. Following chronic infection with RML, we refer to this line as RKM7-RML. RK13, RKM7, and RKM7-RML were grown in Dulbecco's modified Eagle's medium (Invitrogen) containing 10% fetal bovine serum and 1:100 antibioticantimycotic (Gibco) at 37°C with 5% CO 2 . For treatment, 1 ϫ 10 5 cells were seeded in a 6-well plate (ϳ10% confluence) and cultured overnight. The cells were treated with 17-DMAG for 60 min twice (first treatment on day 0, second treatment on day 3) over a 6-day period. For these two 60-min exposures, 17-DMAG (dissolved in PBS) or an equal volume of PBS was supplemented into the culture medium. Cells were washed immediately following each 60-min treatment to allow them to recover during the assay. In summary, each exposure was concluded by aspiration, a PBS wash, and complete replacement with fresh medium lacking the supplement. Cells were har- Figure 5. In vitro prion replication by PMCA using Hsp70 ؊/؊ and Hsp70 ؉/؉ brains as substrate. PMCA reactions were seeded with RML prions present in a 10 Ϫ8 dilution of brain homogenate from terminally ill mice. As substrate for PMCA, we used 10% brain homogenate from three individual Hsp70 Ϫ/Ϫ or Hsp70 ϩ/ϩ mice, and amplification continued for three consecutive rounds (see "Experimental procedures"). PrP Sc formation was evaluated by Western blotting after PK digestion. Non-PK-digested samples from NBH derived from a healthy mouse was used as a migration control.

Mouse lines, prion bioassays, and tissue harvesting
All mice were housed at the University of Texas Health Science Center at Houston in a facility accredited by the Association for Assessment and Accreditation of Laboratory Animal Care in accordance with animal protocols approved by the Institutional Animal Care and Use Committee. Mice genetically deficient in HSP70 (Hspa1a Ϫ/Ϫ and Hspa1b Ϫ/Ϫ , referred to as Hsp70 Ϫ/Ϫ ) were maintained as a homozygous colony, with genotyping by PCR using primers and conditions as reported previously (58), whereas WT animals sharing the same C57BL/6J genetic background (Hspa1a ϩ/ϩ and Hspa1b ϩ/ϩ , referred to as Hsp70 ϩ/ϩ ) were purchased from The Jackson Laboratories (code no. 664). Because of the high sensitivity of prions to genetic backgrounds, the C57BL/6J substrain was chosen because it is the exact genetic match for the animals in which the Hsp70 Ϫ/Ϫ mice were created. In brief, Hsp70 Ϫ/Ϫ mice were initially backcrossed by speed congenics onto this C57BL/6J strain for six generations and then further backcrossed onto the same strain by standard breeding for an additional four crosses. According to guidelines established by Prusiner et al. (59), groups of five female Hsp70 Ϫ/Ϫ and WT Hsp70 ϩ/ϩ mice (ranging in age from 72 to 78 days) were infected with the RML mouse-adapted scrapie strain via stereotactic intracranial inoculation of 10 l of 10% (w/v) brain homogenate (BH) from a terminally ill animal into the hippocampus (right hemisphere). Similarly, five age-matched mice for each line received an equal volume of 10% (w/v) NBH derived from a healthy animal. Animals were evaluated five times per week for health conditions and appearance of prionassociated clinical signs. Because RML infection has been carefully characterized in our laboratory, blind evaluation of the mice was conducted to score the progression of prion disease using the following version of a scale published previously (38, 39): 1, normal; 2, no nesting, rough coat on limbs, abnormal social behavior; 3, extensive rough coat, hunchback, and visible motor abnormalities; 4, progression of observed signs plus urogenital lesions, increased motor activity, and extensive weight loss; 5, end stage of disease where the animal presents ataxia and lies in the cage with little movement. Animals were terminated following consecutive scores of 5 as they became terminal. Upon termination, mouse brains were harvested by freezing the right cerebral hemisphere and fixing the left cerebral hemisphere in Carnoy fixative. Frozen samples were homogenized at 10% (w/v) in PBS containing complete protease inhibitors (Roche).

PMCA
PMCA was carried out according to our published protocol (41), using BH from Hsp70 Ϫ/Ϫ or Hsp70 ϩ/ϩ mice (both expressing equal WT levels of endogenous mouse PrP C ) as substrate. Briefly, brain substrate was prepared at a concentration of 10% (w/v) in conversion buffer (PBS supplemented with 150 mM NaCl and 1% Triton X-100) with protease inhibitors (Complete, Roche). Debris was removed by low-speed centrifugation (800 ϫ g, 1 min, 4°C) and stored at Ϫ80°C until use. For PMCA, aliquots of 10 l of 10% (w/v) BH from terminal RMLinfected mice, and 90 l of brain substrate were added to 0.2-ml PCR tubes (Eppendorf, catalog no. 951010022) containing two Teflon beads (Hoover Precision Products) and subjected to 48 PMCA cycles. Each cycle consisted of 29 min and 40 s incubation at 37/40°C, followed by a 20-s pulse of sonication set at a potency of 110 -120 W using the Qsonica microsonicator (model Q700) equipped with a titanium horn. Subsequent rounds of 48 PMCA cycles were done by taking an aliquot of the amplified material and diluting it 10-fold into fresh brain substrate. After four rounds of PMCA, PK-digested samples were analyzed by Western blots probed with the 6D11 anti-PrP antibody (1:30,000, BioLegend), as described previously (41).

Brain histology
The left cerebral hemisphere of the mouse brain was fixed in Carnoy fixative (60), dehydrated, and embedded in paraffin. 10-m slices were mounted on glass slides, dewaxed with xylene, and hydrated in solutions of decreasing concentrations of alcohol (100% to 70%). Serial sections were treated with 6% H 2 O 2 for 20 min, rinsed in water, and stained with H&E or immunostained with monoclonal antibodies for PrP (6H4, 1:1000, Prionics) or astrocytes (anti-glial fibrillary acidic protein (GFAP), 1:2000, Abcam). To visualize PrP Sc , the sections were treated with 10 g/ml PK for 5 min at room temperature and subsequently with 3 M guanidine isothiocyanate for 20 min at room temperature. To prevent nonspecific binding, the Animal Research Kit (Dako, Glostrup, Denmark) was used. Immunostaining was developed using HRP-conjugated streptavidin and visualized with 3-3Ј-diaminobenzidine (Vector Laboratories) as chromogen. Tissue was later counterstained with hematoxylin for 30 s and rinsed in tap water for 10 min. Sections were examined under a bright-field DMI6000B microscope (Leica).

Western blotting
Protein concentrations were quantified for cell lysate and BH using the BCA assay kit (Pierce). PrP C was analyzed in 40-g 10% (w/v) BH and 200-g cell lysate samples precipitated with 5 volumes of MeOH and resuspended in 1ϫ NuPAGE Lithium Dodecyl Sulfate sample loading buffer (Invitrogen). For PrP Sc , 40-g 10% (w/v) BH and 650-g cell lysate samples were digested with 100 g/ml PK for 1 h at 37°C with shaking, which was stopped by adding 1 mM phenylmethylsulfonylfluoride. Digested samples were centrifuged at 4°C for 1 h at 20,000 ϫ g, and pellets were resuspended in 1ϫ NuPAGE LDS sample loading buffer. All samples were boiled for 10 min before running in 14% Novex Tris/glycine gels (Invitrogen) and transferring onto nitrocellulose membranes (0.45 m, Amersham Biosciences). Blots were blocked for 1 h at room temperature with 5% milk. Then PrP was probed with the 6H4 mAb (1:10,000). Following incubation with anti-mouse IgG secondary antibody conjugated to horseradish peroxidase (1:3,000), the signal was visualized using the ECL chemicochemical reagent (Amersham Biosciences) and a Chemidoc imaging system (Bio-Rad). As a loading control, some blots were reprobed for GAPDH (anti-GAPDH, 1:2,000, Pierce) after stripping (Restore Western blotting stripping buffer, Thermo Scientific) and blocking. Similarly, HSP70 was detected in 20 g of BH (anti-HSP70

The role of HSP70 in prion disease
ADI-SPA-810, 1:2,000, Enzo Life Sciences) with tyrosine hydroxylase as a loading control (anti-tyrosine hydroxylase ab112, 1:2,000, Abcam). For analysis of cultured cells, ϳ10 g of total cell extract was probed with HSP70 antibody, and equivalent gel protein loading was confirmed by simultaneous probing with monoclonal mouse anti-actin (MP Biomedicals, 08691001, 1:100,000).

Statistical analysis
ImageJ was utilized to perform densitometry on the western blots. GraphPad Prism was used to calculate statistical significance via Tukey's multiple comparisons test for Western blots, Mann-Whitney test for in vivo incubation, and two-way analysis of variance for clinical progression. Statistical significance is shown as follows: *, p Ͻ 0.05; **, p Ͻ 0.01; and ***, p Ͻ 0.001.